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Conserved domains on  [gi|15225009|ref|NP_181435|]
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Acyl-CoA N-acyltransferases (NAT) superfamily protein [Arabidopsis thaliana]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 11441181)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
33-221 1.89e-21

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


:

Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 86.97  E-value: 1.89e-21
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15225009  33 RIRLATPSDVPFIHKLIHQmaVFERLTHLFSATESGLAS--TLFTSRPFQSFTVFLLEVsrspfpatitsspspdftpff 110
Cdd:COG1247   3 TIRPATPEDAPAIAAIYNE--AIAEGTATFETEPPSEEEreAWFAAILAPGRPVLVAEE--------------------- 59

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15225009 111 kthnldlpiddpesynfspdmlNDVVVaGFVLFFPnYSSFLSKPGFYIEDIFVREPYRRKGFGSMLLTAVAKQAVKMGYG 190
Cdd:COG1247  60 ----------------------DGEVV-GFASLGP-FRPRPAYRGTAEESIYVDPDARGRGIGRALLEALIERARARGYR 115

                       170       180       190
                ....*....|....*....|....*....|.
gi 15225009 191 RVEWVVLDWNVNAIKFYEQMGAQILQEWRVC 221
Cdd:COG1247 116 RLVAVVLADNEASIALYEKLGFEEVGTLPEV 146
 
Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
33-221 1.89e-21

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 86.97  E-value: 1.89e-21
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15225009  33 RIRLATPSDVPFIHKLIHQmaVFERLTHLFSATESGLAS--TLFTSRPFQSFTVFLLEVsrspfpatitsspspdftpff 110
Cdd:COG1247   3 TIRPATPEDAPAIAAIYNE--AIAEGTATFETEPPSEEEreAWFAAILAPGRPVLVAEE--------------------- 59

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15225009 111 kthnldlpiddpesynfspdmlNDVVVaGFVLFFPnYSSFLSKPGFYIEDIFVREPYRRKGFGSMLLTAVAKQAVKMGYG 190
Cdd:COG1247  60 ----------------------DGEVV-GFASLGP-FRPRPAYRGTAEESIYVDPDARGRGIGRALLEALIERARARGYR 115

                       170       180       190
                ....*....|....*....|....*....|.
gi 15225009 191 RVEWVVLDWNVNAIKFYEQMGAQILQEWRVC 221
Cdd:COG1247 116 RLVAVVLADNEASIALYEKLGFEEVGTLPEV 146
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 98-211 7.87e-18

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 76.40  E-value: 7.87e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15225009    98 ITSSPSPDFTPFFKTHNLDLPIDDPESYN-FSPDMLNDVVVAGFVLFFPNYSSFlskPGFYIEDIFVREPYRRKGFGSML 176
Cdd:pfam00583   4 LYELLSEEFPEPWPDEPLDLLEDWDEDASeGFFVAEEDGELVGFASLSIIDDEP---PVGEIEGLAVAPEYRGKGIGTAL 80

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 15225009   177 LTAVAKQAVKMGYGRVEWVVLDWNVNAIKFYEQMG 211
Cdd:pfam00583  81 LQALLEWARERGCERIFLEVAADNLAAIALYEKLG 115
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 132-193 3.90e-09

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 51.51  E-value: 3.90e-09
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15225009 132 LNDVVVAGFVLFFPNYSSflsKPGFYIEDIFVREPYRRKGFGSMLLTAVAKQAVKMGYGRVE 193
Cdd:cd04301   5 EDDGEIVGFASLSPDGSG---GDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLR 63
PRK09831 PRK09831
GNAT family N-acetyltransferase;
150-222 2.85e-04

GNAT family N-acetyltransferase;


Pssm-ID: 182099  Cd Length: 147  Bit Score: 39.94  E-value: 2.85e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15225009  150 FLSKPGFYIEDIFVREPYRRKGFGSMLLTAVAKQAVKMgygrvewvVLDWNVNAIKFYEQMGAQILQEWRV-CR 222
Cdd:PRK09831  67 FITCIEHYIDMLFVDPEYTRRGVASALLKPLIKSESEL--------TVDASITAKPFFERYGFQTVKQQRVeCR 132
 
Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
33-221 1.89e-21

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 86.97  E-value: 1.89e-21
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15225009  33 RIRLATPSDVPFIHKLIHQmaVFERLTHLFSATESGLAS--TLFTSRPFQSFTVFLLEVsrspfpatitsspspdftpff 110
Cdd:COG1247   3 TIRPATPEDAPAIAAIYNE--AIAEGTATFETEPPSEEEreAWFAAILAPGRPVLVAEE--------------------- 59

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15225009 111 kthnldlpiddpesynfspdmlNDVVVaGFVLFFPnYSSFLSKPGFYIEDIFVREPYRRKGFGSMLLTAVAKQAVKMGYG 190
Cdd:COG1247  60 ----------------------DGEVV-GFASLGP-FRPRPAYRGTAEESIYVDPDARGRGIGRALLEALIERARARGYR 115

                       170       180       190
                ....*....|....*....|....*....|.
gi 15225009 191 RVEWVVLDWNVNAIKFYEQMGAQILQEWRVC 221
Cdd:COG1247 116 RLVAVVLADNEASIALYEKLGFEEVGTLPEV 146
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 98-211 7.87e-18

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 76.40  E-value: 7.87e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15225009    98 ITSSPSPDFTPFFKTHNLDLPIDDPESYN-FSPDMLNDVVVAGFVLFFPNYSSFlskPGFYIEDIFVREPYRRKGFGSML 176
Cdd:pfam00583   4 LYELLSEEFPEPWPDEPLDLLEDWDEDASeGFFVAEEDGELVGFASLSIIDDEP---PVGEIEGLAVAPEYRGKGIGTAL 80

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 15225009   177 LTAVAKQAVKMGYGRVEWVVLDWNVNAIKFYEQMG 211
Cdd:pfam00583  81 LQALLEWARERGCERIFLEVAADNLAAIALYEKLG 115
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 139-217 1.02e-17

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 75.46  E-value: 1.02e-17
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15225009 139 GFVLFFPNYSsflsKPGFYIEDIFVREPYRRKGFGSMLLTAVAKQAVKMGYGRVEWVVLDWNVNAIKFYEQMGAQILQE 217
Cdd:COG0456   1 GFALLGLVDG----GDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGE 75
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 137-225 9.05e-17

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 73.93  E-value: 9.05e-17
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15225009 137 VAGFVLFFPnyssfLSKPGFYIEDIFVREPYRRKGFGSMLLTAVAKQAVKMGYGRVEWVVLDWNVNAIKFYEQMGAQILQ 216
Cdd:COG0454  45 PIGFAGLRR-----LDDKVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAAIRFYERLGFKEIE 119


                ....*....
gi 15225009 217 EWRVCRLTG 225
Cdd:COG0454 120 RYVAYVGGE 128
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
157-224 6.12e-12

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 59.54  E-value: 6.12e-12
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 15225009 157 YIEDIFVREPYRRKGFGSMLLTAVAKQAVKMGYGRVEWVVLDWNVNAIKFYEQMGAQILQEWRVCRLT 224
Cdd:COG3393  17 EISGVYTHPEYRGRGLASALVAALAREALARGARTPFLYVDADNPAARRLYERLGFRPVGEYATVLFR 84
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
137-220 2.46e-11

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 59.71  E-value: 2.46e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15225009 137 VAGFVLFFPNYSSFlSKPGFYIEDIFVREPYRRKGFGSMLLTAVAKQAVKMGYGRvewVVLDWNVNAIKFYEQMGAQILQ 216
Cdd:COG3153  50 IVGHVALSPVDIDG-EGPALLLGPLAVDPEYRGQGIGRALMRAALEAARERGARA---VVLLGDPSLLPFYERFGFRPAG 125


                ....
gi 15225009 217 EWRV 220
Cdd:COG3153 126 ELGL 129
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 133-211 2.07e-09

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 52.84  E-value: 2.07e-09
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15225009   133 NDVVVAGFVLFFPNYSSFlskpGFYIEDIFVREPYRRKGFGSMLLTAVAKQAVKMGYGRVEwvvLDWNVNAIKFYEQMG 211
Cdd:pfam13508  10 DDGKIVGFAALLPLDDEG----ALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLE---LETTNRAAAFYEKLG 81
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 132-193 3.90e-09

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 51.51  E-value: 3.90e-09
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15225009 132 LNDVVVAGFVLFFPNYSSflsKPGFYIEDIFVREPYRRKGFGSMLLTAVAKQAVKMGYGRVE 193
Cdd:cd04301   5 EDDGEIVGFASLSPDGSG---GDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLR 63
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 157-211 9.63e-09

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 52.30  E-value: 9.63e-09
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*
gi 15225009 157 YIEDIFVREPYRRKGFGSMLLTAVAKQAVKMGYGRVEWVVldwNVNAIKFYEQMG 211
Cdd:COG1246  54 ELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLLT---TSAAIHFYEKLG 105
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 155-211 5.23e-08

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 49.96  E-value: 5.23e-08
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 15225009   155 GFYIEDIFVREPYRRKGFGSMLLTAVAKQAVKMGYGRVEwVVLDWNVNAIKFYEQMG 211
Cdd:pfam13673  51 RGHISLLFVDPDYQGQGIGKALLEAVEDYAEKDGIKLSE-LTVNASPYAVPFYEKLG 106
FR47 pfam08445
FR47-like protein; The members of this family are similar to the C-terminal region of the D. ...
 151-220 3.28e-06

FR47-like protein; The members of this family are similar to the C-terminal region of the D. melanogaster hypothetical protein FR47. This protein has been found to consist of two N-acyltransferase-like domains swapped with the C-terminal strands.


Pssm-ID: 117022 [Multi-domain]  Cd Length: 86  Bit Score: 44.24  E-value: 3.28e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15225009   151 LSKPGFYIEDIFVREPYRRKGFGSMLLTAVAKQAVKMGYGRVEWVVLDwNVNAIKFYEQMGAQ-ILQEWRV 220
Cdd:pfam08445  17 LRLPGGELGALQTLPEHRRRGLGSRLVAALARGIAERGITPFAVVVAG-NTPSRRLYEKLGFRkIDETYWV 86
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 161-213 8.12e-06

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 44.60  E-value: 8.12e-06
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 15225009 161 IFVREPYRRKGFGSMLLTAVAKQAVK-MGYGRVEWVVLDWNVNAIKFYEQMGAQ 213
Cdd:COG1670  93 YWLAPAYWGKGYATEALRALLDYAFEeLGLHRVEAEVDPDNTASIRVLEKLGFR 146
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
158-211 9.67e-05

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 40.94  E-value: 9.67e-05
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 15225009 158 IEDIFVREPYRRKGFGSMLLTAVAKQAVKMGYGRvewVVLDWNVNAIKFYEQMG 211
Cdd:COG2153  61 IGRVAVLPEYRGQGLGRALMEAAIEEARERGARR---IVLSAQAHAVGFYEKLG 111
PRK09831 PRK09831
GNAT family N-acetyltransferase;
150-222 2.85e-04

GNAT family N-acetyltransferase;


Pssm-ID: 182099  Cd Length: 147  Bit Score: 39.94  E-value: 2.85e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15225009  150 FLSKPGFYIEDIFVREPYRRKGFGSMLLTAVAKQAVKMgygrvewvVLDWNVNAIKFYEQMGAQILQEWRV-CR 222
Cdd:PRK09831  67 FITCIEHYIDMLFVDPEYTRRGVASALLKPLIKSESEL--------TVDASITAKPFFERYGFQTVKQQRVeCR 132
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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