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Conserved domains on  [gi|23396475|sp|Q9VF08|]
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RecName: Full=Mitochondrial import inner membrane translocase subunit Tim16; AltName: Full=Protein black pearl

Protein Classification

J domain-containing protein( domain architecture ID 84)

J domain-containing protein similar to molecular chaperone DnaJ, a protein that plays crucial roles in protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of Hsp70

CATH:  1.10.287.110
Gene Ontology:  GO:0006457
PubMed:  35729039|9644977|8016869|15170475
SCOP:  4000605

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DnaJ super family cl02542
DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and ...
 1-117 3.76e-33

DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation. They act primarily by stimulating the ATPase activity of Hsp70s, an important chaperonine family. Hsp40 proteins are characterized by the presence of a J domain, which mediates the interaction with Hsp70. They may contain other domains as well, and the architectures provide a means of classification.


The actual alignment was detected with superfamily member pfam03656:

Pssm-ID: 413365  Cd Length: 127  Bit Score: 113.13  E-value: 3.76e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23396475     1 MAKYIA-QIIVLGAQAVGRAFTKALRQEIAASQEAArrAGGGKQGDKSAESNLRTGMTLEEAKQILNIDDPKNVDAITKN 79
Cdd:pfam03656   1 MAHRAAiQVIITGTQVVGKAFARAYRQEAAAQRVAA--GAGTNASRRGTAKSNYGGISLDESCQILNVEEDLNMEEVNKR 78

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 23396475    80 YEHLFQVNERSKGGSFYIQSKVFRAKERLDHEIKAHEQ 117
Cdd:pfam03656  79 YEHLFEVNDKSKGGSFYLQSKVYRAKERLDEELAIREK 116
 
Name Accession Description Interval E-value
Pam16 pfam03656
Pam16; The Pam16 protein is the fifth essential subunit of the pre-sequence ...
 1-117 3.76e-33

Pam16; The Pam16 protein is the fifth essential subunit of the pre-sequence translocase-associated protein import motor (PAM). In Saccharomyces cerevisiae, Pam16 is required for preprotein translocation into the matrix, but not for protein insertion into the inner membrane. Pam16 has a degenerate J domain. J-domain proteins play important regulatory roles as co-chaperones, recruiting Hsp70 partners and accelerating the ATP-hydrolysis step of the chaperone cycle. Pam16's J-like domain strongly interacts with Pam18's J domain, leading to a productive interaction of Pam18 with mtHsp70 at the mitochondria import channel. Pam18 stimulates the ATPase activity of mtHsp70.


Pssm-ID: 252088  Cd Length: 127  Bit Score: 113.13  E-value: 3.76e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23396475     1 MAKYIA-QIIVLGAQAVGRAFTKALRQEIAASQEAArrAGGGKQGDKSAESNLRTGMTLEEAKQILNIDDPKNVDAITKN 79
Cdd:pfam03656   1 MAHRAAiQVIITGTQVVGKAFARAYRQEAAAQRVAA--GAGTNASRRGTAKSNYGGISLDESCQILNVEEDLNMEEVNKR 78

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 23396475    80 YEHLFQVNERSKGGSFYIQSKVFRAKERLDHEIKAHEQ 117
Cdd:pfam03656  79 YEHLFEVNDKSKGGSFYLQSKVYRAKERLDEELAIREK 116
PTZ00100 PTZ00100
DnaJ chaperone protein; Provisional
 56-108 4.21e-06

DnaJ chaperone protein; Provisional


Pssm-ID: 240265  Cd Length: 116  Bit Score: 42.92  E-value: 4.21e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 23396475   56 MTLEEAKQILNIDDPKNVDAITKNYEHLFQVNERSKGGSFYIQSKVFRAKERL 108
Cdd:PTZ00100  62 MSKSEAYKILNISPTASKERIREAHKQLMLRNHPDNGGSTYIASKVNEAKDLL 114
 
Name Accession Description Interval E-value
Pam16 pfam03656
Pam16; The Pam16 protein is the fifth essential subunit of the pre-sequence ...
 1-117 3.76e-33

Pam16; The Pam16 protein is the fifth essential subunit of the pre-sequence translocase-associated protein import motor (PAM). In Saccharomyces cerevisiae, Pam16 is required for preprotein translocation into the matrix, but not for protein insertion into the inner membrane. Pam16 has a degenerate J domain. J-domain proteins play important regulatory roles as co-chaperones, recruiting Hsp70 partners and accelerating the ATP-hydrolysis step of the chaperone cycle. Pam16's J-like domain strongly interacts with Pam18's J domain, leading to a productive interaction of Pam18 with mtHsp70 at the mitochondria import channel. Pam18 stimulates the ATPase activity of mtHsp70.


Pssm-ID: 252088  Cd Length: 127  Bit Score: 113.13  E-value: 3.76e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23396475     1 MAKYIA-QIIVLGAQAVGRAFTKALRQEIAASQEAArrAGGGKQGDKSAESNLRTGMTLEEAKQILNIDDPKNVDAITKN 79
Cdd:pfam03656   1 MAHRAAiQVIITGTQVVGKAFARAYRQEAAAQRVAA--GAGTNASRRGTAKSNYGGISLDESCQILNVEEDLNMEEVNKR 78

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 23396475    80 YEHLFQVNERSKGGSFYIQSKVFRAKERLDHEIKAHEQ 117
Cdd:pfam03656  79 YEHLFEVNDKSKGGSFYLQSKVYRAKERLDEELAIREK 116
PTZ00100 PTZ00100
DnaJ chaperone protein; Provisional
 56-108 4.21e-06

DnaJ chaperone protein; Provisional


Pssm-ID: 240265  Cd Length: 116  Bit Score: 42.92  E-value: 4.21e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 23396475   56 MTLEEAKQILNIDDPKNVDAITKNYEHLFQVNERSKGGSFYIQSKVFRAKERL 108
Cdd:PTZ00100  62 MSKSEAYKILNISPTASKERIREAHKQLMLRNHPDNGGSTYIASKVNEAKDLL 114
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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