GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both CoA and acetyl-CoA. They are characterized by highly conserved glycine, a cysteine residue in the acetyl-CoA binding site near the acetyl group, their small size compared with other GNATs and a lack of of an obvious substrate-binding site. It is proposed that they transfer an acetyl group from acetyl-CoA to one or more unidentified aliphatic amines via an acetyl (cysteine) enzyme intermediate. The substrate might be another macromolecule.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487749586  11 KFYVGNDENNPKAVITFKeVDNNEIDIDHTGVSDELGGQGLGKKLVNRVVEHARENNLKIIASCPFAKNVLEKDDHAQDV 90
Cdd:pfam14542  1 RFEIRVDGGAEVAFLTYR-RGDGVLIITHTEVPPALRGQGIASKLVKAALDDAREEGLKIVPLCSYVAAYLEKHPEYADL 79

GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both CoA and acetyl-CoA. They are characterized by highly conserved glycine, a cysteine residue in the acetyl-CoA binding site near the acetyl group, their small size compared with other GNATs and a lack of of an obvious substrate-binding site. It is proposed that they transfer an acetyl group from acetyl-CoA to one or more unidentified aliphatic amines via an acetyl (cysteine) enzyme intermediate. The substrate might be another macromolecule.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487749586  11 KFYVGNDENNPKAVITFKeVDNNEIDIDHTGVSDELGGQGLGKKLVNRVVEHARENNLKIIASCPFAKNVLEKDDHAQDV 90
Cdd:pfam14542  1 RFEIRVDGGAEVAFLTYR-RGDGVLIITHTEVPPALRGQGIASKLVKAALDDAREEGLKIVPLCSYVAAYLEKHPEYADL 79

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.

                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 487749586 12 FYVGNDENNPKAVITFKEVD--NNEIDIDHTGVSDELGGQGLGKKLVNRVVEHARENNLKII 71
Cdd:cd04301   1 FLVAEDDGEIVGFASLSPDGsgGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRL 62

GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both CoA and acetyl-CoA. They are characterized by highly conserved glycine, a cysteine residue in the acetyl-CoA binding site near the acetyl group, their small size compared with other GNATs and a lack of of an obvious substrate-binding site. It is proposed that they transfer an acetyl group from acetyl-CoA to one or more unidentified aliphatic amines via an acetyl (cysteine) enzyme intermediate. The substrate might be another macromolecule.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487749586  11 KFYVGNDENNPKAVITFKeVDNNEIDIDHTGVSDELGGQGLGKKLVNRVVEHARENNLKIIASCPFAKNVLEKDDHAQDV 90
Cdd:pfam14542  1 RFEIRVDGGAEVAFLTYR-RGDGVLIITHTEVPPALRGQGIASKLVKAALDDAREEGLKIVPLCSYVAAYLEKHPEYADL 79

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487749586   1 MAAEIKQ---GDHK--FYVGNDENNPKAVITFKEVDNNEIDIDHTGVSDELGGQGLGKKLVNRVVEHARENNLKIIAScp 75
Cdd:COG0454   20 LDAELKAmegSLAGaeFIAVDDKGEPIGFAGLRRLDDKVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALEL-- 97

                         90
                 ....*....|....*..
gi 487749586  76 fakNVLEKDDHAQDVYL 92
Cdd:COG0454   98 ---DTLDGNPAAIRFYE 111

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487749586    1 MAAEIKQGDHKFYVGNDENNPKAVITFKEVDN--NEIDIDHTGVSDELGGQGLGKKLVNRVVEHARENNLKIIASCPFAK 78
Cdd:pfam00583  24 LEDWDEDASEGFFVAEEDGELVGFASLSIIDDepPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAAD 103

                          90
                  ....*....|...
gi 487749586   79 NvlekdDHAQDVY 91
Cdd:pfam00583 104 N-----LAAIALY 111

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 487749586   5 IKQGDHKFYVGNDENNPKAVITFKEVDNNEIDIDHTGVSDELGGQGLGKKLVNRVVEHARENNLK 69
Cdd:COG1246   23 LEEEIGEFWVAEEDGEIVGCAALHPLDEDLAELRSLAVHPDYRGRGIGRRLLEALLAEARELGLK 87

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 487749586  34 EIDIdhtGVSDELGGQGLGKKLVNRVVEHARENNLKIIASCPFAKN 79
Cdd:COG1247   83 EESI---YVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADN 125

Predicted N-acyltransferase, GNAT family [General function prediction only];

                         10        20
                 ....*....|....*....|....
gi 487749586  48 GQGLGKKLVNRVVEHARENNLKII 71
Cdd:COG2153   72 GQGLGRALMEAAIEEARERGARRI 95