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Conserved domains on  [gi|81883340|sp|Q5RJQ7|]
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RecName: Full=Succinate dehydrogenase assembly factor 2, mitochondrial; Short=SDH assembly factor 2; Short=SDHAF2; Flags: Precursor

Protein Classification

succinate dehydrogenase assembly factor 2( domain architecture ID 10511494)

succinate dehydrogenase (SDH) assembly factor 2 plays an essential role in the assembly of SDH, an enzyme complex that is a component of both the tricarboxylic acid cycle and the mitochondrial electron transport chain

Gene Ontology:  GO:0006121|GO:0065003

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Sdh5 pfam03937
Flavinator of succinate dehydrogenase; This family includes the highly conserved mitochondrial ...
66-141 1.82e-28

Flavinator of succinate dehydrogenase; This family includes the highly conserved mitochondrial and bacterial proteins Sdh5/SDHAF2/SdhE. Both yeast and human Sdh5/SDHAF2 interact with the catalytic subunit of the succinate dehydrogenase (SDH) complex, a component of both the electron transport chain and the tricarboxylic acid cycle. Sdh5 is required for SDH-dependent respiration and for Sdh1 flavination (incorporation of the flavin adenine dinucleotide cofactor). Mutational inactivation of Sdh5 confers tumor susceptibility in humans. Bacterial homologs of Sdh5, termed SdhE, are functionally conserved being required for the flavinylation of SdhA and succinate dehydrogenase activity. Like Sdh5, SdhE interacts with SdhA. Furthermore, SdhE was characterized as a FAD co-factor chaperone that directly binds FAD to facilitate the flavinylation of SdhA. Phylogenetic analysis demonstrates that SdhE/Sdh5 proteins evolved only once in an ancestral alpha-proteobacteria prior to the evolution of the mitochondria and now remain in subsequent descendants including eukaryotic mitochondria and the alpha, beta and gamma proteobacteria. This family was previously annotated in Pfam as being a divergent TPR repeat but structural evidence has indicated this is not true. The E. coli protein, YgfY also acts as the antitoxin to the membrane-bound toxin family Cpta, pfam13166, whose E. coli member YgfX, expressed from the same operon as YgfY.


:

Pssm-ID: 461097  Cd Length: 76  Bit Score: 100.20  E-value: 1.82e-28
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 81883340    66 ARLLYESRKRGMLENCILLSLFAKEYLHNMTEKQLNLYDRLINEPsnDWDIYYWATEAKPAPEIFE-NEVMELLREF 141
Cdd:pfam03937   1 RRLRYRSRRRGMLELDLLLGRFADRHLDTLSEEELDAFERLLDEE--DPDLFDWLTGKRPEPEDLElAALIARIRAF 75
 
Name Accession Description Interval E-value
Sdh5 pfam03937
Flavinator of succinate dehydrogenase; This family includes the highly conserved mitochondrial ...
66-141 1.82e-28

Flavinator of succinate dehydrogenase; This family includes the highly conserved mitochondrial and bacterial proteins Sdh5/SDHAF2/SdhE. Both yeast and human Sdh5/SDHAF2 interact with the catalytic subunit of the succinate dehydrogenase (SDH) complex, a component of both the electron transport chain and the tricarboxylic acid cycle. Sdh5 is required for SDH-dependent respiration and for Sdh1 flavination (incorporation of the flavin adenine dinucleotide cofactor). Mutational inactivation of Sdh5 confers tumor susceptibility in humans. Bacterial homologs of Sdh5, termed SdhE, are functionally conserved being required for the flavinylation of SdhA and succinate dehydrogenase activity. Like Sdh5, SdhE interacts with SdhA. Furthermore, SdhE was characterized as a FAD co-factor chaperone that directly binds FAD to facilitate the flavinylation of SdhA. Phylogenetic analysis demonstrates that SdhE/Sdh5 proteins evolved only once in an ancestral alpha-proteobacteria prior to the evolution of the mitochondria and now remain in subsequent descendants including eukaryotic mitochondria and the alpha, beta and gamma proteobacteria. This family was previously annotated in Pfam as being a divergent TPR repeat but structural evidence has indicated this is not true. The E. coli protein, YgfY also acts as the antitoxin to the membrane-bound toxin family Cpta, pfam13166, whose E. coli member YgfX, expressed from the same operon as YgfY.


Pssm-ID: 461097  Cd Length: 76  Bit Score: 100.20  E-value: 1.82e-28
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 81883340    66 ARLLYESRKRGMLENCILLSLFAKEYLHNMTEKQLNLYDRLINEPsnDWDIYYWATEAKPAPEIFE-NEVMELLREF 141
Cdd:pfam03937   1 RRLRYRSRRRGMLELDLLLGRFADRHLDTLSEEELDAFERLLDEE--DPDLFDWLTGKRPEPEDLElAALIARIRAF 75
SdhE COG2938
Succinate dehydrogenase flavin-adding protein, antitoxin component of the CptAB ...
62-145 8.98e-17

Succinate dehydrogenase flavin-adding protein, antitoxin component of the CptAB toxin-antitoxin module [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 442181  Cd Length: 84  Bit Score: 70.58  E-value: 8.98e-17
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81883340  62 ETKRARLLYESRkRGMLENCILLSLFAKEYLHNMTEKQLNLYDRLINEPsnDWDIYYWATEAKPAPEIFENEVMELLREF 141
Cdd:COG2938   4 EARRKRLRWRCR-RGMLELDLLLGRFADAHLDTLSDAELDAFERLLELE--DPDLFDWLMGRKPPPDEELAALVARIRAF 80

                ....
gi 81883340 142 TKNK 145
Cdd:COG2938  81 HQAR 84
 
Name Accession Description Interval E-value
Sdh5 pfam03937
Flavinator of succinate dehydrogenase; This family includes the highly conserved mitochondrial ...
66-141 1.82e-28

Flavinator of succinate dehydrogenase; This family includes the highly conserved mitochondrial and bacterial proteins Sdh5/SDHAF2/SdhE. Both yeast and human Sdh5/SDHAF2 interact with the catalytic subunit of the succinate dehydrogenase (SDH) complex, a component of both the electron transport chain and the tricarboxylic acid cycle. Sdh5 is required for SDH-dependent respiration and for Sdh1 flavination (incorporation of the flavin adenine dinucleotide cofactor). Mutational inactivation of Sdh5 confers tumor susceptibility in humans. Bacterial homologs of Sdh5, termed SdhE, are functionally conserved being required for the flavinylation of SdhA and succinate dehydrogenase activity. Like Sdh5, SdhE interacts with SdhA. Furthermore, SdhE was characterized as a FAD co-factor chaperone that directly binds FAD to facilitate the flavinylation of SdhA. Phylogenetic analysis demonstrates that SdhE/Sdh5 proteins evolved only once in an ancestral alpha-proteobacteria prior to the evolution of the mitochondria and now remain in subsequent descendants including eukaryotic mitochondria and the alpha, beta and gamma proteobacteria. This family was previously annotated in Pfam as being a divergent TPR repeat but structural evidence has indicated this is not true. The E. coli protein, YgfY also acts as the antitoxin to the membrane-bound toxin family Cpta, pfam13166, whose E. coli member YgfX, expressed from the same operon as YgfY.


Pssm-ID: 461097  Cd Length: 76  Bit Score: 100.20  E-value: 1.82e-28
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 81883340    66 ARLLYESRKRGMLENCILLSLFAKEYLHNMTEKQLNLYDRLINEPsnDWDIYYWATEAKPAPEIFE-NEVMELLREF 141
Cdd:pfam03937   1 RRLRYRSRRRGMLELDLLLGRFADRHLDTLSEEELDAFERLLDEE--DPDLFDWLTGKRPEPEDLElAALIARIRAF 75
SdhE COG2938
Succinate dehydrogenase flavin-adding protein, antitoxin component of the CptAB ...
62-145 8.98e-17

Succinate dehydrogenase flavin-adding protein, antitoxin component of the CptAB toxin-antitoxin module [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 442181  Cd Length: 84  Bit Score: 70.58  E-value: 8.98e-17
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81883340  62 ETKRARLLYESRkRGMLENCILLSLFAKEYLHNMTEKQLNLYDRLINEPsnDWDIYYWATEAKPAPEIFENEVMELLREF 141
Cdd:COG2938   4 EARRKRLRWRCR-RGMLELDLLLGRFADAHLDTLSDAELDAFERLLELE--DPDLFDWLMGRKPPPDEELAALVARIRAF 80

                ....
gi 81883340 142 TKNK 145
Cdd:COG2938  81 HQAR 84
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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