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Conserved domains on  [gi|281364287|ref|NP_608700|]
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TBP-related factor 5 [Drosophila melanogaster]

Protein Classification

TBP family protein( domain architecture ID 13220299)

TBP family protein similar to TATA-box-binding protein (TBP), a general transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TBP pfam00352
Transcription factor TFIID (or TATA-binding protein, TBP);
189-268 1.12e-17

Transcription factor TFIID (or TATA-binding protein, TBP);


:

Pssm-ID: 425630 [Multi-domain]  Cd Length: 84  Bit Score: 76.32  E-value: 1.12e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287  189 NAVnATFSVPFNLNLRQFHLENPVVTRYDTSKYPFLVYKMMGTTVEIAIFPTGYVIVLFATTMEITKLAIAHILPTLYRL 268
Cdd:pfam00352   6 NVV-ASADLGFPIDLEKLALKLLENVEYEPERFPGLVYRLREPKVTALIFSSGKIVITGAKSEEDAKKAIRKIAPILQKL 84
PLN00062 super family cl28966
TATA-box-binding protein; Provisional
 110-265 3.24e-08

TATA-box-binding protein; Provisional


The actual alignment was detected with superfamily member PLN00062:

Pssm-ID: 177693 [Multi-domain]  Cd Length: 179  Bit Score: 52.39  E-value: 3.24e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287 110 SKFHLLELDMGLKNTAFEPEKHMALFVYRYSPTRSIRIYPHGNIYC-----QAFCKNSARyGLANILkelRYLGYAPRLR 184
Cdd:PLN00062  15 CKLDLKKIALQARNAEYNPKRFAAVIMRIREPKTTALIFASGKMVCtgaksEHDSKLAAR-KYARII---QKLGFPAKFK 90

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287 185 RLKTNAVNATFSVPFNLNLRQFHLENPVVTRYDTSKYPFLVYKMMGTTVEIAIFPTGYVIVLFATTMEITKLAIAHILPT 264
Cdd:PLN00062  91 DFKIQNIVGSCDVKFPIRLEGLAYAHGAFSSYEPELFPGLIYRMKQPKIVLLIFVSGKIVITGAKVREEIYTAFENIYPV 170


                 .
gi 281364287 265 L 265
Cdd:PLN00062 171 L 171
 
Name Accession Description Interval E-value
TBP pfam00352
Transcription factor TFIID (or TATA-binding protein, TBP);
189-268 1.12e-17

Transcription factor TFIID (or TATA-binding protein, TBP);


Pssm-ID: 425630 [Multi-domain]  Cd Length: 84  Bit Score: 76.32  E-value: 1.12e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287  189 NAVnATFSVPFNLNLRQFHLENPVVTRYDTSKYPFLVYKMMGTTVEIAIFPTGYVIVLFATTMEITKLAIAHILPTLYRL 268
Cdd:pfam00352   6 NVV-ASADLGFPIDLEKLALKLLENVEYEPERFPGLVYRLREPKVTALIFSSGKIVITGAKSEEDAKKAIRKIAPILQKL 84
TLF cd04517
TBP-like factors (TLF; also called TLP, TRF, TRP), which are found in most metazoans. TLFs and ...
 114-265 8.79e-14

TBP-like factors (TLF; also called TLP, TRF, TRP), which are found in most metazoans. TLFs and TBPs have well-conserved core domains; however, they only share about 60% similarity. TLFs, like TBPs, interact with TFIIA and TFIIB, which are part of the basal transcription machinery. Yet, in contrast to TBPs, TLFs seem not to interact with the TATA-box and even have a negative effect on the transcription of TATA-containing promoters. Recent results indicate that TLFs are involved in the transcription via TATA-less promoters.


Pssm-ID: 239953  Cd Length: 174  Bit Score: 68.13  E-value: 8.79e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287 114 LLELDMGLKNTAFEPEKHMALFVYRySPTRSIRIYPHGNIYC-----QAFCKNSARyGLANILKELryLGYAPRLRRLKT 188
Cdd:cd04517   20 LRKLALAGRNVEYNPRYPKVTMRLR-EPRATASVWSSGKITItgatsEEEAKQAAR-RAARLLQKL--GFKVVRFSNFRV 95

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 281364287 189 NAVNATFSVPFNLNLRQFHLENPVVTRYDTSKYPFLVYKMMGTTVEIAIFPTGYVIVLFATTMEITKLAIAHILPTL 265
Cdd:cd04517   96 VNVLATCSMPFPIRLDELAAKNRSSASYEPELHPGVVYRITGPRATLSIFSTGSVTVTGARSMEDVREAVEKIYPIV 172
PLN00062 PLN00062
TATA-box-binding protein; Provisional
 110-265 3.24e-08

TATA-box-binding protein; Provisional


Pssm-ID: 177693 [Multi-domain]  Cd Length: 179  Bit Score: 52.39  E-value: 3.24e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287 110 SKFHLLELDMGLKNTAFEPEKHMALFVYRYSPTRSIRIYPHGNIYC-----QAFCKNSARyGLANILkelRYLGYAPRLR 184
Cdd:PLN00062  15 CKLDLKKIALQARNAEYNPKRFAAVIMRIREPKTTALIFASGKMVCtgaksEHDSKLAAR-KYARII---QKLGFPAKFK 90

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287 185 RLKTNAVNATFSVPFNLNLRQFHLENPVVTRYDTSKYPFLVYKMMGTTVEIAIFPTGYVIVLFATTMEITKLAIAHILPT 264
Cdd:PLN00062  91 DFKIQNIVGSCDVKFPIRLEGLAYAHGAFSSYEPELFPGLIYRMKQPKIVLLIFVSGKIVITGAKVREEIYTAFENIYPV 170


                 .
gi 281364287 265 L 265
Cdd:PLN00062 171 L 171
SPT15 COG2101
TATA-box binding protein (TBP), component of TFIID and TFIIIB [Transcription];
114-268 6.51e-05

TATA-box binding protein (TBP), component of TFIID and TFIIIB [Transcription];


Pssm-ID: 441704  Cd Length: 180  Bit Score: 42.88  E-value: 6.51e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287 114 LLELDMGLKNTAFEPEKHMALfVYRY-SPTRSIRIYPHGNIYCQ-AFCKNSARYGLANILKELRYLG----YAPRLRrlK 187
Cdd:COG2101   21 LEKLAEDLPNAEYNPEQFPGL-VYRLkEPKTAALIFRSGKMVCTgAKSEEDLIEAVEKVIEKLRKLGieipDEPEIT--V 97

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287 188 TNAV-NATFSVPFNLNL--RQFHLENpvvTRYDTSKYPFLVYKMMGTTVEIAIFPTGYVIVLFATTMEITKLAIAHILPT 264
Cdd:COG2101   98 QNIVaSADLGREINLNAlaIGLGLEN---IEYEPEQFPGLVYRLDDPKVVLLLFSSGKLVITGAKDEEEAERAVDKLRER 174


                 ....
gi 281364287 265 LYRL 268
Cdd:COG2101  175 LEEL 178
 
Name Accession Description Interval E-value
TBP pfam00352
Transcription factor TFIID (or TATA-binding protein, TBP);
189-268 1.12e-17

Transcription factor TFIID (or TATA-binding protein, TBP);


Pssm-ID: 425630 [Multi-domain]  Cd Length: 84  Bit Score: 76.32  E-value: 1.12e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287  189 NAVnATFSVPFNLNLRQFHLENPVVTRYDTSKYPFLVYKMMGTTVEIAIFPTGYVIVLFATTMEITKLAIAHILPTLYRL 268
Cdd:pfam00352   6 NVV-ASADLGFPIDLEKLALKLLENVEYEPERFPGLVYRLREPKVTALIFSSGKIVITGAKSEEDAKKAIRKIAPILQKL 84
TLF cd04517
TBP-like factors (TLF; also called TLP, TRF, TRP), which are found in most metazoans. TLFs and ...
 114-265 8.79e-14

TBP-like factors (TLF; also called TLP, TRF, TRP), which are found in most metazoans. TLFs and TBPs have well-conserved core domains; however, they only share about 60% similarity. TLFs, like TBPs, interact with TFIIA and TFIIB, which are part of the basal transcription machinery. Yet, in contrast to TBPs, TLFs seem not to interact with the TATA-box and even have a negative effect on the transcription of TATA-containing promoters. Recent results indicate that TLFs are involved in the transcription via TATA-less promoters.


Pssm-ID: 239953  Cd Length: 174  Bit Score: 68.13  E-value: 8.79e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287 114 LLELDMGLKNTAFEPEKHMALFVYRySPTRSIRIYPHGNIYC-----QAFCKNSARyGLANILKELryLGYAPRLRRLKT 188
Cdd:cd04517   20 LRKLALAGRNVEYNPRYPKVTMRLR-EPRATASVWSSGKITItgatsEEEAKQAAR-RAARLLQKL--GFKVVRFSNFRV 95

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 281364287 189 NAVNATFSVPFNLNLRQFHLENPVVTRYDTSKYPFLVYKMMGTTVEIAIFPTGYVIVLFATTMEITKLAIAHILPTL 265
Cdd:cd04517   96 VNVLATCSMPFPIRLDELAAKNRSSASYEPELHPGVVYRITGPRATLSIFSTGSVTVTGARSMEDVREAVEKIYPIV 172
TBP_TLF cd00652
TATA box binding protein (TBP): Present in archaea and eukaryotes, TBPs are transcription ...
 114-265 2.20e-13

TATA box binding protein (TBP): Present in archaea and eukaryotes, TBPs are transcription factors that recognize promoters and initiate transcription. TBP has been shown to be an essential component of three different transcription initiation complexes: SL1, TFIID and TFIIIB, directing transcription by RNA polymerases I, II and III, respectively. TBP binds directly to the TATA box promoter element, where it nucleates polymerase assembly, thus defining the transcription start site. TBP's binding in the minor groove induces a dramatic DNA bending while its own structure barely changes. The conserved core domain of TBP, which binds to the TATA box, has a bipartite structure, with intramolecular symmetry generating a saddle-shaped structure that sits astride the DNA. New members of the TBP family, called TBP-like proteins (TBLP, TLF, TLP) or TBP-related factors (TRF1, TRF2,TRP), are similar to the core domain of TBPs, with identical or chemically similar amino acids at many equivalent positions, suggesting similar structure. However, TLFs contain distinct, conserved amino acids at several positions that distinguish them from TBP.


Pssm-ID: 238352 [Multi-domain]  Cd Length: 174  Bit Score: 66.80  E-value: 2.20e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287 114 LLELDMGLKNTAFEPEKHMALFVYRYSPTRSIRIYPHGNIYCQ-AFCKNSARYGLANILKELRYLGY-APRLRRLKTNAV 191
Cdd:cd00652   19 LRKIALAARNAEYNPKRFPGVIMRLREPKTTALIFSSGKMVITgAKSEEDAKLAARKYARILQKLGFpVEKFPEFKVQNI 98

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 281364287 192 NATFSVPFNLNLRQFHLENPVVTRYDTSKYPFLVYKMMGTTVEIAIFPTGYVIVLFATTMEITKLAIAHILPTL 265
Cdd:cd00652   99 VASCDLGFPIRLEELALKHPENASYEPELFPGLIYRMDEPKVVLLIFVSGKIVITGAKSREDIYEAVEKIYPIL 172
PLN00062 PLN00062
TATA-box-binding protein; Provisional
 110-265 3.24e-08

TATA-box-binding protein; Provisional


Pssm-ID: 177693 [Multi-domain]  Cd Length: 179  Bit Score: 52.39  E-value: 3.24e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287 110 SKFHLLELDMGLKNTAFEPEKHMALFVYRYSPTRSIRIYPHGNIYC-----QAFCKNSARyGLANILkelRYLGYAPRLR 184
Cdd:PLN00062  15 CKLDLKKIALQARNAEYNPKRFAAVIMRIREPKTTALIFASGKMVCtgaksEHDSKLAAR-KYARII---QKLGFPAKFK 90

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287 185 RLKTNAVNATFSVPFNLNLRQFHLENPVVTRYDTSKYPFLVYKMMGTTVEIAIFPTGYVIVLFATTMEITKLAIAHILPT 264
Cdd:PLN00062  91 DFKIQNIVGSCDVKFPIRLEGLAYAHGAFSSYEPELFPGLIYRMKQPKIVLLIFVSGKIVITGAKVREEIYTAFENIYPV 170


                 .
gi 281364287 265 L 265
Cdd:PLN00062 171 L 171
SPT15 COG2101
TATA-box binding protein (TBP), component of TFIID and TFIIIB [Transcription];
114-268 6.51e-05

TATA-box binding protein (TBP), component of TFIID and TFIIIB [Transcription];


Pssm-ID: 441704  Cd Length: 180  Bit Score: 42.88  E-value: 6.51e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287 114 LLELDMGLKNTAFEPEKHMALfVYRY-SPTRSIRIYPHGNIYCQ-AFCKNSARYGLANILKELRYLG----YAPRLRrlK 187
Cdd:COG2101   21 LEKLAEDLPNAEYNPEQFPGL-VYRLkEPKTAALIFRSGKMVCTgAKSEEDLIEAVEKVIEKLRKLGieipDEPEIT--V 97

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 281364287 188 TNAV-NATFSVPFNLNL--RQFHLENpvvTRYDTSKYPFLVYKMMGTTVEIAIFPTGYVIVLFATTMEITKLAIAHILPT 264
Cdd:COG2101   98 QNIVaSADLGREINLNAlaIGLGLEN---IEYEPEQFPGLVYRLDDPKVVLLLFSSGKLVITGAKDEEEAERAVDKLRER 174


                 ....
gi 281364287 265 LYRL 268
Cdd:COG2101  175 LEEL 178
TBP_TLF cd00652
TATA box binding protein (TBP): Present in archaea and eukaryotes, TBPs are transcription ...
 185-259 7.68e-03

TATA box binding protein (TBP): Present in archaea and eukaryotes, TBPs are transcription factors that recognize promoters and initiate transcription. TBP has been shown to be an essential component of three different transcription initiation complexes: SL1, TFIID and TFIIIB, directing transcription by RNA polymerases I, II and III, respectively. TBP binds directly to the TATA box promoter element, where it nucleates polymerase assembly, thus defining the transcription start site. TBP's binding in the minor groove induces a dramatic DNA bending while its own structure barely changes. The conserved core domain of TBP, which binds to the TATA box, has a bipartite structure, with intramolecular symmetry generating a saddle-shaped structure that sits astride the DNA. New members of the TBP family, called TBP-like proteins (TBLP, TLF, TLP) or TBP-related factors (TRF1, TRF2,TRP), are similar to the core domain of TBPs, with identical or chemically similar amino acids at many equivalent positions, suggesting similar structure. However, TLFs contain distinct, conserved amino acids at several positions that distinguish them from TBP.


Pssm-ID: 238352 [Multi-domain]  Cd Length: 174  Bit Score: 36.76  E-value: 7.68e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 281364287 185 RLKTNAVNATFSVPFNLNLRQ--FHLENpvvTRYDTSKYPFLVYKMMGTTVEIAIFPTGYVIVLFATTMEITKLAIA 259
Cdd:cd00652    1 SPKIQNIVATVNLGCELDLRKiaLAARN---AEYNPKRFPGVIMRLREPKTTALIFSSGKMVITGAKSEEDAKLAAR 74
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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