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Conserved domains on  [gi|446300102|ref|WP_000377957|]
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VasL domain-containing protein [Escherichia coli]

Protein Classification

TssA family type VI secretion system protein( domain architecture ID 10536196)

TssA family type VI secretion system protein functions in protein translocation and is involved in the pathogenicity of bacteria

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ImpA_N pfam06812
ImpA, N-terminal, type VI secretion system; This family represents a conserved region located ...
 12-113 4.42e-24

ImpA, N-terminal, type VI secretion system; This family represents a conserved region located towards the N-terminal end of ImpA and related proteins. ImpA is an inner membrane protein, which has been suggested to be involved with proteins that are exported and associated with colony variations in Actinobacillus actinomycetemcomitans. The ImpA gene in Vibrio cholera and many other bacteria is expressed from the virulence factor operon which produces the pathogenic injection, type VI secretion system; although the exact function of this gene-product is not known it appears to be an essential component of the pathogenic effect.


:

Pssm-ID: 429132 [Multi-domain]  Cd Length: 115  Bit Score: 96.53  E-value: 4.42e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446300102   12 TGSDPRGLPEFSAIREEINKASHPSQPELNWKLVESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVS 91
Cdd:pfam06812   9 CGEDLRYDPEFDALEEARNKADELAAKAVDWAKVERLALELLETRSKDLRVAAWLTEALIRLEGLAGLAEGLELLAGLLE 88

                          90       100
                  ....*....|....*....|....*.
gi 446300102   92 HDWDKFWPQGGP----ARTEMLDWFN 113
Cdd:pfam06812  89 RYWDTLHPALDDddleARVNALAWLN 114
VasL super family cl13867
Type VI secretion system, EvfB, or VasL; EvfB or VasL is a domain found on many Gram-negative ...
 292-435 1.59e-21

Type VI secretion system, EvfB, or VasL; EvfB or VasL is a domain found on many Gram-negative proteins with an ImpA_N domain at the N-terminus. These proteins are expressed from the pathogenicity locus that forms the bacterial type VI secretion system. The exact function of VasL is not known. One E.coli member is annotated as being EvfB, though the E.coli equivalent of ImpA would be expected to be EvfG. It is possible that in many bacteria what is a single protein in one species, eg E.coli, is a fusion of two genes in others, which would explain an ImpA at the N-terminus and a VasL at the C-terminus.


The actual alignment was detected with superfamily member pfam12486:

Pssm-ID: 463603  Cd Length: 147  Bit Score: 90.56  E-value: 1.59e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446300102  292 YERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQWNEALKTRAQSSPQLRGWLQTRQDLHAFAD--LVMQR 369
Cdd:pfam12486   1 YQQQLERLANLSPLWALSYGSQLVNQALQLWPDSPAVKALAQQWQQQLEARAGPAESLSGYHQARARLQQLADklLELER 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446300102  370 EKEGLTLSYIKNVIWQAERGLGQETPVESLLTQYQDARAQKQN-TDALEKQINERLEGVLSRWLLLK 435
Cdd:pfam12486  81 QRGKLTISYLKSAVYDIQQALNRDIPLEEQLRQLAARKASGQPvSPALLKQIEDRLNALLIRYYLLQ 147
 
Name Accession Description Interval E-value
ImpA_N pfam06812
ImpA, N-terminal, type VI secretion system; This family represents a conserved region located ...
 12-113 4.42e-24

ImpA, N-terminal, type VI secretion system; This family represents a conserved region located towards the N-terminal end of ImpA and related proteins. ImpA is an inner membrane protein, which has been suggested to be involved with proteins that are exported and associated with colony variations in Actinobacillus actinomycetemcomitans. The ImpA gene in Vibrio cholera and many other bacteria is expressed from the virulence factor operon which produces the pathogenic injection, type VI secretion system; although the exact function of this gene-product is not known it appears to be an essential component of the pathogenic effect.


Pssm-ID: 429132 [Multi-domain]  Cd Length: 115  Bit Score: 96.53  E-value: 4.42e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446300102   12 TGSDPRGLPEFSAIREEINKASHPSQPELNWKLVESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVS 91
Cdd:pfam06812   9 CGEDLRYDPEFDALEEARNKADELAAKAVDWAKVERLALELLETRSKDLRVAAWLTEALIRLEGLAGLAEGLELLAGLLE 88

                          90       100
                  ....*....|....*....|....*.
gi 446300102   92 HDWDKFWPQGGP----ARTEMLDWFN 113
Cdd:pfam06812  89 RYWDTLHPALDDddleARVNALAWLN 114
VasL pfam12486
Type VI secretion system, EvfB, or VasL; EvfB or VasL is a domain found on many Gram-negative ...
 292-435 1.59e-21

Type VI secretion system, EvfB, or VasL; EvfB or VasL is a domain found on many Gram-negative proteins with an ImpA_N domain at the N-terminus. These proteins are expressed from the pathogenicity locus that forms the bacterial type VI secretion system. The exact function of VasL is not known. One E.coli member is annotated as being EvfB, though the E.coli equivalent of ImpA would be expected to be EvfG. It is possible that in many bacteria what is a single protein in one species, eg E.coli, is a fusion of two genes in others, which would explain an ImpA at the N-terminus and a VasL at the C-terminus.


Pssm-ID: 463603  Cd Length: 147  Bit Score: 90.56  E-value: 1.59e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446300102  292 YERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQWNEALKTRAQSSPQLRGWLQTRQDLHAFAD--LVMQR 369
Cdd:pfam12486   1 YQQQLERLANLSPLWALSYGSQLVNQALQLWPDSPAVKALAQQWQQQLEARAGPAESLSGYHQARARLQQLADklLELER 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446300102  370 EKEGLTLSYIKNVIWQAERGLGQETPVESLLTQYQDARAQKQN-TDALEKQINERLEGVLSRWLLLK 435
Cdd:pfam12486  81 QRGKLTISYLKSAVYDIQQALNRDIPLEEQLRQLAARKASGQPvSPALLKQIEDRLNALLIRYYLLQ 147
TssA1 COG3515
Type VI protein secretion system component TssA1/VasJ/EvfE, contains ImpA_N domain ...
 12-136 3.18e-14

Type VI protein secretion system component TssA1/VasJ/EvfE, contains ImpA_N domain [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 442737 [Multi-domain]  Cd Length: 342  Bit Score: 73.51  E-value: 3.18e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446300102  12 TGSDPRGLPEFSAIREEINKASHPSQPE-------LNWKLVESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAE 84
Cdd:COG3515   16 CGEDLRYDPDFDALKEARRGDDEQQQGDwvielkpADWREVERLAEELLTTRSKDLRVAVWLAEALLRLDGLAGLADGLE 95

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 446300102  85 LLAAMVSHDWDKFWPQ---GGP-ARTEMLDWFNSRTGNI--LRQQISFAESDLPLIYR 136
Cdd:COG3515   96 LLAGLLERFWDDLHPQldeDDPeERVNALAWLNDPRLLLapLRLAPLTEGRGGELSLR 153
 
Name Accession Description Interval E-value
ImpA_N pfam06812
ImpA, N-terminal, type VI secretion system; This family represents a conserved region located ...
 12-113 4.42e-24

ImpA, N-terminal, type VI secretion system; This family represents a conserved region located towards the N-terminal end of ImpA and related proteins. ImpA is an inner membrane protein, which has been suggested to be involved with proteins that are exported and associated with colony variations in Actinobacillus actinomycetemcomitans. The ImpA gene in Vibrio cholera and many other bacteria is expressed from the virulence factor operon which produces the pathogenic injection, type VI secretion system; although the exact function of this gene-product is not known it appears to be an essential component of the pathogenic effect.


Pssm-ID: 429132 [Multi-domain]  Cd Length: 115  Bit Score: 96.53  E-value: 4.42e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446300102   12 TGSDPRGLPEFSAIREEINKASHPSQPELNWKLVESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVS 91
Cdd:pfam06812   9 CGEDLRYDPEFDALEEARNKADELAAKAVDWAKVERLALELLETRSKDLRVAAWLTEALIRLEGLAGLAEGLELLAGLLE 88

                          90       100
                  ....*....|....*....|....*.
gi 446300102   92 HDWDKFWPQGGP----ARTEMLDWFN 113
Cdd:pfam06812  89 RYWDTLHPALDDddleARVNALAWLN 114
VasL pfam12486
Type VI secretion system, EvfB, or VasL; EvfB or VasL is a domain found on many Gram-negative ...
 292-435 1.59e-21

Type VI secretion system, EvfB, or VasL; EvfB or VasL is a domain found on many Gram-negative proteins with an ImpA_N domain at the N-terminus. These proteins are expressed from the pathogenicity locus that forms the bacterial type VI secretion system. The exact function of VasL is not known. One E.coli member is annotated as being EvfB, though the E.coli equivalent of ImpA would be expected to be EvfG. It is possible that in many bacteria what is a single protein in one species, eg E.coli, is a fusion of two genes in others, which would explain an ImpA at the N-terminus and a VasL at the C-terminus.


Pssm-ID: 463603  Cd Length: 147  Bit Score: 90.56  E-value: 1.59e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446300102  292 YERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQWNEALKTRAQSSPQLRGWLQTRQDLHAFAD--LVMQR 369
Cdd:pfam12486   1 YQQQLERLANLSPLWALSYGSQLVNQALQLWPDSPAVKALAQQWQQQLEARAGPAESLSGYHQARARLQQLADklLELER 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446300102  370 EKEGLTLSYIKNVIWQAERGLGQETPVESLLTQYQDARAQKQN-TDALEKQINERLEGVLSRWLLLK 435
Cdd:pfam12486  81 QRGKLTISYLKSAVYDIQQALNRDIPLEEQLRQLAARKASGQPvSPALLKQIEDRLNALLIRYYLLQ 147
TssA1 COG3515
Type VI protein secretion system component TssA1/VasJ/EvfE, contains ImpA_N domain ...
 12-136 3.18e-14

Type VI protein secretion system component TssA1/VasJ/EvfE, contains ImpA_N domain [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 442737 [Multi-domain]  Cd Length: 342  Bit Score: 73.51  E-value: 3.18e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446300102  12 TGSDPRGLPEFSAIREEINKASHPSQPE-------LNWKLVESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAE 84
Cdd:COG3515   16 CGEDLRYDPDFDALKEARRGDDEQQQGDwvielkpADWREVERLAEELLTTRSKDLRVAVWLAEALLRLDGLAGLADGLE 95

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 446300102  85 LLAAMVSHDWDKFWPQ---GGP-ARTEMLDWFNSRTGNI--LRQQISFAESDLPLIYR 136
Cdd:COG3515   96 LLAGLLERFWDDLHPQldeDDPeERVNALAWLNDPRLLLapLRLAPLTEGRGGELSLR 153
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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