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Conserved domains on  [gi|75160541|sp|Q8S924|]
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RecName: Full=Ubiquitin-like protein ATG12A; AltName: Full=Autophagy-related protein 12a; Short=APG12-like protein a; Short=AtAPG12a

Protein Classification

ubiquitin-like protein ATG12( domain architecture ID 10108002)

ubiquitin-like protein ATG12 is involved in autophagy vesicle formation and is localized at the developing autophagosome

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Ubl_ATG12 cd01612
ubiquitin-like (Ubl) domain found in autophagy-related protein 12 (ATG12); Autophagy is an ...
 13-95 1.08e-45

ubiquitin-like (Ubl) domain found in autophagy-related protein 12 (ATG12); Autophagy is an essential intracellular process that targets large protein complexes, bacterial pathogens, and organelles for degradation. The autophagy-related ubiquitin-like (Ubl) proteins such as ATG12 protein have a conserved Ubl fold structure and undergo a unique Ubl conjugation, a process essential for autophagosome formation. ATG12 is conjugated to ATG5 by multistep modifications of the E1-like (ubiquitin activating) enzyme ATG7, and the E2-like (ubiquitin conjugating) enzyme ATG10. The ATG12-ATG5 conjugate facilitates the lipidation of ATG8 and directs its correct subcellular localization. ATG12 is localized at the developing autophagosome.


:

Pssm-ID: 340454  Cd Length: 86  Bit Score: 141.50  E-value: 1.08e-45
                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 75160541 13 KVVVHLRATGGAPILKQSKFKIPGTDKFAKVIDFLRRQLH---SDSLFVYVNSAFSPNPDESVIDLYNNFGFDGKLVVNY 89
Cdd:cd01612  1 KVVVLFKAVGDAPILKQKKFKVSASQKFASVIDFLRKQLKlkpSESLFLYINQSFAPSPDEEVGDLYDCFGSNGKLVLNY 80


               ....*.
gi 75160541 90 ACSMAW 95
Cdd:cd01612 81 CKTPAW 86
 
Name Accession Description Interval E-value
Ubl_ATG12 cd01612
ubiquitin-like (Ubl) domain found in autophagy-related protein 12 (ATG12); Autophagy is an ...
 13-95 1.08e-45

ubiquitin-like (Ubl) domain found in autophagy-related protein 12 (ATG12); Autophagy is an essential intracellular process that targets large protein complexes, bacterial pathogens, and organelles for degradation. The autophagy-related ubiquitin-like (Ubl) proteins such as ATG12 protein have a conserved Ubl fold structure and undergo a unique Ubl conjugation, a process essential for autophagosome formation. ATG12 is conjugated to ATG5 by multistep modifications of the E1-like (ubiquitin activating) enzyme ATG7, and the E2-like (ubiquitin conjugating) enzyme ATG10. The ATG12-ATG5 conjugate facilitates the lipidation of ATG8 and directs its correct subcellular localization. ATG12 is localized at the developing autophagosome.


Pssm-ID: 340454  Cd Length: 86  Bit Score: 141.50  E-value: 1.08e-45
                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 75160541 13 KVVVHLRATGGAPILKQSKFKIPGTDKFAKVIDFLRRQLH---SDSLFVYVNSAFSPNPDESVIDLYNNFGFDGKLVVNY 89
Cdd:cd01612  1 KVVVLFKAVGDAPILKQKKFKVSASQKFASVIDFLRKQLKlkpSESLFLYINQSFAPSPDEEVGDLYDCFGSNGKLVLNY 80


               ....*.
gi 75160541 90 ACSMAW 95
Cdd:cd01612 81 CKTPAW 86
APG12 pfam04110
Ubiquitin-like autophagy protein Apg12; In yeast, 15 Apg proteins coordinate the formation of ...
 13-96 4.23e-44

Ubiquitin-like autophagy protein Apg12; In yeast, 15 Apg proteins coordinate the formation of autophagosomes. Autophagy is a bulk degradation process induced by starvation in eukaryotic cells. The Apg12 system is one of the ubiquitin-like protein conjugation systems conserved in eukaryotes. It was first discovered in yeast during systematic analyses of the apg mutants defective in autophagy. Covalent attachment of Apg12-Apg5 is essential for autophagy.


Pssm-ID: 397985  Cd Length: 87  Bit Score: 137.54  E-value: 4.23e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 75160541   13 KVVVHLRATGGAPILKQSKFKIPGTDKFAKVIDFLRRQLH---SDSLFVYVNSAFSPNPDESVIDLYNNFGFDGKLVVNY 89
Cdd:pfam04110  1 KITIRLRAIGDAPILKKSKFKVNPSQTFASVILFLKKFLKlqaSDSLFLYVNNSFAPSPDQIVGNLYECFGTDGKLVLNY 80


                 ....*..
gi 75160541   90 ACSMAWG 96
Cdd:pfam04110 81 CISVAWG 87
 
Name Accession Description Interval E-value
Ubl_ATG12 cd01612
ubiquitin-like (Ubl) domain found in autophagy-related protein 12 (ATG12); Autophagy is an ...
 13-95 1.08e-45

ubiquitin-like (Ubl) domain found in autophagy-related protein 12 (ATG12); Autophagy is an essential intracellular process that targets large protein complexes, bacterial pathogens, and organelles for degradation. The autophagy-related ubiquitin-like (Ubl) proteins such as ATG12 protein have a conserved Ubl fold structure and undergo a unique Ubl conjugation, a process essential for autophagosome formation. ATG12 is conjugated to ATG5 by multistep modifications of the E1-like (ubiquitin activating) enzyme ATG7, and the E2-like (ubiquitin conjugating) enzyme ATG10. The ATG12-ATG5 conjugate facilitates the lipidation of ATG8 and directs its correct subcellular localization. ATG12 is localized at the developing autophagosome.


Pssm-ID: 340454  Cd Length: 86  Bit Score: 141.50  E-value: 1.08e-45
                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 75160541 13 KVVVHLRATGGAPILKQSKFKIPGTDKFAKVIDFLRRQLH---SDSLFVYVNSAFSPNPDESVIDLYNNFGFDGKLVVNY 89
Cdd:cd01612  1 KVVVLFKAVGDAPILKQKKFKVSASQKFASVIDFLRKQLKlkpSESLFLYINQSFAPSPDEEVGDLYDCFGSNGKLVLNY 80


               ....*.
gi 75160541 90 ACSMAW 95
Cdd:cd01612 81 CKTPAW 86
APG12 pfam04110
Ubiquitin-like autophagy protein Apg12; In yeast, 15 Apg proteins coordinate the formation of ...
 13-96 4.23e-44

Ubiquitin-like autophagy protein Apg12; In yeast, 15 Apg proteins coordinate the formation of autophagosomes. Autophagy is a bulk degradation process induced by starvation in eukaryotic cells. The Apg12 system is one of the ubiquitin-like protein conjugation systems conserved in eukaryotes. It was first discovered in yeast during systematic analyses of the apg mutants defective in autophagy. Covalent attachment of Apg12-Apg5 is essential for autophagy.


Pssm-ID: 397985  Cd Length: 87  Bit Score: 137.54  E-value: 4.23e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 75160541   13 KVVVHLRATGGAPILKQSKFKIPGTDKFAKVIDFLRRQLH---SDSLFVYVNSAFSPNPDESVIDLYNNFGFDGKLVVNY 89
Cdd:pfam04110  1 KITIRLRAIGDAPILKKSKFKVNPSQTFASVILFLKKFLKlqaSDSLFLYVNNSFAPSPDQIVGNLYECFGTDGKLVLNY 80


                 ....*..
gi 75160541   90 ACSMAWG 96
Cdd:pfam04110 81 CISVAWG 87
Ubl_Autophagy_like cd01611
ubiquitin-like (Ubl) domain found in autophagy-related ubiquitin-like protein; Autophagy is an ...
 13-90 1.16e-10

ubiquitin-like (Ubl) domain found in autophagy-related ubiquitin-like protein; Autophagy is an essential intracellular process that targets large protein complexes, bacterial pathogens, and organelles for degradation. The autophagy-related ubiquitin-like proteins, such as Saccharomyces cerevisiae Atg8p, undergo a unique ubiquitin-like (Ubl) conjugation, a process essential for autophagosome formation. Ubiquitin is a protein modifier in eukaryotes that is involved in various cellular processes including transcriptional regulation, cell cycle control, and DNA repair. The ubiquitination process comprises a cascade of E1, E2 and E3 enzymes that results in a covalent bond between the C-terminus of ubiquitin and the epsilon-amino group of a substrate lysine. ATG8 family proteins undergo multistep modifications by the E1-like (ubiquitin activating) enzyme ATG7, and the E2-like (ubiquitin conjugating) enzyme ATG3. The mammalian ATG8 family is classified into three subfamilies: i) MAP1LC3 (microtubule associated protein 1 light chain 3) which includes MAP1LC3A, MAP1LC3B, MAP1LC3B2, and MAP1LC3C, ii) GABARAP (GABA type A receptor associated protein) which includes GABARAP, GABARAPL1, and GABARAPL3, and iii) GABARAPL2 (GABA type A receptor associated protein like 2), also known as GATE-16 (golgi-associated adenosine triphosphatase enhancer of 16 kDa).


Pssm-ID: 340453  Cd Length: 84  Bit Score: 52.82  E-value: 1.16e-10
                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 75160541 13 KVVVHLRATGGA--PILKQSKFKIPGTDKFAKVIDFLRRQLH---SDSLFVYVNSaFSPNPDESVIDLYNNFG-FDGKLV 86
Cdd:cd01611  1 KIPVILEKAGKTkiPILDKKKYLVPRDLTVGQLIDFIRKRLKlvaEEALFIFVNQ-FAPPTSAEMGTLYEEHGdEDGFLY 79


               ....
gi 75160541 87 VNYA 90
Cdd:cd01611 80 LHYS 83
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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