|
Name |
Accession |
Description |
Interval |
E-value |
| PRK02406 |
PRK02406 |
DNA polymerase IV; Validated |
9-351 |
0e+00 |
|
DNA polymerase IV; Validated
Pssm-ID: 235035 [Multi-domain] Cd Length: 343 Bit Score: 685.31 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 9 MDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIRE 88
Cdd:PRK02406 1 MDCFYAAVEMRDNPELRGKPVAVGGSPGRRGVISTCNYEARKFGVRSAMPTAQALKLCPDLIFVPGRFDVYKEVSRQIRE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 89 IFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQFVITPAE 168
Cdd:PRK02406 81 IFRRYTDLIEPLSLDEAYLDVTDNKLCIGSATLIAQEIRQDIFEELGLTASAGVAPNKFLAKIASDWNKPNGLFVITPEE 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 169 VPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFGRILWERSQGIDERDVNSERLRKSVGVERT 248
Cdd:PRK02406 161 VDAFLATLPVEKIPGVGKVTAEKLHALGIYTCADLQKYDLAELIRHFGKFGRRLYERARGIDERPVKPDRERKSVGVERT 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 249 MAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRG 328
Cdd:PRK02406 241 FAEDLYDLEACLAELPRLAEKLERRLERAKPDKRIKTVGVKLKFADFQQTTKEHTADPLDKADLIELLAQALLRRLGGRG 320
|
330 340
....*....|....*....|...
gi 16128217 329 VRLVGLHVTLLDPQMERQLVLGL 351
Cdd:PRK02406 321 VRLLGVGVTLLEPQLERQLLLDL 343
|
|
| PolY_Pol_IV_kappa |
cd03586 |
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ... |
5-339 |
9.79e-179 |
|
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.
Pssm-ID: 176459 [Multi-domain] Cd Length: 334 Bit Score: 498.58 E-value: 9.79e-179
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 5 IHVDMDCFFAAVEMRDNPALRDIPIAIGGsRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASN 84
Cdd:cd03586 1 IHIDMDAFYASVEQRDNPELKGKPVAVGG-SSDRGVVSTASYEARKFGVRSAMPIFQAKKLCPNLIFVPPRFDKYREVSR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 85 HIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQFVI 164
Cdd:cd03586 80 QIMEILREYTPLVEPLSIDEAYLDVTDYVRLFGSATEIAKEIRARIREETGLTASAGIAPNKFLAKIASDLNKPNGLTVI 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 165 TPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFGRILWERSQGIDERDVNSERLRKSVG 244
Cdd:cd03586 160 PPEDVEEFLAPLPVRKIPGVGKVTAEKLKELGIKTIGDLAKLDVELLKKLFGKSGRRLYELARGIDNRPVEPDRERKSIG 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 245 VERTMAEDIHHWSECEAIIERLYPELERRLAKVKpdLLIARQGVKLKFDDFQQTTQE--HVWPRLNKADLIATARKTWDE 322
Cdd:cd03586 240 VERTFSEDLTDPEELLEELLELAEELAERLRKRG--LKGRTVTVKLKYADFSTRTRSrtLPEPTDDAEDIYELALELLEE 317
|
330
....*....|....*..
gi 16128217 323 RRGGRGVRLVGLHVTLL 339
Cdd:cd03586 318 LLDGRPIRLLGVRLSGL 334
|
|
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
2-337 |
9.34e-156 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 440.35 E-value: 9.34e-156
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 2 RKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGsRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKE 81
Cdd:COG0389 1 RRILHVDMDAFYASVEQRDRPELRGKPVAVGG-DNNRGVVAAASYEARAFGVRSGMPLFQARRLCPDLVVLPPDFELYRD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 82 ASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQ 161
Cdd:COG0389 80 VSRRVMAILERYTPLVEPLSIDEAFLDVTGSARLFGSAEAIARRIRRRIRRETGLTVSVGIAPNKFLAKIASDLAKPDGL 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 162 FVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFGRILWERSQGIDERDVNSERLRK 241
Cdd:COG0389 160 TVIPPGEVAAFLAPLPVEKLWGVGPKTAEKLARLGIRTIGDLAALPRAELRRRFGKVGERLYRLARGIDPRPVEPRRPRK 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 242 SVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVkpDLLIARQGVKLKFDDFQQTTQEHVWPR--LNKADLIATARKT 319
Cdd:COG0389 240 SIGVERTFGEDLTDLEELEAALRRLAERLAERLRRQ--GLGARTVTVKLRTSDFRTTTRSRTLPEptDDTAELLRAAREL 317
|
330
....*....|....*....
gi 16128217 320 WDER-RGGRGVRLVGLHVT 337
Cdd:COG0389 318 LERIyRPGRPVRLLGVRLS 336
|
|
| PRK14133 |
PRK14133 |
DNA polymerase IV; Provisional |
1-349 |
1.37e-97 |
|
DNA polymerase IV; Provisional
Pssm-ID: 184529 [Multi-domain] Cd Length: 347 Bit Score: 293.16 E-value: 1.37e-97
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 1 MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERrGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYK 80
Cdd:PRK14133 2 DRVIIHVDMDAFFASVEQMDNPKLKGKPVIVGGISER-GVVSTCSYEARKYGVHSAMPVFMAKKRCPHGIFLPVRHERYK 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 81 EASNHIREIFSRYTSRIEPLSLDEAYLDVTDsvhCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG 160
Cdd:PRK14133 81 EVSKNIFKILYEVTPIVEPVSIDEAYLDITN---IKEEPIKIAKYIKKKVKKETGLTLSVGISYNKFLAKLASDWNKPDG 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 161 QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFGRILWERSQGIDERDVNSERLR 240
Cdd:PRK14133 158 IKIITEDMIPDILKPLPISKVHGIGKKSVEKLNNIGIYTIEDLLKLSREFLIEYFGKFGVEIYERIRGIDYREVEVSRER 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 241 KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKpdlLIARQ-GVKLKFDDFQQTTQEHVwprLNK-----ADLIA 314
Cdd:PRK14133 238 KSIGKETTLKKDTKDKEELKKYLKDFSNIISEELKKRN---LYGKTvTVKIKTSDFQTHTKSKT---LNDyirdkEEIYN 311
|
330 340 350
....*....|....*....|....*....|....*
gi 16128217 315 TARKTWDERRGGRGVRLVGLHVTLLDPQMERQLVL 349
Cdd:PRK14133 312 VACEILEHINIKEPIRLIGLSVSNLSENKIEQLSF 346
|
|
| PRK01810 |
PRK01810 |
DNA polymerase IV; Validated |
2-340 |
3.09e-96 |
|
DNA polymerase IV; Validated
Pssm-ID: 179337 [Multi-domain] Cd Length: 407 Bit Score: 291.55 E-value: 3.09e-96
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 2 RKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGS-RERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYK 80
Cdd:PRK01810 5 RVIFHVDMNSFFASVEIAYDPSLQGKPLAVAGNeKERKGIIVTCSYEARAYGIRTTMPLWEAKRLCPQLIVRRPNFDRYR 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 81 EASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHChGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG 160
Cdd:PRK01810 85 EASRQMFQILSEFTPLVQPVSIDEGYLDITDCYAL-GSPLEIAKMIQQRLLTELQLPCSIGIAPNKFLAKMASDMKKPLG 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 161 QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFGRILWERSQGIDERDVNSERL- 239
Cdd:PRK01810 164 ITVLRKRDVPEMLWPLPVGEMHGIGEKTAEKLKDIGIQTIGDLAKADEHILRAKLGINGVRLQRRANGIDDRPVDPEAIy 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 240 -RKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKpdlLIARqGVKL--KFDDFQQTTQEHVW--PRLNKADLIA 314
Cdd:PRK01810 244 qFKSVGNSTTLSHDMDEEKELLDVLRRLSKSVSKRLQKKT---VVSY-NVQImiRYHDRRTITRSKTLknPIWEKRDIFQ 319
|
330 340
....*....|....*....|....*.
gi 16128217 315 TARKTWDERRGGRGVRLVGlhVTLLD 340
Cdd:PRK01810 320 AASRLFKQHWNGDPVRLLG--VTATD 343
|
|
| PRK02794 |
PRK02794 |
DNA polymerase IV; Provisional |
4-345 |
4.08e-96 |
|
DNA polymerase IV; Provisional
Pssm-ID: 179473 [Multi-domain] Cd Length: 419 Bit Score: 291.84 E-value: 4.08e-96
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 4 IIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRerRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEAS 83
Cdd:PRK02794 38 IAHIDCDAFYASVEKRDNPELRDKPVIIGGGK--RGVVSTACYIARIHGVRSAMPMFKALKLCPDAVVIKPDMEKYVRVG 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 84 NHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGS--ATLIAQEIRQtIFNELQLTASAGVAPVKFLAKIASDMNKPNGQ 161
Cdd:PRK02794 116 REVRAMMQALTPLVEPLSIDEAFLDLSGTERLHGAppAVVLARFARR-VEREIGITVSVGLSYNKFLAKIASDLDKPRGF 194
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 162 FVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFGRILWERSQGIDERDVNSERLRK 241
Cdd:PRK02794 195 SVIGRAEALAFLAPKPVGIIWGVGPATAARLARDGIRTIGDLQRADEADLMRRFGSMGLRLWRLARGIDDRKVSPDREAK 274
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 242 SVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKpdllIARQGV--KLKFDDFQQTTQEHvwpRLNK----ADLI-A 314
Cdd:PRK02794 275 SVSAETTFETDLSDFEDLEPILWRLSEKVSRRLKAAG----LAGRTVtlKLKTADFRLRTRRR---TLEDptqlADRIfR 347
|
330 340 350 360
....*....|....*....|....*....|....*....|...
gi 16128217 315 TARKTWDERRGGRGVRLVGLHVT------------LLDPQMER 345
Cdd:PRK02794 348 TARELLEKETDGTAFRLIGIGVSdlspadeadppdLLDPQATR 390
|
|
| PolY_Rev1 |
cd01701 |
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. ... |
2-291 |
2.82e-80 |
|
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Rev1 has both structural and enzymatic roles. Structurally, it is believed to interact with other nonclassical polymerases and replication machinery to act as a scaffold. Enzymatically, it catalyzes the specific insertion of dCMP opposite abasic sites. Rev1 interacts with the Rev7 subunit of the B-family TLS polymerase Pol zeta (Rev3/Rev7). Rev1 is known to actively promote the introduction of mutations, potentially making it a significant target for cancer treatment.
Pssm-ID: 176455 [Multi-domain] Cd Length: 404 Bit Score: 250.69 E-value: 2.82e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 2 RKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKE 81
Cdd:cd01701 47 RIIMHVDFDCFFVSVSIRNRPDLKGKPVAVCHGKGPNSEIASCNYEARSYGIKNGMWVGQAKKLCPQLVTLPYDFEAYEE 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 82 ASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHG-SATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG 160
Cdd:cd01701 127 VSLTFYEILASYTDNIEAVSCDEALIDITSLLEETYeLPEELAEAIRNEIRETTGCSASVGIGPNILLARLATRKAKPDG 206
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 161 QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVM--LLKRFG-KFGRILWERSQGIDERDVNSE 237
Cdd:cd01701 207 QYHLSAEKVEEFLSQLKVGDLPGVGSSLAEKLVKLFGDTCGGLELRSKTKekLQKVLGpKTGEKLYDYCRGIDDRPVTGE 286
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 238 RLRKSVGVE-----RTMAEDihhwsECEAIIERLYPELERRLAKVKpdlLIARQ-GVKLK 291
Cdd:cd01701 287 KERKSVSAEinygiRFTNVD-----DVEQFLQRLSEELSKRLEESN---VTGRQiTLKLM 338
|
|
| PRK03348 |
PRK03348 |
DNA polymerase IV; Provisional |
1-333 |
6.29e-79 |
|
DNA polymerase IV; Provisional
Pssm-ID: 235118 [Multi-domain] Cd Length: 454 Bit Score: 248.70 E-value: 6.29e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 1 MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERrGVISTANYPARKFGVRSAMPTGMALKLCPHLT-LLPGRFDAY 79
Cdd:PRK03348 4 QRWVLHLDMDAFFASVEQLTRPTLRGRPVLVGGLGGR-GVVAGASYEARVFGARSAMPMHQARRLVGNGAvVLPPRFVVY 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 80 KEASNHIREIFSRYTSRIEPLSLDEAYLD----VTDSVhchGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDM 155
Cdd:PRK03348 83 RAASRRVFDTLRELSPVVEQLSFDEAFVEpaelAGASA---EEVEAFAERLRARVREETGLPASVGAGSGKQIAKIASGL 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 156 NKPNGQFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKC---DLVMLLKRfgKFGRILWERSQGIDER 232
Cdd:PRK03348 160 AKPDGIRVVPPGEERELLAPLPVRRLWGIGPVTEEKLHRLGIETIGDLAALseaEVANLLGA--TVGPALHRLARGIDDR 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 233 DVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKvkpDLLIARQ-GVKLKFDDFQQTTQEHV--WPRLNK 309
Cdd:PRK03348 238 PVAERAEAKQISAESTFAVDLTTRAQLREAIERIAEHAHRRLLK---DGRGARTvTVKLRKSDFSTLTRSATlpYATDDA 314
|
330 340
....*....|....*....|....
gi 16128217 310 ADLIATARKTWDERRGGRGVRLVG 333
Cdd:PRK03348 315 AVLAATARRLLLDPDEIGPIRLVG 338
|
|
| PRK03858 |
PRK03858 |
DNA polymerase IV; Validated |
4-349 |
1.36e-77 |
|
DNA polymerase IV; Validated
Pssm-ID: 179663 [Multi-domain] Cd Length: 396 Bit Score: 243.36 E-value: 1.36e-77
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 4 IIHVDMDCFFAAVEMRDNPALRDIPIAIGGsrerrGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEAS 83
Cdd:PRK03858 6 ILHADLDSFYASVEQRDDPALRGRPVIVGG-----GVVLAASYEAKAYGVRTAMGGRQARRLCPQAVVVPPRMSAYSRAS 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 84 NHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQFV 163
Cdd:PRK03858 81 KAVFEVFRDTTPLVEGLSIDEAFLDVGGLRRISGTPVQIAARLRRRVREEVGLPITVGVARTKFLAKVASQVAKPDGLLV 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 164 ITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGK-FGRILWERSQGIDERDVNSERLRKS 242
Cdd:PRK03858 161 VPPDRELAFLHPLPVRRLWGVGPVTAAKLRAHGITTVGDVAELPESALVSLLGPaAGRHLHALAHNRDPRRVETGRRRRS 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 243 VGVERTMAEDIHHWSECEAIIERLYPELERRLAKVkpdlliARQG----VKLKFDDFQQTTQEHV--WPRLNKADLIATA 316
Cdd:PRK03858 241 VGAQRALGRGPNSPAEVDAVVVALVDRVARRMRAA------GRTGrtvvLRLRFDDFTRATRSHTlpRPTASTATLLAAA 314
|
330 340 350
....*....|....*....|....*....|....*.
gi 16128217 317 RKTWDERR---GGRGVRLVGLHVTLLDPQMERQLVL 349
Cdd:PRK03858 315 RDLVAAAApliAERGLTLVGFAVSNLDDDGAQQLEL 350
|
|
| PRK03103 |
PRK03103 |
DNA polymerase IV; Reviewed |
1-349 |
3.46e-72 |
|
DNA polymerase IV; Reviewed
Pssm-ID: 235104 [Multi-domain] Cd Length: 409 Bit Score: 229.89 E-value: 3.46e-72
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 1 MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERR-GVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAY 79
Cdd:PRK03103 2 ERVILLVDMQSFYASVEKAANPELKGRPVIVSGDPERRsGVVLAACPLAKAYGVKTAERLWEAQQKCPDLVVVKPRMQRY 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 80 KEASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDM---N 156
Cdd:PRK03103 82 IDVSLQITRILEDFTDLVEPFSIDEQFLDVTGSQKLFGSPLEIAQKIQQRIMRETGVYARVGIGPNKLLAKMACDNfakK 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 157 KPNGQFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFGRILWERSQGIDERDVNS 236
Cdd:PRK03103 162 NPDGLFTLDKEDVPADLWPLPVRKLFGVGSRMEKHLRRMGIRTIGQLANTPLERLKKRWGINGEVLWRTANGIDYSPVTP 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 237 ERL--RKSVGVERTMAEDIHHWSECEAIIERLYPELERRlakvkpdlliARQG--------VKLKFDDFQQTTQEHVWPR 306
Cdd:PRK03103 242 HSLdrQKAIGHQMTLPRDYRGFEEIKVVLLELCEEVCRR----------ARAKgymgrtvsVSLRGADFDWPTGFSRQMT 311
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 16128217 307 LNK-----ADLIATARKTWDERRGGRGVRLVGLHVTLLDPQMERQLVL 349
Cdd:PRK03103 312 LPEptnlaMEVYEAACKLFHRHWDGKPVRRVGVTLSNLVSDDVWQLSL 359
|
|
| PolY |
cd00424 |
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases ... |
6-274 |
3.83e-67 |
|
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Most TLS polymerases are members of the Y-family, including Pol eta, Pol kappa/IV, Pol iota, Rev1, and Pol V, which is found exclusively in bacteria. In eukaryotes, the B-family polymerase Pol zeta also functions as a TLS polymerase. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176453 [Multi-domain] Cd Length: 343 Bit Score: 214.92 E-value: 3.83e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 6 HVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNH 85
Cdd:cd00424 2 HIDFDNFFASVEQLARPELKGRPVVVVPFNSDSTCVIACSYEARKYGVKRGMPVREARKMCPNLILVPARLDLYRRLSER 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 86 IREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNEL-QLTASAGVAPVKFLAKIASDMNKPNGQFVI 164
Cdd:cd00424 82 LLSELEEVAPLVEVASIDELFLDLTGSARLLGLGSEVALRIKRHIAEQLgGITASIGIASNKLLAKLAAKYAKPDGLTIL 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 165 TPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLV-MLLKRFGKFGRILWERSQGIDERDVNSERLRKSV 243
Cdd:cd00424 162 DPEDLPGFLSKLPLTDLPGIGAVTAKRLEAVGINPIGDLLAASPDaLLALWGGVSGERLWYALRGIDDEPLSPPRPRKSF 241
|
250 260 270
....*....|....*....|....*....|.
gi 16128217 244 GVERTMAEDIHHWSECEAIIERLYPELERRL 274
Cdd:cd00424 242 SHERVLPRDSRNAEDARPLLRLLLEKLARRL 272
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
7-154 |
3.49e-64 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 200.49 E-value: 3.49e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 7 VDMDCFFAAVEMRDNPALRDIPIAIGGSReRRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHI 86
Cdd:pfam00817 1 IDMDAFFASVELLRDPELKGKPVAVGGGN-GRGIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVVPPDLELYRRASRKI 79
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 16128217 87 REIFSRYTS-RIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASD 154
Cdd:pfam00817 80 FEILRRFSTpKVEQASIDEAFLDLTGLEKLFGAEEALAKRLRREIAEETGLTCSIGIAPNKLLAKLASD 148
|
|
| PRK01216 |
PRK01216 |
DNA polymerase IV; Validated |
4-217 |
2.06e-56 |
|
DNA polymerase IV; Validated
Pssm-ID: 179251 [Multi-domain] Cd Length: 351 Bit Score: 187.30 E-value: 2.06e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 4 IIHVDMDCFFAAVEMRDNPALRDIPIAI---GGSRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYK 80
Cdd:PRK01216 3 ILFVDFDYFFAQVEEVLNPSLKGKPVVVcvySGRFEDSGAVATANYEARKLGIKAGMPIVEAKKILPNAVYLPMRKEVYQ 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 81 EASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG 160
Cdd:PRK01216 83 QVSNRIMKLLREYSEKIEIASIDEAYLDISDKVKNYQDAYNLGLEIKNKILEKEKITVTVGISKNKVFAKIAADMAKPNG 162
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....*..
gi 16128217 161 QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGK 217
Cdd:PRK01216 163 IKVIDDEEVKRFINELDIADIPGIGDITAEKLKKLGVNKLVDTLRIEFDELKGIIGE 219
|
|
| PTZ00205 |
PTZ00205 |
DNA polymerase kappa; Provisional |
5-322 |
2.08e-54 |
|
DNA polymerase kappa; Provisional
Pssm-ID: 140232 [Multi-domain] Cd Length: 571 Bit Score: 187.53 E-value: 2.08e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 5 IHVDMDCFFAAVEMRDNPALRDIPIAIGgsreRRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASN 84
Cdd:PTZ00205 136 IHLDMDMFYAAVEIKKHPEYAAIPLAIG----TMTMLQTANYVARGRGIRQGMPGFLALKICPNLLILPPDFDAYNEESN 211
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 85 HIREIFSRYTSRIEPLSLDEAYLDVTDSVH-CHGSATL--IAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQ 161
Cdd:PTZ00205 212 TVRRIVAEYDPNYISFGLDELTLEVSAYIErFEGTKTAedVASELRVRVFGETKLTASAGIGPTAALAKIASNINKPNGQ 291
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 162 FVI---TPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDV--QKCDLVMLLKRfgKFGRILWERSQGIDE----- 231
Cdd:PTZ00205 292 HDLnlhTRGDVMTYVRDLGLRSVPGVGKVTEALLKGLGITTLSDIynRRVELCYILHN--NLFRFLLGASIGIMQwpdaa 369
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 232 RDVNSERL-------RKSVGVERTM-----AEDIHHWseCEAIIERLYPELErrlakvKPDLLIARQGVKLKFDDFQQtt 299
Cdd:PTZ00205 370 TAANTENCegatggqRKAISSERSFttprtKEGLQEM--VDTVFNGAYEEMR------KSELMCRQISLTIRWASYRY-- 439
|
330 340
....*....|....*....|...
gi 16128217 300 QEHVWPRLNKADLIATARKTWDE 322
Cdd:PTZ00205 440 QQYTKSLIQYSDDSATLRRAVDG 462
|
|
| PolY_Pol_eta |
cd01702 |
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. ... |
6-276 |
1.56e-52 |
|
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Unlike other Y-family members, Pol eta can efficiently and accurately replicate DNA past UV-induced lesions. Its activity is initiated by two simultaneous interactions: the PIP box in pol eta interacting with PCNA, and the UBZ (ubiquitin-binding zinc finger) in pol eta interacting with monoubiquitin attached to PCNA. Pol eta is more efficient in copying damaged DNA than undamaged DNA and seems to recognize when a lesion has been passed, facilitating a lesion-dependent dissociation from the DNA.
Pssm-ID: 176456 [Multi-domain] Cd Length: 359 Bit Score: 177.51 E-value: 1.56e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 6 HVDMDCFFAAVEMRDNPALRDIPIAIggsRERRGVISTaNYPARKFGVRSAMPTGMALKLCPHLTL-------------- 71
Cdd:cd01702 2 HIDMDAFFAQVEQVRLGLLRNDPVAV---VQWNSIIAV-SYAARAFGVTRFMTIDEAKKKCPDLILahvatykkgedead 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 72 -----LPGRF----DAYKEASNHIREIFSRYTSRIEPLSLDEAYLDVtdsvhchGSAtlIAQEIRQTIFNELQLTASAGV 142
Cdd:cd01702 78 yhenpSPARHkvslDPYRRASRKILNILKRFGDVVEKASIDEAYLDL-------GSR--IVEEIRQQVYDELGYTCSAGI 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 143 APVKFLAKIASDMNKPNGQFVITPAEVPAFLQTLPLAKIPGV-GKVSAAKLEAMGLRTCGDVQKCDLVMLL---KRFGKF 218
Cdd:cd01702 149 AHNKMLAKLASGMNKPNAQTILRNDAVASFLSSLPITSIRGLgGKLGEEIIDLLGLPTEGDVAGFRSSESDlqeHFGEKL 228
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 16128217 219 GRILWERSQGIDERDVNSERLRKSVGVERTMA-------EDIHHWseceaiIERLYPELERRLAK 276
Cdd:cd01702 229 GEWLYNLLRGIDHEPVKPRPLPKSMGSSKNFPgktalstEDVQHW------LLVLASELNSRLED 287
|
|
| PRK03352 |
PRK03352 |
DNA polymerase IV; Validated |
1-341 |
8.28e-50 |
|
DNA polymerase IV; Validated
Pssm-ID: 179564 [Multi-domain] Cd Length: 346 Bit Score: 169.82 E-value: 8.28e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 1 MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGS---RERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFD 77
Cdd:PRK03352 4 PRWVLHVDLDQFIAAVELLRRPELAGLPVIVGGNgdpTEPRKVVTCASYEARAFGVRAGMPLRTAARRCPDAVFLPSDPA 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 78 AYKEASNHIREIFSRYTSRIEPLSLDEAYLDVTDsvhchGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNK 157
Cdd:PRK03352 84 AYDAASEEVMATLRDLGVPVEVWGWDEAFLGVDT-----DDPEALAEEIRAAVLERTGLSCSVGIGDNKLRAKIATGFAK 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 158 PNGQFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFG-KFGRILWERSQGIDERDVNS 236
Cdd:PRK03352 159 PAGVFRLTDANWMAVMGDRPTDALWGVGPKTAKRLAALGITTVADLAAADPAELAATFGpTTGPWLLLLARGGGDTEVSA 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 237 E-RLRKSVGVERTMAEDIHHWSECEAIIERLypeLERRLAKV-KPDLLIARQGVKLKFDDFqqTTQEHVW----PRLNKA 310
Cdd:PRK03352 239 EpWVPRSRSREVTFPQDLTDRAEVESAVREL---ARRVLDEVvAEGRPVTRVAVKVRTATF--YTRTKIRklpePTTDPD 313
|
330 340 350
....*....|....*....|....*....|.
gi 16128217 311 DLIATARKTWDERRGGRGVRLVGLHVTLLDP 341
Cdd:PRK03352 314 VIEAAALDVLDRFELDRPVRLLGVRLELAMP 344
|
|
| PolY_Pol_iota |
cd01703 |
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. ... |
5-274 |
3.90e-49 |
|
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.
Pssm-ID: 176457 [Multi-domain] Cd Length: 379 Bit Score: 169.19 E-value: 3.90e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 5 IHVDMDCFFAAVEMRDNPALRDIPIAIggsrERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGR-FDAYKEAS 83
Cdd:cd01703 1 IHLDLDCFYAQVEEIRDPSLKSKPLGI----QQKYIVVTCNYEARRLGVKKLMSIKDAKEICPDLVLVNGEdLTPFRDMS 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 84 NHIREIFSRYT--SRIEPLSLDEAYLDVTDSVHCHGSAtlIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQ 161
Cdd:cd01703 77 KKVYRLLRSYSwnDRVERLGFDENFMDVTEMRLLVASH--IAYEMRERIENELGLTCCAGIASNKLLAKLVGSVNKPNQQ 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 162 FVITP---AEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCD---------------LVMLLKRFGK-FGRIL 222
Cdd:cd01703 155 TTLLPpscADLMDFMDLHDLRKIPGIGYKTAAKLEAHGISSVRDLQEFSnrnrqtvgaapslleLLLMVKEFGEgIGQRI 234
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|....
gi 16128217 223 WERSQGIDERDVNSER-LRKSVGVERTMAE-DIHHWSECEAIIERLYPELERRL 274
Cdd:cd01703 235 WKLLFGRDTSPVKPASdFPQQISIEDSYKKcSLEEIREARNKIEELLASLLERM 288
|
|
| PolY_Pol_V_umuC |
cd01700 |
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion ... |
7-264 |
1.75e-40 |
|
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion synthesis (TLS) polymerase that consists of the heterotrimer of one umuC and two umuD subunits. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol V, RecA, single stranded DNA-binding protein, beta sliding clamp, and gamma clamp loading complex are responsible for inducing the SOS response in bacteria to repair UV-induced DNA damage.
Pssm-ID: 176454 [Multi-domain] Cd Length: 344 Bit Score: 145.38 E-value: 1.75e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 7 VDMDCFFAAVEMRDNPALRDIPIAIGgSRERRGVIStANYPARKFGVrsamPTGMALKLCPHL------TLLPGRFDAYK 80
Cdd:cd01700 3 VDCNSFYASCERVFRPLLLGRPLVVL-SNNDGCVIA-RSPEAKALGI----KMGSPYFKVPDLlerhgvAVFSSNYALYG 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 81 EASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHChGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDM----N 156
Cdd:cd01700 77 DMSRRIMSILERFSPDVEVYSIDESFLDLTGSLRF-GDLEELARKIRRRILQETGIPVTVGIGPTKTLAKLANDLakkkN 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 157 KPNGQFVIT-PAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFGRILWERSQGIDERDV- 234
Cdd:cd01700 156 PYGGVVDLTdEEVRDKLLKILPVGDVWGIGRRTAKKLNAMGIHTAGDLAQADPDLLRKKFGVVGERLVRELNGIDCLPLe 235
|
250 260 270
....*....|....*....|....*....|.
gi 16128217 235 NSERLRKSVGVERTMAEDIHHWSE-CEAIIE 264
Cdd:cd01700 236 EYPPPKKSIGSSRSFGRDVTDLDElKQALAE 266
|
|
| PolY_like |
cd03468 |
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that ... |
19-336 |
1.51e-27 |
|
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176458 [Multi-domain] Cd Length: 335 Bit Score: 110.55 E-value: 1.51e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 19 RDNPALRDIPIAIGgSRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIE 98
Cdd:cd03468 15 RNRPADDEAPLAVV-ERKKAGRILACNAAARAAGVRPGMPLAEALALCPNLQVVEYDPEADARALQELALWLLRFTPLVA 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 99 PLSLDEAYLDVTDSVHCHGSATLIAQEIRQTiFNELQLTASAGVAPVKFLAKIASDMNKPNGqfvITPAEVPAFLQTL-- 176
Cdd:cd03468 94 LDGPDGLLLDVTGCLHLFGGEDALAASLRAA-LATLGLSARAGIADTPGAAWLLARAGGGRG---VLRREALAAALVLla 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 177 -PLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFGRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHH 255
Cdd:cd03468 170 pLPVAALRLPPETVELLARLGLRTLGDLAALPRAELARRFGLALLLRLDQAYGRDPEPLLFSPPPPAFDFRLELQLEEPI 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 256 WSECEAIIERLYPELERRLAkvKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATAR-----KTWDERRGGRGVR 330
Cdd:cd03468 250 ARGLLFPLRRLLEQLCAFLA--LRGLGARRLSLTLFREDGRVTRVLVGLARPSRDDLPLLRLlrerlERLALPRGIAPVR 327
|
....*.
gi 16128217 331 LVGLHV 336
Cdd:cd03468 328 LLALTA 333
|
|
| umuC |
PRK03609 |
translesion error-prone DNA polymerase V subunit UmuC; |
6-215 |
1.73e-15 |
|
translesion error-prone DNA polymerase V subunit UmuC;
Pssm-ID: 179607 [Multi-domain] Cd Length: 422 Bit Score: 77.11 E-value: 1.73e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 6 HVDMDCFFAAVEMRDNPALRDIPIAIGGSRErrGVISTANYPARKFGVRSAMP--TGMALKLCPHLTLLPGRFDAYKEAS 83
Cdd:PRK03609 4 LCDVNSFYASCETVFRPDLRGKPVVVLSNND--GCVIARSAEAKALGIKMGDPwfKQKDLFRRCGVVCFSSNYELYADMS 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 84 NHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHgSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKP-NGQF 162
Cdd:PRK03609 82 NRVMSTLEELSPRVEIYSIDEAFCDLTGVRNCR-DLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKwQRQT 160
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....*...
gi 16128217 163 -----VITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRF 215
Cdd:PRK03609 161 ggvvdLSNLERQRKLLSLQPVEEVWGVGRRISKKLNAMGIKTALDLADTNIRFIRKHF 218
|
|
| IMS_C |
pfam11799 |
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss). |
240-342 |
1.90e-13 |
|
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss).
Pssm-ID: 463354 [Multi-domain] Cd Length: 104 Bit Score: 65.66 E-value: 1.90e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128217 240 RKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKpdlLIARQ-GVKLKFDDFQQTTQEHVWPRL--NKADLIATA 316
Cdd:pfam11799 1 RKSIGAERTFGRDLTDLEELREALLELAEELAERLRRQG---LVARTvTVKIRYSDFRTITRSVTLPSPtdDTDEIYRAA 77
|
90 100
....*....|....*....|....*.
gi 16128217 317 RKTWDERRGGRGVRLVGLHVTLLDPQ 342
Cdd:pfam11799 78 LRLLRRLYRGRPVRLLGVSLSNLVPE 103
|
|
| IMS_HHH |
pfam11798 |
IMS family HHH motif; These proteins are involved in UV protection, eg. |
166-197 |
4.27e-07 |
|
IMS family HHH motif; These proteins are involved in UV protection, eg.
Pssm-ID: 432081 [Multi-domain] Cd Length: 32 Bit Score: 45.85 E-value: 4.27e-07
10 20 30
....*....|....*....|....*....|..
gi 16128217 166 PAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGL 197
Cdd:pfam11798 1 PDDVPEFLWPLPISKIPGIGKKLAEKLKALGI 32
|
|
| |
