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Conserved domains on  [gi|81170923|sp|P0ABN1|]
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RecName: Full=Diacylglycerol kinase; Short=DAGK; AltName: Full=Diglyceride kinase; Short=DGK

Protein Classification

diacylglycerol kinase( domain architecture ID 10198050)

diacylglycerol kinase catalyzes the ATP-dependent phosphorylation of sn-l,2-diacylglycerol (DAG) to phosphatidic acid

EC:  2.7.1.107
Gene Ontology:  GO:0008654|GO:0046872|GO:0005524|GO:0004143|GO:0006654|GO:0016310
PubMed:  22224599
SCOP:  3001940

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DAGK_IM cd14264
Integral membrane diacylglycerol kinase; This mostly bacterial family of homo-trimeric ...
 9-117 2.61e-50

Integral membrane diacylglycerol kinase; This mostly bacterial family of homo-trimeric integral membrane enzymes, the products of the dgkA gene, catalyzes the ATP-dependent phosphorylation of diacylglycerol to phosphatidic acid. Escherichia coli DAGK participates in the membrane-derived oligosaccharide cycle (MDO cycle) by recycling lipids to restore phosphatidylglycerols that were used up in the biosynthesis of MDOs. DAGK also recycles diacylglycerols that are produced during the biosynthesis of lipopolysaccharides (LPS) back to phospholipids. DAGK is not the main source of phosphatidic acid in de-novo biosynthesis of glycerophospholipids. Escherichia coli DAGK has low activity as an undecaprenol kinase.


:

Pssm-ID: 260133  Cd Length: 109  Bit Score: 154.90  E-value: 2.61e-50
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81170923   9 TRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYH 88
Cdd:cd14264   1 KRLIKATGYSLQGLKAAWKNEAAFRQELLLALILIPLAFFLGVSLLERLLLIGSLLLVLIVELLNSAIEAVVDRISLEHH 80

                        90       100
                ....*....|....*....|....*....
gi 81170923  89 ELSGRAKDMGSAAVLIAIIVAVITWCILL 117
Cdd:cd14264  81 ELSKRAKDLGSAAVFLSLLLAALVWLAIL 109
 
Name Accession Description Interval E-value
DAGK_IM cd14264
Integral membrane diacylglycerol kinase; This mostly bacterial family of homo-trimeric ...
 9-117 2.61e-50

Integral membrane diacylglycerol kinase; This mostly bacterial family of homo-trimeric integral membrane enzymes, the products of the dgkA gene, catalyzes the ATP-dependent phosphorylation of diacylglycerol to phosphatidic acid. Escherichia coli DAGK participates in the membrane-derived oligosaccharide cycle (MDO cycle) by recycling lipids to restore phosphatidylglycerols that were used up in the biosynthesis of MDOs. DAGK also recycles diacylglycerols that are produced during the biosynthesis of lipopolysaccharides (LPS) back to phospholipids. DAGK is not the main source of phosphatidic acid in de-novo biosynthesis of glycerophospholipids. Escherichia coli DAGK has low activity as an undecaprenol kinase.


Pssm-ID: 260133  Cd Length: 109  Bit Score: 154.90  E-value: 2.61e-50
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81170923   9 TRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYH 88
Cdd:cd14264   1 KRLIKATGYSLQGLKAAWKNEAAFRQELLLALILIPLAFFLGVSLLERLLLIGSLLLVLIVELLNSAIEAVVDRISLEHH 80

                        90       100
                ....*....|....*....|....*....
gi 81170923  89 ELSGRAKDMGSAAVLIAIIVAVITWCILL 117
Cdd:cd14264  81 ELSKRAKDLGSAAVFLSLLLAALVWLAIL 109
DgkA COG0818
Diacylglycerol kinase [Lipid transport and metabolism];
3-122 5.77e-45

Diacylglycerol kinase [Lipid transport and metabolism];


Pssm-ID: 440580  Cd Length: 120  Bit Score: 141.77  E-value: 5.77e-45
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81170923   3 NNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDR 82
Cdd:COG0818   1 KSKKKLRRLIKSFGYALRGLKAAFRTERNFRIHLVLAVLVIPLAFFLGLSAVEWALLILAIGLVLIAELLNTAIEAVVDL 80

                        90       100       110       120
                ....*....|....*....|....*....|....*....|
gi 81170923  83 IGSEYHELSGRAKDMGSAAVLIAIIVAVITWCILLWSHFG 122
Cdd:COG0818  81 VSPEYHPLAKRAKDIAAAAVLLAALLAVVVGLIILLPKLL 120
DAGK_prokar pfam01219
Prokaryotic diacylglycerol kinase;
16-117 1.33e-37

Prokaryotic diacylglycerol kinase;


Pssm-ID: 460117  Cd Length: 102  Bit Score: 122.86  E-value: 1.33e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81170923    16 GYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAK 95
Cdd:pfam01219   1 GYALRGLKAAFRTERNFRIHLVLAVLVLILAFFLGLSPVEWALLILAIGLVLAAELLNTAIEAVVDLVSPEYHPLAKRAK 80

                          90       100
                  ....*....|....*....|..
gi 81170923    96 DMGSAAVLIAIIVAVITWCILL 117
Cdd:pfam01219  81 DIAAGAVLVAAILAVIVGLLIL 102
 
Name Accession Description Interval E-value
DAGK_IM cd14264
Integral membrane diacylglycerol kinase; This mostly bacterial family of homo-trimeric ...
 9-117 2.61e-50

Integral membrane diacylglycerol kinase; This mostly bacterial family of homo-trimeric integral membrane enzymes, the products of the dgkA gene, catalyzes the ATP-dependent phosphorylation of diacylglycerol to phosphatidic acid. Escherichia coli DAGK participates in the membrane-derived oligosaccharide cycle (MDO cycle) by recycling lipids to restore phosphatidylglycerols that were used up in the biosynthesis of MDOs. DAGK also recycles diacylglycerols that are produced during the biosynthesis of lipopolysaccharides (LPS) back to phospholipids. DAGK is not the main source of phosphatidic acid in de-novo biosynthesis of glycerophospholipids. Escherichia coli DAGK has low activity as an undecaprenol kinase.


Pssm-ID: 260133  Cd Length: 109  Bit Score: 154.90  E-value: 2.61e-50
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81170923   9 TRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYH 88
Cdd:cd14264   1 KRLIKATGYSLQGLKAAWKNEAAFRQELLLALILIPLAFFLGVSLLERLLLIGSLLLVLIVELLNSAIEAVVDRISLEHH 80

                        90       100
                ....*....|....*....|....*....
gi 81170923  89 ELSGRAKDMGSAAVLIAIIVAVITWCILL 117
Cdd:cd14264  81 ELSKRAKDLGSAAVFLSLLLAALVWLAIL 109
DgkA COG0818
Diacylglycerol kinase [Lipid transport and metabolism];
3-122 5.77e-45

Diacylglycerol kinase [Lipid transport and metabolism];


Pssm-ID: 440580  Cd Length: 120  Bit Score: 141.77  E-value: 5.77e-45
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81170923   3 NNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDR 82
Cdd:COG0818   1 KSKKKLRRLIKSFGYALRGLKAAFRTERNFRIHLVLAVLVIPLAFFLGLSAVEWALLILAIGLVLIAELLNTAIEAVVDL 80

                        90       100       110       120
                ....*....|....*....|....*....|....*....|
gi 81170923  83 IGSEYHELSGRAKDMGSAAVLIAIIVAVITWCILLWSHFG 122
Cdd:COG0818  81 VSPEYHPLAKRAKDIAAAAVLLAALLAVVVGLIILLPKLL 120
DAGK_prokar pfam01219
Prokaryotic diacylglycerol kinase;
16-117 1.33e-37

Prokaryotic diacylglycerol kinase;


Pssm-ID: 460117  Cd Length: 102  Bit Score: 122.86  E-value: 1.33e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81170923    16 GYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAK 95
Cdd:pfam01219   1 GYALRGLKAAFRTERNFRIHLVLAVLVLILAFFLGLSPVEWALLILAIGLVLAAELLNTAIEAVVDLVSPEYHPLAKRAK 80

                          90       100
                  ....*....|....*....|..
gi 81170923    96 DMGSAAVLIAIIVAVITWCILL 117
Cdd:pfam01219  81 DIAAGAVLVAAILAVIVGLLIL 102
DAGK_IM_like cd14263
Integral membrane diacylglycerol kinase and similar enzymes; This mostly bacterial family of ...
 12-117 1.22e-27

Integral membrane diacylglycerol kinase and similar enzymes; This mostly bacterial family of homo-trimeric integral membrane enzymes, the products of the dgkA gene, catalyzes the ATP-dependent phosphorylation of substrates such as diacylglycerol to phosphatidic acid or of undecaprenol to undecaprenyl phosphate. They are not related other cytosolic or membrane-associated kinases, including the eukaryotic diacylglycerol kinases.


Pssm-ID: 260132  Cd Length: 106  Bit Score: 97.52  E-value: 1.22e-27
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81170923  12 IKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELS 91
Cdd:cd14263   1 RKSFRYALRGLKYAFRTERNFRIHLVAALLVLILALFLGLSLVEWALLILAIGLVLVAELINTAIERLVDLLSPEYHPLA 80

                        90       100
                ....*....|....*....|....*.
gi 81170923  92 GRAKDMGSAAVLIAIIVAVITWCILL 117
Cdd:cd14263  81 KIAKDIAAGAVLVAALAAVVVGLLIF 106
UDPK_IM_like cd14265
Integral membrane undecaprenol kinase and similar enzymes; This mostly bacterial family of ...
 12-117 9.39e-23

Integral membrane undecaprenol kinase and similar enzymes; This mostly bacterial family of homo-trimeric integral membrane enzymes, the products of the dgkA gene, catalyzes the ATP-dependent phosphorylation of undecaprenol to undecaprenyl phosphate. C55-isoprenyl (undecaprenyl) pyroposphate acts as a scaffold for the assembly of peptidoglycan components; undecaprenol kinase (UDPK) is involved in recycling undecaprenyl units for re-use in the peptidoglycan biosynthesis. UDPK does not participate in the de-novo biosynthesis of undecaprenyl phosphate. Gram-positive bacteria have a large pool of free undecaprenol, in contrast to gram-negative bacteria. UDPK may also play a role in a stress-induced pathway that affects the function of ribosomes. In Streptococcus mutans, UDPK has been shown to be required for biofilm formation, such as in the case of smooth surface dental caries. Members of the UDPK family have low activity as diacylglycerol kinases (DAGK), and many of them are annotated as DAGKs.


Pssm-ID: 260134  Cd Length: 106  Bit Score: 85.13  E-value: 9.39e-23
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81170923  12 IKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELS 91
Cdd:cd14265   1 LKSFRYAFRGIKYAFRTERNFRIHLVAAVLVIILGFFLGLSRLEWLLLLLAIGLVLAAELINTAIEALVDLVSPEYHPLA 80

                        90       100
                ....*....|....*....|....*.
gi 81170923  92 GRAKDMGSAAVLIAIIVAVITWCILL 117
Cdd:cd14265  81 KIAKDVAAGAVLVAAIFAVIVGLIIF 106
UDPK_IM_PAP2_like cd14266
Integral membrane undecaprenol kinase domain co-occurring with type 2 phosphatidic acid ...
 12-111 1.39e-10

Integral membrane undecaprenol kinase domain co-occurring with type 2 phosphatidic acid phosphatase-like domains; This bacterial family of homo-trimeric integral membrane enzyme domains catalyzes the ATP-dependent phosphorylation of of undecaprenol to undecaprenyl phosphate. They sit N-terminally to phosphatase domains that are members of the type 2 phosphatidic acid phosphatase superfamily, and the function of members of this domain architecture was determined to be undecaprenyl pyrophosphate phosphatases. The bi-functional enzymes might generate undecaprenyl phosphate via two mechanisms - the phosphorylation of undecaprenol or the cleavage of the terminal phosphate group of undecaprenyl pyrophosphate.


Pssm-ID: 260135  Cd Length: 106  Bit Score: 54.10  E-value: 1.39e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 81170923  12 IKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELS 91
Cdd:cd14266   1 IDSFNYAIEGIIYAIKTERNMKIHFLAAILVLILSLFFDLSKVELLILFITITLVIAAELINTAIEATVDLITDEYHPLA 80

                        90       100
                ....*....|....*....|
gi 81170923  92 GRAKDMGSAAVLIAIIVAVI 111
Cdd:cd14266  81 KIAKDVAAGAVLVAALNALI 100
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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