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Conserved domains on  [gi|548475|sp|P35375|]
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RecName: Full=Prostaglandin E2 receptor EP1 subtype; Short=PGE receptor EP1 subtype; Short=PGE2 receptor EP1 subtype; AltName: Full=Prostanoid EP1 receptor

Protein Classification

G protein-coupled receptor family protein( domain architecture ID 705710)

G protein-coupled receptor family protein is a seven-transmembrane G protein-coupled receptor (7TM-GPCR) family protein which typically transmits an extracellular signal into the cell by the conformational rearrangement of the 7TM helices and by the subsequent binding and activation of an intracellular heterotrimeric G protein; GPCR ligands include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
7tm_GPCRs super family cl28897
seven-transmembrane G protein-coupled receptor superfamily; This hierarchical evolutionary ...
35-360 4.56e-107

seven-transmembrane G protein-coupled receptor superfamily; This hierarchical evolutionary model represents the seven-transmembrane (7TM) receptors, often referred to as G protein-coupled receptors (GPCRs), which transmit physiological signals from the outside of the cell to the inside via G proteins. GPCRs constitute the largest known superfamily of transmembrane receptors across the three kingdoms of life that respond to a wide variety of extracellular stimuli including peptides, lipids, neurotransmitters, amino acids, hormones, and sensory stimuli such as light, smell and taste. All GPCRs share a common structural architecture comprising of seven-transmembrane (TM) alpha-helices interconnected by three extracellular and three intracellular loops. A general feature of GPCR signaling is agonist-induced conformational changes in the receptors, leading to activation of the heterotrimeric G proteins, which consist of the guanine nucleotide-binding G-alpha subunit and the dimeric G-beta-gamma subunits. The activated G proteins then bind to and activate numerous downstream effector proteins, which generate second messengers that mediate a broad range of cellular and physiological processes. However, some 7TM receptors, such as the type 1 microbial rhodopsins, do not activate G proteins. Based on sequence similarity, GPCRs can be divided into six major classes: class A (the rhodopsin-like family), class B (the Methuselah-like, adhesion and secretin-like receptor family), class C (the metabotropic glutamate receptor family), class D (the fungal mating pheromone receptors), class E (the cAMP receptor family), and class F (the frizzled/smoothened receptor family). Nearly 800 human GPCR genes have been identified and are involved essentially in all major physiological processes. Approximately 40% of clinically marketed drugs mediate their effects through modulation of GPCR function for the treatment of a variety of human diseases including bacterial infections.


The actual alignment was detected with superfamily member cd15144:

Pssm-ID: 475119 [Multi-domain]  Cd Length: 294  Bit Score: 317.10  E-value: 4.56e-107
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475    35 GTSPALPIFSMTLGAVSNVLALALLAQVAGRMRRRRSAaTFLLFVASLLAIDLAGHVIPGALVLRLYTAGRAPAGG---- 110
Cdd:cd15144   1 PASPAMPIFSMTLGALSNIVALVILAQSYARFRRRSKA-TFLLFASSLVLTDLAGHVIPGALVLRLYLSGQMPAEEprga 79
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   111 ACHFLGGCMVFFGLCPLLLGCGMAVERCVGVTQPLIHAARVSVARARLALAVLAAMALAVALLPLVHVGRYELQYPGTWC 190
Cdd:cd15144  80 LCQFFGACMVFFGLCPLFLGCAMAVERCVGVTRPLLHSSVVTTTRTKLSLLAIWAVALAVALLPLFRFGRYKPQFPGTWC 159
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   191 FISLGPRGGWRQALLAGLFAGLGLAALLAALVCNTLSGLALLrarwrrrrsrrfrktagpddRRRWGSRGPrlasassas 270
Cdd:cd15144 160 FIKVQPPGSWADVAFALLFSLLGLASLLVSLVCNTISGLTLV--------------------RARLRKKCS--------- 210
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   271 sitsatatlrssrggGSARRVHAHDVEMVGQLVGIMVVSCICWSPLLVLVVLAIGGW---NSNSLQRPLFLAVRLASWNQ 347
Cdd:cd15144 211 ---------------CSNRRAKSHDIEMVVQLVGIMVVSCICWSPLLIFVLISVSRSyerPKHQYEKLLFLGVRLASWNQ 275
                       330
                ....*....|...
gi 548475   348 ILDPWVYILLRQA 360
Cdd:cd15144 276 ILDPWVYILLRRA 288
 
Name Accession Description Interval E-value
7tmA_PGE2_EP1 cd15144
prostaglandin E2 receptor EP1 subtype, member of the class A family of seven-transmembrane G ...
35-360 4.56e-107

prostaglandin E2 receptor EP1 subtype, member of the class A family of seven-transmembrane G protein-coupled receptors; Prostaglandin E2 receptor EP1, also called prostanoid EP1 receptor, is one of four receptor subtypes whose endogenous physiological ligand is prostaglandin E2 (PGE2). Each of these subtypes (EP1-EP4) have unique but overlapping tissue distributions that activate different intracellular signaling pathways. It has been shown that stimulation of the EP1 receptor by PGE2 causes smooth muscle contraction and increased intracellular Ca2+ levels; however, it is still unclear whether EP1 receptor is exclusively coupled to G(q/11), which leading to activation of phospholipase C and phosphatidylinositol hydrolysis. Prostanoids are the cyclooxygenase (COX) metabolites of arachidonic acid, which include the prostaglandins (PGD2, PGE2, PGF2alpha), prostacyclin (PGI2), and thromboxane A2 (TxA2). These five major bioactive prostanoids acts as mediators or modulators in a wide range of physiological and pathophysiological processes within the kidney and play important roles in inflammation, platelet aggregation, and vasoconstriction/relaxation, among many others. They act locally by preferentially interacting with G protein-coupled receptors designated DP, EP. FP, IP, and TP, respectively. The phylogenetic tree suggests that the prostanoid receptors can be grouped into two major branches: G(s)-coupled (DP1, EP2, EP4, and IP) and G(i)- (EP3) or G(q)-coupled (EP1, FP, and TP), forming three clusters.


Pssm-ID: 320272 [Multi-domain]  Cd Length: 294  Bit Score: 317.10  E-value: 4.56e-107
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475    35 GTSPALPIFSMTLGAVSNVLALALLAQVAGRMRRRRSAaTFLLFVASLLAIDLAGHVIPGALVLRLYTAGRAPAGG---- 110
Cdd:cd15144   1 PASPAMPIFSMTLGALSNIVALVILAQSYARFRRRSKA-TFLLFASSLVLTDLAGHVIPGALVLRLYLSGQMPAEEprga 79
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   111 ACHFLGGCMVFFGLCPLLLGCGMAVERCVGVTQPLIHAARVSVARARLALAVLAAMALAVALLPLVHVGRYELQYPGTWC 190
Cdd:cd15144  80 LCQFFGACMVFFGLCPLFLGCAMAVERCVGVTRPLLHSSVVTTTRTKLSLLAIWAVALAVALLPLFRFGRYKPQFPGTWC 159
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   191 FISLGPRGGWRQALLAGLFAGLGLAALLAALVCNTLSGLALLrarwrrrrsrrfrktagpddRRRWGSRGPrlasassas 270
Cdd:cd15144 160 FIKVQPPGSWADVAFALLFSLLGLASLLVSLVCNTISGLTLV--------------------RARLRKKCS--------- 210
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   271 sitsatatlrssrggGSARRVHAHDVEMVGQLVGIMVVSCICWSPLLVLVVLAIGGW---NSNSLQRPLFLAVRLASWNQ 347
Cdd:cd15144 211 ---------------CSNRRAKSHDIEMVVQLVGIMVVSCICWSPLLIFVLISVSRSyerPKHQYEKLLFLGVRLASWNQ 275
                       330
                ....*....|...
gi 548475   348 ILDPWVYILLRQA 360
Cdd:cd15144 276 ILDPWVYILLRRA 288
7tm_1 pfam00001
7 transmembrane receptor (rhodopsin family); This family contains, amongst other ...
280-354 6.47e-04

7 transmembrane receptor (rhodopsin family); This family contains, amongst other G-protein-coupled receptors (GCPRs), members of the opsin family, which have been considered to be typical members of the rhodopsin superfamily. They share several motifs, mainly the seven transmembrane helices, GCPRs of the rhodopsin superfamily. All opsins bind a chromophore, such as 11-cis-retinal. The function of most opsins other than the photoisomerases is split into two steps: light absorption and G-protein activation. Photoisomerases, on the other hand, are not coupled to G-proteins - they are thought to generate and supply the chromophore that is used by visual opsins.


Pssm-ID: 459624 [Multi-domain]  Cd Length: 256  Bit Score: 41.13  E-value: 6.47e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 548475     280 RSSRGGGSARRVhahdVEMVGQLVGIMVVsciCWSPLLVLVVLAIGGWN---SNSLQRPLFLAVRLASWNQILDPWVY 354
Cdd:pfam00001 186 KSSERTQRRRKA----LKTLAVVVVVFIL---CWLPYHIVNLLDSLALDcelSRLLDKALSVTLWLAYVNSCLNPIIY 256
 
Name Accession Description Interval E-value
7tmA_PGE2_EP1 cd15144
prostaglandin E2 receptor EP1 subtype, member of the class A family of seven-transmembrane G ...
35-360 4.56e-107

prostaglandin E2 receptor EP1 subtype, member of the class A family of seven-transmembrane G protein-coupled receptors; Prostaglandin E2 receptor EP1, also called prostanoid EP1 receptor, is one of four receptor subtypes whose endogenous physiological ligand is prostaglandin E2 (PGE2). Each of these subtypes (EP1-EP4) have unique but overlapping tissue distributions that activate different intracellular signaling pathways. It has been shown that stimulation of the EP1 receptor by PGE2 causes smooth muscle contraction and increased intracellular Ca2+ levels; however, it is still unclear whether EP1 receptor is exclusively coupled to G(q/11), which leading to activation of phospholipase C and phosphatidylinositol hydrolysis. Prostanoids are the cyclooxygenase (COX) metabolites of arachidonic acid, which include the prostaglandins (PGD2, PGE2, PGF2alpha), prostacyclin (PGI2), and thromboxane A2 (TxA2). These five major bioactive prostanoids acts as mediators or modulators in a wide range of physiological and pathophysiological processes within the kidney and play important roles in inflammation, platelet aggregation, and vasoconstriction/relaxation, among many others. They act locally by preferentially interacting with G protein-coupled receptors designated DP, EP. FP, IP, and TP, respectively. The phylogenetic tree suggests that the prostanoid receptors can be grouped into two major branches: G(s)-coupled (DP1, EP2, EP4, and IP) and G(i)- (EP3) or G(q)-coupled (EP1, FP, and TP), forming three clusters.


Pssm-ID: 320272 [Multi-domain]  Cd Length: 294  Bit Score: 317.10  E-value: 4.56e-107
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475    35 GTSPALPIFSMTLGAVSNVLALALLAQVAGRMRRRRSAaTFLLFVASLLAIDLAGHVIPGALVLRLYTAGRAPAGG---- 110
Cdd:cd15144   1 PASPAMPIFSMTLGALSNIVALVILAQSYARFRRRSKA-TFLLFASSLVLTDLAGHVIPGALVLRLYLSGQMPAEEprga 79
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   111 ACHFLGGCMVFFGLCPLLLGCGMAVERCVGVTQPLIHAARVSVARARLALAVLAAMALAVALLPLVHVGRYELQYPGTWC 190
Cdd:cd15144  80 LCQFFGACMVFFGLCPLFLGCAMAVERCVGVTRPLLHSSVVTTTRTKLSLLAIWAVALAVALLPLFRFGRYKPQFPGTWC 159
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   191 FISLGPRGGWRQALLAGLFAGLGLAALLAALVCNTLSGLALLrarwrrrrsrrfrktagpddRRRWGSRGPrlasassas 270
Cdd:cd15144 160 FIKVQPPGSWADVAFALLFSLLGLASLLVSLVCNTISGLTLV--------------------RARLRKKCS--------- 210
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   271 sitsatatlrssrggGSARRVHAHDVEMVGQLVGIMVVSCICWSPLLVLVVLAIGGW---NSNSLQRPLFLAVRLASWNQ 347
Cdd:cd15144 211 ---------------CSNRRAKSHDIEMVVQLVGIMVVSCICWSPLLIFVLISVSRSyerPKHQYEKLLFLGVRLASWNQ 275
                       330
                ....*....|...
gi 548475   348 ILDPWVYILLRQA 360
Cdd:cd15144 276 ILDPWVYILLRRA 288
7tmA_Prostanoid_R cd14981
G protein-coupled receptors for prostanoids, member of the class A family of ...
37-361 3.99e-66

G protein-coupled receptors for prostanoids, member of the class A family of seven-transmembrane G protein-coupled receptors; Prostanoids are the cyclooxygenase (COX) metabolites of arachidonic acid, which include the prostaglandins (PGD2, PGE2, PGF2alpha), prostacyclin (PGI2), and thromboxane A2 (TxA2). These five major bioactive prostanoids acts as mediators or modulators in a wide range of physiological and pathophysiological processes within the kidney and play important roles in inflammation, platelet aggregation, and vasoconstriction/relaxation, among many others. They act locally by preferentially interacting with G protein-coupled receptors designated DP, EP. FP, IP, and TP, respectively. The phylogenetic tree suggests that the prostanoid receptors can be grouped into two major branches: G(s)-coupled (DP1, EP2, EP4, and IP) and G(i)- (EP3) or G(q)-coupled (EP1, FP, and TP), forming three clusters.


Pssm-ID: 320112 [Multi-domain]  Cd Length: 288  Bit Score: 212.11  E-value: 3.99e-66
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475    37 SPALPIFSMTLGAVSNVLALALLAqvagRMRRRRSAATFLLFVASLLAIDLAGHVIPGALVLRLYTAGRAPAGG--ACHF 114
Cdd:cd14981   3 SPAPPALMFVFGVLGNLLALIVLA----RSSKSHKWSVFYRLVAGLAITDLLGILLTSPVVLAVYASNFEWDGGqpLCDY 78
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   115 LGGCMVFFGLCPLLLGCGMAVERCVGVTQPLIHAARVSVARARLALAVLAAMALAVALLPLVHVGRYELQYPGTWCFISL 194
Cdd:cd14981  79 FGFMMSFFGLSSLLIVCAMAVERFLAITHPFFYNSHVKKRRARLMLGAVWAFALLIASLPLLGLGSYVLQYPGTWCFLDF 158
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   195 GPrGGWRQALLAGLFAGLGLAALLAALVCNTLSGLALLrarwrrrrsrrfrktagpddRRRWGSRgprlasassassits 274
Cdd:cd14981 159 YS-KNTGDAAYAYLYSILGLLILLVTLLCNLLVIITLL--------------------RMRRRKK--------------- 202
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   275 atatlRSSRGGGSARRVHAHDVEMVGQLVGIMVVSCICWSPLLVLVVLAIGGWnSNSLQRPLFLAVRLASWNQILDPWVY 354
Cdd:cd14981 203 -----RHRRSRRSARRQKRNEIQMVVLLLAITVVFSVCWLPLMIRVLINATGD-SEKNGKTDLLAVRMASWNQILDPWVY 276

                ....*..
gi 548475   355 ILLRQAM 361
Cdd:cd14981 277 ILLRKEV 283
7tmA_FP cd15145
prostaglandin F2-alpha receptor, member of the class A family of seven-transmembrane G ...
42-361 2.10e-48

prostaglandin F2-alpha receptor, member of the class A family of seven-transmembrane G protein-coupled receptors; The PGF2-alpha receptor, also called prostanoid FP receptor, is a class A G-protein coupled receptor whose endogenous ligand is prostaglandin F2-alpha. PGF2-alpha binding to this receptor is coupled to the stimulation of phospholipase C (PLC) pathway via G-protein subunit G(q). This leads to the release of inositol trisphosphate (IP3) and diacylglycerol (DAG) which results in increased intracellular Ca2+ levels and activation of PKC. The receptor activation primarily induces uterine contraction and bronchoconstriction, and stimulates luteolysis. Like most prostanoid receptors, the PGF2-alpha receptor has also been implicated in tumor angiogenesis and metastasis.


Pssm-ID: 320273 [Multi-domain]  Cd Length: 290  Bit Score: 166.15  E-value: 2.10e-48
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475    42 IFSMTLGAVSNVLALALLAQVAGRMRRRrSAATFLLFVASLLAIDLAGHVIPGALVLRLYTAGRA-----PAGGACHFLG 116
Cdd:cd15145   8 IIFMTVGILSNSLAIAILMKAYQRFRQK-SKASFLLLASGLVITDFFGHLINGTIAVFVYASDKDwirfdQSNILCSVFG 86
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   117 GCMVFFGLCPLLLGCGMAVERCVGVTQPLIHAARVSVARARLALAVLAAMALAVALLPLVHVGRYELQYPGTWCFISLGP 196
Cdd:cd15145  87 ICMVFFGLCPLLLGSVMAVERCIGVTKPIFHSTKMTSKHVKMMLSGVCLFAVLVALLPILGHRDYQIQASRTWCFYKTEH 166
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   197 RGGWRQALLAGLFAGLGLAALLAALVCNTLSGLALlrarwrrrrsrrfrktagpddrrrwgsrgprlasassassitsat 276
Cdd:cd15145 167 IEDWEDRFYLLLFSFLGLLALAISFLCNAITGITL--------------------------------------------- 201
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   277 atLRSSRGGGSARRVHAHDVEMVGQLVGIMVVSCICWSPLLV-LVVLAIGGWNSNSLQRPLFLAVRLASWNQILDPWVYI 355
Cdd:cd15145 202 --LRVKFRSQQHRQGRSHHFEMVIQLLAIMCVSCICWSPFLVtMANIGINGRDSLESCETILFALRMATWNQILDPWVYI 279

                ....*.
gi 548475   356 LLRQAM 361
Cdd:cd15145 280 LLRKAV 285
7tmA_TXA2_R cd15143
thromboxane A2 receptor, member of the class A family of seven-transmembrane G protein-coupled ...
65-361 9.87e-36

thromboxane A2 receptor, member of the class A family of seven-transmembrane G protein-coupled receptors; The thromboxane receptor, also known as the prostanoid TP receptor, is a class A G-protein coupled receptor whose endogenous ligand is thromboxane A2 (TXA2). TXA2 is the major product of cyclooxygenase metabolite of arachidonic acid that found predominantly in platelets and stimulates platelet aggregation, Ca2+ influx into platelets, and also causes vasoconstriction. TXA2 has been shown to be involved in immune regulation, angiogenesis and metastasis, among many others. Activation of TXA2 receptor is coupled to G(q) and G(13), resulting in the activations of phospholipase C and RhoGEF, respectively. TXA2 receptor is widely distributed in the body and is abundantly expressed in thymus and spleen.


Pssm-ID: 320271 [Multi-domain]  Cd Length: 296  Bit Score: 133.02  E-value: 9.87e-36
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475    65 RMRRRRSAATFLLFVASLLAIDLAGHVIPGALVLRLYTAGRA-----PAGGACHFLGGCMVFFGLCPLLLGCGMAVERCV 139
Cdd:cd15143  30 RKTKSRSRSSFLIFLCGLVVTDFLGLLVTGTIVISFHLTNFNwrvvdPDCYLCNFMGLSMVFYGLCPLLLGATMAVERFF 109
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   140 GVTQPLIHAARVSVARARLALAVLAAMALAVALLPLVHVGRYELQYPGTWCFISLGPRGGwrQALLAGLFAGLGLAALLA 219
Cdd:cd15143 110 GINRPFSRSTAMSKRRAWYMVGMVWAFAFLLGLLPILGLGRYTLQYPGSWCFLTLLFDSK--DVAFGLLFSFLGILSVGL 187
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   220 ALVCNTLSGLALlrarwrrrrsrrfrktagpddrrrwgsrgprlasassassitsatatLRSSRGGGSARRVHAHDVEMV 299
Cdd:cd15143 188 SFLLNTVSVVTL-----------------------------------------------CRVYHDRESVQRRRDSEVEMM 220
                       250       260       270       280       290       300       310
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 548475   300 GQLVGIMVVSCICWSPLLVLVV---------LAIGGWNSNSLQRPLFLAVRLASWNQILDPWVYILLRQAM 361
Cdd:cd15143 221 VQLLGIMVIASVCWLPLLVFIAqtvlqkppaMLLAGQIPRLTEKKLLIYLRFATWNQILDPWVYILFRRAV 291
7tmA_PGE2_EP3 cd15146
prostaglandin E2 receptor EP3 subtype, member of the class A family of seven-transmembrane G ...
37-359 1.04e-29

prostaglandin E2 receptor EP3 subtype, member of the class A family of seven-transmembrane G protein-coupled receptors; Prostaglandin E2 receptor EP3, also called prostanoid EP3 receptor, is one of four receptor subtypes whose endogenous physiological ligand is prostaglandin E2 (PGE2). Each of these subtypes (EP1-EP4) have unique but overlapping tissue distributions that activate different intracellular signaling pathways. Stimulation of the EP3 receptor by PGE2 preferentially couples to G(i) protein. This leads to a decrease in adenylate cyclase activity, thereby decreasing cAMP levels, which subsequently produces smooth muscle contraction. Knockout mice studies suggest that the EP3 receptor may act as a systemic vasopressor. Prostanoids are the cyclooxygenase (COX) metabolites of arachidonic acid, which include the prostaglandins (PGD2, PGE2, PGF2alpha), prostacyclin (PGI2), and thromboxane A2 (TxA2). These five major bioactive prostanoids acts as mediators or modulators in a wide range of physiological and pathophysiological processes within the kidney and play important roles in inflammation, platelet aggregation, and vasoconstriction/relaxation, among many others. They act locally by preferentially interacting with G protein-coupled receptors designated DP, EP. FP, IP, and TP, respectively. The phylogenetic tree suggests that the prostanoid receptors can be grouped into two major branches: G(s)-coupled (DP1, EP2, EP4, and IP) and G(i)- (EP3) or G(q)-coupled (EP1, FP, and TP), forming three clusters.


Pssm-ID: 320274 [Multi-domain]  Cd Length: 308  Bit Score: 116.90  E-value: 1.04e-29
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475    37 SPALPIFSMTLGAVSNVLALALLAQVAgRMRRRRSAATFLLFVASLLAIDLAGHVIPGALVLRLYTAGRA-----PAGGA 111
Cdd:cd15146   3 SVAFPITMMITGMVGNALAMLLVYRSY-RKKENKRKRSFLLCIGSLALTDLFGQLLTSPIVISVYLSDRKwervdPSGRL 81
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   112 CHFLGGCMVFFGLCPLLLGCGMAVERCVGVTQPLIHAARVSVARARLALAVLAAMALAVALLPLVHVGRYELQYPGTWCF 191
Cdd:cd15146  82 CPFFGLCMTVFGLCPLFIASAMAIERALAIRAPHWYSSHMKTRVTKAVLLGIWLAVLAFALLPIAGVGQYTLQWPGTWCF 161
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   192 ISLGpRGGWRQALLAGLFAGLGLAALLAALVCNTLSGLALLrarwrrrrSRRFRKTAGPDDRRRWGsrgpRLAsassass 271
Cdd:cd15146 162 ISTG-DGEPGNNFFASTFASLGLFSLCVTFSCNLATIRALV--------SRCKTKAGASVSSKQWE----RIT------- 221
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   272 itsatatlrssrgggsarrvhahdVEMVGQLVGIMVVSCICWSPLLVLVVLAIGGWNSNSLQRP---------------L 336
Cdd:cd15146 222 ------------------------TETLIQLLGIMCVLSACWSPLLVLMLKMISNHTSSEHCKSatappqstelqkdcnF 277
                       330       340
                ....*....|....*....|....
gi 548475   337 FL-AVRLASWNQILDPWVYILLRQ 359
Cdd:cd15146 278 FLtAVRLASLNQILDPWVYLLLRK 301
7tmA_PGE2_EP4 cd15142
prostaglandin E2 receptor EP4 subtype, member of the class A family of seven-transmembrane G ...
35-361 1.52e-23

prostaglandin E2 receptor EP4 subtype, member of the class A family of seven-transmembrane G protein-coupled receptors; Prostaglandin E2 receptor EP4, also called prostanoid EP4 receptor, is one of four receptor subtypes whose endogenous physiological ligand is prostaglandin E2 (PGE2). Each of these subtypes (EP1-EP4) have unique but overlapping tissue distributions that activate different intracellular signaling pathways. Like the EP2 receptor, stimulation of the EP4 receptor by PGE2 causes cAMP accumulation through G(s) protein activation. Knockout studies in mice suggest that EP4 receptor may be involved in the maintenance of bone mass and fracture healing. Prostanoids are the cyclooxygenase (COX) metabolites of arachidonic acid, which include the prostaglandins (PGD2, PGE2, PGF2alpha), prostacyclin (PGI2), and thromboxane A2 (TxA2). These five major bioactive prostanoids acts as mediators or modulators in a wide range of physiological and pathophysiological processes within the kidney and play important roles in inflammation, platelet aggregation, and vasoconstriction/relaxation, among many others. They act locally by preferentially interacting with G protein-coupled receptors designated DP, EP. FP, IP, and TP, respectively. The phylogenetic tree suggests that the prostanoid receptors can be grouped into two major branches: G(s)-coupled (DP1, EP2, EP4, and IP) and G(i)- (EP3) or G(q)-coupled (EP1, FP, and TP), forming three clusters.


Pssm-ID: 320270 [Multi-domain]  Cd Length: 302  Bit Score: 99.50  E-value: 1.52e-23
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475    35 GTSPALPIFSMTLGAVSNVLALALLAqvagRMRRRRSAATFLLFVASLLAIDLAGHVIPGALVLRLYTAGRAPAGGA-CH 113
Cdd:cd15142   1 ATPPTIPAVMFIFGVVGNLIAIVVLC----KSRKEQKETTFYTLVCGLAVTDLLGTCLASPVTIATYLKGRWPGGQPlCE 76
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   114 FLGGCMVFFGLCPLLLGCGMAVERCVGVTQPLIHAARVSVARARLALAVLAAMALAVALLPLVHVGRYELQYPGTWCFIS 193
Cdd:cd15142  77 YFSFILLFFSLSGLSIICAMSIERYLAINHAYFYNHYVDKRLAGLTLFAIYASNILFCALPSMGLGKSKLQYPKTWCFID 156
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   194 lgprggWRQALLAGLF-----AGLGLAALLAALVCNTLSGLALLRARWRRRRSRRFRKTAGPDDRRRWGSRgprlasass 268
Cdd:cd15142 157 ------WRTNVSVHAAysymyAGFSSLLILVTVLCNVLVCGALIRMHRQFVRRTSLGTDQRLSDFRRRRSF--------- 221
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   269 assitsatatlrssrgggsaRRVHAHDVEMVGQLVGIMVVSCICWSPLLVLVVLaiggwnsNSLQRPLF----------L 338
Cdd:cd15142 222 --------------------RRMAGAEIQMVILLIATSVVVLICSIPLVVRVFV-------NQLYQPAVekdldknpdlQ 274
                       330       340
                ....*....|....*....|...
gi 548475   339 AVRLASWNQILDPWVYILLRQAM 361
Cdd:cd15142 275 AIRIASVNPILDPWIYILLRKTV 297
7tmA_PGE2_EP2 cd15139
prostaglandin E2 receptor EP2 subtype, member of the class A family of seven-transmembrane G ...
35-358 4.11e-14

prostaglandin E2 receptor EP2 subtype, member of the class A family of seven-transmembrane G protein-coupled receptors; Prostaglandin E2 receptor EP2, also called prostanoid EP2 receptor, is one of four receptor subtypes whose endogenous physiological ligand is prostaglandin E2 (PGE2). Each of these subtypes (EP1-EP4) have unique but overlapping tissue distributions that activate different intracellular signaling pathways. Stimulation of the EP2 receptor by PGE2 causes cAMP accumulation through G(s) protein activation, which subsequently produces smooth muscle relaxation and mediates the systemic vasodepressor response to PGE2. Prostanoids are the cyclooxygenase (COX) metabolites of arachidonic acid, which include the prostaglandins (PGD2, PGE2, PGF2alpha), prostacyclin (PGI2), and thromboxane A2 (TxA2). These five major bioactive prostanoids acts as mediators or modulators in a wide range of physiological and pathophysiological processes within the kidney and play important roles in inflammation, platelet aggregation, and vasoconstriction/relaxation, among many others. They act locally by preferentially interacting with G protein-coupled receptors designated DP, EP. FP, IP, and TP, respectively. The phylogenetic tree suggests that the prostanoid receptors can be grouped into two major branches: G(s)-coupled (DP1, EP2, EP4, and IP) and G(i)- (EP3) or G(q)-coupled (EP1, FP, and TP), forming three clusters.


Pssm-ID: 320267 [Multi-domain]  Cd Length: 299  Bit Score: 72.50  E-value: 4.11e-14
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475    35 GTSPALPIFSMTLGAVSNVLALALLAQVAgRMRRRRSAATFLLFVASLLAIDLAGHVIPGALVLRLYTA-----GRAPAG 109
Cdd:cd15139   1 GGSPAISALMFSAGVLGNVLALVLLERRR-RKEVGRRQSLFHVLVTSLVITDLLGTCLISPVVLASYSRnttlvGMSPNR 79
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   110 GACHFLGGCMVFFGLCPLLLGCGMAVERCVGVTQPLIHAARVSVARARLALAVLAAMALAVALLPLVHVGRYeLQY-PGT 188
Cdd:cd15139  80 LVCGYFGFAMTFFSLATMLILLAMALERCLSIGHPYFYERYVSKRCGYVTIPLIYLLCALFCLFPFLGFGKY-VQYcPGT 158
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   189 WCFISLGPRGGWRQALLAGLFAGLGLAALLAAlVCNTLSGLALLRARwrrrrsrrfrktagpddRRRWGSRgprlasass 268
Cdd:cd15139 159 WCFIDMNPEASEHRAYANLYATLLLLLIVAVV-LCNASVIYHLVRMY-----------------RRRKRNR--------- 211
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   269 assitsatatlRSSRGGGSARRVH---AHDVEMVGQLVGIMVVSCICWSPLLVLVVLAIGGWNSNslQRPLFLAVRLASW 345
Cdd:cd15139 212 -----------SSVGGRARSHRRRfsmAEEVEHLILLVFMTIIFVICSLPLTIQVYMNTFSDSEN--HKWDLIALRFLSV 278
                       330
                ....*....|...
gi 548475   346 NQILDPWVYILLR 358
Cdd:cd15139 279 NPIIDPWVFIILS 291
7tmA_PGI2 cd15141
prostaglandin I2 receptor, member of the class A family of seven-transmembrane G ...
65-361 6.16e-13

prostaglandin I2 receptor, member of the class A family of seven-transmembrane G protein-coupled receptors; Prostaglandin I2 receptor (also called prostacyclin receptor or prostanoid IP receptor) is a class A, G protein-coupled receptor whose endogenous ligand is prostacyclin, which is the major product of cyclooxygenase metabolite of arachidonic acid that found predominantly in platelets and vascular smooth muscle cells (VSMCs). The PGI2 receptor is coupled to both G(s) and G(q) protein subtypes, resulting in increased cAMP formation, phosphoinositide turnover, and Ca2+ signaling. PGI2 receptor activation by prostacyclin induces VSMC differentiation and produces a potent vasodilation and inhibition of platelet aggregation.


Pssm-ID: 320269 [Multi-domain]  Cd Length: 301  Bit Score: 69.08  E-value: 6.16e-13
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475    65 RMRRRRSAATFLLFVASLLAIDLAGHVIPGALVLRLYT-----AGRAPAGGACHFLGGCMVFFGLCPLLLGCGMAVERCV 139
Cdd:cd15141  29 RKERRTKSSAFCVLVTGLAATDLLGTCFLSPMVFVSYAqnsslLGLAAGQPLCHLFAFAMTFFGLASMLILFAMAVERCL 108
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   140 GVTQPLIHAARVSVARARLALAVLAAMALAVALLPLVHVGRYELQYPGTWCFISLGPRGGWRQALLAGLFAGLGLAALLA 219
Cdd:cd15141 109 AISHPYFYAQHSGRRLAKLALPAIYAFGALFCALPLLGVGRHKQYCPGTWCFIRMTVPGHREPGSLAFSLLYASLMALLI 188
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475   220 ALV--CNTLSGLALLRARWRrrrsrrfrktagpdDRRRWGSrgprlasassassitsatatLRSSRGGGSARRVHAHDVE 297
Cdd:cd15141 189 AAIflCNGSVTVSLCRMYRG--------------QKARRGS--------------------LRRCGRLGWWLGQGEEEVD 234
                       250       260       270       280       290       300
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 548475   298 MVGQLVGIMVVSCICWSPLLVLVVlaIGGWNSNSLQRPLFLAVRLASWNQILDPWVYILLRQAM 361
Cdd:cd15141 235 HLILLALMTVIFVVCSLPLTIRAF--LGAIAPDGNESGDLLAFRFSAFNPILDPWIFIIFRKAV 296
7tmA_PGD2 cd15140
prostaglandin D2 receptor, member of the class A family of seven-transmembrane G ...
69-194 4.82e-05

prostaglandin D2 receptor, member of the class A family of seven-transmembrane G protein-coupled receptors; Prostaglandin D2 receptor (also called prostanoid DP receptor, DP1, or PGD2R1) is a G-protein coupled receptor whose endogenous ligand is prostaglandin D2 (PGD2). PGD2, the major cyclooxygenase metabolite of arachidonic acid produced by mast cells, mediates inflammatory reactions in response to allergen challenge and causes peripheral vasodilation. PGD2 exerts its biological effects by binding to two types of cell surface receptors: a DP1 receptor that belongs to the prostanoid receptor family and a chemoattractant receptor-homologous molecule expressed on the T-helper type 2 cells (CRTH2 or PD2R2).


Pssm-ID: 320268 [Multi-domain]  Cd Length: 312  Bit Score: 44.89  E-value: 4.82e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 548475    69 RRSAATFLLFVASLLAIDLAGHVIPGALVLRLYTAGR-----APAGGA--CHFLGGCMVFFGLCPLLLGCGMAVERCVGV 141
Cdd:cd15140  39 RPRTSVFYVLVTVLTVTDLLGKCLLSPVVLAAYAQNRslvglTPAGNLnlCQVFAFLMIFFGLASTFILLAMALECWLSL 118
                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|...
gi 548475   142 TQPLIHAARVSVARARLALAVLAAMALAVALLPLVHVGRYELQYPGTWCFISL 194
Cdd:cd15140 119 GHPFFYQRHINKRLGALVSLILYAFCLGFCALPFFGFGRYVQYCPGTWCFIQM 171
7tm_1 pfam00001
7 transmembrane receptor (rhodopsin family); This family contains, amongst other ...
280-354 6.47e-04

7 transmembrane receptor (rhodopsin family); This family contains, amongst other G-protein-coupled receptors (GCPRs), members of the opsin family, which have been considered to be typical members of the rhodopsin superfamily. They share several motifs, mainly the seven transmembrane helices, GCPRs of the rhodopsin superfamily. All opsins bind a chromophore, such as 11-cis-retinal. The function of most opsins other than the photoisomerases is split into two steps: light absorption and G-protein activation. Photoisomerases, on the other hand, are not coupled to G-proteins - they are thought to generate and supply the chromophore that is used by visual opsins.


Pssm-ID: 459624 [Multi-domain]  Cd Length: 256  Bit Score: 41.13  E-value: 6.47e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 548475     280 RSSRGGGSARRVhahdVEMVGQLVGIMVVsciCWSPLLVLVVLAIGGWN---SNSLQRPLFLAVRLASWNQILDPWVY 354
Cdd:pfam00001 186 KSSERTQRRRKA----LKTLAVVVVVFIL---CWLPYHIVNLLDSLALDcelSRLLDKALSVTLWLAYVNSCLNPIIY 256
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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