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Conserved domains on  [gi|15231105|ref|NP_191427|]
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multiprotein bridging factor 1B [Arabidopsis thaliana]

Protein Classification

EDF1/MBF1 family transcription factor( domain architecture ID 12094462)

EDF1/MBF1 family transcription factor such as vertebrate endothelial differentiation-related factor (EDF1) and multiprotein bridging factor 1 (MBF1), which function as transcriptional coactivators

Gene Ontology:  GO:0003677
PubMed:  2644244|15808743

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MBF1 pfam08523
Multiprotein bridging factor 1; This domain is found in the multiprotein bridging factor 1 ...
 10-77 1.04e-23

Multiprotein bridging factor 1; This domain is found in the multiprotein bridging factor 1 (MBF1) which forms a heterodimer with MBF2. It has been shown to make direct contact with the TATA-box binding protein (TBP) and interacts with Ftz-F1, stabilising the Ftz-F1-DNA complex. It is also found in the endothelial differentiation-related factor (EDF-1). Human EDF-1 is involved in the repression of endothelial differentiation, interacts with CaM and is phosphorylated by PKC. The domain is found in a wide range of eukaryotic proteins including metazoans, fungi and plants. A helix-turn-helix motif (pfam01381) is found to its C-terminus.


:

Pssm-ID: 462508  Cd Length: 68  Bit Score: 87.30  E-value: 1.04e-23
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15231105    10 DWEPVV-IRKRAPNAAAKRDEKTVNAARRSGADIETVRKFNAGSNKaASSGTSLNTKKLDDDTENLSHD 77
Cdd:pfam08523   1 DWDSVTvIGKKAPRATVARSESALNAARRSGAVVETEKKFYAGTNK-AHHGEGQNTAKLDRETEELKHE 68
aMBF1 COG1813
Archaeal ribosome-binding protein aMBF1, putative translation factor, contains Zn-ribbon and ...
 78-138 4.03e-12

Archaeal ribosome-binding protein aMBF1, putative translation factor, contains Zn-ribbon and HTH domains [Translation, ribosomal structure and biogenesis];


:

Pssm-ID: 441418 [Multi-domain]  Cd Length: 70  Bit Score: 57.64  E-value: 4.03e-12
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15231105  78 RVPTELKKAIMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALGAKL 138
Cdd:COG1813   8 ELVEDYGERIREAREARGLSQEELAEKLGVSESTIRRIERGEATPSLDTLRKLEKALGISL 68
 
Name Accession Description Interval E-value
MBF1 pfam08523
Multiprotein bridging factor 1; This domain is found in the multiprotein bridging factor 1 ...
 10-77 1.04e-23

Multiprotein bridging factor 1; This domain is found in the multiprotein bridging factor 1 (MBF1) which forms a heterodimer with MBF2. It has been shown to make direct contact with the TATA-box binding protein (TBP) and interacts with Ftz-F1, stabilising the Ftz-F1-DNA complex. It is also found in the endothelial differentiation-related factor (EDF-1). Human EDF-1 is involved in the repression of endothelial differentiation, interacts with CaM and is phosphorylated by PKC. The domain is found in a wide range of eukaryotic proteins including metazoans, fungi and plants. A helix-turn-helix motif (pfam01381) is found to its C-terminus.


Pssm-ID: 462508  Cd Length: 68  Bit Score: 87.30  E-value: 1.04e-23
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15231105    10 DWEPVV-IRKRAPNAAAKRDEKTVNAARRSGADIETVRKFNAGSNKaASSGTSLNTKKLDDDTENLSHD 77
Cdd:pfam08523   1 DWDSVTvIGKKAPRATVARSESALNAARRSGAVVETEKKFYAGTNK-AHHGEGQNTAKLDRETEELKHE 68
aMBF1 COG1813
Archaeal ribosome-binding protein aMBF1, putative translation factor, contains Zn-ribbon and ...
 78-138 4.03e-12

Archaeal ribosome-binding protein aMBF1, putative translation factor, contains Zn-ribbon and HTH domains [Translation, ribosomal structure and biogenesis];


Pssm-ID: 441418 [Multi-domain]  Cd Length: 70  Bit Score: 57.64  E-value: 4.03e-12
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15231105  78 RVPTELKKAIMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALGAKL 138
Cdd:COG1813   8 ELVEDYGERIREAREARGLSQEELAEKLGVSESTIRRIERGEATPSLDTLRKLEKALGISL 68
HTH_XRE smart00530
Helix-turn-helix XRE-family like proteins;
87-139 2.88e-11

Helix-turn-helix XRE-family like proteins;


Pssm-ID: 197775 [Multi-domain]  Cd Length: 56  Bit Score: 54.83  E-value: 2.88e-11
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|...
gi 15231105     87 IMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALGAKLR 139
Cdd:smart00530   2 LKELREEKGLTQEELAEKLGVSRSTLSRIENGKRKPSLETLKKLAKALGVSLD 54
HTH_3 pfam01381
Helix-turn-helix; This large family of DNA binding helix-turn helix proteins includes Cro and ...
 87-139 8.53e-10

Helix-turn-helix; This large family of DNA binding helix-turn helix proteins includes Cro and CI. Within the protein Swiss:Q5F9C2, the full protein fold incorporates a helix-turn-helix motif, but the function of this member is unlikely to be that of a DNA-binding regulator, the function of most other members, so is not necessarily characteriztic of the whole family.


Pssm-ID: 460181 [Multi-domain]  Cd Length: 55  Bit Score: 51.00  E-value: 8.53e-10
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 15231105    87 IMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALGAKLR 139
Cdd:pfam01381   1 LKELREELGLSQEELAEKLGVSRSTISKIENGKREPSLETLKKLAEALGVSLD 53
HTH_XRE cd00093
Helix-turn-helix XRE-family like proteins. Prokaryotic DNA binding proteins belonging to the ...
 85-135 1.53e-09

Helix-turn-helix XRE-family like proteins. Prokaryotic DNA binding proteins belonging to the xenobiotic response element family of transcriptional regulators.


Pssm-ID: 238045 [Multi-domain]  Cd Length: 58  Bit Score: 50.63  E-value: 1.53e-09
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 15231105  85 KAIMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALG 135
Cdd:cd00093   2 ERLKELRKEKGLTQEELAEKLGVSRSTISRIENGKRNPSLETLEKLAKALG 52
TIGR00270 TIGR00270
TIGR00270 family protein; [Hypothetical proteins, Conserved]
 87-141 1.10e-04

TIGR00270 family protein; [Hypothetical proteins, Conserved]


Pssm-ID: 129371 [Multi-domain]  Cd Length: 154  Bit Score: 39.89  E-value: 1.10e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 15231105    87 IMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALGAKLRGK 141
Cdd:TIGR00270  74 IRREREKRGWSQEQLAKKIQEKESLIKKIENAEIEPEPKVVEKLEKLLKIKLREQ 128
PRK13355 PRK13355
bifunctional HTH-domain containing protein/aminotransferase; Provisional
 89-135 5.11e-03

bifunctional HTH-domain containing protein/aminotransferase; Provisional


Pssm-ID: 237361 [Multi-domain]  Cd Length: 517  Bit Score: 35.87  E-value: 5.11e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 15231105   89 QARGEKKLTQSQLAHLINE------KPQVIQeYESGKAIPNQQILSKLERALG 135
Cdd:PRK13355  10 QAMKARGLKQEDLVHAAEArgvklgKSHISQ-YVSGKTGPRRDVLPFLAAILG 61
 
Name Accession Description Interval E-value
MBF1 pfam08523
Multiprotein bridging factor 1; This domain is found in the multiprotein bridging factor 1 ...
 10-77 1.04e-23

Multiprotein bridging factor 1; This domain is found in the multiprotein bridging factor 1 (MBF1) which forms a heterodimer with MBF2. It has been shown to make direct contact with the TATA-box binding protein (TBP) and interacts with Ftz-F1, stabilising the Ftz-F1-DNA complex. It is also found in the endothelial differentiation-related factor (EDF-1). Human EDF-1 is involved in the repression of endothelial differentiation, interacts with CaM and is phosphorylated by PKC. The domain is found in a wide range of eukaryotic proteins including metazoans, fungi and plants. A helix-turn-helix motif (pfam01381) is found to its C-terminus.


Pssm-ID: 462508  Cd Length: 68  Bit Score: 87.30  E-value: 1.04e-23
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15231105    10 DWEPVV-IRKRAPNAAAKRDEKTVNAARRSGADIETVRKFNAGSNKaASSGTSLNTKKLDDDTENLSHD 77
Cdd:pfam08523   1 DWDSVTvIGKKAPRATVARSESALNAARRSGAVVETEKKFYAGTNK-AHHGEGQNTAKLDRETEELKHE 68
aMBF1 COG1813
Archaeal ribosome-binding protein aMBF1, putative translation factor, contains Zn-ribbon and ...
 78-138 4.03e-12

Archaeal ribosome-binding protein aMBF1, putative translation factor, contains Zn-ribbon and HTH domains [Translation, ribosomal structure and biogenesis];


Pssm-ID: 441418 [Multi-domain]  Cd Length: 70  Bit Score: 57.64  E-value: 4.03e-12
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15231105  78 RVPTELKKAIMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALGAKL 138
Cdd:COG1813   8 ELVEDYGERIREAREARGLSQEELAEKLGVSESTIRRIERGEATPSLDTLRKLEKALGISL 68
HTH_XRE smart00530
Helix-turn-helix XRE-family like proteins;
87-139 2.88e-11

Helix-turn-helix XRE-family like proteins;


Pssm-ID: 197775 [Multi-domain]  Cd Length: 56  Bit Score: 54.83  E-value: 2.88e-11
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|...
gi 15231105     87 IMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALGAKLR 139
Cdd:smart00530   2 LKELREEKGLTQEELAEKLGVSRSTLSRIENGKRKPSLETLKKLAKALGVSLD 54
HTH_3 pfam01381
Helix-turn-helix; This large family of DNA binding helix-turn helix proteins includes Cro and ...
 87-139 8.53e-10

Helix-turn-helix; This large family of DNA binding helix-turn helix proteins includes Cro and CI. Within the protein Swiss:Q5F9C2, the full protein fold incorporates a helix-turn-helix motif, but the function of this member is unlikely to be that of a DNA-binding regulator, the function of most other members, so is not necessarily characteriztic of the whole family.


Pssm-ID: 460181 [Multi-domain]  Cd Length: 55  Bit Score: 51.00  E-value: 8.53e-10
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 15231105    87 IMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALGAKLR 139
Cdd:pfam01381   1 LKELREELGLSQEELAEKLGVSRSTISKIENGKREPSLETLKKLAEALGVSLD 53
HTH_XRE cd00093
Helix-turn-helix XRE-family like proteins. Prokaryotic DNA binding proteins belonging to the ...
 85-135 1.53e-09

Helix-turn-helix XRE-family like proteins. Prokaryotic DNA binding proteins belonging to the xenobiotic response element family of transcriptional regulators.


Pssm-ID: 238045 [Multi-domain]  Cd Length: 58  Bit Score: 50.63  E-value: 1.53e-09
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 15231105  85 KAIMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALG 135
Cdd:cd00093   2 ERLKELRKEKGLTQEELAEKLGVSRSTISRIENGKRNPSLETLEKLAKALG 52
XRE COG1476
DNA-binding transcriptional regulator, XRE-family HTH domain [Transcription];
82-138 5.99e-08

DNA-binding transcriptional regulator, XRE-family HTH domain [Transcription];


Pssm-ID: 441085 [Multi-domain]  Cd Length: 68  Bit Score: 46.76  E-value: 5.99e-08
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 15231105  82 ELKKAIMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALGAKL 138
Cdd:COG1476   4 KLGNRLKELRKERGLTQEELAELLGVSRQTISAIENGKYNPSLELALKIARALGVSL 60
HipB COG1396
Transcriptional regulator, contains XRE-family HTH domain [Transcription];
77-135 6.68e-07

Transcriptional regulator, contains XRE-family HTH domain [Transcription];


Pssm-ID: 441006 [Multi-domain]  Cd Length: 83  Bit Score: 44.22  E-value: 6.68e-07
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*....
gi 15231105  77 DRVPTELKKAIMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALG 135
Cdd:COG1396   2 STLKKALGERLRELRKARGLTQEELAERLGVSRSTISRIERGRRNPSLETLLKLAKALG 60
TIGR00270 TIGR00270
TIGR00270 family protein; [Hypothetical proteins, Conserved]
 87-141 1.10e-04

TIGR00270 family protein; [Hypothetical proteins, Conserved]


Pssm-ID: 129371 [Multi-domain]  Cd Length: 154  Bit Score: 39.89  E-value: 1.10e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 15231105    87 IMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALGAKLRGK 141
Cdd:TIGR00270  74 IRREREKRGWSQEQLAKKIQEKESLIKKIENAEIEPEPKVVEKLEKLLKIKLREQ 128
couple_hipB TIGR03070
transcriptional regulator, y4mF family; Members of this family belong to a clade of ...
 81-138 3.98e-04

transcriptional regulator, y4mF family; Members of this family belong to a clade of helix-turn-helix DNA-binding proteins, among the larger family pfam01381 (HTH_3; Helix-turn-helix). Members are similar in sequence to the HipB protein of E. coli. Genes for members of the seed alignment for this protein family were found to be closely linked to genes encoding proteins related to HipA. The HibBA operon appears to have some features in common with toxin-antitoxin post-segregational killing systems. [Regulatory functions, DNA interactions]


Pssm-ID: 213767 [Multi-domain]  Cd Length: 58  Bit Score: 36.48  E-value: 3.98e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 15231105    81 TELKKAIMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALGAKL 138
Cdd:TIGR03070   1 KQIGILVRARRKALGLTQADLADLAGVGLRFIRDLENGKPTVRLDKVLRVLDALGLEL 58
AF2118 COG3620
Predicted transcriptional regulator, contains an XRE-type HTH domain (archaeal members contain ...
 71-140 6.94e-04

Predicted transcriptional regulator, contains an XRE-type HTH domain (archaeal members contain CBS pair) [Transcription];


Pssm-ID: 442838 [Multi-domain]  Cd Length: 95  Bit Score: 36.54  E-value: 6.94e-04
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15231105  71 TENLSHDRVPTELKKAIMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALGAKLRG 140
Cdd:COG3620   6 SRDVVTVSPDDTLGEALRLMRKELGLSQLPVAELVGVSQSDILRIESGKRDPTVSTLEKIAEALGKELSA 75
YiaG COG2944
DNA-binding transcriptional regulator YiaG, XRE-type HTH domain [Transcription];
87-131 2.61e-03

DNA-binding transcriptional regulator YiaG, XRE-type HTH domain [Transcription];


Pssm-ID: 442187 [Multi-domain]  Cd Length: 64  Bit Score: 34.52  E-value: 2.61e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*
gi 15231105  87 IMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLE 131
Cdd:COG2944  11 IRALRERLGLSQAEFAALLGVSVSTVRRWEQGRRKPSGAALKLLR 55
PRK13355 PRK13355
bifunctional HTH-domain containing protein/aminotransferase; Provisional
 89-135 5.11e-03

bifunctional HTH-domain containing protein/aminotransferase; Provisional


Pssm-ID: 237361 [Multi-domain]  Cd Length: 517  Bit Score: 35.87  E-value: 5.11e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 15231105   89 QARGEKKLTQSQLAHLINE------KPQVIQeYESGKAIPNQQILSKLERALG 135
Cdd:PRK13355  10 QAMKARGLKQEDLVHAAEArgvklgKSHISQ-YVSGKTGPRRDVLPFLAAILG 61
HTH_19 pfam12844
Helix-turn-helix domain; Members of this family contains a DNA-binding helix-turn-helix domain. ...
 85-135 9.04e-03

Helix-turn-helix domain; Members of this family contains a DNA-binding helix-turn-helix domain. This family contains many example antitoxins from bacterial toxin-antitoxin systems. These antitoxins are likely to be DNA-binding domains.


Pssm-ID: 463728 [Multi-domain]  Cd Length: 64  Bit Score: 33.03  E-value: 9.04e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 15231105    85 KAIMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQQILSKLERALG 135
Cdd:pfam12844   2 ERLRKAREERGLTQEELAERLGISRSQLSAIENGKSVPPAETLYKIAELLG 52
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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