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Conserved domains on  [gi|18416749|ref|NP_567746|]
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COP9 signalosome subunit 6B [Arabidopsis thaliana]

Protein Classification

COP9 signalosome complex subunit 6( domain architecture ID 10169151)

COP9 signalosome complex subunit 6 is a component of the COP9 signalosome complex (CSN), an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes

Gene Ontology:  GO:0000338|GO:0008180|GO:0045116|GO:2000434
MEROPS:  M67
PubMed:  22575649|18926707

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MPN_CSN6 cd08063
Mpr1p, Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity, found in COP9 ...
 9-310 5.66e-143

Mpr1p, Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity, found in COP9 signalosome complex subunit 6; CSN6 (COP9 signalosome subunit 6; COP9 subunit 6; MOV34 homolog, 34 kD) is one of the eight subunits of COP9 signalosome, a highly conserved protein complex with diverse functions, including several important intracellular pathways such as the ubiquitin/proteasome system, DNA repair, cell cycle, developmental changes, and some aspects of immune responses. CSN6 is an MPN-domain protein that directly interacts with the MPN+-domain subunit CSN5. It is cleaved during apoptosis by activated caspases. CSN6 processing occurs in CSN/CRL (cullin-RING Ub ligase) complexes and is followed by the cleavage of Rbx1, the direct interaction partner of CSN6. CSN6 cleavage enhances CSN-mediated deneddylating activity (i.e. cleavage of ubiquitin-like protein Nedd8 (neural precursor cell expressed, developmentally downregulated 8)) in the cullin 1 in cells. The cleavage of Rbx1 and increased deneddylation of cullins inactivate CRLs and presumably stabilize pro-apoptotic factors for final apoptotic steps. While CSN6 shows a typical MPN metalloprotease fold, it lacks the canonical JAMM motif, and therefore does not show catalytic isopeptidase activity.


:

Pssm-ID: 163694 [Multi-domain]  Cd Length: 288  Bit Score: 404.71  E-value: 5.66e-143
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749   9 LTFKLHPLVMLNISDHFTRVKTQLNPPaascatgngsnnadamllqNPRVYGCVIGLQRGRTVEIFNSFELIFDPALD-- 86
Cdd:cd08063   1 LSVKLHPLVILNISDHITRHRAQSQSE-------------------PPRVVGALLGQQDGREIEIENSFELKYDTNEDge 61

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749  87 -TLDRSFLEKKQELYKKVFPDFYVLGWYSTGSD-ATESDMHIHKALMDINESPVYVLLNPAINHAQKDLPVTIYESEFHV 164
Cdd:cd08063  62 iVLDKEFLETRLEQFKQVFKDLDFVGWYTTGPGgPTESDLPIHKQILEINESPVLLLLDPEANASGKDLPVTIYESVLEL 141

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749 165 IDGIPQSIFVHTSYTIETVEAERISVDHVAHLKPSdGGSAATQLAAHLTGIHSAIKMLNSRIRVLYQHIVAMQKGDKPCE 244
Cdd:cd08063 142 VDGEATLRFRELPYTIETGEAERIGVDHVARGGAS-GSSEKSTVAAHLQAQHNAIKMLNSRVELILEYLKAVPVGEVPPD 220

                       250       260       270       280       290       300
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 18416749 245 NSVLRQVSSLLRSLPAAESEKFNENFLMEYNDKLLMSYLAMITNCTSNMNEVVDKFNTAYD--KHSRR 310
Cdd:cd08063 221 HSILRSISALCSRLPVLKSEAFREELLAEYNDVLLVAYLATLTKGCNTLNELVDKFNVVYDrkGSGRR 288
 
Name Accession Description Interval E-value
MPN_CSN6 cd08063
Mpr1p, Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity, found in COP9 ...
 9-310 5.66e-143

Mpr1p, Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity, found in COP9 signalosome complex subunit 6; CSN6 (COP9 signalosome subunit 6; COP9 subunit 6; MOV34 homolog, 34 kD) is one of the eight subunits of COP9 signalosome, a highly conserved protein complex with diverse functions, including several important intracellular pathways such as the ubiquitin/proteasome system, DNA repair, cell cycle, developmental changes, and some aspects of immune responses. CSN6 is an MPN-domain protein that directly interacts with the MPN+-domain subunit CSN5. It is cleaved during apoptosis by activated caspases. CSN6 processing occurs in CSN/CRL (cullin-RING Ub ligase) complexes and is followed by the cleavage of Rbx1, the direct interaction partner of CSN6. CSN6 cleavage enhances CSN-mediated deneddylating activity (i.e. cleavage of ubiquitin-like protein Nedd8 (neural precursor cell expressed, developmentally downregulated 8)) in the cullin 1 in cells. The cleavage of Rbx1 and increased deneddylation of cullins inactivate CRLs and presumably stabilize pro-apoptotic factors for final apoptotic steps. While CSN6 shows a typical MPN metalloprotease fold, it lacks the canonical JAMM motif, and therefore does not show catalytic isopeptidase activity.


Pssm-ID: 163694 [Multi-domain]  Cd Length: 288  Bit Score: 404.71  E-value: 5.66e-143
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749   9 LTFKLHPLVMLNISDHFTRVKTQLNPPaascatgngsnnadamllqNPRVYGCVIGLQRGRTVEIFNSFELIFDPALD-- 86
Cdd:cd08063   1 LSVKLHPLVILNISDHITRHRAQSQSE-------------------PPRVVGALLGQQDGREIEIENSFELKYDTNEDge 61

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749  87 -TLDRSFLEKKQELYKKVFPDFYVLGWYSTGSD-ATESDMHIHKALMDINESPVYVLLNPAINHAQKDLPVTIYESEFHV 164
Cdd:cd08063  62 iVLDKEFLETRLEQFKQVFKDLDFVGWYTTGPGgPTESDLPIHKQILEINESPVLLLLDPEANASGKDLPVTIYESVLEL 141

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749 165 IDGIPQSIFVHTSYTIETVEAERISVDHVAHLKPSdGGSAATQLAAHLTGIHSAIKMLNSRIRVLYQHIVAMQKGDKPCE 244
Cdd:cd08063 142 VDGEATLRFRELPYTIETGEAERIGVDHVARGGAS-GSSEKSTVAAHLQAQHNAIKMLNSRVELILEYLKAVPVGEVPPD 220

                       250       260       270       280       290       300
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 18416749 245 NSVLRQVSSLLRSLPAAESEKFNENFLMEYNDKLLMSYLAMITNCTSNMNEVVDKFNTAYD--KHSRR 310
Cdd:cd08063 221 HSILRSISALCSRLPVLKSEAFREELLAEYNDVLLVAYLATLTKGCNTLNELVDKFNVVYDrkGSGRR 288
PLN03246 PLN03246
26S proteasome regulatory subunit; Provisional
 13-289 6.56e-29

26S proteasome regulatory subunit; Provisional


Pssm-ID: 215645 [Multi-domain]  Cd Length: 303  Bit Score: 112.53  E-value: 6.56e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749   13 LHPLVMLNISDHFTRVktqlnppaascATGNgsnnadamllqNPRVYGCVIGLQRGRTVEIFNSFELIF-----DPALDT 87
Cdd:PLN03246  10 VHPLVLLSIVDHYNRV-----------AKDT-----------RKRVVGVLLGSSFRGRVDVTNSFAVPFeeddkDPSIWF 67

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749   88 LDRSFLEKKQELYKKVFPDFYVLGWYSTGSDATESDMHIHKALMDINESPVYVLLNpaINHAQKDLPVTIYESEFHVIDG 167
Cdd:PLN03246  68 LDHNYLESMFGMFKRINAKEHVVGWYSTGPKLRENDLDIHELFNDYVPNPVLVIID--VQPKELGIPTKAYYAVEEVKEN 145

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749  168 IPQS---IFVHTSYTIETVEAERISVDHVAH-LKPSDGGSAATQLAAHLTgihsAIKMLNSRIRVLYQHIVAMQKGDKPC 243
Cdd:PLN03246 146 ATQKsqkVFVHVPSEIGAHEAEEIGVEHLLRdVKDTTVSTLATEVTGKLT----ALKGLDARLREIRSYLDLVVEGKLPL 221

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*.
gi 18416749  244 ENSVLRQVSSLLRSLPAAESEKFNENFLMEYNDKLLMSYLAMITNC 289
Cdd:PLN03246 222 NHEILYHLQDVFNLLPNLNVEELVKAFAVKTNDMMLVIYLSSLIRS 267
JAB pfam01398
JAB1/Mov34/MPN/PAD-1 ubiquitin protease; Members of this family are found in proteasome ...
 6-136 7.26e-27

JAB1/Mov34/MPN/PAD-1 ubiquitin protease; Members of this family are found in proteasome regulatory subunits, eukaryotic initiation factor 3 (eIF3) subunits and regulators of transcription factors. This family is also known as the MPN domain and PAD-1-like domain, JABP1 domain or JAMM domain. These are metalloenzymes that function as the ubiquitin isopeptidase/ deubiquitinase in the ubiquitin-based signalling and protein turnover pathways in eukaryotes. Versions of the domain in prokaryotic cognates of the ubiquitin-modification pathway are shown to have a similar role, and the archael protein from Haloferax volcanii is found to cleave ubiquitin-like small archaeal modifier proteins (SAMP1/2) from protein conjugates.


Pssm-ID: 396120  Cd Length: 117  Bit Score: 102.04  E-value: 7.26e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749     6 SSGLTFKLHPLVMLNISDHFTRVKTQlnppaascatgngsnnadamllqNPRVYGCVIGLQRGR-TVEIFNSFELIFDPA 84
Cdd:pfam01398   1 SSVRTVIIHPLVLLKILDHANRGGKI-----------------------GEEVMGVLLGKLEGDgTIEITNSFALPQEET 57

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749    85 LDT-----LDRSFLEKKQELYKKVFPDFYVLGWYSTGSDA---TESDMHIHKALMDINES 136
Cdd:pfam01398  58 EDDvnavaLDQEYMENMHEMLKKVNRKEEVVGWYHTHPGLcwlSSVDVHTHALYQRMIPE 117
JAB_MPN smart00232
JAB/MPN domain; Domain in Jun kinase activation domain binding protein and proteasomal ...
 10-160 3.24e-11

JAB/MPN domain; Domain in Jun kinase activation domain binding protein and proteasomal subunits. Domain at Mpr1p and Pad1p N-termini. Domain of unknown function.


Pssm-ID: 214573  Cd Length: 135  Bit Score: 60.08  E-value: 3.24e-11
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749     10 TFKLHPLVMLNISDHFTRvktqlnppaascatgnGSNNadamllqnpRVYGCVIGLQRGRTVEIFNSFELIFDPALDTLD 89
Cdd:smart00232   1 EVKVHPLVPLNILKHAIR----------------DGPE---------EVCGVLLGKSNKDRPEVKEVFAVPNEPQDDSVQ 55

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 18416749     90 RSFLEKKQ---ELYKKVFPDFYVLGWYSTGSDA----TESDMHIHKALMDINESPVYVLLNPaINHAQKDLPVTIYES 160
Cdd:smart00232  56 EYDEDYSHlmdEELKKVNKDLEIVGWYHSHPDEspfpSEVDVATHESYQAPWPISVVLIVDP-IKSFQGRLSLRAFRL 132
 
Name Accession Description Interval E-value
MPN_CSN6 cd08063
Mpr1p, Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity, found in COP9 ...
 9-310 5.66e-143

Mpr1p, Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity, found in COP9 signalosome complex subunit 6; CSN6 (COP9 signalosome subunit 6; COP9 subunit 6; MOV34 homolog, 34 kD) is one of the eight subunits of COP9 signalosome, a highly conserved protein complex with diverse functions, including several important intracellular pathways such as the ubiquitin/proteasome system, DNA repair, cell cycle, developmental changes, and some aspects of immune responses. CSN6 is an MPN-domain protein that directly interacts with the MPN+-domain subunit CSN5. It is cleaved during apoptosis by activated caspases. CSN6 processing occurs in CSN/CRL (cullin-RING Ub ligase) complexes and is followed by the cleavage of Rbx1, the direct interaction partner of CSN6. CSN6 cleavage enhances CSN-mediated deneddylating activity (i.e. cleavage of ubiquitin-like protein Nedd8 (neural precursor cell expressed, developmentally downregulated 8)) in the cullin 1 in cells. The cleavage of Rbx1 and increased deneddylation of cullins inactivate CRLs and presumably stabilize pro-apoptotic factors for final apoptotic steps. While CSN6 shows a typical MPN metalloprotease fold, it lacks the canonical JAMM motif, and therefore does not show catalytic isopeptidase activity.


Pssm-ID: 163694 [Multi-domain]  Cd Length: 288  Bit Score: 404.71  E-value: 5.66e-143
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749   9 LTFKLHPLVMLNISDHFTRVKTQLNPPaascatgngsnnadamllqNPRVYGCVIGLQRGRTVEIFNSFELIFDPALD-- 86
Cdd:cd08063   1 LSVKLHPLVILNISDHITRHRAQSQSE-------------------PPRVVGALLGQQDGREIEIENSFELKYDTNEDge 61

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749  87 -TLDRSFLEKKQELYKKVFPDFYVLGWYSTGSD-ATESDMHIHKALMDINESPVYVLLNPAINHAQKDLPVTIYESEFHV 164
Cdd:cd08063  62 iVLDKEFLETRLEQFKQVFKDLDFVGWYTTGPGgPTESDLPIHKQILEINESPVLLLLDPEANASGKDLPVTIYESVLEL 141

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749 165 IDGIPQSIFVHTSYTIETVEAERISVDHVAHLKPSdGGSAATQLAAHLTGIHSAIKMLNSRIRVLYQHIVAMQKGDKPCE 244
Cdd:cd08063 142 VDGEATLRFRELPYTIETGEAERIGVDHVARGGAS-GSSEKSTVAAHLQAQHNAIKMLNSRVELILEYLKAVPVGEVPPD 220

                       250       260       270       280       290       300
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 18416749 245 NSVLRQVSSLLRSLPAAESEKFNENFLMEYNDKLLMSYLAMITNCTSNMNEVVDKFNTAYD--KHSRR 310
Cdd:cd08063 221 HSILRSISALCSRLPVLKSEAFREELLAEYNDVLLVAYLATLTKGCNTLNELVDKFNVVYDrkGSGRR 288
MPN_RPN7_8 cd08062
Mpr1p, Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity, found in 19S ...
 13-290 3.65e-32

Mpr1p, Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity, found in 19S proteasomal subunits Rpn7 and Rpn8; This family includes lid subunits of the 26 S proteasome regulatory particles, Rpn7 (PSMD7; proteasome 26S non-ATPase subunit 7; p44), and Rpn8 (PSMD8; proteasome 26S non-ATPase subunit 8; p40; Mov34). Rpn7 is known to be critical for the integrity of the 26 S proteasome complex by establishing a correct lid structure. It is necessary for the incorporation/anchoring of Rpn3 and Rpn12 to the lid and essential for viability and normal mitosis. Rpn7 and Rpn8 are ATP-independent components of the 19S regulator subunit, and contain the MPN structural motif on its N-terminal region. However, while they show a typical MPN metalloprotease fold, they lack the canonical JAMM motif, and therefore do not show catalytic isopeptidase activity. It is suggested that Rpn7 function is primarily structural.


Pssm-ID: 163693 [Multi-domain]  Cd Length: 280  Bit Score: 120.77  E-value: 3.65e-32
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749  13 LHPLVMLNISDHFTRVktqlnppaascATGNgsnnadamllqNPRVYGCVIGLQRGRTVEIFNSFELIF-----DPALDT 87
Cdd:cd08062   5 VHPLVLLSVVDHYNRV-----------AKGT-----------SKRVVGVLLGSWKKGVLDVTNSFAVPFeedekDPSVWF 62

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749  88 LDRSFLEKKQELYKKVFPDFYVLGWYSTGSDATESDMHIHKALMDINESPVYVLLNpaINHAQKDLPVTIYES--EFHVi 165
Cdd:cd08062  63 LDHNYLENMYGMFKKVNAKEKIVGWYSTGPKLRPNDLDINELFRRYCPNPVLVIID--VRPKDLGLPTEAYIAveEVHD- 139

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749 166 DGIP-QSIFVHTSYTIETVEAERISVDHVAH-LKPSDGGSAATQLAAHLTGihsaIKMLNSRIRVLYQHIVAMQKGDKPC 243
Cdd:cd08062 140 DGTPtSKTFVHVPSEIGAEEAEEVGVEHLLRdIKDVTVSTLSTRVTNKLNS----LKGLQSKLKEIKDYLQLVVEGKLPI 215

                       250       260       270       280
                ....*....|....*....|....*....|....*....|....*..
gi 18416749 244 ENSVLRQVSSLLRSLPAAESEKFNENFLMEYNDKLLMSYLAMITNCT 290
Cdd:cd08062 216 NHQIIYNLQDIFNLLPNLNLPELVKAFAVKTNDQMLVIYLSSLIRSV 262
MPN_euk_non_mb cd08057
Mpr1p, Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity (non ...
 12-188 2.36e-30

Mpr1p, Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity (non metal-binding); eukaryotic; This family contains MPN (also known as Mov34, PAD-1, JAMM, JAB, MPN+) domains variants lacking key residues in the JAB1/MPN/Mov34 metalloenzyme (JAMM) motif and are unable to coordinate a metal ion. Comparisons of key catalytic and metal binding residues explain why the MPN-containing proteins Rpn7/PSMD7, Rpn8/PSMD8, CSN6, Prp8p, and the translation initiation factor 3 subunits f and h do not show catalytic isopeptidase activity. It has been proposed that the MPN domain in these proteins has a primarily structural function. Rpn7 is known to be critical for the integrity of the 26S proteasome complex by establishing a correct lid structure. It is necessary for the incorporation/anchoring of Rpn3 and Rpn12 to the lid and essential for viability and normal mitosis. CSN6 is a highly conserved protein complex with diverse functions, including several important intracellular pathways such as the ubiquitin/proteasome system, DNA repair, cell cycle, developmental changes, and some aspects of immune responses. It cleaves ubiquitin-like protein Nedd8 (neural precursor cell expressed, developmentally downregulated 8)) in the cullin 1 in cells. EIF3f s a potent inhibitor of HIV-1 replication as well as an important negative regulator of cell growth and proliferation. EIF3h regulates cell growth and viability, and that over-expression of the gene may provide growth advantage to prostate, breast, and liver cancer cells.


Pssm-ID: 163688 [Multi-domain]  Cd Length: 157  Bit Score: 112.54  E-value: 2.36e-30
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749  12 KLHPLVMLNISDHFTRVKTQlnppaascatgngsnnadamllqNPRVYGCVIGLQRGRTVEIFNSFELIFDP--ALDTLD 89
Cdd:cd08057   2 QLHPLVLLNISDHYTRRKYG-----------------------IKRVIGVLLGYVDGDKIEVTNSFELPFDEeeESIFID 58

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749  90 RSFLEKKQELYKKVFPDFYVLGWYSTGSDA----TESDMHIHKALMDINE-SPVYVLLNPAINHAQKDLPVTIYESEfhv 164
Cdd:cd08057  59 TEYLEKRYNLHKKVYPQEKIVGWYSIGSNNsneiSKSDNSLHSQFSLISEeNPLILILDPSLQSDSEKLEISTFTSA--- 135

                       170       180
                ....*....|....*....|....
gi 18416749 165 idgIPQSIFVHTSYTIETVEAERI 188
Cdd:cd08057 136 ---QREENGAEITYEIGTEETERI 156
PLN03246 PLN03246
26S proteasome regulatory subunit; Provisional
 13-289 6.56e-29

26S proteasome regulatory subunit; Provisional


Pssm-ID: 215645 [Multi-domain]  Cd Length: 303  Bit Score: 112.53  E-value: 6.56e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749   13 LHPLVMLNISDHFTRVktqlnppaascATGNgsnnadamllqNPRVYGCVIGLQRGRTVEIFNSFELIF-----DPALDT 87
Cdd:PLN03246  10 VHPLVLLSIVDHYNRV-----------AKDT-----------RKRVVGVLLGSSFRGRVDVTNSFAVPFeeddkDPSIWF 67

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749   88 LDRSFLEKKQELYKKVFPDFYVLGWYSTGSDATESDMHIHKALMDINESPVYVLLNpaINHAQKDLPVTIYESEFHVIDG 167
Cdd:PLN03246  68 LDHNYLESMFGMFKRINAKEHVVGWYSTGPKLRENDLDIHELFNDYVPNPVLVIID--VQPKELGIPTKAYYAVEEVKEN 145

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749  168 IPQS---IFVHTSYTIETVEAERISVDHVAH-LKPSDGGSAATQLAAHLTgihsAIKMLNSRIRVLYQHIVAMQKGDKPC 243
Cdd:PLN03246 146 ATQKsqkVFVHVPSEIGAHEAEEIGVEHLLRdVKDTTVSTLATEVTGKLT----ALKGLDARLREIRSYLDLVVEGKLPL 221

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*.
gi 18416749  244 ENSVLRQVSSLLRSLPAAESEKFNENFLMEYNDKLLMSYLAMITNC 289
Cdd:PLN03246 222 NHEILYHLQDVFNLLPNLNVEELVKAFAVKTNDMMLVIYLSSLIRS 267
JAB pfam01398
JAB1/Mov34/MPN/PAD-1 ubiquitin protease; Members of this family are found in proteasome ...
 6-136 7.26e-27

JAB1/Mov34/MPN/PAD-1 ubiquitin protease; Members of this family are found in proteasome regulatory subunits, eukaryotic initiation factor 3 (eIF3) subunits and regulators of transcription factors. This family is also known as the MPN domain and PAD-1-like domain, JABP1 domain or JAMM domain. These are metalloenzymes that function as the ubiquitin isopeptidase/ deubiquitinase in the ubiquitin-based signalling and protein turnover pathways in eukaryotes. Versions of the domain in prokaryotic cognates of the ubiquitin-modification pathway are shown to have a similar role, and the archael protein from Haloferax volcanii is found to cleave ubiquitin-like small archaeal modifier proteins (SAMP1/2) from protein conjugates.


Pssm-ID: 396120  Cd Length: 117  Bit Score: 102.04  E-value: 7.26e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749     6 SSGLTFKLHPLVMLNISDHFTRVKTQlnppaascatgngsnnadamllqNPRVYGCVIGLQRGR-TVEIFNSFELIFDPA 84
Cdd:pfam01398   1 SSVRTVIIHPLVLLKILDHANRGGKI-----------------------GEEVMGVLLGKLEGDgTIEITNSFALPQEET 57

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749    85 LDT-----LDRSFLEKKQELYKKVFPDFYVLGWYSTGSDA---TESDMHIHKALMDINES 136
Cdd:pfam01398  58 EDDvnavaLDQEYMENMHEMLKKVNRKEEVVGWYHTHPGLcwlSSVDVHTHALYQRMIPE 117
MPN_eIF3f cd08064
Mpr1p, Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity, found in eIF3f; ...
 12-288 9.37e-25

Mpr1p, Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity, found in eIF3f; Eukaryotic translation initiation factor 3 (eIF3) subunit F (eIF3F; EIF3S5; eIF3-p47; eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa; Mov34/MPN/PAD-1 family protein) is an evolutionarily non-conserved subunit of the functional core that comprises eIF3a, eIF3b, eIF3c, eIF3e, eIF3f, and eIF3h, and contains the MPN domain. However, it lacks the canonical JAMM motif, and therefore does not show catalytic isopeptidase activity. It has been shown that eIF3f mRNA expression is significantly decreased in many human tumors including pancreatic cancer and melanoma. EIF3f is a potent inhibitor of HIV-1 replication; it mediates restriction of HIV-1 expression through several factors including the serine/arginine-rich (SR) protein 9G8, and cyclin-dependent kinase 11 (CDK11). EIF3f phosphorylation by CDK11 is important in regulating its function in translation and apoptosis. It enhances its association with the core eIF3 subunits during apoptosis, suggesting that eIF3f may inhibit translation by increasing the binding to the eIF3 complex during apoptosis. Thus, eIF3f may be an important negative regulator of cell growth and proliferation.


Pssm-ID: 163695 [Multi-domain]  Cd Length: 265  Bit Score: 100.36  E-value: 9.37e-25
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749  12 KLHPLVMLNISDHFTRvktqlnppaascatgngsNNADAmllqnPRVYGCVIGLQRGRTVEIFNSFElifDPALDT---- 87
Cdd:cd08064   2 RVHPVVLFSILDSYER------------------RNEGQ-----ERVIGTLLGTRSEGEVEITNCFA---VPHNESedqv 55

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749  88 -LDRSFLEKKQELYKKVFPDFYVLGWYSTGSDATESDMHIHKALMDINES--PVYVLLNPAInhAQKDLPVTIYES-EFH 163
Cdd:cd08064  56 aVDMEYHRTMYELHQKVNPKEVIVGWYATGSEITEHSALIHDYYSRECTSynPIHLTVDTSL--DDGKMSIKAYVSsPLG 133

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749 164 VIDGIPQSIFVHTSYTIETVEAERISVDHVAhlKPSDGGSAATQLAAHLTGIHSAIKMLNSRIRVLYQHIVAMQKGDKPC 243
Cdd:cd08064 134 VPGKTLGSMFVPIPLELLYSEAERVALDLLA--KTLASPSRSAPLTSDLEQLEASLEKLQEMLDRVLRYVEDVLAGKVKA 211

                       250       260       270       280
                ....*....|....*....|....*....|....*....|....*
gi 18416749 244 ENSVLRQVSSLLRSLPAAESEKFNENFLMEYNDKLLMSYLAMITN 288
Cdd:cd08064 212 DNAIGRYLMDALTSVPKLDPEEFEKMFNSSLQDLLMVTYLSNLTK 256
MitMem_reg pfam13012
Maintenance of mitochondrial structure and function; This is C-terminal to the Mov24 region of ...
 184-255 4.02e-14

Maintenance of mitochondrial structure and function; This is C-terminal to the Mov24 region of the yeast proteasomal subunit Rpn11 and seems likely to regulate the mitochondrial fission and tubulation processes, ie the outer mitochondrial membrane proteins. This function appears to be unrelated to the proteasome activity of the N-terminal region.


Pssm-ID: 463772 [Multi-domain]  Cd Length: 72  Bit Score: 66.37  E-value: 4.02e-14
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 18416749   184 EAERISVDHVAhlKPSdggsaATQLAAHLTGIHSAIKMLNSRIRVLYQHIVAMQKGDKPCENSVLRQVSSLL 255
Cdd:pfam13012   1 EAERIGVDHLA--RPD-----IKSLSSDLERQYYSLKMLQDRLDLILKYVEDVLDGELPPDHAIGRYLQDLL 65
JAB_MPN smart00232
JAB/MPN domain; Domain in Jun kinase activation domain binding protein and proteasomal ...
 10-160 3.24e-11

JAB/MPN domain; Domain in Jun kinase activation domain binding protein and proteasomal subunits. Domain at Mpr1p and Pad1p N-termini. Domain of unknown function.


Pssm-ID: 214573  Cd Length: 135  Bit Score: 60.08  E-value: 3.24e-11
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18416749     10 TFKLHPLVMLNISDHFTRvktqlnppaascatgnGSNNadamllqnpRVYGCVIGLQRGRTVEIFNSFELIFDPALDTLD 89
Cdd:smart00232   1 EVKVHPLVPLNILKHAIR----------------DGPE---------EVCGVLLGKSNKDRPEVKEVFAVPNEPQDDSVQ 55

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 18416749     90 RSFLEKKQ---ELYKKVFPDFYVLGWYSTGSDA----TESDMHIHKALMDINESPVYVLLNPaINHAQKDLPVTIYES 160
Cdd:smart00232  56 EYDEDYSHlmdEELKKVNKDLEIVGWYHSHPDEspfpSEVDVATHESYQAPWPISVVLIVDP-IKSFQGRLSLRAFRL 132
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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