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Conserved domains on  [gi|446546324|ref|WP_000623670|]
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MULTISPECIES: PTS sugar transporter subunit IIA [Enterobacteriaceae]

Protein Classification

PTS sugar transporter subunit IIA( domain architecture ID 76)

PTS sugar transporter subunit IIA is part of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system that catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane

CATH:  3.40.930.10
Gene Ontology:  GO:1902495|GO:0009401|GO:0016310|GO:0016301|GO:0008643
PubMed:  11532441|10393270
SCOP:  4001876

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PTS_IIA_fru super family cl00163
PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: ...
 1-146 7.14e-41

PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. This family is one of four structurally and functionally distinct group IIA PTS system cytoplasmic enzymes, necessary for the uptake of carbohydrates across the cytoplasmic membrane and their phosphorylation.


The actual alignment was detected with superfamily member PRK09665:

Pssm-ID: 469635 [Multi-domain]  Cd Length: 150  Bit Score: 133.77  E-value: 7.14e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446546324   1 MISISVADGAGLQNYDAVLEHIFNTHRTTNTVKESWLNAMRKREAEYPTGIELEGYSIAIPHCDSEHANQPAIYIIRLPD 80
Cdd:PRK09665   1 MSNLFVRTGISFVDRSEALTHIGNEMLAKGVVHDTYPQALIAREAEFPTGIMLEQHAVAIPHCEAIHAKSPAIYLIRPDK 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446546324  81 AIPVNRADEDGILWVRLVVNLIVTDPADQLRLLKPLFNHLQNEEFYQNLLTLPVDDAKALFVSKII 146
Cdd:PRK09665  81 KVHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLLTLPETQLKEYFTKYVL 146
 
Name Accession Description Interval E-value
PRK09665 PRK09665
PTS galactitol transporter subunit IIA;
1-146 7.14e-41

PTS galactitol transporter subunit IIA;


Pssm-ID: 182023 [Multi-domain]  Cd Length: 150  Bit Score: 133.77  E-value: 7.14e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446546324   1 MISISVADGAGLQNYDAVLEHIFNTHRTTNTVKESWLNAMRKREAEYPTGIELEGYSIAIPHCDSEHANQPAIYIIRLPD 80
Cdd:PRK09665   1 MSNLFVRTGISFVDRSEALTHIGNEMLAKGVVHDTYPQALIAREAEFPTGIMLEQHAVAIPHCEAIHAKSPAIYLIRPDK 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446546324  81 AIPVNRADEDGILWVRLVVNLIVTDPADQLRLLKPLFNHLQNEEFYQNLLTLPVDDAKALFVSKII 146
Cdd:PRK09665  81 KVHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLLTLPETQLKEYFTKYVL 146
PTS_IIA_fru cd00211
PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: ...
 32-141 5.79e-21

PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. This family is one of four structurally and functionally distinct group IIA PTS system cytoplasmic enzymes, necessary for the uptake of carbohydrates across the cytoplasmic membrane and their phosphorylation.


Pssm-ID: 238129 [Multi-domain]  Cd Length: 136  Bit Score: 82.22  E-value: 5.79e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446546324  32 VKESWLNAMRKREAEYPTGIeleGYSIAIPHCDSEHANQPAIYIIRLPDaiPVNRADEDGILwVRLVVNLIVTDPADQLR 111
Cdd:cd00211   32 VEEEYIEALLEREKEGSTGI---GNGIAIPHAKSEAVKKPGIAVLRLKE--PVDFGSLDGQP-VHLIFLLAAPDSNEHLK 105

                         90       100       110
                 ....*....|....*....|....*....|.
gi 446546324 112 LLKPLFNHLQNEEFYQNLLTLP-VDDAKALF 141
Cdd:cd00211  106 ALSQLARLLSDEEFVEQLLNAQsKEEILALL 136
PtsN COG1762
Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type) [Carbohydrate ...
 33-141 2.00e-19

Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type) [Carbohydrate transport and metabolism, Signal transduction mechanisms];


Pssm-ID: 441368 [Multi-domain]  Cd Length: 150  Bit Score: 78.74  E-value: 2.00e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446546324  33 KESWLNAMRKREAEYPTGIeleGYSIAIPHCDSEHANQPAIYIIRLPDAIPVNRADEDGilwVRLVVNLIV--TDPADQL 110
Cdd:COG1762   41 KEEYLEALLEREELGSTGI---GPGIAIPHARPEGVKKPGIAVARLKEPVDFGAMDGEP---VDLVFLLAApeDDSEEHL 114

                         90       100       110
                 ....*....|....*....|....*....|.
gi 446546324 111 RLLKPLFNHLQNEEFYQNLLTlpVDDAKALF 141
Cdd:COG1762  115 KLLAELARLLSDEEFREKLLN--AKSPEEIL 143
PTS_EIIA_2 pfam00359
Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2;
12-131 1.31e-17

Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2;


Pssm-ID: 459780 [Multi-domain]  Cd Length: 139  Bit Score: 73.78  E-value: 1.31e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446546324   12 LQNYDAVLEHIFNTHRTTNTVKESWLNAMRKREAEYPTGIeleGYSIAIPHCDSEHANQPAIYIIRLPDAIPVNRADEDg 91
Cdd:pfam00359  13 AKSKEEAIEFLADKLVEAGYVEPAYLEAILEREKEGSTGI---GNGIAIPHARSEAVKKPGIAVLTLKEPVDFGSEDGK- 88

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 446546324   92 ilWVRLVVNLIVTDP-ADQLRLLKPLFNHLQNEEFYQNLLT 131
Cdd:pfam00359  89 --PVKLIFLLAAPDNeASHLKILSQLARLLQDEEFVEKLLK 127
fruA TIGR00848
PTS system, fructose subfamily, IIA component; 4.A.2 The PTS Fructose-Mannitol (Fru) Family ...
 19-129 4.71e-08

PTS system, fructose subfamily, IIA component; 4.A.2 The PTS Fructose-Mannitol (Fru) Family Bacterial PTS transporters transport and concomitantly phosphorylate their sugar substrates, and typically consist of multiple subunits or protein domains. The Fru family is a large and complex family which includes several sequenced fructose and mannitol-specific permeases as well as several putative PTS permeases of unknown specificities. The fructose permeases of this family phosphorylate fructose on the 1-position. Those of family 4.6 phosphorylate fructose on the 6-position. The Fru family PTS systems typically have 3 domains, IIA, IIB and IIC, which may be found as 1 or more proteins. The fructose and mannitol transporters form separate phylogenetic clusters in this family. This model is specific for the IIA domain of the fructose PTS transporters. Also similar to the Enzyme IIA Fru subunits of the PTS, but included in TIGR01419 rather than this model, is enzyme IIA Ntr (nitrogen), also called PtsN, found in E. coli and other organisms, which may play a solely regulatory role. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids, Signal transduction, PTS]


Pssm-ID: 273298 [Multi-domain]  Cd Length: 129  Bit Score: 48.43  E-value: 4.71e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446546324   19 LEHIFNTHRTTNTvKESWLNAMRKREAEYPTGIeleGYSIAIPHCDSEHANQPAIYIIRLpdAIPVNRADEDGILwVRLV 98
Cdd:TIGR00848  23 LANKLLENGYISD-TEEFLEDLLKREEEGTTGI---GDGVAIPHAKSAAVKQPFVAIARL--VKGVDWQSLDGKP-VKLI 95

                          90       100       110
                  ....*....|....*....|....*....|....
gi 446546324   99 VNLIVTDPA---DQLRLLKPLFNHLQNEEFYQNL 129
Cdd:TIGR00848  96 FLIAVPKDEagnTHLKALSQLARLLLNDEFRAKL 129
 
Name Accession Description Interval E-value
PRK09665 PRK09665
PTS galactitol transporter subunit IIA;
1-146 7.14e-41

PTS galactitol transporter subunit IIA;


Pssm-ID: 182023 [Multi-domain]  Cd Length: 150  Bit Score: 133.77  E-value: 7.14e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446546324   1 MISISVADGAGLQNYDAVLEHIFNTHRTTNTVKESWLNAMRKREAEYPTGIELEGYSIAIPHCDSEHANQPAIYIIRLPD 80
Cdd:PRK09665   1 MSNLFVRTGISFVDRSEALTHIGNEMLAKGVVHDTYPQALIAREAEFPTGIMLEQHAVAIPHCEAIHAKSPAIYLIRPDK 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446546324  81 AIPVNRADEDGILWVRLVVNLIVTDPADQLRLLKPLFNHLQNEEFYQNLLTLPVDDAKALFVSKII 146
Cdd:PRK09665  81 KVHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLLTLPETQLKEYFTKYVL 146
PTS_IIA_fru cd00211
PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: ...
 32-141 5.79e-21

PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. This family is one of four structurally and functionally distinct group IIA PTS system cytoplasmic enzymes, necessary for the uptake of carbohydrates across the cytoplasmic membrane and their phosphorylation.


Pssm-ID: 238129 [Multi-domain]  Cd Length: 136  Bit Score: 82.22  E-value: 5.79e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446546324  32 VKESWLNAMRKREAEYPTGIeleGYSIAIPHCDSEHANQPAIYIIRLPDaiPVNRADEDGILwVRLVVNLIVTDPADQLR 111
Cdd:cd00211   32 VEEEYIEALLEREKEGSTGI---GNGIAIPHAKSEAVKKPGIAVLRLKE--PVDFGSLDGQP-VHLIFLLAAPDSNEHLK 105

                         90       100       110
                 ....*....|....*....|....*....|.
gi 446546324 112 LLKPLFNHLQNEEFYQNLLTLP-VDDAKALF 141
Cdd:cd00211  106 ALSQLARLLSDEEFVEQLLNAQsKEEILALL 136
PtsN COG1762
Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type) [Carbohydrate ...
 33-141 2.00e-19

Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type) [Carbohydrate transport and metabolism, Signal transduction mechanisms];


Pssm-ID: 441368 [Multi-domain]  Cd Length: 150  Bit Score: 78.74  E-value: 2.00e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446546324  33 KESWLNAMRKREAEYPTGIeleGYSIAIPHCDSEHANQPAIYIIRLPDAIPVNRADEDGilwVRLVVNLIV--TDPADQL 110
Cdd:COG1762   41 KEEYLEALLEREELGSTGI---GPGIAIPHARPEGVKKPGIAVARLKEPVDFGAMDGEP---VDLVFLLAApeDDSEEHL 114

                         90       100       110
                 ....*....|....*....|....*....|.
gi 446546324 111 RLLKPLFNHLQNEEFYQNLLTlpVDDAKALF 141
Cdd:COG1762  115 KLLAELARLLSDEEFREKLLN--AKSPEEIL 143
PTS_EIIA_2 pfam00359
Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2;
12-131 1.31e-17

Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2;


Pssm-ID: 459780 [Multi-domain]  Cd Length: 139  Bit Score: 73.78  E-value: 1.31e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446546324   12 LQNYDAVLEHIFNTHRTTNTVKESWLNAMRKREAEYPTGIeleGYSIAIPHCDSEHANQPAIYIIRLPDAIPVNRADEDg 91
Cdd:pfam00359  13 AKSKEEAIEFLADKLVEAGYVEPAYLEAILEREKEGSTGI---GNGIAIPHARSEAVKKPGIAVLTLKEPVDFGSEDGK- 88

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 446546324   92 ilWVRLVVNLIVTDP-ADQLRLLKPLFNHLQNEEFYQNLLT 131
Cdd:pfam00359  89 --PVKLIFLLAAPDNeASHLKILSQLARLLQDEEFVEKLLK 127
fruA TIGR00848
PTS system, fructose subfamily, IIA component; 4.A.2 The PTS Fructose-Mannitol (Fru) Family ...
 19-129 4.71e-08

PTS system, fructose subfamily, IIA component; 4.A.2 The PTS Fructose-Mannitol (Fru) Family Bacterial PTS transporters transport and concomitantly phosphorylate their sugar substrates, and typically consist of multiple subunits or protein domains. The Fru family is a large and complex family which includes several sequenced fructose and mannitol-specific permeases as well as several putative PTS permeases of unknown specificities. The fructose permeases of this family phosphorylate fructose on the 1-position. Those of family 4.6 phosphorylate fructose on the 6-position. The Fru family PTS systems typically have 3 domains, IIA, IIB and IIC, which may be found as 1 or more proteins. The fructose and mannitol transporters form separate phylogenetic clusters in this family. This model is specific for the IIA domain of the fructose PTS transporters. Also similar to the Enzyme IIA Fru subunits of the PTS, but included in TIGR01419 rather than this model, is enzyme IIA Ntr (nitrogen), also called PtsN, found in E. coli and other organisms, which may play a solely regulatory role. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids, Signal transduction, PTS]


Pssm-ID: 273298 [Multi-domain]  Cd Length: 129  Bit Score: 48.43  E-value: 4.71e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446546324   19 LEHIFNTHRTTNTvKESWLNAMRKREAEYPTGIeleGYSIAIPHCDSEHANQPAIYIIRLpdAIPVNRADEDGILwVRLV 98
Cdd:TIGR00848  23 LANKLLENGYISD-TEEFLEDLLKREEEGTTGI---GDGVAIPHAKSAAVKQPFVAIARL--VKGVDWQSLDGKP-VKLI 95

                          90       100       110
                  ....*....|....*....|....*....|....
gi 446546324   99 VNLIVTDPA---DQLRLLKPLFNHLQNEEFYQNL 129
Cdd:TIGR00848  96 FLIAVPKDEagnTHLKALSQLARLLLNDEFRAKL 129
MtlA2 COG4668
Mannitol/fructose-specific phosphotransferase system, IIA domain [Carbohydrate transport and ...
 32-141 2.63e-05

Mannitol/fructose-specific phosphotransferase system, IIA domain [Carbohydrate transport and metabolism];


Pssm-ID: 443705 [Multi-domain]  Cd Length: 143  Bit Score: 41.30  E-value: 2.63e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446546324  32 VKESWLNAMRKREAEYPTGIeleGYSIAIPHC---DSEHANQPAIYIIRLPDAIPVNRADEdgilwVRLVVNL-IVTDpa 107
Cdd:COG4668   35 VEPEYIDAMLEREAQVSTYL---GNGIAIPHGtneAKDLVLKTGISVLQFPDGVDWGDGNT-----VYLVIGIaAKSD-- 104

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 446546324 108 DQLRLLKPLFNHLQNEEFYQNLLTLP-VDDAKALF 141
Cdd:COG4668  105 EHLEILRQLARVLSDEENVEKLAKATdAEEILALL 139
PRK09913 PRK09913
PTS fructose transporter subunit IIA;
34-129 7.42e-05

PTS fructose transporter subunit IIA;


Pssm-ID: 182141 [Multi-domain]  Cd Length: 148  Bit Score: 40.25  E-value: 7.42e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446546324  34 ESWLNAMRKREAEYPTGIeleGYSIAIPHCDSEHANQPAIYIIRLPDAIPVNRAD-EDGILWVRLVVnlivtdPA----D 108
Cdd:PRK09913  39 HQFLQTLLLREKMHSTGF---GSGVAVPHGKSACVKQPFVLFARKAQAIDWQASDgEDVNCWICLGV------PQsgeeD 109

                         90       100
                 ....*....|....*....|.
gi 446546324 109 QLRLLKPLFNHLQNEEFYQNL 129
Cdd:PRK09913 110 QVKIIGTLCRKIIHQDFIHQL 130
PRK09765 PRK09765
PTS system 2-O-a-mannosyl-D-glycerate specific transporter subunit IIABC; Provisional
 33-141 9.55e-03

PTS system 2-O-a-mannosyl-D-glycerate specific transporter subunit IIABC; Provisional


Pssm-ID: 182066 [Multi-domain]  Cd Length: 631  Bit Score: 35.09  E-value: 9.55e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446546324  33 KESWLNAMRKREAEYPTGIElEGysIAIPHCDSEHANQPAIYIIRLPDAIPVNRADEDGIlwVRLVVNLIVtdPADQ--- 109
Cdd:PRK09765  41 TEQFLEEVYRRESLGPTALG-EG--LAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEA--VDLIFLLAI--PPNEagt 113

                         90       100       110
                 ....*....|....*....|....*....|....
gi 446546324 110 --LRLLKPLFNHLQNEEFYQNLLTlpVDDAKALF 141
Cdd:PRK09765 114 thMQLLTALTTRLADDEIRARIQS--ATTPDELL 145
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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