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Conserved domains on  [gi|22325463|ref|NP_178511|]
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Magnesium transporter CorA-like family protein [Arabidopsis thaliana]

Protein Classification

magnesium transporter CorA family protein( domain architecture ID 1983)

magnesium transporter CorA family protein belonging to the CorA metal ion transporter (MIT) superfamily, similar to CorA which mediates both influx and efflux of magnesium, and may also transport nickel and cobalt

CATH:  3.30.460.20|1.20.58.340
Gene Ontology:  GO:0046873|GO:0030001
PubMed:  9573171|17619822|23046658|16179994
SCOP:  4000404|3000358

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MIT_CorA-like super family cl00459
metal ion transporter CorA-like divalent cation transporter superfamily; This superfamily of ...
 195-385 7.76e-27

metal ion transporter CorA-like divalent cation transporter superfamily; This superfamily of essential membrane proteins is involved in transporting divalent cations (uptake or efflux) across membranes. They are found in most bacteria and archaea, and in some eukaryotes. It is a functionally diverse group which includes the Mg2+ transporters of Escherichia coli and Salmonella typhimurium CorAs (which can also transport Co2+, and Ni2+ ), the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, and the Zn2+ transporter Salmonella typhimurium ZntB, which mediates the efflux of Zn2+ (and Cd2+). It includes five Saccharomyces cerevisiae members: i) two plasma membrane proteins, the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, ii) two mitochondrial inner membrane Mg2+ transporters: Mfm1p/Lpe10p, and Mrs2p, and iii) and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. It also includes a family of Arabidopsis thaliana members (AtMGTs), some of which are localized to distinct tissues, and not all of which can transport Mg2+. Thermotoga maritima CorA and Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, Mrs2p, and Alr1p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


The actual alignment was detected with superfamily member cd12833:

Pssm-ID: 469776 [Multi-domain]  Cd Length: 290  Bit Score: 108.74  E-value: 7.76e-27
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 195 PTSVSNLVVHIIDTHVDRLQDVVTELEIELDGVELEMDRGGF--------AMKKKMLDDRRFpklhLNLQRllQVIAH-- 264
Cdd:cd12833 102 PKSPGDFLAALAERLTDRMEDVIDELEDRLDELEERVLEEEDeelrgelaELRRQAIALRRY----LAPQR--DALERla 175

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 265 GEQVfprvkekcstkPWFLAEDIHSLEELIGRLRRLKENVGFIANRVTAIQAGLDSWQAEQINRKLYYLSFLSIIFLPLS 344
Cdd:cd12833 176 REDL-----------PWLSDDDRLRLREAADRLTRYIEDLDAIRERAAVLQEELTNRRAEQMNRRMYVLSIVAAIFLPLG 244

                       170       180       190       200
                ....*....|....*....|....*....|....*....|.
gi 22325463 345 IITGVFGMNVGGVPwtGQDKPelrDGFrnvMYICLIMLVLV 385
Cdd:cd12833 245 FLTGLLGINVGGIP--GAENP---WAF---WIFCGLLVVLA 277
 
Name Accession Description Interval E-value
ZntB-like_1 cd12833
Salmonella typhimurium Zn2+ transporter ZntB-like subgroup; A bacterial subgroup belonging to ...
 195-385 7.76e-27

Salmonella typhimurium Zn2+ transporter ZntB-like subgroup; A bacterial subgroup belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subgroup includes the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, which occur in proteins belonging to this subfamily, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213367 [Multi-domain]  Cd Length: 290  Bit Score: 108.74  E-value: 7.76e-27
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 195 PTSVSNLVVHIIDTHVDRLQDVVTELEIELDGVELEMDRGGF--------AMKKKMLDDRRFpklhLNLQRllQVIAH-- 264
Cdd:cd12833 102 PKSPGDFLAALAERLTDRMEDVIDELEDRLDELEERVLEEEDeelrgelaELRRQAIALRRY----LAPQR--DALERla 175

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 265 GEQVfprvkekcstkPWFLAEDIHSLEELIGRLRRLKENVGFIANRVTAIQAGLDSWQAEQINRKLYYLSFLSIIFLPLS 344
Cdd:cd12833 176 REDL-----------PWLSDDDRLRLREAADRLTRYIEDLDAIRERAAVLQEELTNRRAEQMNRRMYVLSIVAAIFLPLG 244

                       170       180       190       200
                ....*....|....*....|....*....|....*....|.
gi 22325463 345 IITGVFGMNVGGVPwtGQDKPelrDGFrnvMYICLIMLVLV 385
Cdd:cd12833 245 FLTGLLGINVGGIP--GAENP---WAF---WIFCGLLVVLA 277
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 64-386 1.02e-26

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 108.56  E-value: 1.02e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463    64 GKEFCWYHVELPKgnqklSQSAQHLIDAL-CPPLKLQDILAlASNGPFCGHVDGALVFRVNSP-GPASSSNTFKIAARI- 140
Cdd:pfam01544   1 EQGFVWLDLDSPT-----EAELEWLARTLgLDPLALEDLLD-PEERPRVEDYDDYFLLVLPGVnLDDSEEADELVTVSIl 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463   141 -TENSVITVSLGRVPRLGfspmgqsllsevpsvieehvlEFLLKMnhseEADNPVPTSVSNLVVHIIDTHVDRLQDVVTE 219
Cdd:pfam01544  75 lGDNFLITVRHEPLPALD---------------------EVRKRL----TLLGGGPLDPGDLLYRLLDEIVDRFLELLEK 129

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463   220 LEIELDGVELEMDRGGfamKKKMLddRRFPKLHLNLQRLLQVIAHGEQVFPRVKEKCSTkPWFLAEDIHSLEELIGRLRR 299
Cdd:pfam01544 130 LEDELDELEDELEDET---NNELL--RELAALRRSLVYLRRSLLPQRDVLNRLLRREDD-PLLDDEQKEYLRDLLDRLER 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463   300 LKENVGFIANRVTAIQAGLDSWQAEQINRKLYYLSFLSIIFLPLSIITGVFGMNVGGVPWTgqdkpELRDGFRNVMyICL 379
Cdd:pfam01544 204 LLEDLDALRERLRSLQDEYSSLLSERTNRVMKVLTVVTAIFLPLTLITGIYGMNFGGLPEL-----EWSYGYPFVL-ILG 277


                  ....*..
gi 22325463   380 IMLVLVL 386
Cdd:pfam01544 278 LMILLAI 284
CorA COG0598
Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];
64-386 2.50e-19

Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];


Pssm-ID: 440363 [Multi-domain]  Cd Length: 305  Bit Score: 87.96  E-value: 2.50e-19
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463  64 GKEFCWYHVELPkgnqklSQSAQHLIDALC--PPLKLQDILAlASNGPFCGHVDGALVFRVNSPGPASSSNTFK---IAA 138
Cdd:COG0598  12 PEGFVWIDLDGP------TDEELEWLAEEFglHPLALEDALD-IEQRPKLEEYDDYLFIVLRTLNYNEEEEELEtgpLSI 84

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 139 RITENSVITVSLGRVPRLgfspmgqsllsevpsvieEHVLEFLlkmnhsEEADNPVPTSVSNLVVHIIDTHVDRLQDVVT 218
Cdd:COG0598  85 FLGPNFLITVRERPLDAF------------------DDVRERL------ERGPGLLPRGPDYLLYALLDAIVDRYFPVLE 140

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 219 ELEIELDGVELEMDRGG--------FAMKKKMLDDRRfpklHLN-LQRLLQVIAHGEQvfprvkekcstkPWFLAEDIHS 289
Cdd:COG0598 141 ELEDELDELEDELFESPdnellrrlYRLRRELVRLRR----ALApLREVLSRLLRGES------------PLISEEDREY 204

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 290 LEELIGRLRRLKENVGFIANRVTAIQAGLDSWQAEQINRKLYYLSFLSIIFLPLSIITGVFGMNVGGVPWTGQdkpelRD 369
Cdd:COG0598 205 LRDVYDHLERLLEMVDSLRERLSGLLDAYLSLISNRQNEIMKVLTIIAAIFLPPTFIAGIYGMNFEGMPELDW-----PY 279

                       330
                ....*....|....*..
gi 22325463 370 GFrnvMYICLIMLVLVL 386
Cdd:COG0598 280 GY---PIVLGLMLLIAV 293
zntB PRK09546
zinc transporter ZntB;
195-411 1.14e-13

zinc transporter ZntB;


Pssm-ID: 181941  Cd Length: 324  Bit Score: 71.57  E-value: 1.14e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463  195 PTSVSNLVVHIIDTHVDRLQDVVTELE---IELDGVELEMD---RGGFAM-KKKMLDDRRFpklhLNLQRllqviahgeQ 267
Cdd:PRK09546 137 PTDCGGWLVDVCDALTDHASEFIEELHdkiIDLEDNLLDQQippRGELALlRKQLIVMRRY----MAPQR---------D 203

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463  268 VFPRV-KEKCstkPWFLAEDIHSLEELIGRLRR-LKENVGFIAnRVTAIQAGLDSWQAEQINRKLYYLSFLSIIFLPLSI 345
Cdd:PRK09546 204 VFARLaSERL---PWMSDDDRRRMQDIADRLGRgLDDLDACIA-RTAVLADEIASVMAEAMNRRTYTMSLMAMVFLPTTF 279

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 22325463  346 ITGVFGMNVGGVPwtGQDKPElrdGFrnVMYiCLIMLVLVLCcfgfpalysriASWWgtrdMKRS-W 411
Cdd:PRK09546 280 LTGLFGVNLGGIP--GGGWPF---GF--SIF-CLLLVVLIGG-----------VAWW----LKRSkW 323
 
Name Accession Description Interval E-value
ZntB-like_1 cd12833
Salmonella typhimurium Zn2+ transporter ZntB-like subgroup; A bacterial subgroup belonging to ...
 195-385 7.76e-27

Salmonella typhimurium Zn2+ transporter ZntB-like subgroup; A bacterial subgroup belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subgroup includes the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, which occur in proteins belonging to this subfamily, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213367 [Multi-domain]  Cd Length: 290  Bit Score: 108.74  E-value: 7.76e-27
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 195 PTSVSNLVVHIIDTHVDRLQDVVTELEIELDGVELEMDRGGF--------AMKKKMLDDRRFpklhLNLQRllQVIAH-- 264
Cdd:cd12833 102 PKSPGDFLAALAERLTDRMEDVIDELEDRLDELEERVLEEEDeelrgelaELRRQAIALRRY----LAPQR--DALERla 175

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 265 GEQVfprvkekcstkPWFLAEDIHSLEELIGRLRRLKENVGFIANRVTAIQAGLDSWQAEQINRKLYYLSFLSIIFLPLS 344
Cdd:cd12833 176 REDL-----------PWLSDDDRLRLREAADRLTRYIEDLDAIRERAAVLQEELTNRRAEQMNRRMYVLSIVAAIFLPLG 244

                       170       180       190       200
                ....*....|....*....|....*....|....*....|.
gi 22325463 345 IITGVFGMNVGGVPwtGQDKPelrDGFrnvMYICLIMLVLV 385
Cdd:cd12833 245 FLTGLLGINVGGIP--GAENP---WAF---WIFCGLLVVLA 277
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 64-386 1.02e-26

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 108.56  E-value: 1.02e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463    64 GKEFCWYHVELPKgnqklSQSAQHLIDAL-CPPLKLQDILAlASNGPFCGHVDGALVFRVNSP-GPASSSNTFKIAARI- 140
Cdd:pfam01544   1 EQGFVWLDLDSPT-----EAELEWLARTLgLDPLALEDLLD-PEERPRVEDYDDYFLLVLPGVnLDDSEEADELVTVSIl 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463   141 -TENSVITVSLGRVPRLGfspmgqsllsevpsvieehvlEFLLKMnhseEADNPVPTSVSNLVVHIIDTHVDRLQDVVTE 219
Cdd:pfam01544  75 lGDNFLITVRHEPLPALD---------------------EVRKRL----TLLGGGPLDPGDLLYRLLDEIVDRFLELLEK 129

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463   220 LEIELDGVELEMDRGGfamKKKMLddRRFPKLHLNLQRLLQVIAHGEQVFPRVKEKCSTkPWFLAEDIHSLEELIGRLRR 299
Cdd:pfam01544 130 LEDELDELEDELEDET---NNELL--RELAALRRSLVYLRRSLLPQRDVLNRLLRREDD-PLLDDEQKEYLRDLLDRLER 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463   300 LKENVGFIANRVTAIQAGLDSWQAEQINRKLYYLSFLSIIFLPLSIITGVFGMNVGGVPWTgqdkpELRDGFRNVMyICL 379
Cdd:pfam01544 204 LLEDLDALRERLRSLQDEYSSLLSERTNRVMKVLTVVTAIFLPLTLITGIYGMNFGGLPEL-----EWSYGYPFVL-ILG 277


                  ....*..
gi 22325463   380 IMLVLVL 386
Cdd:pfam01544 278 LMILLAI 284
CorA COG0598
Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];
64-386 2.50e-19

Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];


Pssm-ID: 440363 [Multi-domain]  Cd Length: 305  Bit Score: 87.96  E-value: 2.50e-19
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463  64 GKEFCWYHVELPkgnqklSQSAQHLIDALC--PPLKLQDILAlASNGPFCGHVDGALVFRVNSPGPASSSNTFK---IAA 138
Cdd:COG0598  12 PEGFVWIDLDGP------TDEELEWLAEEFglHPLALEDALD-IEQRPKLEEYDDYLFIVLRTLNYNEEEEELEtgpLSI 84

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 139 RITENSVITVSLGRVPRLgfspmgqsllsevpsvieEHVLEFLlkmnhsEEADNPVPTSVSNLVVHIIDTHVDRLQDVVT 218
Cdd:COG0598  85 FLGPNFLITVRERPLDAF------------------DDVRERL------ERGPGLLPRGPDYLLYALLDAIVDRYFPVLE 140

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 219 ELEIELDGVELEMDRGG--------FAMKKKMLDDRRfpklHLN-LQRLLQVIAHGEQvfprvkekcstkPWFLAEDIHS 289
Cdd:COG0598 141 ELEDELDELEDELFESPdnellrrlYRLRRELVRLRR----ALApLREVLSRLLRGES------------PLISEEDREY 204

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 290 LEELIGRLRRLKENVGFIANRVTAIQAGLDSWQAEQINRKLYYLSFLSIIFLPLSIITGVFGMNVGGVPWTGQdkpelRD 369
Cdd:COG0598 205 LRDVYDHLERLLEMVDSLRERLSGLLDAYLSLISNRQNEIMKVLTIIAAIFLPPTFIAGIYGMNFEGMPELDW-----PY 279

                       330
                ....*....|....*..
gi 22325463 370 GFrnvMYICLIMLVLVL 386
Cdd:COG0598 280 GY---PIVLGLMLLIAV 293
ZntB_u1 cd12834
Uncharacterized bacterial subgroup of the Salmonella typhimurium Zn2+ transporter ZntB-like ...
 186-386 1.37e-18

Uncharacterized bacterial subgroup of the Salmonella typhimurium Zn2+ transporter ZntB-like subfamily; The MIT superfamily of essential membrane proteins is involved in transporting divalent cations (uptake or efflux) across membranes. The ZntB-like subfamily includes the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN which occur in proteins belonging to this subfamily, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213368 [Multi-domain]  Cd Length: 290  Bit Score: 85.72  E-value: 1.37e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 186 HSEEADNPVPTSVSnLVVHIIDTHVDRLQDVVTELEIELDGVE--LEMDRGGfAMKKKMLDDRRfpkLHLNLQRLLQVIA 263
Cdd:cd12834  94 QAVERGLRFRSPAD-LLEHLLREQADTLSRIVRELTDELDRIEdrLLSGRLR-RERRELGALRR---RLVRLHRLLAPEP 168

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 264 HgeqVFPRVKEkcSTKPWFLAEDIHSLEELIGRLRRLKENVGFIANRVTAIQAGLDSWQAEQINRKLYYLSFLSIIFLPL 343
Cdd:cd12834 169 A---ALFRLLR--RPPDWLPDADLETLRRLTERLDALDRDLVALQERARLLQEEIAARLAEQTNRSLYILSVVTALLLPP 243

                       170       180       190       200
                ....*....|....*....|....*....|....*....|....
gi 22325463 344 SIITGVFGMNVGGVPWTGQDkpelrDGFRNVMYICL-IMLVLVL 386
Cdd:cd12834 244 TLVTGLFGMNTGGLPLAEGP-----HGFWIAVALALlAAGLAYL 282
ZntB-like cd12824
Salmonella typhimurium Zn2+ transporter ZntB-like subfamily; A bacterial subfamily belonging ...
 190-407 7.64e-18

Salmonella typhimurium Zn2+ transporter ZntB-like subfamily; A bacterial subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subfamily includes the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, which occur in proteins belonging to this subfamily, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213358 [Multi-domain]  Cd Length: 290  Bit Score: 83.34  E-value: 7.64e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 190 ADNPVPTSVSNLVVHIIDTHVDRLQDVVTELEIELDGVELEMDRGGFAMKKKMLDDRRfpKLHLNLQRLLQVIahgEQVF 269
Cdd:cd12824  97 RDHSSLKSLADLLAQMISGMTKRLELVIFDLTETLDEVEDNLLDGGTAPPPYALIALQ--RRLFRIRRQIRPQ---QQAL 171

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 270 PRVKEkcSTKPWFLAEDIHSLEELIGRLRRLKENVGFIANRVTAIQAGLDSWQAEQINRKLYYLSFLSIIFLPLSIITGV 349
Cdd:cd12824 172 RPLIT--DPAELLVTSDRETLRRASNNVTRYLETLDFCRTRVALLQDEIKAQRDEKTTRSSLLLTVVATVFLPLTFITGL 249

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 22325463 350 FGMNVGGVPwtGQDKPElrdGFrnvMYICLIMLVLVLccfgfpalysrIASWWGTRDM 407
Cdd:cd12824 250 LGMNVGGIP--DVESPM---GF---WWVTGLSILVAL-----------LAWLLLRRLG 288
zntB PRK09546
zinc transporter ZntB;
195-411 1.14e-13

zinc transporter ZntB;


Pssm-ID: 181941  Cd Length: 324  Bit Score: 71.57  E-value: 1.14e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463  195 PTSVSNLVVHIIDTHVDRLQDVVTELE---IELDGVELEMD---RGGFAM-KKKMLDDRRFpklhLNLQRllqviahgeQ 267
Cdd:PRK09546 137 PTDCGGWLVDVCDALTDHASEFIEELHdkiIDLEDNLLDQQippRGELALlRKQLIVMRRY----MAPQR---------D 203

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463  268 VFPRV-KEKCstkPWFLAEDIHSLEELIGRLRR-LKENVGFIAnRVTAIQAGLDSWQAEQINRKLYYLSFLSIIFLPLSI 345
Cdd:PRK09546 204 VFARLaSERL---PWMSDDDRRRMQDIADRLGRgLDDLDACIA-RTAVLADEIASVMAEAMNRRTYTMSLMAMVFLPTTF 279

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 22325463  346 ITGVFGMNVGGVPwtGQDKPElrdGFrnVMYiCLIMLVLVLCcfgfpalysriASWWgtrdMKRS-W 411
Cdd:PRK09546 280 LTGLFGVNLGGIP--GGGWPF---GF--SIF-CLLLVVLIGG-----------VAWW----LKRSkW 323
TmCorA-like cd12822
Thermotoga maritima CorA-like family; This family belongs to the MIT superfamily of essential ...
 118-385 1.07e-12

Thermotoga maritima CorA-like family; This family belongs to the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of the Thermotoga maritima CorA_like family are found in all three kingdoms of life. It is a functionally diverse family, in addition to the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, it includes three Saccharomyces cerevisiae members: two plasma membrane proteins, the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213356 [Multi-domain]  Cd Length: 289  Bit Score: 68.36  E-value: 1.07e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 118 LVFRVNSPGPASS-SNTFKIAARITENSVITVSLGRvprlgfspmgqslLSEVPSVIEEhvlefllkmnhSEEADNPVPT 196
Cdd:cd12822  47 LVLRALRYDEEELeLETSELSIFLGKNFLVTVHEGP-------------LRLIDEVIER-----------LLSDPKLLPK 102

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 197 SVSNLVVHIIDTHVDRLQDVVTELEIELDGVELEM-DRGGFAMKKKMLDDRR----FPKLHLNLQRLLQVIAHGEQVFPR 271
Cdd:cd12822 103 GPDFLLYAILDAIVDSYFPILDELEDRLDELEDEIlEEPRREVLRELFALRRellrLRRILSPQREVLNRLLRRENELIS 182

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 272 VKekcsTKPWFlaEDIHS-LEELIGRLRRLKENVGfianrvTAIQAGLdSWQAEQINRKLYYLSFLSIIFLPLSIITGVF 350
Cdd:cd12822 183 EE----LRPYF--RDVYDhLERLAELLDSYRDLLS------SLLDAYL-SLQSNRTNEVMKVLTIITTIFLPLTFITGIY 249

                       250       260       270
                ....*....|....*....|....*....|....*
gi 22325463 351 GMNVGGVpwtgqdkPELRDgfRNVMYICLIMLVLV 385
Cdd:cd12822 250 GMNFEYM-------PELHW--PYGYFIVLGIMLLI 275
EcCorA_ZntB-like cd12821
Escherichia coli CorA-Salmonella typhimurium ZntB_like family; A family of the MIT superfamily ...
 69-397 1.85e-12

Escherichia coli CorA-Salmonella typhimurium ZntB_like family; A family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of this family are found in all three kingdoms of life. It is a functionally diverse family, including the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs (which can also transport Co2+, and Ni2+ ), and the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). It also includes two Saccharomyces cerevisiae members: the inner membrane Mg2+ transporters Mfm1p/Lpe10p, and Mrs2p, and a family of Arabidopsis thaliana members (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213355 [Multi-domain]  Cd Length: 285  Bit Score: 67.34  E-value: 1.85e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463  69 WYHVELPKgnqKLSQSAQHLIDALCPPLKLQDILALASNGPFCgHVDGALVFrVNSPGPASSSNTFKIAARITENSVITV 148
Cdd:cd12821   1 WYDIDEPD---DEIRRLLEELESSHLPNDLRDILLGVSFPRFE-EVSGSGIY-IQLRKPQKDADTASFHFLITRDRLITV 75

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 149 SLGRVPRLgfSPMGQSLLSEvpsvieehvlefllkmnhseeaDNPVPTSVSNLVVHIIDTHVDRLQDVVTELEIELDGVE 228
Cdd:cd12821  76 RLDELPAL--DELRQDLRSG----------------------QMKSAKSAAELLFVLLGAIIKALLTGIDQFEEKLEELE 131

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 229 LEMDRGgfaMKKKMLDDrrfpklHLNLQRLLQVIAHGEQVFPRVKEKCSTKP--WFLAEDIHSLEELIGRLRRLKENVGF 306
Cdd:cd12821 132 WDLLEG---NNAIKLDR------ILELRRELLRLTNLIEPQQEVLMALQEAFaeLLFSEDEEELRRTLDRIERLLQLIEE 202

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 307 IANRVTAIQAGLDSWQAEQINRKLYYLSFLSIIFLPLSIITGVFGMNVGGVPWTgqdkpELRDGFrnvMYICLIMLVLvl 386
Cdd:cd12821 203 YEQELDTLQDIEEVVSSHRGNEIMKTLTVVTTVFLPLTLIGGLWGMNFKNMPEL-----EWKYGY---LFVLGLIILS-- 272

                       330
                ....*....|.
gi 22325463 387 ccFGFPALYSR 397
Cdd:cd12821 273 --TAGLYLYLR 281
Alr1p-like cd12829
Saccharomyces cerevisiae Alr1p-like subfamily; This eukaryotic subfamily belongs to the ...
 175-389 2.22e-09

Saccharomyces cerevisiae Alr1p-like subfamily; This eukaryotic subfamily belongs to the Thermotoga maritima CorA (TmCorA)-family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subfamily includes three Saccharomyces cerevisiae members: two plasma membrane proteins, the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213363 [Multi-domain]  Cd Length: 305  Bit Score: 58.43  E-value: 2.22e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 175 EHVLEFLLKMNHSeeadnpvPTSVSNLVVH-IIDTHVDRLQDVVTELEIELDGVE----LEMDRGGFAMKKKMLDDRRFP 249
Cdd:cd12829  89 ERVRKRIRLLSDS-------HLLSSDWVLYaLLDSIVDSFFPLVDSIEKEVDSIDelvlLISSSDQSDLLRRIGDLRRRV 161

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 250 KlhlNLQRLLqviAHGEQVFPRVKeKCSTKPWFLAEDIhsleELIGRLRRLKENVGFIANRVTAI-------------QA 316
Cdd:cd12829 162 T---SLLRLL---FAKSDVLKQLL-KRLMKKTFVSPDS----EVAIYLGDVQDHILTLLQKLNHYedllsrshsnylaQL 230

                       170       180       190       200       210       220       230
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 22325463 317 GLDSWQA-EQINRKLYYLSFLSIIFLPLSIITGVFGMNVgGVPWTGQDKPELRDGFrnvmYICLIMLVLVLCCF 389
Cdd:cd12829 231 SLEMSQEsNGTDKAMLRLTVIATIFLPLQLITGLFGMNV-KVPGQDIESLYWFFGI----VIVAIMVLVAIVVV 299
MIT_CorA-like cd11744
metal ion transporter CorA-like divalent cation transporter superfamily; This superfamily of ...
 69-358 3.98e-08

metal ion transporter CorA-like divalent cation transporter superfamily; This superfamily of essential membrane proteins is involved in transporting divalent cations (uptake or efflux) across membranes. They are found in most bacteria and archaea, and in some eukaryotes. It is a functionally diverse group which includes the Mg2+ transporters of Escherichia coli and Salmonella typhimurium CorAs (which can also transport Co2+, and Ni2+ ), the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, and the Zn2+ transporter Salmonella typhimurium ZntB, which mediates the efflux of Zn2+ (and Cd2+). It includes five Saccharomyces cerevisiae members: i) two plasma membrane proteins, the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, ii) two mitochondrial inner membrane Mg2+ transporters: Mfm1p/Lpe10p, and Mrs2p, and iii) and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. It also includes a family of Arabidopsis thaliana members (AtMGTs), some of which are localized to distinct tissues, and not all of which can transport Mg2+. Thermotoga maritima CorA and Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, Mrs2p, and Alr1p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213354 [Multi-domain]  Cd Length: 286  Bit Score: 54.52  E-value: 3.98e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463  69 WYHVELPKGNQKLSQSAQHLIDalcpPLKLQDILALASNGPFCGHVDGALV--FRVNSPGPASSSNTFKIAARITENSVI 146
Cdd:cd11744   1 WIHCERLHDDDIRRVLEDNLHV----PRALEDILLAESRPSFHPLDDNFMIilRGFNMDENASPEDMLSVRILYFQGALI 76

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 147 TvsLGRVPRLGFSPMGQSLLSEvpsvieehvlefllkmnhseeadNPVPTSVSNLVVHIIDTHVDRLQDVVTELEIELDG 226
Cdd:cd11744  77 S--TREIPSRAFMEIRQRLREH-----------------------SKGPKSAASLLNQLIEGLNGKIDLYLETIEETLNE 131

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 227 VELEMDRGGF--------AMKKKMLDDRRFPKlhlNLQRLLQVIAHGEQVFPRvkekcstkpwflaEDIHSLEELIGRLR 298
Cdd:cd11744 132 LDVNDEEGHYdestynhiALQKALISIRRFIR---PQQYALRDLIRSESELVT-------------SRPHQYRFAHNNIT 195

                       250       260       270       280       290       300
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 299 RLKENVGFIANRVTAIQAGLDSWQAEQINRKLYYLSFLSIIFLPLSIITGVFGMNVGGVP 358
Cdd:cd11744 196 RINETIEFYLGEVALLQDEIKHNRDEKTNKNSKLFTLVATIFLPTTFLAGLYGMNFEGMP 255
EcCorA_ZntB-like_u1 cd12826
uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB ...
 174-391 1.85e-05

uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB family; A uncharacterized subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB (EcCorA-ZntB_like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. The EcCorA-ZntB_like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA-ZntB_like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213360  Cd Length: 281  Bit Score: 46.02  E-value: 1.85e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 174 EEHVLEFLLKMNHSEEADNP-VPTSVSNLVVHIIDTHVDRLQDvvteLEIELDGVELEMDRGG--------FAMKKKMLd 244
Cdd:cd12826  75 DGFVLKLLERIQEKKSWKGPsLERFLYDFLEYLIKDDLEYLEK----YEEKLEELEEEILDGKlknfneriLKIRKELL- 149

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 245 drrfpKLHLNLQRLL---QVIAHGEQVFprvkekcstkpwFLAEDIHSLEELIGRLRRLKENVGFIANRVTAIQaglDSW 321
Cdd:cd12826 150 -----RLNSYYEQLMdigEELEENENDL------------FDEEELRLFRLFSDRVERLHDNVQMLREYSLQIR---ELY 209

                       170       180       190       200       210       220       230
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 22325463 322 QAE---QINRKLYYLSFLSIIFLPLSIITGVFGMNVggvpwtgQDKPELRDGFRNVMYICLIMLVLVLCCFGF 391
Cdd:cd12826 210 QSQidiRQNRIMKILTVVTTIFLPLTLITGWYGMNF-------TNMPELHWKYGYPVVIILSILIVVIEIIYF 275
TmCorA-like_u2 cd12831
Uncharacterized bacterial subfamily of the Thermotoga maritima CorA-like family; This ...
 204-389 2.84e-05

Uncharacterized bacterial subfamily of the Thermotoga maritima CorA-like family; This subfamily belongs to the Thermotoga maritima CorA (TmCorA)-like family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of the TmCorA-like family are found in all three kingdoms of life. It is a functionally diverse family which includes the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, and three Saccharomyces cerevisiae proteins: two located in the plasma membrane: the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by a related protein, Saccharomyces cerevisiae Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213365 [Multi-domain]  Cd Length: 287  Bit Score: 45.62  E-value: 2.84e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 204 HIID---THVDRLQDVVTELEIEL--DGVELEMDRGgFAMKKKMLDDRR--FPKLHLnLQRLLQviahgEQVFPRVKEKc 276
Cdd:cd12831 112 KIVDdyfPIVYEIEDRLNEIEDNNvdSSNKKLMDEV-FDIRSDLLKLRRtiLPMRDL-LYRILN-----SERLEGDKEQ- 183

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 277 stKPWFlaEDIHSleeligRLRRLKENVgfIANR-VTA-IQAGLDSWQAEQINRKLYYLSFLSIIFLPLSIITGVFGMNV 354
Cdd:cd12831 184 --RAYF--QDIYD------HLLKLSEML--ESNReMTAdIRDNYESYNSNRMNSIMMTLTIISTIFMPLTFIAGIYGMNF 251

                       170       180       190
                ....*....|....*....|....*....|....*.
gi 22325463 355 ggvpwtgQDKPELRdgFRNVMYICL-IMLVLVLCCF 389
Cdd:cd12831 252 -------EYMPELK--WHYGYFVVLgIMLLIAIGMI 278
EcCorA-like_u1 cd12837
uncharacterized subgroup of the Escherichia coli Mg2+ transporter CorA_like subfamily; A ...
 191-353 5.39e-04

uncharacterized subgroup of the Escherichia coli Mg2+ transporter CorA_like subfamily; A uncharacterized subgroup of the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. The EcCorA_ZntB-like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA_ZntB-like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213371 [Multi-domain]  Cd Length: 298  Bit Score: 41.75  E-value: 5.39e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 191 DNPVPTSVSNLVVHIIDTHVDRLQDVVTELEIELDGVE---LEMDRGGFAMKKKMLDD--RRFPKLH----------LNL 255
Cdd:cd12837  96 QPSGCASGEDVLLGLLEAIVDRLADVLERVGADLDRLSrriFRSSAKRRRRASRDLRSvlRRIGRAGdllskireslLSL 175

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22325463 256 QRLLQVIAHGEQ--VFPRVKEKCSTkpwfLAEDIHSLEEligrlrrlkeNVGFIANRVTAIqagLDswqA-------EQI 326
Cdd:cd12837 176 GRLLSFLSANAAdwLSKELRARLKT----LQRDVASLSE----------HATFLSNKIQFL---LD---AtlgliniEQN 235

                       170       180
                ....*....|....*....|....*...
gi 22325463 327 NR-KLyyLSFLSIIFLPLSIITGVFGMN 353
Cdd:cd12837 236 NIiKI--FSVASVVFLPPTLIASIYGMN 261
EcCorA_ZntB-like_u2 cd12827
uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB ...
 336-389 5.75e-03

uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB family; A uncharacterized subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB (EcCorA-ZntB_like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes.The EcCorA-ZntB-like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA-ZntB-like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213361 [Multi-domain]  Cd Length: 289  Bit Score: 38.64  E-value: 5.75e-03
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 22325463 336 LSIIFLPLSIITGVFGMNVgGVPWtgQDKPelrDGFrnvMYICLIMLVLVLCCF 389
Cdd:cd12827 236 ITIVLTIPTIITGFYGMNV-PLPF--ADSP---YAF---WIILLISLLLSVIVA 280
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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