NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|488409209|ref|WP_002478594|]
View 

MULTISPECIES: methylated-DNA--[protein]-cysteine S-methyltransferase [Staphylococcus]

Protein Classification

methylated-DNA--[protein]-cysteine S-methyltransferase( domain architecture ID 11417447)

methylated-DNA--[protein]-cysteine S-methyltransferase is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA; it repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
1-163 9.69e-68

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


:

Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 203.18  E-value: 9.69e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209   1 MTYAMkYDAPVGPLTILSDGESITQVLFANQACSDEMIQTGNV-----PILQQAEAWLDCYFSGEKPQSSLKLKPEGTPF 75
Cdd:COG0350    2 IRYAI-FDTPLGPLLIAATDRGLCALSFGDDREEALLARFPAAlredpPLLAEAARQLDAYFAGERKDFDLPLDLRGTPF 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209  76 QQKVWQLLSEIPYGHVVTYGElatkIAKQLGKShMSAQAVGGAVGRNPISIIVPCHRVVGKDGSLTGFGGTIDNKIKLLQ 155
Cdd:COG0350   81 QRRVWEALRKIPYGETVTYGE----LARAIGRP-KAARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLE 155

                 ....*...
gi 488409209 156 LEGVNMSA 163
Cdd:COG0350  156 LEGALAAA 163
 
Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
1-163 9.69e-68

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 203.18  E-value: 9.69e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209   1 MTYAMkYDAPVGPLTILSDGESITQVLFANQACSDEMIQTGNV-----PILQQAEAWLDCYFSGEKPQSSLKLKPEGTPF 75
Cdd:COG0350    2 IRYAI-FDTPLGPLLIAATDRGLCALSFGDDREEALLARFPAAlredpPLLAEAARQLDAYFAGERKDFDLPLDLRGTPF 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209  76 QQKVWQLLSEIPYGHVVTYGElatkIAKQLGKShMSAQAVGGAVGRNPISIIVPCHRVVGKDGSLTGFGGTIDNKIKLLQ 155
Cdd:COG0350   81 QRRVWEALRKIPYGETVTYGE----LARAIGRP-KAARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLE 155

                 ....*...
gi 488409209 156 LEGVNMSA 163
Cdd:COG0350  156 LEGALAAA 163
PRK00901 PRK00901
methylated-DNA--protein-cysteine methyltransferase; Provisional
1-160 1.69e-48

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 234860 [Multi-domain]  Cd Length: 155  Bit Score: 154.44  E-value: 1.69e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209   1 MTYAMKYDAPVGPLTILSDGESITQVLFANQACSDEMIqTGNVPILQQAEAWLDCYFSGEKPQSSLKLKPEGTPFQQKVW 80
Cdd:PRK00901   1 MNNIYFYETPIGKIGIAENGTAITHLCFGEDKIPKDVT-ILETDLLKEANKQLEEYFEGKRKKFDLPLAPQGTEFQKKVW 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209  81 QLLSEIPYGHVVTYGElatkIAKQLGKSHmSAQAVGGAVGRNPISIIVPCHRVVGKDGSLTGFGGTIDNKIKLLQLEGVN 160
Cdd:PRK00901  80 KALQEIPYGETRSYKE----IAVNIGNPK-ACRAVGLANNKNPIPIFIPCHRVIGANGKLVGYAGGLDIKEKLLKLEKEN 154
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
74-159 5.75e-46

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 145.20  E-value: 5.75e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209   74 PFQQKVWQLLSEIPYGHVVTYGElatkIAKQLGKShMSAQAVGGAVGRNPISIIVPCHRVVGKDGSLTGFGGTIDNKIKL 153
Cdd:pfam01035   1 PFQRRVWEALRQIPYGKTTTYGE----IAKLLGRP-KAARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRAL 75

                  ....*.
gi 488409209  154 LQLEGV 159
Cdd:pfam01035  76 LELEGV 81
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
75-158 6.53e-39

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 127.21  E-value: 6.53e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209  75 FQQKVWQLLSEIPYGHVVTYGElatkIAKQLGKShMSAQAVGGAVGRNPISIIVPCHRVVGKDGSLTGFGGTIDNKIKLL 154
Cdd:cd06445    1 FQRRVWEALRQIPYGEVTTYGQ----IAKLAGTP-KAARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELL 75

                 ....
gi 488409209 155 QLEG 158
Cdd:cd06445   76 ELEG 79
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
73-157 2.49e-38

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 125.89  E-value: 2.49e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209   73 TPFQQKVWQLLSEIPYGHVVTYGELAtkiaKQLGKSHmSAQAVGGAVGRNPISIIVPCHRVVGKDGSLTGFGGTIDNKIK 152
Cdd:TIGR00589   1 TPFQQKVWKALRTIPYGETKSYGQLA----KAIGNPK-AARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKEF 75

                  ....*
gi 488409209  153 LLQLE 157
Cdd:TIGR00589  76 LLEHE 80
 
Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
1-163 9.69e-68

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 203.18  E-value: 9.69e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209   1 MTYAMkYDAPVGPLTILSDGESITQVLFANQACSDEMIQTGNV-----PILQQAEAWLDCYFSGEKPQSSLKLKPEGTPF 75
Cdd:COG0350    2 IRYAI-FDTPLGPLLIAATDRGLCALSFGDDREEALLARFPAAlredpPLLAEAARQLDAYFAGERKDFDLPLDLRGTPF 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209  76 QQKVWQLLSEIPYGHVVTYGElatkIAKQLGKShMSAQAVGGAVGRNPISIIVPCHRVVGKDGSLTGFGGTIDNKIKLLQ 155
Cdd:COG0350   81 QRRVWEALRKIPYGETVTYGE----LARAIGRP-KAARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLE 155

                 ....*...
gi 488409209 156 LEGVNMSA 163
Cdd:COG0350  156 LEGALAAA 163
PRK00901 PRK00901
methylated-DNA--protein-cysteine methyltransferase; Provisional
1-160 1.69e-48

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 234860 [Multi-domain]  Cd Length: 155  Bit Score: 154.44  E-value: 1.69e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209   1 MTYAMKYDAPVGPLTILSDGESITQVLFANQACSDEMIqTGNVPILQQAEAWLDCYFSGEKPQSSLKLKPEGTPFQQKVW 80
Cdd:PRK00901   1 MNNIYFYETPIGKIGIAENGTAITHLCFGEDKIPKDVT-ILETDLLKEANKQLEEYFEGKRKKFDLPLAPQGTEFQKKVW 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209  81 QLLSEIPYGHVVTYGElatkIAKQLGKSHmSAQAVGGAVGRNPISIIVPCHRVVGKDGSLTGFGGTIDNKIKLLQLEGVN 160
Cdd:PRK00901  80 KALQEIPYGETRSYKE----IAVNIGNPK-ACRAVGLANNKNPIPIFIPCHRVIGANGKLVGYAGGLDIKEKLLKLEKEN 154
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
74-159 5.75e-46

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 145.20  E-value: 5.75e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209   74 PFQQKVWQLLSEIPYGHVVTYGElatkIAKQLGKShMSAQAVGGAVGRNPISIIVPCHRVVGKDGSLTGFGGTIDNKIKL 153
Cdd:pfam01035   1 PFQRRVWEALRQIPYGKTTTYGE----IAKLLGRP-KAARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRAL 75

                  ....*.
gi 488409209  154 LQLEGV 159
Cdd:pfam01035  76 LELEGV 81
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
75-158 6.53e-39

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 127.21  E-value: 6.53e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209  75 FQQKVWQLLSEIPYGHVVTYGElatkIAKQLGKShMSAQAVGGAVGRNPISIIVPCHRVVGKDGSLTGFGGTIDNKIKLL 154
Cdd:cd06445    1 FQRRVWEALRQIPYGEVTTYGQ----IAKLAGTP-KAARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELL 75

                 ....
gi 488409209 155 QLEG 158
Cdd:cd06445   76 ELEG 79
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
73-157 2.49e-38

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 125.89  E-value: 2.49e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209   73 TPFQQKVWQLLSEIPYGHVVTYGELAtkiaKQLGKSHmSAQAVGGAVGRNPISIIVPCHRVVGKDGSLTGFGGTIDNKIK 152
Cdd:TIGR00589   1 TPFQQKVWKALRTIPYGETKSYGQLA----KAIGNPK-AARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKEF 75

                  ....*
gi 488409209  153 LLQLE 157
Cdd:TIGR00589  76 LLEHE 80
PRK10286 PRK10286
methylated-DNA--[protein]-cysteine S-methyltransferase;
6-158 1.72e-31

methylated-DNA--[protein]-cysteine S-methyltransferase;


Pssm-ID: 182355 [Multi-domain]  Cd Length: 171  Bit Score: 111.50  E-value: 1.72e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209   6 KYDAPVGPLTILSDGESITQVLFANQAcSDEMIQTGNVPILQQAEAWLDC------------YFSGE-KPQSSLKLKPEG 72
Cdd:PRK10286   8 KIATPLGPLWVICDEQFRLRAVEWEEY-SERMVQLLDIHYRKEGYERISAtnpgglsdklrdYFAGNlSIIDTLPTATGG 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209  73 TPFQQKVWQLLSEIPYGHVVTYGELAtkiaKQLGKSHmSAQAVGGAVGRNPISIIVPCHRVVGKDGSLTGFGGTIDNKIK 152
Cdd:PRK10286  87 TPFQREVWQTLRTIPCGQVMHYGQLA----EQLGRPG-AARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKEW 161

                 ....*.
gi 488409209 153 LLQLEG 158
Cdd:PRK10286 162 LLRHEG 167
PRK15435 PRK15435
bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;
48-157 8.53e-22

bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;


Pssm-ID: 185333 [Multi-domain]  Cd Length: 353  Bit Score: 89.85  E-value: 8.53e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209  48 QAEAWLDcyfSGEKPQSsLKLKPEGTPFQQKVWQLLSEIPYGHVVTYGELATKIAKQlgkshMSAQAVGGAVGRNPISII 127
Cdd:PRK15435 248 EVIASLN---QRDTPLT-LPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKP-----KAVRAVASACAANKLAIV 318
                         90       100       110
                 ....*....|....*....|....*....|
gi 488409209 128 VPCHRVVGKDGSLTGFGGTIDNKIKLLQLE 157
Cdd:PRK15435 319 IPCHRVVRGDGALSGYRWGVSRKAQLLRRE 348
Atl1 COG3695
Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like ...
73-166 1.62e-21

Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like DNA-binding domain [Transcription];


Pssm-ID: 442910  Cd Length: 104  Bit Score: 83.70  E-value: 1.62e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209  73 TPFQQKVWQLLSEIPYGHVVTYGElatkIAKQLGkSHMSAQAVGGAVGRNPISIIVPCHRVVGKDGSLT-GFGGTIDNKI 151
Cdd:COG3695    4 EEFYERVYEVVAQIPPGRVATYGD----IAALAG-LPRGARQVGRALRALPEGSDLPWHRVVNADGRLSpGHAGGAEEQR 78
                         90
                 ....*....|....*
gi 488409209 152 KLLQLEGVNMSALYR 166
Cdd:COG3695   79 ELLEAEGVPVVDDGR 93
PRK03887 PRK03887
methylated-DNA--protein-cysteine methyltransferase; Provisional
73-159 2.08e-18

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 167628 [Multi-domain]  Cd Length: 175  Bit Score: 77.85  E-value: 2.08e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209  73 TPFQQKVWQLLSE-IPYGHVVTYGELAtkiaKQLGKShmsAQAVGGAVGRNPISIIVPCHRVVGKDGsLTGFGGTIDNKI 151
Cdd:PRK03887  91 TPFERKVYEWLTKnVKRGEVITYGELA----KALNTS---PRAVGGAMKRNPYPIIVPCHRVVGRKN-PGLYTPKPEYKK 162

                 ....*...
gi 488409209 152 KLLQLEGV 159
Cdd:PRK03887 163 FLLEVEGV 170
AdaA COG2169
Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), ...
38-157 5.29e-17

Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), contains Zn-binding and two AraC-type DNA-binding domains [Replication, recombination and repair];


Pssm-ID: 441772 [Multi-domain]  Cd Length: 358  Bit Score: 76.63  E-value: 5.29e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488409209  38 IQTGNVPILQQAEAWLDcYFSGEKPQSSLKLKPEGTPFQQKVWQLLSEIPYGHVVTYGELATKIAKQLgkshmSAQAVGG 117
Cdd:COG2169  236 LIGGDAAFEQLVAEVVG-FVEGPLLGLDLPLDLRGTAFQQRVWQALRAIPAGETASYAEIAARIGAPK-----AVRAVAA 309
                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 488409209 118 AVGRNPISIIVPCHRVVGKDGSLTGFGGTIDNKIKLLQLE 157
Cdd:COG2169  310 ACAANQLAVAIPCHRVVRADGALSGYRWGVERKRALLERE 349
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH