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Conserved domains on  [gi|218511918|sp|Q6BIN3|]
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RecName: Full=Antagonist of mitotic exit network protein 1

Protein Classification

leucine-rich repeat domain-containing protein; F-box protein( domain architecture ID 10174679)

leucine-rich repeat (LRR) domain-containing protein may participate in protein-protein interactions| F-box protein functions as the substrate-recognition component of a SCF (Skp1-Cullin-F-box protein) ubiquitin-protein ligase that is involved in ubiquitin-dependent degradation

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
AMN1 cd09293
Antagonist of mitotic exit network protein 1; Amn1 has been functionally characterized in ...
 262-487 2.90e-120

Antagonist of mitotic exit network protein 1; Amn1 has been functionally characterized in Saccharomyces cerevisiae as a component of the Antagonist of MEN pathway (AMEN). The AMEN network is activated by MEN (mitotic exit network) via an active Cdc14, and in turn switches off MEN. Amn1 constitutes one of the alternative mechanisms by which MEN may be disrupted. Specifically, Amn1 binds Tem1 (Termination of M-phase, a GTPase that belongs to the RAS superfamily), and disrupts its association with Cdc15, the primary downstream target. Amn1 is a leucine-rich repeat (LRR) protein, with 12 repeats in the S. cerevisiae ortholog. As a negative regulator of the signal transduction pathway MEN, overexpression of AMN1 slows the growth of wild type cells. The function of the vertebrate members of this family has not been determined experimentally, they have fewer LRRs that determine the extent of this model.


:

Pssm-ID: 187754 [Multi-domain]  Cd Length: 226  Bit Score: 353.17  E-value: 2.90e-120
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 218511918 262 KPSLFVFHKLFHAKQDIIELIKsHMDFSNLQWLEFYMCPKLLPTPEFLSCGTKIKKIVITGSKVIDDGFLSMVAKKCPNL 341
Cdd:cd09293    2 DPLLFILHKLGQITQSNISQLL-RILHSGLEWLELYMCPISDPPLDQLSNCNKLKKLILPGSKLIDDEGLIALAQSCPNL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 218511918 342 EVLDIRACELISDSGIYQIAKRCTKLTTVNFGRKNKGNLITDSSICILIRNNPNLKTVGLAGCHITDKTLWDLAIRCSDH 421
Cdd:cd09293   81 QVLDLRACENITDSGIVALATNCPKLQTINLGRHRNGHLITDVSLSALGKNCTFLQTVGFAGCDVTDKGVWELASGCSKS 160

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 218511918 422 LQRLSLNNCPHITNQSIPLILHSNLFKNISVLELRFANQITNFKPIIEFKRRQEFRGISILIEVCE 487
Cdd:cd09293  161 LERLSLNNCRNLTDQSIPAILASNYFPNLSVLEFRGCPLITDFSRIILFKLWQPRLNKPILVEWCE 226
 
Name Accession Description Interval E-value
AMN1 cd09293
Antagonist of mitotic exit network protein 1; Amn1 has been functionally characterized in ...
 262-487 2.90e-120

Antagonist of mitotic exit network protein 1; Amn1 has been functionally characterized in Saccharomyces cerevisiae as a component of the Antagonist of MEN pathway (AMEN). The AMEN network is activated by MEN (mitotic exit network) via an active Cdc14, and in turn switches off MEN. Amn1 constitutes one of the alternative mechanisms by which MEN may be disrupted. Specifically, Amn1 binds Tem1 (Termination of M-phase, a GTPase that belongs to the RAS superfamily), and disrupts its association with Cdc15, the primary downstream target. Amn1 is a leucine-rich repeat (LRR) protein, with 12 repeats in the S. cerevisiae ortholog. As a negative regulator of the signal transduction pathway MEN, overexpression of AMN1 slows the growth of wild type cells. The function of the vertebrate members of this family has not been determined experimentally, they have fewer LRRs that determine the extent of this model.


Pssm-ID: 187754 [Multi-domain]  Cd Length: 226  Bit Score: 353.17  E-value: 2.90e-120
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 218511918 262 KPSLFVFHKLFHAKQDIIELIKsHMDFSNLQWLEFYMCPKLLPTPEFLSCGTKIKKIVITGSKVIDDGFLSMVAKKCPNL 341
Cdd:cd09293    2 DPLLFILHKLGQITQSNISQLL-RILHSGLEWLELYMCPISDPPLDQLSNCNKLKKLILPGSKLIDDEGLIALAQSCPNL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 218511918 342 EVLDIRACELISDSGIYQIAKRCTKLTTVNFGRKNKGNLITDSSICILIRNNPNLKTVGLAGCHITDKTLWDLAIRCSDH 421
Cdd:cd09293   81 QVLDLRACENITDSGIVALATNCPKLQTINLGRHRNGHLITDVSLSALGKNCTFLQTVGFAGCDVTDKGVWELASGCSKS 160

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 218511918 422 LQRLSLNNCPHITNQSIPLILHSNLFKNISVLELRFANQITNFKPIIEFKRRQEFRGISILIEVCE 487
Cdd:cd09293  161 LERLSLNNCRNLTDQSIPAILASNYFPNLSVLEFRGCPLITDFSRIILFKLWQPRLNKPILVEWCE 226
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
217-476 4.51e-05

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 46.08  E-value: 4.51e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 218511918 217 LFNCLQVNKLFNQVTTEIISQKFFFSDEMKLHSFLQNLKITQIKSKPSLFVFHKLFHAKQDIIELIKSHMDFSNLQWLEF 296
Cdd:COG4886   41 SLLSLLLLLTLLLSLLLRDLLLSSLLLLLSLLLLLLLSLLLLSLLLLGLTDLGDLTNLTELDLSGNEELSNLTNLESLDL 120

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 218511918 297 YMCpKLLPTPEFLSCGTKIKKIVITGSKVIDdgfLSMVAKKCPNLEVLDIRACELisdSGIYQIAKRCTKLTTVNFGrkn 376
Cdd:COG4886  121 SGN-QLTDLPEELANLTNLKELDLSNNQLTD---LPEPLGNLTNLKSLDLSNNQL---TDLPEELGNLTNLKELDLS--- 190

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 218511918 377 kGNLITDSSICilIRNNPNLKTVGLAGCHITDktlWDLAIRCSDHLQRLSLNNCPhITnqSIPLIlhSNLfKNISVLELR 456
Cdd:COG4886  191 -NNQITDLPEP--LGNLTNLEELDLSGNQLTD---LPEPLANLTNLETLDLSNNQ-LT--DLPEL--GNL-TNLEELDLS 258

                        250       260
                 ....*....|....*....|
gi 218511918 457 FaNQITNFKPIIEFKRRQEF 476
Cdd:COG4886  259 N-NQLTDLPPLANLTNLKTL 277
LRR_CC smart00367
Leucine-rich repeat - CC (cysteine-containing) subfamily;
338-363 1.12e-03

Leucine-rich repeat - CC (cysteine-containing) subfamily;


Pssm-ID: 197685 [Multi-domain]  Cd Length: 26  Bit Score: 36.23  E-value: 1.12e-03
                           10        20
                   ....*....|....*....|....*.
gi 218511918   338 CPNLEVLDIRACELISDSGIYQIAKR 363
Cdd:smart00367   1 CPNLRELDLSGCTNITDEGLQALAKG 26
 
Name Accession Description Interval E-value
AMN1 cd09293
Antagonist of mitotic exit network protein 1; Amn1 has been functionally characterized in ...
 262-487 2.90e-120

Antagonist of mitotic exit network protein 1; Amn1 has been functionally characterized in Saccharomyces cerevisiae as a component of the Antagonist of MEN pathway (AMEN). The AMEN network is activated by MEN (mitotic exit network) via an active Cdc14, and in turn switches off MEN. Amn1 constitutes one of the alternative mechanisms by which MEN may be disrupted. Specifically, Amn1 binds Tem1 (Termination of M-phase, a GTPase that belongs to the RAS superfamily), and disrupts its association with Cdc15, the primary downstream target. Amn1 is a leucine-rich repeat (LRR) protein, with 12 repeats in the S. cerevisiae ortholog. As a negative regulator of the signal transduction pathway MEN, overexpression of AMN1 slows the growth of wild type cells. The function of the vertebrate members of this family has not been determined experimentally, they have fewer LRRs that determine the extent of this model.


Pssm-ID: 187754 [Multi-domain]  Cd Length: 226  Bit Score: 353.17  E-value: 2.90e-120
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 218511918 262 KPSLFVFHKLFHAKQDIIELIKsHMDFSNLQWLEFYMCPKLLPTPEFLSCGTKIKKIVITGSKVIDDGFLSMVAKKCPNL 341
Cdd:cd09293    2 DPLLFILHKLGQITQSNISQLL-RILHSGLEWLELYMCPISDPPLDQLSNCNKLKKLILPGSKLIDDEGLIALAQSCPNL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 218511918 342 EVLDIRACELISDSGIYQIAKRCTKLTTVNFGRKNKGNLITDSSICILIRNNPNLKTVGLAGCHITDKTLWDLAIRCSDH 421
Cdd:cd09293   81 QVLDLRACENITDSGIVALATNCPKLQTINLGRHRNGHLITDVSLSALGKNCTFLQTVGFAGCDVTDKGVWELASGCSKS 160

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 218511918 422 LQRLSLNNCPHITNQSIPLILHSNLFKNISVLELRFANQITNFKPIIEFKRRQEFRGISILIEVCE 487
Cdd:cd09293  161 LERLSLNNCRNLTDQSIPAILASNYFPNLSVLEFRGCPLITDFSRIILFKLWQPRLNKPILVEWCE 226
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
217-476 4.51e-05

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 46.08  E-value: 4.51e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 218511918 217 LFNCLQVNKLFNQVTTEIISQKFFFSDEMKLHSFLQNLKITQIKSKPSLFVFHKLFHAKQDIIELIKSHMDFSNLQWLEF 296
Cdd:COG4886   41 SLLSLLLLLTLLLSLLLRDLLLSSLLLLLSLLLLLLLSLLLLSLLLLGLTDLGDLTNLTELDLSGNEELSNLTNLESLDL 120

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 218511918 297 YMCpKLLPTPEFLSCGTKIKKIVITGSKVIDdgfLSMVAKKCPNLEVLDIRACELisdSGIYQIAKRCTKLTTVNFGrkn 376
Cdd:COG4886  121 SGN-QLTDLPEELANLTNLKELDLSNNQLTD---LPEPLGNLTNLKSLDLSNNQL---TDLPEELGNLTNLKELDLS--- 190

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 218511918 377 kGNLITDSSICilIRNNPNLKTVGLAGCHITDktlWDLAIRCSDHLQRLSLNNCPhITnqSIPLIlhSNLfKNISVLELR 456
Cdd:COG4886  191 -NNQITDLPEP--LGNLTNLEELDLSGNQLTD---LPEPLANLTNLETLDLSNNQ-LT--DLPEL--GNL-TNLEELDLS 258

                        250       260
                 ....*....|....*....|
gi 218511918 457 FaNQITNFKPIIEFKRRQEF 476
Cdd:COG4886  259 N-NQLTDLPPLANLTNLKTL 277
LRR_RI cd00116
Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 ...
 339-428 1.61e-04

Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond to structural units consisting of a beta strand (LxxLxLxxN/CxL conserved pattern) and an alpha helix. This alignment contains 12 strands corresponding to 11 full repeats, consistent with the extent observed in the subfamily acting as Ran GTPase Activating Proteins (RanGAP1).


Pssm-ID: 238064 [Multi-domain]  Cd Length: 319  Bit Score: 43.88  E-value: 1.61e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 218511918 339 PNLEVLDIRACElISDSGIYQIA---KRCTKLTTVNFGRknkgNLITDSSIC----ILIRNNPNLKTVGLAGCHITDKTL 411
Cdd:cd00116  193 CNLEVLDLNNNG-LTDEGASALAetlASLKSLEVLNLGD----NNLTDAGAAalasALLSPNISLLTLSLSCNDITDDGA 267

                         90
                 ....*....|....*....
gi 218511918 412 WDLAIRCSDH--LQRLSLN 428
Cdd:cd00116  268 KDLAEVLAEKesLLELDLR 286
LRR_CC smart00367
Leucine-rich repeat - CC (cysteine-containing) subfamily;
338-363 1.12e-03

Leucine-rich repeat - CC (cysteine-containing) subfamily;


Pssm-ID: 197685 [Multi-domain]  Cd Length: 26  Bit Score: 36.23  E-value: 1.12e-03
                           10        20
                   ....*....|....*....|....*.
gi 218511918   338 CPNLEVLDIRACELISDSGIYQIAKR 363
Cdd:smart00367   1 CPNLRELDLSGCTNITDEGLQALAKG 26
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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