RhoGAP_MgcRacGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in MgcRacGAP proteins. MgcRacGAP plays an important dual role in cytokinesis: i) it is part of centralspindlin-complex, together with the mitotic kinesin MKLP1, which is critical for the structure of the central spindle by promoting microtuble bundling. ii) after phosphorylation by aurora B MgcRacGAP becomes an effective regulator of RhoA and plays an important role in the assembly of the contractile ring and the initiation of cytokinesis. MgcRacGAP-like proteins contain a N-terminal C1-like domain, and a C-terminal RhoGAP domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 142 IGTKRGClsDYAPRVAPMVPALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRGKSTPHLGNKDTHTLCCCVKD 221
Cdd:cd04382   1 IGTGELA--DFDPSTSPMIPALIVHCVNEIEARGLTEEGLYRVSGSEREVKALKEKFLRGKTVPNLSKVDIHVICGCLKD 78

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 222 FLRQLVHPLIPIYHRRDFEEATRHEDRLAVEMAVYLAVLELHQAHRDTLAYLMLHWQKIAESPAVRMTVNNLAVIFAPTL 301
Cdd:cd04382  79 FLRSLKEPLITFALWKEFMEAAEILDEDNSRAALYQAISELPQPNRDTLAFLILHLQRVAQSPECKMDINNLARVFGPTI 158

                       170       180       190
                ....*....|....*....|....*....|....*
gi 17136438 302 FGDLDL------TLENVVTWQRVLKVLLLMPAGFW 330
Cdd:cd04382 159 VGYSVPnpdpmtILQDTVRQPRVVERLLEIPSDYW 193

RhoGAP_MgcRacGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in MgcRacGAP proteins. MgcRacGAP plays an important dual role in cytokinesis: i) it is part of centralspindlin-complex, together with the mitotic kinesin MKLP1, which is critical for the structure of the central spindle by promoting microtuble bundling. ii) after phosphorylation by aurora B MgcRacGAP becomes an effective regulator of RhoA and plays an important role in the assembly of the contractile ring and the initiation of cytokinesis. MgcRacGAP-like proteins contain a N-terminal C1-like domain, and a C-terminal RhoGAP domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 142 IGTKRGClsDYAPRVAPMVPALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRGKSTPHLGNKDTHTLCCCVKD 221
Cdd:cd04382   1 IGTGELA--DFDPSTSPMIPALIVHCVNEIEARGLTEEGLYRVSGSEREVKALKEKFLRGKTVPNLSKVDIHVICGCLKD 78

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 222 FLRQLVHPLIPIYHRRDFEEATRHEDRLAVEMAVYLAVLELHQAHRDTLAYLMLHWQKIAESPAVRMTVNNLAVIFAPTL 301
Cdd:cd04382  79 FLRSLKEPLITFALWKEFMEAAEILDEDNSRAALYQAISELPQPNRDTLAFLILHLQRVAQSPECKMDINNLARVFGPTI 158

                       170       180       190
                ....*....|....*....|....*....|....*
gi 17136438 302 FGDLDL------TLENVVTWQRVLKVLLLMPAGFW 330
Cdd:cd04382 159 VGYSVPnpdpmtILQDTVRQPRVVERLLEIPSDYW 193

RhoGAP domain; GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438   161 PALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRGKST-PHLGNKDTHTLCCCVKDFLRQLVHPLIPIYHRRDF 239
Cdd:pfam00620   1 PLIVRKCVEYLEKRGLDTEGIFRVSGSASRIKELREAFDRGPDVdLDLEEEDVHVVASLLKLFLRELPEPLLTFELYEEF 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 17136438   240 EEATRHEDRLAVEMAVYLAVLELHQAHRDTLAYLMLHWQKIAESPAV-RMTVNNLAVIFAPTLF 302
Cdd:pfam00620  81 IEAAKLPDEEERLEALRELLRKLPPANRDTLRYLLAHLNRVAQNSDVnKMNAHNLAIVFGPTLL 144

protein kinase C conserved region 1 (C1 domain) found in male germ cell RacGap (MgcRacGAP) and similar proteins; MgcRacGAP, also called Rac GTPase-activating protein 1 (RACGAP1) or protein CYK4, plays an important dual role in cytokinesis: i) it is part of centralspindlin-complex, together with the mitotic kinesin MKLP1, which is critical for the structure of the central spindle by promoting microtuble bundling; and ii) after phosphorylation by aurora B, MgcRacGAP becomes an effective regulator of RhoA and plays an important role in the assembly of the contractile ring and the initiation of cytokinesis. MgcRacGAP-like proteins contain an N-terminal C1 domain, and a C-terminal RhoGAP domain. This model corresponds to the C1 domain. The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.

                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 17136438  85 REHNFKIKSYYYNvGNCVHCRKRIRFAMASLRCRACPLRCHIGCCRQLTVNCIP 138
Cdd:cd20821   1 RPHRFVSKTVIKP-ETCVVCGKRIKFGKKALKCKDCRVVCHPDCKDKLPLPCVP 53

RhoGAP_MgcRacGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in MgcRacGAP proteins. MgcRacGAP plays an important dual role in cytokinesis: i) it is part of centralspindlin-complex, together with the mitotic kinesin MKLP1, which is critical for the structure of the central spindle by promoting microtuble bundling. ii) after phosphorylation by aurora B MgcRacGAP becomes an effective regulator of RhoA and plays an important role in the assembly of the contractile ring and the initiation of cytokinesis. MgcRacGAP-like proteins contain a N-terminal C1-like domain, and a C-terminal RhoGAP domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 142 IGTKRGClsDYAPRVAPMVPALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRGKSTPHLGNKDTHTLCCCVKD 221
Cdd:cd04382   1 IGTGELA--DFDPSTSPMIPALIVHCVNEIEARGLTEEGLYRVSGSEREVKALKEKFLRGKTVPNLSKVDIHVICGCLKD 78

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 222 FLRQLVHPLIPIYHRRDFEEATRHEDRLAVEMAVYLAVLELHQAHRDTLAYLMLHWQKIAESPAVRMTVNNLAVIFAPTL 301
Cdd:cd04382  79 FLRSLKEPLITFALWKEFMEAAEILDEDNSRAALYQAISELPQPNRDTLAFLILHLQRVAQSPECKMDINNLARVFGPTI 158

                       170       180       190
                ....*....|....*....|....*....|....*
gi 17136438 302 FGDLDL------TLENVVTWQRVLKVLLLMPAGFW 330
Cdd:cd04382 159 VGYSVPnpdpmtILQDTVRQPRVVERLLEIPSDYW 193

RhoGAP domain; GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438   161 PALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRGKST-PHLGNKDTHTLCCCVKDFLRQLVHPLIPIYHRRDF 239
Cdd:pfam00620   1 PLIVRKCVEYLEKRGLDTEGIFRVSGSASRIKELREAFDRGPDVdLDLEEEDVHVVASLLKLFLRELPEPLLTFELYEEF 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 17136438   240 EEATRHEDRLAVEMAVYLAVLELHQAHRDTLAYLMLHWQKIAESPAV-RMTVNNLAVIFAPTLF 302
Cdd:pfam00620  81 IEAAKLPDEEERLEALRELLRKLPPANRDTLRYLLAHLNRVAQNSDVnKMNAHNLAIVFGPTLL 144

RhoGAP_fRGD1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of fungal RGD1-like proteins. Yeast Rgd1 is a GAP protein for Rho3 and Rho4 and plays a role in low-pH response. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 149 LSDYAPRVAPMVPALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRGKSTPHLGNK-----DTHTLCCCVKDFL 223
Cdd:cd04398   5 LEDLILREGDNVPNIVYQCIQAIENFGLNLEGIYRLSGNVSRVNKLKELFDKDPLNVLLISPedyesDIHSVASLLKLFF 84

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 224 RQLVHPLIPIYHRRDFEEATRHEDRLAVEMAVYLAVLELHQAHRDTLAYLMLHWQKIAESPAV-RMTVNNLAVIFAPTLF 302
Cdd:cd04398  85 RELPEPLLTKALSREFIEAAKIEDESRRRDALHGLINDLPDANYATLRALMFHLARIKEHESVnRMSVNNLAIIWGPTLM 164

                       170       180
                ....*....|....*....|.
gi 17136438 303 GDLDLTLENVVTWQRVLKVLL 323
Cdd:cd04398 165 NAAPDNAADMSFQSRVIETLL 185

RhoGAP_ARHGAP27_15_12_9: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP27 (also called CAMGAP1), ARHGAP15, 12 and 9-like proteins; This subgroup of ARHGAPs are multidomain proteins that contain RhoGAP, PH, SH3 and WW domains. Most members that are studied show GAP activity towards Rac1, some additionally show activity towards Cdc42. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 147 GC-LSDYAPRVAPMVPALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLR------RKLLRGKStphlGNKDTHTLCCCV 219
Cdd:cd04403   2 GChLEALCQRENSTVPKFVRLCIEAVEKRGLDVDGIYRVSGNLAVIQKLRfavdhdEKLDLDDS----KWEDIHVITGAL 77

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 220 KDFLRQLVHPLIPIYHRRDFEEATRHEDRLAVEMAVYLAVLELHQAHRDTLAYLMLHWQKIAE-SPAVRMTVNNLAVIFA 298
Cdd:cd04403  78 KLFFRELPEPLFPYSLFNDFVAAIKLSDYEQRVSAVKDLIKSLPKPNHDTLKMLFRHLCRVIEhGEKNRMTTQNLAIVFG 157

                ....
gi 17136438 299 PTLF 302
Cdd:cd04403 158 PTLL 161

RhoGAP_GMIP_PARG1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of GMIP (Gem interacting protein) and PARG1 (PTPL1-associated RhoGAP1). GMIP plays important roles in neurite growth and axonal guidance, and interacts with Gem, a member of the RGK subfamily of the Ras small GTPase superfamily, through the N-terminal half of the protein. GMIP contains a C-terminal RhoGAP domain. GMIP inhibits RhoA function, but is inactive towards Rac1 and Cdc41. PARG1 interacts with Rap2, also a member of the Ras small GTPase superfamily whose exact function is unknown, and shows strong preference for Rho. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 153 APRVAP-MVPALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRGKSTPHLGNKDTHTLCCCVKDFLRQLVHPLI 231
Cdd:cd04378   8 VPRDFPdEVPFIIKKCTSEIENRALGVQGIYRVSGSKARVEKLCQAFENGKDLVELSELSPHDISSVLKLFLRQLPEPLI 87

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 232 P-------IYHRRDFEEATRHEDRLAVEMAVYLAVLELHQ-------AHRDTLAYLMLHWQKIAE-SPAVRMTVNNLAVI 296
Cdd:cd04378  88 LfrlyndfIALAKEIQRDTEEDKAPNTPIEVNRIIRKLKDllrqlpaSNYNTLQHLIAHLYRVAEqFEENKMSPNNLGIV 167

                ....*
gi 17136438 297 FAPTL 301
Cdd:cd04378 168 FGPTL 172

RhoGAP_p190: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an N-terminal GTPase domain and a C-terminal GAP domain. The central portion of p190 contains important regulatory phosphorylation sites. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 160 VPALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRGKSTpHLGNKDT--HTLCCCVKDFLRQLVHPLIPIYHRR 237
Cdd:cd04373  15 IPIFLEKCVEFIEATGLETEGIYRVSGNKTHLDSLQKQFDQDHNL-DLVSKDFtvNAVAGALKSFFSELPDPLIPYSMHL 93

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 17136438 238 DFEEATRHEDRLAVEMAVYLAVLELHQAHRDTLAYLMLHWQKIAE-SPAVRMTVNNLAVIFAPTLF 302
Cdd:cd04373  94 ELVEAAKINDREQRLHALKELLKKFPPENFDVFKYVITHLNKVSQnSKVNLMTSENLSICFWPTLM 159

RhoGAP_ARHGAP6: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP6-like proteins. ArhGAP6 shows GAP activity towards RhoA, but not towards Cdc42 and Rac1. ArhGAP6 is often deleted in microphthalmia with linear skin defects syndrome (MLS); MLS is a severe X-linked developmental disorder. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 156 VAPMVPALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRGKSTPHLGNKDTHTLCCCVKDFLRQLVHPLIPIYH 235
Cdd:cd04376   5 IARQVPRLVESCCQHLEKHGLQTVGIFRVGSSKKRVRQLREEFDRGIDVVLDENHSVHDVAALLKEFFRDMPDPLLPREL 84

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 236 RRDFEEAT--RHEDRL-AVEMAVYLavleLHQAHRDTLAYLMLHWQKIAES------------PAVRMTVNNLAVIFAPT 300
Cdd:cd04376  85 YTAFIGTAllEPDEQLeALQLLIYL----LPPCNCDTLHRLLKFLHTVAEHaadsidedgqevSGNKMTSLNLATIFGPN 160

                .
gi 17136438 301 L 301
Cdd:cd04376 161 L 161

RhoGAP_Graf: GTPase-activator protein (GAP) domain for Rho-like GTPases found in GRAF (GTPase regulator associated with focal adhesion kinase); Graf is a multi-domain protein, containing SH3 and PH domains, that binds focal adhesion kinase and influences cytoskeletal changes mediated by Rho proteins. Graf exhibits GAP activity toward RhoA and Cdc42, but only weakly activates Rac1. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 167 CVTEIEARGLQQEGLYRVSSTREKCKRLRRKLL-RGKSTP-----HLGNKDTHTLCCCVKDFLRQLVHPLIPIYHRRDFE 240
Cdd:cd04374  35 CIEAVETRGINEQGLYRVVGVNSKVQKLLSLGLdPKTSTPgdvdlDNSEWEIKTITSALKTYLRNLPEPLMTYELHNDFI 114

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 241 EATRHEDRLAVEMAVYLAVLELHQAHRDTLAYLMLHWQKIAE-SPAVRMTVNNLAVIFAPTLF--------GDLDLTLEN 311
Cdd:cd04374 115 NAAKSENLESRVNAIHSLVHKLPEKNREMLELLIKHLTNVSDhSKKNLMTVSNLGVVFGPTLLrpqeetvaAIMDIKFQN 194

                ..
gi 17136438 312 VV 313
Cdd:cd04374 195 IV 196

RhoGAP_SYD1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in SYD-1_like proteins. Syd-1, first identified and best studied in C.elegans, has been shown to play an important role in neuronal development by specifying axonal properties. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 160 VPALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRGKSTPHLGNK---DTHTLCCCVKDFLRQLVHPLI-PIYH 235
Cdd:cd04379  18 VPIVLQKCVQEIERRGLDVIGLYRLCGSAAKKKELRDAFERNSAAVELSEElypDINVITGVLKDYLRELPEPLItPQLY 97

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 236 RRDFEEATRH-EDRLAVEMAVYLAVLE-LHQAHRDTLAYLMLHWQKIA-ESPAVRMTVNNLAVIFAPTLF 302
Cdd:cd04379  98 EMVLEALAVAlPNDVQTNTHLTLSIIDcLPLSAKATLLLLLDHLSLVLsNSERNKMTPQNLAVCFGPVLM 167

RhoGAP_PARG1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of PARG1 (PTPL1-associated RhoGAP1). PARG1 was originally cloned as an interaction partner of PTPL1, an intracellular protein-tyrosine phosphatase. PARG1 interacts with Rap2, also a member of the Ras small GTPase superfamily whose exact function is unknown, and shows strong preference for Rho. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 150 SDYAPRVAPMVPALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRGKSTPHLGNKDTHTLCCCVKDFLRQLVHP 229
Cdd:cd04409   6 AQVAKKSPDGIPFIIKKCTSEIESRALCLKGIYRVNGAKSRVEKLCQAFENGKDLVELSELSPHDISNVLKLYLRQLPEP 85

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 230 LIPIYHRRDF-------------EEATRHEDR----LAVEM-AVYLAVLELHQ----AHRDTLAYLMLHWQKIAE-SPAV 286
Cdd:cd04409  86 LILFRLYNEFiglakesqhvnetQEAKKNSDKkwpnMCTELnRILLKSKDLLRqlpaPNYNTLQFLIVHLHRVSEqAEEN 165

                       170
                ....*....|....*
gi 17136438 287 RMTVNNLAVIFAPTL 301
Cdd:cd04409 166 KMSASNLGIIFGPTL 180

RhoGAP_ARHGAP18: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP18-like proteins. The function of ArhGAP18 is unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 159 MVPALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLlrgKSTPHLGNKDTHTLCC-----CVKDFLRQLVHPLIPI 233
Cdd:cd04391  21 KVPLIFQKLINKLEERGLETEGILRIPGSAQRVKFLCQEL---EAKFYEGTFLWDQVKQhdaasLLKLFIRELPQPLLTV 97

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 17136438 234 YHRRDF---EEATRHEDRLaveMAVYLAVLELHQAHRDTLAYLMLHWQKIAESPAV-RMTVNNLAVIFAPTLF 302
Cdd:cd04391  98 EYLPAFysvQGLPSKKDQL---QALNLLVLLLPEANRDTLKALLEFLQKVVDHEEKnKMNLWNVAMIMAPNLF 167

RhoGAP_CdGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of CdGAP-like proteins; CdGAP contains an N-terminal RhoGAP domain and a C-terminal proline-rich region, and it is active on both Cdc42 and Rac1 but not RhoA. CdGAP is recruited to focal adhesions via the interaction with the scaffold protein actopaxin (alpha-parvin). Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 146 RGC-LSDYAPRVAPMVPALIVHCVTEIEARGLQqEGLYRVSSTREKCKRLRRKLLRGKsTPHLGN----KDTHTLCCCVK 220
Cdd:cd04384   3 FGCdLTEHLLNSGQDVPQVLKSCTEFIEKHGIV-DGIYRLSGIASNIQRLRHEFDSEQ-IPDLTKdvyiQDIHSVSSLCK 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 221 DFLRQLVHPLIPIYHRRDFEEATRH---EDRLAvemAVYLAVLELHQAHRDTLAYLMLHWQKIAESPAV-RMTVNNLAVI 296
Cdd:cd04384  81 LYFRELPNPLLTYQLYEKFSEAVSAasdEERLE---KIHDVIQQLPPPHYRTLEFLMRHLSRLAKYCSItNMHAKNLAIV 157

                ....*.
gi 17136438 297 FAPTLF 302
Cdd:cd04384 158 WAPNLL 163

RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been identified as a GAP for Rac1 and Cdc42. Short isoforms (without the PH domain) of ARHGAP24, called RC-GAP72 and p73RhoGAP, and of ARHGAP22, called p68RacGAP, has been shown to be involved in angiogenesis and endothelial cell capillary formation. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 148 CLSD-------YAPRVAPMvpaLIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRGKSTPHLGNKDTHTLCCCVK 220
Cdd:cd04390   6 RLEDtvayerkFGPRLVPI---LVEQCVDFIREHGLKEEGLFRLPGQANLVKQLQDAFDAGERPSFDSDTDVHTVASLLK 82

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 221 DFLRQLVHPLIPIYHRRDF---------EEATRHEDRLAvemavylAVLELHQAHRDTLAYLMLHWQKIAESPAV-RMTV 290
Cdd:cd04390  83 LYLRELPEPVIPWAQYEDFlscaqllskDEEKGLGELMK-------QVSILPKVNYNLLSYICRFLDEVQSNSSVnKMSV 155

                       170
                ....*....|..
gi 17136438 291 NNLAVIFAPTLF 302
Cdd:cd04390 156 QNLATVFGPNIL 167

RhoGAP_ARHGAP20: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP20-like proteins. ArhGAP20, also known as KIAA1391 and RA-RhoGAP, contains a RhoGAP, a RA, and a PH domain, and ANXL repeats. ArhGAP20 is activated by Rap1 and induces inactivation of Rho, which in turn leads to neurite outgrowth. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 148 CLSDYAPRVAPmvPALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRGKStPHLGNKDTHTLCCCVKDFLRQLV 227
Cdd:cd04402   5 PLSNICEDDNL--PKPILDMLSLLYQKGPSTEGIFRRSANAKACKELKEKLNSGVE-VDLKAEPVLLLASVLKDFLRNIP 81

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 17136438 228 HPLIPIYHRRDFEEATRHEDRLAVEMAVYLAVLELHQAHRDTLAYLMLHWQKIAESPAV-RMTVNNLAVIFAPTL 301
Cdd:cd04402  82 GSLLSSDLYEEWMSALDQENEEEKIAELQRLLDKLPRPNVLLLKHLICVLHNISQNSETnKMDAFNLAVCIAPSL 156

RhoGAP_p85: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in the p85 isoforms of the regulatory subunit of the class IA PI3K (phosphatidylinositol 3'-kinase). This domain is also called Bcr (breakpoint cluster region protein) homology (BH) domain. Class IA PI3Ks are heterodimers, containing a regulatory subunit (p85) and a catalytic subunit (p110) and are activated by growth factor receptor tyrosine kinases (RTKs); this activation is mediated by the p85 subunit. p85 isoforms, alpha and beta, contain a C-terminal p110-binding domain flanked by two SH2 domains, an N-terminal SH3 domain, and a RhoGAP domain flanked by two proline-rich regions. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 149 LSDYAPRVAP--MVPALIVHCVTEIEARGLQQEGLYRVSSTREKCKrLRRKLLRGKSTPHLGNKDTHTLCCCVKDFLRQL 226
Cdd:cd04388   2 LPDLTEQFSPpdVAPPLLIKLVEAIEKKGLESSTLYRTQSSSSLTE-LRQILDCDAASVDLEQFDVAALADALKRYLLDL 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 227 VHPLIP--IYHrrdfEEATRHEDRLAVEMAVYL-------AVLELHQAHrdTLAYLMLHWQKIAE-SPAVRMTVNNLAVI 296
Cdd:cd04388  81 PNPVIPapVYS----EMISRAQEVQSSDEYAQLlrklirsPNLPHQYWL--TLQYLLKHFFRLCQsSSKNLLSARALAEI 154

                ....*.
gi 17136438 297 FAPTLF 302
Cdd:cd04388 155 FSPLLF 160

RhoGAP_fBEM3: RhoGAP (GTPase-activator [GAP] protein for Rho-like small GTPases) domain of fungal BEM3-like proteins. Bem3 is a GAP protein of Cdc42, and is specifically involved in the control of the initial assembly of the septin ring in yeast bud formation. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 160 VPALIVHCVTEIEA-RGLQQEGLYRVSSTREKCKRLRRK--------LLRGKSTPhlgnkDTHTLCCCVKDFLRQLVHPL 230
Cdd:cd04400  22 LPSVVYRCIEYLDKnRAIYEEGIFRLSGSASVIKQLKERfnteydvdLFSSSLYP-----DVHTVAGLLKLYLRELPTLI 96

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 17136438 231 IPIYHRRDFEEATRH-EDR--LAVEMAVYLAvlELHQAHRDTLAYLMLHWQKIAESPAV-RMTVNNLAVIFAPTL 301
Cdd:cd04400  97 LGGELHNDFKRLVEEnHDRsqRALELKDLVS--QLPQANYDLLYVLFSFLRKIIEHSDVnKMNLRNVCIVFSPTL 169

RhoGAP_KIAA1688: GTPase-activator protein (GAP) domain for Rho-like GTPases found in KIAA1688-like proteins; KIAA1688 is a protein of unknown function that contains a RhoGAP domain and a myosin tail homology 4 (MyTH4) domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 160 VPALIVHCVTEIEA-RGLQQEGLYRVSSTREKCKRLRRKLLRGKsTPHLGNKDTHTLCCCVKDFLRQLVHPLIPIYHrrd 238
Cdd:cd04389  21 LPWILTFLSEKVLAlGGFQTEGIFRVPGDIDEVNELKLRVDQWD-YPLSGLEDPHVPASLLKLWLRELEEPLIPDAL--- 96

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 239 FEEATRHED--RLAVEMAVYLAVLelhqaHRDTLAYLMLHWQKIAESPAV---RMTVNNLAVIFAPTLF----GDLDLTL 309
Cdd:cd04389  97 YQQCISASEdpDKAVEIVQKLPII-----NRLVLCYLINFLQVFAQPENVahtKMDVSNLAMVFAPNILrctsDDPRVIF 171

                       170
                ....*....|....
gi 17136438 310 ENVVTWQRVLKVLL 323
Cdd:cd04389 172 ENTRKEMSFLRTLI 185

RhoGAP_OCRL1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in OCRL1-like proteins. OCRL1 (oculocerebrorenal syndrome of Lowe 1)-like proteins contain two conserved domains: a central inositol polyphosphate 5-phosphatase domain and a C-terminal Rho GAP domain, this GAP domain lacks the catalytic residue and therefore maybe inactive. OCRL-like proteins are type II inositol polyphosphate 5-phosphatases that can hydrolyze lipid PI(4,5)P2 and PI(3,4,5)P3 and soluble Ins(1,4,5)P3 and Ins(1,3,4,5)P4, but their individual specificities vary. The functionality of the RhoGAP domain is still unclear. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 142 IGTKRGCLSDYAPRVAPMVPALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRG--KSTPHLGNKDTHTLCCCV 219
Cdd:cd04380  32 IDQLELGDNPDYSEVPLSIPKEIWRLVDYLYTRGLAQEGLFEEPGLPSEPGELLAEIRDAldTGSPFNSPGSAESVAEAL 111

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 220 KDFLRQLVHPLIPIYHRRDFEEATRHEDRLAvemavyLAVLELHQA--HRDTLAYLMLHWQKIAESPAVR-MTVNNLAVI 296
Cdd:cd04380 112 LLFLESLPDPIIPYSLYERLLEAVANNEEDK------RQVIRISLPpvHRNVFVYLCSFLRELLSESADRgLDENTLATI 185

                ....*.
gi 17136438 297 FAPTLF 302
Cdd:cd04380 186 FGRVLL 191

RhoGAP_srGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in srGAPs. srGAPs are components of the intracellular part of Slit-Robo signalling pathway that is important for axon guidance and cell migration. srGAPs contain an N-terminal FCH domain, a central RhoGAP domain and a C-terminal SH3 domain; this SH3 domain interacts with the intracellular proline-rich-tail of the Roundabout receptor (Robo). This interaction with Robo then activates the rhoGAP domain which in turn inhibits Cdc42 activity. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136438 146 RGCLSDYAPRVAPMVPALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRGKS--TPHLGNKDTHTLCCCVKDFL 223
Cdd:cd04383   4 NGSLEEYIQDSGQAIPLVVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNAFERGEDplADDQNDHDINSVAGVLKLYF 83

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 17136438 224 RQLVHPLIPIYHRRDFEEATRHEDRLAVEMAVYLAVLELHQAHRDTLAYLMLHWQKIAE-SPAVRMTVNNLAVIFAPTL 301
Cdd:cd04383  84 RGLENPLFPKERFEDLMSCVKLENPTERVHQIREILSTLPRSVIIVMRYLFAFLNHLSQfSDENMMDPYNLAICFGPTL 162