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Conserved domains on  [gi|1387832852|gb|PWA96139|]
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rmlC-like cupins superfamily protein [Artemisia annua]

Protein Classification

germin family protein( domain architecture ID 14388826)

germin family protein is a cupin domain-containing protein similar to oxalate oxidase (also called germin) that catalyzes the manganese-dependent oxidative decarboxylation of oxalate to carbon dioxide and hydrogen peroxide

CATH:  2.60.120.10
Gene Ontology:  GO:0046872
PubMed:  14697267|19478949
SCOP:  3001825

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
cupin_OxOx cd02241
Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3. ...
 26-213 1.39e-65

Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3.4) catalyzes the manganese-dependent oxidative decarboxylation of oxalate to carbon dioxide and hydrogen peroxide (H2O2). It is widespread in fungi and various plant tissues and may play a role in plant signaling and defense. This enzyme has been employed in a widely used assay for detecting urinary oxalate levels. Also, the gene encoding OxOx from barley roots has been expressed in oilseed rape in order to provide a defense against externally supplied oxalic acid. In germin, the predominant protein produced during the early phase of wheat germination, it is believed that H2O2 production is employed as a defense mechanism in response to infection by pathogens. Germin is also a marker of growth onset in cell walls in germinating cereals. The H2O2 produced by OxOx, together with the Ca2+ released by degradation of calcium oxalate, are thought to mediate cell wall cross-linking at high concentrations. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


:

Pssm-ID: 380368  Cd Length: 191  Bit Score: 200.90  E-value: 1.39e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852  26 DNLQDICPTYTSPDQsmFVNGFPCKNPSNISTSDFKNLLLSQKGETDAFLRSSVTIVTAAEFPGLNTLGLSNARTDLEVD 105
Cdd:cd02241     3 DPLQDFCVADLSSPR--TPNGYPCKDPALVTADDFVFDFLNPPGNTSNPLGGSVTLANVANFPALNGLGISMARGDLAPC 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852 106 GLVMPHTHPRSTEMIFVAKGVVIAGFIDTNS-KLFQSVLREGDVFVFPKGLLHYCLNSGFDDALIYSFFNAQNPGVVDIS 184
Cdd:cd02241    81 GVNPPHTHPRATELLYVVEGTLYVGFVDENGnRLFTKTLNPGDVFVFPQGLIHFQFNPGCEPAVFVAAFNSEDPGTQQIA 160

                         170       180       190
                  ....*....|....*....|....*....|
gi 1387832852 185 NAMFG-GSESEMVRVALakLVSLNRVEDVR 213
Cdd:cd02241   161 QALFGlPPPDDVLAAAF--GLDGAQVEKLK 188
 
Name Accession Description Interval E-value
cupin_OxOx cd02241
Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3. ...
 26-213 1.39e-65

Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3.4) catalyzes the manganese-dependent oxidative decarboxylation of oxalate to carbon dioxide and hydrogen peroxide (H2O2). It is widespread in fungi and various plant tissues and may play a role in plant signaling and defense. This enzyme has been employed in a widely used assay for detecting urinary oxalate levels. Also, the gene encoding OxOx from barley roots has been expressed in oilseed rape in order to provide a defense against externally supplied oxalic acid. In germin, the predominant protein produced during the early phase of wheat germination, it is believed that H2O2 production is employed as a defense mechanism in response to infection by pathogens. Germin is also a marker of growth onset in cell walls in germinating cereals. The H2O2 produced by OxOx, together with the Ca2+ released by degradation of calcium oxalate, are thought to mediate cell wall cross-linking at high concentrations. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380368  Cd Length: 191  Bit Score: 200.90  E-value: 1.39e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852  26 DNLQDICPTYTSPDQsmFVNGFPCKNPSNISTSDFKNLLLSQKGETDAFLRSSVTIVTAAEFPGLNTLGLSNARTDLEVD 105
Cdd:cd02241     3 DPLQDFCVADLSSPR--TPNGYPCKDPALVTADDFVFDFLNPPGNTSNPLGGSVTLANVANFPALNGLGISMARGDLAPC 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852 106 GLVMPHTHPRSTEMIFVAKGVVIAGFIDTNS-KLFQSVLREGDVFVFPKGLLHYCLNSGFDDALIYSFFNAQNPGVVDIS 184
Cdd:cd02241    81 GVNPPHTHPRATELLYVVEGTLYVGFVDENGnRLFTKTLNPGDVFVFPQGLIHFQFNPGCEPAVFVAAFNSEDPGTQQIA 160

                         170       180       190
                  ....*....|....*....|....*....|
gi 1387832852 185 NAMFG-GSESEMVRVALakLVSLNRVEDVR 213
Cdd:cd02241   161 QALFGlPPPDDVLAAAF--GLDGAQVEKLK 188
Cupin_1 pfam00190
Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' ...
 77-189 2.38e-35

Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' is the Latin term for a small barrel). This family contains 11S and 7S plant seed storage proteins, and germins. Plant seed storage proteins provide the major nitrogen source for the developing plant.


Pssm-ID: 395138  Cd Length: 151  Bit Score: 122.44  E-value: 2.38e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852  77 SSVTIVTAAEFPGLNTLGLSNARTDLEVDGLVMPHTHPRSTEMIFVAKGVVIAGFIDTN--SKLFQSVLREGDVFVFPKG 154
Cdd:pfam00190  15 GRVTTVNSKNLPGLNTLGISAARVDLAPGGMNPPHWHPNATEILYVLQGRGRVGFVVPGngNRVFHKVLREGDVFVVPQG 94

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 1387832852 155 LLHYCLNSGFDDALIYSFFNAQNPGVVDISNAMFG 189
Cdd:pfam00190  95 LPHFQYNIGDEPAVAFVAFDTNNPGNQSILAGGFS 129
Cupin_1 smart00835
Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' ...
 78-188 2.21e-30

Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' is the Latin term for a small barrel). This family contains 11S and 7S plant seed storage proteins, and germins. Plant seed storage proteins provide the major nitrogen source for the developing plant.


Pssm-ID: 214845 [Multi-domain]  Cd Length: 146  Bit Score: 109.68  E-value: 2.21e-30
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852   78 SVTIVTAAEFPGLNTLGLSNARTDLEVDGLVMPHTHPRSTEMIFVAKGVVIAGFIDTN-SKLFQSVLREGDVFVFPKGLL 156
Cdd:smart00835  13 RLREADPTNFPALNGLGISAARVNLEPGGMLPPHYHPRATELLYVVRGEGRVGVVDPNgNKVYDARLREGDVFVVPQGHP 92

                           90       100       110
                   ....*....|....*....|....*....|..
gi 1387832852  157 HYCLNSGfDDALIYSFFNAQNPGVVDISNAMF 188
Cdd:smart00835  93 HFQVNSG-DENLEFVAFNTNDPNRRFFLAGRN 123
OxdD COG2140
Oxalate decarboxylase/archaeal phosphoglucose isomerase, cupin superfamily [Carbohydrate ...
 100-187 4.07e-08

Oxalate decarboxylase/archaeal phosphoglucose isomerase, cupin superfamily [Carbohydrate transport and metabolism]; Oxalate decarboxylase/archaeal phosphoglucose isomerase, cupin superfamily is part of the Pathway/BioSystem: Glycolysis


Pssm-ID: 441743 [Multi-domain]  Cd Length: 115  Bit Score: 49.96  E-value: 4.07e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852 100 TDLEVDGLVMPHTHPRSTEMIFVAKGVVIAGFIDTNSKLFQSVLREGDVFVFPKGLLHYCLNSGFDDALIYSFFNA---Q 176
Cdd:COG2140     8 TVLEPGGVREEHWHPNAAEWYYVLSGEARMTVQDPPGRARTVDVGPGDVVYVPPGYGHYIINTGDEPLVFLAVFDDdagS 87

                          90
                  ....*....|.
gi 1387832852 177 NPGVVDISNAM 187
Cdd:COG2140    88 DYGTISLSGWL 98
bicupin_oxalic TIGR03404
bicupin, oxalate decarboxylase family; Members of this protein family are defined as bicupins ...
 78-184 1.24e-03

bicupin, oxalate decarboxylase family; Members of this protein family are defined as bicupins as they have two copies of the cupin domain (pfam00190). Two different known activities for members of this family are oxalate decarboxylase (EC 4.1.1.2) and oxalate oxidase (EC 1.2.3.4), although the latter activity has more often been found in distantly related monocupin (germin) proteins.


Pssm-ID: 274565 [Multi-domain]  Cd Length: 367  Bit Score: 39.23  E-value: 1.24e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852  78 SVTIVTAAEFPGLNTLglSNARTDLEVDGLVMPHTHPRSTEMIFVAKGVVIAGFIDTNSKLFQSVLREGDVFVFPKGLLH 157
Cdd:TIGR03404 230 TVRIADSTNFPVSKTI--AAAIVTVEPGAMRELHWHPNADEWQYFIQGQARMTVFAAGGNARTFDYQAGDVGYVPRNMGH 307

                          90       100
                  ....*....|....*....|....*...
gi 1387832852 158 YCLNSGfDDALIY-SFFNAqnPGVVDIS 184
Cdd:TIGR03404 308 YVENTG-DETLVFlEVFKA--DRFADVS 332
PLN00212 PLN00212
glutelin; Provisional
 131-185 4.37e-03

glutelin; Provisional


Pssm-ID: 215106 [Multi-domain]  Cd Length: 493  Bit Score: 37.49  E-value: 4.37e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1387832852 131 FIDTNSKLFQsvLREGDVFVFPKGLLHYCLNSGfdDALIYSFFnaqnpgVVDISN 185
Cdd:PLN00212  142 FRDEHQKIHQ--FRQGDVVALPAGVAHWFYNDG--DAPVVALY------VYDINN 186
 
Name Accession Description Interval E-value
cupin_OxOx cd02241
Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3. ...
 26-213 1.39e-65

Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3.4) catalyzes the manganese-dependent oxidative decarboxylation of oxalate to carbon dioxide and hydrogen peroxide (H2O2). It is widespread in fungi and various plant tissues and may play a role in plant signaling and defense. This enzyme has been employed in a widely used assay for detecting urinary oxalate levels. Also, the gene encoding OxOx from barley roots has been expressed in oilseed rape in order to provide a defense against externally supplied oxalic acid. In germin, the predominant protein produced during the early phase of wheat germination, it is believed that H2O2 production is employed as a defense mechanism in response to infection by pathogens. Germin is also a marker of growth onset in cell walls in germinating cereals. The H2O2 produced by OxOx, together with the Ca2+ released by degradation of calcium oxalate, are thought to mediate cell wall cross-linking at high concentrations. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380368  Cd Length: 191  Bit Score: 200.90  E-value: 1.39e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852  26 DNLQDICPTYTSPDQsmFVNGFPCKNPSNISTSDFKNLLLSQKGETDAFLRSSVTIVTAAEFPGLNTLGLSNARTDLEVD 105
Cdd:cd02241     3 DPLQDFCVADLSSPR--TPNGYPCKDPALVTADDFVFDFLNPPGNTSNPLGGSVTLANVANFPALNGLGISMARGDLAPC 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852 106 GLVMPHTHPRSTEMIFVAKGVVIAGFIDTNS-KLFQSVLREGDVFVFPKGLLHYCLNSGFDDALIYSFFNAQNPGVVDIS 184
Cdd:cd02241    81 GVNPPHTHPRATELLYVVEGTLYVGFVDENGnRLFTKTLNPGDVFVFPQGLIHFQFNPGCEPAVFVAAFNSEDPGTQQIA 160

                         170       180       190
                  ....*....|....*....|....*....|
gi 1387832852 185 NAMFG-GSESEMVRVALakLVSLNRVEDVR 213
Cdd:cd02241   161 QALFGlPPPDDVLAAAF--GLDGAQVEKLK 188
Cupin_1 pfam00190
Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' ...
 77-189 2.38e-35

Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' is the Latin term for a small barrel). This family contains 11S and 7S plant seed storage proteins, and germins. Plant seed storage proteins provide the major nitrogen source for the developing plant.


Pssm-ID: 395138  Cd Length: 151  Bit Score: 122.44  E-value: 2.38e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852  77 SSVTIVTAAEFPGLNTLGLSNARTDLEVDGLVMPHTHPRSTEMIFVAKGVVIAGFIDTN--SKLFQSVLREGDVFVFPKG 154
Cdd:pfam00190  15 GRVTTVNSKNLPGLNTLGISAARVDLAPGGMNPPHWHPNATEILYVLQGRGRVGFVVPGngNRVFHKVLREGDVFVVPQG 94

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 1387832852 155 LLHYCLNSGFDDALIYSFFNAQNPGVVDISNAMFG 189
Cdd:pfam00190  95 LPHFQYNIGDEPAVAFVAFDTNNPGNQSILAGGFS 129
Cupin_1 smart00835
Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' ...
 78-188 2.21e-30

Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' is the Latin term for a small barrel). This family contains 11S and 7S plant seed storage proteins, and germins. Plant seed storage proteins provide the major nitrogen source for the developing plant.


Pssm-ID: 214845 [Multi-domain]  Cd Length: 146  Bit Score: 109.68  E-value: 2.21e-30
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852   78 SVTIVTAAEFPGLNTLGLSNARTDLEVDGLVMPHTHPRSTEMIFVAKGVVIAGFIDTN-SKLFQSVLREGDVFVFPKGLL 156
Cdd:smart00835  13 RLREADPTNFPALNGLGISAARVNLEPGGMLPPHYHPRATELLYVVRGEGRVGVVDPNgNKVYDARLREGDVFVVPQGHP 92

                           90       100       110
                   ....*....|....*....|....*....|..
gi 1387832852  157 HYCLNSGfDDALIYSFFNAQNPGVVDISNAMF 188
Cdd:smart00835  93 HFQVNSG-DENLEFVAFNTNDPNRRFFLAGRN 123
cupin_OxDC-like cd20306
Oxalate decarboxylase (OxDC)-like cupin domain; This subfamily contains bacterial and ...
 78-186 1.01e-18

Oxalate decarboxylase (OxDC)-like cupin domain; This subfamily contains bacterial and eukaryotic cupin domains of proteins homologous to oxalate decarboxylase (OxDC; EC 4.1.1.2) such as MSMEG_2254, a putative OxDC from Mycobacterium smegmatis. OxDC is a manganese-dependent bicupin that catalyzes the conversion of oxalate to formate and carbon dioxide, utilizing dioxygen as a cofactor. It is evolutionarily related to oxalate oxidase (OxOx or germin; EC 1.2.3.4) which, in contrast, converts oxalate and dioxygen to carbon dioxide and hydrogen peroxide. OxDC is classified as a bicupin because it contains two cupin folds with each domain containing one manganese binding site, with four manganese binding residues (three histidines and one glutamate) conserved as well as a number of hydrophobic residues.


Pssm-ID: 380440 [Multi-domain]  Cd Length: 151  Bit Score: 79.17  E-value: 1.01e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852  78 SVTIVTAAEFPGLNtlGLSNARTDLEVDGLVMPHTHPRSTEMIFVAKGVVIAGFIDTNSKLFQSVLREGDVFVFPKGLLH 157
Cdd:cd20306    19 SIRQATADQLPVLK--GLSIYRLRLSPGGIREPHWHPNANELGYVISGEARVSILDPTGSLDTFTVKPGQVVFIPQGWLH 96

                          90       100
                  ....*....|....*....|....*....
gi 1387832852 158 YCLNSGFDDALIYSFFNAQNPGVVDISNA 186
Cdd:cd20306    97 WIENVGDEEAHLLIFFNHETPEDIGLSDS 125
cupin_OxDC cd02240
Oxalate decarboxylase (OxDC), cupin domain; Oxalate decarboxylase (OxDC; EC 4.1.1.2) is a ...
 78-196 1.36e-14

Oxalate decarboxylase (OxDC), cupin domain; Oxalate decarboxylase (OxDC; EC 4.1.1.2) is a manganese-dependent bicupin that catalyzes the conversion of oxalate to formate and carbon dioxide, utilizing dioxygen as a cofactor. It is evolutionarily related to oxalate oxidase (OxOx or germin; EC 1.2.3.4) which, in contrast, converts oxalate and dioxygen to carbon dioxide and hydrogen peroxide. OxDC is classified as a bicupin because it contains two cupin folds and both domains are included in this alignment. Each OxDC cupin domain contains one manganese binding site, with four manganese binding residues (three histidines and one glutamate) conserved as well as a number of hydrophobic residues. Members of this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold.


Pssm-ID: 380367 [Multi-domain]  Cd Length: 145  Bit Score: 68.27  E-value: 1.36e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852  78 SVTIVTAAEFPGLNtlGLSNARTDLEVDGLVMPHTHPRSTEMIFVAKGVVIAGFIDTNSKLFQSVLREGDVFVFPKGLLH 157
Cdd:cd02240    12 SVRIATVTNFPISK--DLSSALVRVAPGAMRELHWHPNTAEWQYVISGSARVTVFDEDGRFETFNLGAGDVGYVPSGSGH 89

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 1387832852 158 YCLNSGFDDALIYSFFNaqNPGVVDISNAMFGGSESEMV 196
Cdd:cd02240    90 HIENIGDEDAEFLLIFD--DGTFADVSLPWWLAMTPEEV 126
OxdD COG2140
Oxalate decarboxylase/archaeal phosphoglucose isomerase, cupin superfamily [Carbohydrate ...
 100-187 4.07e-08

Oxalate decarboxylase/archaeal phosphoglucose isomerase, cupin superfamily [Carbohydrate transport and metabolism]; Oxalate decarboxylase/archaeal phosphoglucose isomerase, cupin superfamily is part of the Pathway/BioSystem: Glycolysis


Pssm-ID: 441743 [Multi-domain]  Cd Length: 115  Bit Score: 49.96  E-value: 4.07e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852 100 TDLEVDGLVMPHTHPRSTEMIFVAKGVVIAGFIDTNSKLFQSVLREGDVFVFPKGLLHYCLNSGFDDALIYSFFNA---Q 176
Cdd:COG2140     8 TVLEPGGVREEHWHPNAAEWYYVLSGEARMTVQDPPGRARTVDVGPGDVVYVPPGYGHYIINTGDEPLVFLAVFDDdagS 87

                          90
                  ....*....|.
gi 1387832852 177 NPGVVDISNAM 187
Cdd:COG2140    88 DYGTISLSGWL 98
cupin_RmlC-like cd02208
RmlC-like cupin superfamily; This superfamily contains proteins similar to the RmlC (dTDP ...
 99-173 4.77e-08

RmlC-like cupin superfamily; This superfamily contains proteins similar to the RmlC (dTDP (deoxythymidine diphosphates)-4-dehydrorhamnose 3,5-epimerase)-like cupins. RmlC is a dTDP-sugar isomerase involved in the synthesis of L-rhamnose, a saccharide required for the virulence of some pathogenic bacteria. Cupins are a functionally diverse superfamily originally discovered based on the highly conserved motif found in germin and germin-like proteins. This conserved motif forms a beta-barrel fold found in all of the cupins, giving rise to the name cupin ('cupa' is the Latin term for small barrel). The active site of members of this superfamily is generally located at the center of a conserved barrel and usually includes a metal ion. The different functional classes in this superfamily include single domain bacterial isomerases and epimerases involved in the modification of cell wall carbohydrates, two domain bicupins such as the desiccation-tolerant seed storage globulins, and multidomain nuclear transcription factors involved in legume root nodulation.


Pssm-ID: 380338 [Multi-domain]  Cd Length: 73  Bit Score: 48.63  E-value: 4.77e-08
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1387832852  99 RTDLEVDGLVMPHTHPRSTEMIFVAKGvviAGFIDTNSKlFQSVLREGDVFVFPKGLLHYCLNSGFDDALIYSFF 173
Cdd:cd02208     3 VVTLPPGTSSPPHWHPEQDEIFYVLSG---EGELTLDDG-ETVELKAGDIVLIPPGVPHSFVNTSDEPAVFLVVS 73
cupin_OxDC_C cd20305
Oxalate decarboxylase (OxDC), C-terminal cupin domain; This model represents the C-terminal ...
 78-197 8.36e-08

Oxalate decarboxylase (OxDC), C-terminal cupin domain; This model represents the C-terminal cupin domain of oxalate decarboxylase (OxDC; EC 4.1.1.2), a manganese-dependent bicupin that catalyzes the conversion of oxalate to formate and carbon dioxide, utilizing dioxygen as a cofactor. It is evolutionarily related to oxalate oxidase (OxOx or germin; EC 1.2.3.4) which, in contrast, converts oxalate and dioxygen to carbon dioxide and hydrogen peroxide. OxDC is classified as a bicupin because it contains two cupin folds with each domain containing one manganese binding site, with four manganese binding residues (three histidines and one glutamate) conserved as well as a number of hydrophobic residues. Members of this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold.


Pssm-ID: 380439 [Multi-domain]  Cd Length: 153  Bit Score: 49.89  E-value: 8.36e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852  78 SVTIVTAAEFPGLNTLglSNARTDLEVDGLVMPHTHPRSTEMIFVAKG----VVIAGfiDTNSKLFQsvLREGDVFVFPK 153
Cdd:cd20305    19 SVRIVDSKNFPISTTI--AAALVTLEPGALRELHWHPNADEWQYYISGkarmTVFAS--GGRARTFD--FQAGDVGYVPR 92

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 1387832852 154 GLLHYCLNSGFDDALIYSFFNAqnPGVVDIS-NAMFGGSESEMVR 197
Cdd:cd20305    93 GYGHYIENTGDEPLEFLEVFNS--GRYQDISlSQWLALTPPDLVA 135
Cupin_2 pfam07883
Cupin domain; This family represents the conserved barrel domain of the 'cupin' superfamily ( ...
 110-173 3.46e-07

Cupin domain; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' is the Latin term for a small barrel).


Pssm-ID: 462300 [Multi-domain]  Cd Length: 71  Bit Score: 46.10  E-value: 3.46e-07
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1387832852 110 PHTHPRSTEMIFVAKGVVIagfIDTNSKLFqsVLREGDVFVFPKGLLHYCLNSGFDDALIYSFF 173
Cdd:pfam07883  13 PHRHPGEDEFFYVLEGEGE---LTVDGEEV--VLKAGDSVYFPAGVPHRFRNTGDEPARLLDVY 71
ManC COG0662
Mannose-6-phosphate isomerase, cupin superfamily [Carbohydrate transport and metabolism];
94-183 4.13e-06

Mannose-6-phosphate isomerase, cupin superfamily [Carbohydrate transport and metabolism];


Pssm-ID: 440426 [Multi-domain]  Cd Length: 114  Bit Score: 44.36  E-value: 4.13e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852  94 GLSNARTDLEVDGLVMPHTHPRSTEMIFVAKGVviaGFIDTNSKLFqsVLREGDVFVFPKGLLHYCLNSGFDDALIYSFF 173
Cdd:COG0662    26 RLSVKRITVPPGAELSLHVHPHRDEFFYVLEGT---GEVTIGDEEV--ELKAGDSVYIPAGVPHRLRNPGDEPLELLEVQ 100

                          90
                  ....*....|
gi 1387832852 174 NAQNPGVVDI 183
Cdd:COG0662   101 APAYLGEDDI 110
cupin_7S_11S_C cd20285
7S and 11S seed storage globulin, C-terminal cupin domain; This family contains the C-terminal ...
 78-153 6.22e-06

7S and 11S seed storage globulin, C-terminal cupin domain; This family contains the C-terminal cupin domains of 7S and 11S seed storage proteins. The 7S globulins include soybean allergen beta-conglycinin, peanut allergen conarachin (Ara h 1), walnut allergen Jug r 2, and lentil allergen Len c 1. Proteins in this family perform various functions, including a role in sucrose binding, desiccation, defense against microbes and oxidative stress. The 11S globulins include many common food allergens such as the peanut major allergen Ara h 3, almond allergen Pru du 6, pecan allergen Car i 4, hazelnut nut allergen Cor a 9, Brazil nut allergen Ber e 2, cashew allergen Ana o 2, pistachio allergen Pis v 2/5, and walnut allergen Jug n/r 4. These plant seed storage globulins have tandem cupin-like beta-barrel folds (referred to as a bicupin). Storage proteins are the cause of well-known allergic reactions to peanuts and cereals. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold.


Pssm-ID: 380420  Cd Length: 109  Bit Score: 43.75  E-value: 6.22e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1387832852  78 SVTIVTAAEFPGLNTLGLSNARTDLEVDGLVMPHTHPRSTEMIFVAKGVVIAGFIDTN-SKLFQSVLREGDVFVFPK 153
Cdd:cd20285     2 NVTERTSNDFPILKSLNLLASSISLEEGAMFVPHYYSKAIVILVVNEGRAHIQVVGPKgYESYDAELSKGDVFVVPA 78
cupin_MAE_RS03005 cd06987
Microcystis aeruginosa MAE_RS03005 and related proteins, cupin domain; This family includes ...
 103-163 6.57e-06

Microcystis aeruginosa MAE_RS03005 and related proteins, cupin domain; This family includes bacterial and some eukaryotic proteins homologous to MAE_RS03005, a Microcystis aeruginosa protein of unknown function. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380392 [Multi-domain]  Cd Length: 122  Bit Score: 43.79  E-value: 6.57e-06
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1387832852 103 EVDGLVMPHTHPRSTEMIFVAKGVVIAgFIDTnsklfQSV-LREGDVFVFPKGLLHYCLNSG 163
Cdd:cd06987    36 DPGGRTPPNTHPAAHEMFFVLAGEGRA-YCDG-----QRVpLRPGDALVVPPGSEHVIENTG 91
cupin_YdbB-like cd02226
Bacillus subtilis YdbB and related proteins, cupin domain; This family includes bacterial ...
 111-157 5.60e-05

Bacillus subtilis YdbB and related proteins, cupin domain; This family includes bacterial proteins homologous to YdbB, a Bacillus subtilis protein of unknown function. It also includes protein Nmb1881 From Neisseria meningitidis, also of unknown function. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380355 [Multi-domain]  Cd Length: 94  Bit Score: 40.50  E-value: 5.60e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*..
gi 1387832852 111 HTHPRSTEMIFVAKGVViagFIDTNSKLFqsVLREGDVFVFPKGLLH 157
Cdd:cd02226    38 HKHDDEDELFLVLEGEL---TIDFRDRDV--TLGPGEFFVVPKGVEH 79
QdoI COG1917
Cupin domain protein related to quercetin dioxygenase [General function prediction only];
110-163 7.86e-05

Cupin domain protein related to quercetin dioxygenase [General function prediction only];


Pssm-ID: 441521 [Multi-domain]  Cd Length: 99  Bit Score: 40.60  E-value: 7.86e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1387832852 110 PHTHPRsTEMIFVAKGVVIagfIDTNSKLFqsVLREGDVFVFPKGLLHYCLNSG 163
Cdd:COG1917    38 WHSHPG-EELIYVLEGEGE---VEVGGEEY--ELKPGDVVFIPPGVPHAFRNLG 85
cupin_11S_legumin_C cd02243
11S legumin seed storage globulin, C-terminal cupin domain; This family contains the ...
 78-153 1.95e-04

11S legumin seed storage globulin, C-terminal cupin domain; This family contains the C-terminal domains of 11S legumin seed storage proteins that supply nutrition for seed germination, such as glycinin and legumin, including many common food allergens such as the peanut major allergen Ara h 3, almond allergen Pru du 6, Pecan allergen Car i 4, hazelnut nut allergen Cor a 9, Brazil nut allergen Ber e 2, cashew allergen Ana o 2, pistachio allergen Pis v 2/5, and walnut allergen Jug n/r 4. These plant seed storage globulins have tandem cupin-like beta-barrel folds (referred to as a bicupin). They are synthesized as propeptides in the endoplasmic reticulum and transported to the secretory vesicles as a homotrimer. The propeptides are processed as they are sorted in the secretory vesicles. The homotrimer binds another homotrimer to form a homohexamer with 32-point symmetry formed by a face-to-face stacking of the two trimers. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold.


Pssm-ID: 380370  Cd Length: 155  Bit Score: 40.54  E-value: 1.95e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852  78 SVTIVTAAEFPGLNTLGLSNARTDLEVDGLVMPHTHPRSTEMIFVAKG---VVIAGfidTNSK-LFQSVLREGDVFVFPK 153
Cdd:cd02243     9 RITTLNSFKLPILRFVGLSAERVKLEPNAMFAPHWNANAHQVIYVTRGsgrVQVVG---DNGKrVLDGEVREGQLLVVPQ 85
cupin_11S_legumin_N cd02242
11S legumin seed storage globulin, N-terminal cupin domain; This family contains the ...
 94-177 1.12e-03

11S legumin seed storage globulin, N-terminal cupin domain; This family contains the N-terminal domains of 11S legumin seed storage proteins that supply nutrition for seed germination, such as glycinin and legumin, including many common food allergens such as the peanut major allergen Ara h 3, almond allergen Pru du 6, Pecan allergen Car i 4, hazelnut nut allergen Cor a 9, Brazil nut allergen Ber e 2, cashew allergen Ana o 2, pistachio allergen Pis v 2/5, and walnut allergen Jug n/r 4. These plant seed storage globulins have tandem cupin-like beta-barrel folds (referred to as a bicupin). They are synthesized as propeptides in the endoplasmic reticulum and transported to the secretory vesicles as a homotrimer. The propeptides are processed as they are sorted in the secretory vesicles. The homotrimer binds another homotrimer to form a homohexamer with 32-point symmetry formed by a face-to-face stacking of the two trimers. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold.


Pssm-ID: 380369  Cd Length: 209  Bit Score: 38.72  E-value: 1.12e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852  94 GLSNARTDLEVDGLVMPHTHPrSTEMIFVAKGVVIAGFIDTNS-KLFQSV-------------------LREGDVFVFPK 153
Cdd:cd02242    34 GVAAGRLTIEPRGLLLPSYSN-APKLAYVLQGRGIVGVVFPGCpETFQSSqqsqgqgqrfrdqhqkvrrIRKGDVIAVPA 112

                          90       100
                  ....*....|....*....|....*..
gi 1387832852 154 GLLHYCLNSGFDDALIYSFF---NAQN 177
Cdd:cd02242   113 GVVHWWYNDGDSDLVIVFLGdtsNNAN 139
bicupin_oxalic TIGR03404
bicupin, oxalate decarboxylase family; Members of this protein family are defined as bicupins ...
 78-184 1.24e-03

bicupin, oxalate decarboxylase family; Members of this protein family are defined as bicupins as they have two copies of the cupin domain (pfam00190). Two different known activities for members of this family are oxalate decarboxylase (EC 4.1.1.2) and oxalate oxidase (EC 1.2.3.4), although the latter activity has more often been found in distantly related monocupin (germin) proteins.


Pssm-ID: 274565 [Multi-domain]  Cd Length: 367  Bit Score: 39.23  E-value: 1.24e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1387832852  78 SVTIVTAAEFPGLNTLglSNARTDLEVDGLVMPHTHPRSTEMIFVAKGVVIAGFIDTNSKLFQSVLREGDVFVFPKGLLH 157
Cdd:TIGR03404 230 TVRIADSTNFPVSKTI--AAAIVTVEPGAMRELHWHPNADEWQYFIQGQARMTVFAAGGNARTFDYQAGDVGYVPRNMGH 307

                          90       100
                  ....*....|....*....|....*...
gi 1387832852 158 YCLNSGfDDALIY-SFFNAqnPGVVDIS 184
Cdd:TIGR03404 308 YVENTG-DETLVFlEVFKA--DRFADVS 332
COG3837 COG3837
Uncharacterized conserved protein, cupin superfamily [Function unknown];
109-170 1.76e-03

Uncharacterized conserved protein, cupin superfamily [Function unknown];


Pssm-ID: 443048 [Multi-domain]  Cd Length: 115  Bit Score: 36.92  E-value: 1.76e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1387832852 109 MPHTHPRSTEMIFVAKGVVIagfIDTNSKLFqsVLREGDVFVFPKGLLHYCLNSGfDDALIY 170
Cdd:COG3837    43 PYHAHSAEEEFVYVLEGELT---LRIGGEEY--VLEPGDSVGFPAGVPHRLRNRG-DEPARY 98
cupin_PA3510-like cd02225
Pseudomonas aeruginosa PA3510 and related proteins, cupin domain; This family includes ...
 110-182 2.09e-03

Pseudomonas aeruginosa PA3510 and related proteins, cupin domain; This family includes bacterial proteins homologous to PA3510, a Pseudomonas aeruginosa protein of unknown function with a beta-barrel fold that belongs to the cupin superfamily.


Pssm-ID: 380354  Cd Length: 150  Bit Score: 37.26  E-value: 2.09e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1387832852 110 PHTHPrSTEMIFVAKGVVIAgFIDTNSKLFQSVLREGDVFVFPKGLLHYCLNSGFDDALIYSFFNAQNPGVVD 182
Cdd:cd02225    68 LHTHE-VEEVFFVLQGRLTV-FWEDEGEEHERELGPRDMISVPAGVYRGFKNIGEEDALMQVMLGTGKPGRPT 138
cupin_7S_vicilin-like_N cd02244
7S vicilin seed storage globulin, N-terminal cupin domain; This family contains the N-terminal ...
 107-179 2.42e-03

7S vicilin seed storage globulin, N-terminal cupin domain; This family contains the N-terminal domains of plant 7S seed storage proteins such as vicilin, and includes beta-conglycinin, phaseolin, canavalin, conglutin-beta, a chromatin protein in Pisum sativum called P54, and a sucrose binding protein in soybean called SBP. These 7S globulins also include soybean allergen beta-conglycinin, peanut allergen conarachin (Ara h 1), walnut allergen Jug r 2, and lentil allergen Len c 1. Proteins in this family perform various functions, including a role in sucrose binding, desiccation, defense against microbes and oxidative stress. The vicilin peptides formed by trypsin or chymotrypsin digestion exhibit antihypertensive effects. These plant seed storage globulins have tandem cupin-like beta-barrel folds (referred to as a bicupin). Storage proteins are the cause of well-known allergic reactions to peanuts and cereals. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold.


Pssm-ID: 380371  Cd Length: 178  Bit Score: 37.49  E-value: 2.42e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1387832852 107 LVMP-HTHprsTEMIF-VAKGVVIAGFIDtNSKLFQSVLREGDVFVFPKGLLHYCLNSGFDDAL-IYSFF---NAQNPG 179
Cdd:cd02244    40 LFLPhHLD---ADMVFyVHTGRGTITWVD-EDKRESYNLERGDVYRIPAGSTFYLVNTDENEKLrIIALFdpvNSLTPG 114
cupin_DRT102 cd06989
Arabidopsis thaliana DRT102 and related proteins, cupin domain; This family includes bacterial ...
 108-158 2.47e-03

Arabidopsis thaliana DRT102 and related proteins, cupin domain; This family includes bacterial and eukaryotic proteins homologous to DNA-damage-repair/toleration protein DRT102 found in Arabidopsis thaliana. DRT102 may be involved in DNA repair from UV damage. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380394  Cd Length: 97  Bit Score: 35.97  E-value: 2.47e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1387832852 108 VMPHTHPrSTEMIFVAKGVViagFIDTNSKLFQSVLRE---GDVFVFPKGLLHY 158
Cdd:cd06989    31 IPPHTHP-DDERVTVISGTF---YLGMGDKFDEAKAKAlpaGSFFTLPAGTPHF 80
PLN00212 PLN00212
glutelin; Provisional
 131-185 4.37e-03

glutelin; Provisional


Pssm-ID: 215106 [Multi-domain]  Cd Length: 493  Bit Score: 37.49  E-value: 4.37e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1387832852 131 FIDTNSKLFQsvLREGDVFVFPKGLLHYCLNSGfdDALIYSFFnaqnpgVVDISN 185
Cdd:PLN00212  142 FRDEHQKIHQ--FRQGDVVALPAGVAHWFYNDG--DAPVVALY------VYDINN 186
cupin_Bh2720-like cd02223
Bacillus halodurans Bh2720 and related proteins, cupin domain; This family includes bacterial, ...
 112-173 8.47e-03

Bacillus halodurans Bh2720 and related proteins, cupin domain; This family includes bacterial, archaeal, and eukaryotic proteins similar to Bh2720, a Bacillus halodurans protein of unknown function with a cupin beta-barrel fold.


Pssm-ID: 380352 [Multi-domain]  Cd Length: 98  Bit Score: 34.83  E-value: 8.47e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1387832852 112 THPRSTEMIFVAKG---VVIAGFidtnsklfQSVLREGDVFVFPKGLLHYCLNSGFDDALIYSFF 173
Cdd:cd02223    28 VHDDVDQFLRIEEGegkAIMGGF--------ESEVKDGDAIIVPAGTWHNVINTGNEPLKLYTIY 84
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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