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Conserved domains on  [gi|446103543|ref|WP_000181398|]
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MULTISPECIES: tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL [Staphylococcus]

Protein Classification

tRNA (cytidine(34)-2'-O)-methyltransferase( domain architecture ID 10000715)

tRNA (cytidine(34)-2'-O)-methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine to the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA)

EC:  2.1.1.207
Gene Ontology:  GO:0003723|GO:0008173|GO:1904047|GO:0141098|GO:0001510
SCOP:  4002340

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 3-155 1.39e-99

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


:

Pssm-ID: 439989  Cd Length: 153  Bit Score: 282.69  E-value: 1.39e-99
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543   3 NHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKMLKRAGLDYWEFVNITYHDSIEAFFE-VTNGEYYLLTKF 81
Cdd:COG0219    2 LHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEaLPGGRLFLFTTK 81

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446103543  82 GKKTYSDFDFSNHDkdfYFIFGKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGL 155
Cdd:COG0219   82 GTKPYTDVDYQPGD---VLLFGRETAGLPEEVLAAPADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGL 152
 
Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 3-155 1.39e-99

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 282.69  E-value: 1.39e-99
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543   3 NHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKMLKRAGLDYWEFVNITYHDSIEAFFE-VTNGEYYLLTKF 81
Cdd:COG0219    2 LHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEaLPGGRLFLFTTK 81

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446103543  82 GKKTYSDFDFSNHDkdfYFIFGKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGL 155
Cdd:COG0219   82 GTKPYTDVDYQPGD---VLLFGRETAGLPEEVLAAPADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGL 152
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 4-150 3.62e-90

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 258.54  E-value: 3.62e-90
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543   4 HIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKMLKRAGLDYWEFVNITYHDSIEAFFE-VTNGEYYLLTKFG 82
Cdd:cd18094    1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEaLPPGRLFLFTTKG 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446103543  83 KKTYSDFDFSNHDkdfYFIFGKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQ 150
Cdd:cd18094   81 TKPYTDFDFKPGD---YLVFGRESTGLPEEILDAFPDRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 4-155 7.54e-73

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 215.36  E-value: 7.54e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543    4 HIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKMLKRAGLDYWEFVNITYHDSIEAFFEVTNGE-YYLLTKFG 82
Cdd:TIGR00185   3 NIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEKPQkLFALTKKG 82

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446103543   83 KKTYSDFDFSNHDkdfYFIFGKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGL 155
Cdd:TIGR00185  83 TPAHSAVTYQDGD---YLMFGPETRGLPQSILDNLMEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGYPGL 152
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 4-154 1.09e-47

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 151.69  E-value: 1.09e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543   4 HIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKMLKRAGLDYWEFVNITYHDSIEAFFEVTNGE-YYLLTKFG 82
Cdd:PRK10358   3 NIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQrLFALTTKG 82

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446103543  83 KKTYSDFDFSNHDkdfYFIFGKETTGLPDWVKEKY-QDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPG 154
Cdd:PRK10358  83 TPAHSAVSYQDGD---YLMFGPETRGLPASILDALpAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPG 152
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 4-144 4.73e-41

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 134.20  E-value: 4.73e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543    4 HIVLYQPEIPANTGNIARTCAGTNTH-LHLIKPLGFRTDDKMLKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLL--TK 80
Cdd:pfam00588   3 VVVLDNIQDPGNLGAIIRTAAAFGADgLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAGYAVvaTS 82

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446103543   81 FGKKTYSDFDFSNHDkdfYFIFGKETTGLPDWVKEKYQDTaLRIPMSEHIRSLNLSNTAALLIY 144
Cdd:pfam00588  83 LGGKSLYEIDFPKKV---ALVFGNEGTGLSNEVLELCDRL-VRIPMSGDVESLNVAVAAAIALY 142
 
Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 3-155 1.39e-99

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 282.69  E-value: 1.39e-99
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543   3 NHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKMLKRAGLDYWEFVNITYHDSIEAFFE-VTNGEYYLLTKF 81
Cdd:COG0219    2 LHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEaLPGGRLFLFTTK 81

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446103543  82 GKKTYSDFDFSNHDkdfYFIFGKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGL 155
Cdd:COG0219   82 GTKPYTDVDYQPGD---VLLFGRETAGLPEEVLAAPADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGL 152
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 4-150 3.62e-90

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 258.54  E-value: 3.62e-90
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543   4 HIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKMLKRAGLDYWEFVNITYHDSIEAFFE-VTNGEYYLLTKFG 82
Cdd:cd18094    1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEaLPPGRLFLFTTKG 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446103543  83 KKTYSDFDFSNHDkdfYFIFGKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQ 150
Cdd:cd18094   81 TKPYTDFDFKPGD---YLVFGRESTGLPEEILDAFPDRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 4-155 7.54e-73

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 215.36  E-value: 7.54e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543    4 HIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKMLKRAGLDYWEFVNITYHDSIEAFFEVTNGE-YYLLTKFG 82
Cdd:TIGR00185   3 NIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEKPQkLFALTKKG 82

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446103543   83 KKTYSDFDFSNHDkdfYFIFGKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGL 155
Cdd:TIGR00185  83 TPAHSAVTYQDGD---YLMFGPETRGLPQSILDNLMEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGYPGL 152
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 4-154 1.09e-47

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 151.69  E-value: 1.09e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543   4 HIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKMLKRAGLDYWEFVNITYHDSIEAFFEVTNGE-YYLLTKFG 82
Cdd:PRK10358   3 NIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQrLFALTTKG 82

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446103543  83 KKTYSDFDFSNHDkdfYFIFGKETTGLPDWVKEKY-QDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPG 154
Cdd:PRK10358  83 TPAHSAVSYQDGD---YLMFGPETRGLPASILDALpAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPG 152
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 4-144 4.73e-41

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 134.20  E-value: 4.73e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543    4 HIVLYQPEIPANTGNIARTCAGTNTH-LHLIKPLGFRTDDKMLKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLL--TK 80
Cdd:pfam00588   3 VVVLDNIQDPGNLGAIIRTAAAFGADgLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAGYAVvaTS 82

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446103543   81 FGKKTYSDFDFSNHDkdfYFIFGKETTGLPDWVKEKYQDTaLRIPMSEHIRSLNLSNTAALLIY 144
Cdd:pfam00588  83 LGGKSLYEIDFPKKV---ALVFGNEGTGLSNEVLELCDRL-VRIPMSGDVESLNVAVAAAIALY 142
SpoU-like_family cd18082
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 4-146 1.49e-31

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349955  Cd Length: 145  Bit Score: 110.01  E-value: 1.49e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543   4 HIVLYQPEIPANTGNIARTCAGTN-THLHLIKPLGFRTDDKMlKRAGLDYWEFVNITYHDSIE---AFFEVTNGEYYLL- 78
Cdd:cd18082    1 HLVLDNIRSPGNIGSIFRTADAFGvEKIYLVGPTADIFDPKA-KRTSRGTHFSVPYERVDNFEeylKSLKEEGYPLIGLe 79

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446103543  79 TKFGKKTYSDFDFSNhDKDFYFIFGKETTGLPDWVkEKYQDTALRIPMSEHIRSLNLSNTAALLIYEA 146
Cdd:cd18082   80 LTDGSIPLSEFKFKK-PKKIALIFGNERSGLSPEL-LALCDYTVHIPMSGKNSSLNVSVATGIALYEF 145
SpoU-like_RNA-MTase cd18104
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 5-150 6.85e-14

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349977  Cd Length: 146  Bit Score: 64.52  E-value: 6.85e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543   5 IVLYQPEIPANTGNIARTCAGTNTHLHLIkplgfrTDDKM------LKRAGLDYWEFVNITYHDSIEAF-FEVTNGEYYL 77
Cdd:cd18104    3 LVLEGLEKPGNLGAILRSADAAGADAVIL------CDPKTdlfnpnVIRASLGAVFTLPVAEASSEETLaWLKENKIRIL 76

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446103543  78 LTKF-GKKTYSDFDFSNHdkdFYFIFGKETTGL-PDWVKEKyqDTALRIPMSEHIRSLNLSNTAALLIYEALRQQ 150
Cdd:cd18104   77 ATTPkAEKLYYEADLRGP---IAIVVGSESEGLsEFWLKAA--DERVRIPMAGQADSLNVSVAAAILLYEALRQR 146
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 82-150 1.97e-11

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 59.82  E-value: 1.97e-11
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446103543  82 GKKTYSDFDFSnhdKDFYFIFGKETTGLPDWVKEKYqDTALRIPMSEHIRSLNLSNTAALLIYEALRQQ 150
Cdd:COG0566  198 GEASLYEVDLP---GPVALVLGNEGKGLSRLVLEAC-DELVRIPMAGGVESLNVAVAAAILLYEAARQR 262
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 11-149 1.94e-10

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 55.94  E-value: 1.94e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543  11 EIPANTGNIA---RTC--AGTNtHLHLIKPL-GFRTDDKMLKRAglDYWefVNITYHDSIEAFFEVTNGEYYLL--TKFG 82
Cdd:cd18092   22 ENVHDPHNASavlRTCdaFGVQ-DVHVIEPKnKFKKNRGVAMGA--DKW--LDVHRHDSTEECLAALKEQGYRIvaTSLS 96

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446103543  83 KKTYS--DFDFsnhDKDFYFIFGKETTGLPDWVKEkYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQ 149
Cdd:cd18092   97 PDAVPldELDY---TRPTALVFGNEKDGVSEEALA-AADERVYIPMYGFVESLNVSVAAAIILYELQRQ 161
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 13-146 2.81e-09

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 52.43  E-value: 2.81e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543  13 PANTGNIARTCAGtnthlhlikpLGFRT-----------DDKMLkRAGLDYWEFVNITYHDSIEAFFEvtngeyyLLTKF 81
Cdd:cd18095   11 PGNLGTIIRTAAA----------FGVDGvilskgtvdpyNPKVV-RASMGALFRLPIIRVDDLEELLE-------ELKEA 72

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446103543  82 GKKTY-------SDFDFSNHDKDFYFIFGKETTGLPDWVKEkYQDTALRIPMSEHIRSLNLSNTAALLIYEA 146
Cdd:cd18095   73 GYKVVaadldggSSLYDLDLPKPVALVLGNEGNGLSDELLE-LCDKRVTIPMSGGVESLNVAVAAAILLYEL 143
SpoU-like_RlmB cd18103
SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the ...
 82-146 4.78e-08

SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349976  Cd Length: 143  Bit Score: 48.92  E-value: 4.78e-08
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446103543  82 GKKTYSDFDFsnhDKDFYFIFGKETTGLPDWVKEKyQDTALRIPMSEHIRSLNLSNTAALLIYEA 146
Cdd:cd18103   83 AEKSLYDVDL---TGPLALVLGSEGKGLRRLVLKN-CDFLVKIPMAGKVDSLNVSVAAGIILYEI 143
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 4-145 8.31e-08

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 48.65  E-value: 8.31e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543   4 HIVLYQPEIPANTGNIARTCAgtN---THLHLIKPLGFRTDDKMLKRA--GLDYWEfvNITYHDSIEaffEVTNGEYYL- 77
Cdd:cd18093    2 RVVLVEPQHPGNIGAVARAMK--NfglSDLRLVNPRCDDPEEAARALAsgAEDVLE--NAKVYDTLE---EALADCDLVv 74

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543  78 -----LTKFGKKTYSDFDF------SNHDKDFYFIFGKETTGLPDwvKE-KYQDTALRIPMSEHIRSLNLSNTAALLIYE 145
Cdd:cd18093   75 attarPRGLRRPLLTPRELaellkeHAAGGKVALVFGREDSGLTN--EElALCDLLVTIPTNPEYPSLNLAQAVAIVLYE 152
SpoU-like_AviRb cd18107
SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes ...
 5-146 2.83e-05

SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA. AviRb is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349980  Cd Length: 148  Bit Score: 41.61  E-value: 2.83e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543   5 IVLYQPEIPANTGNIARTCAGTNTHlHLIKpLGFRTD--DKMLKRAGLDYWEFVNITYHDS---IEAFFEVTNGEYYLLT 79
Cdd:cd18107    3 VLFDRPTSPGNLGTIIRSADALGAH-GLVV-TGHAADvyDPKTIRASTGSLFSLPIVRVPSnreLEDWVEARKARGTPLQ 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446103543  80 KFGKKTYSDFDFSNHD--KDFYFIFGKETTGLPDWVKEKyQDTALRIPMSEHIRSLNLSNTAALLIYEA 146
Cdd:cd18107   81 LVGTDEHGDHQIADHDftRPTLLLIGNETAGLSAAWREL-CDETVSIPMAGSASSLNAACAASIFLYEA 148
PRK11081 PRK11081
tRNA guanosine-2'-O-methyltransferase; Provisional
 13-155 2.90e-05

tRNA guanosine-2'-O-methyltransferase; Provisional


Pssm-ID: 236837 [Multi-domain]  Cd Length: 229  Bit Score: 42.29  E-value: 2.90e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543  13 PANTGNIARTCAGTNTH-LHLIKPlgfrtDDKMLKR----AGLDYWefVNITYHDSIEAFFEVTNGE--YYLLTKFGKKT 85
Cdd:PRK11081  30 PHNVSAIIRTADAVGVHeVHAVWP-----GSRMRTMgstaAGSNSW--VQVKTHRTIGDAVAHLKGQgmQILATHLSDTA 102

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543  86 YsDFDFSNHDKDFYFIFGKETTGLPDWVKeKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGL 155
Cdd:PRK11081 103 V-DFREIDYTRPTCILMGQEKTGISQEAL-ALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGM 170
rRNA_methyl_3 TIGR00186
rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases ...
 101-149 3.62e-05

rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 129290 [Multi-domain]  Cd Length: 237  Bit Score: 42.33  E-value: 3.62e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 446103543  101 IFGKETTGLPDWVKEKyQDTALRIPMSEHIRSLNLSNTAALLIYEALRQ 149
Cdd:TIGR00186 189 VVGNEGEGVSRLIKEN-CDFLIKIPMAGKVDSLNVSVAAGILLFEIKRQ 236
SpoU-like cd18097
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 4-146 5.93e-05

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349970  Cd Length: 144  Bit Score: 40.63  E-value: 5.93e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543   4 HIVLYQPEIPANTGNIARTCAGTN-THLHL----IKPlgfrTDDKMLKRA-GLDywEFVNITYH-DSIEAFFEVTNGEYY 76
Cdd:cd18097    2 VVVLDNLRSAHNVGSIFRTADAAGvEKIYLcgytPTP----PHPKVAKTAlGAE--ESVPWEHFeDTLEAVRALKARGYT 75

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446103543  77 LL---TKFGKKTYSDFDFSnhdKDFYFIFGKETTGL-PDWVKEkyQDTALRIPMSEHIRSLNLSNTAALLIYEA 146
Cdd:cd18097   76 IIaleTTENSVPLHEARLP---RPLALVLGNEVRGVsPEVLAL--CDAVVEIPMFGRKNSLNVAVAAAVALYEL 144
SpoU-like_TRM3-like cd18091
SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O) ...
 13-145 6.80e-05

SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O)-methyltransferase TRM3 catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in various tRNAs. TRM3 is similar to C-terminal domain of TAR (HIV-1) RNA binding protein 1 (TARBP1), a protein binding to TAR, which functions as a RNA regulatory signal by forming a stable stem-loop structure to which transactivator protein Tat binds. The role of TARBP1 is believed to be to disengage RNA polymerase II from TAR during transcriptional elongation. TRM3 and the C-terminal methyltransferase domain of TARBP1 are members of the SPOUT methyltransferase superfamily.


Pssm-ID: 349964  Cd Length: 145  Bit Score: 40.59  E-value: 6.80e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543  13 PANTGNIARTCA--GTNThlhLIKPLGFRTDDKMLKRAGLDYWEFVNITYH--DSIEAFFEVTNGEYYLLTKFGKKTYS- 87
Cdd:cd18091   11 PPNLGGLCRTCEifGVSK---LVLPNLAVVEDKQFKALSVSAEKWLPIEEVkpEDLLPYLKEKKQEGYTIVGLEQTANSv 87

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 446103543  88 ---DFDFsnhDKDFYFIFGKETTGLPDWVKeKYQDTALRIPMSEHIRSLNLSNTAALLIYE 145
Cdd:cd18091   88 slhDFQF---PKKTVLVLGNEKEGIPAELL-SLLDVCVEIPQLGVIRSLNVHVSGAIAIYE 144
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 5-150 8.11e-05

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 41.26  E-value: 8.11e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543   5 IVLYQPEIPANTGNIARTCAgtN---THLHLIKPLGFRtDDKMLKRA--GLDYWEfvNITYHDSIEAFFEvtngeyyllt 79
Cdd:COG0565    6 VVLVEPQHPGNIGAAARAMK--NfglSDLRLVNPRGWP-DEEARAMAsgADDVLE--NARVVDTLEEALA---------- 70

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543  80 kfgkktysDFDF--------SNHDKDFY-------------------FIFGKETTGLPDwvKEKYQ-DTALRIPMSEHIR 131
Cdd:COG0565   71 --------DCDLvvgttarpRELRKPVLtpreaaeellaraaggkvaLVFGRERSGLTN--EELALcDALVTIPTNPEYP 140

                        170
                 ....*....|....*....
gi 446103543 132 SLNLSNTAALLIYEaLRQQ 150
Cdd:COG0565  141 SLNLAQAVQVVAYE-LRKA 158
rRNA_methyl_1 TIGR00050
RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of ...
 5-151 1.17e-03

RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases, and is now characterized, in E. coli, as a tRNA:Cm32/Um32 methyltransferase. It may be named TrMet(Xm32), or TrmJ, according to the nomenclature style chosen [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272876 [Multi-domain]  Cd Length: 233  Bit Score: 37.76  E-value: 1.17e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543    5 IVLYQPEIPANTGNIARtcAGTN---THLHLIKPLGFRTDDkmlkragldywefvniTYHDSIEAFFEVTNGeyylltkf 81
Cdd:TIGR00050   6 IVLVEPSHPGNIGSIAR--AMKNmglTELRLVNPRVKLEEE----------------AYALAAGARDILDNA-------- 59

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446103543   82 gkKTYSDFDFSNHDKDF--------------------------------YFIFGKETTGLPDwvkEKYQ--DTALRIPMS 127
Cdd:TIGR00050  60 --KVVDTLDEALDDCDFvvgtsarsrnlqrpmltprelapklvaekgkvALVFGREDSGLTN---EELQkcHVLVSIPTS 134

                         170       180
                  ....*....|....*....|....*...
gi 446103543  128 EHIRSLNLSNTAALLIYE----ALRQQD 151
Cdd:TIGR00050 135 EEYPSLNLSHAVAVILYElrmaWLAQQE 162
SpoU-like_MRM1 cd18105
SAM-dependent rRNA methylase related to MRM1; MRM1 catalyzes the methylation of 2'-O-ribose ...
 101-145 9.48e-03

SAM-dependent rRNA methylase related to MRM1; MRM1 catalyzes the methylation of 2'-O-ribose residues G1145 to GmG residue of the mitochondrial 16S rRNA. MRM1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349978  Cd Length: 158  Bit Score: 34.80  E-value: 9.48e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 446103543 101 IFGKETTGLPDWVKEKyQDTALRIPMSE-----HIRSLNLSNTAALLIYE 145
Cdd:cd18105  109 VLGNEGRGLRTNIKNL-CDYLVSIPGGRsaenpGVDSLNVSVAAGILLHS 157
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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