calponin homology (CH) domain found in Drosophila melanogaster muscle-specific protein 20 (dMP20) and similar domains; This subfamily contains Drosophila melanogaster muscle-specific protein 20 (dMP20), Echinococcus granulosus myophilin, Dictyostelium discoideum Rac guanine nucleotide exchange factor B (also called Trix), and similar proteins. dMP20 is present only in the synchronous muscles of D. melanogaster. It may be involved in the system linking the nerve impulse with the contraction or the relaxation process. Trix is involved in the regulation of the late steps of the endocytic pathway. dMP20 contains a single copy of the CH domain, while Trix (triple CH-domain array exchange factor) contains three, two type 3 CH domains which are included in this model, and one type 1 CH domain that is not included in this subfamily, but is part of the superfamily. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  14 KRNPEMDKEAQEWIEAIIAEKFPAGQSYEDVLKDGQVLCKLINVLSPNAVPKVNSSGGQFKFMENINNFQKALKEYGVPD 93
Cdd:cd21207   1 KRDPALEAEALDWIEAVTGEKLDDGKDYEDVLKDGVILCKLINILKPGSVKKINTSKMAFKLMENIENFLTACKGYGVPK 80

                        90       100
                ....*....|....*....|....*..
gi 17136346  94 IDVFQTVDLYEKKDIANVTNTIFALGR 120
Cdd:cd21207  81 TDLFQTVDLYEKKNIPQVTNCLFALGR 107

calponin homology (CH) domain found in Drosophila melanogaster muscle-specific protein 20 (dMP20) and similar domains; This subfamily contains Drosophila melanogaster muscle-specific protein 20 (dMP20), Echinococcus granulosus myophilin, Dictyostelium discoideum Rac guanine nucleotide exchange factor B (also called Trix), and similar proteins. dMP20 is present only in the synchronous muscles of D. melanogaster. It may be involved in the system linking the nerve impulse with the contraction or the relaxation process. Trix is involved in the regulation of the late steps of the endocytic pathway. dMP20 contains a single copy of the CH domain, while Trix (triple CH-domain array exchange factor) contains three, two type 3 CH domains which are included in this model, and one type 1 CH domain that is not included in this subfamily, but is part of the superfamily. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  14 KRNPEMDKEAQEWIEAIIAEKFPAGQSYEDVLKDGQVLCKLINVLSPNAVPKVNSSGGQFKFMENINNFQKALKEYGVPD 93
Cdd:cd21207   1 KRDPALEAEALDWIEAVTGEKLDDGKDYEDVLKDGVILCKLINILKPGSVKKINTSKMAFKLMENIENFLTACKGYGVPK 80

                        90       100
                ....*....|....*....|....*..
gi 17136346  94 IDVFQTVDLYEKKDIANVTNTIFALGR 120
Cdd:cd21207  81 TDLFQTVDLYEKKNIPQVTNCLFALGR 107

Calponin homology (CH) domain; The CH domain is found in both cytoskeletal proteins and signal transduction proteins. The CH domain is involved in actin binding in some members of the family. However in calponins there is evidence that the CH domain is not involved in its actin binding activity. Most member proteins have from two to four copies of the CH domain, however some proteins such as calponin have only a single copy.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346    17 PEMDKEAQEWIEAIIAEKFP--AGQSYEDVLKDGQVLCKLINVLSPNAVPKVNSSGGQFKFMENINNF-QKALKEYGVPD 93
Cdd:pfam00307   1 LELEKELLRWINSHLAEYGPgvRVTNFTTDLRDGLALCALLNKLAPGLVDKKKLNKSEFDKLENINLAlDVAEKKLGVPK 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 17136346    94 IDVfQTVDLYEKKDIANVTnTIFALGRATYK 124
Cdd:pfam00307  81 VLI-EPEDLVEGDNKSVLT-YLASLFRRFQA 109

Calponin family repeat;

                          10        20
                  ....*....|....*....|....*
gi 17136346   157 VGLQAGSNKGATQAGQN-LGAGRKI 180
Cdd:pfam00402   1 IGLQMGTNKGASQSGMTaFGAVRHI 25

calponin homology (CH) domain found in Drosophila melanogaster muscle-specific protein 20 (dMP20) and similar domains; This subfamily contains Drosophila melanogaster muscle-specific protein 20 (dMP20), Echinococcus granulosus myophilin, Dictyostelium discoideum Rac guanine nucleotide exchange factor B (also called Trix), and similar proteins. dMP20 is present only in the synchronous muscles of D. melanogaster. It may be involved in the system linking the nerve impulse with the contraction or the relaxation process. Trix is involved in the regulation of the late steps of the endocytic pathway. dMP20 contains a single copy of the CH domain, while Trix (triple CH-domain array exchange factor) contains three, two type 3 CH domains which are included in this model, and one type 1 CH domain that is not included in this subfamily, but is part of the superfamily. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  14 KRNPEMDKEAQEWIEAIIAEKFPAGQSYEDVLKDGQVLCKLINVLSPNAVPKVNSSGGQFKFMENINNFQKALKEYGVPD 93
Cdd:cd21207   1 KRDPALEAEALDWIEAVTGEKLDDGKDYEDVLKDGVILCKLINILKPGSVKKINTSKMAFKLMENIENFLTACKGYGVPK 80

                        90       100
                ....*....|....*....|....*..
gi 17136346  94 IDVFQTVDLYEKKDIANVTNTIFALGR 120
Cdd:cd21207  81 TDLFQTVDLYEKKNIPQVTNCLFALGR 107

calponin homology (CH) domain found in Saccharomyces cerevisiae transgelin (SCP1) and similar proteins; The family includes transgelins from Saccharomyces cerevisiae and Schizosaccharomyces pombe, which are also called SCP1 and STG1, respectively. Transgelin, also called calponin homolog 1, has actin-binding and actin-bundling activity. It stabilizes actin filaments against disassembly. Transgelin contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  19 MDKEAQEWIEAIIAEKFPAGqSYEDVLKDGQVLCKLINVLSPNAVPKVNSSGGQFKFMENINNFQKALKEYGVPDIDVFQ 98
Cdd:cd21210   1 AEQEAREWIEEVLGEKLAQG-DLLDALKDGVVLCKLANRILPADIRKYKESKMPFVQMENISAFLNAARKLGVPENDLFQ 79

                        90       100
                ....*....|....*....|..
gi 17136346  99 TVDLYEKKDIANVTNTIFALGR 120
Cdd:cd21210  80 TVDLFERKNPAQVLQCLHALSR 101

calponin homology (CH) domain found in transgelin-3; Transgelin-3, also called neuronal protein 22 (NP22), or neuronal protein NP25, may have a role in alcohol-related adaptations and may mediate regulatory signal transduction pathways in neurons. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  16 NPEMDKEAQEWIEAIIAEKF----PAGQSYEDVLKDGQVLCKLINVLSPNA---VPKVNSSGGQFKFMENINNFQKALKE 88
Cdd:cd21281   1 DPDLESRLVDWIIIQCGGDIerpqPGRQNFQKWLMDGTILCRLINSLYPPGkepIKKISETKMAFKQMEKISQFLQAAEA 80

                        90       100       110
                ....*....|....*....|....*....|.
gi 17136346  89 YGVPDIDVFQTVDLYEKKDIANVTNTIFALG 119
Cdd:cd21281  81 YGVITTDIFQTVDLWEGKDMAAVQRTLMALG 111

calponin homology (CH) domain found in the IQ motif containing GTPase activating protein family; Members of the IQ motif containing GTPase activating protein (IQGAP) family are associated with the Ras GTP-binding protein and act as essential regulators of cytoskeletal function. There are three known IQGAP family members: IQGAP1, IQGAP2, and IQGAP3. They are multi-domain molecules having a calponin-homology (CH) domain which binds F-actin, IQGAP-specific repeats, a single WW domain, four IQ motifs that mediate interactions with calmodulin, and a RasGAP related domain that binds active Rho family GTPases. IQGAP1 negatively regulates Ras family GTPases by stimulating their intrinsic GTPase activity. It lacks GAP activity. Both IQGAP1 and IQGAP2 specifically bind to Cdc42 and Rac1, but not to RhoA. Despite similarities to part of the sequence of RasGAP, neither IQGAP1 nor IQGAP2 interacts with Ras. IQGAP3 regulates the organization of the cytoskeleton under the regulation of Rac1 and Cdc42 in neuronal cells. The depletion of IQGAP3 is shown to impair neurite or axon outgrowth in neuronal cells with disorganized cytoskeleton.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  22 EAQEWIEAIIAEKFPAGQSYEDVLKDGQVLCKLINVLSPNAVP--KVNSSGGQFKFMENINNFQKALKEYGVPDIDVFQT 99
Cdd:cd21206  12 EAKQWIEACLNEELPPTTEFEEELRNGVVLAKLANKFAPKLVPlkKIYDVGLQFRHTDNINHFLRALKKIGLPKIFHFET 91

                        90       100
                ....*....|....*....|....*.
gi 17136346 100 VDLYEKKDIANVTNTIFALGRATYKH 125
Cdd:cd21206  92 TDLYEKKNIPKVIYCLHALSLLLFKL 117

Calponin homology (CH) domain; The CH domain is found in both cytoskeletal proteins and signal transduction proteins. The CH domain is involved in actin binding in some members of the family. However in calponins there is evidence that the CH domain is not involved in its actin binding activity. Most member proteins have from two to four copies of the CH domain, however some proteins such as calponin have only a single copy.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346    17 PEMDKEAQEWIEAIIAEKFP--AGQSYEDVLKDGQVLCKLINVLSPNAVPKVNSSGGQFKFMENINNF-QKALKEYGVPD 93
Cdd:pfam00307   1 LELEKELLRWINSHLAEYGPgvRVTNFTTDLRDGLALCALLNKLAPGLVDKKKLNKSEFDKLENINLAlDVAEKKLGVPK 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 17136346    94 IDVfQTVDLYEKKDIANVTnTIFALGRATYK 124
Cdd:pfam00307  81 VLI-EPEDLVEGDNKSVLT-YLASLFRRFQA 109

calponin homology (CH) domain found in calponin-1 and similar proteins; Calponin-1 (CNN1), also called basic calponin, or smooth muscle calponin H1, is a thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C, and tropomyosin. Calponin-1 contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  16 NPEMDKEAQEWIEAIIAEKFpaGQSYEDVLKDGQVLCKLINVLSPNAVPKVNSSGGQFKFMENINNFQKALKEYGVPDID 95
Cdd:cd21282   1 DPQTEEELRVWIEGVTGRRI--GDNFMDGLKDGVILCELINKLQPGSVRKINESTQNWHKLENIGNFIKAIMHYGVKPHD 78

                        90       100
                ....*....|....*....|...
gi 17136346  96 VFQTVDLYEKKDIANVTNTIFAL 118
Cdd:cd21282  79 IFEANDLFENTNHTQVQSTLIAL 101

Calponin homology domain; Actin binding domains present in duplicate at the N-termini of spectrin-like proteins (including dystrophin, alpha-actinin). These domains cross-link actin filaments into bundles and networks. A calponin homology domain is predicted in yeasst Cdc24p.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346     21 KEAQEWIEAIIAE-KFPAGQSYEDVLKDGQVLCKLINVLSPNAVPK--VNSSGGQFKFMENINNFQKALKEYGvPDIDVF 97
Cdd:smart00033   1 KTLLRWVNSLLAEyDKPPVTNFSSDLKDGVALCALLNSLSPGLVDKkkVAASLSRFKKIENINLALSFAEKLG-GKVVLF 79

                           90       100
                   ....*....|....*....|..
gi 17136346     98 QTVDLYE-KKDIANVTNTIFAL 118
Cdd:smart00033  80 EPEDLVEgPKLILGVIWTLISL 101

calponin homology (CH) domain found in calponin-3; Calponin-3 (CNN3), also called acidic isoform calponin, is an F-actin-binding protein that is expressed in the brain and has been shown to control dendritic spine morphology, density, and plasticity by regulating actin cytoskeletal reorganization and dynamics. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  14 KRNPEMDKEAQEWIEAIIAekFPAGQSYEDVLKDGQVLCKLINVLSPNAVPKVNSSGGQFKFMENINNFQKALKEYGVPD 93
Cdd:cd21284   1 KYDPQKEEELRNWIEEVTG--MSIGENFQKGLKDGVILCELINKLQPGSIRKINESKLNWHQLENIGNFIKAIQAYGMKP 78

                        90       100
                ....*....|....*....|....*
gi 17136346  94 IDVFQTVDLYEKKDIANVTNTIFAL 118
Cdd:cd21284  79 HDIFEANDLFENGNMTQVQTTLLAL 103

calponin homology (CH) domain found in transgelin; Transgelin, also called 22 kDa actin-binding protein, protein WS3-10, or smooth muscle protein 22-alpha (SM22-alpha), acts as an actin cross-linking/gelling protein that may be involved in calcium interactions and in regulating contractile properties of the cell. It may also contribute to replicative senescence. Transgelin contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 17136346  45 LKDGQVLCKLINVLSPNAVPKV----NSSGGQFKFMENINNFQKALKEYGVPDIDVFQTVDLYEKKDIANVTNTIFALG 119
Cdd:cd21279  34 LKNGVVLSKLVNSLYPDGSKPVkipdNPPSMVFKQMEQVAQFLKAAEDYGVVKTDMFQTVDLYEGKDMAAVQRTLVALG 112

calponin homology (CH) domain found in Arabidopsis thaliana Kinesin-like KIN-14 protein family; Kinesins are microtubule-dependent molecular motors that play important roles in intracellular transport and in cell division. This family includes a group of kinesin-like proteins belonging to KIN-14 protein family. They all contain a single copy of the CH domain at the N-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  21 KEAQEWIEAIIAEKFPAGQSYEDV---LKDGQVLCKLINVLSPNAVPKVNSS--------GGQFKFMENINNFQKALKEY 89
Cdd:cd21203   3 YEAAEWIQNVLGVLVLPDPSEEEFrlcLRDGVVLCKLLNKLQPGAVPKVVESpddpdgaaGSAFQYFENVRNFLVAIEEM 82

                        90       100       110
                ....*....|....*....|....*....|.
gi 17136346  90 GVPdidVFQTVDLYEKK--DIANVTNTIFAL 118
Cdd:cd21203  83 GLP---TFEASDLEQGGggSRPRVVDCILAL 110

calponin homology (CH) domain found in Ras GTPase-activating-like protein IQGAP1; IQ motif containing GTPase activating protein 1 (IQGAP1), also called p195, is a homodimeric protein that is widely expressed among vertebrate cell types from early embryogenesis. It plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. It belongs to the IQGAP family, which consists of multi-domain proteins having a calponin-homology (CH) domain which binds F-actin, IQGAP-specific repeats, a single WW domain, four IQ motifs that mediate interactions with calmodulin, and a RasGAP related domain that binds active Rho family GTPases. IQGAP1 negatively regulates Ras family GTPases by stimulating their intrinsic GTPase activity. It lacks GAP activity. Both, IQGAP1 and IQGAP2, specifically bind to Cdc42 and Rac1, but not to RhoA. Despite similarities to part of the sequence of RasGAP, neither IQGAP1 nor IQGAP2 interacts with Ras. IQGAP1 contains a single copy of the CH domain at the N-terminus.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  21 KEAQEWIEAIIAEKFPAGQSYEDVLKDGQVLCKLINVLSPNAVP----------KVNSSGGQFKFMENINNFQKALKEYG 90
Cdd:cd21274  12 EEAKRWMEACLGEDLPPTTELEEGLRNGVYLAKLGNFFSPKVVSlkkiydreqtRYKATGLHFRHTDNVIQWLNAMDEIG 91

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 17136346  91 VPDIDVFQTVDLYEKKDIANVTNTIFALGRATYKHAdfkgpfLGPKPAD-ECKRDFTEEQL 150
Cdd:cd21274  92 LPKIFYPETTDIYDRKNMPRCIYCIHALSLYLFKLG------LAPQIQDlYGKVDFTEEEI 146

calponin homology (CH) domain found in the Pak Interactive eXchange factor family; Pak Interactive eXchange factor (PIX) proteins are Rho guanine nucleotide exchange factors (GEFs), which activate small GTPases by exchanging bound GDP for free GTP. They act as GEFs for both Cdc42 and Rac1, and have been implicated in cell motility, adhesion, neurite outgrowth, and cell polarity. Vertebrates contain two proteins from the PIX family, alpha-PIX and beta-PIX. Alpha-PIX, also called Rho guanine nucleotide exchange factor 6 (ARHGEF6), is localized in dendritic spines where it regulates spine morphogenesis. It controls dendritic length and spine density in the hippocampus. Mutations in the ARHGEF6 gene cause X-linked intellectual disability in humans. Beta-PIX, also called Rho guanine nucleotide exchange factor 7 (ARHGEF7), plays important roles in regulating neuroendocrine exocytosis, focal adhesion maturation, cell migration, synaptic vesicle localization, and insulin secretion. Both alpha-PIX and beta-PIX contain a single copy of the CH domain at the N-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  43 DVLKDGQVLCKLINVLSPNAVPKV-NSSGGQFKFMENINNFQKALKEYGVPDIDVFQTVDLYEKKDIANVTNTIFALGRA 121
Cdd:cd21202  32 ESLKNGVVLCRLVNRLKPGTVEKIyDEPTTEEECLYNFESFLKACQELGILAEEIFDPNDLYSGGNFQKVLSTLERLEKV 111

                .
gi 17136346 122 T 122
Cdd:cd21202 112 A 112

calponin homology (CH) domain found in LIM domain only protein 7; LIM domain only protein 7 (LMO-7), also called F-box only protein 20, or LOMP, is a transcription regulator for expression of many Emery-Dreifuss muscular dystrophy (EDMD)-relevant genes. It binds to alpha-actinin and AF6/afadin at adherens junctions for epithelial cell-cell adhesion. It contains a single copy of the CH domain at the N-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  22 EAQEWIEAIIAEKFpAGQSYEDVLKDGQVLCKLINVLSPNAVPKVNSSGGQFKFMENINNFQKALKEYGVPDIDVFQTVD 101
Cdd:cd21277   4 EAQRWIEAVTGKNF-GNKDFRSALENGVLLCDLINKIKPGIIKKINRLSTPIAGLDNINVFLKACEKLGLKEAQLFHPGD 82

                        90       100       110
                ....*....|....*....|....*....|.
gi 17136346 102 LYE------------KKDIANVTNTIFALGR 120
Cdd:cd21277  83 LQDlstrvtvkqeetDRRLKNVLITLYWLGR 113

calponin homology (CH) domain found in the leucine-rich repeat and calponin homology domain-containing protein family; The leucine-rich repeat and calponin homology domain-containing protein (LRCH) family includes LRCH1-4. LRCH1, also called calponin homology domain-containing protein 1, or neuronal protein 81 (NP81), acts as a negative regulator of GTPase Cdc42 by sequestering Cdc42-guanine exchange factor DOCK8. LRCH2 may play a role in the organization of the cytoskeleton. LRCH3 is part of the DISP complex and may regulate the association of septins with actin and thereby regulate the actin cytoskeleton. LRCH4, also called leucine-rich repeat neuronal protein 4, or leucine-rich neuronal protein, acts as a novel Toll-like receptor (TLR) accessory protein that regulates the innate immune response. Members of this family contain a single copy of the CH domain at the C-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  45 LKDGQVLCKLINVLSPNAVPK--VNSSG----GQFKFMENINNFQKALKEYGVPDIDVFQTVDLYEKKDIANVTNTIFAL 118
Cdd:cd21205  26 LMDGVVLCHLANHVRPRSVPSihVPSPAvpklSMAKCRRNVENFLEACRKLGVPEERLCSPGDILEEKGLVRVAVTVQAL 105

calponin homology (CH) domain found in VAV1 protein; VAV1 is expressed predominantly in the hematopoietic system and it plays an important role in the development and activation of B and T cells. It is activated by tyrosine phosphorylation to function as a guanine nucleotide exchange factor (GEF) for Rho GTPases following cell surface receptor activation, triggering various effects such as cytoskeletal reorganization, transcription regulation, cell cycle progression, and calcium mobilization. It also serves as a scaffold protein and has been shown to interact with Ku70, Socs1, Janus kinase 2, SIAH2, S100B, Abl gene, ZAP-70, SLP76, and Syk, among others. VAV proteins contain several domains that enable their function: N-terminal calponin homology (CH), acidic, RhoGEF (also called Dbl-homologous or DH), Pleckstrin Homology (PH), C1 (zinc finger), SH2, and two SH3 domains. This model corresponds to the CH domain, an actin-binding domain which is present as a single copy in VAV1 protein.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 17136346  45 LKDGQVLCKLINVLSPNAV--PKVN--SSGGQFKFMENINNFQKALKE-YGVPDIDVFQTVDLYEKKDIANVTNTIFAL 118
Cdd:cd21262  36 LRDGVLLCQLLNNLLPHAVnlREINlrPQMSQFLCLKNIRTFLSTCCEkFGLRKSELFEAFDLFDVRDFGKVIDTLSIL 114

calponin homology (CH) domain found in beta-Pak Interactive eXchange factor; Beta-Pak Interactive eXchange factor (beta-PIX), also called PAK-interacting exchange factor beta, Rho guanine nucleotide exchange factor 7 (ARHGEF7), p85, or Cool (Cloned out of Library)-1, activates small GTPases by exchanging bound GDP for free GTP. It acts as a GEF for both Cdc42 and Rac1, and plays important roles in regulating neuroendocrine exocytosis, focal adhesion maturation, cell migration, synaptic vesicle localization, and insulin secretion. Beta-PIX contains a single copy of the CH domain at its N-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 17136346  45 LKDGQVLCKLINVLSPNAVPKV-NSSGGQFKFMENINNFqkaLKEYGVPDIDVFQTVDLYEKKDIANVTNTIFALGRAT 122
Cdd:cd21266  33 LKDGVVLCRLLERLLPGSIDKVyPEPRTESECLSNIREF---LRGCGALRLETFDANDLYQGQNFNKVLSSLVALNKVT 108

calponin homology (CH) domain found in leucine-rich repeat and calponin homology domain-containing protein 3; Leucine-rich repeat and calponin homology domain-containing protein 3 (LRCH3) is part of the DISP (DOCK7-Induced Septin disPlacement) complex. It may regulate the association of septins with actin and thereby regulate the actin cytoskeleton. LRCH3 contains a single copy of the CH domain at the C-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  45 LKDGQVLCKLINVLSPNAVPKVNSSGGQF------KFMENINNFQKALKEYGVPDIDVFQTVDLYEKKDIANVTNTIFAL 118
Cdd:cd21272  26 LTDGVVLCHLANHVRPRSVPSIHVPSPAVpkltmaKCRRNVENFLEACRRIGVPQEQLCLPLHILEEKGLSQVAVTVQAL 105

first calponin homology (CH) domain found in the plastin/fimbrin family; This family includes plastin and fimbrin. Plastin has three isoforms, plastin-1, -2, and -3, which are all actin-bundling proteins. Plastin-1, also called intestine-specific plastin, or I-plastin, is an actin-bundling protein in the absence of calcium. Plastin-2, also called L-plastin, LC64P, or lymphocyte cytosolic protein 1 (LCP-1), plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. It modulates the cell surface expression of IL2RA/CD25 and CD69. Plastin-3, also called T-plastin, is found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It may play a role in the regulation of bone development. Fimbrin has been found in plants and fungi. Arabidopsis thaliana fimbrin (AtFIM) includes fimbrin-1, -2, -3, -4, and -5; they cross-link actin filaments (F-actin) in a calcium independent manner. They stabilize and prevent F-actin depolymerization mediated by profilin. They act as key regulators of actin cytoarchitecture, probably involved in cell cycle, cell division, cell elongation and cytoplasmic tractus. AtFIM5 is an actin bundling factor that is required for pollen germination and pollen tube growth. Fungal fimbrin binds to actin, and functionally associates with actin structures involved in the development and maintenance of cell polarity. Members of this family contain four copies of the CH domain. This model corresponds to the first CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  25 EWIEAIIAE---------KFPAGQSYEDVLKDGQVLCKLINVLSPNAVP--KVNSSG--GQFKFMENINNFQKALKEYGV 91
Cdd:cd21217   8 EHINSLLADdpdlkhllpIDPDGDDLFEALRDGVLLCKLINKIVPGTIDerKLNKKKpkNIFEATENLNLALNAAKKIGC 87

calponin homology (CH) domain found in leucine-rich repeat and calponin homology domain-containing protein 2; Leucine-rich repeat and calponin homology domain-containing protein 2 (LRCH2) may play a role in the organization of the cytoskeleton. It contains a single copy of the CH domain at the C-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  18 EMDKEAQEWIEAIIAEKFPagqsyEDV---LKDGQVLCKLINVLSPN----------AVPKVNSSggqfKFMENINNFQK 84
Cdd:cd21271   5 ELIEQLRENIESRLKVILP-----EDLgaaLMDGVVLCHLANHIRPRsvgsihvpspAVPKLSMA----KCRRNVENFLD 75

                        90       100       110
                ....*....|....*....|....*....|....
gi 17136346  85 ALKEYGVPDIDVFQTVDLYEKKDIANVTNTIFAL 118
Cdd:cd21271  76 ACRKLGVPEDKLCLPHHILEEKGLVKVSVTVQAL 109

first calponin homology (CH) domain found in spectrin beta chain, non-erythrocytic 4 (SPTBN4) and similar proteins; Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. SPTBN4, also called beta-IV spectrin, or spectrin, non-erythroid beta chain 3 (SPTBN3), is a novel spectrin isolated as an interactor of the receptor tyrosine phosphatase-like protein ICA512. Its mutation associates with congenital myopathy, neuropathy, and central deafness. SPTBN4 contains two copies of the CH domain. This model corresponds to the first CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17136346  11 IASKRNPEMDKEAQEWIEAIIAeKFPA--GQSYEDvLKDGQVLCKLINVLSPNAVPKVNSSGGQFKFMENINNFQKALKE 88
Cdd:cd21318  31 LADEREAVQKKTFTKWVNSHLA-RVPCriNDLYTD-LRDGYVLTRLLEVLSGEQLPKPTRGRMRIHSLENVDKALQFLKE 108

                ...
gi 17136346  89 YGV 91
Cdd:cd21318 109 QRV 111