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Conserved domains on  [gi|1437472201|emb|SUL29754|]
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regulatory protein BlaR1 [Staphylococcus aureus]

Protein Classification

M56 family metallopeptidase( domain architecture ID 11467994)

M56 family metallopeptidase is an integral membrane metallopeptidase, containing a zinc-binding HEXXH motif; it allows bacteria to respond to presence of beta-lactam antibiotics by expression of beta-lactamases and penicillin-binding proteins; similar to transmembrane proteins BlaR1 and MecR1

EC:  3.4.-.-
Gene Ontology:  GO:0008233|GO:0046872|GO:0016020
MEROPS:  M56
PubMed:  2404938|11245199|11239156|24596965|23733187

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MecR1 COG4219
Signal transducer regulating beta-lactamase production, contains metallopeptidase domain ...
 110-324 1.12e-31

Signal transducer regulating beta-lactamase production, contains metallopeptidase domain [Signal transduction mechanisms];


:

Pssm-ID: 443363 [Multi-domain]  Cd Length: 337  Bit Score: 121.31  E-value: 1.12e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201 110 WIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKI-DTILFNHQYKKNIVIRKAETIQSPITFWYGKYIILIPSSyFK 188
Cdd:COG4219     1 WLAGVLLLLLRLLISLLRLRRLLRRARPVTDEELLELlERLARRLGIRRPVRLLESDRITSPFSFGLLRPVILLPAG-LE 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201 189 SVIDKRLKYIILHEYAHAKNRDTLHLIIFNIFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNInkNEFKTYAESIMDS 268
Cdd:COG4219    80 ELSEEELEAILAHELAHIRRRDLLDNLLAELLLALFWFNPLVWLARRRLRLDRELACDAAVLKAG--GDRKAYAETLLKL 157

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1437472201 269 VLNvpfFNKNILSHSFNGKKSLLKRRLINIKEANlKKQSKLILIFICIFTFLLMVI 324
Cdd:COG4219   158 AER---RSQPALALAFGGSKSTLKKRIKMLLKSK-SKRRSRLKLLLALLLALLLAL 209
 
Name Accession Description Interval E-value
MecR1 COG4219
Signal transducer regulating beta-lactamase production, contains metallopeptidase domain ...
 110-324 1.12e-31

Signal transducer regulating beta-lactamase production, contains metallopeptidase domain [Signal transduction mechanisms];


Pssm-ID: 443363 [Multi-domain]  Cd Length: 337  Bit Score: 121.31  E-value: 1.12e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201 110 WIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKI-DTILFNHQYKKNIVIRKAETIQSPITFWYGKYIILIPSSyFK 188
Cdd:COG4219     1 WLAGVLLLLLRLLISLLRLRRLLRRARPVTDEELLELlERLARRLGIRRPVRLLESDRITSPFSFGLLRPVILLPAG-LE 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201 189 SVIDKRLKYIILHEYAHAKNRDTLHLIIFNIFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNInkNEFKTYAESIMDS 268
Cdd:COG4219    80 ELSEEELEAILAHELAHIRRRDLLDNLLAELLLALFWFNPLVWLARRRLRLDRELACDAAVLKAG--GDRKAYAETLLKL 157

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1437472201 269 VLNvpfFNKNILSHSFNGKKSLLKRRLINIKEANlKKQSKLILIFICIFTFLLMVI 324
Cdd:COG4219   158 AER---RSQPALALAFGGSKSTLKKRIKMLLKSK-SKRRSRLKLLLALLLALLLAL 209
Peptidase_M56 pfam05569
BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which ...
 27-298 1.31e-23

BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which mediate staphylococcal resistance to beta-lactam antibiotics) is regulated by a signal-transducing integral membrane protein and a transcriptional repressor. The signal transducer is a fusion protein with penicillin-binding and zinc metalloprotease domains. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. homologs to this peptidase domain, which corresponds to Merops family M56, are also found in a number of other bacterial genome sequences.


Pssm-ID: 428523  Cd Length: 296  Bit Score: 98.31  E-value: 1.31e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201  27 KRYFNYMLNYKVWYLTLLAGLIPFIPI-KFSLFKFNNVN---NQAPTVESKSHDLNHNINTTkpiqefATDIHKFNWDSI 102
Cdd:pfam05569  25 RRYLGAIWAYALWLIVPLALLAPFIPEsALSNFTTSAIFmarSKAPINSTAPKVIDDQASPL------ATPFPVVAWSTI 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201 103 DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLylNENEKNKIDTILF----NHQYKKNIVIRKAETIQSPITFWYGKY 178
Cdd:pfam05569  99 LKILLLLWIVGALILLLYMIAAYLVFRQRRVRRL--GSLRAHELDDILKeakeDMGIKRPITISLSSNIDSPAVLGLWKP 176

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201 179 IILIPSSYFKSVIDKRLKYIILHEYAHAKNRDtlhlIIFNIFS----IIMSYNPLVHIVKRKIIHDNEVEADRFVLNNIN 254
Cdd:pfam05569 177 RIVLPADFDTRLSGEEIDYILAHELSHLKRGD----LIINLLVavlqCLHWFNPLVHLAFRKIRIDQELACDAAVLARLH 252

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....
gi 1437472201 255 KNEFKTYAESIMDSVLNVPFFNKNILSHSFNGKKSLLKRRLINI 298
Cdd:pfam05569 253 PHERKEYGRTLLKLLAGPSNHIVPVACVWLAGAKSALKERIMML 296
M56_BlaR1_MecR1_like cd07341
Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; ...
 109-302 2.50e-22

Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; This family contains peptidase M56, which includes zinc metalloprotease domain in MecR1 as well as BlaR1. MecR1 is a transmembrane beta-lactam sensor/signal transducer protein that regulates the expression of an altered penicillin-binding protein PBP2a, which resists inactivation by beta-lactam antibiotics, in methicillin-resistant Staphylococcus aureus (MRSA). BlaR1 regulates the inducible expression of a class A beta-lactamase that hydrolytically destroys certain ?-lactam antibiotics in MRSA. Both, MecR1 and BlaR1, are transmembrane proteins that consist of four transmembrane helices, a cytoplasmic zinc protease domain, and the soluble C-terminal extracellular sensor domain, and are highly similar in sequence and function. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. All members contain the zinc metalloprotease motif (HEXXH). Homologs of this peptidase domain are also found in a number of other bacterial genome sequences, most of which are as yet uncharacterized.


Pssm-ID: 320700 [Multi-domain]  Cd Length: 187  Bit Score: 92.39  E-value: 2.50e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201 109 IWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILfnhQYKKNIVIRKAETIQSPITFWYGKYIILIPSSyFK 188
Cdd:cd07341     1 IWLAGALLLLLRLLRGLLRLRRLRRRAEPVPDSLLLELARRL---GLRRSVRLSVSALVASPMVVGLFRPVILLPEG-LL 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201 189 SVIDKRLKYIILHEYAHAKNRDTLHLIIFNIFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNniNKNEFKTYAESIMDs 268
Cdd:cd07341    77 EGSPEELRAILLHELAHIRRRDLLVNLLQRLLEALFWFNPLVWLLSRRLRLERELACDEAVLA--ALGDKEDYAEALLR- 153

                         170       180       190
                  ....*....|....*....|....*....|....
gi 1437472201 269 VLNVPFFNKNILSHSFNGKKSLLKRRLINIKEAN 302
Cdd:cd07341   154 LAERRSQPPPALALALAGSKSLLKRRIKRILKKK 187
 
Name Accession Description Interval E-value
MecR1 COG4219
Signal transducer regulating beta-lactamase production, contains metallopeptidase domain ...
 110-324 1.12e-31

Signal transducer regulating beta-lactamase production, contains metallopeptidase domain [Signal transduction mechanisms];


Pssm-ID: 443363 [Multi-domain]  Cd Length: 337  Bit Score: 121.31  E-value: 1.12e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201 110 WIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKI-DTILFNHQYKKNIVIRKAETIQSPITFWYGKYIILIPSSyFK 188
Cdd:COG4219     1 WLAGVLLLLLRLLISLLRLRRLLRRARPVTDEELLELlERLARRLGIRRPVRLLESDRITSPFSFGLLRPVILLPAG-LE 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201 189 SVIDKRLKYIILHEYAHAKNRDTLHLIIFNIFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNInkNEFKTYAESIMDS 268
Cdd:COG4219    80 ELSEEELEAILAHELAHIRRRDLLDNLLAELLLALFWFNPLVWLARRRLRLDRELACDAAVLKAG--GDRKAYAETLLKL 157

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1437472201 269 VLNvpfFNKNILSHSFNGKKSLLKRRLINIKEANlKKQSKLILIFICIFTFLLMVI 324
Cdd:COG4219   158 AER---RSQPALALAFGGSKSTLKKRIKMLLKSK-SKRRSRLKLLLALLLALLLAL 209
Peptidase_M56 pfam05569
BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which ...
 27-298 1.31e-23

BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which mediate staphylococcal resistance to beta-lactam antibiotics) is regulated by a signal-transducing integral membrane protein and a transcriptional repressor. The signal transducer is a fusion protein with penicillin-binding and zinc metalloprotease domains. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. homologs to this peptidase domain, which corresponds to Merops family M56, are also found in a number of other bacterial genome sequences.


Pssm-ID: 428523  Cd Length: 296  Bit Score: 98.31  E-value: 1.31e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201  27 KRYFNYMLNYKVWYLTLLAGLIPFIPI-KFSLFKFNNVN---NQAPTVESKSHDLNHNINTTkpiqefATDIHKFNWDSI 102
Cdd:pfam05569  25 RRYLGAIWAYALWLIVPLALLAPFIPEsALSNFTTSAIFmarSKAPINSTAPKVIDDQASPL------ATPFPVVAWSTI 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201 103 DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLylNENEKNKIDTILF----NHQYKKNIVIRKAETIQSPITFWYGKY 178
Cdd:pfam05569  99 LKILLLLWIVGALILLLYMIAAYLVFRQRRVRRL--GSLRAHELDDILKeakeDMGIKRPITISLSSNIDSPAVLGLWKP 176

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201 179 IILIPSSYFKSVIDKRLKYIILHEYAHAKNRDtlhlIIFNIFS----IIMSYNPLVHIVKRKIIHDNEVEADRFVLNNIN 254
Cdd:pfam05569 177 RIVLPADFDTRLSGEEIDYILAHELSHLKRGD----LIINLLVavlqCLHWFNPLVHLAFRKIRIDQELACDAAVLARLH 252

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....
gi 1437472201 255 KNEFKTYAESIMDSVLNVPFFNKNILSHSFNGKKSLLKRRLINI 298
Cdd:pfam05569 253 PHERKEYGRTLLKLLAGPSNHIVPVACVWLAGAKSALKERIMML 296
M56_BlaR1_MecR1_like cd07341
Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; ...
 109-302 2.50e-22

Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; This family contains peptidase M56, which includes zinc metalloprotease domain in MecR1 as well as BlaR1. MecR1 is a transmembrane beta-lactam sensor/signal transducer protein that regulates the expression of an altered penicillin-binding protein PBP2a, which resists inactivation by beta-lactam antibiotics, in methicillin-resistant Staphylococcus aureus (MRSA). BlaR1 regulates the inducible expression of a class A beta-lactamase that hydrolytically destroys certain ?-lactam antibiotics in MRSA. Both, MecR1 and BlaR1, are transmembrane proteins that consist of four transmembrane helices, a cytoplasmic zinc protease domain, and the soluble C-terminal extracellular sensor domain, and are highly similar in sequence and function. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. All members contain the zinc metalloprotease motif (HEXXH). Homologs of this peptidase domain are also found in a number of other bacterial genome sequences, most of which are as yet uncharacterized.


Pssm-ID: 320700 [Multi-domain]  Cd Length: 187  Bit Score: 92.39  E-value: 2.50e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201 109 IWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILfnhQYKKNIVIRKAETIQSPITFWYGKYIILIPSSyFK 188
Cdd:cd07341     1 IWLAGALLLLLRLLRGLLRLRRLRRRAEPVPDSLLLELARRL---GLRRSVRLSVSALVASPMVVGLFRPVILLPEG-LL 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1437472201 189 SVIDKRLKYIILHEYAHAKNRDTLHLIIFNIFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNniNKNEFKTYAESIMDs 268
Cdd:cd07341    77 EGSPEELRAILLHELAHIRRRDLLVNLLQRLLEALFWFNPLVWLLSRRLRLERELACDEAVLA--ALGDKEDYAEALLR- 153

                         170       180       190
                  ....*....|....*....|....*....|....
gi 1437472201 269 VLNVPFFNKNILSHSFNGKKSLLKRRLINIKEAN 302
Cdd:cd07341   154 LAERRSQPPPALALALAGSKSLLKRRIKRILKKK 187
M56_BlaR1_MecR1_like cd07326
Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; ...
 198-248 9.82e-07

Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; This family contains peptidase M56, which includes zinc metalloprotease domain in MecR1 as well as BlaR1. MecR1 is a transmembrane beta-lactam sensor/signal transducer protein that regulates the expression of an altered penicillin-binding protein PBP2a, which resists inactivation by beta-lactam antibiotics, in methicillin-resistant Staphylococcus aureus (MRSA). BlaR1 regulates the inducible expression of a class A beta-lactamase that hydrolytically destroys certain ?-lactam antibiotics in MRSA. Both, MecR1 and BlaR1, are transmembrane proteins that consist of four transmembrane helices, a cytoplasmic zinc protease domain, and the soluble C-terminal extracellular sensor domain, and are highly similar in sequence and function. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. All members contain the zinc metalloprotease motif (HEXXH). Homologs of this peptidase domain are also found in a number of other bacterial genome sequences, most of which are as yet uncharacterized.


Pssm-ID: 320685 [Multi-domain]  Cd Length: 165  Bit Score: 48.07  E-value: 9.82e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1437472201 198 IILHEYAHAKNRDTLHLIIFNIFSIIMSYNPLVHIVKRKIIHDNEVEADRF 248
Cdd:cd07326    67 VLAHERAHLRRRDPLLLLLASALARALPFLPLLRRLAAAYRLLRELAADDA 117
M48B_HtpX_like cd07337
Peptidase M48 subfamily B HtpX-like membrane-bound metallopeptidase; This HtpX family of ...
 194-248 6.44e-03

Peptidase M48 subfamily B HtpX-like membrane-bound metallopeptidase; This HtpX family of peptidase M48 subfamily B includes uncharacterized HtpX homologs and consists of proteins smaller than Ste24p, with homology restricted to the C-terminal half of Ste24p. HtpX expression is controlled by the Cpx stress response system, which senses abnormal membrane proteins. HtpX participates in the proteolytic quality control of these misfolded proteins by undergoing self-degradation and collaborating with FtsH, a membrane-bound and ATP-dependent protease, to eliminate them. HtpX, a zinc metalloprotease with an active site motif HEXXH, has an FtsH-like topology, and is capable of introducing endoproteolytic cleavages into SecY (also an FtsH substrate). However, HtpX does not have an ATPase activity and will only act against cytoplasmic regions of a target membrane protein. Thus, HtpX and FtsH have overlapping and/or complementary functions, which are especially important at high temperature; in E. coli and Xylella fastidiosa, HtpX is heat-inducible, while in Streptococcus gordonii it is not.


Pssm-ID: 320696 [Multi-domain]  Cd Length: 203  Bit Score: 37.29  E-value: 6.44e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1437472201 194 RLKYIILHEYAHAKNRDTLHLIIFNIFSIIMSYNPLVHIVKRKIIHD---------NEVEADRF 248
Cdd:cd07337    93 ELKGILAHELGHLSHKDTDYLLLIFVLLLLAAIWTKLGTLLIFVWIRllvmfssrkAEYRADAF 156
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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