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Conserved domains on  [gi|2450958304|sp|Q27650|]
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RecName: Full=Lysozyme 1; AltName: Full=1,4-beta-N-acetylmuramidase; Flags: Precursor

Protein Classification

glycoside hydrolase family 25 protein( domain architecture ID 10157507)

glycoside hydrolase family 25 protein similar to lysozyme that catalyzes the hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins

CAZY:  GH25
EC:  3.2.1.17
Gene Ontology:  GO:0003796|GO:0009253|GO:0016998|GO:0045087|GO:0007165
PubMed:  1747104|8535779|7624375

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GH25_Lys1-like cd06416
Lys-1 is a lysozyme encoded by the Caenorhabditis elegans lys-1 gene. This gene is one of a ...
 14-210 6.30e-84

Lys-1 is a lysozyme encoded by the Caenorhabditis elegans lys-1 gene. This gene is one of a several lysozyme genes upregulated upon infection by the Gram-negative bacterial pathogen Serratia marcescens. Lys-1 contains a glycosyl hydrolase family 25 (GH25) catalytic domain. This family also includes Lys-5 from Caenorhabditis elegans.


:

Pssm-ID: 119378  Cd Length: 196  Bit Score: 247.24  E-value: 6.30e-84
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2450958304  14 KLGIDVSQPTSTSSFTCLRNKGFTtMVIVRAWKSTGSFDTNAPQTLKNANAAGFSienSDVYYYPCISC-GNMAGQVRTF 92
Cdd:cd06416     1 ILGVDISQPTSVSTFQCLKNNGYS-FAIIRAYRSNGSFDPNSVTNIKNARAAGLS---TDVYFFPCINCcGSAAGQVQTF 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2450958304  93 WQKVGQYSLKVKRVWFDIE---GTWTSSVSTNQNYLMQMMNEARAIGIVHGIYGSKYYWGNLFGSSYKYaYASSTPLWYP 169
Cdd:cd06416    77 LQYLKANGIKYGTVWIDIEqnpCQWSSDVASNCQFLQELVSAAKALGLKVGIYSSQYDWSQIFGSSYTC-NFSSLPLWYA 155

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 2450958304 170 HYDNSPSFSDFSSFGGWTSPSMKQYRGDVSVCSAGVDYNYK 210
Cdd:cd06416   156 HYDNNPNFSDFSPFGGWTKPTMKQYSGTTTVCGVSVDLNVY 196
 
Name Accession Description Interval E-value
GH25_Lys1-like cd06416
Lys-1 is a lysozyme encoded by the Caenorhabditis elegans lys-1 gene. This gene is one of a ...
 14-210 6.30e-84

Lys-1 is a lysozyme encoded by the Caenorhabditis elegans lys-1 gene. This gene is one of a several lysozyme genes upregulated upon infection by the Gram-negative bacterial pathogen Serratia marcescens. Lys-1 contains a glycosyl hydrolase family 25 (GH25) catalytic domain. This family also includes Lys-5 from Caenorhabditis elegans.


Pssm-ID: 119378  Cd Length: 196  Bit Score: 247.24  E-value: 6.30e-84
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2450958304  14 KLGIDVSQPTSTSSFTCLRNKGFTtMVIVRAWKSTGSFDTNAPQTLKNANAAGFSienSDVYYYPCISC-GNMAGQVRTF 92
Cdd:cd06416     1 ILGVDISQPTSVSTFQCLKNNGYS-FAIIRAYRSNGSFDPNSVTNIKNARAAGLS---TDVYFFPCINCcGSAAGQVQTF 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2450958304  93 WQKVGQYSLKVKRVWFDIE---GTWTSSVSTNQNYLMQMMNEARAIGIVHGIYGSKYYWGNLFGSSYKYaYASSTPLWYP 169
Cdd:cd06416    77 LQYLKANGIKYGTVWIDIEqnpCQWSSDVASNCQFLQELVSAAKALGLKVGIYSSQYDWSQIFGSSYTC-NFSSLPLWYA 155

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 2450958304 170 HYDNSPSFSDFSSFGGWTSPSMKQYRGDVSVCSAGVDYNYK 210
Cdd:cd06416   156 HYDNNPNFSDFSPFGGWTKPTMKQYSGTTTVCGVSVDLNVY 196
 
Name Accession Description Interval E-value
GH25_Lys1-like cd06416
Lys-1 is a lysozyme encoded by the Caenorhabditis elegans lys-1 gene. This gene is one of a ...
 14-210 6.30e-84

Lys-1 is a lysozyme encoded by the Caenorhabditis elegans lys-1 gene. This gene is one of a several lysozyme genes upregulated upon infection by the Gram-negative bacterial pathogen Serratia marcescens. Lys-1 contains a glycosyl hydrolase family 25 (GH25) catalytic domain. This family also includes Lys-5 from Caenorhabditis elegans.


Pssm-ID: 119378  Cd Length: 196  Bit Score: 247.24  E-value: 6.30e-84
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2450958304  14 KLGIDVSQPTSTSSFTCLRNKGFTtMVIVRAWKSTGSFDTNAPQTLKNANAAGFSienSDVYYYPCISC-GNMAGQVRTF 92
Cdd:cd06416     1 ILGVDISQPTSVSTFQCLKNNGYS-FAIIRAYRSNGSFDPNSVTNIKNARAAGLS---TDVYFFPCINCcGSAAGQVQTF 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2450958304  93 WQKVGQYSLKVKRVWFDIE---GTWTSSVSTNQNYLMQMMNEARAIGIVHGIYGSKYYWGNLFGSSYKYaYASSTPLWYP 169
Cdd:cd06416    77 LQYLKANGIKYGTVWIDIEqnpCQWSSDVASNCQFLQELVSAAKALGLKVGIYSSQYDWSQIFGSSYTC-NFSSLPLWYA 155

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 2450958304 170 HYDNSPSFSDFSSFGGWTSPSMKQYRGDVSVCSAGVDYNYK 210
Cdd:cd06416   156 HYDNNPNFSDFSPFGGWTKPTMKQYSGTTTVCGVSVDLNVY 196
GH25_muramidase cd00599
Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan ...
 16-209 7.04e-16

Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.


Pssm-ID: 119373 [Multi-domain]  Cd Length: 186  Bit Score: 72.38  E-value: 7.04e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2450958304  16 GIDVSQPTSTSSFTCLRNKGfTTMVIVRAWKSTGSFDTNAPQTLKNANAAGFSIEnsdvYYYPCISCGNMAGQVRTFWQK 95
Cdd:cd00599     2 GIDVSSWQGSIDWNAVKAAG-IDFVFIKATEGTTYVDPKFATNRARARAAGLLVG----AYHFARPCANAEAQADNFVNT 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2450958304  96 VGQYSLKVKrVWFDIEGT-WTSSVSTNQNYLMQMMNEARAI-GIVHGIYGSKYYWGNLFGSSykyaYASSTPLWYPHYDN 173
Cdd:cd00599    77 VPRDPGSLP-LVLDVEDTgGGCSAAALAAWLNAFLNEVEALtGKKPIIYTSPSFWDDYLASS----QLSDYPLWIAHYRG 151

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 2450958304 174 spsfSDFSSFGGWTSPSMKQY--RGDVSVCSAGVDYNY 209
Cdd:cd00599   152 ----EPPPAPGAWRPWTLWQYtsSGRVPGISGPVDLNV 185
GH25_Lyc-like cd06525
Lyc muramidase is an autolytic lysozyme (autolysin) from Clostridium acetobutylicum encoded by ...
 16-209 4.21e-06

Lyc muramidase is an autolytic lysozyme (autolysin) from Clostridium acetobutylicum encoded by the lyc gene. Lyc has a glycosyl hydrolase family 25 (GH25) catalytic domain. Endo-N-acetylmuramidases are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.


Pssm-ID: 119385  Cd Length: 184  Bit Score: 45.36  E-value: 4.21e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2450958304  16 GIDVSQPTSTSSFTCLRNKGfTTMVIVRAWKSTGSFDTNAPQTLKNANAAGFSIEnsdvYYYPCISCGNMAGQVRTFWQK 95
Cdd:cd06525     2 GIDISNWQGNINFNAVKDSG-VEVVYIKATEGTTFVDSYFNENYNGAKAAGLKVG----FYHFLVGTSNPEEQAENFYNT 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2450958304  96 VGQYSLKVKrVWFDIEGTWTSSVSTNQNYLMQMMNEARAI-GIVHGIYGSKYywgnlFGSSYKYAYASSTPLWYPHYDNs 174
Cdd:cd06525    77 IKGKKMDLK-PALDVEVNFGLSKDELNDYVLRFIEEFEKLsGLKVGIYTYTS-----FINNNLDSRLSSYPLWIANYGV- 149

                         170       180       190
                  ....*....|....*....|....*....|....*..
gi 2450958304 175 psfSDFSSFGGWTSPSMKQY--RGDVSVCSAGVDYNY 209
Cdd:cd06525   150 ---SPPSSNGIWNSWVGFQYseTGRVNGVSGSVDLDE 183
GH25_LytC-like cd06414
The LytC lysozyme of Streptococcus pneumoniae is a bacterial cell wall hydrolase that cleaves ...
 14-209 1.93e-03

The LytC lysozyme of Streptococcus pneumoniae is a bacterial cell wall hydrolase that cleaves the beta1-4-glycosydic bond located between the N-acetylmuramoyl-N-glucosaminyl residues of the cell wall polysaccharide chains. LytC is composed of a C-terminal glycosyl hydrolase family 25 (GH25) domain and an N-terminal choline-binding module (CBM) consisting of eleven homologous repeats that specifically recognizes the choline residues of pneumococcal lipoteichoic and teichoic acids. This domain arrangement is the reverse of the major pneumococcal autolysin, LytA, and the CPL-1-like lytic enzymes of the pneumococcal bacteriophages, in which the CBM (consisting of six repeats) is at the C-terminus. This model represents the C-terminal catalytic domain of the LytC-like enzymes.


Pssm-ID: 119376  Cd Length: 191  Bit Score: 37.93  E-value: 1.93e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2450958304  14 KLGIDVSQPTSTSSFTCLRNKGFTtMVIVRA---WKSTGSFDTNAPQTLKNANAAGFSIensDVYYYPCISCGNMAgqvr 90
Cdd:cd06414     1 KKGIDVSEWQGDIDWKKVKASGVD-FAIIRAgygGYGELQEDKYFEENIKGAKAAGIPV---GVYFYSYAVTVAEA---- 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2450958304  91 tfwQKVGQYSLKVKR-------VWFDIEGTWTSSVSTNQNYLMQM----MNEARAIGIVHGIYGSKYYWGNLFGSSYKYA 159
Cdd:cd06414    73 ---REEAEFVLRLIKgyklsypVYYDLEDETQLGAGLSKDQRTDIanafCETIEAAGYYPGIYANLSWLTNKLDDERLSK 149

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2450958304 160 YasstPLWYPHYDNspsfsdfsSFGGWTSPSMKQY--RGDVSVCSAGVDYNY 209
Cdd:cd06414   150 Y----DVWVAQYGN--------SPTYPGNYGMWQYtsSGSVPGISGNVDLNY 189
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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