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Conserved domains on  [gi|489193357|ref|WP_003102692|]
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MULTISPECIES: sterol desaturase family protein [Pseudomonas]

Protein Classification

sterol desaturase family protein( domain architecture ID 11459499)

sterol desaturase family protein is an oxidoreductase belonging to the fatty acid hydroxylase superfamily, similar to C-5 sterol desaturase, which catalyzes a dehydrogenation reaction to introduce a C5-6 double bond into substrates such as lathosterol and the B ring of ergosterol

EC:  1.-.-.-
Gene Ontology:  GO:0006629|GO:0016491|GO:0008610
SCOP:  3002435

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
 73-226 5.65e-38

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


:

Pssm-ID: 442238  Cd Length: 158  Bit Score: 130.44  E-value: 5.65e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489193357  73 AGFAAYFVNTFVTYWWHRARHANDTLWRlFHQLHHAPQRIEVFTSFYKHPTEMVFNSLLGsFVAYVVMGISIEAGAYYIM 152
Cdd:COG3000    1 QFLLAFLLLDFVYYWLHRLSHRVPLLWR-FHAVHHSSERMNLLTALRFHPLEILLSALLF-LLPLALLGFPPEAVLLYLA 78

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489193357 153 FAALGEMFYHSNLRTP--HVLGYLFQRPEMHRIHHQRDRHE--CNYS-DFPIWDMLFGTYENPRRIDEPQGFAGDKEQQ 226
Cdd:COG3000   79 LNLLYQFFNHSNIRLPldGPLEYVFVTPSHHRVHHSRNPEYldKNYGgILSIWDRLFGTYRPEEREPIRYGLTGPPRPR 157
 
Name Accession Description Interval E-value
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
 73-226 5.65e-38

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 130.44  E-value: 5.65e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489193357  73 AGFAAYFVNTFVTYWWHRARHANDTLWRlFHQLHHAPQRIEVFTSFYKHPTEMVFNSLLGsFVAYVVMGISIEAGAYYIM 152
Cdd:COG3000    1 QFLLAFLLLDFVYYWLHRLSHRVPLLWR-FHAVHHSSERMNLLTALRFHPLEILLSALLF-LLPLALLGFPPEAVLLYLA 78

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489193357 153 FAALGEMFYHSNLRTP--HVLGYLFQRPEMHRIHHQRDRHE--CNYS-DFPIWDMLFGTYENPRRIDEPQGFAGDKEQQ 226
Cdd:COG3000   79 LNLLYQFFNHSNIRLPldGPLEYVFVTPSHHRVHHSRNPEYldKNYGgILSIWDRLFGTYRPEEREPIRYGLTGPPRPR 157
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 75-206 3.81e-26

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 99.04  E-value: 3.81e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489193357   75 FAAYFVNTFVTYWWHRARHANDTLWRLFHQLHHAPQRIEVFTSFYKHPTEMVFNSLLGsFVAYVVMGISIEAGAYYIMFA 154
Cdd:pfam04116   2 LLGLLLFDFLFYWVHRLLHRLPWLWRRFHKVHHSSEAPNALTALRFHPLEALLFALLV-LLPLLLLGLPLLAFLLGLLLG 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 489193357  155 ALGEMFYHSN--LRTPHVLGYLFQRPEMHRIHHQRDrHECNYSD-FPIWDMLFGT 206
Cdd:pfam04116  81 TLWYLFIHSGllFPLPWLLKRLLGTPRFHRLHHSKN-EEYNFGVtFPLWDRLFGT 134
PLN02869 PLN02869
fatty aldehyde decarbonylase
 83-206 3.82e-05

fatty aldehyde decarbonylase


Pssm-ID: 166510 [Multi-domain]  Cd Length: 620  Bit Score: 44.45  E-value: 3.82e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489193357  83 FVTYWWHRARHaNDTLWRLFHQLHHAPQRIEVFTSFYkHPtemvFNSLLGSFVAY-------VVMGI-SIEAGAYYIMFA 154
Cdd:PLN02869 140 FLYYWLHRALH-HHYLYSRYHSHHHSSIVTEPITSVI-HP----FAEHIAYFLLFaipllttIFTGTaSIAAFFGYISYI 213

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 489193357 155 ALGEMFYHSNLR-TPHV-------LGYLFQRPEMHRIHHQRDRheCNYSDF-PIWDMLFGT 206
Cdd:PLN02869 214 DFMNNMGHCNFElIPKWlfsifppLKYLMYTPSYHSLHHTQFR--TNYSLFmPIYDYIYGT 272
SCM_chp_ScmC TIGR04249
SynChlorMet cassette protein ScmC; A biosynthesis cassette found in Syntrophobacter ...
 65-115 4.29e-03

SynChlorMet cassette protein ScmC; A biosynthesis cassette found in Syntrophobacter fumaroxidans MPOB, Chlorobium limicola DSM 245, Methanocella paludicola SANAE, and delta proteobacterium NaphS2 contains two PqqE-like radical SAM/SPASM domain proteins, a PqqD homolog, and a conserved hypothetical protein. These components suggest modification of a ribosomally produced peptide precursor, but the precursor has not been identified. Members of this family are designated ScmC.


Pssm-ID: 275080  Cd Length: 292  Bit Score: 37.58  E-value: 4.29e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 489193357   65 SDALPPLLAGFAAYFVNTFVTYWWHRarhandtLWRLFHQLHHAPQRIEVF 115
Cdd:TIGR04249 218 TDEVSPLNQAFAAAFLNDAALQFWHR-------YANFLEETLRKKLRIMIF 261
 
Name Accession Description Interval E-value
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
 73-226 5.65e-38

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 130.44  E-value: 5.65e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489193357  73 AGFAAYFVNTFVTYWWHRARHANDTLWRlFHQLHHAPQRIEVFTSFYKHPTEMVFNSLLGsFVAYVVMGISIEAGAYYIM 152
Cdd:COG3000    1 QFLLAFLLLDFVYYWLHRLSHRVPLLWR-FHAVHHSSERMNLLTALRFHPLEILLSALLF-LLPLALLGFPPEAVLLYLA 78

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489193357 153 FAALGEMFYHSNLRTP--HVLGYLFQRPEMHRIHHQRDRHE--CNYS-DFPIWDMLFGTYENPRRIDEPQGFAGDKEQQ 226
Cdd:COG3000   79 LNLLYQFFNHSNIRLPldGPLEYVFVTPSHHRVHHSRNPEYldKNYGgILSIWDRLFGTYRPEEREPIRYGLTGPPRPR 157
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 75-206 3.81e-26

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 99.04  E-value: 3.81e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489193357   75 FAAYFVNTFVTYWWHRARHANDTLWRLFHQLHHAPQRIEVFTSFYKHPTEMVFNSLLGsFVAYVVMGISIEAGAYYIMFA 154
Cdd:pfam04116   2 LLGLLLFDFLFYWVHRLLHRLPWLWRRFHKVHHSSEAPNALTALRFHPLEALLFALLV-LLPLLLLGLPLLAFLLGLLLG 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 489193357  155 ALGEMFYHSN--LRTPHVLGYLFQRPEMHRIHHQRDrHECNYSD-FPIWDMLFGT 206
Cdd:pfam04116  81 TLWYLFIHSGllFPLPWLLKRLLGTPRFHRLHHSKN-EEYNFGVtFPLWDRLFGT 134
PLN02869 PLN02869
fatty aldehyde decarbonylase
 83-206 3.82e-05

fatty aldehyde decarbonylase


Pssm-ID: 166510 [Multi-domain]  Cd Length: 620  Bit Score: 44.45  E-value: 3.82e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489193357  83 FVTYWWHRARHaNDTLWRLFHQLHHAPQRIEVFTSFYkHPtemvFNSLLGSFVAY-------VVMGI-SIEAGAYYIMFA 154
Cdd:PLN02869 140 FLYYWLHRALH-HHYLYSRYHSHHHSSIVTEPITSVI-HP----FAEHIAYFLLFaipllttIFTGTaSIAAFFGYISYI 213

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 489193357 155 ALGEMFYHSNLR-TPHV-------LGYLFQRPEMHRIHHQRDRheCNYSDF-PIWDMLFGT 206
Cdd:PLN02869 214 DFMNNMGHCNFElIPKWlfsifppLKYLMYTPSYHSLHHTQFR--TNYSLFmPIYDYIYGT 272
SCM_chp_ScmC TIGR04249
SynChlorMet cassette protein ScmC; A biosynthesis cassette found in Syntrophobacter ...
 65-115 4.29e-03

SynChlorMet cassette protein ScmC; A biosynthesis cassette found in Syntrophobacter fumaroxidans MPOB, Chlorobium limicola DSM 245, Methanocella paludicola SANAE, and delta proteobacterium NaphS2 contains two PqqE-like radical SAM/SPASM domain proteins, a PqqD homolog, and a conserved hypothetical protein. These components suggest modification of a ribosomally produced peptide precursor, but the precursor has not been identified. Members of this family are designated ScmC.


Pssm-ID: 275080  Cd Length: 292  Bit Score: 37.58  E-value: 4.29e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 489193357   65 SDALPPLLAGFAAYFVNTFVTYWWHRarhandtLWRLFHQLHHAPQRIEVF 115
Cdd:TIGR04249 218 TDEVSPLNQAFAAAFLNDAALQFWHR-------YANFLEETLRKKLRIMIF 261
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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