NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|446512097|ref|WP_000589552|]
View 

MULTISPECIES: arsenate reductase family protein [Staphylococcus]

Protein Classification

arsenate reductase family protein( domain architecture ID 10122556)

arsenate reductase (ArsC) family protein similar to Bacillus subtilis uncharacterized protein YusI and Geobacillus kaustophilus ArsC3, which is involved in arsenate reduction

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
ArsC_like cd03036
Arsenate Reductase (ArsC) family, unknown subfamily; uncharacterized proteins containing a ...
 2-112 1.67e-56

Arsenate Reductase (ArsC) family, unknown subfamily; uncharacterized proteins containing a CXXC motif with similarity to thioredoxin (TRX)-fold arsenic reductases, ArsC. Proteins containing a redox active CXXC motif like TRX and glutaredoxin (GRX) function as protein disulfide oxidoreductases, altering the redox state of target proteins via the reversible oxidation of the active site dithiol. ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], using reducing equivalents derived from glutathione via GRX, through a single catalytic cysteine.


:

Pssm-ID: 239334  Cd Length: 111  Bit Score: 170.50  E-value: 1.67e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   2 IKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLKNKLQTLSEDEK 81
Cdd:cd03036    1 LKFYEYPKCSTCRKAKKWLDEHGVDYTAIDIVEEPPSKEELKKWLEKSGLPLKKFFNTSGKSYRELGLKDKLPSLSEEEA 80

                         90       100       110
                 ....*....|....*....|....*....|.
gi 446512097  82 LELLSSDGMLVKRPLAVMGDKITLGFKEDQY 112
Cdd:cd03036   81 LELLSSDGMLIKRPFVVDDDKVLVGFKEEEW 111
 
Name Accession Description Interval E-value
ArsC_like cd03036
Arsenate Reductase (ArsC) family, unknown subfamily; uncharacterized proteins containing a ...
 2-112 1.67e-56

Arsenate Reductase (ArsC) family, unknown subfamily; uncharacterized proteins containing a CXXC motif with similarity to thioredoxin (TRX)-fold arsenic reductases, ArsC. Proteins containing a redox active CXXC motif like TRX and glutaredoxin (GRX) function as protein disulfide oxidoreductases, altering the redox state of target proteins via the reversible oxidation of the active site dithiol. ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], using reducing equivalents derived from glutathione via GRX, through a single catalytic cysteine.


Pssm-ID: 239334  Cd Length: 111  Bit Score: 170.50  E-value: 1.67e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   2 IKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLKNKLQTLSEDEK 81
Cdd:cd03036    1 LKFYEYPKCSTCRKAKKWLDEHGVDYTAIDIVEEPPSKEELKKWLEKSGLPLKKFFNTSGKSYRELGLKDKLPSLSEEEA 80

                         90       100       110
                 ....*....|....*....|....*....|.
gi 446512097  82 LELLSSDGMLVKRPLAVMGDKITLGFKEDQY 112
Cdd:cd03036   81 LELLSSDGMLIKRPFVVDDDKVLVGFKEEEW 111
arsC_related TIGR01617
transcriptional regulator, Spx/MgsR family; This model represents a portion of the proteins ...
 2-117 4.96e-54

transcriptional regulator, Spx/MgsR family; This model represents a portion of the proteins within the larger set covered by pfam03960. That larger family includes a glutaredoxin-dependent arsenate reductase (TIGR00014). Characterized members of this family include Spx and MgsR from Bacillus subtili. Spx is a global regulator for response to thiol-specific oxidative stress. It interacts with RNA polymerase. MgsR (modulator of the general stress response, also called YqgZ) provides a second level of regulation for more than a third of the proteins in the B. subtilis general stress regulon controlled by Sigma-B. [Regulatory functions, DNA interactions]


Pssm-ID: 273720  Cd Length: 117  Bit Score: 164.53  E-value: 4.96e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097    2 IKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLKNKLQTLSEDEK 81
Cdd:TIGR01617   1 IKVYGSPNCTTCKKARRWLEANGIEYQFIDIGEDGPTREELLDILSLLEDGIDPLLNTRGQSYRALNTSNTFLDLSDKEA 80

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 446512097   82 LELLSSDGMLVKRPLAVMGDKIT-LGFKEDQYKETWL 117
Cdd:TIGR01617  81 LELLAEDPALLRRPLIVDTKNRLlIGFKSESIEEFKL 117
ArsC COG1393
Arsenate reductase or related protein, glutaredoxin family [Inorganic ion transport and ...
 1-116 7.39e-41

Arsenate reductase or related protein, glutaredoxin family [Inorganic ion transport and metabolism];


Pssm-ID: 441003  Cd Length: 115  Bit Score: 131.37  E-value: 7.39e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   1 MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLKNKlqTLSEDE 80
Cdd:COG1393    1 MITIYGNPNCSTSRKALAWLEEAGIEYEFIDYLKTPPTAEELKELLAKLGLGVEELLNTRGTTYRELGLKDK--ALSEEE 78

                         90       100       110
                 ....*....|....*....|....*....|....*.
gi 446512097  81 KLELLSSDGMLVKRPLAVMGDKITLGFKEDQYKETW 116
Cdd:COG1393   79 ALALMLEHPSLIKRPIVVTGDKALVGFPPEEVLALL 114
ArsC pfam03960
ArsC family; This family is related to glutaredoxins pfam00462.
 5-114 2.42e-30

ArsC family; This family is related to glutaredoxins pfam00462.


Pssm-ID: 427617  Cd Length: 109  Bit Score: 104.22  E-value: 2.42e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097    5 YQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLKNKlqTLSEDEKLEL 84
Cdd:pfam03960   1 YGSPNCSTCRKALAWLEEHGIEYQEIDYLETPPSKEELKDILAKLGDGVEALLNTRGTTYRELNLDKE--DLSEDELLEL 78

                          90       100       110
                  ....*....|....*....|....*....|
gi 446512097   85 LSSDGMLVKRPLAVMGDKITLGFKEDQYKE 114
Cdd:pfam03960  79 ILEHPSLIRRPIVVDGGKLLVGFNEEEIRA 108
PRK10853 PRK10853
putative reductase; Provisional
 1-114 2.89e-12

putative reductase; Provisional


Pssm-ID: 182780  Cd Length: 118  Bit Score: 58.52  E-value: 2.89e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   1 MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEinKLFNTHGAKYRELDLKNKLQTLSEDE 80
Cdd:PRK10853   1 MVTLYGIKNCDTIKKARRWLEAQGIDYRFHDYRVDGLDSELLQGFIDELGWE--ALLNTRGTTWRKLDETQRNAITDAAS 78

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 446512097  81 KLELLSSDGMLVKRPLAVMGDK-ITLGFKEDQYKE 114
Cdd:PRK10853  79 AAALMLEQPAIIKRPLLCAPGKpMLLGFSESSYQQ 113
ArsC_rel_Se_1 NF041106
ArsC family (seleno)protein; Weak hits to Pfam model PF03960 and to COG1393 suggest a ...
 5-112 4.50e-06

ArsC family (seleno)protein; Weak hits to Pfam model PF03960 and to COG1393 suggest a relationship to glutaredoxin-like arsenate reductase protein ArsC. This family contains significant numbers of selenoproteins.


Pssm-ID: 469030  Cd Length: 107  Bit Score: 42.15  E-value: 4.50e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   5 YQYKNCTTCKKAAKFLDEYGVSY-EPIDIVQHTPTINEFKTIIAntgvEINKLFNTHGAKYRELDLKNKlqTLSEDEKLE 83
Cdd:NF041106   5 YHRKGCTTCKKAQAFLEEHGIEVkETVDARKERLDPEEALALLK----GVDKIYAAKGKKVVEFDLKKD--KPDDEELLA 78

                         90       100       110
                 ....*....|....*....|....*....|
gi 446512097  84 -LLSSDGMLvKRPLAVMGDKITLGFKEDQY 112
Cdd:NF041106  79 lLIGPTGNL-RAPTLRIGKTLLVGFNEEMY 107
 
Name Accession Description Interval E-value
ArsC_like cd03036
Arsenate Reductase (ArsC) family, unknown subfamily; uncharacterized proteins containing a ...
 2-112 1.67e-56

Arsenate Reductase (ArsC) family, unknown subfamily; uncharacterized proteins containing a CXXC motif with similarity to thioredoxin (TRX)-fold arsenic reductases, ArsC. Proteins containing a redox active CXXC motif like TRX and glutaredoxin (GRX) function as protein disulfide oxidoreductases, altering the redox state of target proteins via the reversible oxidation of the active site dithiol. ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], using reducing equivalents derived from glutathione via GRX, through a single catalytic cysteine.


Pssm-ID: 239334  Cd Length: 111  Bit Score: 170.50  E-value: 1.67e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   2 IKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLKNKLQTLSEDEK 81
Cdd:cd03036    1 LKFYEYPKCSTCRKAKKWLDEHGVDYTAIDIVEEPPSKEELKKWLEKSGLPLKKFFNTSGKSYRELGLKDKLPSLSEEEA 80

                         90       100       110
                 ....*....|....*....|....*....|.
gi 446512097  82 LELLSSDGMLVKRPLAVMGDKITLGFKEDQY 112
Cdd:cd03036   81 LELLSSDGMLIKRPFVVDDDKVLVGFKEEEW 111
arsC_related TIGR01617
transcriptional regulator, Spx/MgsR family; This model represents a portion of the proteins ...
 2-117 4.96e-54

transcriptional regulator, Spx/MgsR family; This model represents a portion of the proteins within the larger set covered by pfam03960. That larger family includes a glutaredoxin-dependent arsenate reductase (TIGR00014). Characterized members of this family include Spx and MgsR from Bacillus subtili. Spx is a global regulator for response to thiol-specific oxidative stress. It interacts with RNA polymerase. MgsR (modulator of the general stress response, also called YqgZ) provides a second level of regulation for more than a third of the proteins in the B. subtilis general stress regulon controlled by Sigma-B. [Regulatory functions, DNA interactions]


Pssm-ID: 273720  Cd Length: 117  Bit Score: 164.53  E-value: 4.96e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097    2 IKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLKNKLQTLSEDEK 81
Cdd:TIGR01617   1 IKVYGSPNCTTCKKARRWLEANGIEYQFIDIGEDGPTREELLDILSLLEDGIDPLLNTRGQSYRALNTSNTFLDLSDKEA 80

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 446512097   82 LELLSSDGMLVKRPLAVMGDKIT-LGFKEDQYKETWL 117
Cdd:TIGR01617  81 LELLAEDPALLRRPLIVDTKNRLlIGFKSESIEEFKL 117
ArsC COG1393
Arsenate reductase or related protein, glutaredoxin family [Inorganic ion transport and ...
 1-116 7.39e-41

Arsenate reductase or related protein, glutaredoxin family [Inorganic ion transport and metabolism];


Pssm-ID: 441003  Cd Length: 115  Bit Score: 131.37  E-value: 7.39e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   1 MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLKNKlqTLSEDE 80
Cdd:COG1393    1 MITIYGNPNCSTSRKALAWLEEAGIEYEFIDYLKTPPTAEELKELLAKLGLGVEELLNTRGTTYRELGLKDK--ALSEEE 78

                         90       100       110
                 ....*....|....*....|....*....|....*.
gi 446512097  81 KLELLSSDGMLVKRPLAVMGDKITLGFKEDQYKETW 116
Cdd:COG1393   79 ALALMLEHPSLIKRPIVVTGDKALVGFPPEEVLALL 114
ArsC_family cd02977
Arsenate Reductase (ArsC) family; composed of TRX-fold arsenic reductases and similar proteins ...
 2-107 1.20e-39

Arsenate Reductase (ArsC) family; composed of TRX-fold arsenic reductases and similar proteins including the transcriptional regulator, Spx. ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], using reducing equivalents derived from glutathione (GSH) via glutaredoxin (GRX), through a single catalytic cysteine. This family of predominantly bacterial enzymes is unrelated to two other families of arsenate reductases which show similarity to low-molecular-weight acid phosphatases and phosphotyrosyl phosphatases. Spx is a general regulator that exerts negative and positive control over transcription initiation by binding to the C-terminal domain of the alpha subunit of RNA polymerase.


Pssm-ID: 239275  Cd Length: 105  Bit Score: 127.99  E-value: 1.20e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   2 IKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLKNKlQTLSEDEK 81
Cdd:cd02977    1 ITIYGNPNCSTSRKALAWLEEHGIEYEFIDYLKEPPTKEELKELLAKLGLGVEDLFNTRGTPYRKLGLADK-DELSDEEA 79

                         90       100
                 ....*....|....*....|....*.
gi 446512097  82 LELLSSDGMLVKRPLAVMGDKITLGF 107
Cdd:cd02977   80 LELMAEHPKLIKRPIVVDGDRLLVGF 105
ArsC pfam03960
ArsC family; This family is related to glutaredoxins pfam00462.
 5-114 2.42e-30

ArsC family; This family is related to glutaredoxins pfam00462.


Pssm-ID: 427617  Cd Length: 109  Bit Score: 104.22  E-value: 2.42e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097    5 YQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLKNKlqTLSEDEKLEL 84
Cdd:pfam03960   1 YGSPNCSTCRKALAWLEEHGIEYQEIDYLETPPSKEELKDILAKLGDGVEALLNTRGTTYRELNLDKE--DLSEDELLEL 78

                          90       100       110
                  ....*....|....*....|....*....|
gi 446512097   85 LSSDGMLVKRPLAVMGDKITLGFKEDQYKE 114
Cdd:pfam03960  79 ILEHPSLIRRPIVVDGGKLLVGFNEEEIRA 108
ArsC_Yffb cd03035
Arsenate Reductase (ArsC) family, Yffb subfamily; Yffb is an uncharacterized bacterial protein ...
 2-109 9.13e-20

Arsenate Reductase (ArsC) family, Yffb subfamily; Yffb is an uncharacterized bacterial protein encoded by the yffb gene, related to the thioredoxin-fold arsenic reductases, ArsC. The structure of Yffb and the conservation of the catalytic cysteine suggest that it is likely to function as a glutathione (GSH)-dependent thiol reductase. ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], using reducing equivalents derived from GSH via glutaredoxin, through a single catalytic cysteine.


Pssm-ID: 239333  Cd Length: 105  Bit Score: 77.25  E-value: 9.13e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   2 IKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEinKLFNTHGAKYRELDLKNKlQTLSEDEK 81
Cdd:cd03035    1 ITLYGIKNCDTVKKARKWLEARGVAYTFHDYRKDGLDAATLERWLAKVGWE--TLLNKRGTTWRKLDDAQK-AALDAAKA 77

                         90       100
                 ....*....|....*....|....*...
gi 446512097  82 LELLSSDGMLVKRPLAVMGDKITLGFKE 109
Cdd:cd03035   78 IALMLEHPSLIKRPVLETGGKVLVGFSE 105
ArsC_Spx cd03032
Arsenate Reductase (ArsC) family, Spx subfamily; Spx is a unique RNA polymerase (RNAP)-binding ...
 1-110 4.79e-19

Arsenate Reductase (ArsC) family, Spx subfamily; Spx is a unique RNA polymerase (RNAP)-binding protein present in bacilli and some mollicutes. It inhibits transcription by binding to the C-terminal domain of the alpha subunit of RNAP, disrupting complex formation between RNAP and certain transcriptional activator proteins like ResD and ComA. In response to oxidative stress, Spx can also activate transcription, making it a general regulator that exerts both positive and negative control over transcription initiation. Spx has been shown to exert redox-sensitive transcriptional control over genes like trxA (TRX) and trxB (TRX reductase), genes that function in thiol homeostasis. This redox-sensitive activity is dependent on the presence of a CXXC motif, present in some members of the Spx subfamily, that acts as a thiol/disulfide switch. Spx has also been shown to repress genes in a sulfate-dependent manner independent of the presence of the CXXC motif.


Pssm-ID: 239330  Cd Length: 115  Bit Score: 75.74  E-value: 4.79e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   1 MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLknKLQTLSEDE 80
Cdd:cd03032    1 MIKLYTSPSCSSCRKAKQWLEEHQIPFEERNLFKQPLTKEELKEILSLTENGVEDIISTRSKAFKNLNI--DIDELSLSE 78

                         90       100       110
                 ....*....|....*....|....*....|
gi 446512097  81 KLELLSSDGMLVKRPLAVMGDKITLGFKED 110
Cdd:cd03032   79 LIRLISEHPSLLRRPIIIDEKRLQIGYNED 108
ArsC_ArsC cd03034
Arsenate Reductase (ArsC) family, ArsC subfamily; arsenic reductases similar to that encoded ...
 2-106 7.82e-13

Arsenate Reductase (ArsC) family, ArsC subfamily; arsenic reductases similar to that encoded by arsC on the R733 plasmid of Escherichia coli. E. coli ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], the first step in the detoxification of arsenic, using reducing equivalents derived from glutathione (GSH) via glutaredoxin (GRX). ArsC contains a single catalytic cysteine, within a thioredoxin fold, that forms a covalent thiolate-As(V) intermediate, which is reduced by GRX through a mixed GSH-arsenate intermediate. This family of predominantly bacterial enzymes is unrelated to two other families of arsenate reductases which show similarity to low-molecular-weight acid phosphatases and phosphotyrosyl phosphatases.


Pssm-ID: 239332  Cd Length: 112  Bit Score: 59.91  E-value: 7.82e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   2 IKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLKNklQTLSEDEK 81
Cdd:cd03034    1 ITIYHNPRCSKSRNALALLEEAGIEPEIVEYLKTPPTAAELRELLAKLGISPRDLLRTKEAPYKELGLAD--PELSDEEL 78

                         90       100
                 ....*....|....*....|....*
gi 446512097  82 LELLSSDGMLVKRPLAVMGDKITLG 106
Cdd:cd03034   79 IDAMAAHPILIERPIVVTGDGAVLG 103
PRK10853 PRK10853
putative reductase; Provisional
 1-114 2.89e-12

putative reductase; Provisional


Pssm-ID: 182780  Cd Length: 118  Bit Score: 58.52  E-value: 2.89e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   1 MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEinKLFNTHGAKYRELDLKNKLQTLSEDE 80
Cdd:PRK10853   1 MVTLYGIKNCDTIKKARRWLEAQGIDYRFHDYRVDGLDSELLQGFIDELGWE--ALLNTRGTTWRKLDETQRNAITDAAS 78

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 446512097  81 KLELLSSDGMLVKRPLAVMGDK-ITLGFKEDQYKE 114
Cdd:PRK10853  79 AAALMLEQPAIIKRPLLCAPGKpMLLGFSESSYQQ 113
PRK12559 PRK12559
transcriptional regulator Spx; Provisional
 1-114 1.57e-10

transcriptional regulator Spx; Provisional


Pssm-ID: 79035  Cd Length: 131  Bit Score: 54.34  E-value: 1.57e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   1 MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLknKLQTLSEDE 80
Cdd:PRK12559   1 MVVLYTTASCASCRKAKAWLEENQIDYTEKNIVSNSMTVDELKSILRLTEEGATEIISTRSKTFQDLNI--NIEELSLNE 78

                         90       100       110
                 ....*....|....*....|....*....|....
gi 446512097  81 KLELLSSDGMLVKRPLAVMGDKITLGFKEDQYKE 114
Cdd:PRK12559  79 FYKLIIEHPLMLRRPIMLDEKRLQIGFNDEEIRK 112
spxA PRK13344
transcriptional regulator Spx; Reviewed
 1-110 1.14e-09

transcriptional regulator Spx; Reviewed


Pssm-ID: 183988  Cd Length: 132  Bit Score: 52.27  E-value: 1.14e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   1 MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLknKLQTLSEDE 80
Cdd:PRK13344   1 MIKIYTISSCTSCKKAKTWLNAHQLSYKEQNLGKEPLTKEEILAILTKTENGIESIVSSKNRYAKALDC--DIEELSVNE 78

                         90       100       110
                 ....*....|....*....|....*....|
gi 446512097  81 KLELLSSDGMLVKRPLAVMGDKITLGFKED 110
Cdd:PRK13344  79 VIDLIQENPRILKSPILIDDKRLQVGYKED 108
spxA PRK01655
transcriptional regulator Spx; Reviewed
 1-111 4.07e-09

transcriptional regulator Spx; Reviewed


Pssm-ID: 179316  Cd Length: 131  Bit Score: 50.46  E-value: 4.07e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   1 MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLknKLQTLSEDE 80
Cdd:PRK01655   1 MVTLFTSPSCTSCRKAKAWLEEHDIPFTERNIFSSPLTIDEIKQILRMTEDGTDEIISTRSKVFQKLNV--DVESLSLQD 78

                         90       100       110
                 ....*....|....*....|....*....|.
gi 446512097  81 KLELLSSDGMLVKRPLAVMGDKITLGFKEDQ 111
Cdd:PRK01655  79 LIKLISDNPGLLRRPIIIDEKRLQVGYNEDE 109
ArsC_rel_Se_1 NF041106
ArsC family (seleno)protein; Weak hits to Pfam model PF03960 and to COG1393 suggest a ...
 5-112 4.50e-06

ArsC family (seleno)protein; Weak hits to Pfam model PF03960 and to COG1393 suggest a relationship to glutaredoxin-like arsenate reductase protein ArsC. This family contains significant numbers of selenoproteins.


Pssm-ID: 469030  Cd Length: 107  Bit Score: 42.15  E-value: 4.50e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446512097   5 YQYKNCTTCKKAAKFLDEYGVSY-EPIDIVQHTPTINEFKTIIAntgvEINKLFNTHGAKYRELDLKNKlqTLSEDEKLE 83
Cdd:NF041106   5 YHRKGCTTCKKAQAFLEEHGIEVkETVDARKERLDPEEALALLK----GVDKIYAAKGKKVVEFDLKKD--KPDDEELLA 78

                         90       100       110
                 ....*....|....*....|....*....|
gi 446512097  84 -LLSSDGMLvKRPLAVMGDKITLGFKEDQY 112
Cdd:NF041106  79 lLIGPTGNL-RAPTLRIGKTLLVGFNEEMY 107
GrxC COG0695
Glutaredoxin [Posttranslational modification, protein turnover, chaperones];
1-51 2.61e-05

Glutaredoxin [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440459 [Multi-domain]  Cd Length: 74  Bit Score: 39.41  E-value: 2.61e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 446512097   1 MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGV 51
Cdd:COG0695    1 KVTLYTTPGCPYCARAKRLLDEKGIPYEEIDVDEDPEAREELRERSGRRTV 51
NrdH cd02976
NrdH-redoxin (NrdH) family; NrdH is a small monomeric protein with a conserved redox active ...
 1-51 2.21e-04

NrdH-redoxin (NrdH) family; NrdH is a small monomeric protein with a conserved redox active CXXC motif within a TRX fold, characterized by a glutaredoxin (GRX)-like sequence and TRX-like activity profile. In vitro, it displays protein disulfide reductase activity that is dependent on TRX reductase, not glutathione (GSH). It is part of the NrdHIEF operon, where NrdEF codes for class Ib ribonucleotide reductase (RNR-Ib), an efficient enzyme at low oxygen levels. Under these conditions when GSH is mostly conjugated to spermidine, NrdH can still function and act as a hydrogen donor for RNR-Ib. It has been suggested that the NrdHEF system may be the oldest RNR reducing system, capable of functioning in a microaerophilic environment, where GSH was not yet available. NrdH from Corynebacterium ammoniagenes can form domain-swapped dimers, although it is unknown if this happens in vivo. Domain-swapped dimerization, which results in the blocking of the TRX reductase binding site, could be a mechanism for regulating the oxidation state of the protein.


Pssm-ID: 239274 [Multi-domain]  Cd Length: 73  Bit Score: 36.82  E-value: 2.21e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 446512097   1 MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGV 51
Cdd:cd02976    1 EVTVYTKPDCPYCKATKRFLDERGIPFEEVDVDEDPEALEELKKLNGYRSV 51
GST_N_mPGES2 cd03040
GST_N family; microsomal Prostaglandin E synthase Type 2 (mPGES2) subfamily; mPGES2 is a ...
 2-32 5.17e-04

GST_N family; microsomal Prostaglandin E synthase Type 2 (mPGES2) subfamily; mPGES2 is a membrane-anchored dimeric protein containing a CXXC motif which catalyzes the isomerization of PGH2 to PGE2. Unlike cytosolic PGE synthase (cPGES) and microsomal PGES Type 1 (mPGES1), mPGES2 does not require glutathione (GSH) for its activity, although its catalytic rate is increased two- to four-fold in the presence of DTT, GSH or other thiol compounds. PGE2 is widely distributed in various tissues and is implicated in the sleep/wake cycle, relaxation/contraction of smooth muscle, excretion of sodium ions, maintenance of body temperature and mediation of inflammation. mPGES2 contains an N-terminal hydrophobic domain which is membrane associated, and a C-terminal soluble domain with a GST-like structure.


Pssm-ID: 239338  Cd Length: 77  Bit Score: 36.23  E-value: 5.17e-04
                         10        20        30
                 ....*....|....*....|....*....|.
gi 446512097   2 IKFYQYKNCTTCKKAAKFLDEYGVSYEPIDI 32
Cdd:cd03040    2 ITLYQYKTCPFCCKVRAFLDYHGIPYEVVEV 32
Glutaredoxin pfam00462
Glutaredoxin;
2-35 5.88e-04

Glutaredoxin;


Pssm-ID: 425695 [Multi-domain]  Cd Length: 60  Bit Score: 35.56  E-value: 5.88e-04
                          10        20        30
                  ....*....|....*....|....*....|....
gi 446512097    2 IKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQH 35
Cdd:pfam00462   1 VVLYTKPTCPFCKRAKRLLKSLGVDFEEIDVDED 34
GRX_GRXb_1_3_like cd03418
Glutaredoxin (GRX) family, GRX bacterial class 1 and 3 (b_1_3)-like subfamily; composed of ...
 2-32 7.39e-03

Glutaredoxin (GRX) family, GRX bacterial class 1 and 3 (b_1_3)-like subfamily; composed of bacterial GRXs, approximately 10 kDa in size, and proteins containing a GRX or GRX-like domain. GRX is a glutathione (GSH) dependent reductase, catalyzing the disulfide reduction of target proteins such as ribonucleotide reductase. It contains a redox active CXXC motif in a TRX fold and uses a similar dithiol mechanism employed by TRXs for intramolecular disulfide bond reduction of protein substrates. Unlike TRX, GRX has preference for mixed GSH disulfide substrates, in which it uses a monothiol mechanism where only the N-terminal cysteine is required. The flow of reducing equivalents in the GRX system goes from NADPH -> GSH reductase -> GSH -> GRX -> protein substrates. By altering the redox state of target proteins, GRX is involved in many cellular functions including DNA synthesis, signal transduction and the defense against oxidative stress. Different classes are known including E. coli GRX1 and GRX3, which are members of this subfamily.


Pssm-ID: 239510 [Multi-domain]  Cd Length: 75  Bit Score: 32.95  E-value: 7.39e-03
                         10        20        30
                 ....*....|....*....|....*....|.
gi 446512097   2 IKFYQYKNCTTCKKAAKFLDEYGVSYEPIDI 32
Cdd:cd03418    2 VEIYTKPNCPYCVRAKALLDKKGVDYEEIDV 32
GlrX_YruB TIGR02196
Glutaredoxin-like protein, YruB-family; This glutaredoxin-like protein family contains the ...
 1-32 8.77e-03

Glutaredoxin-like protein, YruB-family; This glutaredoxin-like protein family contains the conserved CxxC motif and includes the Clostridium pasteurianum protein YruB which has been cloned from a rubredoxin operon. Somewhat related to NrdH, it is unknown whether this protein actually interacts with glutathione/glutathione reducatase, or, like NrdH, some other reductant system.


Pssm-ID: 274027 [Multi-domain]  Cd Length: 74  Bit Score: 32.74  E-value: 8.77e-03
                          10        20        30
                  ....*....|....*....|....*....|..
gi 446512097    1 MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDI 32
Cdd:TIGR02196   1 KVKVYTTPWCPPCVKAKEYLTSKGVAFEEIDV 32
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH