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Conserved domains on  [gi|1187477267|gb|OSP19942|]
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restriction endonuclease [Citrobacter werkmanii]

Protein Classification

HNH endonuclease( domain architecture ID 11465816)

HNH endonuclease similar to Haemophilus parahaemolyticus type II restriction enzyme HphI, which recognizes the double-stranded sequences, GGTGA and TCACC, and performs endonucleolytic cleavage of DNA to give specific double-stranded fragments

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
COG3440 COG3440
Predicted restriction endonuclease [Defense mechanisms];
134-288 1.37e-52

Predicted restriction endonuclease [Defense mechanisms];


:

Pssm-ID: 442665  Cd Length: 150  Bit Score: 169.02  E-value: 1.37e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1187477267 134 AQQLLEAHFPASIQEDIaDEMGFDIRTSLRQRDPKFRQMVLRAYNYQCAICGFNMrhdnaPIALEAAHIR-WKQNHGPCE 212
Cdd:COG3440     1 AEQLLDALFLEEEPDEL-ERTERERLVKRRVRQGAFRKAVLEAYDGRCAVTGLDI-----PPLLEAAHIKpWSDHGGRDD 74

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1187477267 213 VPNGLALCAIHHKAFDRGSIGLDENMRVMVSGAVNGGGVVQRLFWDFAGKAIALPPVKENYPGERFVEWHRKEVFR 288
Cdd:COG3440    75 VSNGLLLCPNHHALFDRGLITIDDDYRILVSPRLKEESDLIRELKELHGRKLRLPEHERFRPDPEYLAWHRENVFK 150
 
Name Accession Description Interval E-value
COG3440 COG3440
Predicted restriction endonuclease [Defense mechanisms];
134-288 1.37e-52

Predicted restriction endonuclease [Defense mechanisms];


Pssm-ID: 442665  Cd Length: 150  Bit Score: 169.02  E-value: 1.37e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1187477267 134 AQQLLEAHFPASIQEDIaDEMGFDIRTSLRQRDPKFRQMVLRAYNYQCAICGFNMrhdnaPIALEAAHIR-WKQNHGPCE 212
Cdd:COG3440     1 AEQLLDALFLEEEPDEL-ERTERERLVKRRVRQGAFRKAVLEAYDGRCAVTGLDI-----PPLLEAAHIKpWSDHGGRDD 74

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1187477267 213 VPNGLALCAIHHKAFDRGSIGLDENMRVMVSGAVNGGGVVQRLFWDFAGKAIALPPVKENYPGERFVEWHRKEVFR 288
Cdd:COG3440    75 VSNGLLLCPNHHALFDRGLITIDDDYRILVSPRLKEESDLIRELKELHGRKLRLPEHERFRPDPEYLAWHRENVFK 150
HNH_2 pfam13391
HNH endonuclease;
181-235 1.35e-09

HNH endonuclease;


Pssm-ID: 433168  Cd Length: 66  Bit Score: 53.56  E-value: 1.35e-09
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1187477267 181 CAICGFNmrhdnAPIALEAAHIR-WKQNHGPC---------------EVPNGLALCAIHHKAFDRGSIGLD 235
Cdd:pfam13391   1 CVITGID-----APGLLEAAHIFpWSLSLGDRrfsairlgegpsgidSPRNGLLLRPDLHRLFDRGLFAID 66
 
Name Accession Description Interval E-value
COG3440 COG3440
Predicted restriction endonuclease [Defense mechanisms];
134-288 1.37e-52

Predicted restriction endonuclease [Defense mechanisms];


Pssm-ID: 442665  Cd Length: 150  Bit Score: 169.02  E-value: 1.37e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1187477267 134 AQQLLEAHFPASIQEDIaDEMGFDIRTSLRQRDPKFRQMVLRAYNYQCAICGFNMrhdnaPIALEAAHIR-WKQNHGPCE 212
Cdd:COG3440     1 AEQLLDALFLEEEPDEL-ERTERERLVKRRVRQGAFRKAVLEAYDGRCAVTGLDI-----PPLLEAAHIKpWSDHGGRDD 74

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1187477267 213 VPNGLALCAIHHKAFDRGSIGLDENMRVMVSGAVNGGGVVQRLFWDFAGKAIALPPVKENYPGERFVEWHRKEVFR 288
Cdd:COG3440    75 VSNGLLLCPNHHALFDRGLITIDDDYRILVSPRLKEESDLIRELKELHGRKLRLPEHERFRPDPEYLAWHRENVFK 150
HNH_2 pfam13391
HNH endonuclease;
181-235 1.35e-09

HNH endonuclease;


Pssm-ID: 433168  Cd Length: 66  Bit Score: 53.56  E-value: 1.35e-09
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1187477267 181 CAICGFNmrhdnAPIALEAAHIR-WKQNHGPC---------------EVPNGLALCAIHHKAFDRGSIGLD 235
Cdd:pfam13391   1 CVITGID-----APGLLEAAHIFpWSLSLGDRrfsairlgegpsgidSPRNGLLLRPDLHRLFDRGLFAID 66
COG3183 COG3183
Predicted restriction endonuclease, HNH family [Defense mechanisms];
163-243 3.41e-06

Predicted restriction endonuclease, HNH family [Defense mechanisms];


Pssm-ID: 442416 [Multi-domain]  Cd Length: 112  Bit Score: 44.99  E-value: 3.41e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1187477267 163 RQRDPKFRQMVLRAYNYQCAICGFNMRHDNAPIA---LEAAHIRW--KQNHGPCEVPNGL-ALCAIHHKAFDRGSIGLD- 235
Cdd:COG3183    21 YERNPKLRKKAKEHAGGKCEVCGFDFEFVYGELGegyIEVHHLIPlsEIGEGGVDPIEDLvPLCPNCHRMLHRGRPPLSi 100


                  ....*...
gi 1187477267 236 ENMRVMVS 243
Cdd:COG3183   101 EELKKLLK 108
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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