NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|446111323|ref|WP_000189178|]
View 

MULTISPECIES: nitroreductase NfsA [Enterobacteriaceae]

Protein Classification

NADPH-dependent oxidoreductase( domain architecture ID 10793472)

NADPH-dependent oxidoreductase, similar to nitroreductase NfsA, which catalyzes the reduction of flavin or nitrocompounds using NAD(P)H as electron donor

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
PRK10765 PRK10765
oxygen-insensitive NADPH nitroreductase;
1-240 1.83e-175

oxygen-insensitive NADPH nitroreductase;


:

Pssm-ID: 182710  Cd Length: 240  Bit Score: 481.78  E-value: 1.83e-175
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   1 MTPTIELICGHRSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFC 80
Cdd:PRK10765   1 MTPTIELILSHRSIRHFTDEPISEAQREAIINAARAASSSSFLQCSSIIRITDKALREALVELTGGQKYVAQAAEFWVFC 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  81 ADFNRHLQICPDAQLGLAEQLLLGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP 160
Cdd:PRK10765  81 ADFNRHLQICPDAQLGLAEQLLIGAVDTAIMAQNALLAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323 161 ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPFILDYLHKQGWATR 240
Cdd:PRK10765 161 AQNPDLKPRLPASLLVHENQYQPLDKDLLAQYDEQLAEYYLTRSSNNRQDTWSDHIRRTLIKESRPFILDYLHKQGWATR 240
 
Name Accession Description Interval E-value
PRK10765 PRK10765
oxygen-insensitive NADPH nitroreductase;
1-240 1.83e-175

oxygen-insensitive NADPH nitroreductase;


Pssm-ID: 182710  Cd Length: 240  Bit Score: 481.78  E-value: 1.83e-175
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   1 MTPTIELICGHRSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFC 80
Cdd:PRK10765   1 MTPTIELILSHRSIRHFTDEPISEAQREAIINAARAASSSSFLQCSSIIRITDKALREALVELTGGQKYVAQAAEFWVFC 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  81 ADFNRHLQICPDAQLGLAEQLLLGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP 160
Cdd:PRK10765  81 ADFNRHLQICPDAQLGLAEQLLIGAVDTAIMAQNALLAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323 161 ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPFILDYLHKQGWATR 240
Cdd:PRK10765 161 AQNPDLKPRLPASLLVHENQYQPLDKDLLAQYDEQLAEYYLTRSSNNRQDTWSDHIRRTLIKESRPFILDYLHKQGWATR 240
NfsA-like cd02146
nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin ...
 4-225 1.74e-107

nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.


Pssm-ID: 380322 [Multi-domain]  Cd Length: 229  Bit Score: 309.17  E-value: 1.74e-107
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   4 TIELICGHRSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADF 83
Cdd:cd02146    1 TIETILNHRSVRKFTDEPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKLAELAGNQPYVAQAPVFLVFCADL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  84 NRHLQIC-----PDAQLGLAEQLLLGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLG 158
Cdd:cd02146   81 YRHQKIAeeaggKDVGLDYLESFLVGVVDAALAAQNALVAAESLGLGIVYIGGIRNNPEEVIELLGLPEYVFPLFGLTVG 160

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446111323 159 WPADNPDLKPRLPASILVHENSYQPL-DKGALAQYDEQLAEYYLTRGSNNRRDTWSDHI-RRTIIKESR 225
Cdd:cd02146  161 HPDPTPEVKPRLPLEAVVHEETYQDLtDLEDLAAYDETMSEYYAERGSNKRTETWSQQIaDRYLSKRDR 229
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 4-173 9.95e-37

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 126.89  E-value: 9.95e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   4 TIELICGHRSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLT--GGQKHVAQAAEFWVFCA 81
Cdd:COG0778    1 LLELLLTRRSVRKFTDKPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEALaeANQEWVADAPVLIVVCA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  82 DFNRHLQICPDAQLglaeqlllgvVDTAMMAQNALIAAESLGLGGVYIGGLrnNIEAVTKLLKLPQHVLPLFGLCLGWPA 161
Cdd:COG0778   81 DPDRSEKVPERYAL----------LDAGIAAQNLLLAARALGLGTCWIGGF--DPEKVRELLGLPEGEEPVALLALGYPA 148

                        170
                 ....*....|..
gi 446111323 162 DNPDLKPRLPAS 173
Cdd:COG0778  149 EELNPRPRKPLE 160
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 10-159 3.47e-15

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 70.88  E-value: 3.47e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   10 GHRSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQ-KHVAQAAEFWVFCADFNR--- 85
Cdd:pfam00881   3 QRRSVRKFDPEPVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELlLVEPAAALLLLLRRDANLkll 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   86 --------HLQICPDAQLGLAEQL------LLGVVDTAMMAQNALIAAESLGLGGVYIGGLrnNIEAVTKLLKLPQHVLP 151
Cdd:pfam00881  83 lqdflrgaPVLIVITASLSTYLRKaaerayREALLDAGAAAQNLLLAATSLGLGSCPIGGF--DAAAVRELLGLPDDERL 160


                  ....*...
gi 446111323  152 LFGLCLGW 159
Cdd:pfam00881 161 VGLIAVGY 168
 
Name Accession Description Interval E-value
PRK10765 PRK10765
oxygen-insensitive NADPH nitroreductase;
1-240 1.83e-175

oxygen-insensitive NADPH nitroreductase;


Pssm-ID: 182710  Cd Length: 240  Bit Score: 481.78  E-value: 1.83e-175
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   1 MTPTIELICGHRSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFC 80
Cdd:PRK10765   1 MTPTIELILSHRSIRHFTDEPISEAQREAIINAARAASSSSFLQCSSIIRITDKALREALVELTGGQKYVAQAAEFWVFC 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  81 ADFNRHLQICPDAQLGLAEQLLLGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP 160
Cdd:PRK10765  81 ADFNRHLQICPDAQLGLAEQLLIGAVDTAIMAQNALLAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323 161 ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPFILDYLHKQGWATR 240
Cdd:PRK10765 161 AQNPDLKPRLPASLLVHENQYQPLDKDLLAQYDEQLAEYYLTRSSNNRQDTWSDHIRRTLIKESRPFILDYLHKQGWATR 240
NfsA-like cd02146
nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin ...
 4-225 1.74e-107

nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.


Pssm-ID: 380322 [Multi-domain]  Cd Length: 229  Bit Score: 309.17  E-value: 1.74e-107
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   4 TIELICGHRSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADF 83
Cdd:cd02146    1 TIETILNHRSVRKFTDEPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKLAELAGNQPYVAQAPVFLVFCADL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  84 NRHLQIC-----PDAQLGLAEQLLLGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLG 158
Cdd:cd02146   81 YRHQKIAeeaggKDVGLDYLESFLVGVVDAALAAQNALVAAESLGLGIVYIGGIRNNPEEVIELLGLPEYVFPLFGLTVG 160

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446111323 159 WPADNPDLKPRLPASILVHENSYQPL-DKGALAQYDEQLAEYYLTRGSNNRRDTWSDHI-RRTIIKESR 225
Cdd:cd02146  161 HPDPTPEVKPRLPLEAVVHEETYQDLtDLEDLAAYDETMSEYYAERGSNKRTETWSQQIaDRYLSKRDR 229
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 4-173 9.95e-37

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 126.89  E-value: 9.95e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   4 TIELICGHRSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLT--GGQKHVAQAAEFWVFCA 81
Cdd:COG0778    1 LLELLLTRRSVRKFTDKPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEALaeANQEWVADAPVLIVVCA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  82 DFNRHLQICPDAQLglaeqlllgvVDTAMMAQNALIAAESLGLGGVYIGGLrnNIEAVTKLLKLPQHVLPLFGLCLGWPA 161
Cdd:COG0778   81 DPDRSEKVPERYAL----------LDAGIAAQNLLLAARALGLGTCWIGGF--DPEKVRELLGLPEGEEPVALLALGYPA 148

                        170
                 ....*....|..
gi 446111323 162 DNPDLKPRLPAS 173
Cdd:COG0778  149 EELNPRPRKPLE 160
Nitro_FMN_reductase cd02062
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 12-158 7.58e-21

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380311 [Multi-domain]  Cd Length: 139  Bit Score: 85.04  E-value: 7.58e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  12 RSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLT-GGQKHVAQAAEFWVFCADFNRHLQic 90
Cdd:cd02062    5 RSIRKFTDKPVPEEKLRKILEAARLAPSAGNLQPWRFIVVRDREKKEKLAKLAaPNQKFIAGAPVVIVVVADPDKSRP-- 82

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446111323  91 pdaqlglaeqllLGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLG 158
Cdd:cd02062   83 ------------WALEDAGAAAQNLLLAAAALGLGSCWIGGFDFREDKVRELLGIPENLRPVALIAIG 138
nitroreductase cd02139
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 5-179 3.42e-16

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380316 [Multi-domain]  Cd Length: 165  Bit Score: 73.27  E-value: 3.42e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   5 IELICGHRSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFN 84
Cdd:cd02139    2 YEAIKKRRSIRKYKPTPVEEEKLLRILEAARLAPSAKNRQPWRFIVVKDKELKEKLAEAANGQKFIAEAPVVIVACADPS 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  85 RHLQIC-PDAqlglaeqlllGVVDTAMMAQNALIAAESLGLGGVYIGGLRNniEAVTKLLKLPQHVLPLFGLCLGWPADN 163
Cdd:cd02139   82 ESGMGCgKPY----------YLVDVAIAMEHLVLAATEEGLGTCWIGAFDE--DKVKEILGIPEEYRVVALTPLGYPAEE 149

                        170
                 ....*....|....*.
gi 446111323 164 PDLKPRLPASILVHEN 179
Cdd:cd02139  150 PPPRPRKPLEEIVFYE 165
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 10-159 3.47e-15

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 70.88  E-value: 3.47e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   10 GHRSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQ-KHVAQAAEFWVFCADFNR--- 85
Cdd:pfam00881   3 QRRSVRKFDPEPVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELlLVEPAAALLLLLRRDANLkll 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   86 --------HLQICPDAQLGLAEQL------LLGVVDTAMMAQNALIAAESLGLGGVYIGGLrnNIEAVTKLLKLPQHVLP 151
Cdd:pfam00881  83 lqdflrgaPVLIVITASLSTYLRKaaerayREALLDAGAAAQNLLLAATSLGLGSCPIGGF--DAAAVRELLGLPDDERL 160


                  ....*...
gi 446111323  152 LFGLCLGW 159
Cdd:pfam00881 161 VGLIAVGY 168
MhqN-like cd02137
nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily ...
 6-173 1.54e-13

nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily of the nitroreductase family containing uncharacterized proteins; includes nitroreductases MhqN, YodC, YdgI, DrgA. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380314 [Multi-domain]  Cd Length: 147  Bit Score: 65.72  E-value: 1.54e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   6 ELICGHRSIRHFTDE-PISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADfn 84
Cdd:cd02137    2 EVIKSRRSVRNFDPDhKIPKEELKEILELATLAPSSFNLQPWRFVVVRDPELKAKLAEAAYNQPQVTTASAVILVLGD-- 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  85 rhlqicpdaqlglaeqlllgvVDTAMMAQNALIAAESLGLGGVYIGGLrnNIEAVTKLLKLPQHVLPLFGLCLGWPADNP 164
Cdd:cd02137   80 ---------------------LNAGLAAMNLMLAAKAKGYDTCPMGGF--DKEKVAELLNLPDRYVPVLLIAIGKAADKA 136


                 ....*....
gi 446111323 165 DLKPRLPAS 173
Cdd:cd02137  137 PRSGRLPVD 145
nitroreductase cd02150
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 12-169 1.60e-12

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380325 [Multi-domain]  Cd Length: 156  Bit Score: 63.39  E-value: 1.60e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  12 RSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADfnRHLQICP 91
Cdd:cd02150    5 RSIRKYTDKPVEEEDIEKLLRAAMAAPSAGNQQPWHFIVVTDREKLDKIAEAHPYGKMLKEAPLAIVVCGD--PSKEKAP 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  92 DAQlglaeqlllgVVDTAMMAQNALIAAESLGLGGVYIGG--LRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPR 169
Cdd:cd02150   83 GYW----------VQDCSAATENILLAAHALGLGAVWLGVypFEERVKAIREILNIPENIIPFCVIALGYPAEEKEPKDR 152
PnbA_NfnB-like cd02136
nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as ...
 12-176 6.70e-10

nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as a cofactor and catalyze reduction of a variety of nitroaromatic compounds, including nitrofurans, nitrobenzens, nitrophenol, nitrobenzoate and quinones by using either NADH or NADPH as a source of reducing equivalents in an obligatory two-election transfer mechanism. The enzyme is typically a homodimer. Mycobacterium smegmatis nitroreductase NfnB plays a role in resistance to benzothiazinone.


Pssm-ID: 380313 [Multi-domain]  Cd Length: 152  Bit Score: 56.06  E-value: 6.70e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  12 RSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKAlREELVTLTGGqkhvAQAAEFwvfcadfnrhlqICP 91
Cdd:cd02136    6 RSVRAFKDKPVPKETIEKILEAARRAPSGKNTQPWRVYVVTGKA-RERLKKAFFG----APVALF------------LTM 68

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  92 DAQLGLAeqlllGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDL----K 167
Cdd:cd02136   69 DKVLGPW-----SWFDLGAFLQNLMLAAHALGLGTCPQGALAGYPDVVRKELGIPDDEELVCGIALGYPDPDAPVnqfrT 143


                 ....*....
gi 446111323 168 PRLPASILV 176
Cdd:cd02136  144 PREPLEEFV 152
nitroreductase cd20608
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 6-159 1.94e-08

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380329 [Multi-domain]  Cd Length: 145  Bit Score: 51.57  E-value: 1.94e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   6 ELICGHRSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGG-QKHVAQAAEFWVFCADFN 84
Cdd:cd20608    2 EAIKTRRSVRRFSDKPVEEEKLEKILEAARLAPSWANKQCWRFIVVTDKETLSELAKKESPsNGWLKDAPVIIVVCADPK 81

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446111323  85 RHlqicpdaqlGLAEQLLLGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNieAVTKLLKLPQHVLPLFGLCLGW 159
Cdd:cd20608   82 DS---------GWLNGQNYYLVDAAIAMQNLMLAATDLGLGTCWIGAFDEK--KVKEILGIPENIRVVALTPLGY 145
nitroreductase cd03370
uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus ...
 12-149 1.60e-07

uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus thermophilus NADH oxidase and other, uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380327 [Multi-domain]  Cd Length: 191  Bit Score: 50.01  E-value: 1.60e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  12 RSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNR------ 85
Cdd:cd03370    9 RSIRKYTQEPVPDEDLREILRLAGLAPSAWNIQPWRFVVVRDAELKEQLQAAAYGQAQVTSAPAVIVIYSDMEDalanle 88

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  86 ---HLQICPDAQLGLAEQLL--LGVVDTAMMAQNA-----------LIAAESLGLGGVYIGGLrnNIEAVTKLLKLPQHV 149
Cdd:cd03370   89 etiHPGLSEERRQREAAGLRgaFGKMSVEQRGQWGlaqanialgflLLAAQSLGYDTSPMLGF--DPEKVKALLGLPEHV 166
BluB cd02145
5,6-dimethylbenzimidazole synthase; BluB catalyzes the O2-dependent conversion of FMNH2 to 5, ...
 6-177 3.08e-07

5,6-dimethylbenzimidazole synthase; BluB catalyzes the O2-dependent conversion of FMNH2 to 5,6-dimethylbenzimidazole (DMB), a component of vitamin B12; is is a subfamily of the nitroreductase family; nitroreductases typically reduce their substrates by using NAD(P)H as electron donor and often use FMN as a cofactor.


Pssm-ID: 380321  Cd Length: 196  Bit Score: 49.28  E-value: 3.08e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   6 ELICGHRSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTL------------TGGQKhvAQA 73
Cdd:cd02145    2 RVIRWRRDVRHFRPDPVPEEVLERLLQAAHLAPSVGLMQPWRFVRVRSAATRKAVHELfqranaeaaemyTGERA--AQY 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  74 AEFwvfcadfnrHLQICPDAQLGLA--------EQLLLGVV--------DTAMMAQNALIA--AESLGLGGVYIgglrNN 135
Cdd:cd02145   80 RTL---------KLEGIEEAPLQLAvfcdraraGGHGLGRTtmpemdlySSVCAVQNLWLAarAEGLGVGWVSI----LD 146

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 446111323 136 IEAVTKLLKLPQHVLPLFGLCLGWPA---DNPDL-----KPRLPASILVH 177
Cdd:cd02145  147 PDEVKRLLGIPEHWEPVAYLCIGYPEffyDEPELeqagwEQRRPLEWVVF 196
nitroreductase cd02151
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 6-168 6.35e-07

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers..


Pssm-ID: 380326 [Multi-domain]  Cd Length: 157  Bit Score: 47.53  E-value: 6.35e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   6 ELICGHRSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLT-GGQKHVAQAAEFWVFCADfn 84
Cdd:cd02151    1 ELLKKRRSIRKYTDEPIEEEKLEEILEAALLAPSSRNSRPVEFIVVDDKETLKKLSECKpHGSAFLKGAPAAIVVLAD-- 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  85 rhlqicpdaqlglAEQLLLGVVDTAMMAQNALIAAESLGLGGVYIgGLRNN--------IEAVTKLLKLPQHVLPLFGLC 156
Cdd:cd02151   79 -------------TEKSDTWIEDASIAATYIQLAAESLGLGSCWI-QIRNRetqdgktaEEYVRELLGIPENYRVLCIIA 144

                        170
                 ....*....|..
gi 446111323 157 LGWPADNPDLKP 168
Cdd:cd02151  145 LGYPDEEKPPHE 156
McbC_SagB-like_oxidoreductase cd02142
oxidase similar to the microcin B17 processing protein McbC; This family is the oxidase domain ...
 48-155 3.12e-05

oxidase similar to the microcin B17 processing protein McbC; This family is the oxidase domain of NRPS (non-ribosomal peptide synthetase) and other systems that modify polypeptides by cyclizing a thioester to form a ring. These include EpoB, part of the epothilone biosynthesis pathway; TubD, part of the tubulysin biosynthesis pathway, MtsD, part of the myxothiozol biosynthesis pathway; IndC, part of the indigoidine biosynthesis pathway and TfxB, part of the trifitoxin processing pathway. All are FMN-dependent and oxidize the product of the cyclization of thioesters in short polypeptides.


Pssm-ID: 380318  Cd Length: 200  Bit Score: 43.56  E-value: 3.12e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  48 IIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAqlGLAEQLLlgvvDTAMMAQNALIAAESLGLGGV 127
Cdd:cd02142   96 LVLIREGDFRLDLAHAAGNQAAFGSAAFSLIIVARFERIAWKYGER--AYRYILL----EAGHLAQNLYLAATALGLGLC 169

                         90       100       110
                 ....*....|....*....|....*....|.
gi 446111323 128 YIGGLrnNIEAVTKLLKLP---QHVLPLFGL 155
Cdd:cd02142  170 AIGAF--DDDALRELLGLDeveEVVLYAFVV 198
nitroreductase cd20609
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 5-158 8.28e-05

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380330 [Multi-domain]  Cd Length: 145  Bit Score: 41.61  E-value: 8.28e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323   5 IELICGHRSIRHFTDEPISEAQRGAIINSARATSSSSFLQCSSIIRITDKALREELVTLTggqKHVAQAAEFWVFCAD-- 82
Cdd:cd20609    3 LELAKKRYSVRKFSDKPVEKEKLDKILEAGRLAPTAVNYQPQRILVVRSEEALEKLAKAT---PRFFGAPLVIVVCYDkd 79

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446111323  83 --FNRHLqicpdaqlglaEQLLLGVVDTAMMAQNALIAAESLGLGGVYIGGLrnNIEAVTKLLKLPQHVLPLFGLCLG 158
Cdd:cd20609   80 esWKRPY-----------DGKDSGDIDAAIVATHMMLAATELGLGTCWVGNF--DPEKVREAFNLPENLEPVAILPLG 144
FbiB_C-like cd20607
nitroreductase family domain similar to the C-terminal domain of F420:gamma-glutamyl ligase ...
 91-173 2.19e-04

nitroreductase family domain similar to the C-terminal domain of F420:gamma-glutamyl ligase FbiB; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Mycobacterium tuberculosis FbiB, is a two-domain protein and produces F420 with predominantly 5 to 7 L-glutamate residues in the poly-gamma-glutamate tail, its C-terminal domain is homologous to FMN-dependent nitroreductases.


Pssm-ID: 380328 [Multi-domain]  Cd Length: 155  Bit Score: 40.53  E-value: 2.19e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446111323  91 PDAQLGLAEQLLLGVvdtAMMA--QNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKP 168
Cdd:cd20607   73 PDARRTDAEHTMFTV---AVGAavQALLVALAVRGLGSCWIGSTIFAPDVVRDELDLPDDWEPLGAIAIGYPLEPPPPRP 149


                 ....*
gi 446111323 169 RLPAS 173
Cdd:cd20607  150 PAPTD 154
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH