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Conserved domains on  [gi|447185096|ref|WP_001262352|]
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MULTISPECIES: hypothetical protein [Enterobacteriaceae]

Protein Classification

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
DnaT super family cl46275
DnaT DNA-binding domain; This domain is found in E.coli primosomal protein 1 (Pp1). PP1 domain ...
337-356 1.60e-03

DnaT DNA-binding domain; This domain is found in E.coli primosomal protein 1 (Pp1). PP1 domain (residues 84-153) in Swiss:P0A8J2 can bind to different types of ssDNA, which is fundamental for its physiological substrate bindings. Functional analysis indicate that both N- and C- terminals are essential to having the cooperative effect in binding ssDNA. The ssDNA bound complex displays a spiral filament assembly that is adopted by many proteins that are involved in DNA replication, such as DnaA, RecA and PriB. This domain is similar to pfam08585 except that it contains an extra loop at the N-terminus (84-99). Structural analysis indicate that this extra loop might be essential for the stabilization of the three-helix bundle.


The actual alignment was detected with superfamily member PRK02854:

Pssm-ID: 480615  Cd Length: 179  Bit Score: 39.02  E-value: 1.60e-03
                         10        20
                 ....*....|....*....|
gi 447185096 337 QRCVNHISEPDNEIPPGFRG 356
Cdd:PRK02854 160 KRDINTVSEPDSQIPPGFRG 179
 
Name Accession Description Interval E-value
PRK02854 PRK02854
primosomal protein DnaT;
337-356 1.60e-03

primosomal protein DnaT;


Pssm-ID: 179484  Cd Length: 179  Bit Score: 39.02  E-value: 1.60e-03
                         10        20
                 ....*....|....*....|
gi 447185096 337 QRCVNHISEPDNEIPPGFRG 356
Cdd:PRK02854 160 KRDINTVSEPDSQIPPGFRG 179
 
Name Accession Description Interval E-value
PRK02854 PRK02854
primosomal protein DnaT;
337-356 1.60e-03

primosomal protein DnaT;


Pssm-ID: 179484  Cd Length: 179  Bit Score: 39.02  E-value: 1.60e-03
                         10        20
                 ....*....|....*....|
gi 447185096 337 QRCVNHISEPDNEIPPGFRG 356
Cdd:PRK02854 160 KRDINTVSEPDSQIPPGFRG 179
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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