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Conserved domains on  [gi|15236257|ref|NP_192225|]
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B3 domain protein [Arabidopsis thaliana]

Protein Classification

B3 domain-containing protein( domain architecture ID 10178069)

B3 domain-containing protein similar to Arabidopsis thaliana transcription factors: VAL3, FUS3, and LEC2; B3 domain-containing protein such as Arabidopsis thaliana transcription factors, VAL3, FUS3, and LEC2; B3 domain-containing protein such as Arabidopsis thaliana transcription factors, VAL3, FUS3, and LEC2

Gene Ontology:  GO:0003677
PubMed:  25002525|19503786|21155018|18986826

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
 69-158 1.04e-10

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


:

Pssm-ID: 197383  Cd Length: 98  Bit Score: 55.80  E-value: 1.04e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236257  69 LMDSDVkDNQYRLMLGKEPVKKMMDalgkteklGTKGLNVSVYGPNGENHKMVLKIWIK-GTPVLTSGWKNFVKSYKLEK 147
Cdd:cd10017   7 LTPSDV-SSLGRLVLPKKFAKAHLP--------PKEGVEVTLEDPDGKKWTVKLKKRKNsGRMVLSGGWKEFVRANGLKE 77

                        90
                ....*....|.
gi 15236257 148 HvDFLTIWMFR 158
Cdd:cd10017  78 G-DFLVFELDG 87
 
Name Accession Description Interval E-value
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
 69-158 1.04e-10

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 55.80  E-value: 1.04e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236257  69 LMDSDVkDNQYRLMLGKEPVKKMMDalgkteklGTKGLNVSVYGPNGENHKMVLKIWIK-GTPVLTSGWKNFVKSYKLEK 147
Cdd:cd10017   7 LTPSDV-SSLGRLVLPKKFAKAHLP--------PKEGVEVTLEDPDGKKWTVKLKKRKNsGRMVLSGGWKEFVRANGLKE 77

                        90
                ....*....|.
gi 15236257 148 HvDFLTIWMFR 158
Cdd:cd10017  78 G-DFLVFELDG 87
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
 108-169 1.51e-04

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 39.20  E-value: 1.51e-04
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 15236257    108 VSVYGPNGENHKMVLKIW-IKGTPVLTSGWKNFVKSYKLEKHvDFLTiwmFRHKKTREICFAI 169
Cdd:smart01019  35 ITLLDPDGKSWKVKLKYRgSSGMFLLTGGWKEFVKANGLKAG-DSLT---FELDGNGEFVVSI 93
At2g31720-like pfam03754
B3 domain-containing protein At2g31720-like; This entry represents a group of uncharacterized ...
 56-141 5.27e-03

B3 domain-containing protein At2g31720-like; This entry represents a group of uncharacterized plant proteins, including B3 domain-containing protein At2g31720 (also known as Protein AUXIN RESPONSE FACTOR 70) from Arabidopsis. These are DNA-binding proteins likely to be involved in stress response.


Pssm-ID: 281714  Cd Length: 113  Bit Score: 35.41  E-value: 5.27e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236257    56 NENKGKKRKVAEQLMDSDVKDNQYRLMLgkePVKKM--MDALG----------KTEKLGTKGLNVSVYGPNGENHKMVLK 123
Cdd:pfam03754  15 NGAEDPKLIIEKTLFKTDVDPTQSRLSM---PFNQLirNDFLTeeelriireeDKIKDRDIGVGVILVDQESKKWVLKLK 91

                          90       100
                  ....*....|....*....|.
gi 15236257   124 IW-IKGTP--VLTSGWKNFVK 141
Cdd:pfam03754  92 KWdMKGTWnyVLVCGWNDVVT 112
 
Name Accession Description Interval E-value
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
 69-158 1.04e-10

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 55.80  E-value: 1.04e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236257  69 LMDSDVkDNQYRLMLGKEPVKKMMDalgkteklGTKGLNVSVYGPNGENHKMVLKIWIK-GTPVLTSGWKNFVKSYKLEK 147
Cdd:cd10017   7 LTPSDV-SSLGRLVLPKKFAKAHLP--------PKEGVEVTLEDPDGKKWTVKLKKRKNsGRMVLSGGWKEFVRANGLKE 77

                        90
                ....*....|.
gi 15236257 148 HvDFLTIWMFR 158
Cdd:cd10017  78 G-DFLVFELDG 87
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
 108-169 1.51e-04

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 39.20  E-value: 1.51e-04
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 15236257    108 VSVYGPNGENHKMVLKIW-IKGTPVLTSGWKNFVKSYKLEKHvDFLTiwmFRHKKTREICFAI 169
Cdd:smart01019  35 ITLLDPDGKSWKVKLKYRgSSGMFLLTGGWKEFVKANGLKAG-DSLT---FELDGNGEFVVSI 93
At2g31720-like pfam03754
B3 domain-containing protein At2g31720-like; This entry represents a group of uncharacterized ...
 56-141 5.27e-03

B3 domain-containing protein At2g31720-like; This entry represents a group of uncharacterized plant proteins, including B3 domain-containing protein At2g31720 (also known as Protein AUXIN RESPONSE FACTOR 70) from Arabidopsis. These are DNA-binding proteins likely to be involved in stress response.


Pssm-ID: 281714  Cd Length: 113  Bit Score: 35.41  E-value: 5.27e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236257    56 NENKGKKRKVAEQLMDSDVKDNQYRLMLgkePVKKM--MDALG----------KTEKLGTKGLNVSVYGPNGENHKMVLK 123
Cdd:pfam03754  15 NGAEDPKLIIEKTLFKTDVDPTQSRLSM---PFNQLirNDFLTeeelriireeDKIKDRDIGVGVILVDQESKKWVLKLK 91

                          90       100
                  ....*....|....*....|.
gi 15236257   124 IW-IKGTP--VLTSGWKNFVK 141
Cdd:pfam03754  92 KWdMKGTWnyVLVCGWNDVVT 112
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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