|
Name |
Accession |
Description |
Interval |
E-value |
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
7-546 |
0e+00 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 911.25 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 7 EHELAPNEANHVPLSPLSFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEA 86
Cdd:PRK08162 5 EQGLDRNAANYVPLTPLSFLERAAEVYPDRPAVIHGDRRRTWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEA 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 87 HYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDREFGAVAQQALAMLDAP-PLLVGIDDDQAERADLAHDLDYEA 165
Cdd:PRK08162 85 HFGVPMAGAVLNTLNTRLDAASIAFMLRHGEAKVLIVDTEFAEVAREALALLPGPkPLVIDVDDPEYPGGRFIGALDYEA 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 166 FLAQGDPGRPLSAPQNEWQSIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAV 245
Cdd:PRK08162 165 FLASGDPDFAWTLPADEWDAIALNYTSGTTGNPKGVVYHHRGAYLNALSNILAWGMPKHPVYLWTLPMFHCNGWCFPWTV 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 246 TLSGGTHVCLRKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHA--SVPAVPVSVITGGAAPPSAVIAAMEARGFSIT 323
Cdd:PRK08162 245 AARAGTNVCLRKVDPKLIFDLIREHGVTHYCGAPIVLSALINAPAEwrAGIDHPVHAMVAGAAPPAAVIAKMEEIGFDLT 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 324 HAYGMTESYGPSTLCLWQPGVDELPLEARAQFMSRQGVAHPMLEEATVLDTDTGRPVPADGRTLGELVVRGNTVMKGYLH 403
Cdd:PRK08162 325 HVYGLTETYGPATVCAWQPEWDALPLDERAQLKARQGVRYPLQEGVTVLDPDTMQPVPADGETIGEIMFRGNIVMKGYLK 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 404 NPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAF 483
Cdd:PRK08162 405 NPKATEEAFAGGWFHTGDLAVLHPDGYIKIKDRSKDIIISGGENISSIEVEDVLYRHPAVLVAAVVAKPDPKWGEVPCAF 484
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 489250734 484 VALRADAlpRTTGDDLVRWCRERLAHFKAPRHVTLTELPKTATGKIQKFVLREWARQEEEAGD 546
Cdd:PRK08162 485 VELKDGA--SATEEEIIAHCREHLAGFKVPKAVVFGELPKTSTGKIQKFVLREQAKSLKAIDL 545
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
17-536 |
0e+00 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 762.61 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 17 HVPLSPLSFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAV 96
Cdd:cd12118 1 YVPLTPLSFLERAAAVYPDRTSIVYGDRRYTWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 97 LVCINIRLEARSIAFILRHCAARVLICDREFgavaqqalamldappllvgidddqaeradlahdlDYEAFLAQGDPGRPL 176
Cdd:cd12118 81 LNALNTRLDAEEIAFILRHSEAKVLFVDREF----------------------------------EYEDLLAEGDPDFEW 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 177 SAPQNEWQSIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLR 256
Cdd:cd12118 127 IPPADEWDPIALNYTSGTTGRPKGVVYHHRGAYLNALANILEWEMKQHPVYLWTLPMFHCNGWCFPWTVAAVGGTNVCLR 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 257 KVQPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVP--VSVITGGAAPPSAVIAAMEARGFSITHAYGMTESYGP 334
Cdd:cd12118 207 KVDAKAIYDLIEKHKVTHFCGAPTVLNMLANAPPSDARPLPhrVHVMTAGAPPPAAVLAKMEELGFDVTHVYGLTETYGP 286
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 335 STLCLWQPGVDELPLEARAQFMSRQGVAHPMLEEATVLDTDTGRPVPADGRTLGELVVRGNTVMKGYLHNPEATRAALAD 414
Cdd:cd12118 287 ATVCAWKPEWDELPTEERARLKARQGVRYVGLEEVDVLDPETMKPVPRDGKTIGEIVFRGNIVMKGYLKNPEATAEAFRG 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 415 GWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAlpRT 494
Cdd:cd12118 367 GWFHSGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLYKHPAVLEAAVVARPDEKWGEVPCAFVELKEGA--KV 444
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 489250734 495 TGDDLVRWCRERLAHFKAPRHVTLTELPKTATGKIQKFVLRE 536
Cdd:cd12118 445 TEEEIIAFCREHLAGFMVPKTVVFGELPKTSTGKIQKFVLRD 486
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
9-540 |
0e+00 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 661.15 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 9 ELAPNEANHVPLSPLSFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHY 88
Cdd:PLN02479 9 DLPKNAANYTALTPLWFLERAAVVHPTRKSVVHGSVRYTWAQTYQRCRRLASALAKRSIGPGSTVAVIAPNIPAMYEAHF 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 89 GVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDREFGAVAQQALAMLDA-------PPLLVGIDDDQAERADLAHDL 161
Cdd:PLN02479 89 GVPMAGAVVNCVNIRLNAPTIAFLLEHSKSEVVMVDQEFFTLAEEALKILAEkkkssfkPPLLIVIGDPTCDPKSLQYAL 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 162 -----DYEAFLAQGDPGRPLSAPQNEWQSIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHC 236
Cdd:PLN02479 169 gkgaiEYEKFLETGDPEFAWKPPADEWQSIALGYTSGTTASPKGVVLHHRGAYLMALSNALIWGMNEGAVYLWTLPMFHC 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 237 NGWSHTWAVTLSGGTHVCLRKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEhASVPAVP----VSVITGGAAPPSAVI 312
Cdd:PLN02479 249 NGWCFTWTLAALCGTNICLRQVTAKAIYSAIANYGVTHFCAAPVVLNTIVNAP-KSETILPlprvVHVMTAGAAPPPSVL 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 313 AAMEARGFSITHAYGMTESYGPSTLCLWQPGVDELPLEARAQFMSRQGVAHPMLEEATVLDTDTGRPVPADGRTLGELVV 392
Cdd:PLN02479 328 FAMSEKGFRVTHTYGLSETYGPSTVCAWKPEWDSLPPEEQARLNARQGVRYIGLEGLDVVDTKTMKPVPADGKTMGEIVM 407
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 393 RGNTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARP 472
Cdd:PLN02479 408 RGNMVMKGYLKNPKANEEAFANGWFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARP 487
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 489250734 473 DSRWGETPHAFVALRADAL---PRTTGDDLVRWCRERLAHFKAPRHVTLTELPKTATGKIQKFVLREWARQ 540
Cdd:PLN02479 488 DERWGESPCAFVTLKPGVDksdEAALAEDIMKFCRERLPAYWVPKSVVFGPLPKTATGKIQKHVLRAKAKE 558
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
9-545 |
0e+00 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 564.64 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 9 ELAPNEANHVPLSPLSFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHY 88
Cdd:PLN03102 3 NLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHF 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 89 GVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDREFGAVAQQALAMLDA------PPLLVGIDDDQAERAdLAHDLD 162
Cdd:PLN03102 83 AVPMAGAVLNPINTRLDATSIAAILRHAKPKILFVDRSFEPLAREVLHLLSSedsnlnLPVIFIHEIDFPKRP-SSEELD 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 163 YEAFLAQGDPGRPLSAP----QNEWQSIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNG 238
Cdd:PLN03102 162 YECLIQRGEPTPSLVARmfriQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHCNG 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 239 WSHTWAVTLSGGTHVCLRKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHA--SVPAVPVSVITGGAAPPSAVIAAME 316
Cdd:PLN03102 242 WTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLdlSPRSGPVHVLTGGSPPPAALVKKVQ 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 317 ARGFSITHAYGMTESYGPSTLCLWQPGVDELPLEARAQFMSRQGVAHPMLEEATVLDTDTGRPVPADGRTLGELVVRGNT 396
Cdd:PLN03102 322 RLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQMELKARQGVSILGLADVDVKNKETQESVPRDGKTMGEIVIKGSS 401
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 397 VMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRW 476
Cdd:PLN03102 402 IMKGYLKNPKATSEAFKHGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPTW 481
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 477 GETPHAFVAL---------RADALpRTTGDDLVRWCRERLAHFKAPRHVT-LTELPKTATGKIQKFVLREWAR----QEE 542
Cdd:PLN03102 482 GETPCAFVVLekgettkedRVDKL-VTRERDLIEYCRENLPHFMCPRKVVfLQELPKNGNGKILKPKLRDIAKglvvEDE 560
|
...
gi 489250734 543 EAG 545
Cdd:PLN03102 561 DNV 563
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
25-536 |
5.71e-165 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 478.53 E-value: 5.71e-165
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 25 FLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRL 104
Cdd:PRK06187 11 ILRHGARKHPDKEAVYFDGRRTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAVLHPINIRL 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 105 EARSIAFILRHCAARVLICDREFGAVAQQALAMLDAPPLLVGIDDDQAERADlAHDLDYEAFLAQGDPGRPlsAPQNEWQ 184
Cdd:PRK06187 91 KPEEIAYILNDAEDRVVLVDSEFVPLLAAILPQLPTVRTVIVEGDGPAAPLA-PEVGEYEELLAAASDTFD--FPDIDEN 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 185 SIAIN-YTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPDAI 263
Cdd:PRK06187 168 DAAAMlYTSGTTGHPKGVVLSHRNLFLHSLAVCAWLKLSRDDVYLVIVPMFHVHAWGLPYLALMAGAKQVIPRRFDPENL 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 264 HAAIAEHAVTHLSAAPVVMSMLIHAEHAsvPAVPVS----VITGGAA-PPSAVIAAMEARGFSITHAYGMTESyGPSTLC 338
Cdd:PRK06187 248 LDLIETERVTFFFAVPTIWQMLLKAPRA--YFVDFSslrlVIYGGAAlPPALLREFKEKFGIDLVQGYGMTET-SPVVSV 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 339 LWQPgvDELPleARAQFMSRQGVAHPMLEeATVLDTDtGRPVPADGRTLGELVVRGNTVMKGYLHNPEATRAALADGWLH 418
Cdd:PRK06187 325 LPPE--DQLP--GQWTKRRSAGRPLPGVE-ARIVDDD-GDELPPDGGEVGEIIVRGPWLMQGYWNRPEATAETIDGGWLH 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 419 TGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPrtTGDD 498
Cdd:PRK06187 399 TGDVGYIDEDGYLYITDRIKDVIISGGENIYPRELEDALYGHPAVAEVAVIGVPDEKWGERPVAVVVLKPGATL--DAKE 476
|
490 500 510
....*....|....*....|....*....|....*....
gi 489250734 499 LVRWCRERLAHFKAPRHVTL-TELPKTATGKIQKFVLRE 536
Cdd:PRK06187 477 LRAFLRGRLAKFKLPKRIAFvDELPRTSVGKILKRVLRE 515
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
24-545 |
2.60e-159 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 461.59 E-value: 2.60e-159
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 24 SFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIR 103
Cdd:COG0318 3 DLLRRAAARHPDRPALVFGGRRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLNPR 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 104 LEARSIAFILRHCAARVLICdrefgavaqqalamldappllvgidddqaeradlahdldyeaflaqgdpgrplsapqnew 183
Cdd:COG0318 83 LTAEELAYILEDSGARALVT------------------------------------------------------------ 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 184 qsIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSH-TWAVTLSGGTHVCLRKVQPDA 262
Cdd:COG0318 103 --ALILYTSGTTGRPKGVMLTHRNLLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVgLLAPLLAGATLVLLPRFDPER 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 263 IHAAIAEHAVTHLSAAPVVMSMLihAEHASVPAVPVS----VITGGAAPPSAVIAAMEAR-GFSITHAYGMTESYGPSTL 337
Cdd:COG0318 181 VLELIERERVTVLFGVPTMLARL--LRHPEFARYDLSslrlVVSGGAPLPPELLERFEERfGVRIVEGYGLTETSPVVTV 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 338 ClwqpgvdelPLEARAQFMSRQGVAHPMLEeATVLDtDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAALADGWL 417
Cdd:COG0318 259 N---------PEDPGERRPGSVGRPLPGVE-VRIVD-EDGRELPPG--EVGEIVVRGPNVMKGYWNDPEATAEAFRDGWL 325
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 418 HTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPrtTGD 497
Cdd:COG0318 326 RTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVVAFVVLRPGAEL--DAE 403
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 489250734 498 DLVRWCRERLAHFKAPRHVT-LTELPKTATGKIQKFVLREWARQEEEAG 545
Cdd:COG0318 404 ELRAFLRERLARYKVPRRVEfVDELPRTASGKIDRRALRERYAAGALEA 452
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
25-536 |
4.25e-140 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 415.11 E-value: 4.25e-140
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 25 FLKRAAQVYPQRDaVVY-----GARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVC 99
Cdd:cd12119 1 LLEHAARLHGDRE-IVSrthegEVHRYTYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVLHT 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 100 INIRLEARSIAFILRHCAARVLICDREFGAVAQQALAMLDA-PPLLVGIDDDQAERADLAHDLDYEAFLAQGDPGRPLsa 178
Cdd:cd12119 80 INPRLFPEQIAYIINHAEDRVVFVDRDFLPLLEAIAPRLPTvEHVVVMTDDAAMPEPAGVGVLAYEELLAAESPEYDW-- 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 179 PQ-NEWQSIAINYTSGTTGDPKGVVLHHRGAYLNA--CAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVcL 255
Cdd:cd12119 158 PDfDENTAAAICYTSGTTGNPKGVVYSHRSLVLHAmaALLTDGLGLSESDVVLPVVPMFHVNAWGLPYAAAMVGAKLV-L 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 256 --RKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVP--VSVITGGAAPPSAVIAAMEARGFSITHAYGMTES 331
Cdd:cd12119 237 pgPYLDPASLAELIEREGVTFAAGVPTVWQGLLDHLEANGRDLSslRRVVIGGSAVPRSLIEAFEERGVRVIHAWGMTET 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 332 YGPSTLCLWQPGVDELPLEARAQFMSRQGVAHPMLEeATVLDTDtGRPVPADGRTLGELVVRGNTVMKGYLHNPEATRAA 411
Cdd:cd12119 317 SPLGTVARPPSEHSNLSEDEQLALRAKQGRPVPGVE-LRIVDDD-GRELPWDGKAVGELQVRGPWVTKSYYKNDEESEAL 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 412 LADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAl 491
Cdd:cd12119 395 TEDGWLRTGDVATIDEDGYLTITDRSKDVIKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVLKEGA- 473
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 489250734 492 pRTTGDDLVRWCRERLAHFKAPRHVT-LTELPKTATGKIQKFVLRE 536
Cdd:cd12119 474 -TVTAEELLEFLADKVAKWWLPDDVVfVDEIPKTSTGKIDKKALRE 518
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
26-531 |
3.66e-132 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 391.97 E-value: 3.66e-132
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 26 LKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLE 105
Cdd:cd17631 1 LRRRARRHPDRTALVFGGRSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLT 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 106 ARSIAFILRHCAARVLIcdrefgavaqqalamldappllvgidddqaeradlaHDLdyeaflaqgdpgrplsapqnewqs 185
Cdd:cd17631 81 PPEVAYILADSGAKVLF------------------------------------DDL------------------------ 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 186 IAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTL-SGGTHVCLRKVQPDAIH 264
Cdd:cd17631 101 ALLMYTSGTTGRPKGAMLTHRNLLWNAVNALAALDLGPDDVLLVVAPLFHIGGLGVFTLPTLlRGGTVVILRKFDPETVL 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 265 AAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPV--SVITGGAAPPSAVIAAMEARGFSITHAYGMTESYGPSTLCLwqp 342
Cdd:cd17631 181 DLIERHRVTSFFLVPTMIQALLQHPRFATTDLSSlrAVIYGGAPMPERLLRALQARGVKFVQGYGMTETSPGVTFLS--- 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 343 gvdelPLEARAQFMSrQGVAHPMLEEAtVLDTDtGRPVPAdgRTLGELVVRGNTVMKGYLHNPEATRAALADGWLHTGDL 422
Cdd:cd17631 258 -----PEDHRRKLGS-AGRPVFFVEVR-IVDPD-GREVPP--GEVGEIVVRGPHVMAGYWNRPEATAAAFRDGWFHTGDL 327
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 423 AVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPrtTGDDLVRW 502
Cdd:cd17631 328 GRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGEAVVAVVVPRPGAEL--DEDELIAH 405
|
490 500 510
....*....|....*....|....*....|
gi 489250734 503 CRERLAHFKAPRHV-TLTELPKTATGKIQK 531
Cdd:cd17631 406 CRERLARYKIPKSVeFVDALPRNATGKILK 435
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
19-536 |
3.69e-126 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 379.08 E-value: 3.69e-126
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 19 PLSPLSFLKRAAQVYPQRdavVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLV 98
Cdd:cd05915 1 LERAAALFGRKEVVSRLH---TGEVHRTTYAEVYQRARRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLH 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 99 CINIRLEARSIAFILRHCAARVLICDREFGAVAQQALAmldappLLVGIDDDQAERADLAHdldYEAFLAQGDPGRPLSA 178
Cdd:cd05915 78 TANPRLSPKEIAYILNHAEDKVLLFDPNLLPLVEAIRG------ELKTVQHFVVMDEKAPE---GYLAYEEALGEEADPV 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 179 PQNEWQSIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLG--PRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLR 256
Cdd:cd05915 149 RVPERAACGMAYTTGTTGLPKGVVYSHRALVLHSLAASLVDGTAlsEKDVVLPVVPMFHVNAWCLPYAATLVGAKQVLPG 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 257 KVQPDAIH-AAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVP--VSVITGGAAPPSAVIAAMEARGFSITHAYGMTESYG 333
Cdd:cd05915 229 PRLDPASLvELFDGEGVTFTAGVPTVWLALADYLESTGHRLKtlRRLVVGGSAAPRSLIARFERMGVEVRQGYGLTETSP 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 334 PSTLCLWQPGVDELPLEARAQFMSRQGVAHpMLEEATVLDTDTgRPVPADGRTLGELVVRGNTVMKGYLHNPEATRA-AL 412
Cdd:cd05915 309 VVVQNFVKSHLESLSEEEKLTLKAKTGLPI-PLVRLRVADEEG-RPVPKDGKALGEVQLKGPWITGGYYGNEEATRSaLT 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 413 ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRaDALP 492
Cdd:cd05915 387 PDGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQERPLAVVVPR-GEKP 465
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 489250734 493 RTtgDDLVRWCRERLAHFKA-PRHVTLT-ELPKTATGKIQKFVLRE 536
Cdd:cd05915 466 TP--EELNEHLLKAGFAKWQlPDAYVFAeEIPRTSAGKFLKRALRE 509
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
26-536 |
2.23e-117 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 356.52 E-value: 2.23e-117
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 26 LKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLE 105
Cdd:PRK07656 11 LARAARRFGDKEAYVFGDQRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLNTRYT 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 106 ARSIAFILRHCAARVLICDREFGAVAQQALAMLDAPPLLVGIDDDQAERADlAHDLDYEAFLAQGDPGRPlsAPQNEWQS 185
Cdd:PRK07656 91 ADEAAYILARGDAKALFVLGLFLGVDYSATTRLPALEHVVICETEEDDPHT-EKMKTFTDFLAAGDPAER--APEVDPDD 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 186 IA-INYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTW-AVTLSGGTHVCLRKVQPDAI 263
Cdd:PRK07656 168 VAdILFTSGTTGRPKGAMLTHRQLLSNAADWAEYLGLTEGDRYLAANPFFHVFGYKAGVnAPLMRGATILPLPVFDPDEV 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 264 HAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPvSV---ITGGAAPPSAVIAAMEAR-GFS-ITHAYGMTESYGPSTLC 338
Cdd:PRK07656 248 FRLIETERITVLPGPPTMYNSLLQHPDRSAEDLS-SLrlaVTGAASMPVALLERFESElGVDiVLTGYGLSEASGVTTFN 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 339 lwQPGVDelpleaRAQFMSRQGVAHPMLEEATVldTDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAAL-ADGWL 417
Cdd:PRK07656 327 --RLDDD------RKTVAGTIGTAIAGVENKIV--NELGEEVPVG--EVGELLVRGPNVMKGYYDDPEATAAAIdADGWL 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 418 HTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAlpRTTGD 497
Cdd:PRK07656 395 HTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAAVIGVPDERLGEVGKAYVVLKPGA--ELTEE 472
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 489250734 498 DLVRWCRERLAHFKAPRHVT-LTELPKTATGKIQKFVLRE 536
Cdd:PRK07656 473 ELIAYCREHLAKYKVPRSIEfLDELPKNATGKVLKRALRE 512
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
23-535 |
1.72e-114 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 347.63 E-value: 1.72e-114
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 23 LSFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINI 102
Cdd:cd05936 2 ADLLEEAARRFPDKTALIFMGRKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPLNP 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 103 RLEARSIAFILRHCAARVLICDREFgavaQQALAMLDAPPLLVGIDDDqaeraDLAhdldyeaflaqgdpgrplsapqne 182
Cdd:cd05936 82 LYTPRELEHILNDSGAKALIVAVSF----TDLLAAGAPLGERVALTPE-----DVA------------------------ 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 183 wqsiAINYTSGTTGDPKGVVLHHRGAYLNA--CAGALIFQLGPRSIYLWTLPMFHCNGWshTWAVTLS---GGTHVCLRK 257
Cdd:cd05936 129 ----VLQYTSGTTGVPKGAMLTHRNLVANAlqIKAWLEDLLEGDDVVLAALPLFHVFGL--TVALLLPlalGATIVLIPR 202
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 258 VQPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHAS---VPAVPVSvITGGAAPPSAVIAAMEAR-GFSITHAYGMTESyG 333
Cdd:cd05936 203 FRPIGVLKEIRKHRVTIFPGVPTMYIALLNAPEFKkrdFSSLRLC-ISGGAPLPVEVAERFEELtGVPIVEGYGLTET-S 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 334 PSTLCLwqpgvdelPLEARAQFMSrqgVAHPMLE-EATVLDTDtGRPVPaDGRTlGELVVRGNTVMKGYLHNPEATRAAL 412
Cdd:cd05936 281 PVVAVN--------PLDGPRKPGS---IGIPLPGtEVKIVDDD-GEELP-PGEV-GELWVRGPQVMKGYWNRPEETAEAF 346
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 413 ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALP 492
Cdd:cd05936 347 VDGWLRTGDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAAVVGVPDPYSGEAVKAFVVLKEGASL 426
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 489250734 493 rtTGDDLVRWCRERLAHFKAPRHVT-LTELPKTATGKIQKFVLR 535
Cdd:cd05936 427 --TEEEIIAFCREQLAGYKVPRQVEfRDELPKSAVGKILRRELR 468
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
26-444 |
6.53e-106 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 323.88 E-value: 6.53e-106
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 26 LKRAAQVYPQRDAV-VYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRL 104
Cdd:pfam00501 1 LERQAARTPDKTALeVGEGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 105 EARSIAFILRHCAARVLICDREFGAVAQQALAMLDAPPLLVGIDDDQAEradLAHDLDYEAFLAQGDPGRPLSAPQnEWQ 184
Cdd:pfam00501 81 PAEELAYILEDSGAKVLITDDALKLEELLEALGKLEVVKLVLVLDRDPV---LKEEPLPEEAKPADVPPPPPPPPD-PDD 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 185 SIAINYTSGTTGDPKGVVLHHRGAYLNACAGALI----FQLGPRSIYLWTLPMFHCNGWSH-TWAVTLSGGTHVCLRKVQ 259
Cdd:pfam00501 157 LAYIIYTSGTTGKPKGVMLTHRNLVANVLSIKRVrprgFGLGPDDRVLSTLPLFHDFGLSLgLLGPLLAGATVVLPPGFP 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 260 ---PDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVP--VSVITGGAAPPSAVIAAMEAR-GFSITHAYGMTESYG 333
Cdd:pfam00501 237 aldPAALLELIERYKVTVLYGVPTLLNMLLEAGAPKRALLSslRLVLSGGAPLPPELARRFRELfGGALVNGYGLTETTG 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 334 PSTLCLWQPGVDELPlearaqfmSRQGVAHPmLEEATVLDTDTGRPVPADGRtlGELVVRGNTVMKGYLHNPEATRAAL- 412
Cdd:pfam00501 317 VVTTPLPLDEDLRSL--------GSVGRPLP-GTEVKIVDDETGEPVPPGEP--GELCVRGPGVMKGYLNDPELTAEAFd 385
|
410 420 430
....*....|....*....|....*....|..
gi 489250734 413 ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISG 444
Cdd:pfam00501 386 EDGWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
25-541 |
6.61e-103 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 320.60 E-value: 6.61e-103
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 25 FLKRAAQVYPQRDAVVYGAR--RYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINI 102
Cdd:PRK08315 21 LLDRTAARYPDREALVYRDQglRWTYREFNEEVDALAKGLLALGIEKGDRVGIWAPNVPEWVLTQFATAKIGAILVTINP 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 103 RLEARSIAFILRHCAARVLICDREFGAV-------------AQQALAMLDAPPL-----LVGIDDDQ----------AER 154
Cdd:PRK08315 101 AYRLSELEYALNQSGCKALIAADGFKDSdyvamlyelapelATCEPGQLQSARLpelrrVIFLGDEKhpgmlnfdelLAL 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 155 ADLAHDLDYEAFLAQGDPGRPlsapqnewqsIAINYTSGTTGDPKGVVLHHRGAYLNacaGALI---FQLGPRSIYLWTL 231
Cdd:PRK08315 181 GRAVDDAELAARQATLDPDDP----------INIQYTSGTTGFPKGATLTHRNILNN---GYFIgeaMKLTEEDRLCIPV 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 232 PMFHCNGWS-HTWAVTLSGGTHVC----------LRKVQpdaihaaiaEHAVTHLSAAPVV-MSMLIHAEHA----Svpa 295
Cdd:PRK08315 248 PLYHCFGMVlGNLACVTHGATMVYpgegfdplatLAAVE---------EERCTALYGVPTMfIAELDHPDFArfdlS--- 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 296 vpvSVITG---GAAPPS----AVIAAMEARGfsITHAYGMTESYGPSTlclwQPGVDElPLEARaqfMSRQGVAHPMLEe 368
Cdd:PRK08315 316 ---SLRTGimaGSPCPIevmkRVIDKMHMSE--VTIAYGMTETSPVST----QTRTDD-PLEKR---VTTVGRALPHLE- 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 369 ATVLDTDTGRPVPAdGRTlGELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGEN 447
Cdd:PRK08315 382 VKIVDPETGETVPR-GEQ-GELCTRGYSVMKGYWNDPEKTAEAIdADGWMHTGDLAVMDEEGYVNIVGRIKDMIIRGGEN 459
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 448 ISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAlpRTTGDDLVRWCRERLAHFKAPRHVT-LTELPKTAT 526
Cdd:PRK08315 460 IYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGA--TLTEEDVRDFCRGKIAHYKIPRYIRfVDEFPMTVT 537
|
570
....*....|....*
gi 489250734 527 GKIQKFVLREWARQE 541
Cdd:PRK08315 538 GKIQKFKMREMMIEE 552
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
188-530 |
1.06e-97 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 299.97 E-value: 1.06e-97
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 188 INYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPDAIHAAI 267
Cdd:cd04433 5 ILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVFLSTLPLFHIGGLFGLLGALLAGGTVVLLPKFDPEAALELI 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 268 AEHAVTHLSAAPVVMSMLIHAEHASVPAVP--VSVITGGAAPPSAVI-AAMEARGFSITHAYGMTESyGPSTLCLWQPGV 344
Cdd:cd04433 85 EREKVTILLGVPTLLARLLKAPESAGYDLSslRALVSGGAPLPPELLeRFEEAPGIKLVNGYGLTET-GGTVATGPPDDD 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 345 DELPLEAraqfmsrqGVAHPMLEeATVLDTDtGRPVPADGRtlGELVVRGNTVMKGYLHNPEATRAALADGWLHTGDLAV 424
Cdd:cd04433 164 ARKPGSV--------GRPVPGVE-VRIVDPD-GGELPPGEI--GELVVRGPSVMKGYWNNPEATAAVDEDGWYRTGDLGR 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 425 LHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPrtTGDDLVRWCR 504
Cdd:cd04433 232 LDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGADL--DAEELRAHVR 309
|
330 340
....*....|....*....|....*..
gi 489250734 505 ERLAHFKAPRHV-TLTELPKTATGKIQ 530
Cdd:cd04433 310 ERLAPYKVPRRVvFVDALPRTASGKID 336
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
25-536 |
1.41e-95 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 300.70 E-value: 1.41e-95
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 25 FLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRL 104
Cdd:PRK08316 16 ILRRSARRYPDKTALVFGDRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFML 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 105 EARSIAFILRHCAARVLICDREFGAVAQQALAmlDAPPLLVGIDDDQAERADLAHDLDYEAFLAQGDPGRPLSAPQNEwQ 184
Cdd:PRK08316 96 TGEELAYILDHSGARAFLVDPALAPTAEAALA--LLPVDTLILSLVLGGREAPGGWLDFADWAEAGSVAEPDVELADD-D 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 185 SIAINYTSGTTGDPKGVVLHHRgaylnacagALIFQ---------LGPRSIYLWTLPMFHCNGWsHTWAVT--LSGGTHV 253
Cdd:PRK08316 173 LAQILYTSGTESLPKGAMLTHR---------ALIAEyvscivagdMSADDIPLHALPLYHCAQL-DVFLGPylYVGATNV 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 254 CLRKVQPDAIHAAIAEHAVTHLSAAPVV-MSMLIHAEHAsvpAVPVSVIT----GGAAPPSAVIAAMEAR--GFSITHAY 326
Cdd:PRK08316 243 ILDAPDPELILRTIEAERITSFFAPPTVwISLLRHPDFD---TRDLSSLRkgyyGASIMPVEVLKELRERlpGLRFYNCY 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 327 GMTEsYGPSTLCLwqpGVDElplearaqFMSRQGVA-HPMLE-EATVLDtDTGRPVPADgrTLGELVVRGNTVMKGYLHN 404
Cdd:PRK08316 320 GQTE-IAPLATVL---GPEE--------HLRRPGSAgRPVLNvETRVVD-DDGNDVAPG--EVGEIVHRSPQLMLGYWDD 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 405 PEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFV 484
Cdd:PRK08316 385 PEKTAEAFRGGWFHSGDLGVMDEEGYITVVDRKKDMIKTGGENVASREVEEALYTHPAVAEVAVIGLPDPKWIEAVTAVV 464
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 489250734 485 ALRADAlpRTTGDDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKIQKFVLRE 536
Cdd:PRK08316 465 VPKAGA--TVTEDELIAHCRARLAGFKVPKRVIFVDeLPRNPSGKILKRELRE 515
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
25-546 |
2.50e-92 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 293.56 E-value: 2.50e-92
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 25 FLKRAAQVYPQRDAVVY-----GARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVC 99
Cdd:COG0365 14 CLDRHAEGRGDKVALIWegedgEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAMLACARIGAVHSP 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 100 INIRLEARSIAFILRHCAARVLICDREF---------GAVAQQALAMLDAPPLLVGIDDDQAErADLAHDLDYEAFLA-Q 169
Cdd:COG0365 94 VFPGFGAEALADRIEDAEAKVLITADGGlrggkvidlKEKVDEALEELPSLEHVIVVGRTGAD-VPMEGDLDWDELLAaA 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 170 GDPGRPLSAPQNEwqsIA-INYTSGTTGDPKGVVLHHRGAYLNA-CAGALIFQLGPRSIYLWTLPMfhcnGW--SHTWAV 245
Cdd:COG0365 173 SAEFEPEPTDADD---PLfILYTSGTTGKPKGVVHTHGGYLVHAaTTAKYVLDLKPGDVFWCTADI----GWatGHSYIV 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 246 T---LSGGTHVCLRKV----QPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVS-----VITGGAAPPSAVIA 313
Cdd:COG0365 246 YgplLNGATVVLYEGRpdfpDPGRLWELIEKYGVTVFFTAPTAIRALMKAGDEPLKKYDLSslrllGSAGEPLNPEVWEW 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 314 AMEARGFSITHAYGMTESYGPSTLCLwqPGvdelpLEARAQFMSRqgvahPMLE-EATVLDtDTGRPVPADgrTLGELVV 392
Cdd:COG0365 326 WYEAVGVPIVDGWGQTETGGIFISNL--PG-----LPVKPGSMGK-----PVPGyDVAVVD-EDGNPVPPG--EEGELVI 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 393 RGN--TVMKGYLHNPEATRAALAD---GWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAA 467
Cdd:COG0365 391 KGPwpGMFRGYWNDPERYRETYFGrfpGWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAA 470
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 468 VVARPDSRWGETPHAFVALRADALPR-TTGDDLVRWCRERLAHFKAPRHVT-LTELPKTATGKIQKFVLREWArQEEEAG 545
Cdd:COG0365 471 VVGVPDEIRGQVVKAFVVLKPGVEPSdELAKELQAHVREELGPYAYPREIEfVDELPKTRSGKIMRRLLRKIA-EGRPLG 549
|
.
gi 489250734 546 D 546
Cdd:COG0365 550 D 550
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
45-536 |
4.09e-92 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 292.38 E-value: 4.09e-92
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 45 RYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICD 124
Cdd:PRK07008 39 RYTYRDCERRAKQLAQALAALGVEPGDRVGTLAWNGYRHLEAYYGVSGSGAVCHTINPRLFPEQIAYIVNHAEDRYVLFD 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 125 REFGAVAQQALAMLDAPPLLVGIDDDQAERADLAHDLDYEAFLAQGDPGrpLSAPQ-NEWQSIAINYTSGTTGDPKGVVL 203
Cdd:PRK07008 119 LTFLPLVDALAPQCPNVKGWVAMTDAAHLPAGSTPLLCYETLVGAQDGD--YDWPRfDENQASSLCYTSGTTGNPKGALY 196
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 204 HHRGAYLNACAGAL--IFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCL-RKVQPDAIHAAIAEHAVTHLSAAPV 280
Cdd:PRK07008 197 SHRSTVLHAYGAALpdAMGLSARDAVLPVVPMFHVNAWGLPYSAPLTGAKLVLPgPDLDGKSLYELIEAERVTFSAGVPT 276
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 281 VMSMLI-HAEHASVPAVPV--SVITGGAAPPSAVIAAMEARGFSITHAYGMTESYGPSTLCLWQPGVDELPLEARAQFMS 357
Cdd:PRK07008 277 VWLGLLnHMREAGLRFSTLrrTVIGGSACPPAMIRTFEDEYGVEVIHAWGMTEMSPLGTLCKLKWKHSQLPLDEQRKLLE 356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 358 RQGVAHPMLEEATVldTDTGRPVPADGRTLGELVVRGNTVMKGYLHNPEAtraALADGWLHTGDLAVLHPDGYVEIKDRA 437
Cdd:PRK07008 357 KQGRVIYGVDMKIV--GDDGRELPWDGKAFGDLQVRGPWVIDRYFRGDAS---PLVDGWFPTGDVATIDADGFMQITDRS 431
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 438 KDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAlpRTTGDDLVRWCRERLAHFKAPRHVT 517
Cdd:PRK07008 432 KDVIKSGGEWISSIDIENVAVAHPAVAEAACIACAHPKWDERPLLVVVKRPGA--EVTREELLAFYEGKVAKWWIPDDVV 509
|
490 500
....*....|....*....|
gi 489250734 518 -LTELPKTATGKIQKFVLRE 536
Cdd:PRK07008 510 fVDAIPHTATGKLQKLKLRE 529
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
43-535 |
4.79e-91 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 285.72 E-value: 4.79e-91
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 43 ARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLI 122
Cdd:cd05934 1 GRRWTYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 123 CDrefgavaqqaLAMldappllvgidddqaeradlahdldyeaflaqgdpgrplsapqnewqsiaINYTSGTTGDPKGVV 202
Cdd:cd05934 81 VD----------PAS--------------------------------------------------ILYTSGTTGPPKGVV 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 203 LHHR----GAYLNACAGALifqlGPRSIYLWTLPMFHCNGWSHTWAVTLS-GGTHVCLRKVQPDAIHAAIAEHAVTHLSA 277
Cdd:cd05934 101 ITHAnltfAGYYSARRFGL----GEDDVYLTVLPLFHINAQAVSVLAALSvGATLVLLPRFSASRFWSDVRRYGATVTNY 176
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 278 APVVMSMLIHAEHASVP-AVPVSVItGGAAPPSAVIAAMEAR-GFSITHAYGMTESYGPSTLCLWQPGVDelplearaqf 355
Cdd:cd05934 177 LGAMLSYLLAQPPSPDDrAHRLRAA-YGAPNPPELHEEFEERfGVRLLEGYGMTETIVGVIGPRDEPRRP---------- 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 356 MSrQGVAHPmLEEATVLDtDTGRPVPADgrTLGELVVR---GNTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVE 432
Cdd:cd05934 246 GS-IGRPAP-GYEVRIVD-DDGQELPAG--EPGELVIRglrGWGFFKGYYNMPEATAEAMRNGWFHTGDLGYRDADGFFY 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 433 IKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAlpRTTGDDLVRWCRERLAHFKA 512
Cdd:cd05934 321 FVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGE--TLDPEELFAFCEGQLAYFKV 398
|
490 500
....*....|....*....|....
gi 489250734 513 PRHVTL-TELPKTATGKIQKFVLR 535
Cdd:cd05934 399 PRYIRFvDDLPKTPTEKVAKAQLR 422
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
19-536 |
2.15e-89 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 285.11 E-value: 2.15e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 19 PLSPLSFLKRAAQVYPQRDAV---VYGA-RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAG 94
Cdd:PRK06018 9 PLLCHRIIDHAARIHGNREVVtrsVEGPiVRTTYAQIHDRALKVSQALDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIG 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 95 AVLVCINIRLEARSIAFILRHCAARVLICDREFGAVAQqalAMLDAPP----LLVGIDDDQAERADLAHDLDYEAFLAQG 170
Cdd:PRK06018 89 AICHTVNPRLFPEQIAWIINHAEDRVVITDLTFVPILE---KIADKLPsverYVVLTDAAHMPQTTLKNAVAYEEWIAEA 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 171 DpGRPLSAPQNEWQSIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRS--IYLWTLPMFHCNGWSHTWAVTlS 248
Cdd:PRK06018 166 D-GDFAWKTFDENTAAGMCYTSGTTGDPKGVLYSHRSNVLHALMANNGDALGTSAadTMLPVVPLFHANSWGIAFSAP-S 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 249 GGTHVCLRKVQPDAIH--AAIAEHAVTHLSAAPVVMSMLIH---AEHASVPAVPVsVITGGAAPPSAVIAAMEARGFSIT 323
Cdd:PRK06018 244 MGTKLVMPGAKLDGASvyELLDTEKVTFTAGVPTVWLMLLQymeKEGLKLPHLKM-VVCGGSAMPRSMIKAFEDMGVEVR 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 324 HAYGMTESYGPSTLCLWQPGVDELPLEARAQFMSRQGVAhPMLEEATVLDtDTGRPVPADGRTLGELVVRGNTVMKGYLH 403
Cdd:PRK06018 323 HAWGMTEMSPLGTLAALKPPFSKLPGDARLDVLQKQGYP-PFGVEMKITD-DAGKELPWDGKTFGRLKVRGPAVAAAYYR 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 404 npEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAF 483
Cdd:PRK06018 401 --VDGEILDDDGFFDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERPLLI 478
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 489250734 484 VALRADALPrtTGDDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKIQKFVLRE 536
Cdd:PRK06018 479 VQLKPGETA--TREEILKYMDGKIAKWWMPDDVAFVDaIPHTATGKILKTALRE 530
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
26-541 |
3.51e-89 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 285.13 E-value: 3.51e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 26 LKRAAQVYPQRDAVVYG--ARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIR 103
Cdd:PRK12583 24 FDATVARFPDREALVVRhqALRYTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVNINPA 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 104 LEARSIAFILRHCAARVLICDREFGAvaQQALAMLDAppLLVGIDDDQAER-----------------ADLAHDLDYEAF 166
Cdd:PRK12583 104 YRASELEYALGQSGVRWVICADAFKT--SDYHAMLQE--LLPGLAEGQPGAlacerlpelrgvvslapAPPPGFLAWHEL 179
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 167 LAQGD--PGRPLSAPQNEWQ---SIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSH 241
Cdd:PRK12583 180 QARGEtvSREALAERQASLDrddPINIQYTSGTTGFPKGATLSHHNILNNGYFVAESLGLTEHDRLCVPVPLYHCFGMVL 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 242 TWAVTLSGGTHVCL--RKVQPDAIHAAIAEHAVTHLSAAPVV-MSMLIHAEHAS--VPAVPVSVITGGAAPPSAVIAAME 316
Cdd:PRK12583 260 ANLGCMTVGACLVYpnEAFDPLATLQAVEEERCTALYGVPTMfIAELDHPQRGNfdLSSLRTGIMAGAPCPIEVMRRVMD 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 317 ARGFS-ITHAYGMTESyGPSTLclwQPGVDElPLEARAQFMSRqgvAHPMLEeATVLDTDtGRPVPADgrTLGELVVRGN 395
Cdd:PRK12583 340 EMHMAeVQIAYGMTET-SPVSL---QTTAAD-DLERRVETVGR---TQPHLE-VKVVDPD-GATVPRG--EIGELCTRGY 407
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 396 TVMKGYLHNPEATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDS 474
Cdd:PRK12583 408 SVMKGYWNNPEATAESIdEDGWMHTGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTHPAVADVQVFGVPDE 487
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489250734 475 RWGETPHAFVALRADAlpRTTGDDLVRWCRERLAHFKAPRHVTLT-ELPKTATGKIQKFVLREWARQE 541
Cdd:PRK12583 488 KYGEEIVAWVRLHPGH--AASEEELREFCKARIAHFKVPRYFRFVdEFPMTVTGKVQKFRMREISIEE 553
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
25-539 |
1.30e-88 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 281.75 E-value: 1.30e-88
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 25 FLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASAL-ERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIR 103
Cdd:PRK06839 7 WIEKRAYLHPDRIAIITEEEEMTYKQLHEYVSKVAAYLiYELNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIR 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 104 LEARSIAFILRHCAARVLICDREFGAVAQ--QALAMLDAPPLLVGIDD-DQAERADLAhdldyeaflaqgdpgrplsaPQ 180
Cdd:PRK06839 87 LTENELIFQLKDSGTTVLFVEKTFQNMALsmQKVSYVQRVISITSLKEiEDRKIDNFV--------------------EK 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 181 NEWQSIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWS-HTWAVTLSGGTHVCLRKVQ 259
Cdd:PRK06839 147 NESASFIICYTSGTTGKPKGAVLTQENMFWNALNNTFAIDLTMHDRSIVLLPLFHIGGIGlFAFPTLFAGGVIIVPRKFE 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 260 PDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPvSV---ITGGAAPPSAVIAAMEARGFSITHAYGMTESyGPST 336
Cdd:PRK06839 227 PTKALSMIEKHKVTVVMGVPTIHQALINCSKFETTNLQ-SVrwfYNGGAPCPEELMREFIDRGFLFGQGFGMTET-SPTV 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 337 LCLwqpgvdelpleARAQFMSRQG-VAHP-MLEEATVLDTDTGRpVPADGrtLGELVVRGNTVMKGYLHNPEATRAALAD 414
Cdd:PRK06839 305 FML-----------SEEDARRKVGsIGKPvLFCDYELIDENKNK-VEVGE--VGELLIRGPNVMKEYWNRPDATEETIQD 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 415 GWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALprT 494
Cdd:PRK06839 371 GWLCTGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIPIAFIVKKSSSV--L 448
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 489250734 495 TGDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQKFVLREWAR 539
Cdd:PRK06839 449 IEKDVIEHCRLFLAKYKIPKEiVFLKELPKNATGKIQKAQLVNQLK 494
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
34-536 |
1.69e-87 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 278.81 E-value: 1.69e-87
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 34 PQRDAVVYGARR--YSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAF 111
Cdd:cd05926 1 PDAPALVVPGSTpaLTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEF 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 112 ILRHCAARVLICDREFGAVAQQALAMLDAPPLLVGIDDDQAERADLAHDLDYEAFLAQGDPGRPLSAPQNewqsIA-INY 190
Cdd:cd05926 81 YLADLGSKLVLTPKGELGPASRAASKLGLAILELALDVGVLIRAPSAESLSNLLADKKNAKSEGVPLPDD----LAlILH 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 191 TSGTTGDPKGVVLHHRgaylNACAGALI----FQLGPRSIYLWTLPMFHCNGWSHTWAVTL-SGGTHVCLRKVQPDAIHA 265
Cdd:cd05926 157 TSGTTGRPKGVPLTHR----NLAASATNitntYKLTPDDRTLVVMPLFHVHGLVASLLSTLaAGGSVVLPPRFSASTFWP 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 266 AIAEHAVTHLSAAPVVMSMLI--HAEHASVPAVPVSVI-TGGAAPPSAVIAAMEAR-GFSITHAYGMTESYGPSTLclwq 341
Cdd:cd05926 233 DVRDYNATWYTAVPTIHQILLnrPEPNPESPPPKLRFIrSCSASLPPAVLEALEATfGAPVLEAYGMTEAAHQMTS---- 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 342 pgvDELPLEARaQFMSrqgVAHPMLEEATVLDtDTGRPVPaDGRTlGELVVRGNTVMKGYLHNPEATRA-ALADGWLHTG 420
Cdd:cd05926 309 ---NPLPPGPR-KPGS---VGKPVGVEVRILD-EDGEILP-PGVV-GEICLRGPNVTRGYLNNPEANAEaAFKDGWFRTG 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 421 DLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAlpRTTGDDLV 500
Cdd:cd05926 379 DLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAVVLREGA--SVTEEELR 456
|
490 500 510
....*....|....*....|....*....|....*..
gi 489250734 501 RWCRERLAHFKAPRHVTLT-ELPKTATGKIQKFVLRE 536
Cdd:cd05926 457 AFCRKHLAAFKVPKKVYFVdELPKTATGKIQRRKVAE 493
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
17-549 |
2.08e-86 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 277.61 E-value: 2.08e-86
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 17 HVPLSPLSF-LKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERV-GVQAGERVAILAPNIPEMLEAHYGVPGAG 94
Cdd:PRK08314 6 TLPETSLFHnLEVSARRYPDKTAIVFYGRAISYRELLEEAERLAGYLQQEcGVRKGDRVLLYMQNSPQFVIAYYAILRAN 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 95 AVLVCINIRLEARSIAFILRHCAARVLICDREFGAVAQQALA--------------MLDAPPLLVGID--DDQAERADLA 158
Cdd:PRK08314 86 AVVVPVNPMNREEELAHYVTDSGARVAIVGSELAPKVAPAVGnlrlrhvivaqysdYLPAEPEIAVPAwlRAEPPLQALA 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 159 H--DLDYEAFLAQGDPGRPLSAPQNEwqsIA-INYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFH 235
Cdd:PRK08314 166 PggVVAWKEALAAGLAPPPHTAGPDD---LAvLPYTSGTTGVPKGCMHTHRTVMANAVGSVLWSNSTPESVVLAVLPLFH 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 236 CNGWSHTWAVTL-SGGTHVCLRKVQPDAIHAAIAEHAVTHLSAAPvvmSMLI---------HAEHASVpavpVSVITGGA 305
Cdd:PRK08314 243 VTGMVHSMNAPIyAGATVVLMPRWDREAAARLIERYRVTHWTNIP---TMVVdflaspglaERDLSSL----RYIGGGGA 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 306 APPSAVIAAMEAR-GFSITHAYGMTESYGPSTL---------CLWQP--GVDelplearaqfmsrqgvahpmleeATVLD 373
Cdd:PRK08314 316 AMPEAVAERLKELtGLDYVEGYGLTETMAQTHSnppdrpklqCLGIPtfGVD-----------------------ARVID 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 374 TDTGRPVPaDGRTlGELVVRGNTVMKGYLHNPEATRAALA--DG--WLHTGDLAVLHPDGYVEIKDRAKDIIISGGENIS 449
Cdd:PRK08314 373 PETLEELP-PGEV-GEIVVHGPQVFKGYWNRPEATAEAFIeiDGkrFFRTGDLGRMDEEGYFFITDRLKRMINASGFKVW 450
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 450 SLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPRTTGDDLVRWCRERLAHFKAPRHVTLTE-LPKTATGK 528
Cdd:PRK08314 451 PAEVENLLYKHPAIQEACVIATPDPRRGETVKAVVVLRPEARGKTTEEEIIAWAREHMAAYKYPRIVEFVDsLPKSGSGK 530
|
570 580
....*....|....*....|.
gi 489250734 529 IQKFVLrewarQEEEAGDAGR 549
Cdd:PRK08314 531 ILWRQL-----QEQEKARAAK 546
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
26-537 |
5.45e-86 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 274.53 E-value: 5.45e-86
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 26 LKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLE 105
Cdd:PRK03640 8 LKQRAFLTPDRTAIEFEEKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLS 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 106 ARSIAFILRHCAARVLICDREFgavaqqalamldAPPLLVGIDDDQAEradlahdldyeafLAQGDPGRPlsAPQNEWQ- 184
Cdd:PRK03640 88 REELLWQLDDAEVKCLITDDDF------------EAKLIPGISVKFAE-------------LMNGPKEEA--EIQEEFDl 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 185 -SIA-INYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPDA 262
Cdd:PRK03640 141 dEVAtIMYTSGTTGKPKGVIQTYGNHWWSAVGSALNLGLTEDDCWLAAVPIFHISGLSILMRSVIYGMRVVLVEKFDAEK 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 263 IHAAIAEHAVTHLSAAPVVMSMLIHA-EHASVPAVPVSVITGGAAPPSAVIAAMEARGFSITHAYGMTESygPSTLCLWQ 341
Cdd:PRK03640 221 INKLLQTGGVTIISVVSTMLQRLLERlGEGTYPSSFRCMLLGGGPAPKPLLEQCKEKGIPVYQSYGMTET--ASQIVTLS 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 342 PgvdelplearAQFMSRQGVAHPMLEEATVLDTDTGRPVPAdgRTLGELVVRGNTVMKGYLHNPEATRAALADGWLHTGD 421
Cdd:PRK03640 299 P----------EDALTKLGSAGKPLFPCELKIEKDGVVVPP--FEEGEIVVKGPNVTKGYLNREDATRETFQDGWFKTGD 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 422 LAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAlprtTGDDLVR 501
Cdd:PRK03640 367 IGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDKWGQVPVAFVVKSGEV----TEEELRH 442
|
490 500 510
....*....|....*....|....*....|....*..
gi 489250734 502 WCRERLAHFKAPRHVT-LTELPKTATGKIQKFVLREW 537
Cdd:PRK03640 443 FCEEKLAKYKVPKRFYfVEELPRNASGKLLRHELKQL 479
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
26-536 |
5.56e-82 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 265.87 E-value: 5.56e-82
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 26 LKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLE 105
Cdd:PRK07786 23 LARHALMQPDAPALRFLGNTTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLT 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 106 ARSIAFILRHCAARVLICDrefGAVAQQALAMLDAPPLLVGIDDDQAERADLAhdLDYEAFLAQ-GDPGRPLSAPQNewQ 184
Cdd:PRK07786 103 PPEIAFLVSDCGAHVVVTE---AALAPVATAVRDIVPLLSTVVVAGGSSDDSV--LGYEDLLAEaGPAHAPVDIPND--S 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 185 SIAINYTSGTTGDPKGVVLHHrgAYLNACAGALIFQLG---PRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVC--LRKVQ 259
Cdd:PRK07786 176 PALIMYTSGTTGRPKGAVLTH--ANLTGQAMTCLRTNGadiNSDVGFVGVPLFHIAGIGSMLPGLLLGAPTVIypLGAFD 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 260 PDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVSVITGGAAPPS-AVIAAMEAR--GFSITHAYGMTEsYGPST 336
Cdd:PRK07786 254 PGQLLDVLEAEKVTGIFLVPAQWQAVCAEQQARPRDLALRVLSWGAAPASdTLLRQMAATfpEAQILAAFGQTE-MSPVT 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 337 lCLwqpgvdelpLEARAQFMSRQGVAHPMLEEATVLDTDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAALADGW 416
Cdd:PRK07786 333 -CM---------LLGEDAIRKLGSVGKVIPTVAARVVDENMNDVPVG--EVGEIVYRAPTLMSGYWNNPEATAEAFAGGW 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 417 LHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPRTTg 496
Cdd:PRK07786 401 FHSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAAVRNDDAALTL- 479
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 489250734 497 DDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKIQKFVLRE 536
Cdd:PRK07786 480 EDLAEFLTDRLARYKHPKALEIVDaLPRNPAGKVLKTELRE 520
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
46-536 |
1.14e-81 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 261.13 E-value: 1.14e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 46 YSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRhcaarvlicdr 125
Cdd:cd05912 2 YTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLK----------- 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 126 efgavaqqalamldappllvgidddqaeraDLAHDLDyeaflaqgdpgrplsapqnewqSIA-INYTSGTTGDPKGVVLH 204
Cdd:cd05912 71 ------------------------------DSDVKLD----------------------DIAtIMYTSGTTGKPKGVQQT 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 205 HRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPDAIHAAIAEHAVTHLSAAPVVMSM 284
Cdd:cd05912 99 FGNHWWSAIGSALNLGLTEDDNWLCALPLFHISGLSILMRSVIYGMTVYLVDKFDAEQVLHLINSGKVTIISVVPTMLQR 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 285 LIHAEHASVPAVPVSVITGGAAPPSAVIAAMEARGFSITHAYGMTESYgpSTLCLWQPgvdelplearAQFMSRQG-VAH 363
Cdd:cd05912 179 LLEILGEGYPNNLRCILLGGGPAPKPLLEQCKEKGIPVYQSYGMTETC--SQIVTLSP----------EDALNKIGsAGK 246
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 364 PMLEEATVLDTDTGRPvpadgRTLGELVVRGNTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIIS 443
Cdd:cd05912 247 PLFPVELKIEDDGQPP-----YEVGEILLKGPNVTKGYLNRPDATEESFENGWFKTGDIGYLDEEGFLYVLDRRSDLIIS 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 444 GGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADalprTTGDDLVRWCRERLAHFKAPRHVT-LTELP 522
Cdd:cd05912 322 GGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKWGQVPVAFVVSERP----ISEEELIAYCSEKLAKYKVPKKIYfVDELP 397
|
490
....*....|....
gi 489250734 523 KTATGKIQKFVLRE 536
Cdd:cd05912 398 RTASGKLLRHELKQ 411
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
45-536 |
5.55e-81 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 260.01 E-value: 5.55e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 45 RYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICD 124
Cdd:cd05903 1 RLTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVVP 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 125 REFGAVAQQAlaMLDAPPLLVgidddqaeradlahdldyeaflaqgdpgrplsapqnewqsiainYTSGTTGDPKGVVLH 204
Cdd:cd05903 81 ERFRQFDPAA--MPDAVALLL--------------------------------------------FTSGTTGEPKGVMHS 114
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 205 HRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTW-AVTLSGGTHVCLRKVQPDAIHAAIAEHAVTHLSAAPVVMS 283
Cdd:cd05903 115 HNTLSASIRQYAERLGLGPGDVFLVASPMAHQTGFVYGFtLPLLLGAPVVLQDIWDPDKALALMREHGVTFMMGATPFLT 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 284 MLIHAEHASVPAVPV--SVITGGAAPPSAVIA-AMEARGFSITHAYGMTESYGPSTLClwqpgvdELPLEARaqFMSRQG 360
Cdd:cd05903 195 DLLNAVEEAGEPLSRlrTFVCGGATVPRSLARrAAELLGAKVCSAYGSTECPGAVTSI-------TPAPEDR--RLYTDG 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 361 VAHPMLEeATVLDtDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDI 440
Cdd:cd05903 266 RPLPGVE-IKVVD-DTGATLAPG--VEGELLSRGPSVFLGYLDRPDLTADAAPEGWFRTGDLARLDEDGYLRITGRSKDI 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 441 IISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPrtTGDDLVRWC-RERLAHFKAPRHVTLT 519
Cdd:cd05903 342 IIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGALL--TFDELVAYLdRQGVAKQYWPERLVHV 419
|
490
....*....|....*...
gi 489250734 520 E-LPKTATGKIQKFVLRE 536
Cdd:cd05903 420 DdLPRTPSGKVQKFRLRE 437
|
|
| menE |
TIGR01923 |
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, ... |
47-531 |
8.11e-79 |
|
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, which is involved in the fourth step of the menaquinone biosynthesis pathway. O-succinylbenzoate-CoA ligase, together with menB - naphtoate synthase, take 2-succinylbenzoate and convert it into 1,4-di-hydroxy-2- naphtoate. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone]
Pssm-ID: 162605 [Multi-domain] Cd Length: 436 Bit Score: 254.30 E-value: 8.11e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 47 SYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDRE 126
Cdd:TIGR01923 1 TWQDLDCEAAHLAKALKAQGIRSGSRVALVGQNSIEMVLLLHACLLLGAEIAMLNTRLTENERTNQLEDLDVQLLLTDSL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 127 FGAVAQQALAMldappllvgiddDQAERADLAhdlDYEaflaqgdpgrpLSAPQNEWQSIAINYTSGTTGDPKGVVLHHR 206
Cdd:TIGR01923 81 LEEKDFQADSL------------DRIEAAGRY---ETS-----------LSASFNMDQIATLMFTSGTTGKPKAVPHTFR 134
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 207 GAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGThvcLRKVQPDAIHAAI-AEHAVTHLSAAPVVMSML 285
Cdd:TIGR01923 135 NHYASAVGSKENLGFTEDDNWLLSLPLYHISGLSILFRWLIEGAT---LRIVDKFNQLLEMiANERVTHISLVPTQLNRL 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 286 IHAEHAsvPAVPVSVITGGAAPPSAVIAAMEARGFSITHAYGMTESYgpSTLCLWQPGVdelplearaqFMSRqgvahpm 365
Cdd:TIGR01923 212 LDEGGH--NENLRKILLGGSAIPAPLIEEAQQYGLPIYLSYGMTETC--SQVTTATPEM----------LHAR------- 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 366 leeatvldTDTGRPVP--------ADGRTLGELVVRGNTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDRA 437
Cdd:TIGR01923 271 --------PDVGRPLAgreikikvDNKEGHGEIMVKGANLMKGYLYQGELTPAFEQQGWFNTGDIGELDGEGFLYVLGRR 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 438 KDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADaLPRTTGDDlvrWCRERLAHFKAPRHVT 517
Cdd:TIGR01923 343 DDLIISGGENIYPEEIETVLYQHPGIQEAVVVPKPDAEWGQVPVAYIVSESD-ISQAKLIA---YLTEKLAKYKVPIAFE 418
|
490
....*....|....*
gi 489250734 518 -LTELPKTATGKIQK 531
Cdd:TIGR01923 419 kLDELPYNASGKILR 433
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
44-530 |
1.24e-78 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 255.22 E-value: 1.24e-78
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 44 RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLIC 123
Cdd:cd05911 9 KELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVIFT 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 124 DREFGAVAQQALAMLDAPPLLVGIDDDQAERADLAHDLDyeAFLAQGDPGRPLSAPQNEWQSIAINYTSGTTGDPKGVVL 203
Cdd:cd05911 89 DPDGLEKVKEAAKELGPKDKIIVLDDKPDGVLSIEDLLS--PTLGEEDEDLPPPLKDGKDDTAAILYSSGTTGLPKGVCL 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 204 HHRgaylNACAGALIFQL------GPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPDAIHAAIAEHAVTHLSA 277
Cdd:cd05911 167 SHR----NLIANLSQVQTflygndGSNDVILGFLPLYHIYGLFTTLASLLNGATVIIMPKFDSELFLDLIEKYKITFLYL 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 278 APVVMSMLihAEHASVPAV---PVSVITGGAAPPSA-VIAAMEARGFS--ITHAYGMTESygpSTLCLWQPGVDELPlea 351
Cdd:cd05911 243 VPPIAAAL--AKSPLLDKYdlsSLRVILSGGAPLSKeLQELLAKRFPNatIKQGYGMTET---GGILTVNPDGDDKP--- 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 352 raqfmsrqG-VAHPM-LEEATVLDTDTGRPVPADGRtlGELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGDLAVLHPD 428
Cdd:cd05911 315 --------GsVGRLLpNVEAKIVDDDGKDSLGPNEP--GEICVRGPQVMKGYYNNPEATKETFdEDGWLHTGDIGYFDED 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 429 GYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADalPRTTGDDLVRWCRERLA 508
Cdd:cd05911 385 GYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKPG--EKLTEKEVKDYVAKKVA 462
|
490 500
....*....|....*....|....
gi 489250734 509 HFKAPRH--VTLTELPKTATGKIQ 530
Cdd:cd05911 463 SYKQLRGgvVFVDEIPKSASGKIL 486
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
16-529 |
2.53e-77 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 252.54 E-value: 2.53e-77
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 16 NHVPLSPLSFLkrAAQVYPQRDAVVYGA--RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGA 93
Cdd:cd05904 3 TDLPLDSVSFL--FASAHPSRPALIDAAtgRALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 94 GAVLVCINIRLEARSIAFILRHCAARVLICDREFgaVAQqaLAMLDAPPLLVgiddDQAERADLAHDLDyeafLAQGDPG 173
Cdd:cd05904 81 GAVVTTANPLSTPAEIAKQVKDSGAKLAFTTAEL--AEK--LASLALPVVLL----DSAEFDSLSFSDL----LFEADEA 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 174 RPLSAPQNEWQSIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIF--QLGPRSIYLWTLPMFHCNGWSH-TWAVTLSGG 250
Cdd:cd05904 149 EPPVVVIKQDDVAALLYSSGTTGRSKGVMLTHRNLIAMVAQFVAGEgsNSDSEDVFLCVLPMFHIYGLSSfALGLLRLGA 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 251 THVCLRKVQPDAIHAAIAEHAVTHLSAAP-VVMSMLIHAEHASVPAVPVSVITGGAAPPSA-VIAAMEAR--GFSITHAY 326
Cdd:cd05904 229 TVVVMPRFDLEELLAAIERYKVTHLPVVPpIVLALVKSPIVDKYDLSSLRQIMSGAAPLGKeLIEAFRAKfpNVDLGQGY 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 327 GMTESYGPSTLCLwqpgvdeLPLEARAQFMSrQGVAHPMLEeATVLDTDTGRPVPAdGRTlGELVVRGNTVMKGYLHNPE 406
Cdd:cd05904 309 GMTESTGVVAMCF-------APEKDRAKYGS-VGRLVPNVE-AKIVDPETGESLPP-NQT-GELWIRGPSIMKGYLNNPE 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 407 ATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVA 485
Cdd:cd05904 378 ATAATIdKEGWLHTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAVIPYPDEEAGEVPMAFVV 457
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 489250734 486 LRADAlpRTTGDDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKI 529
Cdd:cd05904 458 RKPGS--SLTEDEIMDFVAKQVAPYKKVRKVAFVDaIPKSPSGKI 500
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
184-535 |
2.85e-76 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 244.88 E-value: 2.85e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 184 QSIAINYTSGTTGDPKGVVLHHRGAYLNAcagaliFQLGPRsiYLWT--------LPMFHCNGwshtwavtLSGGTHVCL 255
Cdd:cd05917 3 DVINIQFTSGTTGSPKGATLTHHNIVNNG------YFIGER--LGLTeqdrlcipVPLFHCFG--------SVLGVLACL 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 256 RKVQ----------PDAIHAAIAEHAVTHLSAAPvvmSMLIHA-EHASVPAVPVSV----ITGGAAPPSAVIAAM-EARG 319
Cdd:cd05917 67 THGAtmvfpspsfdPLAVLEAIEKEKCTALHGVP---TMFIAElEHPDFDKFDLSSlrtgIMAGAPCPPELMKRViEVMN 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 320 FS-ITHAYGMTESygpSTLCLwQPGVDElPLEARAQfmsrqGVAHPMLE-EATVLDTdTGRPVPADGRTlGELVVRGNTV 397
Cdd:cd05917 144 MKdVTIAYGMTET---SPVST-QTRTDD-SIEKRVN-----TVGRIMPHtEAKIVDP-EGGIVPPVGVP-GELCIRGYSV 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 398 MKGYLHNPEATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRW 476
Cdd:cd05917 212 MKGYWNDPEKTAEAIdGDGWLHTGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVPDERY 291
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 477 GETPHAFVALRADalPRTTGDDLVRWCRERLAHFKAPRHVT-LTELPKTATGKIQKFVLR 535
Cdd:cd05917 292 GEEVCAWIRLKEG--AELTEEDIKAYCKGKIAHYKVPRYVFfVDEFPLTVSGKIQKFKLR 349
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
24-540 |
5.40e-76 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 249.68 E-value: 5.40e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 24 SFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIR 103
Cdd:COG1021 29 DLLRRRAERHPDRIAVVDGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGAIPVFALPA 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 104 LEARSIAFILRHCAARVLICDREFGAVAQQALAM--LDAPPLL--VGIDDDQAERADLAHDLDyeaflAQGDPGRPLSAP 179
Cdd:COG1021 109 HRRAEISHFAEQSEAVAYIIPDRHRGFDYRALARelQAEVPSLrhVLVVGDAGEFTSLDALLA-----APADLSEPRPDP 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 180 QnewqSIAINYTS-GTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFH-----CNGwshTWAVTLSGGTHV 253
Cdd:COG1021 184 D----DVAFFQLSgGTTGLPKLIPRTHDDYLYSVRASAEICGLDADTVYLAALPAAHnfplsSPG---VLGVLYAGGTVV 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 254 CLRKVQPDAIHAAIAEHAVTHLSAAP-VVMSMLIHAEHAsvPAVPVS---VITGGAA-PPSAVIAAMEARGFSITHAYGM 328
Cdd:COG1021 257 LAPDPSPDTAFPLIERERVTVTALVPpLALLWLDAAERS--RYDLSSlrvLQVGGAKlSPELARRVRPALGCTLQQVFGM 334
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 329 TEsyGPstLCLWQPGVDElplEARaqfMSRQGvaHPM--LEEATVLDtDTGRPVPaDGRTlGELVVRGNTVMKGYLHNPE 406
Cdd:COG1021 335 AE--GL--VNYTRLDDPE---EVI---LTTQG--RPIspDDEVRIVD-EDGNPVP-PGEV-GELLTRGPYTIRGYYRAPE 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 407 ATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVA 485
Cdd:COG1021 400 HNARAFtPDGFYRTGDLVRRTPDGYLVVEGRAKDQINRGGEKIAAEEVENLLLAHPAVHDAAVVAMPDEYLGERSCAFVV 479
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 489250734 486 LRADALprtTGDDLVRWCRER-LAHFKAP-RHVTLTELPKTATGKIQKFVLREWARQ 540
Cdd:COG1021 480 PRGEPL---TLAELRRFLRERgLAAFKLPdRLEFVDALPLTAVGKIDKKALRAALAA 533
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
23-541 |
2.53e-74 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 245.73 E-value: 2.53e-74
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 23 LSFLKRAAQVYPQRDAVV------YGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAV 96
Cdd:PRK13295 27 NDDLDACVASCPDKTAVTavrlgtGAPRRFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGAV 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 97 LVCINIRLEARSIAFILRHCAARVLICDREFGAvaqqalamLDAPPLLVGIdddQAERADLAH--------DLDYEAFLA 168
Cdd:PRK13295 107 LNPLMPIFRERELSFMLKHAESKVLVVPKTFRG--------FDHAAMARRL---RPELPALRHvvvvggdgADSFEALLI 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 169 ------QGDPGRPLSAPQNEWQSIA-INYTSGTTGDPKGVVLHHRGAYLN--ACAGALifQLGPRSIYLWTLPMFHCNGW 239
Cdd:PRK13295 176 tpaweqEPDAPAILARLRPGPDDVTqLIYTSGTTGEPKGVMHTANTLMANivPYAERL--GLGADDVILMASPMAHQTGF 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 240 SHTWAVTLSGGTHVCLRKV-QPDAIHAAIAEHAVTH-LSAAPVVMSMLIHAEHA--SVPAVPVSVITGGAAPPSAVIAAM 315
Cdd:PRK13295 254 MYGLMMPVMLGATAVLQDIwDPARAAELIRTEGVTFtMASTPFLTDLTRAVKESgrPVSSLRTFLCAGAPIPGALVERAR 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 316 EARGFSITHAYGMTESYGPSTLCLWQPgvDELPLEaraqfmsRQGVAHPMLEeATVLDTDtGRPVPADgrTLGELVVRGN 395
Cdd:PRK13295 334 AALGAKIVSAWGMTENGAVTLTKLDDP--DERAST-------TDGCPLPGVE-VRVVDAD-GAPLPAG--QIGRLQVRGC 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 396 TVMKGYLHNPEATRAAlADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSR 475
Cdd:PRK13295 401 SNFGGYLKRPQLNGTD-ADGWFDTGDLARIDADGYIRISGRSKDVIIRGGENIPVVEIEALLYRHPAIAQVAIVAYPDER 479
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489250734 476 WGETPHAFVALRADAlpRTTGDDLVRWCRE-RLAHFKAP-RHVTLTELPKTATGKIQKFVLREWARQE 541
Cdd:PRK13295 480 LGERACAFVVPRPGQ--SLDFEEMVEFLKAqKVAKQYIPeRLVVRDALPRTPSGKIQKFRLREMLRGE 545
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
23-536 |
6.86e-72 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 239.90 E-value: 6.86e-72
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 23 LSFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINI 102
Cdd:PRK05605 35 VDLYDNAVARFGDRPALDFFGATTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAVVVEHNP 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 103 RLEARSIAFILRHCAARVLICDREFGAVAQQ-----------ALAMLDAPPLL------VGIDDDQAERADLA----HDL 161
Cdd:PRK05605 115 LYTAHELEHPFEDHGARVAIVWDKVAPTVERlrrttpletivSVNMIAAMPLLqrlalrLPIPALRKARAALTgpapGTV 194
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 162 DYEAFL--AQGDPGRPLSAPQNEWQSIA-INYTSGTTGDPKGVVLHHRGAYLNACAG-ALIFQLGPR-SIYLWTLPMFHC 236
Cdd:PRK05605 195 PWETLVdaAIGGDGSDVSHPRPTPDDVAlILYTSGTTGKPKGAQLTHRNLFANAAQGkAWVPGLGDGpERVLAALPMFHA 274
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 237 NGWshTWAVTLS---GGTHVCLRKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIH-AEHASVPAVPVSVITGGAA--PPSA 310
Cdd:PRK05605 275 YGL--TLCLTLAvsiGGELVLLPAPDIDLILDAMKKHPPTWLPGVPPLYEKIAEaAEERGVDLSGVRNAFSGAMalPVST 352
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 311 VIAAMEARGFSITHAYGMTESygpSTLCLWQPGVDElpleARAQFMsrqGVAHPmLEEATVLDTDTGRPVPADGrTLGEL 390
Cdd:PRK05605 353 VELWEKLTGGLLVEGYGLTET---SPIIVGNPMSDD----RRPGYV---GVPFP-DTEVRIVDPEDPDETMPDG-EEGEL 420
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 391 VVRGNTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVA 470
Cdd:PRK05605 421 LVRGPQVFKGYWNRPEETAKSFLDGWFRTGDVVVMEEDGFIRIVDRIKELIITGGFNVYPAEVEEVLREHPGVEDAAVVG 500
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 489250734 471 RPDSRWGETPHAFVALRADAlpRTTGDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQKFVLRE 536
Cdd:PRK05605 501 LPREDGSEEVVAAVVLEPGA--ALDPEGLRAYCREHLTRYKVPRRfYHVDELPRDQLGKVRRREVRE 565
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
26-536 |
4.24e-71 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 237.35 E-value: 4.24e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 26 LKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLE 105
Cdd:PRK06155 27 LARQAERYPDRPLLVFGGTRWTYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPINTALR 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 106 ARSIAFILRHCAARVLICDREFGAVAQQALAMLDAPPLLVGIDDDQAERADLAHDLdyeAFLAQGDPGRPLSAPQNEwQS 185
Cdd:PRK06155 107 GPQLEHILRNSGARLLVVEAALLAALEAADPGDLPLPAVWLLDAPASVSVPAGWST---APLPPLDAPAPAAAVQPG-DT 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 186 IAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPDA--I 263
Cdd:PRK06155 183 AAILYTSGTTGPSKGVCCPHAQFYWWGRNSAEDLEIGADDVLYTTLPLFHTNALNAFFQALLAGATYVLEPRFSASGfwP 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 264 HAAIAEHAVTHLSAAPVVMsMLIHAEHASVPAVPVSVITGGAAPPSAVIAAMEARGFSITHAYGMTESYGPStlclwqpG 343
Cdd:PRK06155 263 AVRRHGATVTYLLGAMVSI-LLSQPARESDRAHRVRVALGPGVPAALHAAFRERFGVDLLDGYGSTETNFVI-------A 334
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 344 VDelPLEARAQFMSRqgvAHPMLEeATVLDTDtGRPVPADgrTLGELVVRGN---TVMKGYLHNPEATRAALADGWLHTG 420
Cdd:PRK06155 335 VT--HGSQRPGSMGR---LAPGFE-ARVVDEH-DQELPDG--EPGELLLRADepfAFATGYFGMPEKTVEAWRNLWFHTG 405
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 421 DLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRadalPRTTGD--D 498
Cdd:PRK06155 406 DRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVPSELGEDEVMAAVVLR----DGTALEpvA 481
|
490 500 510
....*....|....*....|....*....|....*....
gi 489250734 499 LVRWCRERLAHFKAPRHVT-LTELPKTATGKIQKFVLRE 536
Cdd:PRK06155 482 LVRHCEPRLAYFAVPRYVEfVAALPKTENGKVQKFVLRE 520
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
30-537 |
5.71e-71 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 235.47 E-value: 5.71e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 30 AQVYPQRDAVV--YGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEAR 107
Cdd:PRK09088 5 ARLQPQRLAAVdlALGRRWTYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSAS 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 108 SIAFILRHCAarvlicdrefgavaqqalamldaPPLLVGIDDDQAERADlahDLDYEAFLAQGDPGRPLSAPQNEWQSIA 187
Cdd:PRK09088 85 ELDALLQDAE-----------------------PRLLLGDDAVAAGRTD---VEDLAAFIASADALEPADTPSIPPERVS 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 188 -INYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGW-SHTWAVTLSGGTHVCLRKVQPDAIHA 265
Cdd:PRK09088 139 lILFTSGTSGQPKGVMLSERNLQQTAHNFGVLGRVDAHSSFLCDAPMFHIIGLiTSVRPVLAVGGSILVSNGFEPKRTLG 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 266 --AIAEHAVTHLSAAPVVMSMLIH--AEHASVPAVPVSVITGGAAPPSAVIAAMEARGFSITHAYGMTES---YGPStlc 338
Cdd:PRK09088 219 rlGDPALGITHYFCVPQMAQAFRAqpGFDAAALRHLTALFTGGAPHAAEDILGWLDDGIPMVDGFGMSEAgtvFGMS--- 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 339 lwqpgVDELPLEARAqfmSRQGVAHPMLEeaTVLDTDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEAT-RAALADGWL 417
Cdd:PRK09088 296 -----VDCDVIRAKA---GAAGIPTPTVQ--TRVVDDQGNDCPAG--VPGELLLRGPNLSPGYWRRPQATaRAFTGDGWF 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 418 HTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALprTTGD 497
Cdd:PRK09088 364 RTGDIARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVPADGAP--LDLE 441
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 489250734 498 DLVRWCRERLAHFKAPRHVTLTE-LPKTATGKIQKFVLREW 537
Cdd:PRK09088 442 RIRSHLSTRLAKYKVPKHLRLVDaLPRTASGKLQKARLRDA 482
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
9-538 |
3.74e-70 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 236.78 E-value: 3.74e-70
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 9 ELAPNEANHVPLSPLSFLKRAAQVYPQRDAVVY--------GARRYSYRQLHERSCALASALERVGVQAGERVAILAPNI 80
Cdd:PRK07529 14 EAVPLAARDLPASTYELLSRAAARHPDAPALSFlldadpldRPETWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNL 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 81 PEMLEAHYGvpGAGAVLVC-INIRLEARSIAFILRHCAARVLICDREFGA--VAQQALAMLDAPP-----LLVGIDDDQA 152
Cdd:PRK07529 94 PETHFALWG--GEAAGIANpINPLLEPEQIAELLRAAGAKVLVTLGPFPGtdIWQKVAEVLAALPelrtvVEVDLARYLP 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 153 ERADLAH----------DLDYEAFLAQGDPGRPLSAPQNEWQSIAINY-TSGTTGDPKGVVLHHRGAYLNACAGALIFQL 221
Cdd:PRK07529 172 GPKRLAVplirrkaharILDFDAELARQPGDRLFSGRPIGPDDVAAYFhTGGTTGMPKLAQHTHGNEVANAWLGALLLGL 251
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 222 GPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQP-------DAIHAAIAEHAVTHLSAAPVVMSMLihaehASVP 294
Cdd:PRK07529 252 GPGDTVFCGLPLFHVNALLVTGLAPLARGAHVVLATPQGyrgpgviANFWKIVERYRINFLSGVPTVYAAL-----LQVP 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 295 --AVPVS----VITGGAAPPSAVIAAMEAR-GFSITHAYGMTES---------YGPStlclwQPGVDELPL---EARAqf 355
Cdd:PRK07529 327 vdGHDISslryALCGAAPLPVEVFRRFEAAtGVRIVEGYGLTEAtcvssvnppDGER-----RIGSVGLRLpyqRVRV-- 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 356 msrqgvahpmleeaTVLDTDTGRPVPADGRTLGELVVRGNTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKD 435
Cdd:PRK07529 400 --------------VILDDAGRYLRDCAVDEVGVLCIAGPNVFSGYLEAAHNKGLWLEDGWLNTGDLGRIDADGYFWLTG 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 436 RAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAlpRTTGDDLVRWCRERLAHFKA-PR 514
Cdd:PRK07529 466 RAKDLIIRGGHNIDPAAIEEALLRHPAVALAAAVGRPDAHAGELPVAYVQLKPGA--SATEAELLAFARDHIAERAAvPK 543
|
570 580
....*....|....*....|....*
gi 489250734 515 HVT-LTELPKTATGKIQKFVLREWA 538
Cdd:PRK07529 544 HVRiLDALPKTAVGKIFKPALRRDA 568
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
24-536 |
6.67e-70 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 232.85 E-value: 6.67e-70
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 24 SFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIR 103
Cdd:PRK06145 6 ASIAFHARRTPDRAALVYRDQEISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPINYR 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 104 LEARSIAFILRHCAARVLICDREFgavaqQALAMLDAPPLLVgiddDQAERADLAHdldyeafLAQGDPGRPLSAPQNEW 183
Cdd:PRK06145 86 LAADEVAYILGDAGAKLLLVDEEF-----DAIVALETPKIVI----DAAAQADSRR-------LAQGGLEIPPQAAVAPT 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 184 QSIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWS-HTWAVTLSGGTHVCLRKVQPDA 262
Cdd:PRK06145 150 DLVRLMYTSGTTDRPKGVMHSYGNLHWKSIDHVIALGLTASERLLVVGPLYHVGAFDlPGIAVLWVGGTLRIHREFDPEA 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 263 IHAAIAEHAVTHLSAAPVVMSMLIhaehasvpAVPVS----------VITGGAAPPSAVIAAMEA--RGFSITHAYGMTE 330
Cdd:PRK06145 230 VLAAIERHRLTCAWMAPVMLSRVL--------TVPDRdrfdldslawCIGGGEKTPESRIRDFTRvfTRARYIDAYGLTE 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 331 SYGPSTLCLWQPGVDELPLEARAqfmsrqgVAHPMLEeatvLDTDTGRPVPADGRtlGELVVRGNTVMKGYLHNPEATRA 410
Cdd:PRK06145 302 TCSGDTLMEAGREIEKIGSTGRA-------LAHVEIR----IADGAGRWLPPNMK--GEICMRGPKVTKGYWKDPEKTAE 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 411 ALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADA 490
Cdd:PRK06145 369 AFYGDWFRSGDVGYLDEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRWGERITAVVVLNPGA 448
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 489250734 491 lpRTTGDDLVRWCRERLAHFKAPRHVTL-TELPKTATGKIQKFVLRE 536
Cdd:PRK06145 449 --TLTLEALDRHCRQRLASFKVPRQLKVrDELPRNPSGKVLKRVLRD 493
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
22-542 |
1.51e-69 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 233.78 E-value: 1.51e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 22 PLS-FLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCI 100
Cdd:PRK06178 34 PLTeYLRAWARERPQRPAIIFYGHVITYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPV 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 101 NIRLEARSIAFILRHCAARVLICDREFGAVAQQALA--------------MLDAPPLLVGIDDDQAER--ADLAHDLdye 164
Cdd:PRK06178 114 SPLFREHELSYELNDAGAEVLLALDQLAPVVEQVRAetslrhvivtsladVLPAEPTLPLPDSLRAPRlaAAGAIDL--- 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 165 aFLAQGDPGRPLSAPQNEWQSIA-INYTSGTTGDPKGVVLHHRG-AYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHT 242
Cdd:PRK06178 191 -LPALRACTAPVPLPPPALDALAaLNYTGGTTGMPKGCEHTQRDmVYTAAAAYAVAVVGGEDSVFLSFLPEFWIAGENFG 269
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 243 WAVTL-SGGTHVCLRKVQPDAIHAAIAEHAVTHlsaapvvMSMLIHA-----EHASVPAVPVSVITggAAPPSAVIAAM- 315
Cdd:PRK06178 270 LLFPLfSGATLVLLARWDAVAFMAAVERYRVTR-------TVMLVDNavelmDHPRFAEYDLSSLR--QVRVVSFVKKLn 340
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 316 --------EARGFSITHA-YGMTESYGPSTLCLWQPgVDELPLEARAQFMsrqGVAHPMlEEATVLDTDTGRPVPADGRt 386
Cdd:PRK06178 341 pdyrqrwrALTGSVLAEAaWGMTETHTCDTFTAGFQ-DDDFDLLSQPVFV---GLPVPG-TEFKICDFETGELLPLGAE- 414
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 387 lGELVVRGNTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEA 466
Cdd:PRK06178 415 -GEIVVRTPSLLKGYWNKPEATAEALRDGWLHTGDIGKIDEQGFLHYLGRRKEMLKVNGMSVFPSEVEALLGQHPAVLGS 493
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 489250734 467 AVVARPDSRWGETPHAFVALRADAlpRTTGDDLVRWCRERLAHFKAPRHVTLTELPKTATGKIQKFVLREWARQEE 542
Cdd:PRK06178 494 AVVGRPDPDKGQVPVAFVQLKPGA--DLTAAALQAWCRENMAVYKVPEIRIVDALPMTATGKVRKQDLQALAEELK 567
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
28-539 |
1.68e-69 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 233.10 E-value: 1.68e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 28 RAAQVYPQRDAVV--YGARrYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLE 105
Cdd:PRK06087 31 QTARAMPDKIAVVdnHGAS-YTYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVPLLPSWR 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 106 ARSIAFILRHCAARVLICDREFGAV--AQQALAMLDAPPLL---VGIDDDQAERADLAhdldYEAFLAQGDP-GRPLSAP 179
Cdd:PRK06087 110 EAELVWVLNKCQAKMFFAPTLFKQTrpVDLILPLQNQLPQLqqiVGVDKLAPATSSLS----LSQIIADYEPlTTAITTH 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 180 QNEWQsiAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSH-TWAVTLSGGTHVCLRKV 258
Cdd:PRK06087 186 GDELA--AVLFTSGTEGLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHgVTAPFLIGARSVLLDIF 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 259 QPDAIHAAIAEHAVT-HLSAAPVVMSML--IHAEHASVPAVPVsVITGGAAPPSAVIAAMEARGFSITHAYGMTES---- 331
Cdd:PRK06087 264 TPDACLALLEQQRCTcMLGATPFIYDLLnlLEKQPADLSALRF-FLCGGTTIPKKVARECQQRGIKLLSVYGSTESspha 342
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 332 YGPSTLCL-WQPGVDELPLEaraqfmsrqGVahpmleEATVLDTDTgRPVPADgrTLGELVVRGNTVMKGYLHNPEATRA 410
Cdd:PRK06087 343 VVNLDDPLsRFMHTDGYAAA---------GV------EIKVVDEAR-KTLPPG--CEGEEASRGPNVFMGYLDEPELTAR 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 411 AL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRAD 489
Cdd:PRK06087 405 ALdEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAP 484
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 489250734 490 ALPRTTGDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQKFVLR-EWAR 539
Cdd:PRK06087 485 HHSLTLEEVVAFFSRKRVAKYKYPEHiVVIDKLPRTASGKIQKFLLRkDIMR 536
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
25-546 |
2.54e-69 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 232.24 E-value: 2.54e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 25 FLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRL 104
Cdd:PRK07470 12 FLRQAARRFPDRIALVWGDRSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVWVPTNFRQ 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 105 EARSIAFILRHCAARVLICDREFgavAQQALAMLDAPPLL---VGIDDDQAEradlahdLDYEAFLAQgDPGRPLSAPQN 181
Cdd:PRK07470 92 TPDEVAYLAEASGARAMICHADF---PEHAAAVRAASPDLthvVAIGGARAG-------LDYEALVAR-HLGARVANAAV 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 182 EW-QSIAINYTSGTTGDPKGVVL-HHRGAY-LNACAGALIFQLGPRSIYLWTLPMFHCNGwSHTWAVTLSGGTHVCL--R 256
Cdd:PRK07470 161 DHdDPCWFFFTSGTTGRPKAAVLtHGQMAFvITNHLADLMPGTTEQDASLVVAPLSHGAG-IHQLCQVARGAATVLLpsE 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 257 KVQPDAIHAAIAEHAVTHLSAAPVVMSMLIhaEHASVPAVPVS----VITGGAAPPSA-VIAAMEARGFSITHAYGMTES 331
Cdd:PRK07470 240 RFDPAEVWALVERHRVTNLFTVPTILKMLV--EHPAVDRYDHSslryVIYAGAPMYRAdQKRALAKLGKVLVQYFGLGEV 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 332 YGPSTLClwQPGVDELPLEARAQF----MSRQGVahpmleEATVLDtDTGRPVPAdGRTlGELVVRGNTVMKGYLHNPEA 407
Cdd:PRK07470 318 TGNITVL--PPALHDAEDGPDARIgtcgFERTGM------EVQIQD-DEGRELPP-GET-GEICVIGPAVFAGYYNNPEA 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 408 TRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALR 487
Cdd:PRK07470 387 NAKAFRDGWFRTGDLGHLDARGFLYITGRASDMYISGGSNVYPREIEEKLLTHPAVSEVAVLGVPDPVWGEVGVAVCVAR 466
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 488 ADALPrtTGDDLVRWCRERLAHFKAPRHVTL-TELPKTATGKIQKFVLREwarQEEEAGD 546
Cdd:PRK07470 467 DGAPV--DEAELLAWLDGKVARYKLPKRFFFwDALPKSGYGKITKKMVRE---ELEERGL 521
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
26-506 |
4.21e-69 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 233.45 E-value: 4.21e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 26 LKRAAQVYPQRDAVVY----GARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCIN 101
Cdd:COG1022 17 LRRRAARFPDRVALREkedgIWQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAVTVPIY 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 102 IRLEARSIAFILRHCAARVLICDREfgAVAQQALAMLDAPPLL---VGIDDDQAERADLAHDLDyeAFLAQGDPGRPLSA 178
Cdd:COG1022 97 PTSSAEEVAYILNDSGAKVLFVEDQ--EQLDKLLEVRDELPSLrhiVVLDPRGLRDDPRLLSLD--ELLALGREVADPAE 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 179 PQNEWQSIA------INYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGwsHTWAVT-LSGGT 251
Cdd:COG1022 173 LEARRAAVKpddlatIIYTSGTTGRPKGVMLTHRNLLSNARALLERLPLGPGDRTLSFLPLAHVFE--RTVSYYaLAAGA 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 252 HVC-----------LRKVQPdaihaaiaehavTHLSAAP-----VVMSMLIHAEHASVP-------AVPVSV-------- 300
Cdd:COG1022 251 TVAfaespdtlaedLREVKP------------TFMLAVPrvwekVYAGIQAKAEEAGGLkrklfrwALAVGRryararla 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 301 ----------------------------------ITGGAAPPSAVIAAMEARGFSITHAYGMTESYGPSTlcLWQPGvde 346
Cdd:COG1022 319 gkspslllrlkhaladklvfsklrealggrlrfaVSGGAALGPELARFFRALGIPVLEGYGLTETSPVIT--VNRPG--- 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 347 lplearaqfmsrqgvaHPMLEeaTVldtdtGRPVP-------ADgrtlGELVVRGNTVMKGYLHNPEATRAAL-ADGWLH 418
Cdd:COG1022 394 ----------------DNRIG--TV-----GPPLPgvevkiaED----GEILVRGPNVMKGYYKNPEATAEAFdADGWLH 446
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 419 TGDLAVLHPDGYVEIKDRAKDIII-SGGENISSLEIEEVLYQHPEVVEAAVVArpDSRwgetPH--AFVALRADALPrtt 495
Cdd:COG1022 447 TGDIGELDEDGFLRITGRKKDLIVtSGGKNVAPQPIENALKASPLIEQAVVVG--DGR----PFlaALIVPDFEALG--- 517
|
570
....*....|.
gi 489250734 496 gddlvRWCRER 506
Cdd:COG1022 518 -----EWAEEN 523
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
17-536 |
8.08e-69 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 230.64 E-value: 8.08e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 17 HVPLSPLSFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHygvpGAGAV 96
Cdd:PRK06188 9 HSGATYGHLLVSALKRYPDRPALVLGDTRLTYGQLADRISRYIQAFEALGLGTGDAVALLSLNRPEVLMAI----GAAQL 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 97 LVCINIRLEARSI----AFILRHCAARVLICDRefGAVAQQALAMLD-APPL--LVGIDDdqaerADLAHDLDYEAflAQ 169
Cdd:PRK06188 85 AGLRRTALHPLGSlddhAYVLEDAGISTLIVDP--APFVERALALLArVPSLkhVLTLGP-----VPDGVDLLAAA--AK 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 170 GDPgRPLSAPQNEWQSIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGwSHTWAVTLSG 249
Cdd:PRK06188 156 FGP-APLVAAALPPDIAGLAYTGGTTGKPKGVMGTHRSIATMAQIQLAEWEWPADPRFLMCTPLSHAGG-AFFLPTLLRG 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 250 GTHVCLRKVQPDAIHAAIAEHAVThlsAAPVVMSMlIHA--EHASVPAVPVS---VITGGAAP--PSAVIAAMEARGFSI 322
Cdd:PRK06188 234 GTVIVLAKFDPAEVLRAIEEQRIT---ATFLVPTM-IYAllDHPDLRTRDLSsleTVYYGASPmsPVRLAEAIERFGPIF 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 323 THAYGMTESygPSTLCLWQPGvDELPleARAQFMSRQGVAHPMLEEAtVLDTDtGRPVPADgrTLGELVVRGNTVMKGYL 402
Cdd:PRK06188 310 AQYYGQTEA--PMVITYLRKR-DHDP--DDPKRLTSCGRPTPGLRVA-LLDED-GREVAQG--EVGEICVRGPLVMDGYW 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 403 HNPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHA 482
Cdd:PRK06188 381 NRPEETAEAFRDGWLHTGDVAREDEDGFYYIVDRKKDMIVTGGFNVFPREVEDVLAEHPAVAQVAVIGVPDEKWGEAVTA 460
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 489250734 483 FVALRADALPRTTgdDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKIQKFVLRE 536
Cdd:PRK06188 461 VVVLRPGAAVDAA--ELQAHVKERKGSVHAPKQVDFVDsLPLTALGKPDKKALRA 513
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
38-541 |
3.54e-68 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 228.25 E-value: 3.54e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 38 AVVYGA-RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHC 116
Cdd:PRK08276 3 VIMAPSgEVVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDS 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 117 AARVLICDREFGAVAQQALAMLDAPPLLVGIDDDQAERADlahdlDYEAFLAqgdpGRPLSAPQNEWQSIAINYTSGTTG 196
Cdd:PRK08276 83 GAKVLIVSAALADTAAELAAELPAGVPLLLVVAGPVPGFR-----SYEEALA----AQPDTPIADETAGADMLYSSGTTG 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 197 DPKGVVLHHRGAYLNACAGALIFQLG------PRSIYLWTLPMFHC--NGWSHTwaVTLSGGTHVCLRKVQPDAIHAAIA 268
Cdd:PRK08276 154 RPKGIKRPLPGLDPDEAPGMMLALLGfgmyggPDSVYLSPAPLYHTapLRFGMS--ALALGGTVVVMEKFDAEEALALIE 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 269 EHAVTHLSAAP---VVMSMLIHAEHASVPAVPVSVITGGAAP-PSAVIAAM-EARGFSITHAYGMTESYGpSTLClwqpg 343
Cdd:PRK08276 232 RYRVTHSQLVPtmfVRMLKLPEEVRARYDVSSLRVAIHAAAPcPVEVKRAMiDWWGPIIHEYYASSEGGG-VTVI----- 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 344 vdelpleARAQFMSRQG-VAHPMLEEATVLDtDTGRPVPAdgRTLGELVVRGNTVMKGYLHNPEATRAA-LADGWLHTGD 421
Cdd:PRK08276 306 -------TSEDWLAHPGsVGKAVLGEVRILD-EDGNELPP--GEIGTVYFEMDGYPFEYHNDPEKTAAArNPHGWVTVGD 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 422 LAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPRT-TGDDLV 500
Cdd:PRK08276 376 VGYLDEDGYLYLTDRKSDMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMGERVKAVVQPADGADAGDaLAAELI 455
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 489250734 501 RWCRERLAHFKAPRHVTLT-ELPKTATGKIQKFVLRE--WARQE 541
Cdd:PRK08276 456 AWLRGRLAHYKCPRSIDFEdELPRTPTGKLYKRRLRDryWEGRQ 499
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
45-529 |
4.10e-67 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 223.51 E-value: 4.10e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 45 RYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLIcd 124
Cdd:cd05935 1 SLTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAV-- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 125 refgavaqqALAMLDappllvgidddqaeraDLAhdldyeaflaqgdpgrplsapqnewqsiAINYTSGTTGDPKGVVLH 204
Cdd:cd05935 79 ---------VGSELD----------------DLA----------------------------LIPYTSGTTGLPKGCMHT 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 205 HRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTW-AVTLSGGTHVCLRKVQPDAIHAAIAEHAVTHLSAAP-VVM 282
Cdd:cd05935 106 HFSAAANALQSAVWTGLTPSDVILACLPLFHVTGFVGSLnTAVYVGGTYVLMARWDRETALELIEKYKVTFWTNIPtMLV 185
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 283 SMLIHAEHASVPAVPVSVITGGAAP-PSAVIAAMEAR-GFSITHAYGMTESYGPSTLclwQPgvdelPLEARAQFmsrQG 360
Cdd:cd05935 186 DLLATPEFKTRDLSSLKVLTGGGAPmPPAVAEKLLKLtGLRFVEGYGLTETMSQTHT---NP-----PLRPKLQC---LG 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 361 VahPMLE-EATVLDTDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAALA--DG--WLHTGDLAVLHPDGYVEIKD 435
Cdd:cd05935 255 I--P*FGvDARVIDIETGRELPPN--EVGEIVVRGPQIFKGYWNRPEETEESFIeiKGrrFFRTGDLGYMDEEGYFFFVD 330
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 436 RAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPRTTGDDLVRWCRERLAHFKAPRH 515
Cdd:cd05935 331 RVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLRPEYRGKVTEEDIIEWAREQMAAYKYPRE 410
|
490
....*....|....*
gi 489250734 516 VTLT-ELPKTATGKI 529
Cdd:cd05935 411 VEFVdELPRSASGKI 425
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
35-536 |
1.30e-66 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 222.55 E-value: 1.30e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 35 QRDAVVYGARRYSYRQLHERSCALASAL-ERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSiafiL 113
Cdd:cd05941 1 DRIAIVDDGDSITYADLVARAARLANRLlALGKDLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAE----L 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 114 RHcaarvLICDREFGAVAQQALamldappllvgidddqaeradlahdldyeaflaqgdpgrplsapqnewqsiaINYTSG 193
Cdd:cd05941 77 EY-----VITDSEPSLVLDPAL----------------------------------------------------ILYTSG 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 194 TTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTL-SGGTHVCLRKVQPDAIHAAIAEHAV 272
Cdd:cd05941 100 TTGRPKGVVLTHANLAANVRALVDAWRWTEDDVLLHVLPLHHVHGLVNALLCPLfAGASVEFLPKFDPKEVAISRLMPSI 179
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 273 THLSAAPVVMSMLI--HAEHASVPAVPVSV--------ITGGAAPPSAVIAAMEAR-GFSITHAYGMTESyGPSTLClwq 341
Cdd:cd05941 180 TVFMGVPTIYTRLLqyYEAHFTDPQFARAAaaerlrlmVSGSAALPVPTLEEWEAItGHTLLERYGMTEI-GMALSN--- 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 342 pgvdelPL--EARAQFMsrqGVAHPMLeEATVLDTDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAAL-ADGWLH 418
Cdd:cd05941 256 ------PLdgERRPGTV---GMPLPGV-QARIVDEETGEPLPRG--EVGEIQVRGPSVFKEYWNKPEATKEEFtDDGWFK 323
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 419 TGDLAVLHPDGYVEIKDRAK-DIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPRTTgD 497
Cdd:cd05941 324 TGDLGVVDEDGYYWILGRSSvDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVVVLRAGAAALSL-E 402
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 489250734 498 DLVRWCRERLAHFKAPRHVTL-TELPKTATGKIQKFVLRE 536
Cdd:cd05941 403 ELKEWAKQRLAPYKRPRRLILvDELPRNAMGKVNKKELRK 442
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
18-542 |
2.42e-66 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 225.43 E-value: 2.42e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 18 VPLSPLSFLKRAAQVYPQRDAVVYG---ARRYSYRQLHERSCALASAL-ERVGVQAGERVAILAPNIPEMLEAHYGVPGA 93
Cdd:PRK05620 8 VPLSLTRILEYGSTVHGDTTVTTWGgaeQEQTTFAAIGARAAALAHALhDELGITGDQRVGSMMYNCAEHLEVLFAVACM 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 94 GAVLVCINIRLEARSIAFILRHCAARVLICDRefgAVAQQALAMLDAPP-----LLVGIDDDQAERADLA---HDLDYEA 165
Cdd:PRK05620 88 GAVFNPLNKQLMNDQIVHIINHAEDEVIVADP---RLAEQLGEILKECPcvravVFIGPSDADSAAAHMPegiKVYSYEA 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 166 FLaqgdPGRPLSAP---QNEWQSIAINYTSGTTGDPKGVVLHHRGAYLNACA--GALIFQLGPRSIYLWTLPMFHCNGWS 240
Cdd:PRK05620 165 LL----DGRSTVYDwpeLDETTAAAICYSTGTTGAPKGVVYSHRSLYLQSLSlrTTDSLAVTHGESFLCCVPIYHVLSWG 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 241 HTWAVTLSGGTHVCL-RKVQPDAIHAAIAEHAVTHLSAAPVV-MSMLIHAEHASVPAVPVSVI-TGGAAPPSAVIAAMEA 317
Cdd:PRK05620 241 VPLAAFMSGTPLVFPgPDLSAPTLAKIIATAMPRVAHGVPTLwIQLMVHYLKNPPERMSLQEIyVGGSAVPPILIKAWEE 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 318 R-GFSITHAYGMTESYGPSTLCLWQPGVDElplEARAQFMSRQGVAHPMLEEATVLDtdtGRPVPADGRTLGELVVRGNT 396
Cdd:PRK05620 321 RyGVDVVHVWGMTETSPVGTVARPPSGVSG---EARWAYRVSQGRFPASLEYRIVND---GQVMESTDRNEGEIQVRGNW 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 397 VMKGYLHNPEATRAALA-----------------DGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQ 459
Cdd:PRK05620 395 VTASYYHSPTEEGGGAAstfrgedvedandrftaDGWLRTGDVGSVTRDGFLTIHDRARDVIRSGGEWIYSAQLENYIMA 474
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 460 HPEVVEAAVVARPDSRWGETPHAFVALRADALP-RTTGDDLVRWCRERLAHFKAPRHVTLT-ELPKTATGKIQKFVLREW 537
Cdd:PRK05620 475 APEVVECAVIGYPDDKWGERPLAVTVLAPGIEPtRETAERLRDQLRDRLPNWMLPEYWTFVdEIDKTSVGKFDKKDLRQH 554
|
....*
gi 489250734 538 ARQEE 542
Cdd:PRK05620 555 LADGD 559
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
30-535 |
3.26e-64 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 218.01 E-value: 3.26e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 30 AQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSI 109
Cdd:cd05959 14 NEGRGDKTAFIDDAGSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNTLLTPDDY 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 110 AFILRHCAARVLICDREFGAVAQQALAmlDAPPLLVGIDDDQAERADLAHdLDYEAFLAQGDPGRPLSAPQNE----WQs 185
Cdd:cd05959 94 AYYLEDSRARVVVVSGELAPVLAAALT--KSEHTLVVLIVSGGAGPEAGA-LLLAELVAAEAEQLKPAATHADdpafWL- 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 186 iainYTSGTTGDPKGVV-LHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLS-GGTHVCL-RKVQPDA 262
Cdd:cd05959 170 ----YSSGSTGRPKGVVhLHADIYWTAELYARNVLGIREDDVCFSAAKLFFAYGLGNSLTFPLSvGATTVLMpERPTPAA 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 263 IHAAIAEHAVTHLSAAPVVmsmlihaeHASVPAVPVSVITGGAAPPSAVIAAmEArgfsithaygMTESYGPStlclWQP 342
Cdd:cd05959 246 VFKRIRRYRPTVFFGVPTL--------YAAMLAAPNLPSRDLSSLRLCVSAG-EA----------LPAEVGER----WKA 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 343 --GVDEL----PLEARAQFMS-RQGVAHPmleeatvldTDTGRPVP--------------ADGRTlGELVVRGNTVMKGY 401
Cdd:cd05959 303 rfGLDILdgigSTEMLHIFLSnRPGRVRY---------GTTGKPVPgyevelrdedggdvADGEP-GELYVRGPSSATMY 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 402 LHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPH 481
Cdd:cd05959 373 WNNRDKTRDTFQGEWTRTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDGLTKPK 452
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 482 AFVALRadalPRTTG-----DDLVRWCRERLAHFKAPRHVT-LTELPKTATGKIQKFVLR 535
Cdd:cd05959 453 AFVVLR----PGYEDsealeEELKEFVKDRLAPYKYPRWIVfVDELPKTATGKIQRFKLR 508
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
46-535 |
8.52e-64 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 214.51 E-value: 8.52e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 46 YSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDR 125
Cdd:cd05972 1 WSFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVTDA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 126 EfgavaqqalamldappllvgidddqaeradlahdldyeaflaqgDPgrplsapqnewqsIAINYTSGTTGDPKGVVLHH 205
Cdd:cd05972 81 E--------------------------------------------DP-------------ALIYFTSGTTGLPKGVLHTH 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 206 RGAYLNACAGALIFQLGPRSIYlWTL--PMFHCNGWSHTWAVTLSGGTHVC--LRKVQPDAIHAAIAEHAVTHLSAAPVV 281
Cdd:cd05972 104 SYPLGHIPTAAYWLGLRPDDIH-WNIadPGWAKGAWSSFFGPWLLGATVFVyeGPRFDAERILELLERYGVTSFCGPPTA 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 282 MSMLIHAEHASVPAVPVSVITGGAAP--PSAVIAAMEARGFSITHAYGMTESYGPSTLCLWQ---PGvdelplearaqfm 356
Cdd:cd05972 183 YRMLIKQDLSSYKFSHLRLVVSAGEPlnPEVIEWWRAATGLPIRDGYGQTETGLTVGNFPDMpvkPG------------- 249
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 357 sRQGVAHPmLEEATVLDTDtGRPVPADgrTLGELVVRGNTV--MKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIK 434
Cdd:cd05972 250 -SMGRPTP-GYDVAIIDDD-GRELPPG--EEGDIAIKLPPPglFLGYVGDPEKTEASIRGDYYLTGDRAYRDEDGYFWFV 324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 435 DRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALP-RTTGDDLVRWCRERLAHFKAP 513
Cdd:cd05972 325 GRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPsEELAEELQGHVKKVLAPYKYP 404
|
490 500
....*....|....*....|...
gi 489250734 514 RHVTLT-ELPKTATGKIQKFVLR 535
Cdd:cd05972 405 REIEFVeELPKTISGKIRRVELR 427
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
29-528 |
1.64e-63 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 216.67 E-value: 1.64e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 29 AAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARS 108
Cdd:PRK07798 12 VADAVPDRVALVCGDRRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRYVEDE 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 109 IAFILRHCAARVLICDREFGAVAQQALAMLDAPPLLVGIDDDQAErADLAHDLDYEAFLAQGDPGRPLSAPQNEwqSIAI 188
Cdd:PRK07798 92 LRYLLDDSDAVALVYEREFAPRVAEVLPRLPKLRTLVVVEDGSGN-DLLPGAVDYEDALAAGSPERDFGERSPD--DLYL 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 189 NYTSGTTGDPKGVVLHHRGAYLnACAGALIFQLGPRSIYLWTL----------------PMFHCNGWSHTWAVTLSGGTH 252
Cdd:PRK07798 169 LYTGGTTGMPKGVMWRQEDIFR-VLLGGRDFATGEPIEDEEELakraaagpgmrrfpapPLMHGAGQWAAFAALFSGQTV 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 253 VCLRKVqpdaihaaiaehavtHLSAAPVVmsMLIHAEHasvpaVPVSVITGGA-APPsaVIAAMEARG-------FSITh 324
Cdd:PRK07798 248 VLLPDV---------------RFDADEVW--RTIEREK-----VNVITIVGDAmARP--LLDALEARGpydlsslFAIA- 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 325 aygmtesygpSTLCLWQPGVDELPLEARAQF-----------------MSRQGVAHP------MLEEATVLDTDtGRPVP 381
Cdd:PRK07798 303 ----------SGGALFSPSVKEALLELLPNVvltdsigssetgfggsgTVAKGAVHTggprftIGPRTVVLDED-GNPVE 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 382 ADGRTLGELVVRGNtVMKGYLHNPEATRAAL--ADG--WLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVL 457
Cdd:PRK07798 372 PGSGEIGWIARRGH-IPLGYYKDPEKTAETFptIDGvrYAIPGDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEAL 450
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 489250734 458 YQHPEVVEAAVVARPDSRWGETPHAFVALRADALPrtTGDDLVRWCRERLAHFKAPRHVTLT-ELPKTATGK 528
Cdd:PRK07798 451 KAHPDVADALVVGVPDERWGQEVVAVVQLREGARP--DLAELRAHCRSSLAGYKVPRAIWFVdEVQRSPAGK 520
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
25-548 |
2.22e-63 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 217.21 E-value: 2.22e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 25 FLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRL 104
Cdd:PRK06710 29 YVEQMASRYPEKKALHFLGKDITFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTNPLY 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 105 EARSIAFILRHCAARVLIC-DREFGAVAQQALAMLDAPPLLVGIDD------------DQAERADLAHDLD-------YE 164
Cdd:PRK06710 109 TERELEYQLHDSGAKVILClDLVFPRVTNVQSATKIEHVIVTRIADflpfpknllypfVQKKQSNLVVKVSesetihlWN 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 165 AFLAQGDPG-RPLSAPQNEWQsiAINYTSGTTGDPKGVVLHHRGAYLNACAGA--LIFQLGPRSIYLWTLPMFHCNGWSH 241
Cdd:PRK06710 189 SVEKEVNTGvEVPCDPENDLA--LLQYTGGTTGFPKGVMLTHKNLVSNTLMGVqwLYNCKEGEEVVLGVLPFFHVYGMTA 266
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 242 TWAVT-LSGGTHVCLRKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIHA---EHASVPAVPvSVITGGAAPPSAVIAAMEA 317
Cdd:PRK06710 267 VMNLSiMQGYKMVLIPKFDMKMVFEAIKKHKVTLFPGAPTIYIALLNSpllKEYDISSIR-ACISGSAPLPVEVQEKFET 345
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 318 -RGFSITHAYGMTESyGPSTLC--LWQPGVDelplearaqfmSRQGVAHPMlEEATVLDTDTGRPVPADgrTLGELVVRG 394
Cdd:PRK06710 346 vTGGKLVEGYGLTES-SPVTHSnfLWEKRVP-----------GSIGVPWPD-TEAMIMSLETGEALPPG--EIGEIVVKG 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 395 NTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDS 474
Cdd:PRK06710 411 PQIMKGYWNKPEETAAVLQDGWLHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYEHEKVQEVVTIGVPDP 490
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489250734 475 RWGETPHAFVALRADALprTTGDDLVRWCRERLAHFKAPRHVTL-TELPKTATGKIQKFVLREWARQEEEAGDAG 548
Cdd:PRK06710 491 YRGETVKAFVVLKEGTE--CSEEELNQFARKYLAAYKVPKVYEFrDELPKTTVGKILRRVLIEEEKRKNEDEQTG 563
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
30-535 |
6.25e-63 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 214.93 E-value: 6.25e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 30 AQVYPQRDAVVY-----GARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRL 104
Cdd:PRK08008 17 ADVYGHKTALIFessggVVRRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARL 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 105 EARSIAFILRHCAARVLICDREFGAVAQQALAMLDAPPLLVGIDDDQAERADLAHDLDYEAFL--AQGDPGRPLSAPQne 182
Cdd:PRK08008 97 LREESAWILQNSQASLLVTSAQFYPMYRQIQQEDATPLRHICLTRVALPADDGVSSFTQLKAQqpATLCYAPPLSTDD-- 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 183 wqSIAINYTSGTTGDPKGVVLHHR----GAYLNACAGALifqlgpRS--IYLWTLPMFH----CNGwshTWAVTLSGGTH 252
Cdd:PRK08008 175 --TAEILFTSGTTSRPKGVVITHYnlrfAGYYSAWQCAL------RDddVYLTVMPAFHidcqCTA---AMAAFSAGATF 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 253 VCLRKVQPDA--IHAAIAEHAVTHlsaapvvmsmlihaehasvpAVPVSVITGGAAPPSA---------VI--------- 312
Cdd:PRK08008 244 VLLEKYSARAfwGQVCKYRATITE--------------------CIPMMIRTLMVQPPSAndrqhclreVMfylnlsdqe 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 313 -AAMEAR-GFSITHAYGMTESYGpstlclwqpGV-DELPLEARA-QFMSRQGVAHpmleEATVLDtDTGRPVPADgrTLG 388
Cdd:PRK08008 304 kDAFEERfGVRLLTSYGMTETIV---------GIiGDRPGDKRRwPSIGRPGFCY----EAEIRD-DHNRPLPAG--EIG 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 389 ELVVRG---NTVMKGYLHNPEATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVV 464
Cdd:PRK08008 368 EICIKGvpgKTIFKEYYLDPKATAKVLeADGWLHTGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQ 447
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 489250734 465 EAAVVARPDSRWGETPHAFVALRADAlpRTTGDDLVRWCRERLAHFKAPRHVTL-TELPKTATGKIQKFVLR 535
Cdd:PRK08008 448 DIVVVGIKDSIRDEAIKAFVVLNEGE--TLSEEEFFAFCEQNMAKFKVPSYLEIrKDLPRNCSGKIIKKNLK 517
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
26-529 |
1.58e-62 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 213.14 E-value: 1.58e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 26 LKRAAQVYPQRDAVVYGAR--RYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIR 103
Cdd:cd05923 7 LRRAASRAPDACAIADPARglRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALINPR 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 104 LEARSIA-FILRHCAARVLICDrefGAVAQQALAMLDAPPLLVGIDDDQAERadlahdldyEAFlaqgdpGRPLSAPQNE 182
Cdd:cd05923 87 LKAAELAeLIERGEMTAAVIAV---DAQVMDAIFQSGVRVLALSDLVGLGEP---------ESA------GPLIEDPPRE 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 183 WQSIA-INYTSGTTGDPKGVVLHHRGAYLNACAGALIFQL--GPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCL-RKV 258
Cdd:cd05923 149 PEQPAfVFYTSGTTGLPKGAVIPQRAAESRVLFMSTQAGLrhGRHNVVLGLMPLYHVIGFFAVLVAALALDGTYVVvEEF 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 259 QPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPV--SVITGGAAPPSAVIAAM-EARGFSITHAYGMTESYGPS 335
Cdd:cd05923 229 DPADALKLIEQERVTSLFATPTHLDALAAAAEFAGLKLSSlrHVTFAGATMPDAVLERVnQHLPGEKVNIYGTTEAMNSL 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 336 TLCLWQPGVdelplEARAQFMSRQGVAhPMLEEATVLdtdtgrpvPADGRTlGELVVR--GNTVMKGYLHNPEATRAALA 413
Cdd:cd05923 309 YMRDARTGT-----EMRPGFFSEVRIV-RIGGSPDEA--------LANGEE-GELIVAaaADAAFTGYLNQPEATAKKLQ 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 414 DGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRadaLPR 493
Cdd:cd05923 374 DGWYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTACVVPR---EGT 450
|
490 500 510
....*....|....*....|....*....|....*...
gi 489250734 494 TTGDDLVRWCRER-LAHFKAPR-HVTLTELPKTATGKI 529
Cdd:cd05923 451 LSADELDQFCRASeLADFKRPRrYFFLDELPKNAMNKV 488
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
188-531 |
2.32e-61 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 205.43 E-value: 2.32e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 188 INYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTL-SGGTHVCLRKVQPDAIHAA 266
Cdd:cd17638 5 IMFTSGTTGRSKGVMCAHRQTLRAAAAWADCADLTEDDRYLIINPFFHTFGYKAGIVACLlTGATVVPVAVFDVDAILEA 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 267 IAEHAVTHLSAAPVVMSMLIHaeHASVPAVPVS----VITGGAAPPSAVIAAM-EARGF-SITHAYGMTESyGPSTLClw 340
Cdd:cd17638 85 IERERITVLPGPPTLFQSLLD--HPGRKKFDLSslraAVTGAATVPVELVRRMrSELGFeTVLTAYGLTEA-GVATMC-- 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 341 QPGvdelplearaqfmsrqgvahpmlEEATVLDTDTGRPVPADGRTL---GELVVRGNTVMKGYLHNPEATRAAL-ADGW 416
Cdd:cd17638 160 RPG-----------------------DDAETVATTCGRACPGFEVRIaddGEVLVRGYNVMQGYLDDPEATAEAIdADGW 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 417 LHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALprTTG 496
Cdd:cd17638 217 LHTGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVVARPGVT--LTE 294
|
330 340 350
....*....|....*....|....*....|....*.
gi 489250734 497 DDLVRWCRERLAHFKAPRHVT-LTELPKTATGKIQK 531
Cdd:cd17638 295 EDVIAWCRERLANYKVPRFVRfLDELPRNASGKVMK 330
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
30-539 |
1.76e-60 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 208.01 E-value: 1.76e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 30 AQVYPQRDAVVYGA--RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEAR 107
Cdd:PRK13391 7 AQTTPDKPAVIMAStgEVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPA 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 108 SIAFILRHCAARVLICDREFGAVAQQALAMLDA--PPLLVGIDDDQaERADlahdlDYEAFLAqGDPGRPLSapqNEWQS 185
Cdd:PRK13391 87 EAAYIVDDSGARALITSAAKLDVARALLKQCPGvrHRLVLDGDGEL-EGFV-----GYAEAVA-GLPATPIA---DESLG 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 186 IAINYTSGTTGDPKGVV--LHHRG-----AYLNACAGAliFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKV 258
Cdd:PRK13391 157 TDMLYSSGTTGRPKGIKrpLPEQPpdtplPLTAFLQRL--WGFRSDMVYLSPAPLYHSAPQRAVMLVIRLGGTVIVMEHF 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 259 QPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVS---VITGGAAP-PSAVIAAMEARGFSITHA-YGMTESYG 333
Cdd:PRK13391 235 DAEQYLALIEEYGVTHTQLVPTMFSRMLKLPEEVRDKYDLSsleVAIHAAAPcPPQVKEQMIDWWGPIIHEyYAATEGLG 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 334 pSTLClwqpgvdelpleARAQFMSRQG-VAHPMLEEATVLDtDTGRPVPADgrTLGELVVRGNTVMKgYLHNPEATRAAL 412
Cdd:PRK13391 315 -FTAC------------DSEEWLAHPGtVGRAMFGDLHILD-DDGAELPPG--EPGTIWFEGGRPFE-YLNDPAKTAEAR 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 413 -ADG-WLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADA 490
Cdd:PRK13391 378 hPDGtWSTVGDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVQPVDGV 457
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 489250734 491 LPRTT-GDDLVRWCRERLAHFKAPRHVT-LTELPKTATGKIQKFVLRE--WAR 539
Cdd:PRK13391 458 DPGPAlAAELIAFCRQRLSRQKCPRSIDfEDELPRLPTGKLYKRLLRDryWGN 510
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
46-534 |
8.61e-60 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 204.75 E-value: 8.61e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 46 YSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDr 125
Cdd:cd05907 6 ITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKALFVE- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 126 efgavaqqalamldappllvgidddqaERADLAhdldyeaflaqgdpgrplsapqnewqsiAINYTSGTTGDPKGVVLHH 205
Cdd:cd05907 85 ---------------------------DPDDLA----------------------------TIIYTSGTTGRPKGVMLSH 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 206 RGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGW-SHTWAVTLSGGTHVC----------LRKVQPdaihaaiaehavTH 274
Cdd:cd05907 110 RNILSNALALAERLPATEGDRHLSFLPLAHVFERrAGLYVPLLAGARIYFassaetllddLSEVRP------------TV 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 275 LSAAPVVMSMLIHAehASVPAVP---------------VSVITGGAAPPSAVIAAMEARGFSITHAYGMTESYGPSTLCL 339
Cdd:cd05907 178 FLAVPRVWEKVYAA--IKVKAVPglkrklfdlavggrlRFAASGGAPLPAELLHFFRALGIPVYEGYGLTETSAVVTLNP 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 340 WQpgvdelpleaRAQFMSrqgVAHPMLEEATVLDTDtgrpvpadgrtlGELVVRGNTVMKGYLHNPEATRAAL-ADGWLH 418
Cdd:cd05907 256 PG----------DNRIGT---VGKPLPGVEVRIADD------------GEILVRGPNVMLGYYKNPEATAEALdADGWLH 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 419 TGDLAVLHPDGYVEIKDRAKDIII-SGGENISSLEIEEVLYQHPEVVEAAVVAR----------PD----SRW----GET 479
Cdd:cd05907 311 TGDLGEIDEDGFLHITGRKKDLIItSGGKNISPEPIENALKASPLISQAVVIGDgrpflvalivPDpealEAWaeehGIA 390
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 489250734 480 PHAFVALRADALPRTTGDDLVRWCRERLAHFKAPRHVTLTELP-------KTATGKIQKFVL 534
Cdd:cd05907 391 YTDVAELAANPAVRAEIEAAVEAANARLSRYEQIKKFLLLPEPftiengeLTPTLKLKRPVI 452
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
29-536 |
9.97e-60 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 207.09 E-value: 9.97e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 29 AAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARS 108
Cdd:PRK07788 58 AARRAPDRAALIDERGTLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILLNTGFSGPQ 137
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 109 IAFILRHCAARVLICDREFGAVAQQALAmlDAPPLLVGIDDDQAERADLAHDLDYEAFLAQGDPGRPLSAPQNEwqSIAI 188
Cdd:PRK07788 138 LAEVAAREGVKALVYDDEFTDLLSALPP--DLGRLRAWGGNPDDDEPSGSTDETLDDLIAGSSTAPLPKPPKPG--GIVI 213
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 189 nYTSGTTGDPKGVVLHHRGAYlnACAGALIFQ--LGPRSIYLWTLPMFHCNGWSHtWAVTLSGGTHVCL-RKVQPDAIHA 265
Cdd:PRK07788 214 -LTSGTTGTPKGAPRPEPSPL--APLAGLLSRvpFRAGETTLLPAPMFHATGWAH-LTLAMALGSTVVLrRRFDPEATLE 289
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 266 AIAEHAVTHLSAAPVVMS-MLIHAEHAS----VPAVPVSVITGGAAPPSAVIAAMEARGFSITHAYGMTESygpSTLCLW 340
Cdd:PRK07788 290 DIAKHKATALVVVPVMLSrILDLGPEVLakydTSSLKIIFVSGSALSPELATRALEAFGPVLYNLYGSTEV---AFATIA 366
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 341 QPgvDELpleARAQfmsrqGVAHPMLEEATV--LDtDTGRPVPADgrTLGELVVRGNTVMKGYLH--NPEatraaLADGW 416
Cdd:PRK07788 367 TP--EDL---AEAP-----GTVGRPPKGVTVkiLD-ENGNEVPRG--VVGRIFVGNGFPFEGYTDgrDKQ-----IIDGL 428
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 417 LHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAlpRTTG 496
Cdd:PRK07788 429 LSSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQRLRAFVVKAPGA--ALDE 506
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 489250734 497 DDLVRWCRERLAHFKAPRHVT-LTELPKTATGKIQKFVLRE 536
Cdd:PRK07788 507 DAIKDYVRDNLARYKVPRDVVfLDELPRNPTGKVLKRELRE 547
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
24-531 |
6.91e-59 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 202.94 E-value: 6.91e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 24 SFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIR 103
Cdd:cd05920 19 DLLARSAARHPDRIAVVDGDRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGAVPVLALPS 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 104 LEARSIAFILRHCAARVLICDREFGAVAQQALAMldappllvgidddqaeraDLAHDLDYEAFLAqgdpgrpLSapqnew 183
Cdd:cd05920 99 HRRSELSAFCAHAEAVAYIVPDRHAGFDHRALAR------------------ELAESIPEVALFL-------LS------ 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 184 qsiainytSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFH-----CNGwshTWAVTLSGGTHVCLRKV 258
Cdd:cd05920 148 --------GGTTGTPKLIPRTHNDYAYNVRASAEVCGLDQDTVYLAVLPAAHnfplaCPG---VLGTLLAGGRVVLAPDP 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 259 QPDAIHAAIAEHAVTHLSAAP-VVMSMLIHAEHASVPAVPVSVITGGAAPPSAVIAAM--EARGFSITHAYGMTESYgps 335
Cdd:cd05920 217 SPDAAFPLIEREGVTVTALVPaLVSLWLDAAASRRADLSSLRLLQVGGARLSPALARRvpPVLGCTLQQVFGMAEGL--- 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 336 tLCLWQPGvdelplEARAQFMSRQGvaHPM--LEEATVLDTDtGRPVPaDGRTlGELVVRGNTVMKGYLHNPEA-TRAAL 412
Cdd:cd05920 294 -LNYTRLD------DPDEVIIHTQG--RPMspDDEIRVVDEE-GNPVP-PGEE-GELLTRGPYTIRGYYRAPEHnARAFT 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 413 ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRAdalP 492
Cdd:cd05920 362 PDGFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAMPDELLGERSCAFVVLRD---P 438
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 489250734 493 RTTGDDLVRWCRER-LAHFKAP-RHVTLTELPKTATGKIQK 531
Cdd:cd05920 439 PPSAAQLRRFLRERgLAAYKLPdRIEFVDSLPLTAVGKIDK 479
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
42-536 |
1.20e-56 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 197.79 E-value: 1.20e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 42 GARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVL 121
Cdd:PRK07514 25 DGLRYTYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGAVFLPLNTAYTLAELDYFIGDAEPALV 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 122 ICD-REFGAVAQQALAmLDAPPLLVGIDDDQAERADLAHDLDyEAFLAqgdpgrplsAPQNEWQSIAINYTSGTTGDPKG 200
Cdd:PRK07514 105 VCDpANFAWLSKIAAA-AGAPHVETLDADGTGSLLEAAAAAP-DDFET---------VPRGADDLAAILYTSGTTGRSKG 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 201 VVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGW---SHTwaVTLSGGTHVCLRKVQPDAIhaaiaehaVTHLSA 277
Cdd:PRK07514 174 AMLSHGNLLSNALTLVDYWRFTPDDVLIHALPIFHTHGLfvaTNV--ALLAGASMIFLPKFDPDAV--------LALMPR 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 278 APVVMS-------MLIHAEHASVPAVPVSVITGGAAPPSA-VIAAMEAR-GFSITHAYGMTES-------YG----PSTL 337
Cdd:PRK07514 244 ATVMMGvptfytrLLQEPRLTREAAAHMRLFISGSAPLLAeTHREFQERtGHAILERYGMTETnmntsnpYDgerrAGTV 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 338 CLWQPGVdelplearaqfmsrqgvahpmleEATVLDTDTGRPVPADGrtLGELVVRGNTVMKGYLHNPEATRAAL-ADGW 416
Cdd:PRK07514 324 GFPLPGV-----------------------SLRVTDPETGAELPPGE--IGMIEVKGPNVFKGYWRMPEKTAEEFrADGF 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 417 LHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPrtTG 496
Cdd:PRK07514 379 FITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVVESAVIGVPHPDFGEGVTAVVVPKPGAAL--DE 456
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 489250734 497 DDLVRWCRERLAHFKAPRHVT-LTELPKTATGKIQKFVLRE 536
Cdd:PRK07514 457 AAILAALKGRLARFKQPKRVFfVDELPRNTMGKVQKNLLRE 497
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
36-535 |
3.96e-56 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 194.60 E-value: 3.96e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 36 RDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRH 115
Cdd:cd05919 1 KTAFYAADRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARD 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 116 CAARVLIcdrefgavaqqalamldappllvgIDDDQAeradlahdldyeAFLaqgdpgrplsapqnewqsiaiNYTSGTT 195
Cdd:cd05919 81 CEARLVV------------------------TSADDI------------AYL---------------------LYSSGTT 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 196 GDPKGVVLHHRGAYLNACAGAL-IFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCL--RKVQPDAIHAAIAEHAV 272
Cdd:cd05919 104 GPPKGVMHAHRDPLLFADAMAReALGLTPGDRVFSSAKMFFGYGLGNSLWFPLAVGASAVLnpGWPTAERVLATLARFRP 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 273 THLSAAPVVMSMLI---HAEHASVPAVPVSVITGGAAPPSAVIAAMEARGFSITHAYGMTESyGPSTLC----LWQPGVD 345
Cdd:cd05919 184 TVLYGVPTFYANLLdscAGSPDALRSLRLCVSAGEALPRGLGERWMEHFGGPILDGIGATEV-GHIFLSnrpgAWRLGST 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 346 ELPLEARaqfmsrqgvahpmleEATVLDtDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAALADGWLHTGDLAVL 425
Cdd:cd05919 263 GRPVPGY---------------EIRLVD-EEGHTIPPG--EEGDLLVRGPSAAVGYWNNPEKSRATFNGGWYRTGDKFCR 324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 426 HPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPR-TTGDDLVRWCR 504
Cdd:cd05919 325 DADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAAPQeSLARDIHRHLL 404
|
490 500 510
....*....|....*....|....*....|..
gi 489250734 505 ERLAHFKAPRHVT-LTELPKTATGKIQKFVLR 535
Cdd:cd05919 405 ERLSAHKVPRRIAfVDELPRTATGKLQRFKLR 436
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
186-529 |
5.21e-55 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 188.63 E-value: 5.21e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 186 IAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPDAIHA 265
Cdd:cd17637 3 FVIIHTAAVAGRPRGAVLSHGNLIAANLQLIHAMGLTEADVYLNMLPLFHIAGLNLALATFHAGGANVVMEKFDPAEALE 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 266 AIAEHAVTHLSAAPVVMSMLIHAEHASVPAVP-VSVITGGAAPpsAVIAAMEArgfsITHA-----YGMTESYGPSTLCL 339
Cdd:cd17637 83 LIEEEKVTLMGSFPPILSNLLDAAEKSGVDLSsLRHVLGLDAP--ETIQRFEE----TTGAtfwslYGQTETSGLVTLSP 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 340 wqpgVDELPLEAraqfmsrqGVAHPMLEEATVLDTDtgRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAALADGWLHT 419
Cdd:cd17637 157 ----YRERPGSA--------GRPGPLVRVRIVDDND--RPVPAG--ETGEIVVRGPLVFQGYWNLPELTAYTFRNGWHHT 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 420 GDLAVLHPDGYVEIKDR--AKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPrtTGD 497
Cdd:cd17637 221 GDLGRFDEDGYLWYAGRkpEKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGEGIKAVCVLKPGATL--TAD 298
|
330 340 350
....*....|....*....|....*....|...
gi 489250734 498 DLVRWCRERLAHFKAPRHVTLTE-LPKTATGKI 529
Cdd:cd17637 299 ELIEFVGSRIARYKKPRYVVFVEaLPKTADGSI 331
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
34-529 |
4.52e-54 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 189.28 E-value: 4.52e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 34 PQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFIL 113
Cdd:cd05930 1 PDAVAVVDGDQSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAERLAYIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 114 RHCAARVLICDREfgavaqqalamldappllvgidddqaeraDLAHdldyeaflaqgdpgrplsapqnewqsiaINYTSG 193
Cdd:cd05930 81 EDSGAKLVLTDPD-----------------------------DLAY----------------------------VIYTSG 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 194 TTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYL-WTLPMFHCNGWShTWAVTLSGGTHVCLRKVQ---PDAIHAAIAE 269
Cdd:cd05930 104 STGKPKGVMVEHRGLVNLLLWMQEAYPLTPGDRVLqFTSFSFDVSVWE-IFGALLAGATLVVLPEEVrkdPEALADLLAE 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 270 HAVTHLSAAPVVMSMLIHAEHASVPAVPVSVITGGAAPPSAVIAAMEARGFS--ITHAYGMTESYGPSTLClwqpGVDEL 347
Cdd:cd05930 183 EGITVLHLTPSLLRLLLQELELAALPSLRLVLVGGEALPPDLVRRWRELLPGarLVNLYGPTEATVDATYY----RVPPD 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 348 PLEARA----QFMSRQGVAhpmleeatVLDtDTGRPVPaDGRTlGELVVRGNTVMKGYLHNPEATRAAL------ADGWL 417
Cdd:cd05930 259 DEEDGRvpigRPIPNTRVY--------VLD-ENLRPVP-PGVP-GELYIGGAGLARGYLNRPELTAERFvpnpfgPGERM 327
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 418 H-TGDLAVLHPDGYVEIKDRAKDII-ISG-----GEnissleIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADa 490
Cdd:cd05930 328 YrTGDLVRWLPDGNLEFLGRIDDQVkIRGyrielGE------IEAALLAHPGVREAAVVAREDGDGEKRLVAYVVPDEG- 400
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 489250734 491 lPRTTGDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKI 529
Cdd:cd05930 401 -GELDEEELRAHLAERLPDYMVPSAfVVLDALPLTPNGKV 439
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
36-535 |
5.82e-54 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 188.84 E-value: 5.82e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 36 RDAVVYGARRYSYRQLHERSCALASAL-ERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILR 114
Cdd:cd05958 1 RTCLRSPEREWTYRDLLALANRIANVLvGELGIVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILD 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 115 HCAARVLICDREFGAVaqqalamldappllvgidDDQAeradlahdldyeaflaqgdpgrplsapqnewqsiAINYTSGT 194
Cdd:cd05958 81 KARITVALCAHALTAS------------------DDIC----------------------------------ILAFTSGT 108
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 195 TGDPKGVVLHHRgAYLNACAGALIFQLGPR--SIYLWTLPMFHCNGWSHTWAVTLS-GGTHVCLRKVQPDAIHAAIAEHA 271
Cdd:cd05958 109 TGAPKATMHFHR-DPLASADRYAVNVLRLRedDRFVGSPPLAFTFGLGGVLLFPFGvGASGVLLEEATPDLLLSAIARYK 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 272 VTHLSAAPVVMSMLIHAEHASVP---AVPVSVITGGAAPPSAVIAAMEARGFSITHAYGMTESYgpstlclwQPGVDELP 348
Cdd:cd05958 188 PTVLFTAPTAYRAMLAHPDAAGPdlsSLRKCVSAGEALPAALHRAWKEATGIPIIDGIGSTEMF--------HIFISARP 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 349 LEARAqfmSRQGVAHPMLeEATVLDtDTGRPVPADgrTLGELVVRGNTvmkGYLHNPEATRA-ALADGWLHTGDLAVLHP 427
Cdd:cd05958 260 GDARP---GATGKPVPGY-EAKVVD-DEGNPVPDG--TIGRLAVRGPT---GCRYLADKRQRtYVQGGWNITGDTYSRDP 329
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 428 DGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPrttGDDLVR----WC 503
Cdd:cd05958 330 DGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIP---GPVLARelqdHA 406
|
490 500 510
....*....|....*....|....*....|...
gi 489250734 504 RERLAHFKAPRHVT-LTELPKTATGKIQKFVLR 535
Cdd:cd05958 407 KAHIAPYKYPRAIEfVTELPRTATGKLQRFALR 439
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
25-531 |
3.84e-53 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 187.79 E-value: 3.84e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 25 FLKRAAQvYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRL 104
Cdd:cd12117 3 FEEQAAR-TPDAVAVVYGDRSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPEL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 105 EARSIAFILRHCAARVLICDREFGAVAQQalamlDAPPLLVGIDDDQAEradlahdldyeaflaQGDPGRPLSApqnewQ 184
Cdd:cd12117 82 PAERLAFMLADAGAKVLLTDRSLAGRAGG-----LEVAVVIDEALDAGP---------------AGNPAVPVSP-----D 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 185 SIA-INYTSGTTGDPKGVVLHHRG--------AYLNACAGALIFQLGPRSIYLWTLPMfhcngwshtWAVTLSGGTHVCL 255
Cdd:cd12117 137 DLAyVMYTSGSTGRPKGVAVTHRGvvrlvkntNYVTLGPDDRVLQTSPLAFDASTFEI---------WGALLNGARLVLA 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 256 RK---VQPDAIHAAIAEHAVT--HLSAApvvMSMLIHAEHASVPAVPVSVITGG-AAPPSAVIAAMEAR-GFSITHAYGM 328
Cdd:cd12117 208 PKgtlLDPDALGALIAEEGVTvlWLTAA---LFNQLADEDPECFAGLRELLTGGeVVSPPHVRRVLAACpGLRLVNGYGP 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 329 TESYGPSTLCLwqpgVDELPLEARAQFMSRQgVAHpmlEEATVLDTDtGRPVPADgrTLGELVVRGNTVMKGYLHNPEAT 408
Cdd:cd12117 285 TENTTFTTSHV----VTELDEVAGSIPIGRP-IAN---TRVYVLDED-GRPVPPG--VPGELYVGGDGLALGYLNRPALT 353
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 409 RAA-LADGWL------HTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPH 481
Cdd:cd12117 354 AERfVADPFGpgerlyRTGDLARWLPDGRLEFLGRIDDQVKIRGFRIELGEIEAALRAHPGVREAVVVVREDAGGDKRLV 433
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 489250734 482 AFVALRADALPrttgDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQK 531
Cdd:cd12117 434 AYVVAEGALDA----AELRAFLRERLPAYMVPAAfVVLDELPLTANGKVDR 480
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
36-537 |
4.30e-53 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 187.12 E-value: 4.30e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 36 RDAVVYGARRYSYRQLHERSCALAsalERVGvqAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRH 115
Cdd:PRK07787 16 ADAVRIGGRVLSRSDLAGAATAVA---ERVA--GARRVAVLATPTLATVLAVVGALIAGVPVVPVPPDSGVAERRHILAD 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 116 CAARvlicdrefgavaqqalAMLDAPPllvgidDDQAERADLAHDLDyeaflAQGDPGRPLSAPQnewqSIA-INYTSGT 194
Cdd:PRK07787 91 SGAQ----------------AWLGPAP------DDPAGLPHVPVRLH-----ARSWHRYPEPDPD----APAlIVYTSGT 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 195 TGDPKGVVLHHRG--AYLNACAGAliFQLGPRSIYLWTLPMFHCNGwshtwaVTLS-------GGTHVCLRKVQPDAIHA 265
Cdd:PRK07787 140 TGPPKGVVLSRRAiaADLDALAEA--WQWTADDVLVHGLPLFHVHG------LVLGvlgplriGNRFVHTGRPTPEAYAQ 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 266 AIAEHAvTHLSAAPVVMSMLIHAEHASVPAVPVSVITGGAAP-PSAVIAAMEAR-GFSITHAYGMTEsygpsTLclwqpg 343
Cdd:PRK07787 212 ALSEGG-TLYFGVPTVWSRIAADPEAARALRGARLLVSGSAAlPVPVFDRLAALtGHRPVERYGMTE-----TL------ 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 344 vdeLPLEARAQFMSRQG-VAHPMLEEATVLDTDTGRPVPADGRTLGELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGD 421
Cdd:PRK07787 280 ---ITLSTRADGERRPGwVGLPLAGVETRLVDEDGGPVPHDGETVGELQVRGPTLFDGYLNRPDATAAAFtADGWFRTGD 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 422 LAVLHPDGYVEIKDR-AKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPrttgDDLV 500
Cdd:PRK07787 357 VAVVDPDGMHRIVGReSTDLIKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAYVVGADDVAA----DELI 432
|
490 500 510
....*....|....*....|....*....|....*...
gi 489250734 501 RWCRERLAHFKAPRHVT-LTELPKTATGKIQKFVLREW 537
Cdd:PRK07787 433 DFVAQQLSVHKRPREVRfVDALPRNAMGKVLKKQLLSE 470
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
24-531 |
7.31e-53 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 188.17 E-value: 7.31e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 24 SFLKRAAQVYPQRDAVVYGARR--YSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCIN 101
Cdd:PRK05852 20 DLVEVAATRLPEAPALVVTADRiaISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLVVVPLD 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 102 IRLEARSIAFILRHCAARVLICDREfgAVAQQALAMLDAPPLLVGI-DDDQAERADLAHDLDyeaflAQGDPGRPLSAPQ 180
Cdd:PRK05852 100 PALPIAEQRVRSQAAGARVVLIDAD--GPHDRAEPTTRWWPLTVNVgGDSGPSGGTLSVHLD-----AATEPTPATSTPE 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 181 NEWQSIA-INYTSGTTGDPKGVVLHHrgAYLNACAGALI--FQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLR- 256
Cdd:PRK05852 173 GLRPDDAmIMFTGGTTGLPKMVPWTH--ANIASSVRAIItgYRLSPRDATVAVMPLYHGHGLIAALLATLASGGAVLLPa 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 257 --KVQPDAIHAAIAEHAVTHLSAAPVVMSMLIH---AEHASVPAVPVSVITGGAAP--PSAVIAAMEARGFSITHAYGMT 329
Cdd:PRK05852 251 rgRFSAHTFWDDIKAVGATWYTAVPTIHQILLEraaTEPSGRKPAALRFIRSCSAPltAETAQALQTEFAAPVVCAFGMT 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 330 E-SYGPSTLCLWQPGVDELPLEARAQFMSRQGVahpmleEATVLDTDtGRPVPADgrTLGELVVRGNTVMKGYLHNPEAT 408
Cdd:PRK05852 331 EaTHQVTTTQIEGIGQTENPVVSTGLVGRSTGA------QIRIVGSD-GLPLPAG--AVGEVWLRGTTVVRGYLGDPTIT 401
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 409 RAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRA 488
Cdd:PRK05852 402 AANFTDGWLRTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGEAVAAVIVPRE 481
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 489250734 489 DALPrtTGDDLVRWCRERLAHFKAP-RHVTLTELPKTATGKIQK 531
Cdd:PRK05852 482 SAPP--TAEELVQFCRERLAAFEIPaSFQEASGLPHTAKGSLDR 523
|
|
| ligase_PEP_1 |
TIGR03098 |
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ... |
20-534 |
9.03e-53 |
|
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.
Pssm-ID: 211788 [Multi-domain] Cd Length: 517 Bit Score: 187.68 E-value: 9.03e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 20 LSPLsfLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVC 99
Cdd:TIGR03098 2 LHHL--LEDAAARLPDATALVHHDRTLTYAALSERVLALASGLRGLGLARGERVAIYLDKRLETVTAMFGAALAGGVFVP 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 100 INIRLEARSIAFILRHCAARVLICDREFGAVAQQALAMLDAPPLLVGIDDDQAERADLAHD--LDYEAFLAQGDPGRPLS 177
Cdd:TIGR03098 80 INPLLKAEQVAHILADCNVRLLVTSSERLDLLHPALPGCHDLRTLIIVGDPAHASEGHPGEepASWPKLLALGDADPPHP 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 178 APQNEWQsiAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRK 257
Cdd:TIGR03098 160 VIDSDMA--AILYTSGSTGRPKGVVLSHRNLVAGAQSVATYLENRPDDRLLAVLPLSFDYGFNQLTTAFYVGATVVLHDY 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 258 VQPDAIHAAIAEHAVTHLSAAPVVMSMLihAEHASVPAVPVSVI----TGGAAPPSAVIA----AMEARGFSIthaYGMT 329
Cdd:TIGR03098 238 LLPRDVLKALEKHGITGLAAVPPLWAQL--AQLDWPESAAPSLRyltnSGGAMPRATLSRlrsfLPNARLFLM---YGLT 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 330 ESYGPSTLclwQPG-VDELPlearaqfmSRQGVAHPMLeEATVLDTDTGRPVPADgrtLGELVVRGNTVMKGYLHNPEAT 408
Cdd:TIGR03098 313 EAFRSTYL---PPEeVDRRP--------DSIGKAIPNA-EVLVLREDGSECAPGE---EGELVHRGALVAMGYWNDPEKT 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 409 RAALA-----DGWLHTGDLAVLHPD-------GYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRW 476
Cdd:TIGR03098 378 AERFRplppfPGELHLPELAVWSGDtvrrdeeGFLYFVGRRDEMIKTSGYRVSPTEVEEVAYATGLVAEAVAFGVPDPTL 457
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 489250734 477 GETPHAFVALRADAlpRTTGDDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKIQKFVL 534
Cdd:TIGR03098 458 GQAIVLVVTPPGGE--ELDRAALLAECRARLPNYMVPALIHVRQaLPRNANGKIDRKAL 514
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
38-538 |
1.42e-52 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 186.83 E-value: 1.42e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 38 AVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCA 117
Cdd:PRK12406 4 TIISGDRRRSFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSG 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 118 ARVLICDRE-FGAVAQ---QALAMLDA--PPLLV---GIDDdqAERADLAHDLDYEAFLAQGDP--GRPLSAPQNewqsi 186
Cdd:PRK12406 84 ARVLIAHADlLHGLASalpAGVTVLSVptPPEIAaayRISP--ALLTPPAGAIDWEGWLAQQEPydGPPVPQPQS----- 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 187 aINYTSGTTGDPKGVVLHHRGAYLNACAG---ALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPDAI 263
Cdd:PRK12406 157 -MIYTSGTTGHPKGVRRAAPTPEQAAAAEqmrALIYGLKPGIRALLTGPLYHSAPNAYGLRAGRLGGVLVLQPRFDPEEL 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 264 HAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVS----VITGGAAPPSAVIAAM-EARGFSITHAYGMTESyGPSTLC 338
Cdd:PRK12406 236 LQLIERHRITHMHMVPTMFIRLLKLPEEVRAKYDVSslrhVIHAAAPCPADVKRAMiEWWGPVIYEYYGSTES-GAVTFA 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 339 -----LWQPG-VDELPLEARAQFMSrqgvahpmleeatvldtDTGRPVPADgrTLGELVVR--GNTVMKgYLHNPEATRA 410
Cdd:PRK12406 315 tsedaLSHPGtVGKAAPGAELRFVD-----------------EDGRPLPQG--EIGEIYSRiaGNPDFT-YHNKPEKRAE 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 411 ALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADA 490
Cdd:PRK12406 375 IDRGGFITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALMAVVEPQPGA 454
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 489250734 491 LPRTtgDDLVRWCRERLAHFKAPRHVTL-TELPKTATGKIQKFVLRE--WA 538
Cdd:PRK12406 455 TLDE--ADIRAQLKARLAGYKVPKHIEImAELPREDSGKIFKRRLRDpyWA 503
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
20-536 |
4.13e-52 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 186.12 E-value: 4.13e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 20 LSPLSFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVC 99
Cdd:PRK13382 43 MGPTSGFAIAAQRCPDRPGLIDELGTLTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGADILL 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 100 INIRLEARSIAFILRHCAARVLICDREFGAVAQQAlamLDAPPLLVGIDDDQAERADLAHDLDYEAFLAQgdpgRPLSAP 179
Cdd:PRK13382 123 LNTSFAGPALAEVVTREGVDTVIYDEEFSATVDRA---LADCPQATRIVAWTDEDHDLTVEVLIAAHAGQ----RPEPTG 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 180 QnewQSIAINYTSGTTGDPKGVVLHHRGAYLNACAgalIFQLGP----RSIYLwTLPMFHCNGWSHTWAVTLSGGTHVCL 255
Cdd:PRK13382 196 R---KGRVILLTSGTTGTPKGARRSGPGGIGTLKA---ILDRTPwraeEPTVI-VAPMFHAWGFSQLVLAASLACTIVTR 268
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 256 RKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIHA-----EHASVPAVPVSVITGGAAPPSAVIAAMEARGFSITHAYGMTE 330
Cdd:PRK13382 269 RRFDPEATLDLIDRHRATGLAVVPVMFDRIMDLpaevrNRYSGRSLRFAAASGSRMRPDVVIAFMDQFGDVIYNNYNATE 348
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 331 SYGPSTlclwqpgvdELPLEARAQfMSRQGVAhPMLEEATVLDTDtGRPVPaDGRTlGELVVRGNTVMKGYlhNPEATRa 410
Cdd:PRK13382 349 AGMIAT---------ATPADLRAA-PDTAGRP-AEGTEIRILDQD-FREVP-TGEV-GTIFVRNDTQFDGY--TSGSTK- 411
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 411 ALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADA 490
Cdd:PRK13382 412 DFHDGFMASGDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLAAFVVLKPGA 491
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 489250734 491 lpRTTGDDLVRWCRERLAHFKAPRHVT-LTELPKTATGKIQKFVLRE 536
Cdd:PRK13382 492 --SATPETLKQHVRDNLANYKVPRDIVvLDELPRGATGKILRRELQA 536
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
191-538 |
1.93e-51 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 178.68 E-value: 1.93e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 191 TSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPdaIHAAIAEH 270
Cdd:cd17630 8 TSGSTGTPKAVVHTAANLLASAAGLHSRLGFGGGDSWLLSLPLYHVGGLAILVRSLLAGAELVLLERNQA--LAEDLAPP 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 271 AVTHLSAAPVVMSMLI--HAEHASVPAVPVsVITGGAAPPSAVIAAMEARGFSITHAYGMTESygPSTLCLWQPGVDElp 348
Cdd:cd17630 86 GVTHVSLVPTQLQRLLdsGQGPAALKSLRA-VLLGGAPIPPELLERAADRGIPLYTTYGMTET--ASQVATKRPDGFG-- 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 349 learaqfmsrqgvahpmleeatvlDTDTGRPVPadGRTL-----GELVVRGNTVMKGYLhNPEATRAALADGWLHTGDLA 423
Cdd:cd17630 161 ------------------------RGGVGVLLP--GRELrivedGEIWVGGASLAMGYL-RGQLVPEFNEDGWFTTKDLG 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 424 VLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPrttgDDLVRWC 503
Cdd:cd17630 214 ELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGRGPADP----AELRAWL 289
|
330 340 350
....*....|....*....|....*....|....*.
gi 489250734 504 RERLAHFKAPRHVT-LTELPKTATGKIQKFVLREWA 538
Cdd:cd17630 290 KDKLARFKLPKRIYpVPELPRTGGGKVDRRALRAWL 325
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
39-534 |
1.23e-50 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 180.33 E-value: 1.23e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 39 VVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAA 118
Cdd:cd05914 1 LYYGGEPLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 119 RVLICDREfgavaqqalamldappllvgidDDQAeradlahdldyeaflaqgdpgrplsapqnewqsiAINYTSGTTGDP 198
Cdd:cd05914 81 KAIFVSDE----------------------DDVA----------------------------------LINYTSGTTGNS 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 199 KGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPDAIHAAIAEHAVTHLSAA 278
Cdd:cd05914 105 KGVMLTYRNIVSNVDGVKEVVLLGKGDKILSILPLHHIYPLTFTLLLPLLNGAHVVFLDKIPSAKIIALAFAQVTPTLGV 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 279 PVVMSMLIHAEHASVP-----------AVPVSV----------------------ITGGAAPPSAVIAAMEARGFSITHA 325
Cdd:cd05914 185 PVPLVIEKIFKMDIIPkltlkkfkfklAKKINNrkirklafkkvheafggnikefVIGGAKINPDVEEFLRTIGFPYTIG 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 326 YGMTESyGPsTLCLWQPGvdelpleaRAQFMSrQGVAHPMLEEAtvldTDTGRPVPADgrtlGELVVRGNTVMKGYLHNP 405
Cdd:cd05914 265 YGMTET-AP-IISYSPPN--------RIRLGS-AGKVIDGVEVR----IDSPDPATGE----GEIIVRGPNVMKGYYKNP 325
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 406 EATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISG-GENISSLEIEEVLYQHPEVVEAAVVARpDSRWGETPHA- 482
Cdd:cd05914 326 EATAEAFdKDGWFHTGDLGKIDAEGYLYIRGRKKEMIVLSsGKNIYPEEIEAKINNMPFVLESLVVVQ-EKKLVALAYId 404
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 489250734 483 FVALRADALPRTTGDDLVRW-----CRERLAHFKAPRHVTL--TELPKTATGKIQKFVL 534
Cdd:cd05914 405 PDFLDVKALKQRNIIDAIKWevrdkVNQKVPNYKKISKVKIvkEEFEKTPKGKIKRFLY 463
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
46-538 |
3.28e-50 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 178.85 E-value: 3.28e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 46 YSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDR 125
Cdd:cd05969 1 YTFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITTE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 126 EFgavaqqalamldappllvgidddqAERADLAhdldyeaflaqgDPgrplsapqnewqsIAINYTSGTTGDPKGVVLHH 205
Cdd:cd05969 81 EL------------------------YERTDPE------------DP-------------TLLHYTSGTTGTPKGVLHVH 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 206 RGAYLNACAGALIFQLGPRSIYLWTL-PMFHCNGWSHTWAVTLSGGTHVCLR-KVQPDAIHAAIAEHAVTHLSAAPVVMS 283
Cdd:cd05969 112 DAMIFYYFTGKYVLDLHPDDIYWCTAdPGWVTGTVYGIWAPWLNGVTNVVYEgRFDAESWYGIIERVKVTVWYTAPTAIR 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 284 MLIHAEHASVPAVPVS---VITGGAAP--PSAVIAAMEARGFSITHAYGMTESyGPSTLCLWqPGvdeLPLEARAQFMSR 358
Cdd:cd05969 192 MLMKEGDELARKYDLSslrFIHSVGEPlnPEAIRWGMEVFGVPIHDTWWQTET-GSIMIANY-PC---MPIKPGSMGKPL 266
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 359 QGVahpmleEATVLDTDtGRPVPADgrTLGELVVRGN--TVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDR 436
Cdd:cd05969 267 PGV------KAAVVDEN-GNELPPG--TKGILALKPGwpSMFRGIWNDEERYKNSFIDGWYLTGDLAYRDEDGYFWFVGR 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 437 AKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPRTT-GDDLVRWCRERLAHFKAPRH 515
Cdd:cd05969 338 ADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDElKEEIINFVRQKLGAHVAPRE 417
|
490 500
....*....|....*....|....
gi 489250734 516 VTLTE-LPKTATGKIQKFVLREWA 538
Cdd:cd05969 418 IEFVDnLPKTRSGKIMRRVLKAKE 441
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
15-536 |
1.57e-49 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 179.02 E-value: 1.57e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 15 ANHVPLSPLSFlKRAAQvYPQRDAVVYGA--RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPG 92
Cdd:PLN02246 20 PNHLPLHDYCF-ERLSE-FSDRPCLIDGAtgRVYTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASR 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 93 AGAVLVCINIRLEARSIAFILRHCAARVLICDrefgAVAQQALAMLDAPP--LLVGIDDDqaeRADLAHDLDyeafLAQG 170
Cdd:PLN02246 98 RGAVTTTANPFYTPAEIAKQAKASGAKLIITQ----SCYVDKLKGLAEDDgvTVVTIDDP---PEGCLHFSE----LTQA 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 171 DPGRPLSAPQNEWQSIAINYTSGTTGDPKGVVLHHRGayLNACAGALI------FQLGPRSIYLWTLPMFHCNGWShtwA 244
Cdd:PLN02246 167 DENELPEVEISPDDVVALPYSSGTTGLPKGVMLTHKG--LVTSVAQQVdgenpnLYFHSDDVILCVLPMFHIYSLN---S 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 245 VTLS----GGTHVCLRKVQPDAIHAAIAEHAVThlsAAPVVMSMLIhaEHASVPAV------PVSVITGGAAP------- 307
Cdd:PLN02246 242 VLLCglrvGAAILIMPKFEIGALLELIQRHKVT---IAPFVPPIVL--AIAKSPVVekydlsSIRMVLSGAAPlgkeled 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 308 ------PSAVIAameargfsitHAYGMTESyGPS-TLCLwqpgvdelpLEARAQFMSRQGVAHPMLE--EATVLDTDTGR 378
Cdd:PLN02246 317 afraklPNAVLG----------QGYGMTEA-GPVlAMCL---------AFAKEPFPVKSGSCGTVVRnaELKIVDPETGA 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 379 PVPadgRTL-GELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEV 456
Cdd:PLN02246 377 SLP---RNQpGEICIRGPQIMKGYLNDPEATANTIdKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAL 453
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 457 LYQHPEVVEAAVVARPDSRWGETPHAFVALRADAlpRTTGDDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKIQKFVLR 535
Cdd:PLN02246 454 LISHPSIADAAVVPMKDEVAGEVPVAFVVRSNGS--EITEDEIKQFVAKQVVFYKRIHKVFFVDsIPKAPSGKILRKDLR 531
|
.
gi 489250734 536 E 536
Cdd:PLN02246 532 A 532
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
20-535 |
4.34e-49 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 178.45 E-value: 4.34e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 20 LSPLSFLKRAAQVypqrdaVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVC 99
Cdd:PLN02860 13 LTRLATLRGNAVV------TISGNRRRTGHEFVDGVLSLAAGLLRLGLRNGDVVAIAALNSDLYLEWLLAVACAGGIVAP 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 100 INIRL---EARSIAFILRhcaARVLICDRefGAVAQQALAMLDAPPLL---VGIDDDQAERADLAHDLDYEAFLAQGDPG 173
Cdd:PLN02860 87 LNYRWsfeEAKSAMLLVR---PVMLVTDE--TCSSWYEELQNDRLPSLmwqVFLESPSSSVFIFLNSFLTTEMLKQRALG 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 174 RPL----SAPQNewqSIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSG 249
Cdd:PLN02860 162 TTEldyaWAPDD---AVLICFTSGTTGRPKGVTISHSALIVQSLAKIAIVGYGEDDVYLHTAPLCHIGGLSSALAMLMVG 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 250 GTHVCLRKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVP-AVPVSVIT----GGAAP----PSAVIAAMEARGF 320
Cdd:PLN02860 239 ACHVLLPKFDAKAALQAIKQHNVTSMITVPAMMADLISLTRKSMTwKVFPSVRKilngGGSLSsrllPDAKKLFPNAKLF 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 321 SithAYGMTESYGPST-LCLWQPGVDELPLEARAQFMSRQGVAHPmLEEATVldtdtGRPVP--------ADGRTLGELV 391
Cdd:PLN02860 319 S---AYGMTEACSSLTfMTLHDPTLESPKQTLQTVNQTKSSSVHQ-PQGVCV-----GKPAPhvelkiglDESSRVGRIL 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 392 VRGNTVMKGYLHNPEATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVA 470
Cdd:PLN02860 390 TRGPHVMLGYWGQNSETASVLsNDGWLDTGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVG 469
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 471 RPDSRWGETPHAFVALR-----ADALPRTTG-------DDLVRWCRER-LAHFKAPRHVTLTE--LPKTATGKIQKFVLR 535
Cdd:PLN02860 470 VPDSRLTEMVVACVRLRdgwiwSDNEKENAKknltlssETLRHHCREKnLSRFKIPKLFVQWRkpFPLTTTGKIRRDEVR 549
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
46-536 |
7.75e-49 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 176.95 E-value: 7.75e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 46 YSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDR 125
Cdd:cd17642 45 YSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNERELDHSLNISKPTIVFCSK 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 126 E-FGAVA--QQALAMLDAPPLLVGIDDDQAERADlahdldyEAFLAQGDPG-------RPLSAPQNEWQSIAINyTSGTT 195
Cdd:cd17642 125 KgLQKVLnvQKKLKIIKTIIILDSKEDYKGYQCL-------YTFITQNLPPgfneydfKPPSFDRDEQVALIMN-SSGST 196
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 196 GDPKGVVLHHRgaylNACAG-------ALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPDAIHAAIA 268
Cdd:cd17642 197 GLPKGVQLTHK----NIVARfshardpIFGNQIIPDTAILTVIPFHHGFGMFTTLGYLICGFRVVLMYKFEEELFLRSLQ 272
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 269 EHAVTHLSAAPVVMSMLihAEHASVPAVPVS---VITGGAAPPSAVIAAMEARGFS---ITHAYGMTESygpSTLCLWQP 342
Cdd:cd17642 273 DYKVQSALLVPTLFAFF--AKSTLVDKYDLSnlhEIASGGAPLSKEVGEAVAKRFKlpgIRQGYGLTET---TSAILITP 347
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 343 GVDELPlearaqfmSRQGVAHPMLEeATVLDTDTGRPVPADGRtlGELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGD 421
Cdd:cd17642 348 EGDDKP--------GAVGKVVPFFY-AKVVDLDTGKTLGPNER--GELCVKGPMIMKGYVNNPEATKALIdKDGWLHSGD 416
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 422 LAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAlpRTTGDDLVR 501
Cdd:cd17642 417 IAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDAGELPAAVVVLEAGK--TMTEKEVMD 494
|
490 500 510
....*....|....*....|....*....|....*..
gi 489250734 502 WCRERLAHFKAPRH--VTLTELPKTATGKIQKFVLRE 536
Cdd:cd17642 495 YVASQVSTAKRLRGgvKFVDEVPKGLTGKIDRRKIRE 531
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
21-538 |
9.02e-49 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 177.32 E-value: 9.02e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 21 SPLSFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALER-VGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVC 99
Cdd:PRK12492 25 SVVEVFERSCKKFADRPAFSNLGVTLSYAELERHSAAFAAYLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVN 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 100 INIRLEARSIAFILRHCAARVLICDREFGAVAQQALA-----------MLDAPPL----LVGIDDDQAERA----DLAHD 160
Cdd:PRK12492 105 TNPLYTAREMRHQFKDSGARALVYLNMFGKLVQEVLPdtgieylieakMGDLLPAakgwLVNTVVDKVKKMvpayHLPQA 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 161 LDYEAFLAQGDPGRPLSAPQNEWQSIAINYTSGTTGDPKGVVLHHRGAYLN-----ACAGalifQLGPRSIYLWT----- 230
Cdd:PRK12492 185 VPFKQALRQGRGLSLKPVPVGLDDIAVLQYTGGTTGLAKGAMLTHGNLVANmlqvrACLS----QLGPDGQPLMKegqev 260
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 231 ----LPMFHCNGWSHTWAVTLSGGTHVCL----RKVqPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVSVIT 302
Cdd:PRK12492 261 miapLPLYHIYAFTANCMCMMVSGNHNVLitnpRDI-PGFIKELGKWRFSALLGLNTLFVALMDHPGFKDLDFSALKLTN 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 303 GGAappSAVIAAMEAR-----GFSITHAYGMTESygpstlclwQPGVDELPLEARAQFMSrqgVAHPMLEEATVLDTDTG 377
Cdd:PRK12492 340 SGG---TALVKATAERweqltGCTIVEGYGLTET---------SPVASTNPYGELARLGT---VGIPVPGTALKVIDDDG 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 378 RPVPADGRtlGELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEV 456
Cdd:PRK12492 405 NELPLGER--GELCIKGPQVMKGYWQQPEATAEALdAEGWFKTGDIAVIDPDGFVRIVDRKKDLIIVSGFNVYPNEIEDV 482
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 457 LYQHPEVVEAAVVARPDSRWGETPHAFVALRAdalPRTTGDDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKIQKFVLR 535
Cdd:PRK12492 483 VMAHPKVANCAAIGVPDERSGEAVKLFVVARD---PGLSVEELKAYCKENFTGYKVPKHIVLRDsLPMTPVGKILRRELR 559
|
...
gi 489250734 536 EWA 538
Cdd:PRK12492 560 DIA 562
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
47-535 |
1.09e-48 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 177.34 E-value: 1.09e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 47 SYRQLHERSCALASALERV-GVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDR 125
Cdd:PLN02574 68 SYSELQPLVKSMAAGLYHVmGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCSVGLAFTSP 147
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 126 EfgavAQQALAMLDAPPLLV----GIDDDQAERADLahdldYEAFLAQGDPG-RPlsaPQNEWQSIAINYTSGTTGDPKG 200
Cdd:PLN02574 148 E----NVEKLSPLGVPVIGVpenyDFDSKRIEFPKF-----YELIKEDFDFVpKP---VIKQDDVAAIMYSSGTTGASKG 215
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 201 VVLHHRgaylNACAGALIF---------QLGPRSIYLWTLPMFHCNGWSHTWAVTLS-GGTHVCLRKVQPDAIHAAIAEH 270
Cdd:PLN02574 216 VVLTHR----NLIAMVELFvrfeasqyeYPGSDNVYLAALPMFHIYGLSLFVVGLLSlGSTIVVMRRFDASDMVKVIDRF 291
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 271 AVTHLSAAPVVMSMLIHAEHASVPAVPVSV--ITGGAAPPSAVIAAMEARGFS---ITHAYGMTESYGPSTlclwqPGVD 345
Cdd:PLN02574 292 KVTHFPVVPPILMALTKKAKGVCGEVLKSLkqVSCGAAPLSGKFIQDFVQTLPhvdFIQGYGMTESTAVGT-----RGFN 366
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 346 ElpleARAQFMSRQGVAHPMLEeATVLDTDTGRPVPADGRtlGELVVRGNTVMKGYLHNPEATRA-ALADGWLHTGDLAV 424
Cdd:PLN02574 367 T----EKLSKYSSVGLLAPNMQ-AKVVDWSTGCLLPPGNC--GELWIQGPGVMKGYLNNPKATQStIDKDGWLRTGDIAY 439
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 425 LHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALprTTGDDLVRWCR 504
Cdd:PLN02574 440 FDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRQGST--LSQEAVINYVA 517
|
490 500 510
....*....|....*....|....*....|..
gi 489250734 505 ERLAHFKAPRHVTLTE-LPKTATGKIQKFVLR 535
Cdd:PLN02574 518 KQVAPYKKVRKVVFVQsIPKSPAGKILRRELK 549
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
2-535 |
1.11e-48 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 177.13 E-value: 1.11e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 2 MVPtleHELAPNEANHVPlsplSFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIP 81
Cdd:PRK07059 12 GVP---AEIDASQYPSLA----DLLEESFRQYADRPAFICMGKAITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVL 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 82 EMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDREFGAVAQQALAMLDAPPLLVGIDDD----------- 150
Cdd:PRK07059 85 QYPVAIAAVLRAGYVVVNVNPLYTPRELEHQLKDSGAEAIVVLENFATTVQQVLAKTAVKHVVVASMGDllgfkghivnf 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 151 -------QAERADLAHDLDYEAFLAQGDpGRPLSAPQNEWQSIA-INYTSGTTGDPKGVVLHHRGAYLNACAGAL----I 218
Cdd:PRK07059 165 vvrrvkkMVPAWSLPGHVRFNDALAEGA-RQTFKPVKLGPDDVAfLQYTGGTTGVSKGATLLHRNIVANVLQMEAwlqpA 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 219 FQLGPRS---IYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPDAIHAaiaehaVTHLSAAPVVM---------SMLI 286
Cdd:PRK07059 244 FEKKPRPdqlNFVCALPLYHIFALTVCGLLGMRTGGRNILIPNPRDIPGF------IKELKKYQVHIfpavntlynALLN 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 287 HAEHASVPAVPVSVITGGAAppsAVIAAMEARGFSITHAyGMTESYGPSTLclwQPGVDELPLEArAQFMSRQGVahPML 366
Cdd:PRK07059 318 NPDFDKLDFSKLIVANGGGM---AVQRPVAERWLEMTGC-PITEGYGLSET---SPVATCNPVDA-TEFSGTIGL--PLP 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 367 EEATVLDTDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEAT-RAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGG 445
Cdd:PRK07059 388 STEVSIRDDDGNDLPLG--EPGEICIRGPQVMAGYWNRPDETaKVMTADGFFRTGDVGVMDERGYTKIVDRKKDMILVSG 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 446 ENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVaLRADalPRTTGDDLVRWCRERLAHFKAPRHVTL-TELPKT 524
Cdd:PRK07059 466 FNVYPNEIEEVVASHPGVLEVAAVGVPDEHSGEAVKLFV-VKKD--PALTEEDVKAFCKERLTNYKRPKFVEFrTELPKT 542
|
570
....*....|.
gi 489250734 525 ATGKIQKFVLR 535
Cdd:PRK07059 543 NVGKILRRELR 553
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
25-536 |
1.12e-48 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 177.09 E-value: 1.12e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 25 FLKRAAQVYPQRDAVVYGA--RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINI 102
Cdd:PLN02330 33 FVLQDAELYADKVAFVEAVtgKAVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGGVFSGANP 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 103 RLEARSIAFILRHCAARVLIC-DREFGAVAQqalamLDAPPLLVGidddqaeRADLAHDLDYEAFLAQGDPGRPLSAPQN 181
Cdd:PLN02330 113 TALESEIKKQAEAAGAKLIVTnDTNYGKVKG-----LGLPVIVLG-------EEKIEGAVNWKELLEAADRAGDTSDNEE 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 182 EWQS--IAINYTSGTTGDPKGVVLHHRGAYLNACAGalIFQLGPRSI----YLWTLPMFHCNGWSHTWAVTL-SGGTHVC 254
Cdd:PLN02330 181 ILQTdlCALPFSSGTTGISKGVMLTHRNLVANLCSS--LFSVGPEMIgqvvTLGLIPFFHIYGITGICCATLrNKGKVVV 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 255 LRKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIH---AEHASVPAVPVSVITGGAAPPSA-VIAAMEAR--GFSITHAYGM 328
Cdd:PLN02330 259 MSRFELRTFLNALITQEVSFAPIVPPIILNLVKnpiVEEFDLSKLKLQAIMTAAAPLAPeLLTAFEAKfpGVQVQEAYGL 338
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 329 TEsYGPSTLCLWQPgvdelpleARAQFMSRQ---GVAHPMLEeATVLDTDTGRPVPADgrTLGELVVRGNTVMKGYLHNP 405
Cdd:PLN02330 339 TE-HSCITLTHGDP--------EKGHGIAKKnsvGFILPNLE-VKFIDPDTGRSLPKN--TPGELCVRSQCVMQGYYNNK 406
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 406 EAT-RAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFV 484
Cdd:PLN02330 407 EETdRTIDEDGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVPLPDEEAGEIPAACV 486
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 489250734 485 ALRADAlpRTTGDDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKIQKFVLRE 536
Cdd:PLN02330 487 VINPKA--KESEEDILNFVAANVAHYKKVRVVQFVDsIPKSLSGKIMRRLLKE 537
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
43-535 |
2.99e-48 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 173.39 E-value: 2.99e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 43 ARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLI 122
Cdd:cd05971 4 PEKVTFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 123 CDREfgavaqqalamlDAPPLLVgidddqaeradlahdldyeaflaqgdpgrplsapqnewqsiainYTSGTTGDPKGVV 202
Cdd:cd05971 84 TDGS------------DDPALII--------------------------------------------YTSGTTGPPKGAL 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 203 LHHRGAYLNACAGALIFQLGPRSIYL-WT--------------LPMFHcngwshtWAVTLSGGThvcLRKVQPDAIHAAI 267
Cdd:cd05971 108 HAHRVLLGHLPGVQFPFNLFPRDGDLyWTpadwawigglldvlLPSLY-------FGVPVLAHR---MTKFDPKAALDLM 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 268 AEHAVTHLSAAPVVMSML-IHAEHASVPAVPV-SVITGGAAPPSAVIA-AMEARGFSITHAYGMTESYGPSTLCL-WQPg 343
Cdd:cd05971 178 SRYGVTTAFLPPTALKMMrQQGEQLKHAQVKLrAIATGGESLGEELLGwAREQFGVEVNEFYGQTECNLVIGNCSaLFP- 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 344 vdelpleARAQFMSRQGVAHpmleEATVLDtDTGRPVPADGRtlGELVVR--GNTVMKGYLHNPEATRAALADGWLHTGD 421
Cdd:cd05971 257 -------IKPGSMGKPIPGH----RVAIVD-DNGTPLPPGEV--GEIAVElpDPVAFLGYWNNPSATEKKMAGDWLLTGD 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 422 LAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPrttGDDLVR 501
Cdd:cd05971 323 LGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVLNPGETP---SDALAR 399
|
490 500 510
....*....|....*....|....*....|....*....
gi 489250734 502 WCRE----RLAHFKAPRHVT-LTELPKTATGKIQKFVLR 535
Cdd:cd05971 400 EIQElvktRLAAHEYPREIEfVNELPRTATGKIRRRELR 438
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
190-535 |
4.34e-48 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 170.74 E-value: 4.34e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 190 YTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQP-------DA 262
Cdd:cd05944 9 HTGGTTGTPKLAQHTHSNEVYNAWMLALNSLFDPDDVLLCGLPLFHVNGSVVTLLTPLASGAHVVLAGPAGyrnpglfDN 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 263 IHAAIAEHAVTHLSAAPVVMSMLIhaehasvpAVPVS--------VITGGAAPPSAVIAAMEAR-GFSITHAYGMTESYG 333
Cdd:cd05944 89 FWKLVERYRITSLSTVPTVYAALL--------QVPVNadisslrfAMSGAAPLPVELRARFEDAtGLPVVEGYGLTEATC 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 334 PSTLCL----WQPGVDELPLEARAQFMSrqgvahpmleeatVLDTDTGRPVPADGRTLGELVVRGNTVMKGYLHNPEATR 409
Cdd:cd05944 161 LVAVNPpdgpKRPGSVGLRLPYARVRIK-------------VLDGVGRLLRDCAPDEVGEICVAGPGVFGGYLYTEGNKN 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 410 AALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRAD 489
Cdd:cd05944 228 AFVADGWLNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPG 307
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 489250734 490 AlpRTTGDDLVRWCRERLAHFKA-PRHVT-LTELPKTATGKIQKFVLR 535
Cdd:cd05944 308 A--VVEEEELLAWARDHVPERAAvPKHIEvLEELPVTAVGKVFKPALR 353
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
30-535 |
6.02e-48 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 174.04 E-value: 6.02e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 30 AQVYPQRDAVVYG--ARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEAR 107
Cdd:PRK13390 7 AQIAPDRPAVIVAetGEQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTAP 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 108 SIAFILRHCAARVLICDREFGAVAQQALAMLDAPPLLVGIDDDQAeradlahdlDYEAFLAQGDPgrplsaPQNEWQSIA 187
Cdd:PRK13390 87 EADYIVGDSGARVLVASAALDGLAAKVGADLPLRLSFGGEIDGFG---------SFEAALAGAGP------RLTEQPCGA 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 188 IN-YTSGTTGDPKGV-------VLHHRGAYLNACAGALiFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQ 259
Cdd:PRK13390 152 VMlYSSGTTGFPKGIqpdlpgrDVDAPGDPIVAIARAF-YDISESDIYYSSAPIYHAAPLRWCSMVHALGGTVVLAKRFD 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 260 PDAIHAAIAEHAVTHLSAAPVVMSMLI--HAEHASVPAVPV--SVITGGAAPPSAVIAAM-EARGFSITHAYGMTESYGP 334
Cdd:PRK13390 231 AQATLGHVERYRITVTQMVPTMFVRLLklDADVRTRYDVSSlrAVIHAAAPCPVDVKHAMiDWLGPIVYEYYSSTEAHGM 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 335 STLclwqpgvdelpleARAQFMSRQG-VAHPMLEEATVLDTDtGRPVPAdGRtLGELVVRGNTVMKGYLHNPEATRAALA 413
Cdd:PRK13390 311 TFI-------------DSPDWLAHPGsVGRSVLGDLHICDDD-GNELPA-GR-IGTVYFERDRLPFRYLNDPEKTAAAQH 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 414 DG---WLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADA 490
Cdd:PRK13390 375 PAhpfWTTVGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQVKAVIQLVEGI 454
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 489250734 491 LPRTT-GDDLVRWCRERLAHFKAPRHVTLT-ELPKTATGKIQKFVLR 535
Cdd:PRK13390 455 RGSDElARELIDYTRSRIAHYKAPRSVEFVdELPRTPTGKLVKGLLR 501
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
9-542 |
1.64e-47 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 174.18 E-value: 1.64e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 9 ELAPNEANHVPlsplSFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALER-VGVQAGERVAILAPNIPEMLEAH 87
Cdd:PRK05677 17 EINPDEYPNIQ----AVLKQSCQRFADKPAFSNLGKTLTYGELYKLSGAFAAWLQQhTDLKPGDRIAVQLPNVLQYPVAV 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 88 YGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDREFGAVAQQALAMLDAPPLLVgidddqAERADL---------- 157
Cdd:PRK05677 93 FGAMRAGLIVVNTNPLYTAREMEHQFNDSGAKALVCLANMAHLAEKVLPKTGVKHVIV------TEVADMlpplkrllin 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 158 -----------AHDL----DYEAFLAQGDpGRPLSAPQNEWQSIAI-NYTSGTTGDPKGVVLHHRG--AYLNACAGALIF 219
Cdd:PRK05677 167 avvkhvkkmvpAYHLpqavKFNDALAKGA-GQPVTEANPQADDVAVlQYTGGTTGVAKGAMLTHRNlvANMLQCRALMGS 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 220 QLGP-RSIYLWTLPMFHCNGWS-HTWAVTLSGGTHVCLRkvQPDAIHAAIAEHAVTHLSAApVVMSMLIHA-------EH 290
Cdd:PRK05677 246 NLNEgCEILIAPLPLYHIYAFTfHCMAMMLIGNHNILIS--NPRDLPAMVKELGKWKFSGF-VGLNTLFVAlcnneafRK 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 291 ASVPAVPVSvITGGAAPPSAViAAM--EARGFSITHAYGMTESygpstlclwQPGVDELPLEAraqfmsrqgvahpmLEE 368
Cdd:PRK05677 323 LDFSALKLT-LSGGMALQLAT-AERwkEVTGCAICEGYGMTET---------SPVVSVNPSQA--------------IQV 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 369 ATVldtdtGRPVPA--------DGRTL-----GELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGDLAVLHPDGYVEIK 434
Cdd:PRK05677 378 GTI-----GIPVPStlckviddDGNELplgevGELCVKGPQVMKGYWQRPEATDEILdSDGWLKTGDIALIQEDGYMRIV 452
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 435 DRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADalPRTTGDDLVRWCRERLAHFKAPR 514
Cdd:PRK05677 453 DRKKDMILVSGFNVYPNELEDVLAALPGVLQCAAIGVPDEKSGEAIKVFVVVKPG--ETLTKEQVMEHMRANLTGYKVPK 530
|
570 580
....*....|....*....|....*....
gi 489250734 515 HVTL-TELPKTATGKIqkfvLREWARQEE 542
Cdd:PRK05677 531 AVEFrDELPTTNVGKI----LRRELRDEE 555
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
30-529 |
2.92e-47 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 170.89 E-value: 2.92e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 30 AQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSI 109
Cdd:cd05945 1 AAANPDRPAVVEGGRTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAERI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 110 AFILRHCAarvlicdrefgavaqqalamldaPPLLVGIDDDQAeradlahdldyeaflaqgdpgrplsapqnewqsiAIN 189
Cdd:cd05945 81 REILDAAK-----------------------PALLIADGDDNA----------------------------------YII 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 190 YTSGTTGDPKGVVLHHRG--AYLNACAGAliFQLGPRSIYLWTLPmFHCNGWSHTWAVTL-SGGTHVCLRKVQ---PDAI 263
Cdd:cd05945 104 FTSGSTGRPKGVQISHDNlvSFTNWMLSD--FPLGPGDVFLNQAP-FSFDLSVMDLYPALaSGATLVPVPRDAtadPKQL 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 264 HAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVP--VSVITGGAAPPSAVIAAMEAR--GFSITHAYGMTESYGPSTLCL 339
Cdd:cd05945 181 FRFLAEHGITVWVSTPSFAAMCLLSPTFTPESLPslRHFLFCGEVLPHKTARALQQRfpDARIYNTYGPTEATVAVTYIE 260
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 340 WQP----GVDELPLearaqfmsrqGVAHPMLEeATVLDTDtGRPVPADGRtlGELVVRGNTVMKGYLHNPEATRAAL--A 413
Cdd:cd05945 261 VTPevldGYDRLPI----------GYAKPGAK-LVILDED-GRPVPPGEK--GELVISGPSVSKGYLNNPEKTAAAFfpD 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 414 DG--WLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAL 491
Cdd:cd05945 327 EGqrAYRTGDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALRQVPGVKEAVVVPKYKGEKVTELIAFVVPKPGAE 406
|
490 500 510
....*....|....*....|....*....|....*....
gi 489250734 492 PRTTgDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKI 529
Cdd:cd05945 407 AGLT-KAIKAELAERLPPYMIPRRfVYLDELPLNANGKI 444
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
26-529 |
3.06e-47 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 173.92 E-value: 3.06e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 26 LKRAAQVYPQRDAVVY------GARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVC 99
Cdd:cd17634 59 LDRHLRENGDRTAIIYegddtsQSRTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSV 138
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 100 INIRLEARSIAFILRHCAARVLICDREF---------GAVAQQALAMLDAPP--LLVGIDDDQAERADLAHDLDYEAFLA 168
Cdd:cd17634 139 IFGGFAPEAVAGRIIDSSSRLLITADGGvragrsvplKKNVDDALNPNVTSVehVIVLKRTGSDIDWQEGRDLWWRDLIA 218
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 169 QGDPgRPLSAPQNEWQSIAINYTSGTTGDPKGVVlHHRGAYLNACAGAL--IFQLGPRSIYLWTLPMfhcnGW--SHTWA 244
Cdd:cd17634 219 KASP-EHQPEAMNAEDPLFILYTSGTTGKPKGVL-HTTGGYLVYAATTMkyVFDYGPGDIYWCTADV----GWvtGHSYL 292
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 245 V---TLSGGTHVCLRKV----QPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVSV--ITGGAAPP------S 309
Cdd:cd17634 293 LygpLACGATTLLYEGVpnwpTPARMWQVVDKHGVNILYTAPTAIRALMAAGDDAIEGTDRSSlrILGSVGEPinpeayE 372
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 310 AVIAAMEARGFSITHAYGMTESYGpsTLCLWQPGVDelPLEARAQFMSRQGVAhpmleeATVLDTDtGRPVPadGRTLGE 389
Cdd:cd17634 373 WYWKKIGKEKCPVVDTWWQTETGG--FMITPLPGAI--ELKAGSATRPVFGVQ------PAVVDNE-GHPQP--GGTEGN 439
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 390 LVVR----GNTvmKGYLHNPE---ATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPE 462
Cdd:cd17634 440 LVITdpwpGQT--RTLFGDHErfeQTYFSTFKGMYFSGDGARRDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPK 517
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489250734 463 VVEAAVVARPDSRWGETPHAFVALRADALPRTT-GDDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKI 529
Cdd:cd17634 518 VAEAAVVGIPHAIKGQAPYAYVVLNHGVEPSPElYAELRNWVRKEIGPLATPDVVHWVDsLPKTRSGKI 586
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
21-544 |
5.78e-46 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 169.85 E-value: 5.78e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 21 SPLSFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALER-VGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVC 99
Cdd:PRK08974 24 SLVDMFEQAVARYADQPAFINMGEVMTFRKLEERSRAFAAYLQNgLGLKKGDRVALMMPNLLQYPIALFGILRAGMIVVN 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 100 INIRLEARSIAFILRHCAARVLICDREFGAVAQQALAMLDAPPLLVGIDDDQAERA-------------------DLAHD 160
Cdd:PRK08974 104 VNPLYTPRELEHQLNDSGAKAIVIVSNFAHTLEKVVFKTPVKHVILTRMGDQLSTAkgtlvnfvvkyikrlvpkyHLPDA 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 161 LDYEAFLAQGDpGRPLSAPQNEWQSIA-INYTSGTTGDPKGVVLHHRG--AYLNACAGALIFQLGPRSIYLWT-LPMFHc 236
Cdd:PRK08974 184 ISFRSALHKGR-RMQYVKPELVPEDLAfLQYTGGTTGVAKGAMLTHRNmlANLEQAKAAYGPLLHPGKELVVTaLPLYH- 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 237 ngwshTWAVTLS-------GGTHVC-------------LRKVQpdaihaaiaehaVTHLSAAPVVMSMLIHAE---HASV 293
Cdd:PRK08974 262 -----IFALTVNcllfielGGQNLLitnprdipgfvkeLKKYP------------FTAITGVNTLFNALLNNEefqELDF 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 294 PAVPVSViTGGAAPPSAVIAAMEA-RGFSITHAYGMTESygpstlclwQPGVDELPLEArAQFMSRQGVAHPMlEEATVL 372
Cdd:PRK08974 325 SSLKLSV-GGGMAVQQAVAERWVKlTGQYLLEGYGLTEC---------SPLVSVNPYDL-DYYSGSIGLPVPS-TEIKLV 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 373 DtDTGRPVPADGRtlGELVVRGNTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLE 452
Cdd:PRK08974 393 D-DDGNEVPPGEP--GELWVKGPQVMLGYWQRPEATDEVIKDGWLATGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNE 469
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 453 IEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALprtTGDDLVRWCRERLAHFKAPRHVTL-TELPKTATGKIQK 531
Cdd:PRK08974 470 IEDVVMLHPKVLEVAAVGVPSEVSGEAVKIFVVKKDPSL---TEEELITHCRRHLTGYKVPKLVEFrDELPKSNVGKILR 546
|
570
....*....|...
gi 489250734 532 FVLREwarqEEEA 544
Cdd:PRK08974 547 RELRD----EARA 555
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
53-529 |
7.39e-46 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 167.23 E-value: 7.39e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 53 ERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAArvlICDREFgAVAQ 132
Cdd:cd05922 1 LGVSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGGRLGLVFVPLNPTLKESVLRYLVA---DAGGRI-VLAD 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 133 QALAMLDAPPLLVGIDDDQAERAD-LAHDldyeaflaqgdpGRPLSAPQNEWQSIA-INYTSGTTGDPKGVVLHHRGAYL 210
Cdd:cd05922 77 AGAADRLRDALPASPDPGTVLDADgIRAA------------RASAPAHEVSHEDLAlLLYTSGSTGSPKLVRLSHQNLLA 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 211 NACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVC-LRKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIHAE 289
Cdd:cd05922 145 NARSIAEYLGITADDRALTVLPLSYDYGLSVLNTHLLRGATLVLtNDGVLDDAFWEDLREHGATGLAGVPSTYAMLTRLG 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 290 HASVPAVPVSVIT-GGAAPPSAVIAAMEA--RGFSITHAYGMTESYGPSTLclwqpgvdeLPLEaraQFMSRQGVAHPML 366
Cdd:cd05922 225 FDPAKLPSLRYLTqAGGRLPQETIARLREllPGAQVYVMYGQTEATRRMTY---------LPPE---RILEKPGSIGLAI 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 367 EEATVL-DTDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAA-LADGWLHTGDLAVLHPDGYVEIKDRAKDIIISG 444
Cdd:cd05922 293 PGGEFEiLDDDGTPTPPG--EPGEIVHRGPNVMKGYWNDPPYRRKEgRGGGVLHTGDLARRDEDGFLFIVGRRDRMIKLF 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 445 GENISSLEIEEVLYQHPEVVEAAVVARPDSRwGETPHAFVAlradALPRTTGDDLVRWCRERLAHFKAPRHVT-LTELPK 523
Cdd:cd05922 371 GNRISPTEIEAAARSIGLIIEAAAVGLPDPL-GEKLALFVT----APDKIDPKDVLRSLAERLPPYKVPATVRvVDELPL 445
|
....*.
gi 489250734 524 TATGKI 529
Cdd:cd05922 446 TASGKV 451
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
24-540 |
2.21e-45 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 167.61 E-value: 2.21e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 24 SFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIR 103
Cdd:PRK06164 14 SLLDAHARARPDAVALIDEDRPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIAVNTR 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 104 LEARSIAFILRHCAARVLICDREF---------GAVAQQALamLDAPPLLVGIDDDQAERADLAHDLDYEAFLAQGDPGR 174
Cdd:PRK06164 94 YRSHEVAHILGRGRARWLVVWPGFkgidfaailAAVPPDAL--PPLRAIAVVDDAADATPAPAPGARVQLFALPDPAPPA 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 175 PLSAPQNEWQSIAINY-TSGTTGDPKgVVLHHRGAYL-NACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTH 252
Cdd:PRK06164 172 AAGERAADPDAGALLFtTSGTTSGPK-LVLHRQATLLrHARAIARAYGYDPGAVLLAALPFCGVFGFSTLLGALAGGAPL 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 253 VCLRKVQPDAIHAAIAEHAVTHLSAAPVVMSmLIHAEHASVPAVPVSVITGGAAPPSA---VIAAMEARGFSITHAYGMT 329
Cdd:PRK06164 251 VCEPVFDAARTARALRRHRVTHTFGNDEMLR-RILDTAGERADFPSARLFGFASFAPAlgeLAALARARGVPLTGLYGSS 329
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 330 ESYGpstLCLWQPGvdELPLEARaqfmsRQGVAHPMLEEATV--LDTDTGRPVPaDGRTlGELVVRGNTVMKGYLHNPEA 407
Cdd:PRK06164 330 EVQA---LVALQPA--TDPVSVR-----IEGGGRPASPEARVraRDPQDGALLP-DGES-GEIEIRAPSLMRGYLDNPDA 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 408 TRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARpdSRWGET-PHAFVA 485
Cdd:PRK06164 398 TARALtDDGYFRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQVVGA--TRDGKTvPVAFVI 475
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 489250734 486 LRADAlpRTTGDDLVRWCRERLAHFKAPRHV-TLTELPKTATG---KIQKFVLREWARQ 540
Cdd:PRK06164 476 PTDGA--SPDEAGLMAACREALAGFKVPARVqVVEAFPVTESAngaKIQKHRLREMAQA 532
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
190-527 |
3.18e-45 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 162.09 E-value: 3.18e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 190 YTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPDAIHAAIAE 269
Cdd:cd17636 7 YTAAFSGRPNGALLSHQALLAQALVLAVLQAIDEGTVFLNSGPLFHIGTLMFTLATFHAGGTNVFVRRVDAEEVLELIEA 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 270 HAVTHLSAAPVVMSMLIHAEHASVPAVPVSVITGGAAPPSAVIAAMEARGFSITHAYGMTESYGPSTLcLWQPGvdelpl 349
Cdd:cd17636 87 ERCTHAFLLPPTIDQIVELNADGLYDLSSLRSSPAAPEWNDMATVDTSPWGRKPGGYGQTEVMGLATF-AALGG------ 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 350 earaQFMSRQGVAHPMLEeATVLDTDtGRPVPaDGRTlGELVVRGNTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDG 429
Cdd:cd17636 160 ----GAIGGAGRPSPLVQ-VRILDED-GREVP-DGEV-GEIVARGPTVMAGYWNRPEVNARRTRGGWHHTNDLGRREPDG 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 430 ---YVEIKDRakdIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAlpRTTGDDLVRWCRER 506
Cdd:cd17636 232 slsFVGPKTR---MIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWAQSVKAIVVLKPGA--SVTEAELIEHCRAR 306
|
330 340
....*....|....*....|..
gi 489250734 507 LAHFKAPRHVTLTE-LPKTATG 527
Cdd:cd17636 307 IASYKKPKSVEFADaLPRTAGG 328
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
44-537 |
8.69e-45 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 166.61 E-value: 8.69e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 44 RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVlVC----------INIRLEarsiafil 113
Cdd:PRK04319 72 EKYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPELYFALLGALKNGAI-VGplfeafmeeaVRDRLE-------- 142
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 114 rHCAARVLIC-DREFGAVAQQALAMLDAPpLLVGIDDDQAERadlahDLDYEAFLAQGDPGRPLSAPQNEWQSIaINYTS 192
Cdd:PRK04319 143 -DSEAKVLITtPALLERKPADDLPSLKHV-LLVGEDVEEGPG-----TLDFNALMEQASDEFDIEWTDREDGAI-LHYTS 214
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 193 GTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYlWtlpmfhCN---GWshtwaVT----------LSGGTHVCLR-KV 258
Cdd:PRK04319 215 GSTGKPKGVLHVHNAMLQHYQTGKYVLDLHEDDVY-W------CTadpGW-----VTgtsygifapwLNGATNVIDGgRF 282
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 259 QPDAIHAAIAEHAVTHLSAAPVVMSMLIHA--EHASVPAVP-----VSVitGGAAPPSAVIAAMEARGFSITHAYGMTES 331
Cdd:PRK04319 283 SPERWYRILEDYKVTVWYTAPTAIRMLMGAgdDLVKKYDLSslrhiLSV--GEPLNPEVVRWGMKVFGLPIHDNWWMTET 360
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 332 YG---PSTLCL-WQPGVDELPLEaraqfmsrqGVahpmleEATVLDtDTGRPVPADgrTLGELVVRGN--TVMKGYLHNP 405
Cdd:PRK04319 361 GGimiANYPAMdIKPGSMGKPLP---------GI------EAAIVD-DQGNELPPN--RMGNLAIKKGwpSMMRGIWNNP 422
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 406 EATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVA 485
Cdd:PRK04319 423 EKYESYFAGDWYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVAEAGVIGKPDPVRGEIIKAFVA 502
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 489250734 486 LRA-----DALPRttgdDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKIQKFVLREW 537
Cdd:PRK04319 503 LRPgyepsEELKE----EIRGFVKKGLGAHAAPREIEFKDkLPKTRSGKIMRRVLKAW 556
|
|
| benz_CoA_lig |
TIGR02262 |
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ... |
43-536 |
1.54e-44 |
|
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.
Pssm-ID: 274059 [Multi-domain] Cd Length: 505 Bit Score: 164.63 E-value: 1.54e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 43 ARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLI 122
Cdd:TIGR02262 28 ISSLSYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLGAIRAGIVPVALNTLLTADDYAYMLEDSRARVVF 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 123 CDREFGAVAQQALAMLDAPPLLVGIDDDQAERADLAhdldyeAFLAQGDPGRPLSAPQNE----WQsiainYTSGTTGDP 198
Cdd:TIGR02262 108 VSGALLPVIKAALGKSPHLEHRVVVGRPEAGEVQLA------ELLATESEQFKPAATQADdpafWL-----YSSGSTGMP 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 199 KGVVLHHRGAYLNA-CAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCL--RKVQPDAIHAAIAEHAVTHL 275
Cdd:TIGR02262 177 KGVVHTHSNPYWTAeLYARNTLGIREDDVCFSAAKLFFAYGLGNALTFPMSVGATTVLmgERPTPDAVFDRLRRHQPTIF 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 276 SAAPVVM-SMLIHAEHASVPAVPVSVIT-GGAAPPSAVIAAMEAR-GFSITHAYGMTEsygpstlclwqpgVDELPLEAR 352
Cdd:TIGR02262 257 YGVPTLYaAMLADPNLPSEDQVRLRLCTsAGEALPAEVGQRWQARfGVDIVDGIGSTE-------------MLHIFLSNL 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 353 AQFMSRQGVAHPMLEEATVLDTDTGRPVpADGRtLGELVVRGNTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVE 432
Cdd:TIGR02262 324 PGDVRYGTSGKPVPGYRLRLVGDGGQDV-ADGE-PGELLISGPSSATMYWNNRAKSRDTFQGEWTRSGDKYVRNDDGSYT 401
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 433 IKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAlpRTTGDDLVRWCRERLAHFKA 512
Cdd:TIGR02262 402 YAGRTDDMLKVSGIYVSPFEIESALIQHPAVLEAAVVGVADEDGLIKPKAFVVLRPGQ--TALETELKEHVKDRLAPYKY 479
|
490 500
....*....|....*....|....*
gi 489250734 513 PRHVTLT-ELPKTATGKIQKFVLRE 536
Cdd:TIGR02262 480 PRWIVFVdDLPKTATGKIQRFKLRE 504
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
45-545 |
1.62e-44 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 163.68 E-value: 1.62e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 45 RYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICD 124
Cdd:cd17640 5 RITYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVALVVE 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 125 RefgavaqqalamldappllvgiDDDqaeraDLAhdldyeaflaqgdpgrplsapqnewqsiAINYTSGTTGDPKGVVLH 204
Cdd:cd17640 85 N----------------------DSD-----DLA----------------------------TIIYTSGTTGNPKGVMLT 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 205 HRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVC---------LRKVQPdaihaaiaehavTHL 275
Cdd:cd17640 110 HANLLHQIRSLSDIVPPQPGDRFLSILPIWHSYERSAEYFIFACGCSQAYtsirtlkddLKRVKP------------HYI 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 276 SAAPVVMSML---IHAEHASVPAVP-------------VSVITGGAAPPSAVIAAMEARGFSITHAYGMTESyGPSTLC- 338
Cdd:cd17640 178 VSVPRLWESLysgIQKQVSKSSPIKqflflfflsggifKFGISGGGALPPHVDTFFEAIGIEVLNGYGLTET-SPVVSAr 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 339 -LWQPgvdelplearaqfmSRQGVAHPMLE-EATVLDTDTGRPVPADGRtlGELVVRGNTVMKGYLHNPEATRAAL-ADG 415
Cdd:cd17640 257 rLKCN--------------VRGSVGRPLPGtEIKIVDPEGNVVLPPGEK--GIVWVRGPQVMKGYYKNPEATSKVLdSDG 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 416 WLHTGDLAVLHPDGYVEIKDRAKD-IIISGGENISSLEIEEVLYQHPeVVEAAVVARPDSRwgetphafvALRADALPRT 494
Cdd:cd17640 321 WFNTGDLGWLTCGGELVLTGRAKDtIVLSNGENVEPQPIEEALMRSP-FIEQIMVVGQDQK---------RLGALIVPNF 390
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 489250734 495 tgDDLVRWCRERLAHFKAPRHVTLTELPKTA--TGKIQKFV-LREWARQEEEAG 545
Cdd:cd17640 391 --EELEKWAKESGVKLANDRSQLLASKKVLKlyKNEIKDEIsNRPGFKSFEQIA 442
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
30-544 |
3.98e-44 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 162.35 E-value: 3.98e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 30 AQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSI 109
Cdd:PRK09029 13 AQVRPQAIALRLNDEVLTWQQLCARIDQLAAGFAQQGVVEGSGVALRGKNSPETLLAYLALLQCGARVLPLNPQLPQPLL 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 110 AFILRHCAARVLICDREFGAVAqqalamldappllvGIDDDQAERADLAHDLDYeaflaqgDPGRPLSapqnewqsiaIN 189
Cdd:PRK09029 93 EELLPSLTLDFALVLEGENTFS--------------ALTSLHLQLVEGAHAVAW-------QPQRLAT----------MT 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 190 YTSGTTGDPKGVVlHHRGAYLNACAGAL-IFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVcLRKVQPdaihaaia 268
Cdd:PRK09029 142 LTSGSTGLPKAAV-HTAQAHLASAEGVLsLMPFTAQDSWLLSLPLFHVSGQGIVWRWLYAGATLV-VRDKQP-------- 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 269 ehavthLSAapvvmsMLIHAEHAS-VP----------AVPVS---VITGGAAPPSAVIAAMEARGFSITHAYGMTESygP 334
Cdd:PRK09029 212 ------LEQ------ALAGCTHASlVPtqlwrlldnrSEPLSlkaVLLGGAAIPVELTEQAEQQGIRCWCGYGLTEM--A 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 335 STLCLWQpgVDELPlearaqfmsrqGVahpmleeatvldtdtGRPVPadGRTL----GELVVRGNTVMKGYLHNPEATRA 410
Cdd:PRK09029 278 STVCAKR--ADGLA-----------GV---------------GSPLP--GREVklvdGEIWLRGASLALGYWRQGQLVPL 327
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 411 ALADGWLHTGDLAVLHpDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADA 490
Cdd:PRK09029 328 VNDEGWFATRDRGEWQ-NGELTILGRLDNLFFSGGEGIQPEEIERVINQHPLVQQVFVVPVADAEFGQRPVAVVESDSEA 406
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 489250734 491 LPrttgDDLVRWCRERLAHFKAP-RHVTLTELPKTATGKIQKFVLREWARQEEEA 544
Cdd:PRK09029 407 AV----VNLAEWLQDKLARFQQPvAYYLLPPELKNGGIKISRQALKEWVAQQLGN 457
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
25-536 |
1.40e-43 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 161.73 E-value: 1.40e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 25 FLKRAAQVyPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRL 104
Cdd:cd17655 3 FEEQAEKT-PDHTAVVFEDQTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDPDY 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 105 EARSIAFILRHCAARVLicdrefgaVAQQALAMLDAPPLLVGIDDDQAERADLAHDLdyeaflaqgdpgrplsAPQNEWQ 184
Cdd:cd17655 82 PEERIQYILEDSGADIL--------LTQSHLQPPIAFIGLIDLLDEDTIYHEESENL----------------EPVSKSD 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 185 SIA-INYTSGTTGDPKGVVLHHRGA---------YLNACAGALIFQLGPRSIYLWTLPMFHcngwshtwaVTLSGGTHVC 254
Cdd:cd17655 138 DLAyVIYTSGSTGKPKGVMIEHRGVvnlvewankVIYQGEHLRVALFASISFDASVTEIFA---------SLLSGNTLYI 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 255 LRKVQ---PDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVSVITGGAAPPSAV--IAAMEARGFSITHAYGMT 329
Cdd:cd17655 209 VRKETvldGQALTQYIRQNRITIIDLTPAHLKLLDAADDSEGLSLKHLIVGGEALSTELAkkIIELFGTNPTITNAYGPT 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 330 ESYGPSTLCLWQPGVDE---LPLEARAQFMSrqgvahpmleeATVLDTDtGRPVPADgrTLGELVVRGNTVMKGYLHNPE 406
Cdd:cd17655 289 ETTVDASIYQYEPETDQqvsVPIGKPLGNTR-----------IYILDQY-GRPQPVG--VAGELYIGGEGVARGYLNRPE 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 407 ATRAALAD------GWLH-TGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGET 479
Cdd:cd17655 355 LTAEKFVDdpfvpgERMYrTGDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEIEARLLQHPDIKEAVVIARKDEQGQNY 434
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 489250734 480 PHAFVALRADAlprtTGDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQKFVLRE 536
Cdd:cd17655 435 LCAYIVSEKEL----PVAQLREFLARELPDYMIPSYfIKLDEIPLTPNGKVDRKALPE 488
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
28-529 |
3.16e-43 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 160.52 E-value: 3.16e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 28 RAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEAR 107
Cdd:cd17646 6 EQAARTPDAPAVVDEGRTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLDPGYPAD 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 108 SIAFILRHCAARVLICDREfgavaqqalamlDAPPLLVGIDDDQAERADLAHdldyeaflAQGDPGRPLSAPQNEWQSIa 187
Cdd:cd17646 86 RLAYMLADAGPAVVLTTAD------------LAARLPAGGDVALLGDEALAA--------PPATPPLVPPRPDNLAYVI- 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 188 inYTSGTTGDPKGVVLHHRGA---YLNACAGaliFQLGPRSIYLWTLPM-FHCNGWSHTWAVtLSGGTHVCLR---KVQP 260
Cdd:cd17646 145 --YTSGSTGRPKGVMVTHAGIvnrLLWMQDE---YPLGPGDRVLQKTPLsFDVSVWELFWPL-VAGARLVVARpggHRDP 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 261 DAIHAAIAEHAVThlsAAPVVMSMLIHA-------EHASVPAVPVSvitGGAAPPSAVIAAMEARGFSITHAYGMTESYG 333
Cdd:cd17646 219 AYLAALIREHGVT---TCHFVPSMLRVFlaepaagSCASLRRVFCS---GEALPPELAARFLALPGAELHNLYGPTEAAI 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 334 PSTLCLWQPGVDELPLEaraqfmsrqgVAHPMLE-EATVLDtDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAAL 412
Cdd:cd17646 293 DVTHWPVRGPAETPSVP----------IGRPVPNtRLYVLD-DALRPVPVG--VPGELYLGGVQLARGYLGRPALTAERF 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 413 ADGWL-------HTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVA 485
Cdd:cd17646 360 VPDPFgpgsrmyRTGDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHPAVTHAVVVARAAPAGAARLVGYVV 439
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 489250734 486 LRADALPRTTgDDLVRWCRERLAHFKAP-RHVTLTELPKTATGKI 529
Cdd:cd17646 440 PAAGAAGPDT-AALRAHLAERLPEYMVPaAFVVLDALPLTANGKL 483
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
47-468 |
4.40e-43 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 158.58 E-value: 4.40e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 47 SYRQLHERSCALASAL-ERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDR 125
Cdd:TIGR01733 1 TYRELDERANRLARHLrAAGGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDS 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 126 EFgavaQQALAMLDAPPLLVGIDddqaeradlahDLDYEAFLAQGDPGRPLSAPQNewqsIA-INYTSGTTGDPKGVVLH 204
Cdd:TIGR01733 81 AL----ASRLAGLVLPVILLDPL-----------ELAALDDAPAPPPPDAPSGPDD----LAyVIYTSGSTGRPKGVVVT 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 205 HRGAyLNACAGAL-IFQLGPRSIYLWTLPmFHCNGWSHTWAVTLSGGTHVCL-----RKVQPDAIHAAIAEHAVTHLSAA 278
Cdd:TIGR01733 142 HRSL-VNLLAWLArRYGLDPDDRVLQFAS-LSFDASVEEIFGALLAGATLVVppedeERDDAALLAALIAEHPVTVLNLT 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 279 PVVMSMLIHAEHASVPAVPVsVITGGAAPPSAVIAAMEAR--GFSITHAYGMTESYGPSTLCLWQPGVDELPlearaqfm 356
Cdd:TIGR01733 220 PSLLALLAAALPPALASLRL-VILGGEALTPALVDRWRARgpGARLINLYGPTETTVWSTATLVDPDDAPRE-------- 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 357 SRQGVAHPMLE-EATVLDtDTGRPVPADGrtLGELVVRGNTVMKGYLHNPEATRAALADG---------WLHTGDLAVLH 426
Cdd:TIGR01733 291 SPVPIGRPLANtRLYVLD-DDLRPVPVGV--VGELYIGGPGVARGYLNRPELTAERFVPDpfaggdgarLYRTGDLVRYL 367
|
410 420 430 440
....*....|....*....|....*....|....*....|..
gi 489250734 427 PDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAV 468
Cdd:TIGR01733 368 PDGNLEFLGRIDDQVKIRGYRIELGEIEAALLRHPGVREAVV 409
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
28-536 |
7.57e-43 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 160.87 E-value: 7.57e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 28 RAAQVYPQRDAVVY------GARRYSYRQLHERSCALASALERVGvQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCI- 100
Cdd:cd05931 1 RRAAARPDRPAYTFlddeggREETLTYAELDRRARAIAARLQAVG-KPGDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLp 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 101 --NIRLEARSIAFILRHCAARVLICDREFGAVAQQALAMLDAPPLLVGIDDDQAEradlahdldyeaflaqGDPGRPLSA 178
Cdd:cd05931 80 ppTPGRHAERLAAILADAGPRVVLTTAAALAAVRAFAASRPAAGTPRLLVVDLLP----------------DTSAADWPP 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 179 PQNEWQSIA-INYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFH------------CNGWSHTW-- 243
Cdd:cd05931 144 PSPDPDDIAyLQYTSGSTGTPKGVVVTHRNLLANVRQIRRAYGLDPGDVVVSWLPLYHdmgligglltplYSGGPSVLms 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 244 -------------AVTLSGGTH---------VCLRKVQPDaihaaiaehavthlSAAPVVMSmliHAEHASVPAVPVSvi 301
Cdd:cd05931 224 paaflrrplrwlrLISRYRATIsaapnfaydLCVRRVRDE--------------DLEGLDLS---SWRVALNGAEPVR-- 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 302 tggAAPPSAVIAAMEARGF---SITHAYGMTE-----SYGPSTLCLWQPGVDELPLEARAQFMSRQG-----------VA 362
Cdd:cd05931 285 ---PATLRRFAEAFAPFGFrpeAFRPSYGLAEatlfvSGGPPGTGPVVLRVDRDALAGRAVAVAADDpaarelvscgrPL 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 363 HPMleEATVLDTDTGRPVPAdgRTLGELVVRGNTVMKGYLHNPEATRAAL-------ADGWLHTGDLAVLHpDGYVEIKD 435
Cdd:cd05931 362 PDQ--EVRIVDPETGRELPD--GEVGEIWVRGPSVASGYWGRPEATAETFgalaatdEGGWLRTGDLGFLH-DGELYITG 436
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 436 RAKDIIISGGENISSLEIEEVLYQ-HPEVVEAAVVARPDSRWGETPHAFVALRADALPRTTGDDLVRWCRERLA--HFKA 512
Cdd:cd05931 437 RLKDLIIVRGRNHYPQDIEATAEEaHPALRPGCVAAFSVPDDGEERLVVVAEVERGADPADLAAIAAAIRAAVAreHGVA 516
|
570 580
....*....|....*....|....*..
gi 489250734 513 PRHVTLTE---LPKTATGKIQKFVLRE 536
Cdd:cd05931 517 PADVVLVRpgsIPRTSSGKIQRRACRA 543
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
26-536 |
1.12e-42 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 159.97 E-value: 1.12e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 26 LKRAAQVYPQRDAVV----YGARR-YSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCI 100
Cdd:cd05970 23 VDAMAKEYPDKLALVwcddAGEERiFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLALHKLGAIAIPA 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 101 NIRLEARSIAFILRHCAARVLICDREFGA--VAQQALAMLDAPPLLVGIDDDQAERAdlahdLDYEAFLAQGDPG--RPL 176
Cdd:cd05970 103 THQLTAKDIVYRIESADIKMIVAIAEDNIpeEIEKAAPECPSKPKLVWVGDPVPEGW-----IDFRKLIKNASPDfeRPT 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 177 SAPQNEWQSIAINY-TSGTTGDPKGVvlHHRGAY-LNACAGALIFQ-LGPRSIYL------WTLPMfhcngWSHTWAVTL 247
Cdd:cd05970 178 ANSYPCGEDILLVYfSSGTTGMPKMV--EHDFTYpLGHIVTAKYWQnVREGGLHLtvadtgWGKAV-----WGKIYGQWI 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 248 SGGTHVC--LRKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIHA--EHASVPAVPVSVITGGAAPPSAVIAAMEARGFSIT 323
Cdd:cd05970 251 AGAAVFVydYDKFDPKALLEKLSKYGVTTFCAPPTIYRFLIREdlSRYDLSSLRYCTTAGEALNPEVFNTFKEKTGIKLM 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 324 HAYGMTESygpsTLClwqpgVDELP-LEARAQFMSRQGVAHPMleeaTVLDTDtGRPVPADGRtlGELVVRGNT-----V 397
Cdd:cd05970 331 EGFGQTET----TLT-----IATFPwMEPKPGSMGKPAPGYEI----DLIDRE-GRSCEAGEE--GEIVIRTSKgkpvgL 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 398 MKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWG 477
Cdd:cd05970 395 FGGYYKDAEKTAEVWHDGYYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQHPAVLECAVTGVPDPIRG 474
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 489250734 478 ETPHAFVALRADALPrttGDDLVR----WCRERLAHFKAPRHVTLT-ELPKTATGKIQKFVLRE 536
Cdd:cd05970 475 QVVKATIVLAKGYEP---SEELKKelqdHVKKVTAPYKYPRIVEFVdELPKTISGKIRRVEIRE 535
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
79-536 |
1.98e-42 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 158.31 E-value: 1.98e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 79 NIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLIC--DREFgavaqQALAMLDAPPLLVGIDDDQAERAD 156
Cdd:cd05929 31 ARAAAAEGVWIADGVYIYLINSILTVFAAAAAWKCGACPAYKSSRapRAEA-----CAIIEIKAAALVCGLFTGGGALDG 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 157 LAhdlDYEAFLAqgdpGRPLSAPQNEWQSIAINYTSGTTGDPKGVVLHHRGAYLNACAG---ALIFQLGPRSIYLWTLPM 233
Cdd:cd05929 106 LE---DYEAAEG----GSPETPIEDEAAGWKMLYSGGTTGRPKGIKRGLPGGPPDNDTLmaaALGFGPGADSVYLSPAPL 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 234 FHcnGWSHTWAVT--LSGGTHVCLRKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVS----VITGGAAP 307
Cdd:cd05929 179 YH--AAPFRWSMTalFMGGTLVLMEKFDPEEFLRLIERYRVTFAQFVPTMFVRLLKLPEAVRNAYDLSslkrVIHAAAPC 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 308 PSAVIAAMEARGFSITHA-YGMTESYGpSTLClwqpgvdelpleARAQFMSRQG-VAHPMLEEATVLDTDtGRPVPAdgR 385
Cdd:cd05929 257 PPWVKEQWIDWGGPIIWEyYGGTEGQG-LTII------------NGEEWLTHPGsVGRAVLGKVHILDED-GNEVPP--G 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 386 TLGELVVRGNTVmKGYLHNPEATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVV 464
Cdd:cd05929 321 EIGEVYFANGPG-FEYTNDPEKTAAARnEGGWSTLGDVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVL 399
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 489250734 465 EAAVVARPDSRWGETPHAFVALRADALPRTT-GDDLVRWCRERLAHFKAPRHVTL-TELPKTATGKIQKFVLRE 536
Cdd:cd05929 400 DAAVVGVPDEELGQRVHAVVQPAPGADAGTAlAEELIAFLRDRLSRYKCPRSIEFvAELPRDDTGKLYRRLLRD 473
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
29-544 |
2.71e-42 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 159.00 E-value: 2.71e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 29 AAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVciNI-----R 103
Cdd:PRK10946 32 TRHAASDAIAVICGERQFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLGVAPV--NAlfshqR 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 104 LEARSIAfilRHCAARVLICDREFGAVAQQAlaMLDA-----PPLLVGIDDDQAERADLAHDLDYEAflaqgDPGRPLSA 178
Cdd:PRK10946 110 SELNAYA---SQIEPALLIADRQHALFSDDD--FLNTlvaehSSLRVVLLLNDDGEHSLDDAINHPA-----EDFTATPS 179
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 179 PQNEwqsIAINYTSG-TTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWA--VTLSGGTHVCL 255
Cdd:PRK10946 180 PADE---VAFFQLSGgSTGTPKLIPRTHNDYYYSVRRSVEICGFTPQTRYLCALPAAHNYPMSSPGAlgVFLAGGTVVLA 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 256 RKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHAS---VPAVPVSVITGGAAPPSAVIAAM--EARGFSITHAYGMTE 330
Cdd:PRK10946 257 PDPSATLCFPLIEKHQVNVTALVPPAVSLWLQAIAEGgsrAQLASLKLLQVGGARLSETLARRipAELGCQLQQVFGMAE 336
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 331 SYGPSTlclwqpGVDELPlearAQFMSRQGvaHPML--EEATVLDTDtGRPVPaDGRTlGELVVRGNTVMKGYLHNPEAT 408
Cdd:PRK10946 337 GLVNYT------RLDDSD----ERIFTTQG--RPMSpdDEVWVADAD-GNPLP-QGEV-GRLMTRGPYTFRGYYKSPQHN 401
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 409 RAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALR 487
Cdd:PRK10946 402 ASAFdANGFYCSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGEKSCAFLVVK 481
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 489250734 488 ADALPRTtgddLVRWCRER-LAHFKAPRHV-TLTELPKTATGKIQKFVLREWARQEEEA 544
Cdd:PRK10946 482 EPLKAVQ----LRRFLREQgIAEFKLPDRVeCVDSLPLTAVGKVDKKQLRQWLASRASA 536
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
34-529 |
3.10e-42 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 157.45 E-value: 3.10e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 34 PQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFIL 113
Cdd:cd12116 1 PDATAVRDDDRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLRYIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 114 RHCAARVLICDRefgavaqqalAMLDAPPLLVGIDDDQAERADLAHDldyeaflaqgDPGRPLSAPqnewQSIAINYTSG 193
Cdd:cd12116 81 EDAEPALVLTDD----------ALPDRLPAGLPVLLLALAAAAAAPA----------APRTPVSPD----DLAYVIYTSG 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 194 TTGDPKGVVLHHRG--AYLNACAGALifQLGPRSIYLW-TLPMFHCNGWSHTWAVtLSGGT-HVCLRKVQPDAIHAAIA- 268
Cdd:cd12116 137 STGRPKGVVVSHRNlvNFLHSMRERL--GLGPGDRLLAvTTYAFDISLLELLLPL-LAGARvVIAPRETQRDPEALARLi 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 269 -EHAVTHLSAAPVVMSMLIHAEHAsvPAVPVSVITGGAAPPSAVIAAMEARGFSITHAYGMTESYGPSTLCLWQPGVDEL 347
Cdd:cd12116 214 eAHSITVMQATPATWRMLLDAGWQ--GRAGLTALCGGEALPPDLAARLLSRVGSLWNLYGPTETTIWSTAARVTAAAGPI 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 348 PLearaqfmsrqgvAHPMLE-EATVLDTDtGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAALADG--------WLH 418
Cdd:cd12116 292 PI------------GRPLANtQVYVLDAA-LRPVPPG--VPGELYIGGDGVAQGYLGRPALTAERFVPDpfagpgsrLYR 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 419 TGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDsrwGETPH--AFVALRADALPRTtg 496
Cdd:cd12116 357 TGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEIEAALAAHPGVAQAAVVVRED---GGDRRlvAYVVLKAGAAPDA-- 431
|
490 500 510
....*....|....*....|....*....|....
gi 489250734 497 DDLVRWCRERLAHFKAPRH-VTLTELPKTATGKI 529
Cdd:cd12116 432 AALRAHLRATLPAYMVPSAfVRLDALPLTANGKL 465
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
46-535 |
3.97e-42 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 156.52 E-value: 3.97e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 46 YSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDr 125
Cdd:cd05973 1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVVTD- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 126 efgavaqqalamldappllvgidddqaerADLAHDLDYEAFLAQgdpgrplsapqnewqsiainYTSGTTGDPKGVVLHH 205
Cdd:cd05973 80 -----------------------------AANRHKLDSDPFVMM--------------------FTSGTTGLPKGVPVPL 110
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 206 RgaYLNACAGALIFQLG--PRSIYlWTL--PmfhcnGWSHT--WAVT--LSGGTHVCLRK--VQPDAIHAAIAEHAVTHL 275
Cdd:cd05973 111 R--ALAAFGAYLRDAVDlrPEDSF-WNAadP-----GWAYGlyYAITgpLALGHPTILLEggFSVESTWRVIERLGVTNL 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 276 SAAPVVMSMLIhAEHASVPAVP---VSVITGGAAP--PSAVIAAMEARGFSITHAYGMTESygpsTLCLWQPGVDELPLE 350
Cdd:cd05973 183 AGSPTAYRLLM-AAGAEVPARPkgrLRRVSSAGEPltPEVIRWFDAALGVPIHDHYGQTEL----GMVLANHHALEHPVH 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 351 ARAQfmsrqGVAHPMLEeATVLDTDTGRPVPadgRTLGELV--VRGNTVM--KGYLHNPEAtraALADGWLHTGDLAVLH 426
Cdd:cd05973 258 AGSA-----GRAMPGWR-VAVLDDDGDELGP---GEPGRLAidIANSPLMwfRGYQLPDTP---AIDGGYYLTGDTVEFD 325
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 427 PDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPRTT-GDDLVRWCRE 505
Cdd:cd05973 326 PDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHEGTPAlADELQLHVKK 405
|
490 500 510
....*....|....*....|....*....|.
gi 489250734 506 RLAHFKAPRHVT-LTELPKTATGKIQKFVLR 535
Cdd:cd05973 406 RLSAHAYPRTIHfVDELPKTPSGKIQRFLLR 436
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
28-529 |
5.24e-42 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 157.51 E-value: 5.24e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 28 RAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEAR 107
Cdd:cd17651 3 RQAARTPDAPALVAEGRRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYPAE 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 108 SIAFILRHCAARVLicdrefgaVAQQALAMLDAPPLLVGIDDDQAEradlahdldyeafLAQGDPGRPLSAPQNEWQSIA 187
Cdd:cd17651 83 RLAFMLADAGPVLV--------LTHPALAGELAVELVAVTLLDQPG-------------AAAGADAEPDPALDADDLAYV 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 188 InYTSGTTGDPKGVVLHHRG---------AYLNACAGALIFQLGPRS--IYLWTLpmfhcngWShtwavTLSGGTHVCLR 256
Cdd:cd17651 142 I-YTSGSTGRPKGVVMPHRSlanlvawqaRASSLGPGARTLQFAGLGfdVSVQEI-------FS-----TLCAGATLVLP 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 257 ----KVQPDAIHAAIAEHAVTHLSAAPVVMSMLI-HAEHASV--PAVPVSVITGGAAPPSAVIAAMEAR--GFSITHAYG 327
Cdd:cd17651 209 peevRTDPPALAAWLDEQRISRVFLPTVALRALAeHGRPLGVrlAALRYLLTGGEQLVLTEDLREFCAGlpGLRLHNHYG 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 328 MTESYgpSTLCLWQPGvdelpleARAQFMSRQGVAHPMLE-EATVLDTDtGRPVPaDGRTlGELVVRGNTVMKGYLHNPE 406
Cdd:cd17651 289 PTETH--VVTALSLPG-------DPAAWPAPPPIGRPIDNtRVYVLDAA-LRPVP-PGVP-GELYIGGAGLARGYLNRPE 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 407 ATRAA-LADGWL------HTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGET 479
Cdd:cd17651 357 LTAERfVPDPFVpgarmyRTGDLARWLPDGELEFLGRADDQVKIRGFRIELGEIEAALARHPGVREAVVLAREDRPGEKR 436
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 489250734 480 PHAFVALRADALPRTTgdDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKI 529
Cdd:cd17651 437 LVAYVVGDPEAPVDAA--ELRAALATHLPEYMVPSAFVLLDaLPLTPNGKL 485
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
34-531 |
3.02e-40 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 151.69 E-value: 3.02e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 34 PQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFIL 113
Cdd:cd17643 1 PEAVAVVDEDRRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVERIAFIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 114 RHCAARVLICDrefgavaqqalamldappllvgidddqaeradlAHDLDYeaflaqgdpgrplsapqnewqsiaINYTSG 193
Cdd:cd17643 81 ADSGPSLLLTD---------------------------------PDDLAY------------------------VIYTSG 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 194 TTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIylWTlpMFHCNG-----WsHTWAVTLSGGTHVCLRKVQ---PDAIHA 265
Cdd:cd17643 104 STGRPKGVVVSHANVLALFAATQRWFGFNEDDV--WT--LFHSYAfdfsvW-EIWGALLHGGRLVVVPYEVarsPEDFAR 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 266 AIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPV--SVITGGAAPPSAVIA----AMEARGFSITHAYGMTESYGPSTlcl 339
Cdd:cd17643 179 LLRDEGVTVLNQTPSAFYQLVEAADRDGRDPLAlrYVIFGGEALEAAMLRpwagRFGLDRPQLVNMYGITETTVHVT--- 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 340 WQPgVDELPLEARAqfMSRQGVAHPMLeEATVLDTDtGRPVPADGrtLGELVVRGNTVMKGYLHNPE--ATR----AALA 413
Cdd:cd17643 256 FRP-LDAADLPAAA--ASPIGRPLPGL-RVYVLDAD-GRPVPPGV--VGELYVSGAGVARGYLGRPEltAERfvanPFGG 328
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 414 DG--WLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAL 491
Cdd:cd17643 329 PGsrMYRTGDLARRLPDGELEYLGRADEQVKIRGFRIELGEIEAALATHPSVRDAAVIVREDEPGDTRLVAYVVADDGAA 408
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 489250734 492 PRTTgdDLVRWCRERLAHFKAP-RHVTLTELPKTATGKIQK 531
Cdd:cd17643 409 ADIA--ELRALLKELLPDYMVPaRYVPLDALPLTVNGKLDR 447
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
47-538 |
7.57e-40 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 151.33 E-value: 7.57e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 47 SYRQLHERSCALASALERvGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDRE 126
Cdd:cd05909 9 TYRKLLTGAIALARKLAK-MTKEGENVGVMLPPSAGGALANFALALSGKVPVMLNYTAGLRELRACIKLAGIKTVLTSKQ 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 127 F-GAVAQQALAMLDAPPLLVGIDDDQAE-------RADLA-----HDLDYEAFLAQGDPGRPLsapqnewqsiAINYTSG 193
Cdd:cd05909 88 FiEKLKLHHLFDVEYDARIVYLEDLRAKiskadkcKAFLAgkfppKWLLRIFGVAPVQPDDPA----------VILFTSG 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 194 TTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHT-WAVTLSGGTHVC------LRKVQPdaihaA 266
Cdd:cd05909 158 SEGLPKGVVLSHKNLLANVEQITAIFDPNPEDVVFGALPFFHSFGLTGClWLPLLSGIKVVFhpnpldYKKIPE-----L 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 267 IAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVSVITGGAAPPSAVI-AAMEARGFSITHAYGMTESYgpstlclwqpgvd 345
Cdd:cd05909 233 IYDKKATILLGTPTFLRGYARAAHPEDFSSLRLVVAGAEKLKDTLRqEFQEKFGIRILEGYGTTECS------------- 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 346 elPLEA--RAQFMSRQG-VAHPM-LEEATVLDTDTGRPVPAdGRTlGELVVRGNTVMKGYLHNPEATRAALADGWLHTGD 421
Cdd:cd05909 300 --PVISvnTPQSPNKEGtVGRPLpGMEVKIVSVETHEEVPI-GEG-GLLLVRGPNVMLGYLNEPELTSFAFGDGWYDTGD 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 422 LAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQH-PEVVEAAVVARPDSRWGETPHAFValradALPRTTGDDLV 500
Cdd:cd05909 376 IGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSEIlPEDNEVAVVSVPDGRKGEKIVLLT-----TTTDTDPSSLN 450
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 489250734 501 RWCRE-RLAHFKAPRHV-TLTELPKTATGKIQKFVLREWA 538
Cdd:cd05909 451 DILKNaGISNLAKPSYIhQVEEIPLLGTGKPDYVTLKALA 490
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
36-536 |
2.02e-39 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 150.99 E-value: 2.02e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 36 RDAVVYGARRYSYRQLHERSCALASAL-ERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILR 114
Cdd:PRK07867 19 DRGLYFEDSFTSWREHIRGSAARAAALrARLDPTRPPHVGVLLDNTPEFSLLLGAAALSGIVPVGLNPTRRGAALARDIA 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 115 HCAARVLICDREFGAVAQqalamlDAPPLLVGIDDDQAERADLAhdldyeAFLAQGDPGRPLSAPQNEWQSIainYTSGT 194
Cdd:PRK07867 99 HADCQLVLTESAHAELLD------GLDPGVRVINVDSPAWADEL------AAHRDAEPPFRVADPDDLFMLI---FTSGT 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 195 TGDPKGVVLHHRGAylnACAGALI---FQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLR-KVQPDAIHAAIAEH 270
Cdd:PRK07867 164 SGDPKAVRCTHRKV---ASAGVMLaqrFGLGPDDVCYVSMPLFHSNAVMAGWAVALAAGASIALRrKFSASGFLPDVRRY 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 271 AVTHLSAAPVVMSMLIHAEHASVPA-VPVSVITGGAAPPSAvIAAMEAR-GFSITHAYGMTESyGPSTLclWQPGvdelp 348
Cdd:PRK07867 241 GATYANYVGKPLSYVLATPERPDDAdNPLRIVYGNEGAPGD-IARFARRfGCVVVDGFGSTEG-GVAIT--RTPD----- 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 349 learaqfmSRQGVAHPMLEEATVLDTDTGRPVP-----ADGRT-----LGELV-VRGNTVMKGYLHNPEATRAALADGWL 417
Cdd:PRK07867 312 --------TPPGALGPLPPGVAIVDPDTGTECPpaedaDGRLLnadeaIGELVnTAGPGGFEGYYNDPEADAERMRGGVY 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 418 HTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAL--PRTT 495
Cdd:PRK07867 384 WSGDLAYRDADGYAYFAGRLGDWMRVDGENLGTAPIERILLRYPDATEVAVYAVPDPVVGDQVMAALVLAPGAKfdPDAF 463
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 489250734 496 GDDLVrwCRERLAHFKAPRHV-TLTELPKTATGKIQKFVLRE 536
Cdd:PRK07867 464 AEFLA--AQPDLGPKQWPSYVrVCAELPRTATFKVLKRQLSA 503
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
27-543 |
3.45e-39 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 149.54 E-value: 3.45e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 27 KRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALervGVQAGE--RVAILAPNIPEMLEAHYGVPGAGAVLVCINIRL 104
Cdd:PRK07638 8 KKHASLQPNKIAIKENDRVLTYKDWFESVCKVANWL---NEKESKnkTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKW 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 105 EARSIAFILRHCAARVLICDREFgavaqqaLAMLDAPPLLVgIDDDQAERadlahdlDYEAFLAQGDPGRPLsapqnEWQ 184
Cdd:PRK07638 85 KQDELKERLAISNADMIVTERYK-------LNDLPDEEGRV-IEIDEWKR-------MIEKYLPTYAPIENV-----QNA 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 185 SIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHcngwSHTWAVTLS----GGTHVCLRKVQP 260
Cdd:PRK07638 145 PFYMGFTSGSTGKPKAFLRAQQSWLHSFDCNVHDFHMKREDSVLIAGTLVH----SLFLYGAIStlyvGQTVHLMRKFIP 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 261 DAIHAAIAEHAVTHLSAAPVVMSMLIHAEhaSVPAVPVSVITGGAAPPSAVIAAMEARgFSITHAYgmtESYGPSTLclw 340
Cdd:PRK07638 221 NQVLDKLETENISVMYTVPTMLESLYKEN--RVIENKMKIISSGAKWEAEAKEKIKNI-FPYAKLY---EFYGASEL--- 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 341 qpgvdelplearaQFMSrqgVAHPmlEEATVLDTDTGRPVP--------ADGRT-----LGELVVRGNTVMKGYLHNPEA 407
Cdd:PRK07638 292 -------------SFVT---ALVD--EESERRPNSVGRPFHnvqvricnEAGEEvqkgeIGTVYVKSPQFFMGYIIGGVL 353
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 408 TRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALR 487
Cdd:PRK07638 354 ARELNADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPVAIIKGS 433
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 489250734 488 ADAlprttgDDLVRWCRERLAHFKAPRHVTLT-ELPKTATGKIQKFVLREWARQEEE 543
Cdd:PRK07638 434 ATK------QQLKSFCLQRLSSFKIPKEWHFVdEIPYTNSGKIARMEAKSWIENQEK 484
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
34-529 |
3.68e-39 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 148.17 E-value: 3.68e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 34 PQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFIL 113
Cdd:cd17652 1 PDAPAVVFGDETLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYML 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 114 RHCAARVLIcdrefgavaqqalamldappllvgidddqAERADLAHdldyeaflaqgdpgrplsapqnewqsiaINYTSG 193
Cdd:cd17652 81 ADARPALLL-----------------------------TTPDNLAY----------------------------VIYTSG 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 194 TTGDPKGVVLHHRGayLNACAGALI--FQLGPRSIYL-WTLPMFHCNGWShTWAVTLSGGTHVCL---RKVQPDAIHAAI 267
Cdd:cd17652 104 STGRPKGVVVTHRG--LANLAAAQIaaFDVGPGSRVLqFASPSFDASVWE-LLMALLAGATLVLApaeELLPGEPLADLL 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 268 AEHAVTHLSAAPVVMSMLihaEHASVPAVPVSVITGGAAPPSavIAAMEARGFSITHAYGMTESYGPSTLCLWQPGVDEL 347
Cdd:cd17652 181 REHRITHVTLPPAALAAL---PPDDLPDLRTLVVAGEACPAE--LVDRWAPGRRMINAYGPTETTVCATMAGPLPGGGVP 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 348 PLearaqfmsrqGVAHPMlEEATVLDtDTGRPVPAdGRTlGELVVRGNTVMKGYLHNPEATR--------AALADGWLHT 419
Cdd:cd17652 256 PI----------GRPVPG-TRVYVLD-ARLRPVPP-GVP-GELYIAGAGLARGYLNRPGLTAerfvadpfGAPGSRMYRT 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 420 GDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVAlrADALPRTTGDDL 499
Cdd:cd17652 322 GDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEHPGVAEAVVVVRDDRPGDKRLVAYVV--PAPGAAPTAAEL 399
|
490 500 510
....*....|....*....|....*....|.
gi 489250734 500 VRWCRERLAHFKAPRH-VTLTELPKTATGKI 529
Cdd:cd17652 400 RAHLAERLPGYMVPAAfVVLDALPLTPNGKL 430
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
46-536 |
2.46e-38 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 145.79 E-value: 2.46e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 46 YSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSiafiLRHcaaRVlicDR 125
Cdd:cd05974 1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPATTLLTPDD----LRD---RV---DR 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 126 EFGAVAQQalamldappllvgiddDQAERADlahdldyeaflaqgDPgrplsapqnewqsIAINYTSGTTGDPKGVVLHH 205
Cdd:cd05974 71 GGAVYAAV----------------DENTHAD--------------DP-------------MLLYFTSGTTSKPKLVEHTH 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 206 RGAYLNACAGALIFQLGPRSIYL-WTLPMFHCNGWSHTWAVTLSGGTHVCLR--KVQPDAIHAAIAEHAVTHLSAAPVVM 282
Cdd:cd05974 108 RSYPVGHLSTMYWIGLKPGDVHWnISSPGWAKHAWSCFFAPWNAGATVFLFNyaRFDAKRVLAALVRYGVTTLCAPPTVW 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 283 SMLIHAEHASVpAVPVSVITGGAAP--PSAVIAAMEARGFSITHAYGMTES---YGPSTLCLWQPGVDELPLEA-RAQFM 356
Cdd:cd05974 188 RMLIQQDLASF-DVKLREVVGAGEPlnPEVIEQVRRAWGLTIRDGYGQTETtalVGNSPGQPVKAGSMGRPLPGyRVALL 266
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 357 SRQGvaHPMLEEATVLDTDTGRPVpadgrtlgelvvrgnTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDR 436
Cdd:cd05974 267 DPDG--APATEGEVALDLGDTRPV---------------GLMKGYAGDPDKTAHAMRGGYYRTGDIAMRDEDGYLTYVGR 329
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 437 AKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALP-RTTGDDLVRWCRERLAHFKAPRH 515
Cdd:cd05974 330 ADDVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDPVRLSVPKAFIVLRAGYEPsPETALEIFRFSRERLAPYKRIRR 409
|
490 500
....*....|....*....|.
gi 489250734 516 VTLTELPKTATGKIQKFVLRE 536
Cdd:cd05974 410 LEFAELPKTISGKIRRVELRR 430
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
24-546 |
7.72e-38 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 147.25 E-value: 7.72e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 24 SFLKRAAQVYPQRDAVVY-----GARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLV 98
Cdd:cd05968 65 QLLDKWLADTRTRPALRWegedgTSRTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVV 144
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 99 CINIRLEARSIAFILRHCAARVLIC-------DREFGAVAQQALAMLDAPPLLVGIDDDQAERADLAH---DLDYEAFLA 168
Cdd:cd05968 145 PIFSGFGKEAAATRLQDAEAKALITadgftrrGREVNLKEEADKACAQCPTVEKVVVVRHLGNDFTPAkgrDLSYDEEKE 224
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 169 QGDPGRPLSAPQNEWQSIainYTSGTTGDPKGVVLHHRGAYLNACAG-ALIFQLGPRSIYLWTLPMfhcnGWSH-TWAV- 245
Cdd:cd05968 225 TAGDGAERTESEDPLMII---YTSGTTGKPKGTVHVHAGFPLKAAQDmYFQFDLKPGDLLTWFTDL----GWMMgPWLIf 297
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 246 -TLSGGTHVCLRKVQPDAIHAAIAEHAV-----THLSAAPVVMSMLIHAEHASVPAVPVSVI-----TGGAAPPSAVIAA 314
Cdd:cd05968 298 gGLILGATMVLYDGAPDHPKADRLWRMVedheiTHLGLSPTLIRALKPRGDAPVNAHDLSSLrvlgsTGEPWNPEPWNWL 377
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 315 MEARGFS---ITHAYGMTESYGpSTLClwqpGVDELPLEAraqfMSRQGVAHPMleEATVLDtDTGRPVPAdgrTLGELV 391
Cdd:cd05968 378 FETVGKGrnpIINYSGGTEISG-GILG----NVLIKPIKP----SSFNGPVPGM--KADVLD-ESGKPARP---EVGELV 442
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 392 VR----GNTvmKGYLHNP----EATRAALADGWLHtGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEV 463
Cdd:cd05968 443 LLapwpGMT--RGFWRDEdrylETYWSRFDNVWVH-GDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPAV 519
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 464 VEAAVVARPDSRWGETPHAFVALRADALPRTT-GDDLVRWCRERLAHFKAPRHVTLT-ELPKTATGKIQKFVLREwARQE 541
Cdd:cd05968 520 LESAAIGVPHPVKGEAIVCFVVLKPGVTPTEAlAEELMERVADELGKPLSPERILFVkDLPKTRNAKVMRRVIRA-AYLG 598
|
....*
gi 489250734 542 EEAGD 546
Cdd:cd05968 599 KELGD 603
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
44-542 |
1.01e-37 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 147.08 E-value: 1.01e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 44 RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEmleAHYgvpgagAVLVCINIR---------LEARSIAFILR 114
Cdd:cd05967 81 RTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPE---AAI------AMLACARIGaihsvvfggFAAKELASRID 151
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 115 HCAARVLI---CDREFGAVAQ------QALAMLDAPP---LLVGIDDDQAERADLAHDLDYEAFLAQGDPGRPLSAPQNE 182
Cdd:cd05967 152 DAKPKLIVtasCGIEPGKVVPykplldKALELSGHKPhhvLVLNRPQVPADLTKPGRDLDWSELLAKAEPVDCVPVAATD 231
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 183 wqSIAINYTSGTTGDPKGVVlHHRGAYLNACAGAL--IFQLGPRSIYLWTLPMfhcnGW--SHTWAV---TLSGGTHVcL 255
Cdd:cd05967 232 --PLYILYTSGTTGKPKGVV-RDNGGHAVALNWSMrnIYGIKPGDVWWAASDV----GWvvGHSYIVygpLLHGATTV-L 303
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 256 RKVQPDAIH------AAIAEHAVTHLSAAPVVMSML-------IHAEHASVPAVPVSVITGGAAPPSAVIAAMEARGFSI 322
Cdd:cd05967 304 YEGKPVGTPdpgafwRVIEKYQVNALFTAPTAIRAIrkedpdgKYIKKYDLSSLRTLFLAGERLDPPTLEWAENTLGVPV 383
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 323 THAYGMTESYGP-STLCLwqpGVDELPLEARAQfmsrqGVAHPMLEeATVLDtDTGRPVPAdgRTLGELVVRG------- 394
Cdd:cd05967 384 IDHWWQTETGWPiTANPV---GLEPLPIKAGSP-----GKPVPGYQ-VQVLD-EDGEPVGP--NELGNIVIKLplppgcl 451
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 395 NTVMKgylhNPEATRA---ALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVAR 471
Cdd:cd05967 452 LTLWK----NDERFKKlylSKFPGYYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHPAVAECAVVGV 527
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 489250734 472 PDSRWGETPHAFVALRADA--LPRTTGDDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKIQKFVLREWARQEE 542
Cdd:cd05967 528 RDELKGQVPLGLVVLKEGVkiTAEELEKELVALVREQIGPVAAFRLVIFVKrLPKTRSGKILRRTLRKIADGED 601
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
21-529 |
1.10e-37 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 145.52 E-value: 1.10e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 21 SPLSFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCI 100
Cdd:PRK13383 36 NPYTLLAVTAARWPGRTAIIDDDGALSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPI 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 101 NIRLEARSIAFILRHCAARVLICDREFG---AVAQQALAMLDapPLLVGidddqaeradlahdldyeaflAQGDPGRPLS 177
Cdd:PRK13383 116 STEFRSDALAAALRAHHISTVVADNEFAeriAGADDAVAVID--PATAG---------------------AEESGGRPAV 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 178 APQNEwqsiAINYTSGTTGDPKGVvlhHRGAYLNACAGALI-------FQLGPRSIYlwTLPMFHCNGWSHTWAVTLSGG 250
Cdd:PRK13383 173 AAPGR----IVLLTSGTTGKPKGV---PRAPQLRSAVGVWVtildrtrLRTGSRISV--AMPMFHGLGLGMLMLTIALGG 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 251 THVCLRKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIH-----AEHASVPAVPVSVITGGAAPPSAVIAAMEARGFSITHA 325
Cdd:PRK13383 244 TVLTHRHFDAEAALAQASLHRADAFTAVPVVLARILElpprvRARNPLPQLRVVMSSGDRLDPTLGQRFMDTYGDILYNG 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 326 YGMTESygpstlclwqpGVDEL--PLEARAqfmSRQGVAHPMLEEATVLDTDTGRPVPAdgRTLGELVVRGNTVMKGYlh 403
Cdd:PRK13383 324 YGSTEV-----------GIGALatPADLRD---APETVGKPVAGCPVRILDRNNRPVGP--RVTGRIFVGGELAGTRY-- 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 404 nPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAF 483
Cdd:PRK13383 386 -TDGGGKAVVDGMTSTGDMGYLDNAGRLFIVGREDDMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERFGHRLAAF 464
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 489250734 484 VALRADAlpRTTGDDLVRWCRERLAHFKAPRHVTL-TELPKTATGKI 529
Cdd:PRK13383 465 VVLHPGS--GVDAAQLRDYLKDRVSRFEQPRDINIvSSIPRNPTGKV 509
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
34-529 |
2.02e-37 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 144.34 E-value: 2.02e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 34 PQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFIL 113
Cdd:cd12114 1 PDATAVICGDGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAARREAIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 114 RHCAARVlicdrefgAVAQQALAMLDAPPLLVGIDDDQAERAdlahdldyeaflaqgdPGRPLSAPQNEWQSIAINYTSG 193
Cdd:cd12114 81 ADAGARL--------VLTDGPDAQLDVAVFDVLILDLDALAA----------------PAPPPPVDVAPDDLAYVIFTSG 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 194 TTGDPKGVVLHHRGAyLNACAgALI--FQLGPRSIYLwTLPMFHCNgWS--HTWAVTLSGGTHV---CLRKVQPDAIHAA 266
Cdd:cd12114 137 STGTPKGVMISHRAA-LNTIL-DINrrFAVGPDDRVL-ALSSLSFD-LSvyDIFGALSAGATLVlpdEARRRDPAHWAEL 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 267 IAEHAVTHLSAAPVVMSMLIhAEHASVPAVPVS---VITGG-----AAPPSAVIAAMEARGFSIThayGMTESygpSTLC 338
Cdd:cd12114 213 IERHGVTLWNSVPALLEMLL-DVLEAAQALLPSlrlVLLSGdwiplDLPARLRALAPDARLISLG---GATEA---SIWS 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 339 LWQPgVDELPLEARA----QFMSRQgvahpmleEATVLDTDtGRPVPaDGrTLGELVVRGNTVMKGYLHNPEATRA---- 410
Cdd:cd12114 286 IYHP-IDEVPPDWRSipygRPLANQ--------RYRVLDPR-GRDCP-DW-VPGELWIGGRGVALGYLGDPELTAArfvt 353
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 411 -ALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARpDSRWGETPHAFVALRAD 489
Cdd:cd12114 354 hPDGERLYRTGDLGRYRPDGTLEFLGRRDGQVKVRGYRIELGEIEAALQAHPGVARAVVVVL-GDPGGKRLAAFVVPDND 432
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 489250734 490 ALPRTTgDDLVRWCRERLAHFKAPRHV-TLTELPKTATGKI 529
Cdd:cd12114 433 GTPIAP-DALRAFLAQTLPAYMIPSRViALEALPLTANGKV 472
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
28-547 |
2.58e-37 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 147.70 E-value: 2.58e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 28 RAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEAR 107
Cdd:COG1020 484 AQAARTPDAVAVVFGDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVPLDPAYPAE 563
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 108 SIAFILRHCAARVLICDREFgavaQQALAMLDAPPLLVgiddDQAERADLAHDldyeaflaqgDPGRPLSApqnewQSIA 187
Cdd:COG1020 564 RLAYMLEDAGARLVLTQSAL----AARLPELGVPVLAL----DALALAAEPAT----------NPPVPVTP-----DDLA 620
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 188 -INYTSGTTGDPKGVVLHHRGA--YLNACAGAliFQLGPRSIYLWTLPM-FHCNGWSHTWAVtLSGGTHVCLRK---VQP 260
Cdd:COG1020 621 yVIYTSGSTGRPKGVMVEHRALvnLLAWMQRR--YGLGPGDRVLQFASLsFDASVWEIFGAL-LSGATLVLAPPearRDP 697
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 261 DAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVsVITGGAAPPSAVIAAMEAR--GFSITHAYGMTESygpSTLC 338
Cdd:COG1020 698 AALAELLARHRVTVLNLTPSLLRALLDAAPEALPSLRL-VLVGGEALPPELVRRWRARlpGARLVNLYGPTET---TVDS 773
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 339 LWQPgVDELPLEARaqfmsRQGVAHPMLE-EATVLDtDTGRPVPaDGRTlGELVVRGNTVMKGYLHNPEATRAA-LADGW 416
Cdd:COG1020 774 TYYE-VTPPDADGG-----SVPIGRPIANtRVYVLD-AHLQPVP-VGVP-GELYIGGAGLARGYLNRPELTAERfVADPF 844
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 417 LH-------TGDLAVLHPDGYVEIKDRAKD-IIISG-----GenisslEIEEVLYQHPEVVEAAVVARPDsrwGETPHAF 483
Cdd:COG1020 845 GFpgarlyrTGDLARWLPDGNLEFLGRADDqVKIRGfrielG------EIEAALLQHPGVREAVVVARED---APGDKRL 915
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 489250734 484 VALRADALPRTTGDDLVRWCRERLAHFKAPRHVTLTELPKTATGKIQKFVLREWARQEEEAGDA 547
Cdd:COG1020 916 VAYVVPEAGAAAAAALLRLALALLLPPYMVPAAVVLLLPLPLTGNGKLDRLALPAPAAAAAAAA 979
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
34-535 |
1.15e-36 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 141.74 E-value: 1.15e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 34 PQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFIL 113
Cdd:cd17649 1 PDAVALVFGDQSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAERLRYML 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 114 RHCAARVLicdrefgavaqqalamldappllvgidddqaeradLAHDLDYEAFLAqgdpgrplsapqnewqsiainYTSG 193
Cdd:cd17649 81 EDSGAGLL-----------------------------------LTHHPRQLAYVI---------------------YTSG 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 194 TTGDPKGVVLHHrGAYLNAC-AGALIFQLGPRSIYLWTLPmFHCNGWSHTWAVTLSGGTHVCLRKVQ----PDAIHAAIA 268
Cdd:cd17649 105 STGTPKGVAVSH-GPLAAHCqATAERYGLTPGDRELQFAS-FNFDGAHEQLLPPLICGACVVLRPDElwasADELAEMVR 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 269 EHAVTHLSAAPVVMSMLIHAEHASVPAVPVSV---ITGGAAPPSAVIAAMEARGFSITHAYGMTESYGPSTLClwqpgvd 345
Cdd:cd17649 183 ELGVTVLDLPPAYLQQLAEEADRTGDGRPPSLrlyIFGGEALSPELLRRWLKAPVRLFNAYGPTEATVTPLVW------- 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 346 elplEARAQFmSRQGVAHPM-----LEEATVLDTDtGRPVPaDGRTlGELVVRGNTVMKGYLHNPEATRAAL------AD 414
Cdd:cd17649 256 ----KCEAGA-ARAGASMPIgrplgGRSAYILDAD-LNPVP-VGVT-GELYIGGEGLARGYLGRPELTAERFvpdpfgAP 327
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 415 G--WLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRwGETPHAFVALRADALP 492
Cdd:cd17649 328 GsrLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVVALDGAG-GKQLVAYVVLRAAAAQ 406
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 489250734 493 RTTGDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQKFVLR 535
Cdd:cd17649 407 PELRAQLRTALRASLPDYMVPAHlVFLARLPLTPNGKLDRKALP 450
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
59-539 |
1.32e-36 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 143.09 E-value: 1.32e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 59 ASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDREFGAVAQQALAml 138
Cdd:PRK08751 65 AYLLGELQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVVIDNFGTTVQQVIA-- 142
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 139 DAP---PLLVGIDD--DQAERADLAHDLDY-----------------EAfLAQGDPGRpLSAPQNEWQSIA-INYTSGTT 195
Cdd:PRK08751 143 DTPvkqVITTGLGDmlGFPKAALVNFVVKYvkklvpeyringairfrEA-LALGRKHS-MPTLQIEPDDIAfLQYTGGTT 220
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 196 GDPKGVVLHHRGAYLNAC-AGALIFQLGP----RSIYLWTLPMFHCNGWSHTWAV--TLSGGTHVCLRKVQPDAIHAAIA 268
Cdd:PRK08751 221 GVAKGAMLTHRNLVANMQqAHQWLAGTGKleegCEVVITALPLYHIFALTANGLVfmKIGGCNHLISNPRDMPGFVKELK 300
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 269 EHAVTHLSAAPVVMSMLIHA---EHASVPAVPVSVITGGAAPPSAVIAAMEARGFSITHAYGMTESygpstlclwQPGVD 345
Cdd:PRK08751 301 KTRFTAFTGVNTLFNGLLNTpgfDQIDFSSLKMTLGGGMAVQRSVAERWKQVTGLTLVEAYGLTET---------SPAAC 371
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 346 ELPLEARAQFMSrqgVAHPMLEEATVLDTDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGDLAV 424
Cdd:PRK08751 372 INPLTLKEYNGS---IGLPIPSTDACIKDDAGTVLAIG--EIGELCIKGPQVMKGYWKRPEETAKVMdADGWLHTGDIAR 446
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 425 LHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAfVALRADalPRTTGDDLVRWCR 504
Cdd:PRK08751 447 MDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVIAMMPGVLEVAAVGVPDEKSGEIVKV-VIVKKD--PALTAEDVKAHAR 523
|
490 500 510
....*....|....*....|....*....|....*.
gi 489250734 505 ERLAHFKAPRHVTL-TELPKTATGKIQKFVLREWAR 539
Cdd:PRK08751 524 ANLTGYKQPRIIEFrKELPKTNVGKILRRELRDAAK 559
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
18-536 |
1.76e-35 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 139.72 E-value: 1.76e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 18 VPLSPLSFLKRAAQVYPQRDAVVYGA----RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGA 93
Cdd:cd05906 8 APRTLLELLLRAAERGPTKGITYIDAdgseEFQSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWACVLA 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 94 GAV----LVCINIRLEARSIAFiLRHcaarvlicdrefgavaqqALAMLDAPPLLVGiDDDQAERADLAHDLDYEAFL-- 167
Cdd:cd05906 88 GFVpaplTVPPTYDEPNARLRK-LRH------------------IWQLLGSPVVLTD-AELVAEFAGLETLSGLPGIRvl 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 168 ---AQGDPGRPLSAPQNEWQSIA-INYTSGTTGDPKGVVLHHRgAYLNACAGALI-FQLGPRSIYLWTLPMFHCNG--WS 240
Cdd:cd05906 148 sieELLDTAADHDLPQSRPDDLAlLMLTSGSTGFPKAVPLTHR-NILARSAGKIQhNGLTPQDVFLNWVPLDHVGGlvEL 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 241 HTWAVTLsGGTHVclrKVQPDaihaaiaehavtHLSAAPVVMSMLIHAEHASVPAVP--------------------VS- 299
Cdd:cd05906 227 HLRAVYL-GCQQV---HVPTE------------EILADPLRWLDLIDRYRVTITWAPnfafallndlleeiedgtwdLSs 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 300 ---VITGGAAPPSAVIAAM-------EARGFSITHAYGMTESygpSTLCLWQPGVDELPLEARAQFMSrqgVAHPMLE-E 368
Cdd:cd05906 291 lryLVNAGEAVVAKTIRRLlrllepyGLPPDAIRPAFGMTET---CSGVIYSRSFPTYDHSQALEFVS---LGRPIPGvS 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 369 ATVLDtDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGDLAVLHpDGYVEIKDRAKDIIISGGEN 447
Cdd:cd05906 365 MRIVD-DEGQLLPEG--EVGRLQVRGPVVTKGYYNNPEANAEAFtEDGWFRTGDLGFLD-NGNLTITGRTKDTIIVNGVN 440
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 448 ISSLEIEEVLYQHPEVVE---AAVVARPDSRwgETPHAFV-----ALRADALPRTtgddlVRWCRERLAHFK--APRHV- 516
Cdd:cd05906 441 YYSHEIEAAVEEVPGVEPsftAAFAVRDPGA--ETEELAIffvpeYDLQDALSET-----LRAIRSVVSREVgvSPAYLi 513
|
570 580
....*....|....*....|..
gi 489250734 517 --TLTELPKTATGKIQKFVLRE 536
Cdd:cd05906 514 plPKEEIPKTSLGKIQRSKLKA 535
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
35-536 |
1.10e-34 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 137.47 E-value: 1.10e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 35 QRDAVVYGARRYSYRQLHERSCALASALERVGVQAGE-RVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFIL 113
Cdd:PRK13388 16 DTIAVRYGDRTWTWREVLAEAAARAAALIALADPDRPlHVGVLLGNTPEMLFWLAAAALGGYVLVGLNTTRRGAALAADI 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 114 RHCAARVLICDREFGAVaqqaLAMLDAP--PLLVGIDDDQAERADLAHDLdyeaflaqgdpgRPLSAPQNEWQSIAInYT 191
Cdd:PRK13388 96 RRADCQLLVTDAEHRPL----LDGLDLPgvRVLDVDTPAYAELVAAAGAL------------TPHREVDAMDPFMLI-FT 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 192 SGTTGDPKGVVLHHrgAYLNACAGALI--FQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLR-KVQPDAIHAAIA 268
Cdd:PRK13388 159 SGTTGAPKAVRCSH--GRLAFAGRALTerFGLTRDDVCYVSMPLFHSNAVMAGWAPAVASGAAVALPaKFSASGFLDDVR 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 269 EHAVTHLSAAPVVMS-MLIHAEHASVPAVPVSVITGGAAPPSAvIAAMEAR-GFSITHAYGMTESYGPSTLclwQPGVDE 346
Cdd:PRK13388 237 RYGATYFNYVGKPLAyILATPERPDDADNPLRVAFGNEASPRD-IAEFSRRfGCQVEDGYGSSEGAVIVVR---EPGTPP 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 347 LPLEARAQfmsrqGVAhpmleeatVLDTDTGRPVP-----ADGRTL------GELVVR-GNTVMKGYLHNPEATRAALAD 414
Cdd:PRK13388 313 GSIGRGAP-----GVA--------IYNPETLTECAvarfdAHGALLnadeaiGELVNTaGAGFFEGYYNNPEATAERMRH 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 415 GWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADAlpRT 494
Cdd:PRK13388 380 GMYWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAAPIERILLRHPAINRVAVYAVPDERVGDQVMAALVLRDGA--TF 457
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 489250734 495 TGDDLVRWCRER--LAHFKAPRHVTL-TELPKTATGKIQKFVLRE 536
Cdd:PRK13388 458 DPDAFAAFLAAQpdLGTKAWPRYVRIaADLPSTATNKVLKRELIA 502
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
46-493 |
1.19e-34 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 136.83 E-value: 1.19e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 46 YSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDR 125
Cdd:cd05932 7 FTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKALFVGK 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 126 -----EFGAVAQQALAMLDAPPLLVGIDDDQaeradlahdldYEAFLAQGDP--GRPLSAPQnewQSIAINYTSGTTGDP 198
Cdd:cd05932 87 lddwkAMAPGVPEGLISISLPPPSAANCQYQ-----------WDDLIAQHPPleERPTRFPE---QLATLIYTSGTTGQP 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 199 KGVvLHHRGAYLNACAGAL-IFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVC-----------LRKVQP------ 260
Cdd:cd05932 153 KGV-MLTFGSFAWAAQAGIeHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVLVAfaesldtfvedVQRARPtlffsv 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 261 --------DAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVSVITGGAAP-PSAVIAAMEARGFSITHAYGMTES 331
Cdd:cd05932 232 prlwtkfqQGVQDKIPQQKLNLLLKIPVVNSLVKRKVLKGLGLDQCRLAGCGSAPvPPALLEWYRSLGLNILEAYGMTEN 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 332 YGPSTLClwQPGVDelplearaqfmsRQGVAHPMLEEATVLDTDTgrpvpadgrtlGELVVRGNTVMKGYLHNPEATRAA 411
Cdd:cd05932 312 FAYSHLN--YPGRD------------KIGTVGNAGPGVEVRISED-----------GEILVRSPALMMGYYKDPEATAEA 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 412 L-ADGWLHTGDLAVLHPDGYVEIKDRAKDII-ISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRwgetPHAFVALRAD 489
Cdd:cd05932 367 FtADGFLRTGDKGELDADGNLTITGRVKDIFkTSKGKYVAPAPIENKLAEHDRVEMVCVIGSGLPA----PLALVVLSEE 442
|
....
gi 489250734 490 ALPR 493
Cdd:cd05932 443 ARLR 446
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
25-529 |
1.34e-34 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 135.52 E-value: 1.34e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 25 FLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRL 104
Cdd:cd12115 4 LVEAQAARTPDAIALVCGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLDPAY 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 105 EARSIAFILRHCAARVLICDrefgavaqqalamldappllvgidddqaeradlAHDLDYeaflaqgdpgrplsapqnewq 184
Cdd:cd12115 84 PPERLRFILEDAQARLVLTD---------------------------------PDDLAY--------------------- 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 185 siaINYTSGTTGDPKGVVLHHRGaylnacAGALIFqlgprsiylWTLPMFHCNGWSHTWAVT-----LS----------G 249
Cdd:cd12115 110 ---VIYTSGSTGRPKGVAIEHRN------AAAFLQ---------WAAAAFSAEELAGVLASTsicfdLSvfelfgplatG 171
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 250 GTHVCLRKVQpdAIHAAIAEHAVTHLSAAPVVMSMLIhaEHASVPAVPVSVITGGAAPPSAVIAAMEAR--GFSITHAYG 327
Cdd:cd12115 172 GKVVLADNVL--ALPDLPAAAEVTLINTVPSAAAELL--RHDALPASVRVVNLAGEPLPRDLVQRLYARlqVERVVNLYG 247
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 328 MTESYGPSTLCLWQPGVDELPLEARAQFMSRqgvahpmleeATVLDtDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEA 407
Cdd:cd12115 248 PSEDTTYSTVAPVPPGASGEVSIGRPLANTQ----------AYVLD-RALQPVPLG--VPGELYIGGAGVARGYLGRPGL 314
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 408 TRAA-LADGWL------HTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETP 480
Cdd:cd12115 315 TAERfLPDPFGpgarlyRTGDLVRWRPDGLLEFLGRADNQVKVRGFRIELGEIEAALRSIPGVREAVVVAIGDAAGERRL 394
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 489250734 481 HAFVALRADALPRTTgdDLVRWCRERLAHFKAP-RHVTLTELPKTATGKI 529
Cdd:cd12115 395 VAYIVAEPGAAGLVE--DLRRHLGTRLPAYMVPsRFVRLDALPLTPNGKI 442
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
45-537 |
3.22e-34 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 136.06 E-value: 3.22e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 45 RYSYRQLHERSCALASALERV-GVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLIC 123
Cdd:cd05928 41 KWSFRELGSLSRKAANVLSGAcGLQRGDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQLTAKDILYRLQASKAKCIVT 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 124 DREFgAVAQQALAmLDAP----PLLVgidddqaerADLAHD--LDYEAFLAQGDPGRPLSAPQNEwQSIAINYTSGTTGD 197
Cdd:cd05928 121 SDEL-APEVDSVA-SECPslktKLLV---------SEKSRDgwLNFKELLNEASTEHHCVETGSQ-EPMAIYFTSGTTGS 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 198 PKgVVLHHRGAY---LNACAGALIfQLGPRSIyLWTLPMfhcNGW--SHTWAVTLSGGTHVC-----LRKVQPDAIHAAI 267
Cdd:cd05928 189 PK-MAEHSHSSLglgLKVNGRYWL-DLTASDI-MWNTSD---TGWikSAWSSLFEPWIQGACvfvhhLPRFDPLVILKTL 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 268 AEHAVTHLSAAPVVMSMLIHAEHASV--PAVPVSVITGGAAPPSAVIAAMEARGFSITHAYGMTESygpSTLCLWQPGvd 345
Cdd:cd05928 263 SSYPITTFCGAPTVYRMLVQQDLSSYkfPSLQHCVTGGEPLNPEVLEKWKAQTGLDIYEGYGQTET---GLICANFKG-- 337
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 346 elpLEARAQFMSRQGVAHpmleEATVLDtDTGRPVPADgrTLGELVVRGNTV-----MKGYLHNPEATRAALADGWLHTG 420
Cdd:cd05928 338 ---MKIKPGSMGKASPPY----DVQIID-DNGNVLPPG--TEGDIGIRVKPIrpfglFSGYVDNPEKTAATIRGDFYLTG 407
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 421 DLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPRTTgDDLV 500
Cdd:cd05928 408 DRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGEVVKAFVVLAPQFLSHDP-EQLT 486
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 489250734 501 RWCRERL----AHFKAPRHVT-LTELPKTATGKIQKFVLR--EW 537
Cdd:cd05928 487 KELQQHVksvtAPYKYPRKVEfVQELPKTVTGKIQRNELRdkEW 530
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
16-534 |
3.37e-34 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 135.91 E-value: 3.37e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 16 NHVPLSPLSFLKRAAQVYPQRDAVVY--GARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGA 93
Cdd:PRK05857 10 PQLPSTVLDRVFEQARQQPEAIALRRcdGTSALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACAKL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 94 GAVLVCINIRLEARSIAFILRHCAARVLICDREFGAVAQQALAMLDAPPLLVGIDDDQAERADLAHDLDYEAflaqGDPG 173
Cdd:PRK05857 90 GAIAVMADGNLPIAAIERFCQITDPAAALVAPGSKMASSAVPEALHSIPVIAVDIAAVTRESEHSLDAASLA----GNAD 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 174 RPLSAPqnewqsIAINYTSGTTGDPKGVVLHHRGAY-----LNACAGALIFQLGPRSIYLwTLPMFHCNG-WshtWAVT- 246
Cdd:PRK05857 166 QGSEDP------LAMIFTSGTTGEPKAVLLANRTFFavpdiLQKEGLNWVTWVVGETTYS-PLPATHIGGlW---WILTc 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 247 -------LSGGTH-VCLRKVQPDAIhaaiaehaVTHLSAAPVVMSMLIHAEHASVPAVPV--SVITGGAAPPSAVIAAME 316
Cdd:PRK05857 236 lmhgglcVTGGENtTSLLEILTTNA--------VATTCLVPTLLSKLVSELKSANATVPSlrLVGYGGSRAIAADVRFIE 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 317 ARGFSITHAYGMTESyGPSTLCLWQPGVDELPLEARAQFMSRQGVahpmleEATVLDTDTGRPVPADG---RTLGELVVR 393
Cdd:PRK05857 308 ATGVRTAQVYGLSET-GCTALCLPTDDGSIVKIEAGAVGRPYPGV------DVYLAATDGIGPTAPGAgpsASFGTLWIK 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 394 GNTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPD 473
Cdd:PRK05857 381 SPANMLGYWNNPERTAEVLIDGWVNTGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEVDRIAEGVSGVREAACYEIPD 460
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 489250734 474 SRWGetphAFVALR-----------ADALPRTTGddlVRWCRERLAHFKAPRHVTLTELPKTATGKIQKFVL 534
Cdd:PRK05857 461 EEFG----ALVGLAvvasaeldesaARALKHTIA---ARFRRESEPMARPSTIVIVTDIPRTQSGKVMRASL 525
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
44-546 |
1.01e-33 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 135.38 E-value: 1.01e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 44 RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMleahygvpgAGAVLVCINI---------RLEARSIAFILR 114
Cdd:cd05966 83 RTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPEL---------VIAMLACARIgavhsvvfaGFSAESLADRIN 153
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 115 HCAARVLICDREF---------GAVAQQALAMldAPPL---LV----GID-DDQAERadlahDLDYEAFLAQGDPGRPLs 177
Cdd:cd05966 154 DAQCKLVITADGGyrggkviplKEIVDEALEK--CPSVekvLVvkrtGGEvPMTEGR-----DLWWHDLMAKQSPECEP- 225
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 178 apqnEWQS----IAINYTSGTTGDPKGVVlHHRGAYLNACAGAL--IFQLGPRSIYlWtlpmfhCN---GW--SHTWAV- 245
Cdd:cd05966 226 ----EWMDsedpLFILYTSGSTGKPKGVV-HTTGGYLLYAATTFkyVFDYHPDDIY-W------CTadiGWitGHSYIVy 293
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 246 --TLSGGTHVCLRKV----QPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVS---VITGGAAP--PSA---- 310
Cdd:cd05966 294 gpLANGATTVMFEGTptypDPGRYWDIVEKHKVTIFYTAPTAIRALMKFGDEWVKKHDLSslrVLGSVGEPinPEAwmwy 373
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 311 --VIAAMEArgfSITHAYGMTESYGPSTLCLwqPGVDEL-PLEARAQFMsrqGVahpmleEATVLDTDtGRPVPADGRtl 387
Cdd:cd05966 374 yeVIGKERC---PIVDTWWQTETGGIMITPL--PGATPLkPGSATRPFF---GI------EPAILDEE-GNEVEGEVE-- 436
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 388 GELVVRG------NTV-------MKGYLHN-PeatraaladGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEI 453
Cdd:cd05966 437 GYLVIKRpwpgmaRTIygdheryEDTYFSKfP---------GYYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEV 507
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 454 EEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALP-RTTGDDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKIQK 531
Cdd:cd05966 508 ESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDGEEPsDELRKELRKHVRKEIGPIATPDKIQFVPgLPKTRSGKIMR 587
|
570
....*....|....*
gi 489250734 532 FVLREWARQEEEAGD 546
Cdd:cd05966 588 RILRKIAAGEEELGD 602
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
27-536 |
1.62e-33 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 132.43 E-value: 1.62e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 27 KRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEA 106
Cdd:cd17653 4 ERIAAAHPDAVAVESLGGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAKLPS 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 107 RSIAFILRHCAARVLICdrefgavaqqalamldappllvgidddqaerADLAHDLDYeaflaqgdpgrplsapqnewqsi 186
Cdd:cd17653 84 ARIQAILRTSGATLLLT-------------------------------TDSPDDLAY----------------------- 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 187 aINYTSGTTGDPKGVVLHHRG--AYLNACAGALIFQLGPRSIYLWTlPMFHCNGWShtWAVTLSGGTHVCLRKvqpDAIH 264
Cdd:cd17653 110 -IIFTSGSTGIPKGVMVPHRGvlNYVSQPPARLDVGPGSRVAQVLS-IAFDACIGE--IFSTLCNGGTLVLAD---PSDP 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 265 AAIAEHAVTHLSAAPVVMSMLIHAEHASVPavpvSVITGGAAPPSAVIAAMeARGFSITHAYGMTESYGPSTLCLWQPGV 344
Cdd:cd17653 183 FAHVARTVDALMSTPSILSTLSPQDFPNLK----TIFLGGEAVPPSLLDRW-SPGRRLYNAYGPTECTISSTMTELLPGQ 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 345 delPLEARAQfMSRQGVahpmleeaTVLDTDTgRPVPADgrTLGELVVRGNTVMKGYLHNPEAT-RAALADGWLH----- 418
Cdd:cd17653 258 ---PVTIGKP-IPNSTC--------YILDADL-QPVPEG--VVGEICISGVQVARGYLGNPALTaSKFVPDPFWPgsrmy 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 419 -TGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVE--AAVVARpdsrwgETPHAFVAlradalPRTT 495
Cdd:cd17653 323 rTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLEEIEEVVLQSQPEVTqaAAIVVN------GRLVAFVT------PETV 390
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 489250734 496 GDDLVR-WCRERLAHFKAP-RHVTLTELPKTATGKIQKFVLRE 536
Cdd:cd17653 391 DVDGLRsELAKHLPSYAVPdRIIALDSFPLTANGKVDRKALRE 433
|
|
| Ac_CoA_lig_AcsA |
TIGR02188 |
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ... |
44-545 |
3.84e-33 |
|
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.
Pssm-ID: 274022 [Multi-domain] Cd Length: 626 Bit Score: 133.91 E-value: 3.84e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 44 RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLIC 123
Cdd:TIGR02188 87 RKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIAMLACARIGAIHSVVFGGFSAEALADRINDAGAKLVIT 166
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 124 DREF---------GAVAQQALAMldaPPLLV---------GIDDD--QAERadlahDLDYEaFLAQGDPGRPLSAPQNEW 183
Cdd:TIGR02188 167 ADEGlrggkviplKAIVDEALEK---CPVSVehvlvvrrtGNPVVpwVEGR-----DVWWH-DLMAKASAYCEPEPMDSE 237
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 184 QSIAINYTSGTTGDPKGVVlHHRGAYLNACA--GALIFQLGPRSIYlWtlpmfhCN---GW--SHTWAV---TLSGGTHV 253
Cdd:TIGR02188 238 DPLFILYTSGSTGKPKGVL-HTTGGYLLYAAmtMKYVFDIKDGDIF-W------CTadvGWitGHSYIVygpLANGATTV 309
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 254 CLRKV----QPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHA--------------SV--PAVP------VSVITGGAAP 307
Cdd:TIGR02188 310 MFEGVptypDPGRFWEIIEKHKVTIFYTAPTAIRALMRLGDEwvkkhdlsslrllgSVgePINPeawmwyYKVVGKERCP 389
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 308 PSAVIAAMEARGFSITHAYGMTESygpstlclwQPGVDELPLEaraqfmsrqGVahpmleEATVLDtDTGRPVPADGRTl 387
Cdd:TIGR02188 390 IVDTWWQTETGGIMITPLPGATPT---------KPGSATLPFF---------GI------EPAVVD-EEGNPVEGPGEG- 443
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 388 GELVVRGN--TVMKGYLHNPE---ATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPE 462
Cdd:TIGR02188 444 GYLVIKQPwpGMLRTIYGDHErfvDTYFSPFPGYYFTGDGARRDKDGYIWITGRVDDVINVSGHRLGTAEIESALVSHPA 523
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 463 VVEAAVVARPDSRWGETPHAFVALRADalpRTTGDDLVR----WCRERLAHFKAPRHVTLTE-LPKTATGKIQKFVLREW 537
Cdd:TIGR02188 524 VAEAAVVGIPDDIKGQAIYAFVTLKDG---YEPDDELRKelrkHVRKEIGPIAKPDKIRFVPgLPKTRSGKIMRRLLRKI 600
|
....*...
gi 489250734 538 ARQEEEAG 545
Cdd:TIGR02188 601 AAGEAEIL 608
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
187-531 |
2.97e-32 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 126.99 E-value: 2.97e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 187 AINYTSGTTGDPKGVVLHHRGAYLnACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTL---SGGTHVCLRKVQPDAI 263
Cdd:cd17635 5 AVIFTSGTTGEPKAVLLANKTFFA-VPDILQKEGLNWVVGDVTYLPLPATHIGGLWWILTClihGGLCVTGGENTTYKSL 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 264 HAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPV--SVITGGAAPPSAVIAAMEARGFS-ITHAYGMTESygpSTLCLW 340
Cdd:cd17635 84 FKILTTNAVTTTCLVPTLLSKLVSELKSANATVPSlrLIGYGGSRAIAADVRFIEATGLTnTAQVYGLSET---GTALCL 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 341 QPGVDELPLEAraqfmsrqgVAHPML-EEATVLDTDtGRPVPADGRtlGELVVRGNTVMKGYLHNPEATRAALADGWLHT 419
Cdd:cd17635 161 PTDDDSIEINA---------VGRPYPgVDVYLAATD-GIAGPSASF--GTIWIKSPANMLGYWNNPERTAEVLIDGWVNT 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 420 GDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPRTTGDDL 499
Cdd:cd17635 229 GDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVASAELDENAIRALK 308
|
330 340 350
....*....|....*....|....*....|..
gi 489250734 500 VRWCRERLAHFKAPRHVTLTELPKTATGKIQK 531
Cdd:cd17635 309 HTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
19-536 |
2.98e-32 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 130.76 E-value: 2.98e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 19 PLSPLSF---LKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGA 95
Cdd:PRK08279 33 PDSKRSLgdvFEEAAARHPDRPALLFEDQSISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGA 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 96 VLVCINIRLEARSIAfilrHC----AARVLICDREFGAVAQQALAMLDAPPLLVGIDDDQAERADLAHDLDyeAFLAQGD 171
Cdd:PRK08279 113 VVALLNTQQRGAVLA----HSlnlvDAKHLIVGEELVEAFEEARADLARPPRLWVAGGDTLDDPEGYEDLA--AAAAGAP 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 172 PGRPLSApqnewQSIAIN------YTSGTTGDPKGVVL-HHRgaYLNACAG-ALIFQLGPRSIYLWTLPMFHCNGWSHTW 243
Cdd:PRK08279 187 TTNPASR-----SGVTAKdtafyiYTSGTTGLPKAAVMsHMR--WLKAMGGfGGLLRLTPDDVLYCCLPLYHNTGGTVAW 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 244 AVTLSGGTHVCLRKvqpdaihaaiaehavtHLSA-----------APVVMS-------MLIHAEHASVPAVPVSVITGGA 305
Cdd:PRK08279 260 SSVLAAGATLALRR----------------KFSAsrfwddvrryrATAFQYigelcryLLNQPPKPTDRDHRLRLMIGNG 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 306 APPSaVIAAMEARgFSITH---AYGMTEsyGPSTLC--LWQPG-VDELPLEARAQFmsrqgvahpmleeATV-LDTDTGR 378
Cdd:PRK08279 324 LRPD-IWDEFQQR-FGIPRileFYAASE--GNVGFInvFNFDGtVGRVPLWLAHPY-------------AIVkYDVDTGE 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 379 PV-PADGRTL-------GELV--VRGNTVMKGYLhNPEATRAAL-------ADGWLHTGDLAVLHPDGYVEIKDRAKDII 441
Cdd:PRK08279 387 PVrDADGRCIkvkpgevGLLIgrITDRGPFDGYT-DPEASEKKIlrdvfkkGDAWFNTGDLMRDDGFGHAQFVDRLGDTF 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 442 ISGGENISSLEIEEVLYQHPEVVEAAV--VARP--DSRWGetphaFVALRADALPRTTGDDLVRWCRERLAHFKAPRHVT 517
Cdd:PRK08279 466 RWKGENVATTEVENALSGFPGVEEAVVygVEVPgtDGRAG-----MAAIVLADGAEFDLAALAAHLYERLPAYAVPLFVR 540
|
570 580
....*....|....*....|
gi 489250734 518 LT-ELPKTATGKIQKFVLRE 536
Cdd:PRK08279 541 LVpELETTGTFKYRKVDLRK 560
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
19-549 |
9.09e-32 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 131.23 E-value: 9.09e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 19 PLSPLSFLKRAAQV--YPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAV 96
Cdd:PRK12316 2000 PRGPGVHQRIAEQAarAPEAIAVVFGDQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGA 2079
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 97 LVCINIRLEARSIAFILRHCAARVLICDREfgavaqqalaMLDAPPLLVGIdddqaERADLAHDLDYEAFlAQGDPgRPL 176
Cdd:PRK12316 2080 YVPLDPNYPAERLAYMLEDSGAALLLTQRH----------LLERLPLPAGV-----ARLPLDRDAEWADY-PDTAP-AVQ 2142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 177 SAPQNEWQSIainYTSGTTGDPKGVVLHHrGAYLNAC-AGALIFQLGPRSIYLWTLPmFHCNGWSHTWAVTLSGGTHVCL 255
Cdd:PRK12316 2143 LAGENLAYVI---YTSGSTGLPKGVAVSH-GALVAHCqAAGERYELSPADCELQFMS-FSFDGAHEQWFHPLLNGARVLI 2217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 256 RKVQ---PDAIHAAIAEHAVTHLSAAPVVMSMLI-HAEHA-SVPAVPVSVITGGAAPPSAVIAAMEA-RGFSITHAYGMT 329
Cdd:PRK12316 2218 RDDElwdPEQLYDEMERHGVTILDFPPVYLQQLAeHAERDgRPPAVRVYCFGGEAVPAASLRLAWEAlRPVYLFNGYGPT 2297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 330 ESYgpSTLCLWQPGVDELPLEARAQFMSRQGVahpmlEEATVLDTDTgRPVPADGrtLGELVVRGNTVMKGYLHNPEATR 409
Cdd:PRK12316 2298 EAV--VTPLLWKCRPQDPCGAAYVPIGRALGN-----RRAYILDADL-NLLAPGM--AGELYLGGEGLARGYLNRPGLTA 2367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 410 AA-LADGWLH-------TGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARpDSRWGETPH 481
Cdd:PRK12316 2368 ERfVPDPFSAsgerlyrTGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPAVREAVVVAQ-DGASGKQLV 2446
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 482 AFVAlrADALPRTTGDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQKFVL-REWARQEEEAGDAGR 549
Cdd:PRK12316 2447 AYVV--PDDAAEDLLAELRAWLAARLPAYMVPAHwVVLERLPLNPNGKLDRKALpKPDVSQLRQAYVAPQ 2514
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
12-534 |
7.07e-31 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 128.74 E-value: 7.07e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 12 PNEANHVPLSPLSFLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVP 91
Cdd:PRK12467 504 APATEYAPDCVHQLIEAQARQHPERPALVFGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVL 583
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 92 GAGAVLVCINIRLEARSIAFILRHCAARVLICDREfgavaqqALAMLDAPPLLVGIDDDQAERadlahdldyeafLAQGD 171
Cdd:PRK12467 584 KAGGAYVPLDPEYPQDRLAYMLDDSGVRLLLTQSH-------LLAQLPVPAGLRSLCLDEPAD------------LLCGY 644
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 172 PGRPLSAPQNEWQSIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPR-SIYLWTLPMFHCNGWSHTWAVTLSGG 250
Cdd:PRK12467 645 SGHNPEVALDPDNLAYVIYTSGSTGQPKGVAISHGALANYVCVIAERLQLAADdSMLMVSTFAFDLGVTELFGALASGAT 724
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 251 THVCLRKV--QPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVSVITGGAAPPSAVIAAMEAR--GFSITHAY 326
Cdd:PRK12467 725 LHLLPPDCarDAEAFAALMADQGVTVLKIVPSHLQALLQASRVALPRPQRALVCGGEALQVDLLARVRALgpGARLINHY 804
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 327 GMTESygpstlclwQPGVDELPLEARAQFMSRQGVAHPMLEEAT-VLDTDTgRPVPadGRTLGELVVRGNTVMKGYLHNP 405
Cdd:PRK12467 805 GPTET---------TVGVSTYELSDEERDFGNVPIGQPLANLGLyILDHYL-NPVP--VGVVGELYIGGAGLARGYHRRP 872
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 406 --EATR-----AALADGWLH-TGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPdsrwG 477
Cdd:PRK12467 873 alTAERfvpdpFGADGGRLYrTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVLAQP----G 948
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 489250734 478 ETPHAFVA------LRADALPRTTGDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQKFVL 534
Cdd:PRK12467 949 DAGLQLVAylvpaaVADGAEHQATRDELKAQLRQVLPDYMVPAHlLLLDSLPLTPNGKLDRKAL 1012
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
34-529 |
7.96e-31 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 124.89 E-value: 7.96e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 34 PQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFIL 113
Cdd:cd17650 1 PDAIAVSDATRQLTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQYML 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 114 RHCAARVLICDREfgavaqqalamldappllvgidddqaeraDLAHdldyeaflaqgdpgrplsapqnewqsiaINYTSG 193
Cdd:cd17650 81 EDSGAKLLLTQPE-----------------------------DLAY----------------------------VIYTSG 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 194 TTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTL-SGGTHV-CLR--KVQPDAIHAAIAE 269
Cdd:cd17650 104 TTGKPKGVMVEHRNVAHAAHAWRREYELDSFPVRLLQMASFSFDVFAGDFARSLlNGGTLViCPDevKLDPAALYDLILK 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 270 HAVTHLSAAPVVMSMLIH--AEHASVPAVPVSVITGGAAPPSAVIAAMEAR---GFSITHAYGMTESYGPSTLclWQPGV 344
Cdd:cd17650 184 SRITLMESTPALIRPVMAyvYRNGLDLSAMRLLIVGSDGCKAQDFKTLAARfgqGMRIINSYGVTEATIDSTY--YEEGR 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 345 DELPleaRAQFMSrqgVAHPMLEEATVLDTDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAALADGWL------- 417
Cdd:cd17650 262 DPLG---DSANVP---IGRPLPNTAMYVLDERLQPQPVG--VAGELYIGGAGVARGYLNRPELTAERFVENPFapgermy 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 418 HTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVAlrADALPRTtgD 497
Cdd:cd17650 334 RTGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDEAVVAVREDKGGEARLCAYVV--AAATLNT--A 409
|
490 500 510
....*....|....*....|....*....|...
gi 489250734 498 DLVRWCRERLAHFKAPRH-VTLTELPKTATGKI 529
Cdd:cd17650 410 ELRAFLAKELPSYMIPSYyVQLDALPLTPNGKV 442
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
43-536 |
1.58e-30 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 125.50 E-value: 1.58e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 43 ARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRL-----EA--RSIAFILRH 115
Cdd:PRK09192 47 EEALPYQTLRARAEAGARRLLALGLKPGDRVALIAETDGDFVEAFFACQYAGLVPVPLPLPMgfggrESyiAQLRGMLAS 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 116 CAARVLIcdrefgavaqqalamldAPPLLVGIDDDQAERADLAHDLDYEAFLAQGDPGRPLSAPQNEwqSIA-INYTSGT 194
Cdd:PRK09192 127 AQPAAII-----------------TPDELLPWVNEATHGNPLLHVLSHAWFKALPEADVALPRPTPD--DIAyLQYSSGS 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 195 TGDPKGVVLHHRGAYLNACAGA---LIFQLGPRSIYlWtLPMFHCNG-----------------------------W--- 239
Cdd:PRK09192 188 TRFPRGVIITHRALMANLRAIShdgLKVRPGDRCVS-W-LPFYHDMGlvgflltpvatqlsvdylptrdfarrplqWldl 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 240 ----SHTWAVTLSGGTHVCLRKVqpdaihaaiaehavthlSAAPVVMSMLIHAEHASVPAVPVSvitggAAPPSAVIAAM 315
Cdd:PRK09192 266 isrnRGTISYSPPFGYELCARRV-----------------NSKDLAELDLSCWRVAGIGADMIR-----PDVLHQFAEAF 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 316 EARGFS---ITHAYGMTESygpsTLCLWQP----GVDELPLEARAqfMSRQGVAHPMLEEATVLDT--DTGRPVPA---- 382
Cdd:PRK09192 324 APAGFDdkaFMPSYGLAEA----TLAVSFSplgsGIVVEEVDRDR--LEYQGKAVAPGAETRRVRTfvNCGKALPGheie 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 383 ----DGRTLGELVV-----RGNTVMKGYLHNPEATRAALADGWLHTGDLAVLHpDGYVEIKDRAKDIIISGGENISSLEI 453
Cdd:PRK09192 398 irneAGMPLPERVVghicvRGPSLMSGYFRDEESQDVLAADGWLDTGDLGYLL-DGYLYITGRAKDLIIINGRNIWPQDI 476
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 454 EEVLYQHPEV----VEAAVVARPDsrwGETPHAFVALRA-DALPRTtgdDLVRWCRERL-AHFKAPRHVTLT---ELPKT 524
Cdd:PRK09192 477 EWIAEQEPELrsgdAAAFSIAQEN---GEKIVLLVQCRIsDEERRG---QLIHALAALVrSEFGVEAAVELVpphSLPRT 550
|
570
....*....|..
gi 489250734 525 ATGKIQKFVLRE 536
Cdd:PRK09192 551 SSGKLSRAKAKK 562
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
46-545 |
3.16e-29 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 121.55 E-value: 3.16e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 46 YSYRQLHERSCALASALERVGV--QAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLIC 123
Cdd:cd05927 6 ISYKEVAERADNIGSALRSLGGkpAPASFVGIYSINRPEWIISELACYAYSLVTVPLYDTLGPEAIEYILNHAEISIVFC 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 124 DREFGAVaqqalamldappllvgidddqaeradlahdlDYEAFLAQGDpGRPLSAPQNEWQSIA-INYTSGTTGDPKGVV 202
Cdd:cd05927 86 DAGVKVY-------------------------------SLEEFEKLGK-KNKVPPPPPKPEDLAtICYTSGTTGNPKGVM 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 203 LHHRgAYLNACAGALIF-----QLGPRSIYLWTLPMFH-----------CNGWSHTWavtLSGGTHVCLRKVQ---Pdai 263
Cdd:cd05927 134 LTHG-NIVSNVAGVFKIleilnKINPTDVYISYLPLAHifervvealflYHGAKIGF---YSGDIRLLLDDIKalkP--- 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 264 haaiaehavTHLSAAPVVMSMLIHAEHASVPAVP--------------------------------------------VS 299
Cdd:cd05927 207 ---------TVFPGVPRVLNRIYDKIFNKVQAKGplkrklfnfalnyklaelrsgvvraspfwdklvfnkikqalggnVR 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 300 VITGGAAPPSAVI-----AAMearGFSITHAYGMTESYGPSTLclwqpgvdelplearaqfmSRQGvahpmleeatvlDT 374
Cdd:cd05927 278 LMLTGSAPLSPEVleflrVAL---GCPVLEGYGQTECTAGATL-------------------TLPG------------DT 323
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 375 DTGR---PVPA------------------DGRtlGELVVRGNTVMKGYLHNPEATRAALA-DGWLHTGDLAVLHPDGYVE 432
Cdd:cd05927 324 SVGHvggPLPCaevklvdvpemnydakdpNPR--GEVCIRGPNVFSGYYKDPEKTAEALDeDGWLHTGDIGEWLPNGTLK 401
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 433 IKDRAKDII-ISGGENISSLEIEEVLYQHPEVVEAAVvarpdsrWGETPHAFvaLRADALPRTtgDDLVRWCRERlAHFK 511
Cdd:cd05927 402 IIDRKKNIFkLSQGEYVAPEKIENIYARSPFVAQIFV-------YGDSLKSF--LVAIVVPDP--DVLKEWAASK-GGGT 469
|
570 580 590
....*....|....*....|....*....|....
gi 489250734 512 APrHVTLTELPktatgKIQKFVLREWARQEEEAG 545
Cdd:cd05927 470 GS-FEELCKNP-----EVKKAILEDLVRLGKENG 497
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
30-531 |
4.80e-29 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 123.14 E-value: 4.80e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 30 AQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSI 109
Cdd:PRK12316 4561 ARMTPDAVAVVFDEEKLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGGAYVPLDPEYPRERL 4640
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 110 AFILRHCAARVLICDRefgavaqQALAMLDAPPLLVGIDDDQAEradlahdlDYEAFLAQgDPGRPLsAPQNEWQSIain 189
Cdd:PRK12316 4641 AYMMEDSGAALLLTQS-------HLLQRLPIPDGLASLALDRDE--------DWEGFPAH-DPAVRL-HPDNLAYVI--- 4700
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 190 YTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPmFHCNGWSHTWAVTLSGGTHVCLRKVQ---PDAIHAA 266
Cdd:PRK12316 4701 YTSGSTGRPKGVAVSHGSLVNHLHATGERYELTPDDRVLQFMS-FSFDGSHEGLYHPLINGASVVIRDDSlwdPERLYAE 4779
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 267 IAEHAVTHLSAAPVVMSMLIH--AEHASVPAVPVSVITGGAAPPSAViaameARGFSITHAYGMTESYGPStlclwQPGV 344
Cdd:PRK12316 4780 IHEHRVTVLVFPPVYLQQLAEhaERDGEPPSLRVYCFGGEAVAQASY-----DLAWRALKPVYLFNGYGPT-----ETTV 4849
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 345 DELPLEARAQFMSRQGVAH---PMLEEATVLDTDTGRPVPADGrtLGELVVRGNTVMKGYLHNPEATRA--------ALA 413
Cdd:PRK12316 4850 TVLLWKARDGDACGAAYMPigtPLGNRSGYVLDGQLNPLPVGV--AGELYLGGEGVARGYLERPALTAErfvpdpfgAPG 4927
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 414 DGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRwGETPHAFV----ALRAD 489
Cdd:PRK12316 4928 GRLYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIAQEGAV-GKQLVGYVvpqdPALAD 5006
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 489250734 490 ALPRTTG--DDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQK 531
Cdd:PRK12316 5007 ADEAQAElrDELKAALRERLPEYMVPAHlVFLARMPLTPNGKLDR 5051
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
34-529 |
1.28e-28 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 118.66 E-value: 1.28e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 34 PQRDAVVYGARRYSYRQLHERSCALASALERVG-VQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFI 112
Cdd:cd17648 1 PDRVAVVYGDKRLTYRELNERANRLAHYLLSVAeIRPDDLVGLVLDKSELMIIAILAVWKAGAAYVPIDPSYPDERIQFI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 113 LRHCAARVLIcdrefgavaqqalamldappllvgidddqAERADLAHdldyeaflaqgdpgrplsapqnewqsiaINYTS 192
Cdd:cd17648 81 LEDTGARVVI-----------------------------TNSTDLAY----------------------------AIYTS 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 193 GTTGDPKGVVLHHRGAyLNACAG--ALIFQLGPRS--IYLWTLPMFHCNGWSHTWAVtLSGGTHVCLR---KVQPDAIHA 265
Cdd:cd17648 104 GTTGKPKGVLVEHGSV-VNLRTSlsERYFGRDNGDeaVLFFSNYVFDFFVEQMTLAL-LNGQKLVVPPdemRFDPDRFYA 181
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 266 AIAEHAVTHLSAAPVVMSMLihaEHASVPAVPVsVITGGAAPPSAVIAAMEARgFS--ITHAYGMTESYGPSTlclwqpg 343
Cdd:cd17648 182 YINREKVTYLSGTPSVLQQY---DLARLPHLKR-VDAAGEEFTAPVFEKLRSR-FAglIINAYGPTETTVTNH------- 249
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 344 VDELPLEARAQFMSRQGVAHpmlEEATVLDTDTgRPVPADGrtLGELVVRGNTVMKGYLH-----------NPEATRAAL 412
Cdd:cd17648 250 KRFFPGDQRFDKSLGRPVRN---TKCYVLNDAM-KRVPVGA--VGELYLGGDGVARGYLNrpeltaerflpNPFQTEQER 323
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 413 ADG----WLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPH-----AF 483
Cdd:cd17648 324 ARGrnarLYKTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALASYPGVRECAVVAKEDASQAQSRIqkylvGY 403
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 489250734 484 VALRADALPRTtgdDLVRWCRERLAHFKAP-RHVTLTELPKTATGKI 529
Cdd:cd17648 404 YLPEPGHVPES---DLLSFLRAKLPRYMVPaRLVRLEGIPVTINGKL 447
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
188-529 |
1.32e-28 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 115.97 E-value: 1.32e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 188 INYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHcngwSHTWAVTLS----GGTHVCLRKVQPDAI 263
Cdd:cd17633 5 IGFTSGTTGLPKAYYRSERSWIESFVCNEDLFNISGEDAILAPGPLSH----SLFLYGAISalylGGTFIGQRKFNPKSW 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 264 HAAIAEHAVTHLSAAPVVMSMLIHAEhasVPAVPVSVITGGAAPPSAVIAAMEARGFSITHAYgmtESYGPSTLCLWQPG 343
Cdd:cd17633 81 IRKINQYNATVIYLVPTMLQALARTL---EPESKIKSIFSSGQKLFESTKKKLKNIFPKANLI---EFYGTSELSFITYN 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 344 VDElplearaqfmsrqgvahpmlEEATVLDTdtGRPVP--------ADGRTLGELVVRGNTVMKGYLHNPEATraalADG 415
Cdd:cd17633 155 FNQ--------------------ESRPPNSV--GRPFPnveieirnADGGEIGKIFVKSEMVFSGYVRGGFSN----PDG 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 416 WLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVAlrADALPRTT 495
Cdd:cd17633 209 WMSVGDIGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEIAVALYS--GDKLTYKQ 286
|
330 340 350
....*....|....*....|....*....|....*
gi 489250734 496 gddLVRWCRERLAHFKAPRH-VTLTELPKTATGKI 529
Cdd:cd17633 287 ---LKRFLKQKLSRYEIPKKiIFVDSLPYTSSGKI 318
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
190-528 |
7.51e-28 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 114.79 E-value: 7.51e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 190 YTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTL--------------PMFHCNGWShTWAVTLSGGTHVCL 255
Cdd:cd05924 10 YTGGTTGMPKGVMWRQEDIFRMLMGGADFGTGEFTPSEDAHKaaaaaagtvmfpapPLMHGTGSW-TAFGGLLGGQTVVL 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 256 --RKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVS----VITGGAAPPSAVIAAMEAR--GFSITHAYG 327
Cdd:cd05924 89 pdDRFDPEEVWRTIEKHKVTSMTIVGDAMARPLIDALRDAGPYDLSslfaISSGGALLSPEVKQGLLELvpNITLVDAFG 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 328 MTESYGPSTLClwqpgVDELPLEARaqfmSRQGVAHpmleEATVLDtDTGRPVPADGRTLGELVVRGNtVMKGYLHNPEA 407
Cdd:cd05924 169 SSETGFTGSGH-----SAGSGPETG----PFTRANP----DTVVLD-DDGRVVPPGSGGVGWIARRGH-IPLGYYGDEAK 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 408 TRAAL--ADG--WLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAF 483
Cdd:cd05924 234 TAETFpeVDGvrYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDERWGQEVVAV 313
|
330 340 350 360
....*....|....*....|....*....|....*....|....*.
gi 489250734 484 VALRADALPrtTGDDLVRWCRERLAHFKAPRHVTLT-ELPKTATGK 528
Cdd:cd05924 314 VQLREGAGV--DLEELREHCRTRIARYKLPKQVVFVdEIERSPAGK 357
|
|
| PLN02430 |
PLN02430 |
long-chain-fatty-acid-CoA ligase |
47-465 |
1.14e-27 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178049 [Multi-domain] Cd Length: 660 Bit Score: 117.61 E-value: 1.14e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 47 SYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVpgAGAVLVCINI--RLEARSIAFILRHCAAR-VLIC 123
Cdd:PLN02430 78 TYKEVYEEVLQIGSALRASGAEPGSRVGIYGSNCPQWIVAMEAC--AAHSLICVPLydTLGPGAVDYIVDHAEIDfVFVQ 155
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 124 DREFGAVAQQALAMLDAPPLLVGIDDDQAERADLAHDL-----DYEAFLAQGDPGRPLSAPQNEWQSIAINYTSGTTGDP 198
Cdd:PLN02430 156 DKKIKELLEPDCKSAKRLKAIVSFTSVTEEESDKASQIgvktySWIDFLHMGKENPSETNPPKPLDICTIMYTSGTSGDP 235
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 199 KGVVLHHRgAYLNACAGALIF------QLGPRSIYLWTLPMFH---------------CNGWSH-----------TWAVT 246
Cdd:PLN02430 236 KGVVLTHE-AVATFVRGVDLFmeqfedKMTHDDVYLSFLPLAHildrmieeyffrkgaSVGYYHgdlnalrddlmELKPT 314
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 247 LSGGTHVCLRKVQPDAIHAAIAEHAV--------------------THLSAAPVVmSMLIHAEHASVPAVPVSVITGGAA 306
Cdd:PLN02430 315 LLAGVPRVFERIHEGIQKALQELNPRrrlifnalykyklawmnrgySHKKASPMA-DFLAFRKVKAKLGGRLRLLISGGA 393
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 307 PPSAVIAAMeARGFS---ITHAYGMTESYGPSTLCLwqPgvDELPLearaqfMSRQGVA----HPMLEEATVLDTDT-GR 378
Cdd:PLN02430 394 PLSTEIEEF-LRVTScafVVQGYGLTETLGPTTLGF--P--DEMCM------LGTVGAPavynELRLEEVPEMGYDPlGE 462
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 379 PvPAdgrtlGELVVRGNTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDII-ISGGENIsSLEIEEVL 457
Cdd:PLN02430 463 P-PR-----GEICVRGKCLFSGYYKNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIkLSQGEYV-ALEYLENV 535
|
....*...
gi 489250734 458 YQHPEVVE 465
Cdd:PLN02430 536 YGQNPIVE 543
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
34-531 |
3.70e-27 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 114.49 E-value: 3.70e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 34 PQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFIL 113
Cdd:cd17656 2 PDAVAVVFENQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIM 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 114 RHCAARVLICDREFGAvaqqalaMLDAPPLLVGIDDDQAERADlAHDLDYEaflaqgdpgrplsapQNEWQSIAINYTSG 193
Cdd:cd17656 82 LDSGVRVVLTQRHLKS-------KLSFNKSTILLEDPSISQED-TSNIDYI---------------NNSDDLLYIIYTSG 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 194 TTGDPKGVVLHHRG---------AYLNACAGALIFQlgprsiylWTLPMFHCNgWSHTWAVTLSGGT-HVCLRKVQPDAI 263
Cdd:cd17656 139 TTGKPKGVQLEHKNmvnllhferEKTNINFSDKVLQ--------FATCSFDVC-YQEIFSTLLSGGTlYIIREETKRDVE 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 264 HAAI-AEHAVTHLSAAPVVMSMLIHAEHASVPAVPVSV---ITGGaappSAVIAAMEARGFSITHAYGMTESYGPS---- 335
Cdd:cd17656 210 QLFDlVKRHNIEVVFLPVAFLKFIFSEREFINRFPTCVkhiITAG----EQLVITNEFKEMLHEHNVHLHNHYGPSethv 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 336 -TLCLWQPGVD--ELPlearaqfmsrqGVAHPMLEEATVLDTDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAAL 412
Cdd:cd17656 286 vTTYTINPEAEipELP-----------PIGKPISNTWIYILDQEQQLQPQG--IVGELYISGASVARGYLNRQELTAEKF 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 413 ------ADGWLH-TGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVA 485
Cdd:cd17656 353 fpdpfdPNERMYrTGDLARYLPDGNIEFLGRADHQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKGEKYLCAYFV 432
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 489250734 486 lradALPRTTGDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQK 531
Cdd:cd17656 433 ----MEQELNISQLREYLAKQLPEYMIPSFfVPLDQLPLTPNGKVDR 475
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
30-534 |
7.32e-27 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 116.21 E-value: 7.32e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 30 AQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSI 109
Cdd:PRK12316 521 VERTPEAPALAFGEETLDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEYPAERL 600
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 110 AFILRHCAARVLICDRefgavaqqalAMLDAPPLLVGIDddqaeradlAHDLDYEAFLAQGDPGRPLSAPQNEWQSIAIN 189
Cdd:PRK12316 601 AYMLEDSGVQLLLSQS----------HLGRKLPLAAGVQ---------VLDLDRPAAWLEGYSEENPGTELNPENLAYVI 661
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 190 YTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPM-FHCNGWSHTWAVTLSGGTHVCLRKV--QPDAIHAA 266
Cdd:PRK12316 662 YTSGSTGKPKGAGNRHRALSNRLCWMQQAYGLGVGDTVLQKTPFsFDVSVWEFFWPLMSGARLVVAAPGDhrDPAKLVEL 741
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 267 IAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVSVITGGAAPPSAVIAAMEARgFSITHAYGMtesYGPS----TLCLW-- 340
Cdd:PRK12316 742 INREGVDTLHFVPSMLQAFLQDEDVASCTSLRRIVCSGEALPADAQEQVFAK-LPQAGLYNL---YGPTeaaiDVTHWtc 817
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 341 -QPGVDELPlearaqfmsrqgVAHPMLEEAT-VLDTDtGRPVPADgrTLGELVVRGNTVMKGYLHNPEATR-----AALA 413
Cdd:PRK12316 818 vEEGGDSVP------------IGRPIANLACyILDAN-LEPVPVG--VLGELYLAGRGLARGYHGRPGLTAerfvpSPFV 882
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 414 DG--WLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPdsrwGETPHAFVALRADAl 491
Cdd:PRK12316 883 AGerMYRTGDLARYRADGVIEYAGRIDHQVKLRGLRIELGEIEARLLEHPWVREAAVLAVD----GKQLVGYVVLESEG- 957
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 489250734 492 pRTTGDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQKFVL 534
Cdd:PRK12316 958 -GDWREALKAHLAASLPEYMVPAQwLALERLPLTPNGKLDRKAL 1000
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
44-471 |
1.89e-26 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 113.29 E-value: 1.89e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 44 RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLIC 123
Cdd:cd17641 10 QEFTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 124 DREfgavaQQALAMLDAPPLLVGID---------------------DDQAERADLAHDLD---YEAFLAQGDPGrplsap 179
Cdd:cd17641 90 EDE-----EQVDKLLEIADRIPSVRyviycdprgmrkyddprlisfEDVVALGRALDRRDpglYEREVAAGKGE------ 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 180 qnewqSIAI-NYTSGTTGDPKGVVLHHRgAYLNACAGALIFQ-LGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVC--- 254
Cdd:cd17641 159 -----DVAVlCTTSGTTGKPKLAMLSHG-NFLGHCAAYLAADpLGPGDEYVSVLPLPWIGEQMYSVGQALVCGFIVNfpe 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 255 --------LRKVQPDAIHAA-------IAEHAVTHLSAAPV-------VMSMLIHAEHASVPAVPV-------------- 298
Cdd:cd17641 233 epetmmedLREIGPTFVLLPprvwegiAADVRARMMDATPFkrfmfelGMKLGLRALDRGKRGRPVslwlrlaswladal 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 299 ---------------SVITGGAAPPSAVIAAMEARGFSITHAYGMTESYGPSTLclwQPGVDelplearaqfmsrqgvah 363
Cdd:cd17641 313 lfrplrdrlgfsrlrSAATGGAALGPDTFRFFHAIGVPLKQLYGQTELAGAYTV---HRDGD------------------ 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 364 pmleeatvLDTDT-GRPVP-ADGR--TLGELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAK 438
Cdd:cd17641 372 --------VDPDTvGVPFPgTEVRidEVGEILVRSPGVFVGYYKNPEATAEDFdEDGWLHTGDAGYFKENGHLVVIDRAK 443
|
490 500 510
....*....|....*....|....*....|....
gi 489250734 439 DI-IISGGENISSLEIEEVLYQHPEVVEAAVVAR 471
Cdd:cd17641 444 DVgTTSDGTRFSPQFIENKLKFSPYIAEAVVLGA 477
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
33-535 |
3.64e-26 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 113.20 E-value: 3.64e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 33 YPQRDAVVYGarrysyrQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFI 112
Cdd:PRK06060 25 FYAADVVTHG-------QIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLANPELHRDDHALA 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 113 LRHCAARVLI-----CDRefgavaQQALAMLDAPPLLvgiddDQAERADLAhdlDYEaflaqgdpgrPLSAPQNEWQSia 187
Cdd:PRK06060 98 ARNTEPALVVtsdalRDR------FQPSRVAEAAELM-----SEAARVAPG---GYE----------PMGGDALAYAT-- 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 188 inYTSGTTGDPKGVVLHHRG--AYLNA-CAGALifQLGPRSIYLWTLPMFHCNGWSHT-WAVTLSGGTHVCLR-KVQPDA 262
Cdd:PRK06060 152 --YTSGTTGPPKAAIHRHADplTFVDAmCRKAL--RLTPEDTGLCSARMYFAYGLGNSvWFPLATGGSAVINSaPVTPEA 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 263 IHAAIAEHAVTHLSAAPVVMSMLIHAEHA-SVPAVPVSVITGGAAPPSAVIAAMEA-RGFSITHAYGMTE---SYGPSTL 337
Cdd:PRK06060 228 AAILSARFGPSVLYGVPNFFARVIDSCSPdSFRSLRCVVSAGEALELGLAERLMEFfGGIPILDGIGSTEvgqTFVSNRV 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 338 CLWQPGvdelplearaqfmsrqgvahpmlEEATVLDTDTGRPVPADGRTLG-----ELVVRGNTVMKGYLHNPEATraaL 412
Cdd:PRK06060 308 DEWRLG-----------------------TLGRVLPPYEIRVVAPDGTTAGpgvegDLWVRGPAIAKGYWNRPDSP---V 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 413 AD-GWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAF-VALRADA 490
Cdd:PRK06060 362 ANeGWLDTRDRVCIDSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFlVATSGAT 441
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 489250734 491 LPRTTGDDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKIQKFVLR 535
Cdd:PRK06060 442 IDGSVMRDLHRGLLNRLSAFKVPHRFAVVDrLPRTPNGKLVRGALR 487
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
42-473 |
4.73e-26 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 111.54 E-value: 4.73e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 42 GARRY-SYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARV 120
Cdd:cd17639 1 GEYKYmSYAEVWERVLNFGRGLVELGLKPGDKVAIFAETRAEWLITALGCWSQNIPIVTVYATLGEDALIHSLNETECSA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 121 LICDrefgavaqqalamldappllvGIDDDQAeradlahdldyeaflaqgdpgrplsapqnewqsiAINYTSGTTGDPKG 200
Cdd:cd17639 81 IFTD---------------------GKPDDLA----------------------------------CIMYTSGSTGNPKG 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 201 VVLHHRG--AYLNACAGALIFQLGPRSIYLWTLPMFH---------------CNGWSHtwAVTLSG----GTHVCLRKVQ 259
Cdd:cd17639 106 VMLTHGNlvAGIAGLGDRVPELLGPDDRYLAYLPLAHifelaaenvclyrggTIGYGS--PRTLTDkskrGCKGDLTEFK 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 260 PdaihaaiaehavTHLSAAPVVMSMLIHAEHASVPAVP------------------------------------------ 297
Cdd:cd17639 184 P------------TLMVGVPAIWDTIRKGVLAKLNPMGglkrtlfwtayqsklkalkegpgtplldelvfkkvraalggr 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 298 VSVITGGAAPPSA-------VIAAMEARGfsithaYGMTESYGPSTLCLWqpgvDELplearaqfmsRQGVAHPML--EE 368
Cdd:cd17639 252 LRYMLSGGAPLSAdtqeflnIVLCPVIQG------YGLTETCAGGTVQDP----GDL----------ETGRVGPPLpcCE 311
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 369 ATVLDTDTGRPVPADGRTLGELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDII-ISGGE 446
Cdd:cd17639 312 IKLVDWEEGGYSTDKPPPRGEILIRGPNVFKGYYKNPEKTKEAFdGDGWFHTGDIGEFHPDGTLKIIDRKKDLVkLQNGE 391
|
490 500
....*....|....*....|....*...
gi 489250734 447 NIsSLE-IEEVLYQHPEVVEAAVVARPD 473
Cdd:cd17639 392 YI-ALEkLESIYRSNPLVNNICVYADPD 418
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
44-536 |
4.85e-26 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 110.91 E-value: 4.85e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 44 RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLIC 123
Cdd:cd05940 2 EALTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLVV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 124 DRefgavaqqalamldappllvgidddqaeradlahdldyeAFLAqgdpgrplsapqnewqsiainYTSGTTGDPK-GVV 202
Cdd:cd05940 82 DA---------------------------------------ALYI---------------------YTSGTTGLPKaAII 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 203 LHHRGAYLNACAGALIFQLGPRSIYLwTLPMFHCNGWSHTWAVTLSGGTHVCLRK------VQPDaihaaiaehaVTHLS 276
Cdd:cd05940 102 SHRRAWRGGAFFAGSGGALPSDVLYT-CLPLYHSTALIVGWSACLASGATLVIRKkfsasnFWDD----------IRKYQ 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 277 AAPVVMSMLIHAEHASVPAVP------VSVITGGAAPPSaVIAAMEARgFSITHaygMTESYGPS--TLCLWQ-PGVDel 347
Cdd:cd05940 171 ATIFQYIGELCRYLLNQPPKPterkhkVRMIFGNGLRPD-IWEEFKER-FGVPR---IAEFYAATegNSGFINfFGKP-- 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 348 pleaRAQFMSRQGVAHPMLEEATVLDTDTGRPV-PADGRTL-------GELVVRGNTV--MKGYLHNPEAT----RAALA 413
Cdd:cd05940 244 ----GAIGRNPSLLRKVAPLALVKYDLESGEPIrDAEGRCIkvprgepGLLISRINPLepFDGYTDPAATEkkilRDVFK 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 414 DG--WLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAV----VARPDSRWGetphaFVALR 487
Cdd:cd05940 320 KGdaWFNTGDLMRLDGEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVygvqVPGTDGRAG-----MAAIV 394
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 489250734 488 ADALPRTTGDDLVRWCRERLAHFKAPRHVTLT-ELPKTATGKIQKFVLRE 536
Cdd:cd05940 395 LQPNEEFDLSALAAHLEKNLPGYARPLFLRLQpEMEITGTFKQQKVDLRN 444
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
30-538 |
5.37e-26 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 111.10 E-value: 5.37e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 30 AQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRL-EARs 108
Cdd:cd05918 9 ARSQPDAPAVCAWDGSLTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGGAFVPLDPSHpLQR- 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 109 IAFILRHCAARVLICDRefgavaqqalamldappllvgidddqaeradlAHDLdyeAFLAqgdpgrplsapqnewqsiai 188
Cdd:cd05918 88 LQEILQDTGAKVVLTSS--------------------------------PSDA---AYVI-------------------- 112
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 189 nYTSGTTGDPKGVVLHHRgaylNACAGAL----IFQLGPRSIYLWtlpmFHcngwSHTWAVTL--------SGGThVCL- 255
Cdd:cd05918 113 -FTSGSTGKPKGVVIEHR----ALSTSALahgrALGLTSESRVLQ----FA----SYTFDVSIleifttlaAGGC-LCIp 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 256 -RKVQPDAIHAAIAEHAVTHLSAAPVVMSMLihaEHASVPAVPVsVITGGAAPPSAVIAAMeARGFSITHAYGMTESygp 334
Cdd:cd05918 179 sEEDRLNDLAGFINRLRVTWAFLTPSVARLL---DPEDVPSLRT-LVLGGEALTQSDVDTW-ADRVRLINAYGPAEC--- 250
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 335 STLCLWQPgvdeLPLEARAQFMSR--QGVAHpmleeatVLDTDT-GRPVPADGrtLGELVVRGNTVMKGYLHNPEATRAA 411
Cdd:cd05918 251 TIAATVSP----VVPSTDPRNIGRplGATCW-------VVDPDNhDRLVPIGA--VGELLIEGPILARGYLNDPEKTAAA 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 412 --LADGWLH------------TGDLAVLHPDGYVEIKDRaKD--IIISG-----GenisslEIEEVLYQHPEVVEAAVVA 470
Cdd:cd05918 318 fiEDPAWLKqegsgrgrrlyrTGDLVRYNPDGSLEYVGR-KDtqVKIRGqrvelG------EIEHHLRQSLPGAKEVVVE 390
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 471 --RPDSRwGETPH--AFVALRADALPRTTGDDLV---------------RWCRERLAHFKAPRH-VTLTELPKTATGKIQ 530
Cdd:cd05918 391 vvKPKDG-SSSPQlvAFVVLDGSSSGSGDGDSLFlepsdefralvaelrSKLRQRLPSYMVPSVfLPLSHLPLTASGKID 469
|
....*...
gi 489250734 531 KFVLREWA 538
Cdd:cd05918 470 RRALRELA 477
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
41-536 |
6.70e-25 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 107.90 E-value: 6.70e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 41 YGARRYSYRQLHERSCALASAL-ERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAAR 119
Cdd:cd05937 1 FEGKTWTYSETYDLVLRYAHWLhDDLGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAAPAFINYNLSGDPLIHCLKLSGSR 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 120 VLICDrefgavaqqalamldappllvgiDDDQAeradlahdldyeaflaqgdpgrplsapqnewqsiAINYTSGTTGDPK 199
Cdd:cd05937 81 FVIVD-----------------------PDDPA----------------------------------ILIYTSGTTGLPK 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 200 GVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCL-RKVQPDAIHAAIAEHAVTHLSAA 278
Cdd:cd05937 104 AAAISWRRTLVTSNLLSHDLNLKNGDRTYTCMPLYHGTAAFLGACNCLMSGGTLALsRKFSASQFWKDVRDSGATIIQYV 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 279 PVVMSMLIHAehasvPAVP------VSVITGGAAPPSAVIAAMEARGFSITHA-YGMTEsyGPSTLCLWQPGvdelPLEA 351
Cdd:cd05937 184 GELCRYLLST-----PPSPydrdhkVRVAWGNGLRPDIWERFRERFNVPEIGEfYAATE--GVFALTNHNVG----DFGA 252
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 352 RAqfMSRQG-VAHPMLEEATVL---DTDTGRPV--PADG-------RTLGELVVRGN----TVMKGYLHNPEATRAAL-- 412
Cdd:cd05937 253 GA--IGHHGlIRRWKFENQVVLvkmDPETDDPIrdPKTGfcvrapvGEPGEMLGRVPfknrEAFQGYLHNEDATESKLvr 330
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 413 -----ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAV----VARPDSRWGetpHAF 483
Cdd:cd05937 331 dvfrkGDIYFRTGDLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADVLGAHPDIAEANVygvkVPGHDGRAG---CAA 407
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 489250734 484 VALRADALPRTTGD--DLVRWCRERLAHFKAPRHVTLT-ELPKTATGKIQKFVLRE 536
Cdd:cd05937 408 ITLEESSAVPTEFTksLLASLARKNLPSYAVPLFLRLTeEVATTDNHKQQKGVLRD 463
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
25-531 |
1.53e-24 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 109.10 E-value: 1.53e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 25 FLKRAAQVyPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRL 104
Cdd:PRK12467 1580 IEDQAAAT-PEAVALVFGEQELTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEY 1658
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 105 EARSIAFILRHCAARVLICDREfgavAQQALAMLDAPPLLVgidddqaeradlahdLDYE-AFLAQGDPGRPLSAPQNEW 183
Cdd:PRK12467 1659 PRERLAYMIEDSGIELLLTQSH----LQARLPLPDGLRSLV---------------LDQEdDWLEGYSDSNPAVNLAPQN 1719
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 184 QSIAInYTSGTTGDPKGVVLHHrGAYLNA-CAGALIFQLGPRSIYLWTLPM-FHCNGWSHTWAVtLSGGTHVCLR---KV 258
Cdd:PRK12467 1720 LAYVI-YTSGSTGRPKGAGNRH-GALVNRlCATQEAYQLSAADVVLQFTSFaFDVSVWELFWPL-INGARLVIAPpgaHR 1796
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 259 QPDAIHAAIAEHAVTHLSAAPVVMSMLI-HAEH-ASVPAVPVSVITGGAAPPSAVIAAMEARGFS-ITHAYGMTESygPS 335
Cdd:PRK12467 1797 DPEQLIQLIERQQVTTLHFVPSMLQQLLqMDEQvEHPLSLRRVVCGGEALEVEALRPWLERLPDTgLFNLYGPTET--AV 1874
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 336 TLCLWQpgVDELPLEARAQfmsrQGVAHPMLEEATVLDTDTGRPVPAdgRTLGELVVRGNTVMKGYLHNPEATRA----- 410
Cdd:PRK12467 1875 DVTHWT--CRRKDLEGRDS----VPIGQPIANLSTYILDASLNPVPI--GVAGELYLGGVGLARGYLNRPALTAErfvad 1946
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 411 --ALADGWLH-TGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARpDSRWGETPHAFVALR 487
Cdd:PRK12467 1947 pfGTVGSRLYrTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLREQGGVREAVVIAQ-DGANGKQLVAYVVPT 2025
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 489250734 488 ADALPrtTGDDLVRWCRERL-AHFKA-------PRH-VTLTELPKTATGKIQK 531
Cdd:PRK12467 2026 DPGLV--DDDEAQVALRAILkNHLKAslpeymvPAHlVFLARMPLTPNGKLDR 2076
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
25-529 |
1.55e-24 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 108.47 E-value: 1.55e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 25 FLKRAAQVyPQRDAVV-YGARRYSYRQLHERSCALASALERvGVQAGERVAILAP-NIPEMLeAHYGVPGAGAVLVCINI 102
Cdd:PRK08633 621 WIDTAKRN-WSRLAVAdSTGGELSYGKALTGALALARLLKR-ELKDEENVGILLPpSVAGAL-ANLALLLAGKVPVNLNY 697
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 103 RLEARSIAFILRHCAARVLICDREFGAVAQQALAMLDAPP---------LLVGIDDDQAERADLAhdldyeAFLAqgdPG 173
Cdd:PRK08633 698 TASEAALKSAIEQAQIKTVITSRKFLEKLKNKGFDLELPEnvkviyledLKAKISKVDKLTALLA------ARLL---PA 768
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 174 RPLSAPQNEWQSI----AINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHT-WAVTLS 248
Cdd:PRK08633 769 RLLKRLYGPTFKPddtaTIIFSSGSEGEPKGVMLSHHNILSNIEQISDVFNLRNDDVILSSLPFFHSFGLTVTlWLPLLE 848
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 249 GGTHVClrkvQPD-----AIHAAIAEHAVTHLSAAPVVMSMLIHAEHASvPAVPVS---VITGGAAPPSAVIAAMEAR-G 319
Cdd:PRK08633 849 GIKVVY----HPDptdalGIAKLVAKHRATILLGTPTFLRLYLRNKKLH-PLMFASlrlVVAGAEKLKPEVADAFEEKfG 923
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 320 FSITHAYGMTESYGPSTLCLwqPgvDELPLEARAQFMSRQG-VAHPMLEEAT-VLDTDTGRPVPADgrTLGELVVRGNTV 397
Cdd:PRK08633 924 IRILEGYGATETSPVASVNL--P--DVLAADFKRQTGSKEGsVGMPLPGVAVrIVDPETFEELPPG--EDGLILIGGPQV 997
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 398 MKGYLHNPEATRAALAD----GWLHTGDLAVLHPDGYVEIKDR----AKdIiisGGENISSLEIEEVLYQ--HPEVVEAA 467
Cdd:PRK08633 998 MKGYLGDPEKTAEVIKDidgiGWYVTGDKGHLDEDGFLTITDRysrfAK-I---GGEMVPLGAVEEELAKalGGEEVVFA 1073
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 489250734 468 VVARPDSRWGE---TPHAFVALRADALprttgddlvrwcRERLAH------FKAPRHVTLTELPKTATGKI 529
Cdd:PRK08633 1074 VTAVPDEKKGEklvVLHTCGAEDVEEL------------KRAIKEsglpnlWKPSRYFKVEALPLLGSGKL 1132
|
|
| ACSBG_like |
cd05933 |
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ... |
47-455 |
2.93e-24 |
|
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341256 [Multi-domain] Cd Length: 596 Bit Score: 106.67 E-value: 2.93e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 47 SYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDRe 126
Cdd:cd05933 10 TYKEYYEACRQAAKAFLKLGLERFHGVGILGFNSPEWFIAAVGAIFAGGIAVGIYTTNSPEACQYVAETSEANILVVEN- 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 127 fgavAQQA---LAMLDAPPLLVGI----DDDQAERADLahdLDYEAFLAQGDpGRPLSAPQNEWQSIAIN------YTSG 193
Cdd:cd05933 89 ----QKQLqkiLQIQDKLPHLKAIiqykEPLKEKEPNL---YSWDEFMELGR-SIPDEQLDAIISSQKPNqcctliYTSG 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 194 TTGDPKGVVLHHRGAYLNACAGALIFQLGPRSI-------YL------------WTlPMFHCNGWSHTWAVTLSGGTHVC 254
Cdd:cd05933 161 TTGMPKGVMLSHDNITWTAKAASQHMDLRPATVgqesvvsYLplshiaaqildiWL-PIKVGGQVYFAQPDALKGTLVKT 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 255 LRKVQPDA------------IHAAIAEHAVTHLSAAPVVMSMLIHAEHASV-------PAVP------------------ 297
Cdd:cd05933 240 LREVRPTAfmgvprvwekiqEKMKAVGAKSGTLKRKIASWAKGVGLETNLKlmggespSPLFyrlakklvfkkvrkalgl 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 298 ---VSVITGGAAPPSAVIAAMEARGFSITHAYGMTESYGPSTLCLWQpgvdelplearAQFMSRQGVAHPMLEeaTVLDT 374
Cdd:cd05933 320 drcQKFFTGAAPISRETLEFFLSLNIPIMELYGMSETSGPHTISNPQ-----------AYRLLSCGKALPGCK--TKIHN 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 375 DTgrpvpADGrtLGELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIII-SGGENISSLE 452
Cdd:cd05933 387 PD-----ADG--IGEICFWGRHVFMGYLNMEDKTEEAIdEDGWLHSGDLGKLDEDGFLYITGRIKELIItAGGENVPPVP 459
|
...
gi 489250734 453 IEE 455
Cdd:cd05933 460 IED 462
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
34-534 |
4.57e-24 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 107.55 E-value: 4.57e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 34 PQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFIL 113
Cdd:PRK12467 3109 PEAPALVFGDQQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGAYVPLDPEYPRERLAYMI 3188
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 114 RHCAARVLIcdrefgavAQQALamLDAPPLLVGidddqaeraDLAHDLDyeaflaQGDP-GRPLSAPQN--EWQSIA-IN 189
Cdd:PRK12467 3189 EDSGVKLLL--------TQAHL--LEQLPAPAG---------DTALTLD------RLDLnGYSENNPSTrvMGENLAyVI 3243
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 190 YTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPmFHCNGWSHTWAVTLSGGTHVCLRKVQ---PDAIHAA 266
Cdd:PRK12467 3244 YTSGSTGKPKGVGVRHGALANHLCWIAEAYELDANDRVLLFMS-FSFDGAQERFLWTLICGGCLVVRDNDlwdPEELWQA 3322
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 267 IAEHAVTHLSAAP-VVMSMLIHAEHASVPAVPVSVITGGAAPPSA---VIAAMEARGfsITHAYGMTESYGPSTLclWQP 342
Cdd:PRK12467 3323 IHAHRISIACFPPaYLQQFAEDAGGADCASLDIYVFGGEAVPPAAfeqVKRKLKPRG--LTNGYGPTEAVVTVTL--WKC 3398
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 343 GVDELPLEARAQfmsrqgVAHPMLEEATVLDTDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAA-LAD------G 415
Cdd:PRK12467 3399 GGDAVCEAPYAP------IGRPVAGRSIYVLDGQLNPVPVG--VAGELYIGGVGLARGYHQRPSLTAERfVADpfsgsgG 3470
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 416 WLH-TGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARpDSRWGETPHAFVALraDALPRT 494
Cdd:PRK12467 3471 RLYrTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLLQHPSVREAVVLAR-DGAGGKQLVAYVVP--ADPQGD 3547
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 489250734 495 TGDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQKFVL 534
Cdd:PRK12467 3548 WRETLRDHLAASLPDYMVPAQlLVLAAMPLGPNGKVDRKAL 3588
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
47-540 |
1.18e-23 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 105.82 E-value: 1.18e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 47 SYRQLHERSCALASALERvGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLearSIAFILRHCAA---RVLIC 123
Cdd:PRK06814 660 TYRKLLTGAFVLGRKLKK-NTPPGENVGVMLPNANGAAVTFFALQSAGRVPAMINFSA---GIANILSACKAaqvKTVLT 735
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 124 DREFgaVAQqalAMLDapPLLVGID--------DDQAERADLAHDLDyeAFLAQGDPGRPLSAPQNEwQSIAINYTSGTT 195
Cdd:PRK06814 736 SRAF--IEK---ARLG--PLIEALEfgiriiylEDVRAQIGLADKIK--GLLAGRFPLVYFCNRDPD-DPAVILFTSGSE 805
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 196 GDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGwshtwavtLSGGThvclrkvqpdaihaaiaehAVTHL 275
Cdd:PRK06814 806 GTPKGVVLSHRNLLANRAQVAARIDFSPEDKVFNALPVFHSFG--------LTGGL-------------------VLPLL 858
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 276 SAAPVVM----------SMLIHAEHASV-------------PAVP-----VSVITGGAAPpsaVIAA-----MEARGFSI 322
Cdd:PRK06814 859 SGVKVFLypsplhyriiPELIYDTNATIlfgtdtflngyarYAHPydfrsLRYVFAGAEK---VKEEtrqtwMEKFGIRI 935
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 323 THAYGMTESyGPSTLClwqpgvdELPLEARAQFMSRqgvAHPMLEeaTVLDtdtgrPVPA--DGrtlGELVVRGNTVMKG 400
Cdd:PRK06814 936 LEGYGVTET-APVIAL-------NTPMHNKAGTVGR---LLPGIE--YRLE-----PVPGidEG---GRLFVRGPNVMLG 994
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 401 YLH--NPEATRAaLADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVL------YQHpevveaAVVARP 472
Cdd:PRK06814 995 YLRaeNPGVLEP-PADGWYDTGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVEELAaelwpdALH------AAVSIP 1067
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 489250734 473 DSRWGETPHAFvalradalprTTGDDLVRwcRERLAHFKA--------PRHV-TLTELPKTATGKIQKFVLREWARQ 540
Cdd:PRK06814 1068 DARKGERIILL----------TTASDATR--AAFLAHAKAagaselmvPAEIiTIDEIPLLGTGKIDYVAVTKLAEE 1132
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
30-534 |
1.21e-23 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 103.79 E-value: 1.21e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 30 AQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSI 109
Cdd:cd17645 8 VERTPDHVAVVDRGQSLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPDYPGERI 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 110 AFILRHCAARVLICDREfgavaqqalamldappllvgidddqaeraDLAHdldyeaflaqgdpgrplsapqnewqsiaIN 189
Cdd:cd17645 88 AYMLADSSAKILLTNPD-----------------------------DLAY----------------------------VI 110
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 190 YTSGTTGDPKGVVLHHRgAYLNACAGAL-IFQLGPR---SIYlwTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPDAiha 265
Cdd:cd17645 111 YTSGSTGLPKGVMIEHH-NLVNLCEWHRpYFGVTPAdksLVY--ASFSFDASAWEIFPHLTAGAALHVVPSERRLDL--- 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 266 aiaehavthlsaapVVMSMLIHAEHASVPAVPV---------------SVITGGAappsaVIAAMEARGFSITHAYGMTE 330
Cdd:cd17645 185 --------------DALNDYFNQEGITISFLPTgaaeqfmqldnqslrVLLTGGD-----KLKKIERKGYKLVNNYGPTE 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 331 SYGPSTLCLWQPGVDELPlearaqfmsrqgVAHPmLEEATVLDTDTGRPVPADGRTlGELVVRGNTVMKGYLHNPEATRA 410
Cdd:cd17645 246 NTVVATSFEIDKPYANIP------------IGKP-IDNTRVYILDEALQLQPIGVA-GELCIAGEGLARGYLNRPELTAE 311
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 411 AL-------ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAF 483
Cdd:cd17645 312 KFivhpfvpGERMYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDADGRKYLVAY 391
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 489250734 484 VALRADALPrttgDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQKFVL 534
Cdd:cd17645 392 VTAPEEIPH----EELREWLKNDLPDYMIPTYfVHLKALPLTANGKVDRKAL 439
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
30-534 |
2.89e-23 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 105.04 E-value: 2.89e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 30 AQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSI 109
Cdd:PRK12316 3067 VERTPDAVALAFGEQRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEERL 3146
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 110 AFILRHCAARVLICDREFGAVAQQALAMLDAPPllvgiDDDQAERADLAHDLDyeaflaqgdpgrplsaPQNEWQSIain 189
Cdd:PRK12316 3147 AYMLEDSGAQLLLSQSHLRLPLAQGVQVLDLDR-----GDENYAEANPAIRTM----------------PENLAYVI--- 3202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 190 YTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLwTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQ----PDAIHA 265
Cdd:PRK12316 3203 YTSGSTGKPKGVGIRHSALSNHLCWMQQAYGLGVGDRVL-QFTTFSFDVFVEELFWPLMSGARVVLAGPEdwrdPALLVE 3281
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 266 AIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVSVITGGAAPPSAVIAAMEArGFSITHAYGMTES-YGPSTLCLWQPGV 344
Cdd:PRK12316 3282 LINSEGVDVLHAYPSMLQAFLEEEDAHRCTSLKRIVCGGEALPADLQQQVFA-GLPLYNLYGPTEAtITVTHWQCVEEGK 3360
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 345 DELPlearaqfmsrqgVAHPMLEEATVLDTDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRA-------ALADGWL 417
Cdd:PRK12316 3361 DAVP------------IGRPIANRACYILDGSLEPVPVG--ALGELYLGGEGLARGYHNRPGLTAErfvpdpfVPGERLY 3426
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 418 HTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPdsrwGETPHAFVALRAD--ALPRTT 495
Cdd:PRK12316 3427 RTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPWVREAVVLAVD----GRQLVAYVVPEDEagDLREAL 3502
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 489250734 496 GDDLvrwcRERLAHFKAPRH-VTLTELPKTATGKIQKFVL 534
Cdd:PRK12316 3503 KAHL----KASLPEYMVPAHlLFLERMPLTPNGKLDRKAL 3538
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
44-546 |
8.02e-23 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 102.53 E-value: 8.02e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 44 RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEmleahygvpGAGAVLVCinirleAR-----SIAF------I 112
Cdd:PRK00174 97 RKITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPE---------AAVAMLAC------ARigavhSVVFggfsaeA 161
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 113 LRH----CAARVLICDREF---G------AVAQQALAMldAPPL---LV----GIDDDQAERADLA-HDL-----DY--- 163
Cdd:PRK00174 162 LADriidAGAKLVITADEGvrgGkpiplkANVDEALAN--CPSVekvIVvrrtGGDVDWVEGRDLWwHELvagasDEcep 239
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 164 EAFLAQgDPgrplsapqnewqsIAINYTSGTTGDPKGVvLHHRGAYLnacAGAL-----IFQLGPRSIYlWtlpmfhCN- 237
Cdd:PRK00174 240 EPMDAE-DP-------------LFILYTSGSTGKPKGV-LHTTGGYL---VYAAmtmkyVFDYKDGDVY-W------CTa 294
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 238 --GW--SHTWAV---TLSGGTHVCLRKV----QPDAIHAAIAEHAVTHLSAAPVVMSMLI-HAEHA-------------S 292
Cdd:PRK00174 295 dvGWvtGHSYIVygpLANGATTLMFEGVpnypDPGRFWEVIDKHKVTIFYTAPTAIRALMkEGDEHpkkydlsslrllgS 374
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 293 V--PAVPVS------VITGGAAPpsavIA----AMEARGFSITHAYGMTESygpstlclwQPGVDELPLEaraqfmsrqG 360
Cdd:PRK00174 375 VgePINPEAwewyykVVGGERCP----IVdtwwQTETGGIMITPLPGATPL---------KPGSATRPLP---------G 432
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 361 VahpmleEATVLDtDTGRPVPADGRtlGELVVR----GntVMKGYLHNPE---ATRAALADGWLHTGDLAVLHPDGYVEI 433
Cdd:PRK00174 433 I------QPAVVD-EEGNPLEGGEG--GNLVIKdpwpG--MMRTIYGDHErfvKTYFSTFKGMYFTGDGARRDEDGYYWI 501
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 434 KDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALP-RTTGDDLVRWCRERLAHFKA 512
Cdd:PRK00174 502 TGRVDDVLNVSGHRLGTAEIESALVAHPKVAEAAVVGRPDDIKGQGIYAFVTLKGGEEPsDELRKELRNWVRKEIGPIAK 581
|
570 580 590
....*....|....*....|....*....|....*
gi 489250734 513 PRHVTLT-ELPKTATGKIQKFVLREWARQEEEAGD 546
Cdd:PRK00174 582 PDVIQFApGLPKTRSGKIMRRILRKIAEGEEILGD 616
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
42-531 |
1.33e-22 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 101.23 E-value: 1.33e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 42 GARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCinirlearsiafiLRHCAARVl 121
Cdd:PRK07768 26 APVRHTWGEVHERARRIAGGLAAAGVGPGDAVAVLAGAPVEIAPTAQGLWMRGASLTM-------------LHQPTPRT- 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 122 icdrEFGAVAQQ---ALAMLDAPPLLVGIDDDQAERADLAHDLDYEAF--LAQGDPGRPLSAPQNewqSIAI-NYTSGTT 195
Cdd:PRK07768 92 ----DLAVWAEDtlrVIGMIGAKAVVVGEPFLAAAPVLEEKGIRVLTVadLLAADPIDPVETGED---DLALmQLTSGST 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 196 GDPKGVVLHHRGAYLNACA--GALIFQLGPRSIYLWtLPMFHCNGWSHTWAVTLSGGTHVClrKVQPdaihaaiaehavT 273
Cdd:PRK07768 165 GSPKAVQITHGNLYANAEAmfVAAEFDVETDVMVSW-LPLFHDMGMVGFLTVPMYFGAELV--KVTP------------M 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 274 HLSAAPVVMSMLIHAEHASVPAVP------------------------VSVITGGAAP--PSAVIAAMEA------RGFS 321
Cdd:PRK07768 230 DFLRDPLLWAELISKYRGTMTAAPnfayallarrlrrqakpgafdlssLRFALNGAEPidPADVEDLLDAgarfglRPEA 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 322 ITHAYGMTE-----SYGPSTLCLWQPGVDELPLEARAQFM-SRQGVAH------PMLE--EATVLDTDtGRPVPAdgRTL 387
Cdd:PRK07768 310 ILPAYGMAEatlavSFSPCGAGLVVDEVDADLLAALRRAVpATKGNTRrlatlgPPLPglEVRVVDED-GQVLPP--RGV 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 388 GELVVRGNTVMKGYLhNPEATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENIssleieevlyqHPEVVEA 466
Cdd:PRK07768 387 GVIELRGESVTPGYL-TMDGFIPAQdADGWLDTGDLGYLTEEGEVVVCGRVKDVIIMAGRNI-----------YPTDIER 454
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 467 AVVARPDSRWGETphafVALRADALPRTTG------------DDLVRWCRERLAH--FKA----PRHVTLTE---LPKTA 525
Cdd:PRK07768 455 AAARVEGVRPGNA----VAVRLDAGHSREGfavavesnafedPAEVRRIRHQVAHevVAEvgvrPRNVVVLGpgsIPKTP 530
|
....*.
gi 489250734 526 TGKIQK 531
Cdd:PRK07768 531 SGKLRR 536
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
452-528 |
4.16e-22 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 90.30 E-value: 4.16e-22
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489250734 452 EIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADalPRTTGDDLVRWCRERLAHFKAPRHVTLT-ELPKTATGK 528
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPG--VELLEEELVAHVREELGPYAVPKEVVFVdELPKTRSGK 76
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
47-456 |
6.86e-22 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 99.79 E-value: 6.86e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 47 SYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDRE 126
Cdd:PLN02736 80 TYGEAGTARTAIGSGLVQHGIPKGACVGLYFINRPEWLIVDHACSAYSYVSVPLYDTLGPDAVKFIVNHAEVAAIFCVPQ 159
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 127 FGAVAQQALAMLDAPPLLVGIDDDQAERADLAHDLD-----YEAFLAQG--DPgRPLSAPQNEwqSIA-INYTSGTTGDP 198
Cdd:PLN02736 160 TLNTLLSCLSEIPSVRLIVVVGGADEPLPSLPSGTGveivtYSKLLAQGrsSP-QPFRPPKPE--DVAtICYTSGTTGTP 236
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 199 KGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGgthVCLRKVQPDAIHAAIAEHAV--THLS 276
Cdd:PLN02736 237 KGVVLTHGNLIANVAGSSLSTKFYPSDVHISYLPLAHIYERVNQIVMLHYG---VAVGFYQGDNLKLMDDLAALrpTIFC 313
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 277 AAP------------------VVMSMLIHAEHAS--------VPAVP-----------------VSVITGGAAPPSAVIa 313
Cdd:PLN02736 314 SVPrlynriydgitnavkesgGLKERLFNAAYNAkkqalengKNPSPmwdrlvfnkikaklggrVRFMSSGASPLSPDV- 392
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 314 aME----ARGFSITHAYGMTEsygpsTLCLWQpGVDElplearAQFMSRQ-GVAHPMLEEATVlD------TDTGRPVPA 382
Cdd:PLN02736 393 -MEflriCFGGRVLEGYGMTE-----TSCVIS-GMDE------GDNLSGHvGSPNPACEVKLV-DvpemnyTSEDQPYPR 458
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 489250734 383 dgrtlGELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGDLAVLHPDGYVEIKDRAKDII-ISGGENISSLEIEEV 456
Cdd:PLN02736 459 -----GEICVRGPIIFKGYYKDEVQTREVIdEDGWLHTGDIGLWLPGGRLKIIDRKKNIFkLAQGEYIAPEKIENV 529
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
24-469 |
1.43e-20 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 95.35 E-value: 1.43e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 24 SFLKRAAQVYPQRDAVV-------YGARRY---SYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGA 93
Cdd:PRK09274 10 RHLPRAAQERPDQLAVAvpggrgaDGKLAYdelSFAELDARSDAIAHGLNAAGIGRGMRAVLMVTPSLEFFALTFALFKA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 94 GAVLVCI-------NIR---LEARSIAFI---LRHCAARVLicDREFGAVAQqaLAMLDAPPLLVGIDDDQAERADLAHD 160
Cdd:PRK09274 90 GAVPVLVdpgmgikNLKqclAEAQPDAFIgipKAHLARRLF--GWGKPSVRR--LVTVGGRLLWGGTTLATLLRDGAAAP 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 161 LDyeafLAQGDPGRPLsapqnewqsiAINYTSGTTGDPKGVVLHHRgaYLNACAGAL--IFQLGPRSIYLWTLPMFHCNG 238
Cdd:PRK09274 166 FP----MADLAPDDMA----------AILFTSGSTGTPKGVVYTHG--MFEAQIEALreDYGIEPGEIDLPTFPLFALFG 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 239 wshtwavTLSGGTHV-------CLRKVQPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPV--SVITGGAAPPS 309
Cdd:PRK09274 230 -------PALGMTSVipdmdptRPATVDPAKLFAAIERYGVTNLFGSPALLERLGRYGEANGIKLPSlrRVISAGAPVPI 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 310 AVIA---AMEARGFSITHAYGMTESYGPSTLclwqpGVDELPLEARAqfMSRQG----VAHPM---------LEEATVLD 373
Cdd:PRK09274 303 AVIErfrAMLPPDAEILTPYGATEALPISSI-----ESREILFATRA--ATDNGagicVGRPVdgvevriiaISDAPIPE 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 374 TDTGRPVPADGrtLGELVVRGNTVMKGYLHNPEATRAA-LADG----WLHTGDLAVLHPDGYVEIKDRAKDIIISGGENI 448
Cdd:PRK09274 376 WDDALRLATGE--IGEIVVAGPMVTRSYYNRPEATRLAkIPDGqgdvWHRMGDLGYLDAQGRLWFCGRKAHRVETAGGTL 453
|
490 500
....*....|....*....|.
gi 489250734 449 SSLEIEEVLYQHPEVVEAAVV 469
Cdd:PRK09274 454 YTIPCERIFNTHPGVKRSALV 474
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
47-546 |
3.34e-20 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 94.58 E-value: 3.34e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 47 SYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLI-CDr 125
Cdd:PLN02654 122 TYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAESLAQRIVDCKPKVVItCN- 200
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 126 efgAVAQQALAMLDAPPLLVGIDDDQAERADLAHDLDYEAFLAQGDPGRPLSAPQNEW-QSIAIN--------------- 189
Cdd:PLN02654 201 ---AVKRGPKTINLKDIVDAALDESAKNGVSVGICLTYENQLAMKREDTKWQEGRDVWwQDVVPNyptkcevewvdaedp 277
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 190 ----YTSGTTGDPKGVvLHHRGAYL--NACAGALIFQLGPRSIYLWTLPMFHCNGWSH-TWAVTLSGGTHVCLRKVQ--P 260
Cdd:PLN02654 278 lfllYTSGSTGKPKGV-LHTTGGYMvyTATTFKYAFDYKPTDVYWCTADCGWITGHSYvTYGPMLNGATVLVFEGAPnyP 356
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 261 DAIH--AAIAEHAVTHLSAAPVVMSMLI-----HAEHASVPAVPVSVITGGAAPPSAVIAAMEARGFS---ITHAYGMTE 330
Cdd:PLN02654 357 DSGRcwDIVDKYKVTIFYTAPTLVRSLMrdgdeYVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSrcpISDTWWQTE 436
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 331 SYGPSTLCLwqPGV-DELPLEARAQFMSRQGVahpmleeatVLDtDTGRPVpaDGRTLGELVVRGN-----TVMKGYLHN 404
Cdd:PLN02654 437 TGGFMITPL--PGAwPQKPGSATFPFFGVQPV---------IVD-EKGKEI--EGECSGYLCVKKSwpgafRTLYGDHER 502
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 405 PEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFV 484
Cdd:PLN02654 503 YETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFV 582
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 489250734 485 ALrADALPRTTG--DDLVRWCRERLAHFKAPRHVTLTE-LPKTATGKIQKFVLREWA-RQEEEAGD 546
Cdd:PLN02654 583 TL-VEGVPYSEElrKSLILTVRNQIGAFAAPDKIHWAPgLPKTRSGKIMRRILRKIAsRQLDELGD 647
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
37-464 |
5.78e-20 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 93.89 E-value: 5.78e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 37 DAVVYGARRY-SYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRH 115
Cdd:PTZ00216 112 EVTHFNETRYiTYAELWERIVNFGRGLAELGLTKGSNVAIYEETRWEWLASIYGIWSQSMVAATVYANLGEDALAYALRE 191
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 116 CAARVLICDrefGAVAQQALAMLD---APPLLVGIDDDQAERADlAHDLDYEAF---LAQGDPG---RPLSAPQNEWQSI 186
Cdd:PTZ00216 192 TECKAIVCN---GKNVPNLLRLMKsggMPNTTIIYLDSLPASVD-TEGCRLVAWtdvVAKGHSAgshHPLNIPENNDDLA 267
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 187 AINYTSGTTGDPKGVVLHHRGAY--LNACAGALIFQLGPRS---IYLWTLPMFHCNGWSHTwAVTLSGGTHVC------- 254
Cdd:PTZ00216 268 LIMYTSGTTGDPKGVMHTHGSLTagILALEDRLNDLIGPPEedeTYCSYLPLAHIMEFGVT-NIFLARGALIGfgsprtl 346
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 255 ----------LRKVQPdaihaaiaehavTHLSAAPVVMSMLIHAEHASVPAV-------------------------P-- 297
Cdd:PTZ00216 347 tdtfarphgdLTEFRP------------VFLIGVPRIFDTIKKAVEAKLPPVgslkrrvfdhayqsrlralkegkdtPyw 414
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 298 ---------------VSVITGGAAPPSA-------VIAAMEARGfsithaYGMTEsygpsTLCLwqpGVDELP--LEAra 353
Cdd:PTZ00216 415 nekvfsapravlggrVRAMLSGGGPLSAatqefvnVVFGMVIQG------WGLTE-----TVCC---GGIQRTgdLEP-- 478
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 354 qfmsrqGVAHPML--EEATVLDTD----TGRPVPAdgrtlGELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGDLAVLH 426
Cdd:PTZ00216 479 ------NAVGQLLkgVEMKLLDTEeykhTDTPEPR-----GEILLRGPFLFKGYYKQEELTREVLdEDGWFHTGDVGSIA 547
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 489250734 427 PDGYVEIKDR----AKDIIisgGENIsSLEIEEVLYQHPEVV 464
Cdd:PTZ00216 548 ANGTLRIIGRvkalAKNCL---GEYI-ALEALEALYGQNELV 585
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
34-529 |
6.22e-20 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 92.50 E-value: 6.22e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 34 PQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFIL 113
Cdd:cd17644 14 PDAVAVVFEDQQLTYEELNTKANQLAHYLQSLGVKSESLVGICVERSLEMIIGLLAILKAGGAYVPLDPNYPQERLTYIL 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 114 RHCAARVLicdrefgaVAQQalamldappllvgidddqaeradlaHDLDYeaflaqgdpgrplsapqnewqsiaINYTSG 193
Cdd:cd17644 94 EDAQISVL--------LTQP-------------------------ENLAY------------------------VIYTSG 116
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 194 TTGDPKGVVLHHRgAYLNacagaliFQLGPRSIYLWTLPMFHCNGWSHTWAVT--------LSGGTHVcLR--KVQPDAI 263
Cdd:cd17644 117 STGKPKGVMIEHQ-SLVN-------LSHGLIKEYGITSSDRVLQFASIAFDVAaeeiyvtlLSGATLV-LRpeEMRSSLE 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 264 HAAI--AEHAVTHLSAAPVVMSMLIHA---EHASVPAVPVSVITGG-AAPPSAVIAAMEARGFSIT--HAYGMTESYGPS 335
Cdd:cd17644 188 DFVQyiQQWQLTVLSLPPAYWHLLVLElllSTIDLPSSLRLVIVGGeAVQPELVRQWQKNVGNFIQliNVYGPTEATIAA 267
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 336 TLCLwqpgvdelPLEARAQFMSRQGVAHPMLEEAT-VLDtDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAALAD 414
Cdd:cd17644 268 TVCR--------LTQLTERNITSVPIGRPIANTQVyILD-ENLQPVPVG--VPGELHIGGVGLARGYLNRPELTAEKFIS 336
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 415 GWLH---------TGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVA 485
Cdd:cd17644 337 HPFNsseserlykTGDLARYLPDGNIEYLGRIDNQVKIRGFRIELGEIEAVLSQHNDVKTAVVIVREDQPGNKRLVAYIV 416
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 489250734 486 LRADALPRTTgdDLVRWCRERLAHFKAPRH-VTLTELPKTATGKI 529
Cdd:cd17644 417 PHYEESPSTV--ELRQFLKAKLPDYMIPSAfVVLEELPLTPNGKI 459
|
|
| PLN02614 |
PLN02614 |
long-chain acyl-CoA synthetase |
47-531 |
9.42e-20 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 166255 [Multi-domain] Cd Length: 666 Bit Score: 93.16 E-value: 9.42e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 47 SYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDR- 125
Cdd:PLN02614 81 TYQEVYDIVIKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEACNAHGLYCVPLYDTLGAGAVEFIISHSEVSIVFVEEk 160
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 126 ---EFGAVAQQALAMLDAPPLLVGIDDDQAERAD----LAHDLDyeAFLAQGDpGRPLSAPQNEWQSI-AINYTSGTTGD 197
Cdd:PLN02614 161 kisELFKTCPNSTEYMKTVVSFGGVSREQKEEAEtfglVIYAWD--EFLKLGE-GKQYDLPIKKKSDIcTIMYTSGTTGD 237
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 198 PKGVVLHHRgAYLNACAGALIF------QLGPRSIYLWTLPMFHC------------NGWSHTW---------------- 243
Cdd:PLN02614 238 PKGVMISNE-SIVTLIAGVIRLlksanaALTVKDVYLSYLPLAHIfdrvieecfiqhGAAIGFWrgdvklliedlgelkp 316
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 244 ----AV-------------TLSGGTHvcLRKVQPDAIHAAI---AEHAVTHLSAAPVVMSMLIHAEHASVPAvPVSVITG 303
Cdd:PLN02614 317 tifcAVprvldrvysglqkKLSDGGF--LKKFVFDSAFSYKfgnMKKGQSHVEASPLCDKLVFNKVKQGLGG-NVRIILS 393
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 304 GAAPPSAVIAAMeARGFSITH---AYGMTESYGPSTLCLwqpgVDELPLearaqfMSRQGVAHP----MLEEATVLDTDT 376
Cdd:PLN02614 394 GAAPLASHVESF-LRVVACCHvlqGYGLTESCAGTFVSL----PDELDM------LGTVGPPVPnvdiRLESVPEMEYDA 462
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 377 GRPVPAdgrtlGELVVRGNTVMKGYLHNPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDII-ISGGENISSLEIEE 455
Cdd:PLN02614 463 LASTPR-----GEICIRGKTLFSGYYKREDLTKEVLIDGWLHTGDVGEWQPNGSMKIIDRKKNIFkLSQGEYVAVENIEN 537
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 456 VlYQHPEVVEAAVVarpdsrWGETPHAFVALRADALPRTtgddLVRWCRER--------LAHFKAPRHVTLTELPKTATG 527
Cdd:PLN02614 538 I-YGEVQAVDSVWV------YGNSFESFLVAIANPNQQI----LERWAAENgvsgdynaLCQNEKAKEFILGELVKMAKE 606
|
....
gi 489250734 528 KIQK 531
Cdd:PLN02614 607 KKMK 610
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
30-534 |
9.92e-20 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 93.69 E-value: 9.92e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 30 AQV--YPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEAR 107
Cdd:PRK05691 3728 AQVaaHPQRIAASCLDQQWSYAELNRAANRLGHALRAAGVGVDQPVALLAERGLDLLGMIVGSFKAGAGYLPLDPGLPAQ 3807
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 108 SIAFILRHCAARVLICDRefgAVAQQALAMLDA------PPLLVGIDDDQAEradlahdldyeafLAQGDPGRpLSAPQN 181
Cdd:PRK05691 3808 RLQRIIELSRTPVLVCSA---ACREQARALLDElgcanrPRLLVWEEVQAGE-------------VASHNPGI-YSGPDN 3870
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 182 EWQSIainYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPM-FHCNGWSHTWAVTLSGGTHVCLRKV-- 258
Cdd:PRK05691 3871 LAYVI---YTSGSTGLPKGVMVEQRGMLNNQLSKVPYLALSEADVIAQTASQsFDISVWQFLAAPLFGARVEIVPNAIah 3947
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 259 QPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVSVITGGAAPPSaviaaMEARGFSITHAYGMTESYGPSTLC 338
Cdd:PRK05691 3948 DPQGLLAHVQAQGITVLESVPSLIQGMLAEDRQALDGLRWMLPTGEAMPPE-----LARQWLQRYPQIGLVNAYGPAECS 4022
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 339 lwqpgvDELPLeARAQFMSRQGVAHP-----------MLEEATVLdtdtgrpVPADGrtLGELVVRGNTVMKGYLHNPEA 407
Cdd:PRK05691 4023 ------DDVAF-FRVDLASTRGSYLPigsptdnnrlyLLDEALEL-------VPLGA--VGELCVAGTGVGRGYVGDPLR 4086
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 408 TR--------AALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGET 479
Cdd:PRK05691 4087 TAlafvphpfGAPGERLYRTGDLARRRSDGVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVREAAVAVQEGVNGKHL 4166
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 489250734 480 PHAFVALRADALPRTTGDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQKFVL 534
Cdd:PRK05691 4167 VGYLVPHQTVLAQGALLERIKQRLRAELPDYMVPLHwLWLDRLPLNANGKLDRKAL 4222
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
177-543 |
2.25e-19 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 90.82 E-value: 2.25e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 177 SAPQNEWQSIAINyTSGTTGDPKGVVlhHRGAYLNACAGALI--FQLGPRSIYLWtLPMFHCNGWSHTWAVTLSGGTHVC 254
Cdd:PRK07445 115 ILPNLETGWIMIP-TGGSSGQIRFAI--HTWETLTASVQGFQryFQLQQVNSFCV-LPLYHVSGLMQFMRSFLTGGKLVI 190
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 255 L--RKVQPDAIHAAIAEHAVthLSAAPVVMSMLIHaEHASVPAVPVSVITGGAAPPSAVIAamEARGFSITHA--YGMTE 330
Cdd:PRK07445 191 LpyKRLKSGQELPPNPSDFF--LSLVPTQLQRLLQ-LRPQWLAQFRTILLGGAPAWPSLLE--QARQLQLRLAptYGMTE 265
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 331 SygPSTLCLWQPgvdelplearAQFMsrQGvahpmleeatvlDTDTGRPVPADGRTL-----GELVVRGNTVMKGYLHNP 405
Cdd:PRK07445 266 T--ASQIATLKP----------DDFL--AG------------NNSSGQVLPHAQITIpanqtGNITIQAQSLALGYYPQI 319
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 406 EAtraalADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGEtphAFVA 485
Cdd:PRK07445 320 LD-----SQGIFETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGE---VVTA 391
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*....
gi 489250734 486 LRADALPRTTGDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQKFVLREWARQEEE 543
Cdd:PRK07445 392 IYVPKDPSISLEELKTAIKDQLSPFKQPKHwIPVPQLPRNPQGKINRQQLQQIAVQRLG 450
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
191-529 |
1.54e-18 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 87.41 E-value: 1.54e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 191 TSGTTGDPKGVVLhhRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQ-------PDAI 263
Cdd:PRK07824 43 TSGTTGTPKGAML--TAAALTASADATHDRLGGPGQWLLALPAHHIAGLQVLVRSVIAGSEPVELDVSAgfdptalPRAV 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 264 HAAIAEHAVTHLSAAPVVMSMlIHAEHASVPAVPVSVITGGAAPPSAVIAAMEARGFSITHAYGMTESYGpstlclwqpg 343
Cdd:PRK07824 121 AELGGGRRYTSLVPMQLAKAL-DDPAATAALAELDAVLVGGGPAPAPVLDAAAAAGINVVRTYGMSETSG---------- 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 344 vdelplearaqfmsrqgvahpmleeATVLDtdtGRPVP------ADGRT-LGelvvrGNTVMKGYlHNPEATRAALADGW 416
Cdd:PRK07824 190 -------------------------GCVYD---GVPLDgvrvrvEDGRIaLG-----GPTLAKGY-RNPVDPDPFAEPGW 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 417 LHTGDLAVLHpDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETPHAfvALRADALPRTTG 496
Cdd:PRK07824 236 FRTDDLGALD-DGVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQRVVA--AVVGDGGPAPTL 312
|
330 340 350
....*....|....*....|....*....|....
gi 489250734 497 DDLVRWCRERLAHFKAPRHV-TLTELPKTATGKI 529
Cdd:PRK07824 313 EALRAHVARTLDRTAAPRELhVVDELPRRGIGKV 346
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
34-530 |
2.02e-18 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 88.87 E-value: 2.02e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 34 PQRDAVVYGA-----RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARS 108
Cdd:cd05943 82 ADDPAAIYAAedgerTEVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSPDFGVPG 161
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 109 IAFILRHCAARVLI-CDR-EFGA--------VAQQALAMLDAPPLLVGIDDDQAERADLAHD---LDYEAFLAQGDPGRP 175
Cdd:cd05943 162 VLDRFGQIEPKVLFaVDAyTYNGkrhdvrekVAELVKGLPSLLAVVVVPYTVAAGQPDLSKIakaLTLEDFLATGAAGEL 241
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 176 LSAPQNEWQSIAINYTSGTTGDPKGVVLHHRGAYLNACAgALIFQ--LGPRSIYLWtlpmFHCNGWSH-TWAVT-LSGGT 251
Cdd:cd05943 242 EFEPLPFDHPLYILYSSGTTGLPKCIVHGAGGTLLQHLK-EHILHcdLRPGDRLFY----YTTCGWMMwNWLVSgLAVGA 316
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 252 HVCLRKVQP-----DAIHAAIAEHAVTHLSAAPVVMSMLI------HAEHaSVPAVPVSVITGGAAPPSAVIAAMEARGF 320
Cdd:cd05943 317 TIVLYDGSPfypdtNALWDLADEEGITVFGTSAKYLDALEkaglkpAETH-DLSSLRTILSTGSPLKPESFDYVYDHIKP 395
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 321 SIthaygMTESYGPST-----LCLWQPGVDELPLEARAqfmsrqgvahPMLEEATVLDTDTGRPVPADgrtLGELV-VRG 394
Cdd:cd05943 396 DV-----LLASISGGTdiiscFVGGNPLLPVYRGEIQC----------RGLGMAVEAFDEEGKPVWGE---KGELVcTKP 457
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 395 NTVMKGYLHNPEAT---RAALADG----WLHtGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAA 467
Cdd:cd05943 458 FPSMPVGFWNDPDGsryRAAYFAKypgvWAH-GDWIEITPRGGVVILGRSDGTLNPGGVRIGTAEIYRVVEKIPEVEDSL 536
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489250734 468 VVARPDSRWGETPHAFVALRADAlprTTGDDLVRWCRERLAHFKAPRHV-----TLTELPKTATGKIQ 530
Cdd:cd05943 537 VVGQEWKDGDERVILFVKLREGV---ELDDELRKRIRSTIRSALSPRHVpakiiAVPDIPRTLSGKKV 601
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
44-529 |
2.93e-18 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 88.08 E-value: 2.93e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 44 RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEmleAHYgvpgagAVLVCIniRLEA-RSI---AFILRHCAAR 119
Cdd:PRK10524 83 RTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAE---AAF------AMLACA--RIGAiHSVvfgGFASHSLAAR 151
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 120 ------VLICDREFGAVA----------QQALAMLDAPP---LLV--GIDDDQAERAdlaHDLDYEAFLAQ-GDPGRPLs 177
Cdd:PRK10524 152 iddakpVLIVSADAGSRGgkvvpykpllDEAIALAQHKPrhvLLVdrGLAPMARVAG---RDVDYATLRAQhLGARVPV- 227
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 178 apqnEW----QSIAINYTSGTTGDPKGVvlhHR--GAYLNACAGAL--IFQLGPRSIYLWTLPMfhcnGW--SHTWAV-- 245
Cdd:PRK10524 228 ----EWlesnEPSYILYTSGTTGKPKGV---QRdtGGYAVALATSMdtIFGGKAGETFFCASDI----GWvvGHSYIVya 296
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 246 -TLSGGTHVCLR--KVQPDAIH--AAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVS----VITGGA---APPSAVIa 313
Cdd:PRK10524 297 pLLAGMATIMYEglPTRPDAGIwwRIVEKYKVNRMFSAPTAIRVLKKQDPALLRKHDLSslraLFLAGEpldEPTASWI- 375
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 314 aMEARGFSITHAYGMTESYGPsTLCLwQPGVDELPlearaqfmSRQGVA-HPMLE-EATVLDTDTGRPVPADGRtlGELV 391
Cdd:PRK10524 376 -SEALGVPVIDNYWQTETGWP-ILAI-ARGVEDRP--------TRLGSPgVPMYGyNVKLLNEVTGEPCGPNEK--GVLV 442
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 392 VRG-------NTV-------MKGYlhnpeatraaladgWLH-------TGDLAVLHPDGYVEIKDRAKDIIISGGENISS 450
Cdd:PRK10524 443 IEGplppgcmQTVwgdddrfVKTY--------------WSLfgrqvysTFDWGIRDADGYYFILGRTDDVINVAGHRLGT 508
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 451 LEIEEVLYQHPEVVEAAVVARPDSRWGETPHAFVALRADALPRTTGD------DLVRWCRERLAHFKAPRHVTL-TELPK 523
Cdd:PRK10524 509 REIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVPKDSDSLADREArlalekEIMALVDSQLGAVARPARVWFvSALPK 588
|
....*.
gi 489250734 524 TATGKI 529
Cdd:PRK10524 589 TRSGKL 594
|
|
| PLN02861 |
PLN02861 |
long-chain-fatty-acid-CoA ligase |
20-463 |
2.95e-17 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178452 [Multi-domain] Cd Length: 660 Bit Score: 85.28 E-value: 2.95e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 20 LSPLSFLKRAAQVYP------QRDAVVYGARRY---SYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGV 90
Cdd:PLN02861 43 DSPWQFFSDAVKKYPnnqmlgRRQVTDSKVGPYvwlTYKEVYDAAIRIGSAIRSRGVNPGDRCGIYGSNCPEWIIAMEAC 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 91 PGAGAVLVCINIRLEARSIAFILRHCAARVLICDRE----FGAVAQQALAMLDAPPLLVGIDDDQAERADL--AHDLDYE 164
Cdd:PLN02861 123 NSQGITYVPLYDTLGANAVEFIINHAEVSIAFVQESkissILSCLPKCSSNLKTIVSFGDVSSEQKEEAEElgVSCFSWE 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 165 AFLAQGDPGRPLSAPQNEwQSIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSI-----YLWTLPMFHCNG- 238
Cdd:PLN02861 203 EFSLMGSLDCELPPKQKT-DICTIMYTSGTTGEPKGVILTNRAIIAEVLSTDHLLKVTDRVAteedsYFSYLPLAHVYDq 281
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 239 ---------------------------------------------WSHTWAVTLSGGThvcLRKVQPDAIHAAIAEHAVT 273
Cdd:PLN02861 282 vietyciskgasigfwqgdirylmedvqalkptifcgvprvydriYTGIMQKISSGGM---LRKKLFDFAYNYKLGNLRK 358
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 274 HLS---AAPVvMSMLIHAEHASVPAVPVSVITGGAAP-PSAVIAAMEARGFS-ITHAYGMTESYGPSTLCLwqpgVDELP 348
Cdd:PLN02861 359 GLKqeeASPR-LDRLVFDKIKEGLGGRVRLLLSGAAPlPRHVEEFLRVTSCSvLSQGYGLTESCGGCFTSI----ANVFS 433
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 349 LearaqfMSRQGVAHPM----LEEATVLDTDTGRPVPAdgrtlGELVVRGNTVMKGYLHNPEATRAALADGWLHTGDLAV 424
Cdd:PLN02861 434 M------VGTVGVPMTTiearLESVPEMGYDALSDVPR-----GEICLRGNTLFSGYHKRQDLTEEVLIDGWFHTGDIGE 502
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 489250734 425 LHPDGYVEIKDRAKDII-ISGGENISSLEIEEVLYQHPEV 463
Cdd:PLN02861 503 WQPNGAMKIIDRKKNIFkLSQGEYVAVENLENTYSRCPLI 542
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
41-238 |
5.19e-17 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 83.88 E-value: 5.19e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 41 YGARRYSYRQLHERSCALASALER-VGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAAR 119
Cdd:cd05938 1 FEGETYTYRDVDRRSNQAARALLAhAGLRPGDTVALLLGNEPAFLWIWLGLAKLGCPVAFLNTNIRSKSLLHCFRCCGAK 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 120 VLICDREFGAVAQQALAMLDAPPLLVGIDDDQAERADLahdldyEAFLAQGD--PGRP----LSAPQNeWQSIAIN-YTS 192
Cdd:cd05938 81 VLVVAPELQEAVEEVLPALRADGVSVWYLSHTSNTEGV------ISLLDKVDaaSDEPvpasLRAHVT-IKSPALYiYTS 153
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|
gi 489250734 193 GTTGDPKGVVLHHrgayLNACAGALIFQL-GPRS---IYLwTLPMFHCNG 238
Cdd:cd05938 154 GTTGLPKAARISH----LRVLQCSGFLSLcGVTAddvIYI-TLPLYHSSG 198
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
47-472 |
5.23e-17 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 84.40 E-value: 5.23e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 47 SYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICD-- 124
Cdd:PLN02387 108 TYGQVFERVCNFASGLVALGHNKEERVAIFADTRAEWLIALQGCFRQNITVVTIYASLGEEALCHSLNETEVTTVICDsk 187
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 125 --REFGAVAQQalamLDAPPLLVGIDDDQAERADLAHDLDY---EAF-----LAQGDPGRP-LSAPQNewqsIA-INYTS 192
Cdd:PLN02387 188 qlKKLIDISSQ----LETVKRVIYMDDEGVDSDSSLSGSSNwtvSSFsevekLGKENPVDPdLPSPND----IAvIMYTS 259
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 193 GTTGDPKGVVLHHrGAYLNACAG--ALIFQLGPRSIYLWTLPMFH-----------CNGWS--HTWAVTLSGGTHVCLRK 257
Cdd:PLN02387 260 GSTGLPKGVMMTH-GNIVATVAGvmTVVPKLGKNDVYLAYLPLAHilelaaesvmaAVGAAigYGSPLTLTDTSNKIKKG 338
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 258 VQPDaihaaiaehaVTHLSaaPVVMsmlihaehASVPAV-----------------------------PVSVITG---GA 305
Cdd:PLN02387 339 TKGD----------ASALK--PTLM--------TAVPAIldrvrdgvrkkvdakgglakklfdiaykrRLAAIEGswfGA 398
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 306 APPSAVI----------AAMEAR-----------------------GFSITHAYGMTESYGPSTLCLWQ-PGVdelplea 351
Cdd:PLN02387 399 WGLEKLLwdalvfkkirAVLGGRirfmlsggaplsgdtqrfiniclGAPIGQGYGLTETCAGATFSEWDdTSV------- 471
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 352 raqfmSRQG--VAHPML-----EEATVLDTDtgRPVPAdgrtlGELVVRGNTVMKGYLHNPEATRAALADG-----WLHT 419
Cdd:PLN02387 472 -----GRVGppLPCCYVklvswEEGGYLISD--KPMPR-----GEIVIGGPSVTLGYFKNQEKTDEVYKVDergmrWFYT 539
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 489250734 420 GDLAVLHPDGYVEIKDRAKDII-ISGGENISSLEIEEVLYQHPEVVEAAVVARP 472
Cdd:PLN02387 540 GDIGQFHPDGCLEIIDRKKDIVkLQHGEYVSLGKVEAALSVSPYVDNIMVHADP 593
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
188-536 |
1.72e-14 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 75.99 E-value: 1.72e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 188 INYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGT-----HVCLRKVQPDA 262
Cdd:cd05908 111 IQFSSGSTGDPKGVMLTHENLVHNMFAILNSTEWKTKDRILSWMPLTHDMGLIAFHLAPLIAGMnqylmPTRLFIRRPIL 190
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 263 IHAAIAEHAVTHLS----AAPVVMSMLIHAEHASVPAVPVSVITGGAAPPSA-----VIAAMEARGF---SITHAYGMTE 330
Cdd:cd05908 191 WLKKASEHKATIVSspnfGYKYFLKTLKPEKANDWDLSSIRMILNGAEPIDYelcheFLDHMSKYGLkrnAILPVYGLAE 270
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 331 -SYGPSTLCLWQP------GVDELPLEARAQFMSRQG--------VAHPmLEEATVLDTDTGRPVPADGrTLGELVVRGN 395
Cdd:cd05908 271 aSVGASLPKAQSPfktitlGRRHVTHGEPEPEVDKKDsecltfveVGKP-IDETDIRICDEDNKILPDG-YIGHIQIRGK 348
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 396 TVMKGYLHNPEATRAAL-ADGWLHTGDLAVLHpDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVA---- 470
Cdd:cd05908 349 NVTPGYYNNPEATAKVFtDDGWLKTGDLGFIR-NGRLVITGREKDIIFVNGQNVYPHDIERIAEELEGVELGRVVAcgvn 427
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 489250734 471 RPDSRwGETPHAFValradaLPRTTGDDLVRWCRERLAHFKAPRHVTLTE------LPKTATGKIQKFVLRE 536
Cdd:cd05908 428 NSNTR-NEEIFCFI------EHRKSEDDFYPLGKKIKKHLNKRGGWQINEvlpirrIPKTTSGKVKRYELAQ 492
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
25-544 |
1.99e-14 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 76.75 E-value: 1.99e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 25 FLKRAAQVyPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRL 104
Cdd:PRK05691 2194 FAAQAART-PQAPALTFAGQTLSYAELDARANRLARALRERGVGPQVRVGLALERSLEMVVGLLAILKAGGAYVPLDPEY 2272
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 105 EARSIAFILRHCAARVLICDREFGAvaqqALAMLDAPPLLVGIDDDQAERADLAhdldyeaflaqGDPGRPLSAPQNEWQ 184
Cdd:PRK05691 2273 PLERLHYMIEDSGIGLLLSDRALFE----ALGELPAGVARWCLEDDAAALAAYS-----------DAPLPFLSLPQHQAY 2337
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 185 SIainYTSGTTGDPKGVVLHHrGAYLNACAgALIFQLGPRS----IYLWTLpmfHCNGWSHTWAVTLSGGTHVCLR-KVQ 259
Cdd:PRK05691 2338 LI---YTSGSTGKPKGVVVSH-GEIAMHCQ-AVIERFGMRAddceLHFYSI---NFDAASERLLVPLLCGARVVLRaQGQ 2409
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 260 PDAIH--AAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVS-VITGGAAPPSAVIAAMEA--RGFSITHAYGMTESYgp 334
Cdd:PRK05691 2410 WGAEEicQLIREQQVSILGFTPSYGSQLAQWLAGQGEQLPVRmCITGGEALTGEHLQRIRQafAPQLFFNAYGPTETV-- 2487
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 335 stlclwqpgvdELPLEARAQFMSRQGVAH-PM-----LEEATVLDTDTGrPVPADGrtLGELVVRGNTVMKGYLHNPEAT 408
Cdd:PRK05691 2488 -----------VMPLACLAPEQLEEGAASvPIgrvvgARVAYILDADLA-LVPQGA--TGELYVGGAGLAQGYHDRPGLT 2553
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 409 RAA-LADGWLH-------TGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARpDSRWGETP 480
Cdd:PRK05691 2554 AERfVADPFAAdggrlyrTGDLVRLRADGLVEYVGRIDHQVKIRGFRIELGEIESRLLEHPAVREAVVLAL-DTPSGKQL 2632
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 489250734 481 HAFVALRADALPRTTGDDLvrwcRERL-AHFKA-------PRH-VTLTELPKTATGKIQKFVLR----EWARQEEEA 544
Cdd:PRK05691 2633 AGYLVSAVAGQDDEAQAAL----REALkAHLKQqlpdymvPAHlILLDSLPLTANGKLDRRALPapdpELNRQAYQA 2705
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
44-469 |
2.05e-14 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 75.57 E-value: 2.05e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 44 RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSiafiLRHCAARVlic 123
Cdd:cd05910 1 SRLSFRELDERSDRIAQGLTAYGIRRGMRAVLMVPPGPDFFALTFALFKAGAVPVLIDPGMGRKN----LKQCLQEA--- 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 124 drefgavaqqalamldAPPLLVGIdddqaeradlahdldyeaFLAqGDPGrplsapqnewqsiAINYTSGTTGDPKGVVL 203
Cdd:cd05910 74 ----------------EPDAFIGI------------------PKA-DEPA-------------AILFTSGSTGTPKGVVY 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 204 HHRgaYLNACAGALIFQLGPRS--IYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPDAIHAAIAEHAVTHLSAAPVV 281
Cdd:cd05910 106 RHG--TFAAQIDALRQLYGIRPgeVDLATFPLFALFGPALGLTSVIPDMDPTRPARADPQKLVGAIRQYGVSIVFGSPAL 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 282 MSMLihAEHASVPAVPVS----VITGGAAPPSAVIAA---MEARGFSITHAYGMTESYGPSTLclwqpGVDELpLEARAQ 354
Cdd:cd05910 184 LERV--ARYCAQHGITLPslrrVLSAGAPVPIALAARlrkMLSDEAEILTPYGATEALPVSSI-----GSREL-LATTTA 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 355 FMSRQG---VAHPM--LEEATVLDTDTGRPVPADGRTL-----GELVVRGNTVMKGYLHNPEATRAA----LADGWLH-T 419
Cdd:cd05910 256 ATSGGAgtcVGRPIpgVRVRIIEIDDEPIAEWDDTLELprgeiGEITVTGPTVTPTYVNRPVATALAkiddNSEGFWHrM 335
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|
gi 489250734 420 GDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVV 469
Cdd:cd05910 336 GDLGYLDDEGRLWFCGRKAHRVITTGGTLYTEPVERVFNTHPGVRRSALV 385
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
47-494 |
2.65e-14 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 75.57 E-value: 2.65e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 47 SYRQLHERSCALASALE-RVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDR 125
Cdd:cd17632 69 TYAELWERVGAVAAAHDpEQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAPILAETEPRLLAVSA 148
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 126 EFGAVAQQALAMLDAPPLLVGID---DDQAERADLA-------------HDLDYEAFLAQGDPGRPLSAPQNEWQSIA-I 188
Cdd:cd17632 149 EHLDLAVEAVLEGGTPPRLVVFDhrpEVDAHRAALEsarerlaavgipvTTLTLIAVRGRDLPPAPLFRPEPDDDPLAlL 228
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 189 NYTSGTTGDPKGVVLHHR---GAYLNACAGALIFQlgPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKvqPDAIHA 265
Cdd:cd17632 229 IYTSGSTGTPKGAMYTERlvaTFWLKVSSIQDIRP--PASITLNFMPMSHIAGRISLYGTLARGGTAYFAAA--SDMSTL 304
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 266 AIAEHAV--THLSAAPVVMSMLiHAEHASVpaVPVSVITG----------------------------GAAPPSAVIAA- 314
Cdd:cd17632 305 FDDLALVrpTELFLVPRVCDML-FQRYQAE--LDRRSVAGadaetlaervkaelrervlggrllaavcGSAPLSAEMKAf 381
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 315 MEA-RGFSITHAYGMTESyGPSTL--CLWQPGVDELPLearaqfmsrqgVAHPmleEATVLDTDtgRPVPAdgrtlGELV 391
Cdd:cd17632 382 MESlLDLDLHDGYGSTEA-GAVILdgVIVRPPVLDYKL-----------VDVP---ELGYFRTD--RPHPR-----GELL 439
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 392 VRGNTVMKGYLHNPEATRAAL-ADGWLHTGD-LAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVE---- 465
Cdd:cd17632 440 VKTDTLFPGYYKRPEVTAEVFdEDGFYRTGDvMAELGPDRLVYVDRRNNVLKLSQGEFVTVARLEAVFAASPLVRQifvy 519
|
490 500 510
....*....|....*....|....*....|....*...
gi 489250734 466 ---------AAVVARPDSRWGETPHAFVALRADALPRT 494
Cdd:cd17632 520 gnserayllAVVVPTQDALAGEDTARLRAALAESLQRI 557
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
30-469 |
4.81e-14 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 74.55 E-value: 4.81e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 30 AQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSI 109
Cdd:PRK04813 12 AQTQPDFPAYDYLGEKLTYGQLKEDSDALAAFIDSLKLPDKSPIIVFGHMSPEMLATFLGAVKAGHAYIPVDVSSPAERI 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 110 AFILRHCAARVLICDREFgavaqqalamldaPPLLVGIDDDQAEraDLAhdldyEAFLAQGDPgrPLSAPQNEWQSIAIN 189
Cdd:PRK04813 92 EMIIEVAKPSLIIATEEL-------------PLEILGIPVITLD--ELK-----DIFATGNPY--DFDHAVKGDDNYYII 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 190 YTSGTTGDPKGVVLHHrgAYLNACAGALI--FQLGPRSIYLWTLP-------MfhcngwshTWAVTL-SGGTHVCLRKVq 259
Cdd:PRK04813 150 FTSGTTGKPKGVQISH--DNLVSFTNWMLedFALPEGPQFLNQAPysfdlsvM--------DLYPTLaSGGTLVALPKD- 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 260 pdaihaaiaehavthLSAAPVVMsmlihaeHASVPAVPVSVITggaAPPSAVIAAMEARGF------SITH--------- 324
Cdd:PRK04813 219 ---------------MTANFKQL-------FETLPQLPINVWV---STPSFADMCLLDPSFneehlpNLTHflfcgeelp 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 325 ------------------AYGMTESygpsTLCLWQPGVDE--------LPLearaqfmsrqGVAHPMLEeaTVLDTDTGR 378
Cdd:PRK04813 274 hktakkllerfpsatiynTYGPTEA----TVAVTSIEITDemldqykrLPI----------GYAKPDSP--LLIIDEEGT 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 379 PVPADGRtlGELVVRGNTVMKGYLHNPEATRAAL--ADGW--LHTGDLAVLhPDGYVEIKDRAKDIIISGGENISSLEIE 454
Cdd:PRK04813 338 KLPDGEQ--GEIVISGPSVSKGYLNNPEKTAEAFftFDGQpaYHTGDAGYL-EDGLLFYQGRIDFQIKLNGYRIELEEIE 414
|
490
....*....|....*
gi 489250734 455 EVLYQHPEVVEAAVV 469
Cdd:PRK04813 415 QNLRQSSYVESAVVV 429
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
3-529 |
6.12e-14 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 75.08 E-value: 6.12e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 3 VPTL---EHEL--APNEANH-VPLSPLS-FLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAI 75
Cdd:PRK10252 434 VDILlpgEYAQlaQVNATAVeIPETTLSaLVAQQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSVAV 513
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 76 LAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRhcaarvlicdrefgavaqqalamlDAPPLLVGIDDDQAERA 155
Cdd:PRK10252 514 ALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLE------------------------DARPSLLITTADQLPRF 569
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 156 DLAHDLD---YEAFLAQGDpGRPLSAPQNEWQSIAInYTSGTTGDPKGVVLHHRgAYLN----------ACAGALIFQLG 222
Cdd:PRK10252 570 ADVPDLTslcYNAPLAPQG-AAPLQLSQPHHTAYII-FTSGSTGRPKGVMVGQT-AIVNrllwmqnhypLTADDVVLQKT 646
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 223 PRSiylwtlpmFHCNGWSHTWAVtLSGGthvCLRKVQPDAIHAAIAEHAVTH---LSAAPVVMSML------IHAEHASV 293
Cdd:PRK10252 647 PCS--------FDVSVWEFFWPF-IAGA---KLVMAEPEAHRDPLAMQQFFAeygVTTTHFVPSMLaafvasLTPEGARQ 714
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 294 PAVPVS-VITGGAAPPsaviAAMEARGFSITHA-----YGMTE-----SYGPStlclwqpGVDELPLEARAQFMSRQGVA 362
Cdd:PRK10252 715 SCASLRqVFCSGEALP----ADLCREWQQLTGAplhnlYGPTEaavdvSWYPA-------FGEELAAVRGSSVPIGYPVW 783
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 363 HPMLEeatVLDtDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATRAA-LADGWL------HTGDLAVLHPDGYVEIKD 435
Cdd:PRK10252 784 NTGLR---ILD-ARMRPVPPG--VAGDLYLTGIQLAQGYLGRPDLTASRfIADPFApgermyRTGDVARWLDDGAVEYLG 857
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 436 RAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGET------------PHAFVALRADALprttgddlvrwc 503
Cdd:PRK10252 858 RSDDQLKIRGQRIELGEIDRAMQALPDVEQAVTHACVINQAAATggdarqlvgylvSQSGLPLDTSAL------------ 925
|
570 580 590
....*....|....*....|....*....|..
gi 489250734 504 RERLAHfKAPRH------VTLTELPKTATGKI 529
Cdd:PRK10252 926 QAQLRE-RLPPHmvpvvlLQLDQLPLSANGKL 956
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
11-541 |
2.62e-13 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 73.28 E-value: 2.62e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 11 APNEANHVPLSPLsfLKRAAQVYPQRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGV 90
Cdd:PRK05691 1124 APCAPAQAWLPEL--LNEQARQTPERIALVWDGGSLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAI 1201
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 91 PGAGAVLVCINIRLEARSIAFILRHCAARVLICDRefgavaqQALAMLDAPPLLVGIDDDQaeradlahdLDYEAFLAQG 170
Cdd:PRK05691 1202 LKAGGAYVPLDPDYPAERLAYMLADSGVELLLTQS-------HLLERLPQAEGVSAIALDS---------LHLDSWPSQA 1265
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 171 dPGRPLSAPQNEWqsiaINYTSGTTGDPKGV-----VLHHRGAYLNAcagalIFQLGPRSIYLWTLPM-FHCNGWSHTWA 244
Cdd:PRK05691 1266 -PGLHLHGDNLAY----VIYTSGSTGQPKGVgnthaALAERLQWMQA-----TYALDDSDVLMQKAPIsFDVSVWECFWP 1335
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 245 vtLSGGTHVCLRKV----QPDAIHAAIAEHAVTHLSAAPVVMSMLIHAEHASVPAVPVSVITGGAAPPsaviAAMEARGF 320
Cdd:PRK05691 1336 --LITGCRLVLAGPgehrDPQRIAELVQQYGVTTLHFVPPLLQLFIDEPLAAACTSLRRLFSGGEALP----AELRNRVL 1409
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 321 SITHAYGMTESYGPSTLCL----WQPGVDElplearaqfMSRQGVAHPMLEEAT-VLDTDTgRPVPADgrTLGELVVRGN 395
Cdd:PRK05691 1410 QRLPQVQLHNRYGPTETAInvthWQCQAED---------GERSPIGRPLGNVLCrVLDAEL-NLLPPG--VAGELCIGGA 1477
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 396 TVMKGYLHNPEATR---AALADG-----WLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAA 467
Cdd:PRK05691 1478 GLARGYLGRPALTAerfVPDPLGedgarLYRTGDRARWNADGALEYLGRLDQQVKLRGFRVEPEEIQARLLAQPGVAQAA 1557
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 489250734 468 VVARPDSrwgETPHAFVALRADALPRTTGDDLVRWCRERLAHFKAPRH-VTLTELPKTATGKIQKFVLRE--WARQE 541
Cdd:PRK05691 1558 VLVREGA---AGAQLVGYYTGEAGQEAEAERLKAALAAELPEYMVPAQlIRLDQMPLGPSGKLDRRALPEpvWQQRE 1631
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
47-544 |
1.30e-12 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 70.51 E-value: 1.30e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 47 SYRQLHERSCALASALERVgVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLICDRE 126
Cdd:PRK08043 233 SYRKLLKKTLFVGRILEKY-SVEGERIGLMLPNATISAAVIFGASLRRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQ 311
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 127 FGAVAQqalaMLDAPPLL-----VGIDD--DQAERAD----LAHDLDyeaflaqgdPGRPLSAPQNEWQSIaINYTSGTT 195
Cdd:PRK08043 312 FLDKGK----LWHLPEQLtqvrwVYLEDlkDDVTTADklwiFAHLLM---------PRLAQVKQQPEDAAL-ILFTSGSE 377
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 196 GDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLR------KVQPDaihaaiae 269
Cdd:PRK08043 378 GHPKGVVHSHKSLLANVEQIKTIADFTPNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYpsplhyRIVPE-------- 449
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 270 haVTHLSAAPVVMSMLIHAEHASVPAVP-----VSVITGGAAP--PSAVIAAMEARGFSITHAYGMTEsygpstlClwQP 342
Cdd:PRK08043 450 --LVYDRNCTVLFGTSTFLGNYARFANPydfarLRYVVAGAEKlqESTKQLWQDKFGLRILEGYGVTE-------C--AP 518
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 343 GVD-ELPLEARAQFMSRqgVAHPMleEATVLdtdtgrPVP--ADGrtlGELVVRGNTVMKGYL--HNPEATRAALAD--- 414
Cdd:PRK08043 519 VVSiNVPMAAKPGTVGR--ILPGM--DARLL------SVPgiEQG---GRLQLKGPNIMNGYLrvEKPGVLEVPTAEnar 585
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 415 -----GWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENIsSLEIEEVLYQ--HPEVVEAAvVARPDSRWGEtphAFVALR 487
Cdd:PRK08043 586 gemerGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV-SLEMVEQLALgvSPDKQHAT-AIKSDASKGE---ALVLFT 660
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 489250734 488 ADAlpRTTGDDLVRWCRER-LAHFKAPRHVT-LTELPKTATGKIQKFVLREWARQEEEA 544
Cdd:PRK08043 661 TDS--ELTREKLQQYAREHgVPELAVPRDIRyLKQLPLLGSGKPDFVTLKSMVDEPEQH 717
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
34-229 |
3.63e-12 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 69.05 E-value: 3.63e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 34 PQRDAVVY-----GARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARS 108
Cdd:PRK03584 98 DDRPAIIFrgedgPRRELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLATASLGAIWSSCSPDFGVQG 177
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 109 IAFILRHCAARVLI-CD------REF--GAVAQQALAMLDAPPLLVGID--DDQAERADLAHDLDYEAFLAQGDPGrpls 177
Cdd:PRK03584 178 VLDRFGQIEPKVLIaVDgyryggKAFdrRAKVAELRAALPSLEHVVVVPylGPAAAAAALPGALLWEDFLAPAEAA---- 253
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....*..
gi 489250734 178 APQNEW----QSIAINYTSGTTGDPKGVVLHHRGAYLNAC-AGALIFQLGPRSIYLW 229
Cdd:PRK03584 254 ELEFEPvpfdHPLWILYSSGTTGLPKCIVHGHGGILLEHLkELGLHCDLGPGDRFFW 310
|
|
| PRK12582 |
PRK12582 |
acyl-CoA synthetase; Provisional |
19-444 |
3.75e-12 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237144 [Multi-domain] Cd Length: 624 Bit Score: 68.92 E-value: 3.75e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 19 PLSPLSFLKRAAQVYP------QRDAVVYGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPE----MLEA-H 87
Cdd:PRK12582 48 PRSIPHLLAKWAAEAPdrpwlaQREPGHGQWRKVTYGEAKRAVDALAQALLDLGLDPGRPVMILSGNSIEhalmTLAAmQ 127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 88 YGVPGAGavlVCINIRLEARSIAfILRHCAARV---LICDREfGAVAQQALAMLDA--PPLLVGIDDDQAER----ADLA 158
Cdd:PRK12582 128 AGVPAAP---VSPAYSLMSHDHA-KLKHLFDLVkprVVFAQS-GAPFARALAALDLldVTVVHVTGPGEGIAsiafADLA 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 159 H---DLDYEAFLAQGDPGrplsapqnewqSIA-INYTSGTTGDPKGVVLHHRGAYLNACAGALIF---QLGPRSIYLWTL 231
Cdd:PRK12582 203 AtppTAAVAAAIAAITPD-----------TVAkYLFTSGSTGMPKAVINTQRMMCANIAMQEQLRprePDPPPPVSLDWM 271
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 232 PMFHCNGWSHTW-AVTLSGGTHVC----------------LRKVQPdaihaaiaehavTHLSAAPVVMSMLIHAEHASvP 294
Cdd:PRK12582 272 PWNHTMGGNANFnGLLWGGGTLYIddgkplpgmfeetirnLREISP------------TVYGNVPAGYAMLAEAMEKD-D 338
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 295 AVPVSVIT-------GGAAPPSAVIAAMEA-------RGFSITHAYGMTESYGPSTLCLW---QPGVDELPLEaraqfms 357
Cdd:PRK12582 339 ALRRSFFKnlrlmayGGATLSDDLYERMQAlavrttgHRIPFYTGYGATETAPTTTGTHWdteRVGLIGLPLP------- 411
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 358 rqGVAHPMLeeatvldtdtgrPVpadGRTLgELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGDLAVlhpdgYVEIKDR 436
Cdd:PRK12582 412 --GVELKLA------------PV---GDKY-EVRVKGPNVTPGYHKDPELTAAAFdEEGFYRLGDAAR-----FVDPDDP 468
|
....*...
gi 489250734 437 AKDIIISG 444
Cdd:PRK12582 469 EKGLIFDG 476
|
|
| FCS |
cd05921 |
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ... |
44-529 |
5.69e-12 |
|
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.
Pssm-ID: 341245 [Multi-domain] Cd Length: 561 Bit Score: 68.23 E-value: 5.69e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 44 RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFI-LRHCAAR--- 119
Cdd:cd05921 24 RRVTYAEALRQVRAIAQGLLDLGLSAERPLLILSGNSIEHALMALAAMYAGVPAAPVSPAYSLMSQDLAkLKHLFELlkp 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 120 --VLICDrefGAVAQQALA--MLDAPPLLV----GIDDDQAERADLAHdldyEAFLAQGDPGRPLSAPQnewqSIA-INY 190
Cdd:cd05921 104 glVFAQD---AAPFARALAaiFPLGTPLVVsrnaVAGRGAISFAELAA----TPPTAAVDAAFAAVGPD----TVAkFLF 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 191 TSGTTGDPKGVVLHHRGAYLNAC--AGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRKVQPDAIHAAIA 268
Cdd:cd05921 173 TSGSTGLPKAVINTQRMLCANQAmlEQTYPFFGEEPPVLVDWLPWNHTFGGNHNFNLVLYNGGTLYIDDGKPMPGGFEET 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 269 EHAVTHLS-----AAPVVMSMLIHAEHASvPAVPVSVIT-------GGAAPPSAVIAAMEA-------RGFSITHAYGMT 329
Cdd:cd05921 253 LRNLREISptvyfNVPAGWEMLVAALEKD-EALRRRFFKrlklmfyAGAGLSQDVWDRLQAlavatvgERIPMMAGLGAT 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 330 ESYGPSTLCLW---QPGVDELPLearaqfmsrQGVAHPMleeatvldtdtgrpVPADGRTlgELVVRGNTVMKGYLHNPE 406
Cdd:cd05921 332 ETAPTATFTHWpteRSGLIGLPA---------PGTELKL--------------VPSGGKY--EVRVKGPNVTPGYWRQPE 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 407 ATRAAL-ADGWLHTGDLAVL-HPD----GYVEIKDRAKDIIISGGENIS--SLEIEEVLYQHPeVVEAAVVARPDsrwGE 478
Cdd:cd05921 387 LTAQAFdEEGFYCLGDAAKLaDPDdpakGLVFDGRVAEDFKLASGTWVSvgPLRARAVAACAP-LVHDAVVAGED---RA 462
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 489250734 479 TPHAFVALRADALPRTTG------------DDLVRWCRERLAHFK-----APRHVT----LTELPKTATGKI 529
Cdd:cd05921 463 EVGALVFPDLLACRRLVGlqeasdaevlrhAKVRAAFRDRLAALNgeatgSSSRIArallLDEPPSIDKGEI 534
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
404-547 |
6.44e-12 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 67.37 E-value: 6.44e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 404 NPEATRAALADGWLHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETphaf 483
Cdd:PRK08308 280 APEEIVVKMGDKEIFTKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGER---- 355
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489250734 484 VALRADALPRTTGDDLVRWCRERLAHFKAPRHVT-LTELPKTATGKIqkfvlrewARQEEEAGDA 547
Cdd:PRK08308 356 VKAKVISHEEIDPVQLREWCIQHLAPYQVPHEIEsVTEIPKNANGKV--------SRKLLELGEV 412
|
|
| PRK06334 |
PRK06334 |
long chain fatty acid--[acyl-carrier-protein] ligase; Validated |
47-460 |
8.56e-12 |
|
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
Pssm-ID: 180533 [Multi-domain] Cd Length: 539 Bit Score: 67.53 E-value: 8.56e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 47 SYRQLHERSCALASaleRVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSiafiLRHCAA-----RVL 121
Cdd:PRK06334 47 SYNQVRKAVIALAT---KVSKYPDQHIGIMMPASAGAYIAYFATLLSGKIPVMINWSQGLRE----VTACANlvgvtHVL 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 122 ICDREFGAVAQQALAMLDAPPLLVGIDDDQAE-------RADLAHDLDYEAFLAQGDpgrplSAPQNEWQSIAINYTSGT 194
Cdd:PRK06334 120 TSKQLMQHLAQTHGEDAEYPFSLIYMEEVRKElsfwekcRIGIYMSIPFEWLMRWFG-----VSDKDPEDVAVILFTSGT 194
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 195 TGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCL--RKVQPDAIHAAIAEHAV 272
Cdd:PRK06334 195 EKLPKGVPLTHANLLANQRACLKFFSPKEDDVMMSFLPPFHAYGFNSCTLFPLLSGVPVVFayNPLYPKKIVEMIDEAKV 274
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 273 THLSAAPVVMSMLIHA---EHASVPAVPVSVITGGAAPPSavIAAMEARGF---SITHAYGMTESYGPSTLclwqpGVDE 346
Cdd:PRK06334 275 TFLGSTPVFFDYILKTakkQESCLPSLRFVVIGGDAFKDS--LYQEALKTFphiQLRQGYGTTECSPVITI-----NTVN 347
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 347 LPLEARAQFMSRQGVahpmleEATVLDTDTGRPVPAdGRTlGELVVRGNTVMKGYLHN-PEATRAALA-DGWLHTGDLAV 424
Cdd:PRK06334 348 SPKHESCVGMPIRGM------DVLIVSEETKVPVSS-GET-GLVLTRGTSLFSGYLGEdFGQGFVELGgETWYVTGDLGY 419
|
410 420 430
....*....|....*....|....*....|....*.
gi 489250734 425 LHPDGYVEIKDRAKDIIISGGENISSLEIEEVLYQH 460
Cdd:PRK06334 420 VDRHGELFLKGRLSRFVKIGAEMVSLEALESILMEG 455
|
|
| PRK07769 |
PRK07769 |
long-chain-fatty-acid--CoA ligase; Validated |
35-447 |
2.09e-11 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 181109 [Multi-domain] Cd Length: 631 Bit Score: 66.68 E-value: 2.09e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 35 QRDAVvygARRYSYRQLHERSCALASALERVGvQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCI--------NIRLEA 106
Cdd:PRK07769 48 ERDGV---ARDLTWSQFGARNRAVGARLQQVT-KPGDRVAILAPQNLDYLIAFFGALYAGRIAVPLfdpaepghVGRLHA 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 107 rsiafILRHCAARVLICDREFGAVAQQALAMLDAP--PLLVGID---DDQAER-ADLAHDLDYEAFLaqgdpgrplsapq 180
Cdd:PRK07769 124 -----VLDDCTPSAILTTTDSAEGVRKFFRARPAKerPRVIAVDavpDEVGATwVPPEANEDTIAYL------------- 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 181 newqsiaiNYTSGTTGDPKGVVLHHRGAYLNACA--GALIFQLGPRSIYlWtLPMFHCNGWSHTWAVTLsGGTHVCLrkV 258
Cdd:PRK07769 186 --------QYTSGSTRIPAGVQITHLNLPTNVLQviDALEGQEGDRGVS-W-LPFFHDMGLITVLLPAL-LGHYITF--M 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 259 QPdAIHAAIAEHAVTHLSAAPVVMSMLIHA------EHASVPAVP-----------VSVITGGAAPPSavIAAM----EA 317
Cdd:PRK07769 253 SP-AAFVRRPGRWIRELARKPGGTGGTFSAapnfafEHAAARGLPkdgeppldlsnVKGLLNGSEPVS--PASMrkfnEA 329
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 318 ---RGF---SITHAYGMTESygpsTLCLWQPGVDElplEARAQFMSRQ----------------GVAH------PMLEEA 369
Cdd:PRK07769 330 fapYGLpptAIKPSYGMAEA----TLFVSTTPMDE---EPTVIYVDRDelnagrfvevpadapnAVAQvsagkvGVSEWA 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 370 TVLDTDTGRPVPaDGRtLGELVVRGNTVMKGYLHNPEATRAAL------------ADG------WLHTGDLAVLHpDGYV 431
Cdd:PRK07769 403 VIVDPETASELP-DGQ-IGEIWLHGNNIGTGYWGKPEETAATFqnilksrlseshAEGapddalWVRTGDYGVYF-DGEL 479
|
490
....*....|....*.
gi 489250734 432 EIKDRAKDIIISGGEN 447
Cdd:PRK07769 480 YITGRVKDLVIIDGRN 495
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
14-535 |
2.25e-11 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 67.12 E-value: 2.25e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 14 EANHVPLSPLSFLKRAAQVYPQRDAVVY------GARRYSYRQLHERSCALASALERVGvQAGERVAILAPNIPEMLEAH 87
Cdd:PRK05691 3 DAFELPLTLVQALQRRAAQTPDRLALRFladdpgEGVVLSYRDLDLRARTIAAALQARA-SFGDRAVLLFPSGPDYVAAF 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 88 YGVPGAGAVLVCI-----NIRLEARSIAFILRHCAARVLICDREFGAVAQQ--ALAMLDAPPLLV--GIDDDQAERADla 158
Cdd:PRK05691 82 FGCLYAGVIAVPAyppesARRHHQERLLSIIADAEPRLLLTVADLRDSLLQmeELAAANAPELLCvdTLDPALAEAWQ-- 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 159 hdldyeaflaqgdpgrplsAPQNEWQSIA-INYTSGTTGDPKGVVLHHRGAYLNAcagALIFQ-----LGPRSIYLWTLP 232
Cdd:PRK05691 160 -------------------EPALQPDDIAfLQYTSGSTALPKGVQVSHGNLVANE---QLIRHgfgidLNPDDVIVSWLP 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 233 MFHCNGWSHTWAVTLSGGTHVCLRKVQPDAIHAAIAEHAVTH----LSAAPVVMSMLIHAE--HASVPAVPVS---VITG 303
Cdd:PRK05691 218 LYHDMGLIGGLLQPIFSGVPCVLMSPAYFLERPLRWLEAISEyggtISGGPDFAYRLCSERvsESALERLDLSrwrVAYS 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 304 GAAPPSAVIAAMEARGFSithAYGMTE-----SYGPSTLCLW----QPGVDELPLEARAQFMSRQgVAHPMlEEATVLDT 374
Cdd:PRK05691 298 GSEPIRQDSLERFAEKFA---ACGFDPdsffaSYGLAEATLFvsggRRGQGIPALELDAEALARN-RAEPG-TGSVLMSC 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 375 DTGRP-------------VPADGRtLGELVVRGNTVMKGYLHNPEATRAALA--DG--WLHTGDLAVLHpDGYVEIKDRA 437
Cdd:PRK05691 373 GRSQPghavlivdpqsleVLGDNR-VGEIWASGPSIAHGYWRNPEASAKTFVehDGrtWLRTGDLGFLR-DGELFVTGRL 450
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 438 KDIIISGGENISSLEIEEVLYQHPEVVE----AAVVARPDSRWGETPHAFVALRADALprTTGDDLVRWCRERLA--HFK 511
Cdd:PRK05691 451 KDMLIVRGHNLYPQDIEKTVEREVEVVRkgrvAAFAVNHQGEEGIGIAAEISRSVQKI--LPPQALIKSIRQAVAeaCQE 528
|
570 580
....*....|....*....|....*..
gi 489250734 512 APRHVTLTE---LPKTATGKIQKFVLR 535
Cdd:PRK05691 529 APSVVLLLNpgaLPKTSSGKLQRSACR 555
|
|
| PRK05851 |
PRK05851 |
long-chain-fatty acid--ACP ligase MbtM; |
313-501 |
2.32e-11 |
|
long-chain-fatty acid--ACP ligase MbtM;
Pssm-ID: 180289 [Multi-domain] Cd Length: 525 Bit Score: 66.33 E-value: 2.32e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 313 AAMEARGF---SITHAYGMTESYGPSTLClwQPG----VDEL--PLEARAQFMSRQGVAHPMLEeatVLDTDTGRPVPAD 383
Cdd:PRK05851 294 TAMAPFGFdagAAAPSYGLAESTCAVTVP--VPGiglrVDEVttDDGSGARRHAVLGNPIPGME---VRISPGDGAAGVA 368
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 384 GRTLGELVVRGNTVMKGYLHNPEATRaalaDGWLHTGDLAVLHPDGYVeIKDRAKDIIISGGENISSLEIEEVLYQHPEV 463
Cdd:PRK05851 369 GREIGEIEIRGASMMSGYLGQAPIDP----DDWFPTGDLGYLVDGGLV-VCGRAKELITVAGRNIFPTEIERVAAQVRGV 443
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 464 VEAAVVA--------------------------------RPDSRWGETPHAFVALRADALPRTTGDDLVR 501
Cdd:PRK05851 444 REGAVVAvgtgegsarpglviaaefrgpdeagarsevvqRVASECGVVPSDVVFVAPGSLPRTSSGKLRR 513
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
44-535 |
3.03e-11 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 65.52 E-value: 3.03e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 44 RRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARVLIc 123
Cdd:cd05939 2 RHWTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALI- 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 124 dreFGAVAQQALAMLDAPPLLVGIDddqaeradlAHD-LDYEaflaqgdpgrplsapqnewqsiainYTSGTTGDPKGVV 202
Cdd:cd05939 81 ---FNLLDPLLTQSSTEPPSQDDVN---------FRDkLFYI-------------------------YTSGTTGLPKAAV 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 203 LHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHTWAVTLSGGTHVCLRK-----------VQPDAIHAAIAEHA 271
Cdd:cd05939 124 IVHSRYYRIAAGAYYAFGMRPEDVVYDCLPLYHSAGGIMGVGQALLHGSTVVIRKkfsasnfwddcVKYNCTIVQYIGEI 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 272 VTHLSAAPVVMSMLIHAehasvpavpVSVITGGAAPPSavIAAMEARGFSITHA---YGMTEsyGPSTLCLWQPGVdelp 348
Cdd:cd05939 204 CRYLLAQPPSEEEQKHN---------VRLAVGNGLRPQ--IWEQFVRRFGIPQIgefYGATE--GNSSLVNIDNHV---- 266
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 349 leARAQFMSRQGVA-HPMleEATVLDTDTGRP--------VPADGRTLGELV---VRGNTVMK--GYLhNPEATRAALAD 414
Cdd:cd05939 267 --GACGFNSRILPSvYPI--RLIKVDEDTGELirdsdglcIPCQPGEPGLLVgkiIQNDPLRRfdGYV-NEGATNKKIAR 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 415 GWLHTGDLA-----VLHPD--GYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVvarpdsrWG-ETPH----A 482
Cdd:cd05939 342 DVFKKGDSAflsgdVLVMDelGYLYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLEDVVV-------YGvEVPGvegrA 414
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 489250734 483 FVALRADALPRTTGDDLVRWCRERLAHFKAPRHVTL-TELPKTATGKIQKFVLR 535
Cdd:cd05939 415 GMAAIVDPERKVDLDRFSAVLAKSLPPYARPQFIRLlPEVDKTGTFKLQKTDLQ 468
|
|
| PRK08180 |
PRK08180 |
feruloyl-CoA synthase; Reviewed |
19-421 |
9.81e-11 |
|
feruloyl-CoA synthase; Reviewed
Pssm-ID: 236175 [Multi-domain] Cd Length: 614 Bit Score: 64.51 E-value: 9.81e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 19 PLSPLS-----FLKRAAQVYPQRDAVVY-----GARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPE----ML 84
Cdd:PRK08180 33 PLGDYPrrltdRLVHWAQEAPDRVFLAErgadgGWRRLTYAEALERVRAIAQALLDRGLSAERPLMILSGNSIEhallAL 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 85 EAHY-GVPGAgAV-----LVcinirlearSIAFI-LRHCAAR-----VLICDREFGAVAQQALAMLDAPplLVGIDDDQA 152
Cdd:PRK08180 113 AAMYaGVPYA-PVspaysLV---------SQDFGkLRHVLELltpglVFADDGAAFARALAAVVPADVE--VVAVRGAVP 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 153 ERADLAhdldYEAFLAQGDPGRPLSA-----PQnewqSIA-INYTSGTTGDPKGVVLHHRgaylNACAGALI-------F 219
Cdd:PRK08180 181 GRAATP----FAALLATPPTAAVDAAhaavgPD----TIAkFLFTSGSTGLPKAVINTHR----MLCANQQMlaqtfpfL 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 220 QLGPRSIYLWtLPMFHCNGWSHTWAVTL-SGGTHVC----------------LRKVQPdaihaaiaehavTHLSAAPVVM 282
Cdd:PRK08180 249 AEEPPVLVDW-LPWNHTFGGNHNLGIVLyNGGTLYIddgkptpggfdetlrnLREISP------------TVYFNVPKGW 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 283 SMLIHA--EHASVPAVPVSVIT----GGAAPPSAVIA-----AMEARG--FSITHAYGMTESYGPSTLCLWQ---PGVDE 346
Cdd:PRK08180 316 EMLVPAleRDAALRRRFFSRLKllfyAGAALSQDVWDrldrvAEATCGerIRMMTGLGMTETAPSATFTTGPlsrAGNIG 395
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 489250734 347 LPLearaqfmsrQGVAHPMleeatvldtdtgrpVPADGRTlgELVVRGNTVMKGYLHNPEATRAAL-ADGWLHTGD 421
Cdd:PRK08180 396 LPA---------PGCEVKL--------------VPVGGKL--EVRVKGPNVTPGYWRAPELTAEAFdEEGYYRSGD 446
|
|
| PLN03051 |
PLN03051 |
acyl-activating enzyme; Provisional |
163-541 |
2.88e-09 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215552 [Multi-domain] Cd Length: 499 Bit Score: 59.44 E-value: 2.88e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 163 YEAFLAQGDPGRPLSAPQNewQSIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSIYLWTLPMFHCNGWSHT 242
Cdd:PLN03051 101 AAQGSVGGNEYSPVYAPVE--SVTNILFSSGTTGEPKAIPWTHLSPLRCASDGWAHMDIQPGDVVCWPTNLGWMMGPWLL 178
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 243 WAVTLSGGThVCLRKVQPDAI--HAAIAEHAVTHLSAAPVVMSMLIH-----AEHASVPAVPVSVITGGAAPPSAVIAAM 315
Cdd:PLN03051 179 YSAFLNGAT-LALYGGAPLGRgfGKFVQDAGVTVLGLVPSIVKAWRHtgafaMEGLDWSKLRVFASTGEASAVDDVLWLS 257
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 316 EARGFS--ITHAYGMTE---SYGPSTLClwQPgvdelplEARAQFMSrqgvahPMLEEATVLDTDTGRPVPADGRTLGEL 390
Cdd:PLN03051 258 SVRGYYkpVIEYCGGTElasGYISSTLL--QP-------QAPGAFST------ASLGTRFVLLNDNGVPYPDDQPCVGEV 322
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 391 VVRgnTVMKGY---LHNPEaTRAALADGW----------LHTGDLAVLHPDGYVEIKDRAKDIIISGGENISSLEIEEVL 457
Cdd:PLN03051 323 ALA--PPMLGAsdrLLNAD-HDKVYYKGMpmygskgmplRRHGDIMKRTPGGYFCVQGRADDTMNLGGIKTSSVEIERAC 399
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 458 YQHPE-VVEAAVVARPDSRWGETPHAFVALRADA---LPRTTGDDLVRWCRERLAH-----FKAPRHVTLTELPKTATGK 528
Cdd:PLN03051 400 DRAVAgIAETAAVGVAPPDGGPELLVIFLVLGEEkkgFDQARPEALQKKFQEAIQTnlnplFKVSRVKIVPELPRNASNK 479
|
410
....*....|...
gi 489250734 529 IQKFVLREWARQE 541
Cdd:PLN03051 480 LLRRVLRDQLKKE 492
|
|
| PRK05850 |
PRK05850 |
acyl-CoA synthetase; Validated |
21-454 |
3.32e-09 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235624 [Multi-domain] Cd Length: 578 Bit Score: 59.57 E-value: 3.32e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 21 SPLSFLKRAAQVYPQRDAVVY--------G-ARRYSYRQLHERSCALASALERVGVqAGERVAILAPNIPEMLEAHYGVP 91
Cdd:PRK05850 2 SVPSLLRERASLQPDDAAFTFidyeqdpaGvAETLTWSQLYRRTLNVAEELRRHGS-TGDRAVILAPQGLEYIVAFLGAL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 92 GAGAVLVCINIRL----EARSIAfILRHCAARVLICD-------REFGAVAQqalamLDAPPLLVGIDddqaeradlAHD 160
Cdd:PRK05850 81 QAGLIAVPLSVPQggahDERVSA-VLRDTSPSVVLTTsavvddvTEYVAPQP-----GQSAPPVIEVD---------LLD 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 161 LDyeaflaqgdPGRPLSAPQNEWQSIA-INYTSGTTGDPKGVVLHHRG----------AYLNACAGALIFqlgPRSIYLW 229
Cdd:PRK05850 146 LD---------SPRGSDARPRDLPSTAyLQYTSGSTRTPAGVMVSHRNvianfeqlmsDYFGDTGGVPPP---DTTVVSW 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 230 tLPMFHCNGWSHTWAVTLSGGTHVCLrkvqpdaihaaiaehavthlsAAPVV--------MSMLihAEH-ASVPAVP--- 297
Cdd:PRK05850 214 -LPFYHDMGLVLGVCAPILGGCPAVL---------------------TSPVAflqrparwMQLL--ASNpHAFSAAPnfa 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 298 --------------------VSVITGGA--APPSAV------IAAMEARGFSITHAYGMTESygpsTLCLWQPGVDELPL 349
Cdd:PRK05850 270 felavrktsdddmagldlggVLGIISGSerVHPATLkrfadrFAPFNLRETAIRPSYGLAEA----TVYVATREPGQPPE 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 350 EAR--AQFMSR------------QGVAHPMLEEATV--LDTDTGRPVPADgrTLGELVVRGNTVMKGYLHNPEATR---- 409
Cdd:PRK05850 346 SVRfdYEKLSAghakrcetgggtPLVSYGSPRSPTVriVDPDTCIECPAG--TVGEIWVHGDNVAAGYWQKPEETErtfg 423
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 489250734 410 AALADG--------WLHTGDLAVLHpDGYVEIKDRAKDIIISGGENISSLEIE 454
Cdd:PRK05850 424 ATLVDPspgtpegpWLRTGDLGFIS-EGELFIVGRIKDLLIVDGRNHYPDDIE 475
|
|
| PRK12476 |
PRK12476 |
putative fatty-acid--CoA ligase; Provisional |
47-500 |
6.46e-07 |
|
putative fatty-acid--CoA ligase; Provisional
Pssm-ID: 171527 [Multi-domain] Cd Length: 612 Bit Score: 52.05 E-value: 6.46e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 47 SYRQLHERSCALASALERVgVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCI---NIRLEARSIAFILRHCAARVLIC 123
Cdd:PRK12476 70 TWTQLGVRLRAVGARLQQV-AGPGDRVAILAPQGIDYVAGFFAAIKAGTIAVPLfapELPGHAERLDTALRDAEPTVVLT 148
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 124 DrefGAVAQQALAMLDA-----PPLLVGID---DDQAERADLAH-DLDYEAFLaqgdpgrplsapqnewqsiaiNYTSGT 194
Cdd:PRK12476 149 T---TAAAEAVEGFLRNlprlrRPRVIAIDaipDSAGESFVPVElDTDDVSHL---------------------QYTSGS 204
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 195 TGDPKGVVLHHRGAYLNACAGALIFQLGPRSIY--LWtLPMFHCNGWSHTWAVTLSGGtHVCL-------RKVQPDAIHA 265
Cdd:PRK12476 205 TRPPVGVEITHRAVGTNLVQMILSIDLLDRNTHgvSW-LPLYHDMGLSMIGFPAVYGG-HSTLmsptafvRRPQRWIKAL 282
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 266 AIAEHAVTHLSAAPVVMSMLihAEHASVPA----VPVS--VITGGAAPPSavIAAMEArgFSITHA-YGMTE-----SYG 333
Cdd:PRK12476 283 SEGSRTGRVVTAAPNFAYEW--AAQRGLPAegddIDLSnvVLIIGSEPVS--IDAVTT--FNKAFApYGLPRtafkpSYG 356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 334 PSTLCLWQPGVDElPLEARAQFMSRQGVA----------HPML------------EEATVLDTDTGRPVPaDGRtLGELV 391
Cdd:PRK12476 357 IAEATLFVATIAP-DAEPSVVYLDREQLGagravrvaadAPNAvahvscgqvarsQWAVIVDPDTGAELP-DGE-VGEIW 433
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 392 VRGNTVMKGYLHNPEATR--------AALADG-----------WLHTGDLAVlHPDGYVEIKDRAKDIIISGGENissle 452
Cdd:PRK12476 434 LHGDNIGRGYWGRPEETErtfgaklqSRLAEGshadgaaddgtWLRTGDLGV-YLDGELYITGRIADLIVIDGRN----- 507
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 489250734 453 ieevlyQHPEVVEAAVV-ARPDSRWGetphaFVAlrADALPRTTGDDLV 500
Cdd:PRK12476 508 ------HYPQDIEATVAeASPMVRRG-----YVT--AFTVPAEDNERLV 543
|
|
| Dip2 |
cd05905 |
Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of ... |
46-213 |
8.96e-07 |
|
Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of the adenylate forming enzyme family, including insect luciferase, acetyl CoA ligases and the adenylation domain of nonribosomal peptide synthetases (NRPS). However, its function may have diverged from other members of the superfamily. In mouse embryo, Dip2 homolog A plays an important role in the development of both vertebrate and invertebrate nervous systems. Dip2A appears to regulate cell growth and the arrangement of cells in organs. Biochemically, Dip2A functions as a receptor of FSTL1, an extracellular glycoprotein, and may play a role as a cardiovascular protective agent.
Pssm-ID: 341231 [Multi-domain] Cd Length: 571 Bit Score: 51.58 E-value: 8.96e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 46 YSYRQLHERSCALASAL-ERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEARSIAFILRHCAARvlicd 124
Cdd:cd05905 15 LTWGKLLSRAEKIAAVLqKKVGLKPGDRVALMYPDPLDFVAAFYGCLYAGVVPIPIEPPDISQQLGFLLGTCKVR----- 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 125 refgaVAQQALAMLDAPPLLVGIDDDQAERADLAHdldYEAFLAQGDPGRPLSAPQNEWQSI---------AINYTSGTT 195
Cdd:cd05905 90 -----VALTVEACLKGLPKKLLKSKTAAEIAKKKG---WPKILDFVKIPKSKRSKLKKWGPHpptrdgdtaYIEYSFSSD 161
|
170
....*....|....*...
gi 489250734 196 GDPKGVVLHHRgAYLNAC 213
Cdd:cd05905 162 GSLSGVAVSHS-SLLAHC 178
|
|
| alpha_am_amid |
TIGR03443 |
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are ... |
30-205 |
3.98e-05 |
|
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), product of the LYS2 gene. It is also called alpha-aminoadipate reductase. In fungi, lysine is synthesized via aminoadipate. Currently, all members of this family are fungal.
Pssm-ID: 274582 [Multi-domain] Cd Length: 1389 Bit Score: 46.60 E-value: 3.98e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 30 AQVYPQRDAVV---------YGARRYSYRQLHERSCALASALERVGVQAGERVAILAPNIPEMLEAHYGVPGAGAVLVCI 100
Cdd:TIGR03443 246 AEKHPDRTCVVetpsfldpsSKTRSFTYKQINEASNILAHYLLKTGIKRGDVVMIYAYRGVDLVVAVMGVLKAGATFSVI 325
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489250734 101 ---------NIRLE-ARSIAFI-LRhcAARVL------ICDREFGAVAQ-QALAMLDAPPlLVGIDDDQAERADLAHdld 162
Cdd:TIGR03443 326 dpaypparqTIYLSvAKPRALIvIE--KAGTLdqlvrdYIDKELELRTEiPALALQDDGS-LVGGSLEGGETDVLAP--- 399
|
170 180 190 200
....*....|....*....|....*....|....*....|....*...
gi 489250734 163 YEAFlaQGDP-----GrPLSAPqnewqsiAINYTSGTTGDPKGVVLHH 205
Cdd:TIGR03443 400 YQAL--KDTPtgvvvG-PDSNP-------TLSFTSGSEGIPKGVLGRH 437
|
|
| PTZ00342 |
PTZ00342 |
acyl-CoA synthetase; Provisional |
388-448 |
1.76e-04 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240370 [Multi-domain] Cd Length: 746 Bit Score: 44.32 E-value: 1.76e-04
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 489250734 388 GELVVRGNTVMKGYLHNPEATRAALA-DGWLHTGDLAVLHPDGYVEIKDRAKDII-ISGGENI 448
Cdd:PTZ00342 542 GELLIKSDSIFSGYFLEKEQTKNAFTeDGYFKTGDIVQINKNGSLTFLDRSKGLVkLSQGEYI 604
|
|
| |
