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Conserved domains on  [gi|488409238|ref|WP_002478623|]
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MULTISPECIES: NAD(P)H-dependent oxidoreductase [Staphylococcus]

Protein Classification

NAD(P)H-dependent oxidoreductase( domain architecture ID 10114874)

NAD(P)H-dependent oxidoreductase, similar to nitroreductase NfsB, which catalyzes the reduction of flavin or nitrocompounds using NAD(P)H as electron donor

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 7-214 1.29e-71

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


:

Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 214.81  E-value: 1.29e-71
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238   7 TIINALNFRHATKIFDSSKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKPYSWGAQKQLDTASRFVVIL 86
Cdd:cd02149    1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAAWFNQPQIKDASHVVVFL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  87 ARKnvtaqsdyvqhilrdikkydthtipaveqkfndfqtnfhindsertllDWASKQTYIALANMMTSAALLGIDSCPME 166
Cdd:cd02149   81 AKK------------------------------------------------DWSAKQTYIALGNMLLAAAMLGIDSCPIE 112

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 488409238 167 GFDLDKVTEILqdegVIDTVHFAPSVMVAFGYRKDEPKGKVRQPATDV 214
Cdd:cd02149  113 GFDPAKLDEIL----GLDEKGYKISVMVAFGYRSEEKLPKSRKPLEDV 156
 
Name Accession Description Interval E-value
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 7-214 1.29e-71

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 214.81  E-value: 1.29e-71
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238   7 TIINALNFRHATKIFDSSKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKPYSWGAQKQLDTASRFVVIL 86
Cdd:cd02149    1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAAWFNQPQIKDASHVVVFL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  87 ARKnvtaqsdyvqhilrdikkydthtipaveqkfndfqtnfhindsertllDWASKQTYIALANMMTSAALLGIDSCPME 166
Cdd:cd02149   81 AKK------------------------------------------------DWSAKQTYIALGNMLLAAAMLGIDSCPIE 112

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 488409238 167 GFDLDKVTEILqdegVIDTVHFAPSVMVAFGYRKDEPKGKVRQPATDV 214
Cdd:cd02149  113 GFDPAKLDEIL----GLDEKGYKISVMVAFGYRSEEKLPKSRKPLEDV 156
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 8-215 4.44e-41

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 137.29  E-value: 4.44e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238   8 IINALNFRHATKIFDSsKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKP-YSWGAQKQLDTASRFVVIL 86
Cdd:COG0778    1 LLELLLTRRSVRKFTD-KPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEaLAEANQEWVADAPVLIVVC 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  87 ARKnvtaqsdyvqhilrdikkydthtipaveqkfndfqtnfhiNDSERTLLDWASKQTYIALANMMTSAALLGIDSCPME 166
Cdd:COG0778   80 ADP----------------------------------------DRSEKVPERYALLDAGIAAQNLLLAARALGLGTCWIG 119

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*....
gi 488409238 167 GFDLDKVTEILQdegvIDTvHFAPSVMVAFGYRKDEPKGKVRQPATDVI 215
Cdd:COG0778  120 GFDPEKVRELLG----LPE-GEEPVALLALGYPAEELNPRPRKPLEEVV 163
PRK11053 PRK11053
oxygen-insensitive NAD(P)H nitroreductase;
4-202 7.92e-34

oxygen-insensitive NAD(P)H nitroreductase;


Pssm-ID: 182929 [Multi-domain]  Cd Length: 217  Bit Score: 120.46  E-value: 7.92e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238   4 MNetIINALNFRHATKIFDSSKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKP-----YSWGAQKQLDt 78
Cdd:PRK11053   1 MD--IVSVAKKRYTTKAFDPSKKLPAEQIEQIKTLLRFSPSSVNSQPWHFIVASTEEGKARIAKaaagnYAFNERKILD- 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  79 ASRFVVILARKNVTAqsDYVQHIL----RDiKKYDTHTIPAVEQKFNDFQTNFHINDsERTLLDWASKQTYIALANMMTS 154
Cdd:PRK11053  78 ASHVVVFCAKTDMDD--AYLELVLeqedAD-GRFATEEAKAAQDKGRRFFADMHRKE-LKDLQHWMEKQVYLALGNLLLG 153

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 488409238 155 AALLGIDSCPMEGFDLDKVTEI--LQDEGvidtvhFAPSVMVAFGYRKDE 202
Cdd:PRK11053 154 AAALGIDATPIEGFDAAILDAEfgLREKG------LTSSVVVPLGYHSEE 197
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 15-198 3.53e-29

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 107.09  E-value: 3.53e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238   15 RHATKIFDSSKqVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKPYSWGAQKQLDTASRFVVilarknvtaq 94
Cdd:pfam00881   4 RRSVRKFDPEP-VPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELLLVEPAAALLLL---------- 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238   95 sDYVQHILRDIKKYDTHTIPAVeqKFNDFQTNFHINDSERTLLDWASKQTYIALANMMTSAALLGIDSCPMEGFDLDKVT 174
Cdd:pfam00881  73 -LRRDANLKLLLQDFLRGAPVL--IVITASLSTYLRKAAERAYREALLDAGAAAQNLLLAATSLGLGSCPIGGFDAAAVR 149

                         170       180
                  ....*....|....*....|....
gi 488409238  175 EILQdegvIDTVHFaPSVMVAFGY 198
Cdd:pfam00881 150 ELLG----LPDDER-LVGLIAVGY 168
 
Name Accession Description Interval E-value
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 7-214 1.29e-71

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 214.81  E-value: 1.29e-71
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238   7 TIINALNFRHATKIFDSSKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKPYSWGAQKQLDTASRFVVIL 86
Cdd:cd02149    1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAAWFNQPQIKDASHVVVFL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  87 ARKnvtaqsdyvqhilrdikkydthtipaveqkfndfqtnfhindsertllDWASKQTYIALANMMTSAALLGIDSCPME 166
Cdd:cd02149   81 AKK------------------------------------------------DWSAKQTYIALGNMLLAAAMLGIDSCPIE 112

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 488409238 167 GFDLDKVTEILqdegVIDTVHFAPSVMVAFGYRKDEPKGKVRQPATDV 214
Cdd:cd02149  113 GFDPAKLDEIL----GLDEKGYKISVMVAFGYRSEEKLPKSRKPLEDV 156
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 8-215 4.44e-41

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 137.29  E-value: 4.44e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238   8 IINALNFRHATKIFDSsKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKP-YSWGAQKQLDTASRFVVIL 86
Cdd:COG0778    1 LLELLLTRRSVRKFTD-KPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEaLAEANQEWVADAPVLIVVC 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  87 ARKnvtaqsdyvqhilrdikkydthtipaveqkfndfqtnfhiNDSERTLLDWASKQTYIALANMMTSAALLGIDSCPME 166
Cdd:COG0778   80 ADP----------------------------------------DRSEKVPERYALLDAGIAAQNLLLAARALGLGTCWIG 119

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*....
gi 488409238 167 GFDLDKVTEILQdegvIDTvHFAPSVMVAFGYRKDEPKGKVRQPATDVI 215
Cdd:COG0778  120 GFDPEKVRELLG----LPE-GEEPVALLALGYPAEELNPRPRKPLEEVV 163
PRK11053 PRK11053
oxygen-insensitive NAD(P)H nitroreductase;
4-202 7.92e-34

oxygen-insensitive NAD(P)H nitroreductase;


Pssm-ID: 182929 [Multi-domain]  Cd Length: 217  Bit Score: 120.46  E-value: 7.92e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238   4 MNetIINALNFRHATKIFDSSKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKP-----YSWGAQKQLDt 78
Cdd:PRK11053   1 MD--IVSVAKKRYTTKAFDPSKKLPAEQIEQIKTLLRFSPSSVNSQPWHFIVASTEEGKARIAKaaagnYAFNERKILD- 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  79 ASRFVVILARKNVTAqsDYVQHIL----RDiKKYDTHTIPAVEQKFNDFQTNFHINDsERTLLDWASKQTYIALANMMTS 154
Cdd:PRK11053  78 ASHVVVFCAKTDMDD--AYLELVLeqedAD-GRFATEEAKAAQDKGRRFFADMHRKE-LKDLQHWMEKQVYLALGNLLLG 153

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 488409238 155 AALLGIDSCPMEGFDLDKVTEI--LQDEGvidtvhFAPSVMVAFGYRKDE 202
Cdd:PRK11053 154 AAALGIDATPIEGFDAAILDAEfgLREKG------LTSSVVVPLGYHSEE 197
MhqN-like cd02137
nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily ...
 10-214 4.70e-33

nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily of the nitroreductase family containing uncharacterized proteins; includes nitroreductases MhqN, YodC, YdgI, DrgA. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380314 [Multi-domain]  Cd Length: 147  Bit Score: 116.19  E-value: 4.70e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  10 NALNFRHATKIFDSSKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKPYSWGaQKQLDTASRFVVILARK 89
Cdd:cd02137    2 EVIKSRRSVRNFDPDHKIPKEELKEILELATLAPSSFNLQPWRFVVVRDPELKAKLAEAAYN-QPQVTTASAVILVLGDL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  90 NVTaqsdyvqhilrdikkydthtipaveqkfndfqtnfhindsertlldwaskqtyIALANMMTSAALLGIDSCPMEGFD 169
Cdd:cd02137   81 NAG-----------------------------------------------------LAAMNLMLAAKAKGYDTCPMGGFD 107

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 488409238 170 LDKVTEILQDEGvidtvHFAPSVMVAFGYRKDEPKGKVRQPATDV 214
Cdd:cd02137  108 KEKVAELLNLPD-----RYVPVLLIAIGKAADKAPRSGRLPVDEV 147
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 15-198 3.53e-29

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 107.09  E-value: 3.53e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238   15 RHATKIFDSSKqVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKPYSWGAQKQLDTASRFVVilarknvtaq 94
Cdd:pfam00881   4 RRSVRKFDPEP-VPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELLLVEPAAALLLL---------- 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238   95 sDYVQHILRDIKKYDTHTIPAVeqKFNDFQTNFHINDSERTLLDWASKQTYIALANMMTSAALLGIDSCPMEGFDLDKVT 174
Cdd:pfam00881  73 -LRRDANLKLLLQDFLRGAPVL--IVITASLSTYLRKAAERAYREALLDAGAAAQNLLLAATSLGLGSCPIGGFDAAAVR 149

                         170       180
                  ....*....|....*....|....
gi 488409238  175 EILQdegvIDTVHFaPSVMVAFGY 198
Cdd:pfam00881 150 ELLG----LPDDER-LVGLIAVGY 168
nitroreductase cd03370
uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus ...
 25-202 8.01e-25

uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus thermophilus NADH oxidase and other, uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380327 [Multi-domain]  Cd Length: 191  Bit Score: 96.23  E-value: 8.01e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  25 KQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKPYSWGaQKQLDTASRFVVILA-RKNVTAQSDYVQHilr 103
Cdd:cd03370   17 EPVPDEDLREILRLAGLAPSAWNIQPWRFVVVRDAELKEQLQAAAYG-QAQVTSAPAVIVIYSdMEDALANLEETIH--- 92

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238 104 dikkydthtiPAV-----EQKFNDFQTNF-HINDSERTllDWASKQTYIALANMMTSAALLGIDSCPMEGFDLDKVTEIL 177
Cdd:cd03370   93 ----------PGLseerrQREAAGLRGAFgKMSVEQRG--QWGLAQANIALGFLLLAAQSLGYDTSPMLGFDPEKVKALL 160

                        170       180
                 ....*....|....*....|....*
gi 488409238 178 qdeGVIDTVHFApsVMVAFGYRKDE 202
Cdd:cd03370  161 ---GLPEHVTIA--ALVALGKPAEE 180
Nitro_FMN_reductase cd02062
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 15-198 7.61e-21

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380311 [Multi-domain]  Cd Length: 139  Bit Score: 84.27  E-value: 7.61e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  15 RHATKIFDSsKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKPYSWGAQKQLDTASRFVVILARKNVTAQ 94
Cdd:cd02062    4 RRSIRKFTD-KPVPEEKLRKILEAARLAPSAGNLQPWRFIVVRDREKKEKLAKLAAPNQKFIAGAPVVIVVVADPDKSRP 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  95 sdyvqhilrdikkydthtipaveqkfndfqtnfhindsertlldWASKQTYIALANMMTSAALLGIDSCPMEGFDL--DK 172
Cdd:cd02062   83 --------------------------------------------WALEDAGAAAQNLLLAAAALGLGSCWIGGFDFreDK 118

                        170       180
                 ....*....|....*....|....*.
gi 488409238 173 VTEILQdegvIDTvHFAPSVMVAFGY 198
Cdd:cd02062  119 VRELLG----IPE-NLRPVALIAIGY 139
TdsD-like cd02138
nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase ...
 15-202 2.74e-15

nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to Burkholderia pseudomallei TdsD, may be involved in the processing of organosulfur compounds. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380315 [Multi-domain]  Cd Length: 174  Bit Score: 70.65  E-value: 2.74e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  15 RHATKIFDSsKQVSDKDMQTILESGRLSPSSLGLEPWRFVV-IESQELKDK----LKPY--SWgAQKqldtASRFVVILA 87
Cdd:cd02138    5 RWSPRAFSP-EPISEEDLLSLFEAARWAPSCFNEQPWRFVVaRRDTEAFEKlldlLAEGnqSW-AKN----APVLIVVLA 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  88 RKNvtaqsdyvqhilrdikkydthtipaveqkfndFQTNFHINdsertllDWASKQTYIALANMMTSAALLGIDSCPMEG 167
Cdd:cd02138   79 KTE--------------------------------FDHNGKPN-------RYALFDTGAAVANLALQATALGLVVHQMAG 119

                        170       180       190
                 ....*....|....*....|....*....|....*
gi 488409238 168 FDLDKVTEILQdegvIDTvHFAPSVMVAFGYRKDE 202
Cdd:cd02138  120 FDPEKAKEALG----IPD-EYEPITMIAIGYPGDP 149
nitroreductase cd02139
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 24-215 7.26e-15

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380316 [Multi-domain]  Cd Length: 165  Bit Score: 69.42  E-value: 7.26e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  24 SKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKPYSWGaQKQLDTASrfVVILArknVTAQSDYVQhilR 103
Cdd:cd02139   16 PTPVEEEKLLRILEAARLAPSAKNRQPWRFIVVKDKELKEKLAEAANG-QKFIAEAP--VVIVA---CADPSESGM---G 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238 104 DIKKYdtHTIpaveqkfndfqtnfhindsertllDWAskqtyIALANMMTSAALLGIDSCPMEGFDLDKVTEILqdeGVI 183
Cdd:cd02139   87 CGKPY--YLV------------------------DVA-----IAMEHLVLAATEEGLGTCWIGAFDEDKVKEIL---GIP 132

                        170       180       190
                 ....*....|....*....|....*....|....
gi 488409238 184 DTVHfapsV--MVAFGYRKDEPKGKVRQPATDVI 215
Cdd:cd02139  133 EEYR----VvaLTPLGYPAEEPPPRPRKPLEEIV 162
nitroreductase cd20608
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 10-198 1.81e-13

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380329 [Multi-domain]  Cd Length: 145  Bit Score: 65.05  E-value: 1.81e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  10 NALNFRHATKIFdSSKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKPYSWGAQKQLDTASRFVVILARK 89
Cdd:cd20608    2 EAIKTRRSVRRF-SDKPVEEEKLEKILEAARLAPSWANKQCWRFIVVTDKETLSELAKKESPSNGWLKDAPVIIVVCADP 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  90 NVTAQsdyvqhiLRDIKKYdthtipaveqkfndfqtnfhindsertLLDWAskqtyIALANMMTSAALLGIDSCPMEGFD 169
Cdd:cd20608   81 KDSGW-------LNGQNYY---------------------------LVDAA-----IAMQNLMLAATDLGLGTCWIGAFD 121

                        170       180
                 ....*....|....*....|....*....
gi 488409238 170 LDKVTEILqdeGVIDTVHFApsVMVAFGY 198
Cdd:cd20608  122 EKKVKEIL---GIPENIRVV--ALTPLGY 145
nitroreductase cd20609
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 15-198 2.85e-12

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380330 [Multi-domain]  Cd Length: 145  Bit Score: 62.02  E-value: 2.85e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  15 RHATKIFdSSKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLK---PYSWGAQkqldtasrfVVILarknV 91
Cdd:cd20609    9 RYSVRKF-SDKPVEKEKLDKILEAGRLAPTAVNYQPQRILVVRSEEALEKLAkatPRFFGAP---------LVIV----V 74

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  92 TAQSDYVQHilrdiKKYDTHtipaveqkfndfqtNFHINDsertlldwASkqtyIALANMMTSAALLGIDSCPMEGFDLD 171
Cdd:cd20609   75 CYDKDESWK-----RPYDGK--------------DSGDID--------AA----IVATHMMLAATELGLGTCWVGNFDPE 123

                        170       180
                 ....*....|....*....|....*...
gi 488409238 172 KVTEILQ-DEGVIdtvhfaPSVMVAFGY 198
Cdd:cd20609  124 KVREAFNlPENLE------PVAILPLGY 145
nitroreductase cd02151
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 24-92 1.13e-09

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers..


Pssm-ID: 380326 [Multi-domain]  Cd Length: 157  Bit Score: 55.23  E-value: 1.13e-09
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 488409238  24 SKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKPYSWGAQKQLDTASRFVVILARKNVT 92
Cdd:cd02151   14 DEPIEEEKLEEILEAALLAPSSRNSRPVEFIVVDDKETLKKLSECKPHGSAFLKGAPAAIVVLADTEKS 82
nitroreductase cd02150
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 23-208 2.49e-09

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380325 [Multi-domain]  Cd Length: 156  Bit Score: 54.14  E-value: 2.49e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  23 SSKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLK---PYSwgaqKQLDTASRFVVILArknvtaqsdyvq 99
Cdd:cd02150   11 TDKPVEEEDIEKLLRAAMAAPSAGNQQPWHFIVVTDREKLDKIAeahPYG----KMLKEAPLAIVVCG------------ 74

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238 100 hilrDIKKydthtipaveQKFNDFqtnfhindsertlldW---ASkqtyIALANMMTSAALLGIDSC-----PMEGfDLD 171
Cdd:cd02150   75 ----DPSK----------EKAPGY---------------WvqdCS----AATENILLAAHALGLGAVwlgvyPFEE-RVK 120

                        170       180       190
                 ....*....|....*....|....*....|....*...
gi 488409238 172 KVTEILQ-DEGVIdtvhfaPSVMVAFGYRKDEPKGKVR 208
Cdd:cd02150  121 AIREILNiPENII------PFCVIALGYPAEEKEPKDR 152
PnbA_NfnB-like cd02136
nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as ...
 11-72 1.43e-07

nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as a cofactor and catalyze reduction of a variety of nitroaromatic compounds, including nitrofurans, nitrobenzens, nitrophenol, nitrobenzoate and quinones by using either NADH or NADPH as a source of reducing equivalents in an obligatory two-election transfer mechanism. The enzyme is typically a homodimer. Mycobacterium smegmatis nitroreductase NfnB plays a role in resistance to benzothiazinone.


Pssm-ID: 380313 [Multi-domain]  Cd Length: 152  Bit Score: 49.12  E-value: 1.43e-07
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 488409238  11 ALNFRHATKIFDSsKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIeSQELKDKLKPYSWGA 72
Cdd:cd02136    1 AIKSRRSVRAFKD-KPVPKETIEKILEAARRAPSGKNTQPWRVYVV-TGKARERLKKAFFGA 60
NfsA-like cd02146
nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin ...
 25-210 3.34e-07

nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.


Pssm-ID: 380322 [Multi-domain]  Cd Length: 229  Bit Score: 49.16  E-value: 3.34e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  25 KQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKPYSwGAQKQLDTASRFVVILArknvtaqsDYVQHILrd 104
Cdd:cd02146   17 EPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKLAELA-GNQPYVAQAPVFLVFCA--------DLYRHQK-- 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238 105 ikkydthtipAVEQKFNDfqtNFHINDSERTLLdwASKQTYIALANMMTSAALLGIDSCPMEGF--DLDKVTEILQ-DEG 181
Cdd:cd02146   86 ----------IAEEAGGK---DVGLDYLESFLV--GVVDAALAAQNALVAAESLGLGIVYIGGIrnNPEEVIELLGlPEY 150

                        170       180
                 ....*....|....*....|....*....
gi 488409238 182 VIdtvhfaPSVMVAFGYRKDEPKGKVRQP 210
Cdd:cd02146  151 VF------PLFGLTVGHPDPTPEVKPRLP 173
nitroreductase cd20610
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 15-182 1.13e-05

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380331 [Multi-domain]  Cd Length: 167  Bit Score: 44.19  E-value: 1.13e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  15 RHATKIFdSSKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKlkpyswgaqkqldtasrfVVILARKnvtaq 94
Cdd:cd20610    4 RRSIRKF-KPDPVPKEDIEKILEAANWAPSGMNRQNWEFVVVKGGEKIEK------------------IGISIKK----- 59

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238  95 sdYVQHILRDIKKYDTHTIPAVEqKFNDFQTNF--------------HINDSERTLLDWASKqtyiALANMMTSAALLGI 160
Cdd:cd20610   60 --KNEEIARLLEKVFAEKPIRFR-KFRRFFTLFggapvlvvvytepyKPPEERKPDLQSVSA----AIQNLLLAAHALGL 132

                        170       180
                 ....*....|....*....|....
gi 488409238 161 DSCPMEG--FDLDKVTEILQDEGV 182
Cdd:cd20610  133 GTCWMTGplYAEDEIEEILEIPDD 156
iodotyrosine_dehalogenase cd02144
iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the ...
 21-64 2.28e-05

iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the 3, 5 positions of L-tyosine in thyroid, liver and kidney, using NADPH as electron donor. This enzyme is a homolog of the nitroreductase family. These enzymes are usually homodimers.


Pssm-ID: 380320  Cd Length: 192  Bit Score: 43.68  E-value: 2.28e-05
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 488409238  21 FDSSKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDK 64
Cdd:cd02144   13 DFSSEPVPREVIENAIRTAGTAPSGANTQPWTFVVVSDPEIKRK 56
YdjA-like cd02135
nitroreductase family protein similar to Escherichia coli YdjA; A subfamily of the ...
 15-62 5.76e-05

nitroreductase family protein similar to Escherichia coli YdjA; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to nitroreductase YdjA from Escherichia coli. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer. Members of this family are also called NADH dehydrogenase, oxygen-insensitive NAD(P)H nitrogenase or dihydropteridine reductase.


Pssm-ID: 380312 [Multi-domain]  Cd Length: 162  Bit Score: 41.82  E-value: 5.76e-05
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 488409238  15 RHATKIFDSSKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELK 62
Cdd:cd02135    7 RRSIRKFKLTGAPPEEQLEELLEAAMWAPNHGKLEPWRFIVVTGEGRE 54
PRK10765 PRK10765
oxygen-insensitive NADPH nitroreductase;
1-102 6.08e-03

oxygen-insensitive NADPH nitroreductase;


Pssm-ID: 182710  Cd Length: 240  Bit Score: 36.88  E-value: 6.08e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409238   1 MSQMNETIINALNFRHATkifdsSKQVSDKDMQTILESGRLSPSSLGLEPWRFVVIESQELKDKLKPYSwGAQKQLDTAS 80
Cdd:PRK10765   1 MTPTIELILSHRSIRHFT-----DEPISEAQREAIINAARAASSSSFLQCSSIIRITDKALREALVELT-GGQKYVAQAA 74

                         90       100
                 ....*....|....*....|....*...
gi 488409238  81 RFVVILA------RKNVTAQSDYVQHIL 102
Cdd:PRK10765  75 EFWVFCAdfnrhlQICPDAQLGLAEQLL 102
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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