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Conserved domains on  [gi|485708907|ref|WP_001341474|]
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IS66 family insertion sequence element accessory protein TnpB [Escherichia coli]

Protein Classification

transposase( domain architecture ID 10529723)

transposase binds to the end of a transposon and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism

Gene Ontology:  GO:0004803|GO:0003677|GO:0006313
PubMed:  30454069|11418571|20215432

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TnpB_IS66 pfam05717
IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The ...
 1-88 8.01e-43

IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The function of these proteins is uncertain, but they are probably essential for transposition.


:

Pssm-ID: 461723  Cd Length: 96  Bit Score: 134.52  E-value: 8.01e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 485708907   1 MRKSFNGLGEQVQHVLDENPFSGHLFIFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPA-VRDGKVSITRSQLAMLL 79
Cdd:pfam05717  7 MRKGIDGLAALVQEALGLDPFSGHLFVFRNRRRDRIKILYWDGDGFWLYYKRLERGRFVWPRrAGDGVVELTAAQLSWLL 86


                 ....*....
gi 485708907  80 DKLDWRQPK 88
Cdd:pfam05717 87 EGIDWRRPR 95
 
Name Accession Description Interval E-value
TnpB_IS66 pfam05717
IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The ...
 1-88 8.01e-43

IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The function of these proteins is uncertain, but they are probably essential for transposition.


Pssm-ID: 461723  Cd Length: 96  Bit Score: 134.52  E-value: 8.01e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 485708907   1 MRKSFNGLGEQVQHVLDENPFSGHLFIFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPA-VRDGKVSITRSQLAMLL 79
Cdd:pfam05717  7 MRKGIDGLAALVQEALGLDPFSGHLFVFRNRRRDRIKILYWDGDGFWLYYKRLERGRFVWPRrAGDGVVELTAAQLSWLL 86


                 ....*....
gi 485708907  80 DKLDWRQPK 88
Cdd:pfam05717 87 EGIDWRRPR 95
IS66_TnpB NF033819
IS66 family insertion sequence element accessory protein TnpB; The IS66 family insertion ...
 1-80 3.37e-42

IS66 family insertion sequence element accessory protein TnpB; The IS66 family insertion sequence element encodes a DDE transposase TnpC, and two accessory proteins, TnpA and TnpB. It has been assumed that the TnpA, TnpB, and TnpC proteins are produced independently in appropriate amounts and form a complex, which acts as a transposase to promote the transposition of an IS66 family element.


Pssm-ID: 411392  Cd Length: 90  Bit Score: 132.99  E-value: 3.37e-42
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 485708907  1 MRKSFNGLGEQVQHVLDENPFSGHLFIFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLD 80
Cdd:NF033819 10 MRKGIDGLAALVQEVLGLDPFSGHLFVFRNRRRDRLKILYWDGSGFCLYYKRLEKGRFRWPRAADGVVALTPAQLAWLLE 89
COG3436 COG3436
Transposase [Mobilome: prophages, transposons];
8-94 3.10e-06

Transposase [Mobilome: prophages, transposons];


Pssm-ID: 442662 [Multi-domain]  Cd Length: 416  Bit Score: 43.48  E-value: 3.10e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 485708907   8 LGEQVQHVLDENPfsGHLFIFRGRRgDTIKILWADADGLC---LFTKRLEEGQF---------------IWPAVR----- 64
Cdd:COG3436   95 IGEEVSEELDLDP--GALFVFRNRR-DKYKCLYCDGTGLVyapAPAKRLEKGLAgpgllahilvskfvdHLPLYRqeeif 171

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 485708907  65 -DGKVSITRSQLA-----------MLLDKLDWRQPKTSRLNA 94
Cdd:COG3436  172 aRLGVELSRSTLLnwvgraaellePLLEGLDERLLAAPVLHA 213
 
Name Accession Description Interval E-value
TnpB_IS66 pfam05717
IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The ...
 1-88 8.01e-43

IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The function of these proteins is uncertain, but they are probably essential for transposition.


Pssm-ID: 461723  Cd Length: 96  Bit Score: 134.52  E-value: 8.01e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 485708907   1 MRKSFNGLGEQVQHVLDENPFSGHLFIFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPA-VRDGKVSITRSQLAMLL 79
Cdd:pfam05717  7 MRKGIDGLAALVQEALGLDPFSGHLFVFRNRRRDRIKILYWDGDGFWLYYKRLERGRFVWPRrAGDGVVELTAAQLSWLL 86


                 ....*....
gi 485708907  80 DKLDWRQPK 88
Cdd:pfam05717 87 EGIDWRRPR 95
IS66_TnpB NF033819
IS66 family insertion sequence element accessory protein TnpB; The IS66 family insertion ...
 1-80 3.37e-42

IS66 family insertion sequence element accessory protein TnpB; The IS66 family insertion sequence element encodes a DDE transposase TnpC, and two accessory proteins, TnpA and TnpB. It has been assumed that the TnpA, TnpB, and TnpC proteins are produced independently in appropriate amounts and form a complex, which acts as a transposase to promote the transposition of an IS66 family element.


Pssm-ID: 411392  Cd Length: 90  Bit Score: 132.99  E-value: 3.37e-42
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 485708907  1 MRKSFNGLGEQVQHVLDENPFSGHLFIFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLD 80
Cdd:NF033819 10 MRKGIDGLAALVQEVLGLDPFSGHLFVFRNRRRDRLKILYWDGSGFCLYYKRLEKGRFRWPRAADGVVALTPAQLAWLLE 89
COG3436 COG3436
Transposase [Mobilome: prophages, transposons];
8-94 3.10e-06

Transposase [Mobilome: prophages, transposons];


Pssm-ID: 442662 [Multi-domain]  Cd Length: 416  Bit Score: 43.48  E-value: 3.10e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 485708907   8 LGEQVQHVLDENPfsGHLFIFRGRRgDTIKILWADADGLC---LFTKRLEEGQF---------------IWPAVR----- 64
Cdd:COG3436   95 IGEEVSEELDLDP--GALFVFRNRR-DKYKCLYCDGTGLVyapAPAKRLEKGLAgpgllahilvskfvdHLPLYRqeeif 171

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 485708907  65 -DGKVSITRSQLA-----------MLLDKLDWRQPKTSRLNA 94
Cdd:COG3436  172 aRLGVELSRSTLLnwvgraaellePLLEGLDERLLAAPVLHA 213
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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