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Conserved domains on  [gi|1032289633|gb|OAP03960|]
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hypothetical protein AXX17_AT3G50080 [Arabidopsis thaliana]

Protein Classification

DNA replication complex GINS protein PSF3( domain architecture ID 16902678)

DNA replication complex GINS protein PSF3 is a component of the heterotetrameric GINS complex; the GINS complex is essential for both the initiation and elongation stages of eukaryotic DNA replication

CATH:  1.20.58.2050
Gene Ontology:  GO:0006260
PubMed:  22918584|20070258

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GINS_A_psf3 cd11713
Alpha-helical domain of GINS complex protein Psf3 (partner of Sld5 3); Psf3 is a component of ...
 61-167 4.08e-31

Alpha-helical domain of GINS complex protein Psf3 (partner of Sld5 3); Psf3 is a component of GINS, a tetrameric protein complex. Psf3 expression is up regulated in malignant colon cancer and it might be involved in cancer cell proliferation. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits are homologous and homologs are also found in the archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


:

Pssm-ID: 212551  Cd Length: 109  Bit Score: 108.89  E-value: 4.08e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032289633  61 VTINLPPCFDQKTRLEVQADAAYVDLRSRCPYFYEFGCKIEPLVT-DRTLGILLSTAFKIRYKEALTKVYTAAHITASKY 139
Cdd:cd11713     2 VSVELPKIYSEKVRNALKADPTSVDLHKLSPYFYELGLKLLSLFPeDEELAEVLLETFKERFREIMDHAQNASNEDNSEF 81

                          90       100
                  ....*....|....*....|....*...
gi 1032289633 140 LSFLTKEETNLYEAAHLSMTAFKKWRTG 167
Cdd:cd11713    82 LRKLDELERKLFRAGQESLKDFKKWLKG 109
GINS_B_Psf3 cd21693
beta-strand (B) domain of GINS complex protein Psf3; Psf3 (partner of Sld5 3) is one of the ...
 4-66 6.61e-25

beta-strand (B) domain of GINS complex protein Psf3; Psf3 (partner of Sld5 3) is one of the proteins known to comprise the GINS (named from the Japanese go-ichi-ni-san, meaning 5-1-2-3 for the Sld5, Psf1, Psf2, and Psf3 subunits) complex, which is a macromolecular protein complex associated with DNA replication. Psf3 is dysregulated in cancer cells, and its overexpression may be related to tumor progression in some cancers including colon, breast, and lung cancers; its expression can be used as a prognostic indicator in some cancers. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both the initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits Sld5, Psf1, Psf2, and Psf3 are homologous, and homologs are also found in archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, called the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3. This model represents the B-domain of GINS subunit Psf3.


:

Pssm-ID: 412029  Cd Length: 64  Bit Score: 91.45  E-value: 6.61e-25
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1032289633   4 YFDIDDILIEEEFVPVLFHKTANGV-TIDPSAETNCAEQGSKVELPFWLAHELHLRQAVTINLP 66
Cdd:cd21693     1 YYDIDDILAEEEKVPCRFEVDLPGLgFLDPSSDDDDLPPGTKVELPLWLAKALARRRIVSVELP 64
 
Name Accession Description Interval E-value
GINS_A_psf3 cd11713
Alpha-helical domain of GINS complex protein Psf3 (partner of Sld5 3); Psf3 is a component of ...
 61-167 4.08e-31

Alpha-helical domain of GINS complex protein Psf3 (partner of Sld5 3); Psf3 is a component of GINS, a tetrameric protein complex. Psf3 expression is up regulated in malignant colon cancer and it might be involved in cancer cell proliferation. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits are homologous and homologs are also found in the archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


Pssm-ID: 212551  Cd Length: 109  Bit Score: 108.89  E-value: 4.08e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032289633  61 VTINLPPCFDQKTRLEVQADAAYVDLRSRCPYFYEFGCKIEPLVT-DRTLGILLSTAFKIRYKEALTKVYTAAHITASKY 139
Cdd:cd11713     2 VSVELPKIYSEKVRNALKADPTSVDLHKLSPYFYELGLKLLSLFPeDEELAEVLLETFKERFREIMDHAQNASNEDNSEF 81

                          90       100
                  ....*....|....*....|....*...
gi 1032289633 140 LSFLTKEETNLYEAAHLSMTAFKKWRTG 167
Cdd:cd11713    82 LRKLDELERKLFRAGQESLKDFKKWLKG 109
GINS_B_Psf3 cd21693
beta-strand (B) domain of GINS complex protein Psf3; Psf3 (partner of Sld5 3) is one of the ...
 4-66 6.61e-25

beta-strand (B) domain of GINS complex protein Psf3; Psf3 (partner of Sld5 3) is one of the proteins known to comprise the GINS (named from the Japanese go-ichi-ni-san, meaning 5-1-2-3 for the Sld5, Psf1, Psf2, and Psf3 subunits) complex, which is a macromolecular protein complex associated with DNA replication. Psf3 is dysregulated in cancer cells, and its overexpression may be related to tumor progression in some cancers including colon, breast, and lung cancers; its expression can be used as a prognostic indicator in some cancers. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both the initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits Sld5, Psf1, Psf2, and Psf3 are homologous, and homologs are also found in archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, called the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3. This model represents the B-domain of GINS subunit Psf3.


Pssm-ID: 412029  Cd Length: 64  Bit Score: 91.45  E-value: 6.61e-25
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1032289633   4 YFDIDDILIEEEFVPVLFHKTANGV-TIDPSAETNCAEQGSKVELPFWLAHELHLRQAVTINLP 66
Cdd:cd21693     1 YYDIDDILAEEEKVPCRFEVDLPGLgFLDPSSDDDDLPPGTKVELPLWLAKALARRRIVSVELP 64
COG5138 COG5138
Uncharacterized conserved protein [Function unknown];
4-164 1.34e-18

Uncharacterized conserved protein [Function unknown];


Pssm-ID: 227467 [Multi-domain]  Cd Length: 168  Bit Score: 78.41  E-value: 1.34e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032289633   4 YFDIDDILIEEEFVPVLF-HKTANGVTIDPsaETNCAEQGSKVELPFWLAHELHLRQAVTINLPPCFDQKTRLEVQADAA 82
Cdd:COG5138     3 YYDIDDILLGESRIPCTFkHKIPGLGFLGP--RGRILPENRKVELPYWLASFLAINSFVCELGPGFFSESVLNAIKAKPS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032289633  83 YVDLRSRCPYFYEFGCKIEPLVTDRTLGILLSTAFKIRYKEaltkvYTAAHITASKYLS----FLTK---EETNLYEAAH 155
Cdd:COG5138    81 TVDLRSICPHFFYLAEKYAMLLSEGSLVAGLPEFLYERAGD-----YSSASLNLKGTFSensqFVWRldmVEKSIVVGSH 155


                  ....*....
gi 1032289633 156 LSMTAFKKW 164
Cdd:COG5138   156 RRFQRFCAW 164
Sld5 pfam05916
GINS complex protein; The eukaryotic GINS complex is essential for the initiation and ...
 45-147 7.74e-15

GINS complex protein; The eukaryotic GINS complex is essential for the initiation and elongation phases of DNA replication. It consists of four paralogous protein subunits (Sld5, Psf1, Psf2 and Psf3), all of which are included in this family. The GINS complex is conserved from yeast to humans, and has been shown in human to bind directly to DNA primase.


Pssm-ID: 399129  Cd Length: 105  Bit Score: 66.75  E-value: 7.74e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032289633  45 VELPFWLAHELHLRQAVTINLPPCFDQKTRLEVQADAAYVDLRSRCPYFYEFGCKIEPLVTDRTLGILLSTAFKIRYKEA 124
Cdd:pfam05916   1 PELPLWLAELLKRRGLVEIEPPEWLSIEELEAILADETKNDLSLLPPYFYELAVLLLELERDDELKRLLRDYLRIRLAKI 80

                          90       100
                  ....*....|....*....|....
gi 1032289633 125 LTKVYTAA-HITASKYLSFLTKEE 147
Cdd:pfam05916  81 RKLAWHLNgSLSPSDLLDNLSEEE 104
 
Name Accession Description Interval E-value
GINS_A_psf3 cd11713
Alpha-helical domain of GINS complex protein Psf3 (partner of Sld5 3); Psf3 is a component of ...
 61-167 4.08e-31

Alpha-helical domain of GINS complex protein Psf3 (partner of Sld5 3); Psf3 is a component of GINS, a tetrameric protein complex. Psf3 expression is up regulated in malignant colon cancer and it might be involved in cancer cell proliferation. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits are homologous and homologs are also found in the archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


Pssm-ID: 212551  Cd Length: 109  Bit Score: 108.89  E-value: 4.08e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032289633  61 VTINLPPCFDQKTRLEVQADAAYVDLRSRCPYFYEFGCKIEPLVT-DRTLGILLSTAFKIRYKEALTKVYTAAHITASKY 139
Cdd:cd11713     2 VSVELPKIYSEKVRNALKADPTSVDLHKLSPYFYELGLKLLSLFPeDEELAEVLLETFKERFREIMDHAQNASNEDNSEF 81

                          90       100
                  ....*....|....*....|....*...
gi 1032289633 140 LSFLTKEETNLYEAAHLSMTAFKKWRTG 167
Cdd:cd11713    82 LRKLDELERKLFRAGQESLKDFKKWLKG 109
GINS_B_Psf3 cd21693
beta-strand (B) domain of GINS complex protein Psf3; Psf3 (partner of Sld5 3) is one of the ...
 4-66 6.61e-25

beta-strand (B) domain of GINS complex protein Psf3; Psf3 (partner of Sld5 3) is one of the proteins known to comprise the GINS (named from the Japanese go-ichi-ni-san, meaning 5-1-2-3 for the Sld5, Psf1, Psf2, and Psf3 subunits) complex, which is a macromolecular protein complex associated with DNA replication. Psf3 is dysregulated in cancer cells, and its overexpression may be related to tumor progression in some cancers including colon, breast, and lung cancers; its expression can be used as a prognostic indicator in some cancers. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both the initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits Sld5, Psf1, Psf2, and Psf3 are homologous, and homologs are also found in archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, called the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3. This model represents the B-domain of GINS subunit Psf3.


Pssm-ID: 412029  Cd Length: 64  Bit Score: 91.45  E-value: 6.61e-25
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1032289633   4 YFDIDDILIEEEFVPVLFHKTANGV-TIDPSAETNCAEQGSKVELPFWLAHELHLRQAVTINLP 66
Cdd:cd21693     1 YYDIDDILAEEEKVPCRFEVDLPGLgFLDPSSDDDDLPPGTKVELPLWLAKALARRRIVSVELP 64
COG5138 COG5138
Uncharacterized conserved protein [Function unknown];
4-164 1.34e-18

Uncharacterized conserved protein [Function unknown];


Pssm-ID: 227467 [Multi-domain]  Cd Length: 168  Bit Score: 78.41  E-value: 1.34e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032289633   4 YFDIDDILIEEEFVPVLF-HKTANGVTIDPsaETNCAEQGSKVELPFWLAHELHLRQAVTINLPPCFDQKTRLEVQADAA 82
Cdd:COG5138     3 YYDIDDILLGESRIPCTFkHKIPGLGFLGP--RGRILPENRKVELPYWLASFLAINSFVCELGPGFFSESVLNAIKAKPS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032289633  83 YVDLRSRCPYFYEFGCKIEPLVTDRTLGILLSTAFKIRYKEaltkvYTAAHITASKYLS----FLTK---EETNLYEAAH 155
Cdd:COG5138    81 TVDLRSICPHFFYLAEKYAMLLSEGSLVAGLPEFLYERAGD-----YSSASLNLKGTFSensqFVWRldmVEKSIVVGSH 155


                  ....*....
gi 1032289633 156 LSMTAFKKW 164
Cdd:COG5138   156 RRFQRFCAW 164
Sld5 pfam05916
GINS complex protein; The eukaryotic GINS complex is essential for the initiation and ...
 45-147 7.74e-15

GINS complex protein; The eukaryotic GINS complex is essential for the initiation and elongation phases of DNA replication. It consists of four paralogous protein subunits (Sld5, Psf1, Psf2 and Psf3), all of which are included in this family. The GINS complex is conserved from yeast to humans, and has been shown in human to bind directly to DNA primase.


Pssm-ID: 399129  Cd Length: 105  Bit Score: 66.75  E-value: 7.74e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032289633  45 VELPFWLAHELHLRQAVTINLPPCFDQKTRLEVQADAAYVDLRSRCPYFYEFGCKIEPLVTDRTLGILLSTAFKIRYKEA 124
Cdd:pfam05916   1 PELPLWLAELLKRRGLVEIEPPEWLSIEELEAILADETKNDLSLLPPYFYELAVLLLELERDDELKRLLRDYLRIRLAKI 80

                          90       100
                  ....*....|....*....|....
gi 1032289633 125 LTKVYTAA-HITASKYLSFLTKEE 147
Cdd:pfam05916  81 RKLAWHLNgSLSPSDLLDNLSEEE 104
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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