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Conserved domains on  [gi|446131504|ref|WP_000209359|]
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MULTISPECIES: glycyl radical protein [Enterobacteriaceae]

Protein Classification

glycyl radical protein( domain architecture ID 11493154)

glycyl radical protein similar to formate acetyltransferase, benzylsuccinate synthase, 4-hydroxyphenylacetate decarboxylase, and CutC choline trimethylamine-lyase

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
PFL2-3 TIGR01774
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
8-810 0e+00

glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.


:

Pssm-ID: 273798 [Multi-domain]  Cd Length: 786  Bit Score: 1404.65  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504    8 TLSDRIKAHKNALVHIVkPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIF 87
Cdd:TIGR01774   1 MCMDRIEKLKKALSKPV-ARLSVERARLYTESMKQTEGEPMIIRRAKALAHVLENIPIQILDSELIVGNMLPNPRAAIIF 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504   88 PEYTVSWIEKEIDDLADRPGAGFAVSEENKRVLH-EVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGnMTSGDAHL 166
Cdd:TIGR01774  80 PEGVGLWIIKELDSLPNRPTNRFAVSEEDKRVLReEIAPYWQRKTIEDFAFGLMTDEMKILYTTSIFVLTG-EIAGISHV 158
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  167 AVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRRDELLTM 246
Cdd:TIGR01774 159 AVNYPYLLRRGLRWLLEESERRIRALEESGVYEGEKYSFYQAAKIVLEAVINHILRYAKLAEEMAASETGESRREELLKI 238
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  247 AENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQtLDREHAIEMLHSCWLKLLEV 326
Cdd:TIGR01774 239 AEICRKVAAEKPQTFWQAVQLVWLVQSILQQESNEQSISMGRIDQYLYPFYKKDIGEGR-IDRELAFEILASLWIKTNEI 317
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  327 NKIRSGSHSKASAGSPLYQNVTIGGQnlvDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRC 406
Cdd:TIGR01774 318 VPARSSSLEQYFAGQPTNQAVTIGGC---DIYGNDAVNELSYLMLEVTDRLRLRQPNLHVRINAGSPESFLKRLAEAIRS 394
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  407 GFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWgYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQE 486
Cdd:TIGR01774 395 GCGNPALFNDEAVVPAFKNAGVDDRDALDYTTDGCVEIAPFGNS-YTSSDAALINVAKVMEYALNEGVDLQFGYEFGAKT 473
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  487 KALsagnfNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI 566
Cdd:TIGR01774 474 EKP-----KFLEDLLEKLREQISHYVKLVVEGSNVLDYANAEVKPTPLLSLCVEDCFEKGVDISRGGARYNFTGIQAVGI 548
                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  567 ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHNP 646
Cdd:TIGR01774 549 ANLGDSLVAIKGAVFEQGAYSMDDLVEALRKNFVGLTHEEKRHRLINQSPKYGNDDDAVDKYTAMVLEWYCEEVNRYRNP 628
                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  647 RygrgpvGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQK 726
Cdd:TIGR01774 629 R------GGKFAAGCYPMSANVPFGFFTSATPDGRKAGEPLAPGVSPSTGMDREGPTAVINSVSKLSYENLPNGALLNIK 702
                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  727 LNPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDDIIART 806
Cdd:TIGR01774 703 LSPATLEEEGDKQKLIEALRKSFMELKVMHIQFNIVKEDTLRKAQQEPEKYRWLVVRVAGYSAFFVELSRPVQDDIIRRT 782

                  ....
gi 446131504  807 EHML 810
Cdd:TIGR01774 783 SHRL 786
 
Name Accession Description Interval E-value
PFL2-3 TIGR01774
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
8-810 0e+00

glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.


Pssm-ID: 273798 [Multi-domain]  Cd Length: 786  Bit Score: 1404.65  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504    8 TLSDRIKAHKNALVHIVkPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIF 87
Cdd:TIGR01774   1 MCMDRIEKLKKALSKPV-ARLSVERARLYTESMKQTEGEPMIIRRAKALAHVLENIPIQILDSELIVGNMLPNPRAAIIF 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504   88 PEYTVSWIEKEIDDLADRPGAGFAVSEENKRVLH-EVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGnMTSGDAHL 166
Cdd:TIGR01774  80 PEGVGLWIIKELDSLPNRPTNRFAVSEEDKRVLReEIAPYWQRKTIEDFAFGLMTDEMKILYTTSIFVLTG-EIAGISHV 158
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  167 AVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRRDELLTM 246
Cdd:TIGR01774 159 AVNYPYLLRRGLRWLLEESERRIRALEESGVYEGEKYSFYQAAKIVLEAVINHILRYAKLAEEMAASETGESRREELLKI 238
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  247 AENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQtLDREHAIEMLHSCWLKLLEV 326
Cdd:TIGR01774 239 AEICRKVAAEKPQTFWQAVQLVWLVQSILQQESNEQSISMGRIDQYLYPFYKKDIGEGR-IDRELAFEILASLWIKTNEI 317
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  327 NKIRSGSHSKASAGSPLYQNVTIGGQnlvDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRC 406
Cdd:TIGR01774 318 VPARSSSLEQYFAGQPTNQAVTIGGC---DIYGNDAVNELSYLMLEVTDRLRLRQPNLHVRINAGSPESFLKRLAEAIRS 394
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  407 GFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWgYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQE 486
Cdd:TIGR01774 395 GCGNPALFNDEAVVPAFKNAGVDDRDALDYTTDGCVEIAPFGNS-YTSSDAALINVAKVMEYALNEGVDLQFGYEFGAKT 473
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  487 KALsagnfNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI 566
Cdd:TIGR01774 474 EKP-----KFLEDLLEKLREQISHYVKLVVEGSNVLDYANAEVKPTPLLSLCVEDCFEKGVDISRGGARYNFTGIQAVGI 548
                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  567 ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHNP 646
Cdd:TIGR01774 549 ANLGDSLVAIKGAVFEQGAYSMDDLVEALRKNFVGLTHEEKRHRLINQSPKYGNDDDAVDKYTAMVLEWYCEEVNRYRNP 628
                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  647 RygrgpvGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQK 726
Cdd:TIGR01774 629 R------GGKFAAGCYPMSANVPFGFFTSATPDGRKAGEPLAPGVSPSTGMDREGPTAVINSVSKLSYENLPNGALLNIK 702
                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  727 LNPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDDIIART 806
Cdd:TIGR01774 703 LSPATLEEEGDKQKLIEALRKSFMELKVMHIQFNIVKEDTLRKAQQEPEKYRWLVVRVAGYSAFFVELSRPVQDDIIRRT 782

                  ....
gi 446131504  807 EHML 810
Cdd:TIGR01774 783 SHRL 786
PFL2_DhaB_BssA cd01677
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 ...
10-807 0e+00

Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 (PFL2), B12-independent glycerol dehydratase (DhaB) and the alpha subunit of benzylsuccinate synthase (BssA), all of which have a highly conserved ten-stranded alpha/beta barrel domain, which is similar to those of PFL1 (pyruvate formate lyase 1) and RNR (ribonucleotide reductase). Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. DhaB catalyzes the first step in the conversion of glycerol to 1,3-propanediol while BssA catalyzes the first step in the anaerobic mineralization of both toluene and m-xylene.


Pssm-ID: 153086 [Multi-domain]  Cd Length: 781  Bit Score: 1179.37  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  10 SDRIKAHKNALVHIvKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPE 89
Cdd:cd01677    1 TERIKRLKAKILTA-KPSISIERARLYTESYKETEGKPVIIRRAKALKHILENATIYIQDDELIVGNRGGKPRAVPVFPE 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  90 YTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMTSGDAHLAVN 169
Cdd:cd01677   80 LSVHWVEDELDDLPKRPGDPFVISEDKKEYLEEIFPYWKGKTLRDRCFKYFPEETLIAMAAGVFTEFMYFFSGPGHVAVD 159
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 170 FPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRRDELLTMAEN 249
Cdd:cd01677  160 YPKVLEKGLDGLIEEAKEAIEALDLTGPEDIDKIYFYQAMIIVCEAVITYAKRYAELAKELAAKETDPKRKAELLEIAEI 239
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 250 CDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQtLDREHAIEMLHSCWLKLLEVNKI 329
Cdd:cd01677  240 CRRVPAHPPRTFWEALQSFWFIHLILQIESNGHSISPGRFDQYLYPFYKQDIEEGR-LTREGAIELLECLWIKINEINKV 318
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 330 RSGSHSKASAGSPLYQNVTIGGQNLvDGQpmDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRCGFG 409
Cdd:cd01677  319 RSGASAKYFAGYNTFQNLTIGGQTE-DGS--DATNELSYLILEATRRVRLPQPSLTVRYHAKSPDKFLKKAAEVIRLGLG 395
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 410 MPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGkWGYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQekAL 489
Cdd:cd01677  396 YPAFFNDEVVIPALLRKGVSLEDARDYGLIGCVETGAPG-RKYRWTGTGYINLAKVLEITLNNGKDPRSGKQVGPE--TG 472
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 490 SAGNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGIANL 569
Cdd:cd01677  473 DATDFKTFEELWEAFKKQLRHFIKLSVRANNISDIAHAEVAPAPFLSALVDDCIEKGKDINAGGARYNFGGIQGVGIATL 552
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 570 GNSLAAVKKLVFEQGAIGQQQLAAALADDFDGltHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHNPRyg 649
Cdd:cd01677  553 GDSLAAIKKLVFEEKKLTMEELLEALKANFAE--GYEERRRLLNNAPKYGNDDDYADNIARRVYEWYCKEVEKYQNPR-- 628
                        650       660       670       680       690       700       710       720
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 650 rgpvGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNP 729
Cdd:cd01677  629 ----GGKFYPGTYSVSANVPFGSVTGATPDGRLAGTPLSDGVSPSQGTDKKGPTAVIKSVSKLDHFNISGGTLLNQKFSP 704
                        730       740       750       760       770       780       790
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446131504 730 ATLENESDKQKLMILLRTFFEVhKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDDIIARTE 807
Cdd:cd01677  705 STLEGEEGLKKLAALIRTYFDL-GGHHIQFNVVSAETLRDAQKHPEKYRDLIVRVAGYSAYFVELSKEVQDEIIARTE 781
PflD COG1882
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ...
6-808 0e+00

Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation


Pssm-ID: 441486 [Multi-domain]  Cd Length: 789  Bit Score: 1072.47  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504   6 LDTLSDRIKAHKNALVHiVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAP 85
Cdd:COG1882    4 LAGPTERTKRLREKLLE-AKPLIDIERARLFTESYKETEGLPVIIRRAKAFSHILEHKPIYIKDDELIVGLQTDKPRKRP 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  86 IFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKaEGNMTSGDAH 165
Cdd:COG1882   83 IFPEGGIRWVEDELDALPTRPQDGFEISPEDKEIFREIAPYWKGKTHNDGVFDAYPEEIRKARKAGIIT-GLPDAYGRGH 161
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 166 LAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRRDELLT 245
Cdd:COG1882  162 IIGDYRRVLLYGLDGLIEEAKEKLAELDLTDPEDIEKIDFYKAMIIVCEAVIRLAERYAELARELAEKETDPKRKAELLE 241
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 246 MAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVElNQTLDREHAIEMLHSCWLKLLE 325
Cdd:COG1882  242 IAEICGFVPANPARTFWEAVQWVWFVYLAAIKEQNGAAMSLGRFDQYLYPYYERDLE-EGRLTEEEAQELLDCFWIKLRE 320
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 326 VNKIRSGSHSKASAGSPLYQNVTIGGQNlVDGQpmDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIR 405
Cdd:COG1882  321 VRFLRTPEYAELFAGYPTWVTLTIGGMT-PDGR--DAVNELSYLILETLRNLPLPEPNLTVRWSEKLPEGFLKKAAEVIS 397
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 406 CGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWgYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQ 485
Cdd:COG1882  398 IGTGSPQYENDDLMIPMLLNKGVTLEDARDYGIAGCVEPMVPGKQ-MQFFGAGRINLAKALEYALNNGVDEKTGKQVGPE 476
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 486 EKALSagNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVG 565
Cdd:COG1882  477 TGDPT--DFLTYDEVMEAFKKQLDYLADLYVNALNIIHYMHDKYAPEPFLSALHDDCIERGKDLNEGGARYNFGAIGIAG 554
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 566 IANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGltHEQLRQRLINgAPKYGNDDDTVDTLLARAYQTYIDELKQYHN 645
Cdd:COG1882  555 LSVVADSLSAIKKLVFDKKKVTMDELLEALAANFEG--YEELRQLLLN-APKYGNDDDYVDEIAVELVETFMDEIRKYKT 631
                        650       660       670       680       690       700       710       720
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 646 PRygrgpvGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQ 725
Cdd:COG1882  632 YR------GGTYTLSILTITSNVPYGKKTGATPDGRKAGEPLADGASPMHGRDKNGPTAVLKSVAKLPYEKATDGILLNQ 705
                        730       740       750       760       770       780       790       800
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 726 KLNPATLENESDKQKLMILLRTFFEvHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDDIIAR 805
Cdd:COG1882  706 KFSPSALGGEEGIENLVSLLRTYFD-LGGHHIQFNVVDRETLLDAQKHPEKYPDLTVRVAGYSAYFVELSKEQQDDIIAR 784

                 ...
gi 446131504 806 TEH 808
Cdd:COG1882  785 TEH 787
PFL-like pfam02901
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ...
9-671 0e+00

Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.


Pssm-ID: 427048  Cd Length: 647  Bit Score: 747.15  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504    9 LSDRIKAHK-NALVHIVKPPVCTERAQHYTEMYQQHLDK-PIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPI 86
Cdd:pfam02901   1 MWERIDVLKeNYTLYTGDPSLSWERARLLTESYKETEGVlPVDIRRAKALKKILSHLPGYIRDDELIVGLQTDKPRKRAI 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504   87 FPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKaEGNMTSGDAHL 166
Cdd:pfam02901  81 YPEGGIRWVEDELDYLNTRPQDGFEISEEDKKIFREIFPYWKGKTHNEGVFDAYTPEMKAARESGIFT-GLPDAYGRGHI 159
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  167 AVNFPLLLEKGLDGLREKVAERRSRINlTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRRDELLTM 246
Cdd:pfam02901 160 IGDYRRVLLYGLDGLIEEKEEKLAKLD-TDPEDIEKIEFYKAMIISCDAVIEYAERYARLAEELAEQETDPKRKAELLEI 238
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  247 AENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVElNQTLDREHAIEMLHSCWLKLLEV 326
Cdd:pfam02901 239 AEICGRVPARPAETFQEAIQWFWFVYLAAVKEQNGAAMSLGRLDQYLYPYYERDLE-EGRLTEEEAQELIDCFWIKLREV 317
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  327 NKIRSGSHSKASAGSPLYQNVTIGGQNLvDGQpmDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRC 406
Cdd:pfam02901 318 RFLRTPEYNKLFAGYDPFQNLTIGGQGR-DGR--DAVNKLSYLILEALDNLPLPEPNLTVRWSKKLPEEFLKKAAEVSRK 394
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  407 GFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGK-WGYRCtgmSFINFARVMLAALEGGRDATSGKVFLPQ 485
Cdd:pfam02901 395 GTGSPQYENDDVMIPALLNRGVSLEDARDYGIAGCVEPMKPGKeMQFFG---ARINLAKALEYALNGGRDELTGKQVGPK 471
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  486 EKALSagNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVG 565
Cdd:pfam02901 472 TGPVT--EFLSFEEVMEAFKKQLDYLADLYVNALNIIHYMHDKYAPEPFLSALHDDCIERGKDVGIGGARYNFSGPQGAG 549
                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  566 IANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGltHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHN 645
Cdd:pfam02901 550 LANVADSLSAIKKLVFDDKVYTLRELEDALAADFEG--EEELRQDLLNDAPKYGNDDDRVDDIAVEVVETFMDEVRKYKN 627
                         650       660
                  ....*....|....*....|....*.
gi 446131504  646 PRygrgpvGGNYYAGTSSISANVPFG 671
Cdd:pfam02901 628 YR------GGKFTPSLLTITSNVPYG 647
pflD PRK09983
putative formate acetyltransferase 2; Provisional
9-810 0e+00

putative formate acetyltransferase 2; Provisional


Pssm-ID: 182181 [Multi-domain]  Cd Length: 765  Bit Score: 584.09  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504   9 LSDRIKAHKNALVHIVKPpVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFP 88
Cdd:PRK09983   1 MTNRISRLKTALFANTRE-ISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSP 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  89 EYTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVC-PWWRGQTVQDRCYGMFTDEQKGLLATGIIKAegNMT-SGDAHL 166
Cdd:PRK09983  80 EMDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELfPYWEKRSMKDFINGQMTDEVKAATSTQIFSI--NQTdKGQGHI 157
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 167 AVNFPLLLEKGLDGLREKVAERrsrinltvLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRRDELLTM 246
Cdd:PRK09983 158 IIDYPRLLNHGLGELVAQMQQH--------CQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTI 229
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 247 AENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDveLNQTLDREHAIEMLHSCWLKLLEV 326
Cdd:PRK09983 230 AEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQAS--LTQGEDPAFLKELLESLWVKCNDI 307
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 327 NKIRSGSHSKASAGSPLYQNVTIGGqnlVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRC 406
Cdd:PRK09983 308 VLLRSTSSARYFAGFPTGYTALLGG---LTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRL 384
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 407 GFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKwGYRCTGMSFINFARVMLAAL---EGGRDATsgkvfl 483
Cdd:PRK09983 385 GTGIPQIFNDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGR-TYGLHDIAMFNLLKVMEICLhenEGNAALT------ 457
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 484 pqekalsagnfnnFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWvSGLQ 563
Cdd:PRK09983 458 -------------YEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNF-SGVQ 523
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 564 -VGIANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQ 642
Cdd:PRK09983 524 gIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEK 603
                        650       660       670       680       690       700       710       720
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 643 YHNPRygrgpvGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEGA-SPASGTDHLGPTAVIGSVGKLPTAAILGGV 721
Cdd:PRK09983 604 YQNPR------GGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQLADGGlSPMLGQDAQGPTAVLKSVSKLDNTLLSNGT 677
                        730       740       750       760       770       780       790       800
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 722 LLNQKLNPATLENESDKQKLMILLRTFFEVhKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDD 801
Cdd:PRK09983 678 LLNVKFTPATLEGEAGLRKLADFLRAFTQL-KLQHIQFNVVNADTLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDD 756

                 ....*....
gi 446131504 802 IIARTEHML 810
Cdd:PRK09983 757 IIRRTAHQL 765
 
Name Accession Description Interval E-value
PFL2-3 TIGR01774
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
8-810 0e+00

glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.


Pssm-ID: 273798 [Multi-domain]  Cd Length: 786  Bit Score: 1404.65  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504    8 TLSDRIKAHKNALVHIVkPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIF 87
Cdd:TIGR01774   1 MCMDRIEKLKKALSKPV-ARLSVERARLYTESMKQTEGEPMIIRRAKALAHVLENIPIQILDSELIVGNMLPNPRAAIIF 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504   88 PEYTVSWIEKEIDDLADRPGAGFAVSEENKRVLH-EVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGnMTSGDAHL 166
Cdd:TIGR01774  80 PEGVGLWIIKELDSLPNRPTNRFAVSEEDKRVLReEIAPYWQRKTIEDFAFGLMTDEMKILYTTSIFVLTG-EIAGISHV 158
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  167 AVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRRDELLTM 246
Cdd:TIGR01774 159 AVNYPYLLRRGLRWLLEESERRIRALEESGVYEGEKYSFYQAAKIVLEAVINHILRYAKLAEEMAASETGESRREELLKI 238
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  247 AENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQtLDREHAIEMLHSCWLKLLEV 326
Cdd:TIGR01774 239 AEICRKVAAEKPQTFWQAVQLVWLVQSILQQESNEQSISMGRIDQYLYPFYKKDIGEGR-IDRELAFEILASLWIKTNEI 317
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  327 NKIRSGSHSKASAGSPLYQNVTIGGQnlvDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRC 406
Cdd:TIGR01774 318 VPARSSSLEQYFAGQPTNQAVTIGGC---DIYGNDAVNELSYLMLEVTDRLRLRQPNLHVRINAGSPESFLKRLAEAIRS 394
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  407 GFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWgYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQE 486
Cdd:TIGR01774 395 GCGNPALFNDEAVVPAFKNAGVDDRDALDYTTDGCVEIAPFGNS-YTSSDAALINVAKVMEYALNEGVDLQFGYEFGAKT 473
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  487 KALsagnfNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI 566
Cdd:TIGR01774 474 EKP-----KFLEDLLEKLREQISHYVKLVVEGSNVLDYANAEVKPTPLLSLCVEDCFEKGVDISRGGARYNFTGIQAVGI 548
                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  567 ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHNP 646
Cdd:TIGR01774 549 ANLGDSLVAIKGAVFEQGAYSMDDLVEALRKNFVGLTHEEKRHRLINQSPKYGNDDDAVDKYTAMVLEWYCEEVNRYRNP 628
                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  647 RygrgpvGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQK 726
Cdd:TIGR01774 629 R------GGKFAAGCYPMSANVPFGFFTSATPDGRKAGEPLAPGVSPSTGMDREGPTAVINSVSKLSYENLPNGALLNIK 702
                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  727 LNPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDDIIART 806
Cdd:TIGR01774 703 LSPATLEEEGDKQKLIEALRKSFMELKVMHIQFNIVKEDTLRKAQQEPEKYRWLVVRVAGYSAFFVELSRPVQDDIIRRT 782

                  ....
gi 446131504  807 EHML 810
Cdd:TIGR01774 783 SHRL 786
PFL2_DhaB_BssA cd01677
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 ...
10-807 0e+00

Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 (PFL2), B12-independent glycerol dehydratase (DhaB) and the alpha subunit of benzylsuccinate synthase (BssA), all of which have a highly conserved ten-stranded alpha/beta barrel domain, which is similar to those of PFL1 (pyruvate formate lyase 1) and RNR (ribonucleotide reductase). Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. DhaB catalyzes the first step in the conversion of glycerol to 1,3-propanediol while BssA catalyzes the first step in the anaerobic mineralization of both toluene and m-xylene.


Pssm-ID: 153086 [Multi-domain]  Cd Length: 781  Bit Score: 1179.37  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  10 SDRIKAHKNALVHIvKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPE 89
Cdd:cd01677    1 TERIKRLKAKILTA-KPSISIERARLYTESYKETEGKPVIIRRAKALKHILENATIYIQDDELIVGNRGGKPRAVPVFPE 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  90 YTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMTSGDAHLAVN 169
Cdd:cd01677   80 LSVHWVEDELDDLPKRPGDPFVISEDKKEYLEEIFPYWKGKTLRDRCFKYFPEETLIAMAAGVFTEFMYFFSGPGHVAVD 159
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 170 FPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRRDELLTMAEN 249
Cdd:cd01677  160 YPKVLEKGLDGLIEEAKEAIEALDLTGPEDIDKIYFYQAMIIVCEAVITYAKRYAELAKELAAKETDPKRKAELLEIAEI 239
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 250 CDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQtLDREHAIEMLHSCWLKLLEVNKI 329
Cdd:cd01677  240 CRRVPAHPPRTFWEALQSFWFIHLILQIESNGHSISPGRFDQYLYPFYKQDIEEGR-LTREGAIELLECLWIKINEINKV 318
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 330 RSGSHSKASAGSPLYQNVTIGGQNLvDGQpmDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRCGFG 409
Cdd:cd01677  319 RSGASAKYFAGYNTFQNLTIGGQTE-DGS--DATNELSYLILEATRRVRLPQPSLTVRYHAKSPDKFLKKAAEVIRLGLG 395
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 410 MPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGkWGYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQekAL 489
Cdd:cd01677  396 YPAFFNDEVVIPALLRKGVSLEDARDYGLIGCVETGAPG-RKYRWTGTGYINLAKVLEITLNNGKDPRSGKQVGPE--TG 472
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 490 SAGNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGIANL 569
Cdd:cd01677  473 DATDFKTFEELWEAFKKQLRHFIKLSVRANNISDIAHAEVAPAPFLSALVDDCIEKGKDINAGGARYNFGGIQGVGIATL 552
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 570 GNSLAAVKKLVFEQGAIGQQQLAAALADDFDGltHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHNPRyg 649
Cdd:cd01677  553 GDSLAAIKKLVFEEKKLTMEELLEALKANFAE--GYEERRRLLNNAPKYGNDDDYADNIARRVYEWYCKEVEKYQNPR-- 628
                        650       660       670       680       690       700       710       720
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 650 rgpvGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNP 729
Cdd:cd01677  629 ----GGKFYPGTYSVSANVPFGSVTGATPDGRLAGTPLSDGVSPSQGTDKKGPTAVIKSVSKLDHFNISGGTLLNQKFSP 704
                        730       740       750       760       770       780       790
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446131504 730 ATLENESDKQKLMILLRTFFEVhKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDDIIARTE 807
Cdd:cd01677  705 STLEGEEGLKKLAALIRTYFDL-GGHHIQFNVVSAETLRDAQKHPEKYRDLIVRVAGYSAYFVELSKEVQDEIIARTE 781
PflD COG1882
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ...
6-808 0e+00

Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation


Pssm-ID: 441486 [Multi-domain]  Cd Length: 789  Bit Score: 1072.47  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504   6 LDTLSDRIKAHKNALVHiVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAP 85
Cdd:COG1882    4 LAGPTERTKRLREKLLE-AKPLIDIERARLFTESYKETEGLPVIIRRAKAFSHILEHKPIYIKDDELIVGLQTDKPRKRP 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  86 IFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKaEGNMTSGDAH 165
Cdd:COG1882   83 IFPEGGIRWVEDELDALPTRPQDGFEISPEDKEIFREIAPYWKGKTHNDGVFDAYPEEIRKARKAGIIT-GLPDAYGRGH 161
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 166 LAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRRDELLT 245
Cdd:COG1882  162 IIGDYRRVLLYGLDGLIEEAKEKLAELDLTDPEDIEKIDFYKAMIIVCEAVIRLAERYAELARELAEKETDPKRKAELLE 241
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 246 MAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVElNQTLDREHAIEMLHSCWLKLLE 325
Cdd:COG1882  242 IAEICGFVPANPARTFWEAVQWVWFVYLAAIKEQNGAAMSLGRFDQYLYPYYERDLE-EGRLTEEEAQELLDCFWIKLRE 320
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 326 VNKIRSGSHSKASAGSPLYQNVTIGGQNlVDGQpmDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIR 405
Cdd:COG1882  321 VRFLRTPEYAELFAGYPTWVTLTIGGMT-PDGR--DAVNELSYLILETLRNLPLPEPNLTVRWSEKLPEGFLKKAAEVIS 397
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 406 CGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWgYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQ 485
Cdd:COG1882  398 IGTGSPQYENDDLMIPMLLNKGVTLEDARDYGIAGCVEPMVPGKQ-MQFFGAGRINLAKALEYALNNGVDEKTGKQVGPE 476
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 486 EKALSagNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVG 565
Cdd:COG1882  477 TGDPT--DFLTYDEVMEAFKKQLDYLADLYVNALNIIHYMHDKYAPEPFLSALHDDCIERGKDLNEGGARYNFGAIGIAG 554
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 566 IANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGltHEQLRQRLINgAPKYGNDDDTVDTLLARAYQTYIDELKQYHN 645
Cdd:COG1882  555 LSVVADSLSAIKKLVFDKKKVTMDELLEALAANFEG--YEELRQLLLN-APKYGNDDDYVDEIAVELVETFMDEIRKYKT 631
                        650       660       670       680       690       700       710       720
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 646 PRygrgpvGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQ 725
Cdd:COG1882  632 YR------GGTYTLSILTITSNVPYGKKTGATPDGRKAGEPLADGASPMHGRDKNGPTAVLKSVAKLPYEKATDGILLNQ 705
                        730       740       750       760       770       780       790       800
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 726 KLNPATLENESDKQKLMILLRTFFEvHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDDIIAR 805
Cdd:COG1882  706 KFSPSALGGEEGIENLVSLLRTYFD-LGGHHIQFNVVDRETLLDAQKHPEKYPDLTVRVAGYSAYFVELSKEQQDDIIAR 784

                 ...
gi 446131504 806 TEH 808
Cdd:COG1882  785 TEH 787
PFL-like pfam02901
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ...
9-671 0e+00

Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.


Pssm-ID: 427048  Cd Length: 647  Bit Score: 747.15  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504    9 LSDRIKAHK-NALVHIVKPPVCTERAQHYTEMYQQHLDK-PIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPI 86
Cdd:pfam02901   1 MWERIDVLKeNYTLYTGDPSLSWERARLLTESYKETEGVlPVDIRRAKALKKILSHLPGYIRDDELIVGLQTDKPRKRAI 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504   87 FPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKaEGNMTSGDAHL 166
Cdd:pfam02901  81 YPEGGIRWVEDELDYLNTRPQDGFEISEEDKKIFREIFPYWKGKTHNEGVFDAYTPEMKAARESGIFT-GLPDAYGRGHI 159
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  167 AVNFPLLLEKGLDGLREKVAERRSRINlTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRRDELLTM 246
Cdd:pfam02901 160 IGDYRRVLLYGLDGLIEEKEEKLAKLD-TDPEDIEKIEFYKAMIISCDAVIEYAERYARLAEELAEQETDPKRKAELLEI 238
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  247 AENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVElNQTLDREHAIEMLHSCWLKLLEV 326
Cdd:pfam02901 239 AEICGRVPARPAETFQEAIQWFWFVYLAAVKEQNGAAMSLGRLDQYLYPYYERDLE-EGRLTEEEAQELIDCFWIKLREV 317
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  327 NKIRSGSHSKASAGSPLYQNVTIGGQNLvDGQpmDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRC 406
Cdd:pfam02901 318 RFLRTPEYNKLFAGYDPFQNLTIGGQGR-DGR--DAVNKLSYLILEALDNLPLPEPNLTVRWSKKLPEEFLKKAAEVSRK 394
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  407 GFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGK-WGYRCtgmSFINFARVMLAALEGGRDATSGKVFLPQ 485
Cdd:pfam02901 395 GTGSPQYENDDVMIPALLNRGVSLEDARDYGIAGCVEPMKPGKeMQFFG---ARINLAKALEYALNGGRDELTGKQVGPK 471
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  486 EKALSagNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVG 565
Cdd:pfam02901 472 TGPVT--EFLSFEEVMEAFKKQLDYLADLYVNALNIIHYMHDKYAPEPFLSALHDDCIERGKDVGIGGARYNFSGPQGAG 549
                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  566 IANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGltHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHN 645
Cdd:pfam02901 550 LANVADSLSAIKKLVFDDKVYTLRELEDALAADFEG--EEELRQDLLNDAPKYGNDDDRVDDIAVEVVETFMDEVRKYKN 627
                         650       660
                  ....*....|....*....|....*.
gi 446131504  646 PRygrgpvGGNYYAGTSSISANVPFG 671
Cdd:pfam02901 628 YR------GGKFTPSLLTITSNVPYG 647
pflD PRK09983
putative formate acetyltransferase 2; Provisional
9-810 0e+00

putative formate acetyltransferase 2; Provisional


Pssm-ID: 182181 [Multi-domain]  Cd Length: 765  Bit Score: 584.09  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504   9 LSDRIKAHKNALVHIVKPpVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFP 88
Cdd:PRK09983   1 MTNRISRLKTALFANTRE-ISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSP 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  89 EYTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVC-PWWRGQTVQDRCYGMFTDEQKGLLATGIIKAegNMT-SGDAHL 166
Cdd:PRK09983  80 EMDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELfPYWEKRSMKDFINGQMTDEVKAATSTQIFSI--NQTdKGQGHI 157
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 167 AVNFPLLLEKGLDGLREKVAERrsrinltvLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRRDELLTM 246
Cdd:PRK09983 158 IIDYPRLLNHGLGELVAQMQQH--------CQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTI 229
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 247 AENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDveLNQTLDREHAIEMLHSCWLKLLEV 326
Cdd:PRK09983 230 AEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQAS--LTQGEDPAFLKELLESLWVKCNDI 307
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 327 NKIRSGSHSKASAGSPLYQNVTIGGqnlVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRC 406
Cdd:PRK09983 308 VLLRSTSSARYFAGFPTGYTALLGG---LTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRL 384
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 407 GFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKwGYRCTGMSFINFARVMLAAL---EGGRDATsgkvfl 483
Cdd:PRK09983 385 GTGIPQIFNDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGR-TYGLHDIAMFNLLKVMEICLhenEGNAALT------ 457
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 484 pqekalsagnfnnFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWvSGLQ 563
Cdd:PRK09983 458 -------------YEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNF-SGVQ 523
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 564 -VGIANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQ 642
Cdd:PRK09983 524 gIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEK 603
                        650       660       670       680       690       700       710       720
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 643 YHNPRygrgpvGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEGA-SPASGTDHLGPTAVIGSVGKLPTAAILGGV 721
Cdd:PRK09983 604 YQNPR------GGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQLADGGlSPMLGQDAQGPTAVLKSVSKLDNTLLSNGT 677
                        730       740       750       760       770       780       790       800
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 722 LLNQKLNPATLENESDKQKLMILLRTFFEVhKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDD 801
Cdd:PRK09983 678 LLNVKFTPATLEGEAGLRKLADFLRAFTQL-KLQHIQFNVVNADTLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDD 756

                 ....*....
gi 446131504 802 IIARTEHML 810
Cdd:PRK09983 757 IIRRTAHQL 765
choline_CutC TIGR04394
choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and ...
9-806 4.25e-144

choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and benzylsuccinate synthases, are glycine radical enzymes that appear to act as choline TMA-lyase, that is, to perform a C-N bond cleavage turning choline into trimethylamine (TMA) plus acetaldehyde. The gene symbol is cutC, for choline utilization. The activase, CutD, is a radical SAM enzyme. [Energy metabolism, Amino acids and amines]


Pssm-ID: 275187 [Multi-domain]  Cd Length: 789  Bit Score: 443.47  E-value: 4.25e-144
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504    9 LSDRIKAHKNALVHiVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFP 88
Cdd:TIGR04394   1 PTERLVRLKENYLK-QVPSITIYRARAITEIAKENPGMPKILLRAKAFRKCCETAPLVIQDDELIVGAPCGAPRAGAFSP 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504   89 EYTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVC-PWWRGQTVQDRCYGMFtdEQKGLLAtgiIKAEGNMTSGDAHlA 167
Cdd:TIGR04394  80 DIAWRWLRDELDTIGTRPQDPFYISEEDKKIMREEIfPFWEGKSLDEYCEDQY--REAGVWE---LSGESFVSDCSYH-A 153
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  168 VN-----FP----LLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRES 238
Cdd:TIGR04394 154 VNgggdsNPgydvILMKKGMLDIQAEAKEHLAELSYENPEDIDKIYFYKSVIDTTEGVMIYAKRLSEYAAELAAKEQDPK 233
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  239 RRDELLTMAE-NCDLIAHqPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVElNQTLDREHAIEMLH 317
Cdd:TIGR04394 234 RKAELEKIAEvNARVPAH-KPRTFWEAIQSVWTVESLLVVEENQTGMSIGRVDQYMYPFYKADIE-AGRMTEYEAFELAG 311
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  318 SCWLKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLVDGqpmDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFL 397
Cdd:TIGR04394 312 CMLIKMSEMMWLTSEGGSKFFAGYQPFVNMCVGGVTREGG---DATNDLTYLLMDAVRHVKVYQPSLACRIHNKSPQKYL 388
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  398 DACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWgYRCTGMSFINFARVMLAALEGGRDAT 477
Cdd:TIGR04394 389 KKIVDVVRAGMGFPACHFDDAHIKMMLAKGVSIEDARDYCLMGCVEPQKSGRL-YQWTSTAYTQWPICIELVLNHGVPLW 467
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  478 SGKVFLPQEKALSagNFNNFDEVMDAWDTQIRYYTRKSieieyVVDTMLEENVH-DI----LCSALVDDCIERAKSIKQG 552
Cdd:TIGR04394 468 YGKQVCPDTGDLS--QFDTYEKFDAAVKEQIKYITKWS-----AVATVISQRVHrDLapkpLMSLMYEGCMEKGKDVSAG 540
                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  553 GAKYDWVSGL-QVGIANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGltHEQLRQRLINgAPKYGNDDDTVDTLLA- 630
Cdd:TIGR04394 541 GAMYNFGPGVvWSGLATYADSMAAIKKLVYDDKKYTLEQLNEALKANFEG--YEQIRADCLD-APKYGNDDDYADLIAAd 617
                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  631 ---------RAYQTYIDELKQyhnprygrgpvggnyyaGTSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLG 701
Cdd:TIGR04394 618 lvnfterehRKYKTLYSHLSH-----------------GTLSISNNTPFGQLTGASANGRLAWTPLSDGISPTQGADFKG 680
                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  702 PTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQKLMILLRTFFEVHKGwHIQYNIVSRETLLEAKKHPDQYRDLV 781
Cdd:TIGR04394 681 PTAIIKSVSKMANDSMNIGMVHNFKLMSGLLDTPEGENGLITLLRTASILGNG-EMQFNYLDNETLLDAQQHPEKYRDLV 759
                         810       820
                  ....*....|....*....|....*
gi 446131504  782 VRVAGYSAFFTALSPDAQDDIIART 806
Cdd:TIGR04394 760 VRVAGYSAFFVELCKDVQDEIISRT 784
PFL1 cd01678
Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, ...
68-808 2.72e-59

Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. The PFL mechanism involves an unusual radical cleavage of pyruvate in which two cysteines and one glycine form radicals that are required for catalysis. PFL has a ten-stranded alpha/beta barrel domain that is structurally similar to those of all three ribonucleotide reductase (RNR) classes as well as benzylsuccinate synthase and B12-independent glycerol dehydratase.


Pssm-ID: 153087 [Multi-domain]  Cd Length: 738  Bit Score: 214.92  E-value: 2.72e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  68 KHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDladrpgAGFAVSEENKRVLHEvcpwWRgQTVQDRCYGMFTDEQKGL 147
Cdd:cd01678   82 KELEVIVGLQTDKPLKRAIMPFGGIRMAEQALKA------YGYELDPELKKIFTK----YR-KTHNDGVFDAYTPEIRRA 150
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 148 LATGIIkaegnmtSG--DAHlavnfplllEKG-LDGLREKVAerrsrinltvledLHGEQFL---KAIDIVLVAVSEHIE 221
Cdd:cd01678  151 RHSGII-------TGlpDAY---------GRGrIIGDYRRVA-------------LYGVDRLieeKKKDLDNLGGDEMTD 201
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 222 RFAALAREMAatetrESRR--DELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRR 299
Cdd:cd01678  202 DTIRLREEVA-----EQIKalKELKQMAASYGLDISRPATNAQEAIQWTYFGYLAAIKEQNGAAMSLGRVSTFLDIYIER 276
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 300 DVElNQTLDREHAIEMLHSCWLKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLvDGQPMdaVNPLSYAILESCGRL-R 378
Cdd:cd01678  277 DLK-AGTITEAEAQELIDQFIMKLRMVRFLRTPEYNELFSGDPTWVTESIGGMGN-DGRSL--VTKTSFRFLNTLYNLgP 352
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 379 STQPNLSVRYHAGMSNDFLDACVQV------IRcgfgmpaFNNDEIVIPEFiklgiepqdAYDYAAIGCIETAVG-GK-- 449
Cdd:cd01678  353 APEPNLTVLWSEKLPENFKRFCAKVsidtssIQ-------YENDDLMRPDW---------GGDDYGIACCVSAMRiGKqm 416
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 450 --WGYRCtgmsfiNFARVMLAALEGGRDATSGKVFLPQEKALsAGNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLE 527
Cdd:cd01678  417 qfFGARA------NLAKALLYAINGGRDEKTGDQVGPDIEPI-TSDYLDYDEVMENYDKSMDWLADTYVNALNIIHYMHD 489
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 528 ENVHDILCSALVDDCIERAksikqggakydwvsgLQVGIANLGN---SLAAVK--KLVFEQGAIGqqqlaaaLADDFDgl 602
Cdd:cd01678  490 KYAYEALQMALHDTDVRRT---------------MAFGIAGLSVaadSLSAIKyaKVKPIRDEDG-------LAVDFE-- 545
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 603 theqlrqrlINGA-PKYGNDDDTVDTLLARAYQTYIDELKQYHNPRygrgpvggNYYAGTS--SISANVPFGAQTMATPD 679
Cdd:cd01678  546 ---------IEGDfPRYGNDDDRADDIAVWVVKTFMNKLRKHKTYR--------NAEPTQSvlTITSNVVYGKKTGNTPD 608
                        650       660       670       680       690       700       710       720
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 680 GRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK--LMILLRTFFeVHKGWHI 757
Cdd:cd01678  609 GRRAGEPFAPGANPMHGRDKKGALASLASVAKLPYRDANDGISNTFSIVPNALGKTDEERIdnLVGILDGYF-TKGGHHL 687
                        730       740       750       760       770
                 ....*....|....*....|....*....|....*....|....*....|.
gi 446131504 758 QYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDDIIARTEH 808
Cdd:cd01678  688 NVNVLNRETLLDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTFH 738
Gly_radical pfam01228
Glycine radical;
687-792 1.59e-40

Glycine radical;


Pssm-ID: 426140 [Multi-domain]  Cd Length: 106  Bit Score: 144.23  E-value: 1.59e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504  687 LAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQ--KLMILLRTFFevHKGWHIQYNIVSR 764
Cdd:pfam01228   1 VAPGISPSHGADFEGPTAVLNSVGKIDYEVELDGISLNQKFLPAVLGYYDEEGyaNLNTLIDTYF--EGGHHLQFNVVDR 78
                          90       100
                  ....*....|....*....|....*...
gi 446131504  765 ETLLEAKKHPDQYRDLVVRVAGYSAFFT 792
Cdd:pfam01228  79 ETLPDAQKHPEKYPDLTVRVSGYSANFV 106
PRK11127 PRK11127
autonomous glycyl radical cofactor GrcA; Provisional
751-808 2.88e-15

autonomous glycyl radical cofactor GrcA; Provisional


Pssm-ID: 182983 [Multi-domain]  Cd Length: 127  Bit Score: 72.95  E-value: 2.88e-15
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 446131504 751 VHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDDIIARTEH 808
Cdd:PRK11127  67 VEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVIARTFT 124
RNR_PFL cd00576
Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and ...
242-467 5.57e-13

Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and pyruvate formate lyase (PFL) are believed to have diverged from a common ancestor. They have a structurally similar ten-stranded alpha-beta barrel domain that hosts the active site, and are radical enzymes. RNRs are found in all organisms and provide the only mechanism by which nucleotides are converted to deoxynucleotides. RNRs are separated into three classes based on their metallocofactor usage. Class I RNRs use a diiron-tyrosyl radical while Class II RNRs use coenzyme B12 (adenosylcobalamin, AdoCbl). Class III RNRs use an FeS cluster and S-adenosylmethionine to generate a glycyl radical. PFL, an essential enzyme in anaerobic bacteria, catalyzes the conversion of pyruvate and CoA to acteylCoA and formate in a mechanism that uses a glycyl radical.


Pssm-ID: 153083 [Multi-domain]  Cd Length: 401  Bit Score: 71.41  E-value: 5.57e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 242 ELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELnQTLDREHAIEMLHSCWL 321
Cdd:cd00576   29 DYGDSLDPGIKGVNETAKSINEAIQKTYQIIALAASNQNGGGVSFARASSILSPYGSRDYAK-GSGTETDAVEAADAFNL 107
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 322 KLLEVNKirsgshskaSAGSPLYQNVTIGGQNLvDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSN------D 395
Cdd:cd00576  108 ALKEVGQ---------GNGRTGAATGFIGGVHK-GKGDKISQEFLNLALANGGEGIPLNFPNLSVRVSSDKPGilvkavE 177
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446131504 396 FLDACVQVIRcGFGMPAFNNDEI--------------VIP-----------EFIKLGIEPQDAYDYAAIGCIETAVGGKW 450
Cdd:cd00576  178 LKQLIAEEAR-KTGSPGIFNDELcnlvslnlarimekAINgsmdvvleeleELAFLAVRALDCVIDSHDERIPTIELGGD 256
                        250
                 ....*....|....*..
gi 446131504 451 GYRCTGMSFINFARVML 467
Cdd:cd00576  257 ERRTVGLGIAGVADLLI 273
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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