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Conserved domains on  [gi|489005889|ref|WP_002916479|]
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MULTISPECIES: glycine cleavage system protein GcvH [Enterobacteriaceae]

Protein Classification

glycine cleavage system protein H( domain architecture ID 10785558)

glycine cleavage system protein H plays a role in the degradation of glycine by shuttling the methylamine group of glycine from P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase).

EC:  1.4.1.27
Gene Ontology:  GO:0019464|GO:0009249|GO:0005960
PubMed:  20375021|18941301

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 2-129 1.79e-76

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


:

Pssm-ID: 440275  Cd Length: 128  Bit Score: 222.31  E-value: 1.79e-76
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489005889   2 SNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVAV 81
Cdd:COG0509    1 MEIPDDLKYTEDHEWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEV 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 489005889  82 NEELNDSPELVNSDPYTDGWIFKIKASDEAQVAALLDAAAYEALLEDE 129
Cdd:COG0509   81 NEALEDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEEE 128
 
Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 2-129 1.79e-76

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 222.31  E-value: 1.79e-76
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489005889   2 SNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVAV 81
Cdd:COG0509    1 MEIPDDLKYTEDHEWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEV 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 489005889  82 NEELNDSPELVNSDPYTDGWIFKIKASDEAQVAALLDAAAYEALLEDE 129
Cdd:COG0509   81 NEALEDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEEE 128
PRK01202 PRK01202
glycine cleavage system protein GcvH;
1-128 7.60e-74

glycine cleavage system protein GcvH;


Pssm-ID: 234918  Cd Length: 127  Bit Score: 215.41  E-value: 7.60e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489005889   1 MSNVPAELKYSKEHEWLRKEaDGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVA 80
Cdd:PRK01202   1 MMNIPADLKYTKSHEWVRVE-GDTATVGITDHAQEQLGDIVFVELPEVGDEVKAGETFGVVESVKAASDIYAPVSGEVVE 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 489005889  81 VNEELNDSPELVNSDPYTDGWIFKIKASDEAQVAALLDAAAYEALLED 128
Cdd:PRK01202  80 VNEALEDSPELVNEDPYGEGWLFKIKPSDESELDDLLDAEAYEALIEE 127
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
 3-129 9.69e-61

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 182.73  E-value: 9.69e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489005889    3 NVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVAVN 82
Cdd:TIGR00527   1 QGPQDLRYSSEHEWVRVEGDKTATVGITKFAQDELGDIVFVELPEVGAEVAAGESLGSVESVKAASDIYAPVDGTVVEVN 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 489005889   83 EELNDSPELVNSDPYTDGWIFKIKASD-EAQVAALLDAAAYEALLEDE 129
Cdd:TIGR00527  81 TALEDSPELVNEDPYGDGWLIKVKLSDgESEVEGLMSAEQYEATLEHD 128
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 8-129 6.49e-56

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 170.21  E-value: 6.49e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489005889    8 LKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVAVNEELND 87
Cdd:pfam01597   1 LYYTKEHEWVKVEGDGTATVGITDFAQAQLGDIVFVELPEVGTKVKKGESLAAIESVKAASPIYAPVSGEVVEVNEKLED 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 489005889   88 SPELVNSDPYTDGWIFKIKASDEAQVAALLDAAAYEALLEDE 129
Cdd:pfam01597  81 NPGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTLKEI 122
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 10-105 1.08e-51

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 158.47  E-value: 1.08e-51
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489005889  10 YSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVAVNEELNDSP 89
Cdd:cd06848    1 YTKDHEWVKVEGDGIATVGITDYAQDLLGDIVFVELPEVGTEVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDNP 80

                         90
                 ....*....|....*.
gi 489005889  90 ELVNSDPYTDGWIFKI 105
Cdd:cd06848   81 ELINSDPYGEGWLVKI 96
 
Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 2-129 1.79e-76

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 222.31  E-value: 1.79e-76
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489005889   2 SNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVAV 81
Cdd:COG0509    1 MEIPDDLKYTEDHEWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEV 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 489005889  82 NEELNDSPELVNSDPYTDGWIFKIKASDEAQVAALLDAAAYEALLEDE 129
Cdd:COG0509   81 NEALEDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEEE 128
PRK01202 PRK01202
glycine cleavage system protein GcvH;
1-128 7.60e-74

glycine cleavage system protein GcvH;


Pssm-ID: 234918  Cd Length: 127  Bit Score: 215.41  E-value: 7.60e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489005889   1 MSNVPAELKYSKEHEWLRKEaDGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVA 80
Cdd:PRK01202   1 MMNIPADLKYTKSHEWVRVE-GDTATVGITDHAQEQLGDIVFVELPEVGDEVKAGETFGVVESVKAASDIYAPVSGEVVE 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 489005889  81 VNEELNDSPELVNSDPYTDGWIFKIKASDEAQVAALLDAAAYEALLED 128
Cdd:PRK01202  80 VNEALEDSPELVNEDPYGEGWLFKIKPSDESELDDLLDAEAYEALIEE 127
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
 3-129 9.69e-61

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 182.73  E-value: 9.69e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489005889    3 NVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVAVN 82
Cdd:TIGR00527   1 QGPQDLRYSSEHEWVRVEGDKTATVGITKFAQDELGDIVFVELPEVGAEVAAGESLGSVESVKAASDIYAPVDGTVVEVN 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 489005889   83 EELNDSPELVNSDPYTDGWIFKIKASD-EAQVAALLDAAAYEALLEDE 129
Cdd:TIGR00527  81 TALEDSPELVNEDPYGDGWLIKVKLSDgESEVEGLMSAEQYEATLEHD 128
PRK13380 PRK13380
glycine cleavage system protein H; Provisional
 1-126 1.08e-57

glycine cleavage system protein H; Provisional


Pssm-ID: 237370  Cd Length: 144  Bit Score: 175.19  E-value: 1.08e-57
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489005889   1 MSNVPAELKYSKE-HEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIV 79
Cdd:PRK13380   6 GCEIPSELRYTIKnHEWLRLEGDGTVTVGITDYAQTMAGDVVFVRLKELGKKVEKGKPVATLESGKWAGPVPAPLTGEVV 85

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 489005889  80 AVNEELNDSPELVNSDPYTDGWIFKIKASDEAQVAALLDAAAYEALL 126
Cdd:PRK13380  86 EVNEALEDSPELVNEDPYGEGWFFRFKPANPEELKQLLDGDAADRLL 132
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 8-129 6.49e-56

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 170.21  E-value: 6.49e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489005889    8 LKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVAVNEELND 87
Cdd:pfam01597   1 LYYTKEHEWVKVEGDGTATVGITDFAQAQLGDIVFVELPEVGTKVKKGESLAAIESVKAASPIYAPVSGEVVEVNEKLED 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 489005889   88 SPELVNSDPYTDGWIFKIKASDEAQVAALLDAAAYEALLEDE 129
Cdd:pfam01597  81 NPGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTLKEI 122
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 10-105 1.08e-51

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 158.47  E-value: 1.08e-51
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489005889  10 YSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVAVNEELNDSP 89
Cdd:cd06848    1 YTKDHEWVKVEGDGIATVGITDYAQDLLGDIVFVELPEVGTEVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDNP 80

                         90
                 ....*....|....*.
gi 489005889  90 ELVNSDPYTDGWIFKI 105
Cdd:cd06848   81 ELINSDPYGEGWLVKI 96
PRK00624 PRK00624
glycine cleavage system protein H; Provisional
 10-110 6.26e-18

glycine cleavage system protein H; Provisional


Pssm-ID: 167014  Cd Length: 114  Bit Score: 73.33  E-value: 6.26e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489005889  10 YSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVAVNEELNDSP 89
Cdd:PRK00624   4 YSDYHVWIEPIHSRIVRLGLTSKMQENLGNILHIDLPSVGSFCKEGEVLVILESSKSAIEVLSPVSGEVIEVNTALEDDI 83

                         90       100
                 ....*....|....*....|.
gi 489005889  90 ELVNSDPYTDGWIFKIKASDE 110
Cdd:PRK00624  84 QPINNAPESEGWFVVVQLDED 104
Biotinyl_lipoyl_domains cd06663
Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the ...
 26-105 1.56e-12

Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the dihydrolipoyl acyltransferase component (E2) of 2-oxo acid dehydrogenases, and the H-protein of the glycine cleavage system (GCS). These domains transport CO2, acyl, or methylamine, respectively, between components of the complex/protein via a biotinyl or lipoyl group, which is covalently attached to a highly conserved lysine residue.


Pssm-ID: 133456 [Multi-domain]  Cd Length: 73  Bit Score: 58.22  E-value: 1.56e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489005889  26 TVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVAVNEELNDSPELvnsdpytDGWIFKI 105
Cdd:cd06663    1 TILIPDLAQHLGDGTVVKWLKKVGDKVKKGDVLAEIEAMKATSDVEAPKSGTVKKVLVKEGTKVEG-------DTPLVKI 73
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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