Aldolase superfamily protein [Arabidopsis thaliana]
beta/alpha barrel domain-containing protein( domain architecture ID 229392)
beta/alpha barrel domain-containing protein belongs to a large superfamily with a wide variety of enzymatic functions
List of domain hits
Name | Accession | Description | Interval | E-value | ||||
TIM super family | cl21457 | TIM-like beta/alpha barrel domains; A large family of domains similar to triose phosphate ... |
122-350 | 5.47e-151 | ||||
TIM-like beta/alpha barrel domains; A large family of domains similar to triose phosphate isomerase (TIM) which, in general, share an eight beta/alpha closed barrel structure. The actual alignment was detected with superfamily member PLN02746: Pssm-ID: 473867 Cd Length: 347 Bit Score: 428.82 E-value: 5.47e-151
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Name | Accession | Description | Interval | E-value | ||||
PLN02746 | PLN02746 | hydroxymethylglutaryl-CoA lyase |
122-350 | 5.47e-151 | ||||
hydroxymethylglutaryl-CoA lyase Pssm-ID: 178347 Cd Length: 347 Bit Score: 428.82 E-value: 5.47e-151
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DRE_TIM_HMGL | cd07938 | 3-hydroxy-3-methylglutaryl-CoA lyase, catalytic TIM barrel domain; ... |
170-347 | 2.03e-109 | ||||
3-hydroxy-3-methylglutaryl-CoA lyase, catalytic TIM barrel domain; 3-hydroxy-3-methylglutaryl-CoA lyase (HMGL) catalyzes the cleavage of HMG-CoA to acetyl-CoA and acetoacetate, one of the terminal steps in ketone body generation and leucine degradation, and is a key enzyme in the pathway that supplies metabolic fuel to extrahepatic tissues. Mutations in HMGL cause a human autosomal recessive disorder called primary metabolic aciduria that affects ketogenesis and leucine catabolism and can be fatal due to an inability to tolerate hypoglycemia. HMGL has a TIM barrel domain with a catalytic center containing a divalent cation-binding site formed by a cluster of invariant residues that cap the core of the barrel. The cleavage of HMG-CoA requires the presence of a divalent cation like Mg2+ or Mn2+, and the reaction is thought to involve general acid/base catalysis. This family belongs to the DRE-TIM metallolyase superfamily. DRE-TIM metallolyases include 2-isopropylmalate synthase (IPMS), alpha-isopropylmalate synthase (LeuA), 3-hydroxy-3-methylglutaryl-CoA lyase, homocitrate synthase, citramalate synthase, 4-hydroxy-2-oxovalerate aldolase, re-citrate synthase, transcarboxylase 5S, pyruvate carboxylase, AksA, and FrbC. These members all share a conserved triose-phosphate isomerase (TIM) barrel domain consisting of a core beta(8)-alpha(8) motif with the eight parallel beta strands forming an enclosed barrel surrounded by eight alpha helices. The domain has a catalytic center containing a divalent cation-binding site formed by a cluster of invariant residues that cap the core of the barrel. In addition, the catalytic site includes three invariant residues - an aspartate (D), an arginine (R), and a glutamate (E) - which is the basis for the domain name "DRE-TIM". Pssm-ID: 163676 Cd Length: 274 Bit Score: 320.49 E-value: 2.03e-109
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HMGL-like | pfam00682 | HMGL-like; This family contains a diverse set of enzymes. These include various aldolases and ... |
168-349 | 5.96e-24 | ||||
HMGL-like; This family contains a diverse set of enzymes. These include various aldolases and a region of pyruvate carboxylase. Pssm-ID: 459902 [Multi-domain] Cd Length: 264 Bit Score: 98.95 E-value: 5.96e-24
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Name | Accession | Description | Interval | E-value | ||||
PLN02746 | PLN02746 | hydroxymethylglutaryl-CoA lyase |
122-350 | 5.47e-151 | ||||
hydroxymethylglutaryl-CoA lyase Pssm-ID: 178347 Cd Length: 347 Bit Score: 428.82 E-value: 5.47e-151
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DRE_TIM_HMGL | cd07938 | 3-hydroxy-3-methylglutaryl-CoA lyase, catalytic TIM barrel domain; ... |
170-347 | 2.03e-109 | ||||
3-hydroxy-3-methylglutaryl-CoA lyase, catalytic TIM barrel domain; 3-hydroxy-3-methylglutaryl-CoA lyase (HMGL) catalyzes the cleavage of HMG-CoA to acetyl-CoA and acetoacetate, one of the terminal steps in ketone body generation and leucine degradation, and is a key enzyme in the pathway that supplies metabolic fuel to extrahepatic tissues. Mutations in HMGL cause a human autosomal recessive disorder called primary metabolic aciduria that affects ketogenesis and leucine catabolism and can be fatal due to an inability to tolerate hypoglycemia. HMGL has a TIM barrel domain with a catalytic center containing a divalent cation-binding site formed by a cluster of invariant residues that cap the core of the barrel. The cleavage of HMG-CoA requires the presence of a divalent cation like Mg2+ or Mn2+, and the reaction is thought to involve general acid/base catalysis. This family belongs to the DRE-TIM metallolyase superfamily. DRE-TIM metallolyases include 2-isopropylmalate synthase (IPMS), alpha-isopropylmalate synthase (LeuA), 3-hydroxy-3-methylglutaryl-CoA lyase, homocitrate synthase, citramalate synthase, 4-hydroxy-2-oxovalerate aldolase, re-citrate synthase, transcarboxylase 5S, pyruvate carboxylase, AksA, and FrbC. These members all share a conserved triose-phosphate isomerase (TIM) barrel domain consisting of a core beta(8)-alpha(8) motif with the eight parallel beta strands forming an enclosed barrel surrounded by eight alpha helices. The domain has a catalytic center containing a divalent cation-binding site formed by a cluster of invariant residues that cap the core of the barrel. In addition, the catalytic site includes three invariant residues - an aspartate (D), an arginine (R), and a glutamate (E) - which is the basis for the domain name "DRE-TIM". Pssm-ID: 163676 Cd Length: 274 Bit Score: 320.49 E-value: 2.03e-109
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PRK05692 | PRK05692 | hydroxymethylglutaryl-CoA lyase; Provisional |
164-347 | 5.59e-106 | ||||
hydroxymethylglutaryl-CoA lyase; Provisional Pssm-ID: 180206 Cd Length: 287 Bit Score: 312.21 E-value: 5.59e-106
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DRE_TIM_metallolyase | cd03174 | DRE-TIM metallolyase superfamily; The DRE-TIM metallolyase superfamily includes ... |
171-346 | 1.02e-60 | ||||
DRE-TIM metallolyase superfamily; The DRE-TIM metallolyase superfamily includes 2-isopropylmalate synthase (IPMS), alpha-isopropylmalate synthase (LeuA), 3-hydroxy-3-methylglutaryl-CoA lyase, homocitrate synthase, citramalate synthase, 4-hydroxy-2-oxovalerate aldolase, re-citrate synthase, transcarboxylase 5S, pyruvate carboxylase, AksA, and FrbC. These members all share a conserved triose-phosphate isomerase (TIM) barrel domain consisting of a core beta(8)-alpha(8) motif with the eight parallel beta strands forming an enclosed barrel surrounded by eight alpha helices. The domain has a catalytic center containing a divalent cation-binding site formed by a cluster of invariant residues that cap the core of the barrel. In addition, the catalytic site includes three invariant residues - an aspartate (D), an arginine (R), and a glutamate (E) - which is the basis for the domain name "DRE-TIM". Pssm-ID: 163674 [Multi-domain] Cd Length: 265 Bit Score: 195.75 E-value: 1.02e-60
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HMGL-like | pfam00682 | HMGL-like; This family contains a diverse set of enzymes. These include various aldolases and ... |
168-349 | 5.96e-24 | ||||
HMGL-like; This family contains a diverse set of enzymes. These include various aldolases and a region of pyruvate carboxylase. Pssm-ID: 459902 [Multi-domain] Cd Length: 264 Bit Score: 98.95 E-value: 5.96e-24
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Blast search parameters | ||||
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