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Conserved domains on  [gi|489025397|ref|WP_002935808|]
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MULTISPECIES: tRNA (cytidine(34)-2'-O)-methyltransferase [Campylobacter]

Protein Classification

tRNA (cytidine(34)-2'-O)-methyltransferase( domain architecture ID 10000715)

tRNA (cytidine(34)-2'-O)-methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine to the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA)

EC:  2.1.1.207
Gene Ontology:  GO:0003723|GO:0008173|GO:1904047|GO:0141098|GO:0001510
SCOP:  4002340

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-149 7.81e-80

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


:

Pssm-ID: 439989  Cd Length: 153  Bit Score: 232.62  E-value: 7.81e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397   1 MFNIVLVHPRIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIWENLEEFLNENMiyKNHFFFA 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALP--GGRLFLF 78

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489025397  81 TTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNWENAITIPMKSYGRSLNLATSVGIISYEALRQN 149
Cdd:COG0219   79 TTKGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAAPADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQL 147
 
Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-149 7.81e-80

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 232.62  E-value: 7.81e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397   1 MFNIVLVHPRIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIWENLEEFLNENMiyKNHFFFA 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALP--GGRLFLF 78

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489025397  81 TTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNWENAITIPMKSYGRSLNLATSVGIISYEALRQN 149
Cdd:COG0219   79 TTKGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAAPADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQL 147
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 3-149 1.16e-73

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 216.94  E-value: 1.16e-73
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397   3 NIVLVHPRIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIWENLEEFLNENMIYKnhFFFATT 82
Cdd:cd18094    1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALPPGR--LFLFTT 78

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 489025397  83 KSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNWENAITIPMKSYGRSLNLATSVGIISYEALRQN 149
Cdd:cd18094   79 KGTKPYTDFDFKPGDYLVFGRESTGLPEEILDAFPDRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 1-149 1.93e-47

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 150.65  E-value: 1.93e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397    1 MFNIVLVHPRIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIWENLEEFLNENMiyKNHFFFA 80
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEK--PQKLFAL 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489025397   81 TTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNWENAITIPMKSYGRSLNLATSVGIISYEALRQN 149
Cdd:TIGR00185  79 TKKGTPAHSAVTYQDGDYLMFGPETRGLPQSILDNLMEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQL 147
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 1-143 1.83e-41

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 135.35  E-value: 1.83e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397    1 MFNIVLVHPRIPQNTGSIGRMCFNAGF-KLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIWENLEEFLNENmiyKNHFF- 78
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGAdGLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDL---KEAGYa 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 489025397   79 -FATTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNwENAITIPMKSYGRSLNLATSVGIISY 143
Cdd:pfam00588  78 vVATSLGGKSLYEIDFPKKVALVFGNEGTGLSNEVLELC-DRLVRIPMSGDVESLNVAVAAAIALY 142
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-148 3.52e-36

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 122.41  E-value: 3.52e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397   1 MFNIVLVHPRIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIWENLEEFL-NENmiyKNHFFF 79
Cdd:PRK10358   1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLeAEN---PQRLFA 77

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397  80 ATTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQ-LNWENAITIPMKSYGRSLNLATSVGIISYEALRQ 148
Cdd:PRK10358  78 LTTKGTPAHSAVSYQDGDYLMFGPETRGLPASILDaLPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQ 147
 
Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-149 7.81e-80

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 232.62  E-value: 7.81e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397   1 MFNIVLVHPRIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIWENLEEFLNENMiyKNHFFFA 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALP--GGRLFLF 78

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489025397  81 TTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNWENAITIPMKSYGRSLNLATSVGIISYEALRQN 149
Cdd:COG0219   79 TTKGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAAPADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQL 147
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 3-149 1.16e-73

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 216.94  E-value: 1.16e-73
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397   3 NIVLVHPRIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIWENLEEFLNENMIYKnhFFFATT 82
Cdd:cd18094    1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALPPGR--LFLFTT 78

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 489025397  83 KSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNWENAITIPMKSYGRSLNLATSVGIISYEALRQN 149
Cdd:cd18094   79 KGTKPYTDFDFKPGDYLVFGRESTGLPEEILDAFPDRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 1-149 1.93e-47

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 150.65  E-value: 1.93e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397    1 MFNIVLVHPRIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIWENLEEFLNENMiyKNHFFFA 80
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEK--PQKLFAL 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489025397   81 TTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNWENAITIPMKSYGRSLNLATSVGIISYEALRQN 149
Cdd:TIGR00185  79 TKKGTPAHSAVTYQDGDYLMFGPETRGLPQSILDNLMEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQL 147
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 1-143 1.83e-41

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 135.35  E-value: 1.83e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397    1 MFNIVLVHPRIPQNTGSIGRMCFNAGF-KLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIWENLEEFLNENmiyKNHFF- 78
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGAdGLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDL---KEAGYa 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 489025397   79 -FATTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNwENAITIPMKSYGRSLNLATSVGIISY 143
Cdd:pfam00588  78 vVATSLGGKSLYEIDFPKKVALVFGNEGTGLSNEVLELC-DRLVRIPMSGDVESLNVAVAAAIALY 142
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-148 3.52e-36

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 122.41  E-value: 3.52e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397   1 MFNIVLVHPRIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIWENLEEFL-NENmiyKNHFFF 79
Cdd:PRK10358   1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLeAEN---PQRLFA 77

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397  80 ATTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQ-LNWENAITIPMKSYGRSLNLATSVGIISYEALRQ 148
Cdd:PRK10358  78 LTTKGTPAHSAVSYQDGDYLMFGPETRGLPASILDaLPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQ 147
SpoU-like_family cd18082
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 4-145 2.62e-23

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349955  Cd Length: 145  Bit Score: 88.82  E-value: 2.62e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397   4 IVLVHPRIPQNTGSIGRMCFNAG-FKLHIVKPTVFDISQKAVRrAGLDYWDKLEPIIWENLEEFLNENMIYKNHFF-FAT 81
Cdd:cd18082    2 LVLDNIRSPGNIGSIFRTADAFGvEKIYLVGPTADIFDPKAKR-TSRGTHFSVPYERVDNFEEYLKSLKEEGYPLIgLEL 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 489025397  82 TKSQKAYFDAEF--QKNDFLFFGSESYGLPMELMQLNwENAITIPMKSYGRSLNLATSVGIISYEA 145
Cdd:cd18082   81 TDGSIPLSEFKFkkPKKIALIFGNERSGLSPELLALC-DYTVHIPMSGKNSSLNVSVATGIALYEF 145
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 2-144 2.24e-18

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 76.38  E-value: 2.24e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397   2 FNIVLVHPRIPQNTGSIGRMCFNAGFK-LHIVKPTVFDISQKAVRRA--GLDYWDKLEpiIWENLEEFLNENmiyknHFF 78
Cdd:cd18093    1 IRVVLVEPQHPGNIGAVARAMKNFGLSdLRLVNPRCDDPEEAARALAsgAEDVLENAK--VYDTLEEALADC-----DLV 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397  79 FATT----KSQKAYFDAEFQKNDF----------LFFGSESYGLPMElmQLNWENAI-TIPMKSYGRSLNLATSVGIISY 143
Cdd:cd18093   74 VATTarprGLRRPLLTPRELAELLkehaaggkvaLVFGREDSGLTNE--ELALCDLLvTIPTNPEYPSLNLAQAVAIVLY 151


                 .
gi 489025397 144 E 144
Cdd:cd18093  152 E 152
SpoU-like_RNA-MTase cd18104
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 2-149 3.44e-18

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349977  Cd Length: 146  Bit Score: 75.69  E-value: 3.44e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397   2 FNIVLVHPRIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIWENLE--EFLNENMIyknHFFF 79
Cdd:cd18104    1 LILVLEGLEKPGNLGAILRSADAAGADAVILCDPKTDLFNPNVIRASLGAVFTLPVAEASSEEtlAWLKENKI---RILA 77

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397  80 ATTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNWENaITIPMKSYGRSLNLATSVGIISYEALRQN 149
Cdd:cd18104   78 TTPKAEKLYYEADLRGPIAIVVGSESEGLSEFWLKAADER-VRIPMAGQADSLNVSVAAAILLYEALRQR 146
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 12-145 2.31e-12

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 60.52  E-value: 2.31e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397  12 PQNTGSIGRMCFNAGFKLHIV-KPTVFDISQKAVRRA-GldYWDKLEPIIWENLEEFLNEnMIYKNHFFFATT-KSQKAY 88
Cdd:cd18095   11 PGNLGTIIRTAAAFGVDGVILsKGTVDPYNPKVVRASmG--ALFRLPIIRVDDLEELLEE-LKEAGYKVVAADlDGGSSL 87

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 489025397  89 FDAEFQKNDFLFFGSESYGLPMELMQLNWENaITIPMksYGR--SLNLATSVGIISYEA 145
Cdd:cd18095   88 YDLDLPKPVALVLGNEGNGLSDELLELCDKR-VTIPM--SGGveSLNVAVAAAILLYEL 143
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 4-154 2.31e-11

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 59.36  E-value: 2.31e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397   4 IVLVHPRIPQNTGSIGRMCFNAGF-KLHIVKPTVFDiSQKAVRRA--GLDYWDKLEpiIWENLEEFLNENmiyknHFFFA 80
Cdd:COG0565    6 VVLVEPQHPGNIGAAARAMKNFGLsDLRLVNPRGWP-DEEARAMAsgADDVLENAR--VVDTLEEALADC-----DLVVG 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397  81 TT----KSQKAYFDAEfqknDF--------------LFFGSESYGLPM-ELMQLNWenAITIPM-KSYGrSLNLATSVGI 140
Cdd:COG0565   78 TTarprELRKPVLTPR----EAaeellaraaggkvaLVFGRERSGLTNeELALCDA--LVTIPTnPEYP-SLNLAQAVQV 150

                        170
                 ....*....|....
gi 489025397 141 ISYEaLRQNFRNFA 154
Cdd:COG0565  151 VAYE-LRKAALEAP 163
SpoU-like_RNA-MTase cd18109
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 57-144 5.02e-10

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349982  Cd Length: 141  Bit Score: 54.47  E-value: 5.02e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397  57 PIIWENLEEFLNENMiyKNHFFFATTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNwENAITIPmkSYGR---SLN 133
Cdd:cd18109   55 QIHYTDLPALLAELK--KAFPVYGADLDGENLYEFTLPEKGILVMGNEGHGISPEVLKLL-THRLTIP--RYGKgaeSLN 129

                         90
                 ....*....|.
gi 489025397 134 LATSVGIISYE 144
Cdd:cd18109  130 VAVATAILLSE 140
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 4-149 8.24e-10

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 55.59  E-value: 8.24e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397   4 IVLVHPRIPQNTGSIGRMCFNAGFKlHIV--KPTVFDISQKAVR-RAGLdywdkLE--PII-WENLEEFLNEnmiYKNHF 77
Cdd:COG0566  118 LVLDGVQDPGNLGAILRSAAAFGVD-GVIlpKRRADPYNPKVVRaSAGA-----LFhvPVArVTNLADALEE---LKEAG 188

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397  78 F--FATT-KSQKAYFDAEFQKNDFLFFGSESYGLPMELMqlnwENA---ITIPMksYGR--SLNLATSVGIISYEALRQN 149
Cdd:COG0566  189 FwvVGTDlDGEASLYEVDLPGPVALVLGNEGKGLSRLVL----EACdelVRIPM--AGGveSLNVAVAAAILLYEAARQR 262
SpoU-like_RNMTL1 cd18106
SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) ...
 10-145 1.93e-08

SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) catalyzes the methylation of 2'-O-ribose residues G1370 to GmG residue of the mitochondrial 16S rRNA. RNMTL1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349979  Cd Length: 148  Bit Score: 50.33  E-value: 1.93e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397  10 RIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLePIIwENLE-EFLNENMIYKNHFFFATTKSQ-KA 87
Cdd:cd18106    9 RDPGNLGSILRSAAAAGCSKVLLTKGCVDPWEPKVLRAGAGAHFRL-PIY-GNLSwDDIPNYLPDDSKVFLADNNLPvQP 86

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 489025397  88 YFDAEFQ-KNDFLFFGSESYGLPMELMQLNWEN---AITIPMKSYGRSLNLATSVGIISYEA 145
Cdd:cd18106   87 YDSIDYCsGPIVLVIGGETHGVSPEAYKLAHSTggsRVHIPLANGVDSLNSAVAASILLFEM 148
rRNA_methyl_1 TIGR00050
RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of ...
 1-151 7.01e-08

RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases, and is now characterized, in E. coli, as a tRNA:Cm32/Um32 methyltransferase. It may be named TrMet(Xm32), or TrmJ, according to the nomenclature style chosen [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272876 [Multi-domain]  Cd Length: 233  Bit Score: 49.71  E-value: 7.01e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397    1 MFNIVLVHPRIPQNTGSIGRMCFNAGFK-LHIVKPTVfDISQKAvrragldYW------DKLEPI-IWENLEEFLNE-NM 71
Cdd:TIGR00050   3 NIRIVLVEPSHPGNIGSIARAMKNMGLTeLRLVNPRV-KLEEEA-------YAlaagarDILDNAkVVDTLDEALDDcDF 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397   72 I------YKNHFFFATTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNwENAITIPMKSYGRSLNLATSVGIISYEa 145
Cdd:TIGR00050  75 VvgtsarSRNLQRPMLTPRELAPKLVAEKGKVALVFGREDSGLTNEELQKC-HVLVSIPTSEEYPSLNLSHAVAVILYE- 152


                  ....*.
gi 489025397  146 LRQNFR 151
Cdd:TIGR00050 153 LRMAWL 158
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 12-148 9.79e-07

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 45.92  E-value: 9.79e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397  12 PQNTGSIGRMCFNAGF-KLHIVKP-TVFDISQKAVRRAglDYWDKLEpiIWENLEEFLNEnmiYKNHFF--FATTKSQKA 87
Cdd:cd18092   27 PHNASAVLRTCDAFGVqDVHVIEPkNKFKKNRGVAMGA--DKWLDVH--RHDSTEECLAA---LKEQGYriVATSLSPDA 99

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489025397  88 --YFDAEFQKNDFLFFGSESYGLPMELMQLNwENAITIPMksYG--RSLNLATSVGIISYEALRQ 148
Cdd:cd18092  100 vpLDELDYTRPTALVFGNEKDGVSEEALAAA-DERVYIPM--YGfvESLNVSVAAAIILYELQRQ 161
SpoU-like cd18097
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 81-145 3.17e-06

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349970  Cd Length: 144  Bit Score: 44.09  E-value: 3.17e-06
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489025397  81 TTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNwENAITIPMKSYGRSLNLATSVGIISYEA 145
Cdd:cd18097   81 TTENSVPLHEARLPRPLALVLGNEVRGVSPEVLALC-DAVVEIPMFGRKNSLNVAVAAAVALYEL 144
SpoU-like_RlmB cd18103
SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the ...
 12-145 6.13e-06

SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349976  Cd Length: 143  Bit Score: 43.14  E-value: 6.13e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397  12 PQNTGSIGRMCFNAGFKLHIVK--------PTVFDISQKAVrragldywDKLePIIW-ENLEEFLNEnmIYKNHFFF--A 80
Cdd:cd18103   11 PHNLGAIIRSAEAFGVDGVIIPkrrsapltPTVAKASAGAL--------EHL-PIARvTNLARALEE--LKEAGFWIygA 79

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489025397  81 TTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMqlnwENA---ITIPMKSYGRSLNLATSVGIISYEA 145
Cdd:cd18103   80 DGEAEKSLYDVDLTGPLALVLGSEGKGLRRLVL----KNCdflVKIPMAGKVDSLNVSVAAGIILYEI 143
rRNA_methyl_3 TIGR00186
rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases ...
 12-148 3.48e-05

rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 129290 [Multi-domain]  Cd Length: 237  Bit Score: 42.33  E-value: 3.48e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397   12 PQNTGSIGRMCFNAGFKLHIVK--------PTVFDISQKAVrragldywdKLEPIIW-ENLEEFLNEnmiYKNHFFFATT 82
Cdd:TIGR00186 101 PHNLGAILRTAEAFGVDGVILPkrrsaplnSTVVKTSSGAV---------EYVPLARvTNLSRTITK---LKESGFWTVG 168

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397   83 KSQKAYfDAEFQKNDF----LFFGSESYGLPmELMQLNWENAITIPMKSYGRSLNLATSVGIISYEALRQ 148
Cdd:TIGR00186 169 TDLDAQ-DTLYQVKLTkplaLVVGNEGEGVS-RLIKENCDFLIKIPMAGKVDSLNVSVAAGILLFEIKRQ 236
PRK10864 PRK10864
putative methyltransferase; Provisional
 12-149 3.60e-04

putative methyltransferase; Provisional


Pssm-ID: 236779 [Multi-domain]  Cd Length: 346  Bit Score: 39.38  E-value: 3.60e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489025397  12 PQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRA--GLDYwdkLEPIIWENLEEFLNEnmIYKNHFFFATTKSQK--A 87
Cdd:PRK10864 209 PHNLGGIMRSCAHFGVKGVVVQDAALLESGAAIRTAegGAEH---VQPITGDSFVDVLDD--FRQAGYTIVTTSSHKgtP 283

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489025397  88 YFDAEFQKNDFLFFGSESYGLPmelmQLNWENA---ITIPMKSYGRSLNLATSVGIISYEALRQN 149
Cdd:PRK10864 284 LFKASLPAKMVLVLGQEYDGLS----DAARQQGdlsVSIDGTGNVESLNVSVATGVLLAEWWRQN 344
SpoU-like_TRM3-like cd18091
SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O) ...
 81-144 1.10e-03

SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O)-methyltransferase TRM3 catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in various tRNAs. TRM3 is similar to C-terminal domain of TAR (HIV-1) RNA binding protein 1 (TARBP1), a protein binding to TAR, which functions as a RNA regulatory signal by forming a stable stem-loop structure to which transactivator protein Tat binds. The role of TARBP1 is believed to be to disengage RNA polymerase II from TAR during transcriptional elongation. TRM3 and the C-terminal methyltransferase domain of TARBP1 are members of the SPOUT methyltransferase superfamily.


Pssm-ID: 349964  Cd Length: 145  Bit Score: 37.12  E-value: 1.10e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 489025397  81 TTKSQKAYfDAEFQKNDFLFFGSESYGLPMELMQLnWENAITIPMKSYGRSLNLATSVGIISYE 144
Cdd:cd18091   83 TANSVSLH-DFQFPKKTVLVLGNEKEGIPAELLSL-LDVCVEIPQLGVIRSLNVHVSGAIAIYE 144
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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