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Conserved domains on  [gi|1403592989|emb|SQI22722|]
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ADA regulatory protein [Salmonella enterica subsp. arizonae]

Protein Classification

bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada( domain architecture ID 11487795)

bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada has a functional switch mechanism from a DNA methyltransferase to a transcriptional regulator

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK15435 PRK15435
bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;
1-352 0e+00

bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;


:

Pssm-ID: 185333 [Multi-domain]  Cd Length: 353  Bit Score: 639.15  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989   1 MKKALLTDDECWQRVQARDASADGCFVFAVRTTGIFCRPSCRSKRALRKNVRFFANAQQALDAGFRPCKRCQPDDARAQR 80
Cdd:PRK15435    2 KNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRARHALRENVRFYANASEALAAGFRPCKRCQPDKANPQQ 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989  81 RQLDKIACACRLLEQETPVTLASLAQAVAMSPFHLHRLFKASTGMTPKGWQQAWRARRLREALAKGERVTAAIYRAGFPD 160
Cdd:PRK15435   82 HRLDKITHACRLLEQETPVTLEALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLREALAKGESVTTSILNAGFPD 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 161 SSSYYRHADQTLGMTAKQFRKGGDNVSVRYALTDWIYGRCLVAESERGICAILPDDSDDALLAELHTLFPAARHEPADVR 240
Cdd:PRK15435  162 SSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDDDAALISELQQMFPAADNAPADLT 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 241 FQQRVQQVVAAINTRDVLLSLPLDIQGTAFQQQVWQALRAIPCGETVSYQQLAARIGKPTAVRAVASACGANKLAMVIPC 320
Cdd:PRK15435  242 FQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPC 321

                         330       340       350
                  ....*....|....*....|....*....|..
gi 1403592989 321 HRVVGRDGALSGYRWGVSRKAQLLKREAQKEE 352
Cdd:PRK15435  322 HRVVRGDGALSGYRWGVSRKAQLLRREAENEE 353
 
Name Accession Description Interval E-value
PRK15435 PRK15435
bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;
1-352 0e+00

bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;


Pssm-ID: 185333 [Multi-domain]  Cd Length: 353  Bit Score: 639.15  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989   1 MKKALLTDDECWQRVQARDASADGCFVFAVRTTGIFCRPSCRSKRALRKNVRFFANAQQALDAGFRPCKRCQPDDARAQR 80
Cdd:PRK15435    2 KNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRARHALRENVRFYANASEALAAGFRPCKRCQPDKANPQQ 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989  81 RQLDKIACACRLLEQETPVTLASLAQAVAMSPFHLHRLFKASTGMTPKGWQQAWRARRLREALAKGERVTAAIYRAGFPD 160
Cdd:PRK15435   82 HRLDKITHACRLLEQETPVTLEALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLREALAKGESVTTSILNAGFPD 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 161 SSSYYRHADQTLGMTAKQFRKGGDNVSVRYALTDWIYGRCLVAESERGICAILPDDSDDALLAELHTLFPAARHEPADVR 240
Cdd:PRK15435  162 SSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDDDAALISELQQMFPAADNAPADLT 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 241 FQQRVQQVVAAINTRDVLLSLPLDIQGTAFQQQVWQALRAIPCGETVSYQQLAARIGKPTAVRAVASACGANKLAMVIPC 320
Cdd:PRK15435  242 FQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPC 321

                         330       340       350
                  ....*....|....*....|....*....|..
gi 1403592989 321 HRVVGRDGALSGYRWGVSRKAQLLKREAQKEE 352
Cdd:PRK15435  322 HRVVRGDGALSGYRWGVSRKAQLLRREAENEE 353
AdaA COG2169
Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), ...
 1-352 3.98e-146

Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), contains Zn-binding and two AraC-type DNA-binding domains [Replication, recombination and repair];


Pssm-ID: 441772 [Multi-domain]  Cd Length: 358  Bit Score: 416.76  E-value: 3.98e-146
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989   1 MKKALLTDDECWQRVQARDASADGCFVFAVRTTGIFCRPSCRSKRALRKNVRFFANAQQALDAGFRPCKRCQPDDARAQR 80
Cdd:COG2169     1 MTTDLLDDDERWQAVLARDARFDGRFFYGVKTTGIYCRPSCPARKPKRENVRFFATAAAAEAAGFRPCKRCRPDLAPGSP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989  81 RQLDKIACACRLLEQ--ETPVTLASLAQAVAMSPFHLHRLFKASTGMTPKGWQQAWRARRLREALAKGERVTAAIYRAGF 158
Cdd:COG2169    81 PRADLVARACRLIEAgaEDRPSLEDLAARLGLSPRHLRRLFKAHTGVTPKAYARARRLLRARQLLQTGLSVTDAAYAAGF 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 159 PDSSSYYRHADQTLGMTAKQFRKGGDNVSVRYALTDWIYGRCLVAESERGICAILPDDSDDALLAELHTLFPAARHEPAD 238
Cdd:COG2169   161 GSLSRFYEAFKKLLGMTPSAYRRGGAGAAIRFAPTPCSLGLLLVAASARGVCAILLGDDPEALLRDLQDRFPAAELIGGD 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 239 VRFQQRVQQVVAAINTRDVLLSLPLDIQGTAFQQQVWQALRAIPCGETVSYQQLAARIGKPTAVRAVASACGANKLAMVI 318
Cdd:COG2169   241 AAFEQLVAEVVGFVEGPLLGLDLPLDLRGTAFQQRVWQALRAIPAGETASYAEIAARIGAPKAVRAVAAACAANQLAVAI 320

                         330       340       350
                  ....*....|....*....|....*....|....
gi 1403592989 319 PCHRVVGRDGALSGYRWGVSRKAQLLKREAQKEE 352
Cdd:COG2169   321 PCHRVVRADGALSGYRWGVERKRALLEREAAAAA 354
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
 268-347 1.14e-43

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 145.53  E-value: 1.14e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 268 TAFQQQVWQALRAIPCGETVSYQQLAARIGKPTAVRAVASACGANKLAMVIPCHRVVGRDGALSGYRWGVSRKAQLLKRE 347
Cdd:TIGR00589   1 TPFQQKVWKALRTIPYGETKSYGQLAKAIGNPKAARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKEFLLEHE 80
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
 269-349 2.79e-42

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 141.73  E-value: 2.79e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 269 AFQQQVWQALRAIPCGETVSYQQLAARIGKPTAVRAVASACGANKLAMVIPCHRVVGRDGALSGYRWGVSRKAQLLKREA 348
Cdd:pfam01035   1 PFQRRVWEALRQIPYGKTTTYGEIAKLLGRPKAARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRALLELEG 80


                  .
gi 1403592989 349 Q 349
Cdd:pfam01035  81 V 81
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
 270-347 1.20e-38

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 132.22  E-value: 1.20e-38
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1403592989 270 FQQQVWQALRAIPCGETVSYQQLAARIGKPTAVRAVASACGANKLAMVIPCHRVVGRDGALSGYRWGVSRKAQLLKRE 347
Cdd:cd06445     1 FQRRVWEALRQIPYGEVTTYGQIAKLAGTPKAARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELLELE 78
HTH_ARAC smart00342
helix_turn_helix, arabinose operon control protein;
98-180 1.73e-20

helix_turn_helix, arabinose operon control protein;


Pssm-ID: 197666 [Multi-domain]  Cd Length: 84  Bit Score: 84.53  E-value: 1.73e-20
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989   98 PVTLASLAQAVAMSPFHLHRLFKASTGMTPKGWQQAWRARRLREALAKGER-VTAAIYRAGFPDSSSYYRHADQTLGMTA 176
Cdd:smart00342   1 PLTLEDLAEALGVSPRHLQRLFKKETGTTPKQYLRDRRLERARRLLRDTDLsVTEIALRVGFSSQSYFSRAFKKLFGVTP 80


                   ....
gi 1403592989  177 KQFR 180
Cdd:smart00342  81 SEYR 84
 
Name Accession Description Interval E-value
PRK15435 PRK15435
bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;
1-352 0e+00

bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;


Pssm-ID: 185333 [Multi-domain]  Cd Length: 353  Bit Score: 639.15  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989   1 MKKALLTDDECWQRVQARDASADGCFVFAVRTTGIFCRPSCRSKRALRKNVRFFANAQQALDAGFRPCKRCQPDDARAQR 80
Cdd:PRK15435    2 KNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRARHALRENVRFYANASEALAAGFRPCKRCQPDKANPQQ 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989  81 RQLDKIACACRLLEQETPVTLASLAQAVAMSPFHLHRLFKASTGMTPKGWQQAWRARRLREALAKGERVTAAIYRAGFPD 160
Cdd:PRK15435   82 HRLDKITHACRLLEQETPVTLEALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLREALAKGESVTTSILNAGFPD 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 161 SSSYYRHADQTLGMTAKQFRKGGDNVSVRYALTDWIYGRCLVAESERGICAILPDDSDDALLAELHTLFPAARHEPADVR 240
Cdd:PRK15435  162 SSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDDDAALISELQQMFPAADNAPADLT 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 241 FQQRVQQVVAAINTRDVLLSLPLDIQGTAFQQQVWQALRAIPCGETVSYQQLAARIGKPTAVRAVASACGANKLAMVIPC 320
Cdd:PRK15435  242 FQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPC 321

                         330       340       350
                  ....*....|....*....|....*....|..
gi 1403592989 321 HRVVGRDGALSGYRWGVSRKAQLLKREAQKEE 352
Cdd:PRK15435  322 HRVVRGDGALSGYRWGVSRKAQLLRREAENEE 353
AdaA COG2169
Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), ...
 1-352 3.98e-146

Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), contains Zn-binding and two AraC-type DNA-binding domains [Replication, recombination and repair];


Pssm-ID: 441772 [Multi-domain]  Cd Length: 358  Bit Score: 416.76  E-value: 3.98e-146
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989   1 MKKALLTDDECWQRVQARDASADGCFVFAVRTTGIFCRPSCRSKRALRKNVRFFANAQQALDAGFRPCKRCQPDDARAQR 80
Cdd:COG2169     1 MTTDLLDDDERWQAVLARDARFDGRFFYGVKTTGIYCRPSCPARKPKRENVRFFATAAAAEAAGFRPCKRCRPDLAPGSP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989  81 RQLDKIACACRLLEQ--ETPVTLASLAQAVAMSPFHLHRLFKASTGMTPKGWQQAWRARRLREALAKGERVTAAIYRAGF 158
Cdd:COG2169    81 PRADLVARACRLIEAgaEDRPSLEDLAARLGLSPRHLRRLFKAHTGVTPKAYARARRLLRARQLLQTGLSVTDAAYAAGF 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 159 PDSSSYYRHADQTLGMTAKQFRKGGDNVSVRYALTDWIYGRCLVAESERGICAILPDDSDDALLAELHTLFPAARHEPAD 238
Cdd:COG2169   161 GSLSRFYEAFKKLLGMTPSAYRRGGAGAAIRFAPTPCSLGLLLVAASARGVCAILLGDDPEALLRDLQDRFPAAELIGGD 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 239 VRFQQRVQQVVAAINTRDVLLSLPLDIQGTAFQQQVWQALRAIPCGETVSYQQLAARIGKPTAVRAVASACGANKLAMVI 318
Cdd:COG2169   241 AAFEQLVAEVVGFVEGPLLGLDLPLDLRGTAFQQRVWQALRAIPAGETASYAEIAARIGAPKAVRAVAAACAANQLAVAI 320

                         330       340       350
                  ....*....|....*....|....*....|....
gi 1403592989 319 PCHRVVGRDGALSGYRWGVSRKAQLLKREAQKEE 352
Cdd:COG2169   321 PCHRVVRADGALSGYRWGVERKRALLEREAAAAA 354
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 198-348 9.20e-64

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 200.10  E-value: 9.20e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 198 GRCLVAESERGICAI-LPDDSDDALLAElhtlFPAARHEPADVrFQQRVQQVVAAINTRDVLLSLPLDIQGTAFQQQVWQ 276
Cdd:COG0350    12 GPLLIAATDRGLCALsFGDDREEALLAR----FPAALREDPPL-LAEAARQLDAYFAGERKDFDLPLDLRGTPFQRRVWE 86

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1403592989 277 ALRAIPCGETVSYQQLAARIGKPTAVRAVASACGANKLAMVIPCHRVVGRDGALSGYRWGVSRKAQLLKREA 348
Cdd:COG0350    87 ALRKIPYGETVTYGELARAIGRPKAARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLELEG 158
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
 268-347 1.14e-43

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 145.53  E-value: 1.14e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 268 TAFQQQVWQALRAIPCGETVSYQQLAARIGKPTAVRAVASACGANKLAMVIPCHRVVGRDGALSGYRWGVSRKAQLLKRE 347
Cdd:TIGR00589   1 TPFQQKVWKALRTIPYGETKSYGQLAKAIGNPKAARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKEFLLEHE 80
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
 269-349 2.79e-42

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 141.73  E-value: 2.79e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 269 AFQQQVWQALRAIPCGETVSYQQLAARIGKPTAVRAVASACGANKLAMVIPCHRVVGRDGALSGYRWGVSRKAQLLKREA 348
Cdd:pfam01035   1 PFQRRVWEALRQIPYGKTTTYGEIAKLLGRPKAARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRALLELEG 80


                  .
gi 1403592989 349 Q 349
Cdd:pfam01035  81 V 81
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
 270-347 1.20e-38

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 132.22  E-value: 1.20e-38
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1403592989 270 FQQQVWQALRAIPCGETVSYQQLAARIGKPTAVRAVASACGANKLAMVIPCHRVVGRDGALSGYRWGVSRKAQLLKRE 347
Cdd:cd06445     1 FQRRVWEALRQIPYGEVTTYGQIAKLAGTPKAARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELLELE 78
Ada_Zn_binding pfam02805
Metal binding domain of Ada; The Escherichia coli Ada protein repairs O6-methylguanine ...
 10-71 5.78e-34

Metal binding domain of Ada; The Escherichia coli Ada protein repairs O6-methylguanine residues and methyl phosphotriesters in DNA by direct transfer of the methyl group to a cysteine residue. This domain contains four conserved cysteines that form a zinc binding site. One of these cysteines is a methyl group acceptor. The methylated domain can then specifically bind to the ada box on a DNA duplex.


Pssm-ID: 460701 [Multi-domain]  Cd Length: 62  Bit Score: 119.47  E-value: 5.78e-34
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1403592989  10 ECWQRVQARDASADGCFVFAVRTTGIFCRPSCRSKRALRKNVRFFANAQQALDAGFRPCKRC 71
Cdd:pfam02805   1 ARWQAVLARDPRADGSFFYAVKTTGIYCRPSCPARLPKRENVRFFDTAAEAEAAGFRPCKRC 62
PRK10286 PRK10286
methylated-DNA--[protein]-cysteine S-methyltransferase;
257-347 2.20e-32

methylated-DNA--[protein]-cysteine S-methyltransferase;


Pssm-ID: 182355 [Multi-domain]  Cd Length: 171  Bit Score: 119.20  E-value: 2.20e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 257 VLLSLPLDIQGTAFQQQVWQALRAIPCGETVSYQQLAARIGKPTAVRAVASACGANKLAMVIPCHRVVGRDGALSGYRWG 336
Cdd:PRK10286   76 IIDTLPTATGGTPFQREVWQTLRTIPCGQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGG 155

                          90
                  ....*....|.
gi 1403592989 337 VSRKAQLLKRE 347
Cdd:PRK10286  156 VQRKEWLLRHE 166
PRK00901 PRK00901
methylated-DNA--protein-cysteine methyltransferase; Provisional
 261-350 1.30e-31

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 234860 [Multi-domain]  Cd Length: 155  Bit Score: 116.30  E-value: 1.30e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 261 LPLDIQGTAFQQQVWQALRAIPCGETVSYQQLAARIGKPTAVRAVASACGANKLAMVIPCHRVVGRDGALSGYRWGVSRK 340
Cdd:PRK00901   65 LPLAPQGTEFQKKVWKALQEIPYGETRSYKEIAVNIGNPKACRAVGLANNKNPIPIFIPCHRVIGANGKLVGYAGGLDIK 144

                          90
                  ....*....|
gi 1403592989 341 AQLLKREAQK 350
Cdd:PRK00901  145 EKLLKLEKEN 154
HTH_ARAC smart00342
helix_turn_helix, arabinose operon control protein;
98-180 1.73e-20

helix_turn_helix, arabinose operon control protein;


Pssm-ID: 197666 [Multi-domain]  Cd Length: 84  Bit Score: 84.53  E-value: 1.73e-20
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989   98 PVTLASLAQAVAMSPFHLHRLFKASTGMTPKGWQQAWRARRLREALAKGER-VTAAIYRAGFPDSSSYYRHADQTLGMTA 176
Cdd:smart00342   1 PLTLEDLAEALGVSPRHLQRLFKKETGTTPKQYLRDRRLERARRLLRDTDLsVTEIALRVGFSSQSYFSRAFKKLFGVTP 80


                   ....
gi 1403592989  177 KQFR 180
Cdd:smart00342  81 SEYR 84
Methyltransf_1N pfam02870
6-O-methylguanine DNA methyltransferase, ribonuclease-like domain; This entry represents the ...
 187-265 1.49e-19

6-O-methylguanine DNA methyltransferase, ribonuclease-like domain; This entry represents the N-terminal ribonuclease-like domain associated with 6-O-methylguanine DNA methyltransferase activity. The repair of DNA containing O6-alkylated guanine is carried out by DNA-[protein]-cysteine S-methyltransferase (also known as O-6-methylguanine-DNA-alkyltransferase)


Pssm-ID: 397139 [Multi-domain]  Cd Length: 77  Bit Score: 81.64  E-value: 1.49e-19
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1403592989 187 SVRYALTDWIYGRCLVAESERGICAILPDDSDDALLAELHTLFPAARHEPADVRFQQRVQQVVAAINTrdVLLSLPLDI 265
Cdd:pfam02870   1 ALYYTLIDSPLGRLLLAGDERGLTAIDFLDKDYALRKELPKVLPQPELLPALALLVQALEEYFAGELK--PEFTLPLDQ 77
Atl1 COG3695
Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like ...
 270-347 4.44e-16

Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like DNA-binding domain [Transcription];


Pssm-ID: 442910  Cd Length: 104  Bit Score: 72.91  E-value: 4.44e-16
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1403592989 270 FQQQVWQALRAIPCGETVSYQQLAARIGKPTAVRAVASACGANKLAMVIPCHRVVGRDGALS-GYRWGVSRKAQLLKRE 347
Cdd:COG3695     6 FYERVYEVVAQIPPGRVATYGDIAALAGLPRGARQVGRALRALPEGSDLPWHRVVNADGRLSpGHAGGAEEQRELLEAE 84
HTH_18 pfam12833
Helix-turn-helix domain;
104-181 4.94e-15

Helix-turn-helix domain;


Pssm-ID: 432818 [Multi-domain]  Cd Length: 81  Bit Score: 69.54  E-value: 4.94e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 104 LAQAVAMSPFHLHRLFKASTGMTPKGWQQAWRARRLREALAK--GERVTAAIYRAGFPDSSSYYRHADQTLGMTAKQFRK 181
Cdd:pfam12833   1 LAAALGMSPRTLSRLFKRELGLSPKEYLRRLRLERARRLLLEdtGLSVAEIALALGFSDASHFSRAFRRLFGLTPSEYRR 80
AraC COG2207
AraC-type DNA-binding domain and AraC-containing proteins [Transcription];
75-182 5.57e-15

AraC-type DNA-binding domain and AraC-containing proteins [Transcription];


Pssm-ID: 441809 [Multi-domain]  Cd Length: 258  Bit Score: 74.05  E-value: 5.57e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989  75 DARAQRRQLDKIACACRLLEQETPVTLASLAQAVAMSPFHLHRLFKASTGMTPKGWQQAWRARRLREALAKGER-VTAAI 153
Cdd:COG2207   145 LLLLLLLLLLLLLLLLLLLLLLLLLTLEELARELGLSPRTLSRLFKEETGTSPKQYLRELRLERAKRLLAETDLsISEIA 224

                          90       100
                  ....*....|....*....|....*....
gi 1403592989 154 YRAGFPDSSSYYRHADQTLGMTAKQFRKG 182
Cdd:COG2207   225 YELGFSSQSHFSRAFKKRFGVTPSEYRKR 253
GlxA COG4977
Transcriptional regulator GlxA, contains an amidase domain and an AraC-type DNA-binding HTH ...
 84-181 7.07e-13

Transcriptional regulator GlxA, contains an amidase domain and an AraC-type DNA-binding HTH domain [Transcription];


Pssm-ID: 444002 [Multi-domain]  Cd Length: 318  Bit Score: 68.26  E-value: 7.07e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989  84 DKIACACRLLEQ--ETPVTLASLAQAVAMSPFHLHRLFKASTGMTPKGWQQAWRARRLREALAKGER-VTAAIYRAGFPD 160
Cdd:COG4977   210 PRLARAQAWMEAnlEEPLSVDELARRAGMSPRTLERRFRAATGTTPARYLQRLRLERARRLLETTDLsIEEIAAACGFGS 289

                          90       100
                  ....*....|....*....|.
gi 1403592989 161 SSSYYRHADQTLGMTAKQFRK 181
Cdd:COG4977   290 ASHFRRAFRRRFGVSPSAYRR 310
ftrA PRK09393
transcriptional activator FtrA; Provisional
 98-181 1.22e-10

transcriptional activator FtrA; Provisional


Pssm-ID: 181818 [Multi-domain]  Cd Length: 322  Bit Score: 61.90  E-value: 1.22e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989  98 PVTLASLAQAVAMSPFHLHRLFKASTGMTPKGWQQAWRARRLREALAKGERVTAAIY-RAGFPDSSSYYRHADQTLGMTA 176
Cdd:PRK09393  234 PHTVASLAARAAMSPRTFLRRFEAATGMTPAEWLLRERLARARDLLESSALSIDQIAeRAGFGSEESLRHHFRRRAATSP 313


                  ....*
gi 1403592989 177 KQFRK 181
Cdd:PRK09393  314 AAYRK 318
HTH_AraC pfam00165
Bacterial regulatory helix-turn-helix proteins, AraC family; In the absence of arabinose, the ...
 97-132 1.23e-07

Bacterial regulatory helix-turn-helix proteins, AraC family; In the absence of arabinose, the N-terminal arm of AraC binds to the DNA binding domain (pfam00165) and helps to hold the two DNA binding domains in a relative orientation that favours DNA looping. In the presence of arabinose, the arms bind over the arabinose on the dimerization domain, thus freeing the DNA-binding domains. The freed DNA-binding domains are then able to assume a conformation suitable for binding to the adjacent DNA sites that are utilized when AraC activates transcription, and hence AraC ceases looping the DNA when arabinose is added.


Pssm-ID: 425497 [Multi-domain]  Cd Length: 42  Bit Score: 47.53  E-value: 1.23e-07
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 1403592989  97 TPVTLASLAQAVAMSPFHLHRLFKASTGMTPKGWQQ 132
Cdd:pfam00165   7 TNLTIADIADELGFSRSYFSRLFKKYTGVTPSQYRH 42
PRK03887 PRK03887
methylated-DNA--protein-cysteine methyltransferase; Provisional
 268-351 1.99e-07

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 167628 [Multi-domain]  Cd Length: 175  Bit Score: 50.50  E-value: 1.99e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989 268 TAFQQQVWQAL-RAIPCGETVSYQQLAARIGkpTAVRAVASACGANKLAMVIPCHRVVGRDGaLSGYRWGVSRKAQLLKR 346
Cdd:PRK03887   91 TPFERKVYEWLtKNVKRGEVITYGELAKALN--TSPRAVGGAMKRNPYPIIVPCHRVVGRKN-PGLYTPKPEYKKFLLEV 167


                  ....*
gi 1403592989 347 EAQKE 351
Cdd:PRK03887  168 EGVKE 172
PRK10371 PRK10371
transcriptional regulator MelR;
98-181 9.21e-04

transcriptional regulator MelR;


Pssm-ID: 182416 [Multi-domain]  Cd Length: 302  Bit Score: 40.57  E-value: 9.21e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989  98 PVTLASLAQAVAMSPFHLHRLFKASTGMTPKGWQQAWRARRLREALAKGERVTAAI-YRAGFPDSSSYYRHADQTLGMTA 176
Cdd:PRK10371  207 ALTINDVAEHVKLNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIaLTAGFRSSSRFYSTFGKYVGMSP 286


                  ....*
gi 1403592989 177 KQFRK 181
Cdd:PRK10371  287 QQYRK 291
PRK09685 PRK09685
DNA-binding transcriptional activator FeaR; Provisional
 57-182 1.94e-03

DNA-binding transcriptional activator FeaR; Provisional


Pssm-ID: 236612 [Multi-domain]  Cd Length: 302  Bit Score: 39.63  E-value: 1.94e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1403592989  57 AQQALDAGFRPCKRCQPDDARAQRRQLDKIACACRLLEQETPVTLASLAQAVAMSPFHLHRLFkASTGMTPKGWQQAWRA 136
Cdd:PRK09685  173 LLEALIALLRPALHQRESVQPRRERQFQKVVALIDQSIQEEILRPEWIAGELGISVRSLYRLF-AEQGLVVAQYIRNRRL 251

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1403592989 137 RRLREAL---AKGERVTAAIYRAGFPDSSSYYRHADQTLGMTAKQFRKG 182
Cdd:PRK09685  252 DRCADDLrpaADDEKITSIAYKWGFSDSSHFSTAFKQRFGVSPGEYRRK 300
AraC COG2207
AraC-type DNA-binding domain and AraC-containing proteins [Transcription];
85-138 3.49e-03

AraC-type DNA-binding domain and AraC-containing proteins [Transcription];


Pssm-ID: 441809 [Multi-domain]  Cd Length: 258  Bit Score: 38.61  E-value: 3.49e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1403592989  85 KIACACRLLeQETPVTLASLAQAVAM-SPFHLHRLFKASTGMTPKGWQQAWRARR 138
Cdd:COG2207   205 RLERAKRLL-AETDLSISEIAYELGFsSQSHFSRAFKKRFGVTPSEYRKRLRARA 258
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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