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Conserved domains on  [gi|446731239|ref|WP_000808495|]
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MULTISPECIES: molybdenum cofactor biosynthesis protein MoaE [Staphylococcus]

Protein Classification

molybdenum cofactor biosynthesis protein MoaE( domain architecture ID 10001056)

molybdenum cofactor biosynthesis protein MoaE serves as the catalytic subunit of the molybdopterin synthase complex, which catalyzes the conversion of precursor Z into molybdopterin

CATH:  3.90.1170.40
EC:  2.8.1.12
Gene Ontology:  GO:0006777|GO:0030366
PubMed:  35744859
SCOP:  4001136

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MoaE COG0314
Molybdopterin synthase catalytic subunit MoaE [Coenzyme transport and metabolism]; ...
 1-133 7.09e-59

Molybdopterin synthase catalytic subunit MoaE [Coenzyme transport and metabolism]; Molybdopterin synthase catalytic subunit MoaE is part of the Pathway/BioSystem: Molybdopterin biosynthesis


:

Pssm-ID: 440083 [Multi-domain]  Cd Length: 143  Bit Score: 179.22  E-value: 7.09e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446731239   1 MKQFEIVIEPIQTEQ-YREFTI-NEYQGAVVVFTGHVREWTKGVKTEYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSI 78
Cdd:COG0314    1 MMRVRVTEEPFDLAAeLAALRAsDPEAGAVVTFVGTVRDHNDGRRVTALEYEAYPPMAEKELAEIAEEAAERWGLLDVAV 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 446731239  79 VHRIGPLQISDIAVLIAVSSPHRKDAYRANEYAIERIKEIVPIWKKEIWEDGSKW 133
Cdd:COG0314   81 IHRVGRLEPGEPIVLVAVSSAHRKEAFEACRFLIDRLKTRAPIWKKEIFEDGEEW 135
 
Name Accession Description Interval E-value
MoaE COG0314
Molybdopterin synthase catalytic subunit MoaE [Coenzyme transport and metabolism]; ...
 1-133 7.09e-59

Molybdopterin synthase catalytic subunit MoaE [Coenzyme transport and metabolism]; Molybdopterin synthase catalytic subunit MoaE is part of the Pathway/BioSystem: Molybdopterin biosynthesis


Pssm-ID: 440083 [Multi-domain]  Cd Length: 143  Bit Score: 179.22  E-value: 7.09e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446731239   1 MKQFEIVIEPIQTEQ-YREFTI-NEYQGAVVVFTGHVREWTKGVKTEYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSI 78
Cdd:COG0314    1 MMRVRVTEEPFDLAAeLAALRAsDPEAGAVVTFVGTVRDHNDGRRVTALEYEAYPPMAEKELAEIAEEAAERWGLLDVAV 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 446731239  79 VHRIGPLQISDIAVLIAVSSPHRKDAYRANEYAIERIKEIVPIWKKEIWEDGSKW 133
Cdd:COG0314   81 IHRVGRLEPGEPIVLVAVSSAHRKEAFEACRFLIDRLKTRAPIWKKEIFEDGEEW 135
MoaE cd00756
MoaE family. Members of this family are involved in biosynthesis of the molybdenum cofactor ...
 26-133 7.17e-53

MoaE family. Members of this family are involved in biosynthesis of the molybdenum cofactor (Moco), an essential cofactor for a diverse group of redox enzymes. Moco biosynthesis is an evolutionarily conserved pathway present in eubacteria, archaea and eukaryotes. Moco contains a tricyclic pyranopterin, termed molybdopterin (MPT), which carries the cis-dithiolene group responsible for molybdenum ligation. This dithiolene group is generated by MPT synthase in the second major step in Moco biosynthesis. MPT synthase is a heterotetramer consisting of two large (MoaE) and two small (MoaD) subunits.


Pssm-ID: 238385 [Multi-domain]  Cd Length: 124  Bit Score: 163.07  E-value: 7.17e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446731239  26 GAVVVFTGHVREWTKGVKTEYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRKDAY 105
Cdd:cd00756   16 GAVVTFVGTVRDHDEGKGVEALEYEAYPPMAEKELEEIAEEARERWGLLRVAIIHRVGRLPPGEAIVLVAVSSPHRKEAF 95

                         90       100
                 ....*....|....*....|....*...
gi 446731239 106 RANEYAIERIKEIVPIWKKEIWEDGSKW 133
Cdd:cd00756   96 EACEFLIDRLKHRAPIWKKEIFEGGEEW 123
MoaE pfam02391
MoaE protein; This family contains the MoaE protein that is involved in biosynthesis of ...
 6-117 5.47e-49

MoaE protein; This family contains the MoaE protein that is involved in biosynthesis of molybdopterin. Molybdopterin, the universal component of the pterin molybdenum cofactors, contains a dithiolene group serving to bind Mo. Addition of the dithiolene sulfurs to a molybdopterin precursor requires the activity of the converting factor. Converting factor contains the MoaE and MoaD proteins.


Pssm-ID: 460546  Cd Length: 113  Bit Score: 152.99  E-value: 5.47e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446731239    6 IVIEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKTEYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPL 85
Cdd:pfam02391   1 ITEEPLDVAEIAALVSDPEAGAVVTFVGTVRDHFDGKKVTALEYEAYPPMAEKELAEIAEEARERWPLLDVAIVHRVGRL 80

                          90       100       110
                  ....*....|....*....|....*....|..
gi 446731239   86 QISDIAVLIAVSSPHRKDAYRANEYAIERIKE 117
Cdd:pfam02391  81 PVGEAIVLVAVSSPHRAEAFEACEYLIDELKA 112
PLN02390 PLN02390
molybdopterin synthase catalytic subunit
 26-131 2.45e-34

molybdopterin synthase catalytic subunit


Pssm-ID: 178014  Cd Length: 111  Bit Score: 115.94  E-value: 2.45e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446731239  26 GAVVVFTGHVREWTKGVKTEYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRKDAY 105
Cdd:PLN02390   5 GAIATFSGTTRDTFEGKTVLELRYEAYVPMALRELRKICDEARSRWSLHKIAVAHRLGPVPVGETSVFVAVSSVHRADAL 84

                         90       100
                 ....*....|....*....|....*.
gi 446731239 106 RANEYAIERIKEIVPIWKKEIWEDGS 131
Cdd:PLN02390  85 DACKFLIDELKASVPIWKKEVYDDGE 110
 
Name Accession Description Interval E-value
MoaE COG0314
Molybdopterin synthase catalytic subunit MoaE [Coenzyme transport and metabolism]; ...
 1-133 7.09e-59

Molybdopterin synthase catalytic subunit MoaE [Coenzyme transport and metabolism]; Molybdopterin synthase catalytic subunit MoaE is part of the Pathway/BioSystem: Molybdopterin biosynthesis


Pssm-ID: 440083 [Multi-domain]  Cd Length: 143  Bit Score: 179.22  E-value: 7.09e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446731239   1 MKQFEIVIEPIQTEQ-YREFTI-NEYQGAVVVFTGHVREWTKGVKTEYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSI 78
Cdd:COG0314    1 MMRVRVTEEPFDLAAeLAALRAsDPEAGAVVTFVGTVRDHNDGRRVTALEYEAYPPMAEKELAEIAEEAAERWGLLDVAV 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 446731239  79 VHRIGPLQISDIAVLIAVSSPHRKDAYRANEYAIERIKEIVPIWKKEIWEDGSKW 133
Cdd:COG0314   81 IHRVGRLEPGEPIVLVAVSSAHRKEAFEACRFLIDRLKTRAPIWKKEIFEDGEEW 135
MoaE cd00756
MoaE family. Members of this family are involved in biosynthesis of the molybdenum cofactor ...
 26-133 7.17e-53

MoaE family. Members of this family are involved in biosynthesis of the molybdenum cofactor (Moco), an essential cofactor for a diverse group of redox enzymes. Moco biosynthesis is an evolutionarily conserved pathway present in eubacteria, archaea and eukaryotes. Moco contains a tricyclic pyranopterin, termed molybdopterin (MPT), which carries the cis-dithiolene group responsible for molybdenum ligation. This dithiolene group is generated by MPT synthase in the second major step in Moco biosynthesis. MPT synthase is a heterotetramer consisting of two large (MoaE) and two small (MoaD) subunits.


Pssm-ID: 238385 [Multi-domain]  Cd Length: 124  Bit Score: 163.07  E-value: 7.17e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446731239  26 GAVVVFTGHVREWTKGVKTEYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRKDAY 105
Cdd:cd00756   16 GAVVTFVGTVRDHDEGKGVEALEYEAYPPMAEKELEEIAEEARERWGLLRVAIIHRVGRLPPGEAIVLVAVSSPHRKEAF 95

                         90       100
                 ....*....|....*....|....*...
gi 446731239 106 RANEYAIERIKEIVPIWKKEIWEDGSKW 133
Cdd:cd00756   96 EACEFLIDRLKHRAPIWKKEIFEGGEEW 123
MoaE pfam02391
MoaE protein; This family contains the MoaE protein that is involved in biosynthesis of ...
 6-117 5.47e-49

MoaE protein; This family contains the MoaE protein that is involved in biosynthesis of molybdopterin. Molybdopterin, the universal component of the pterin molybdenum cofactors, contains a dithiolene group serving to bind Mo. Addition of the dithiolene sulfurs to a molybdopterin precursor requires the activity of the converting factor. Converting factor contains the MoaE and MoaD proteins.


Pssm-ID: 460546  Cd Length: 113  Bit Score: 152.99  E-value: 5.47e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446731239    6 IVIEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKTEYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPL 85
Cdd:pfam02391   1 ITEEPLDVAEIAALVSDPEAGAVVTFVGTVRDHFDGKKVTALEYEAYPPMAEKELAEIAEEARERWPLLDVAIVHRVGRL 80

                          90       100       110
                  ....*....|....*....|....*....|..
gi 446731239   86 QISDIAVLIAVSSPHRKDAYRANEYAIERIKE 117
Cdd:pfam02391  81 PVGEAIVLVAVSSPHRAEAFEACEYLIDELKA 112
PLN02390 PLN02390
molybdopterin synthase catalytic subunit
 26-131 2.45e-34

molybdopterin synthase catalytic subunit


Pssm-ID: 178014  Cd Length: 111  Bit Score: 115.94  E-value: 2.45e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446731239  26 GAVVVFTGHVREWTKGVKTEYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRKDAY 105
Cdd:PLN02390   5 GAIATFSGTTRDTFEGKTVLELRYEAYVPMALRELRKICDEARSRWSLHKIAVAHRLGPVPVGETSVFVAVSSVHRADAL 84

                         90       100
                 ....*....|....*....|....*.
gi 446731239 106 RANEYAIERIKEIVPIWKKEIWEDGS 131
Cdd:PLN02390  85 DACKFLIDELKASVPIWKKEVYDDGE 110
moaE PRK10678
molybdopterin synthase catalytic subunit MoaE;
14-146 2.62e-26

molybdopterin synthase catalytic subunit MoaE;


Pssm-ID: 182642  Cd Length: 150  Bit Score: 96.74  E-value: 2.62e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446731239  14 EQYREFTINEYQGAVVVFTGHVREWTKGVKTEYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVL 93
Cdd:PRK10678  17 EEYPWLAERDEDGAVVTFTGKVRNHNLGDSVKALTLEHYPGMTEKALAEIVDEARSRWPLGRVTVIHRVGELWPGDEIVF 96

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 446731239  94 IAVSSPHRKDAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKR 146
Cdd:PRK10678  97 VGVTSAHRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARDSDQQAAKR 149
PRK14493 PRK14493
putative bifunctional molybdopterin-guanine dinucleotide biosynthesis protein MobB/MoaE; ...
 23-133 3.76e-24

putative bifunctional molybdopterin-guanine dinucleotide biosynthesis protein MobB/MoaE; Provisional


Pssm-ID: 237730 [Multi-domain]  Cd Length: 274  Bit Score: 93.91  E-value: 3.76e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446731239  23 EYQGAVVVFTGHVR--EWTKGVKTEYLEYEAYIPMAEKKLAQIGDEINEKwPGTITSIV-HRIGPLQISDIAVLIAVSSP 99
Cdd:PRK14493 157 DKAGAIATFTGRVRakEDADDEPTEYLEFEKYDGVADERMAAIREELKQR-DGVFEVLLhHRTGVIEAGEDIVFVVVLAG 235

                         90       100       110
                 ....*....|....*....|....*....|....
gi 446731239 100 HRKDAYRANEYAIERIKEIVPIWKKEIWEDGSKW 133
Cdd:PRK14493 236 HRQEAFRAVSDGIDRLKDEVPIFKKEVTVDEEFW 269
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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