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Conserved domains on  [gi|15218162|ref|NP_175629|]
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Aquaporin-like superfamily protein [Arabidopsis thaliana]

Protein Classification

MIP/aquaporin family protein( domain architecture ID 239)

MIP (Major Intrinsic Protein)/aquaporin family protein similar to aquaporin that permits osmotically driven movement of water in both directions, and glycerol uptake facilitator protein that mediates glycerol diffusion across the cytoplasmic membrane via a pore-type mechanism

CATH:  1.20.1080.10
Gene Ontology:  GO:0055085|GO:0015267
PubMed:  8849412|11473620|11964478
SCOP:  3000508
TCDB:  1.A.8

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MIP super family cl00200
Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane ...
 29-122 1.59e-30

Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal cytosolic portion followed by six transmembrane helices, which might have arisen through gene duplication. On the basis of sequence similarity and functional characteristics, the superfamily can be subdivided into two major groups: water-selective channels called aquaporins (AQPs) and glycerol uptake facilitators (GlpFs). AQPs are found in all three kingdoms of life, while GlpFs have been characterized only within microorganisms.


The actual alignment was detected with superfamily member PLN00166:

Pssm-ID: 444743  Cd Length: 250  Bit Score: 109.26  E-value: 1.59e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218162   29 LEAVPTSNAIPIHSVAVRVGSTQRVVMEIIITFALVYTVYATAIDSNNGTLGTIAPLAIRLIVGANILAAGPFSGGPMNP 108
Cdd:PLN00166 119 LVFVTNGESVPTHGVAAGLGAIEGVVMEIVVTFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNP 198

                         90
                 ....*....|....
gi 15218162  109 GRSFGSSLAVGNFS 122
Cdd:PLN00166 199 ARSFGPAVVSGDFS 212
 
Name Accession Description Interval E-value
PLN00166 PLN00166
aquaporin TIP2; Provisional
 29-122 1.59e-30

aquaporin TIP2; Provisional


Pssm-ID: 165733  Cd Length: 250  Bit Score: 109.26  E-value: 1.59e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218162   29 LEAVPTSNAIPIHSVAVRVGSTQRVVMEIIITFALVYTVYATAIDSNNGTLGTIAPLAIRLIVGANILAAGPFSGGPMNP 108
Cdd:PLN00166 119 LVFVTNGESVPTHGVAAGLGAIEGVVMEIVVTFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNP 198

                         90
                 ....*....|....
gi 15218162  109 GRSFGSSLAVGNFS 122
Cdd:PLN00166 199 ARSFGPAVVSGDFS 212
MIP pfam00230
Major intrinsic protein; MIP (Major Intrinsic Protein) family proteins exhibit essentially two ...
 42-124 8.51e-23

Major intrinsic protein; MIP (Major Intrinsic Protein) family proteins exhibit essentially two distinct types of channel properties: (1) specific water transport by the aquaporins, and (2) small neutral solutes transport, such as glycerol by the glycerol facilitators.


Pssm-ID: 395174 [Multi-domain]  Cd Length: 223  Bit Score: 88.53  E-value: 8.51e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218162    42 SVAVRVGSTQRVVMEIIITFALVYTVYATAIDSNNGTLGTIAPLAIRLIVGANILAAGPFSGGPMNPGRSFGSSLAVGNF 121
Cdd:pfam00230 123 TLAPGVNAGQAFVVEIILTFQLVYCVFATTDDRRNGSLGTVAPLAIGFAVFLNHLAGGPYTGASMNPARSFGPAVVLWKW 202


                  ...
gi 15218162   122 SGH 124
Cdd:pfam00230 203 DDH 205
MIP cd00333
Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane ...
 47-124 6.86e-19

Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal cytosolic portion followed by six transmembrane helices, which might have arisen through gene duplication. On the basis of sequence similarity and functional characteristics, the superfamily can be subdivided into two major groups: water-selective channels called aquaporins (AQPs) and glycerol uptake facilitators (GlpFs). AQPs are found in all three kingdoms of life, while GlpFs have been characterized only within microorganisms.


Pssm-ID: 238204  Cd Length: 228  Bit Score: 78.45  E-value: 6.86e-19
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 15218162  47 VGSTQRVVMEIIITFALVYTVYATAIDSNNGTLGTIAPLAIRLIVGANILAAGPFSGGPMNPGRSFGSSLAVGNFSGH 124
Cdd:cd00333 127 VSNGNAFFVEFIGTFILVLVVFATTDDPNGPPPGGLAPLAIGLLVAAIGLAGGPITGASMNPARSLGPALFTGLARHW 204
MIP TIGR00861
MIP family channel proteins; 1.A.8 The Major Intrinsic Protein (MIP) FamilyThe MIP family is ...
 47-124 9.64e-14

MIP family channel proteins; 1.A.8 The Major Intrinsic Protein (MIP) FamilyThe MIP family is large and diverse, possessing over 100 members that all form transmembrane channels. These channel proteins function in water, smallcarbohydrate (e.g., glycerol), urea, NH3, CO2 and possibly ion transport by an energy independent mechanism. They are found ubiquitously in bacteria, archaeaand eukaryotes. The MIP family contains two major groups of channels: aquaporins and glycerol facilitators.The known aquaporins cluster loosely together as do the known glycerol facilitators. MIP family proteins are believed to form aqueous pores that selectively allow passive transport of their solute(s) across the membrane with minimal apparent recognition. Aquaporins selectively transport water (but not glycerol) while glycerol facilitators selectively transport glycerol but not water. Some aquaporins can transport NH3 and CO2. Glycerol facilitators function as solute nonspecific channels, and may transport glycerol, dihydroxyacetone, propanediol, urea and other small neutral molecules in physiologically importantprocesses. Some members of the family, including the yeast FPS protein (TC #1.A.8.5.1) and tobacco NtTIPA may transport both water and small solutes. [Transport and binding proteins, Unknown substrate]


Pssm-ID: 273306 [Multi-domain]  Cd Length: 216  Bit Score: 64.63  E-value: 9.64e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218162    47 VGSTQRVVMEIIITFALVYTVYATAIDSNNGTLGTIAPLAIRLIVGANILAAGPFSGGPMNPGRSFGSSLAVG--NFSGH 124
Cdd:TIGR00861 119 VSSGQAFFVEFIGTAILVLVIFATTDDRNRVPRGGFAPLAIGLLVFLIHLSMGPYTGTGMNPARSLGPALFAGlaGWGNH 198
GlpF COG0580
Glycerol uptake facilitator or related aquaporin (Major Intrinsic protein Family) ...
 12-123 1.65e-10

Glycerol uptake facilitator or related aquaporin (Major Intrinsic protein Family) [Carbohydrate transport and metabolism];


Pssm-ID: 440345 [Multi-domain]  Cd Length: 232  Bit Score: 55.90  E-value: 1.65e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218162  12 VASLTVsVWWFINKFTYLEAVPTSNAIPIHSVAVRVGSTQRVVMEIIITFALVYTVYATAidSNNGTLGTIAPLAIRLIV 91
Cdd:COG0580  94 LGAALV-YLIYGGHFDATEDGGAKLGVFATSPAIGYSLGAAFLTEIIGTFVLVFVILGIT--DKRAAPGGLAPLAIGLLV 170

                        90       100       110
                ....*....|....*....|....*....|..
gi 15218162  92 GANILAAGPFSGGPMNPGRSFGSSLAVGNFSG 123
Cdd:COG0580 171 AAIGLSLGPLTGYAINPARDLGPRLFHALGKG 202
 
Name Accession Description Interval E-value
PLN00166 PLN00166
aquaporin TIP2; Provisional
 29-122 1.59e-30

aquaporin TIP2; Provisional


Pssm-ID: 165733  Cd Length: 250  Bit Score: 109.26  E-value: 1.59e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218162   29 LEAVPTSNAIPIHSVAVRVGSTQRVVMEIIITFALVYTVYATAIDSNNGTLGTIAPLAIRLIVGANILAAGPFSGGPMNP 108
Cdd:PLN00166 119 LVFVTNGESVPTHGVAAGLGAIEGVVMEIVVTFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNP 198

                         90
                 ....*....|....
gi 15218162  109 GRSFGSSLAVGNFS 122
Cdd:PLN00166 199 ARSFGPAVVSGDFS 212
PLN00027 PLN00027
aquaporin TIP; Provisional
 37-124 3.25e-23

aquaporin TIP; Provisional


Pssm-ID: 177664  Cd Length: 252  Bit Score: 90.23  E-value: 3.25e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218162   37 AIPIHSVAVRVGSTQRVVMEIIITFALVYTVYATAIDSNNGTLGTIAPLAIRLIVGANILAAGPFSGGPMNPGRSFGSSL 116
Cdd:PLN00027 129 ETSAFSLSSGVGVWNAFVFEIVMTFGLVYTVYATAVDPKKGDLGIIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPAV 208


                 ....*...
gi 15218162  117 AVGNFSGH 124
Cdd:PLN00027 209 VSWTWTNH 216
MIP pfam00230
Major intrinsic protein; MIP (Major Intrinsic Protein) family proteins exhibit essentially two ...
 42-124 8.51e-23

Major intrinsic protein; MIP (Major Intrinsic Protein) family proteins exhibit essentially two distinct types of channel properties: (1) specific water transport by the aquaporins, and (2) small neutral solutes transport, such as glycerol by the glycerol facilitators.


Pssm-ID: 395174 [Multi-domain]  Cd Length: 223  Bit Score: 88.53  E-value: 8.51e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218162    42 SVAVRVGSTQRVVMEIIITFALVYTVYATAIDSNNGTLGTIAPLAIRLIVGANILAAGPFSGGPMNPGRSFGSSLAVGNF 121
Cdd:pfam00230 123 TLAPGVNAGQAFVVEIILTFQLVYCVFATTDDRRNGSLGTVAPLAIGFAVFLNHLAGGPYTGASMNPARSFGPAVVLWKW 202


                  ...
gi 15218162   122 SGH 124
Cdd:pfam00230 203 DDH 205
MIP cd00333
Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane ...
 47-124 6.86e-19

Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal cytosolic portion followed by six transmembrane helices, which might have arisen through gene duplication. On the basis of sequence similarity and functional characteristics, the superfamily can be subdivided into two major groups: water-selective channels called aquaporins (AQPs) and glycerol uptake facilitators (GlpFs). AQPs are found in all three kingdoms of life, while GlpFs have been characterized only within microorganisms.


Pssm-ID: 238204  Cd Length: 228  Bit Score: 78.45  E-value: 6.86e-19
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 15218162  47 VGSTQRVVMEIIITFALVYTVYATAIDSNNGTLGTIAPLAIRLIVGANILAAGPFSGGPMNPGRSFGSSLAVGNFSGH 124
Cdd:cd00333 127 VSNGNAFFVEFIGTFILVLVVFATTDDPNGPPPGGLAPLAIGLLVAAIGLAGGPITGASMNPARSLGPALFTGLARHW 204
PLN00167 PLN00167
aquaporin TIP5; Provisional
 38-121 3.27e-18

aquaporin TIP5; Provisional


Pssm-ID: 215085  Cd Length: 256  Bit Score: 77.25  E-value: 3.27e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218162   38 IPIHSVAVRVGSTQRVVMEIIITFALVYTVYAtAIDSNNGTLGTIAPLAIRLIVGANILAAGPFSGGPMNPGRSFGSSLA 117
Cdd:PLN00167 132 VPTYKIAEEMTGFGASVLEGVLTFGLVYTVYA-AGDPRRGLLGAIGPLAIGLVAGANVLAAGPFSGGSMNPACAFGSAVV 210


                 ....
gi 15218162  118 VGNF 121
Cdd:PLN00167 211 AGSF 214
MIP TIGR00861
MIP family channel proteins; 1.A.8 The Major Intrinsic Protein (MIP) FamilyThe MIP family is ...
 47-124 9.64e-14

MIP family channel proteins; 1.A.8 The Major Intrinsic Protein (MIP) FamilyThe MIP family is large and diverse, possessing over 100 members that all form transmembrane channels. These channel proteins function in water, smallcarbohydrate (e.g., glycerol), urea, NH3, CO2 and possibly ion transport by an energy independent mechanism. They are found ubiquitously in bacteria, archaeaand eukaryotes. The MIP family contains two major groups of channels: aquaporins and glycerol facilitators.The known aquaporins cluster loosely together as do the known glycerol facilitators. MIP family proteins are believed to form aqueous pores that selectively allow passive transport of their solute(s) across the membrane with minimal apparent recognition. Aquaporins selectively transport water (but not glycerol) while glycerol facilitators selectively transport glycerol but not water. Some aquaporins can transport NH3 and CO2. Glycerol facilitators function as solute nonspecific channels, and may transport glycerol, dihydroxyacetone, propanediol, urea and other small neutral molecules in physiologically importantprocesses. Some members of the family, including the yeast FPS protein (TC #1.A.8.5.1) and tobacco NtTIPA may transport both water and small solutes. [Transport and binding proteins, Unknown substrate]


Pssm-ID: 273306 [Multi-domain]  Cd Length: 216  Bit Score: 64.63  E-value: 9.64e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218162    47 VGSTQRVVMEIIITFALVYTVYATAIDSNNGTLGTIAPLAIRLIVGANILAAGPFSGGPMNPGRSFGSSLAVG--NFSGH 124
Cdd:TIGR00861 119 VSSGQAFFVEFIGTAILVLVIFATTDDRNRVPRGGFAPLAIGLLVFLIHLSMGPYTGTGMNPARSLGPALFAGlaGWGNH 198
PLN00026 PLN00026
aquaporin NIP; Provisional
 47-121 1.41e-10

aquaporin NIP; Provisional


Pssm-ID: 177663 [Multi-domain]  Cd Length: 298  Bit Score: 56.79  E-value: 1.41e-10
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15218162   47 VGSTQRVVMEIIITFALVYTVYATAIDSNngTLGTIAPLAIRLIVGANILAAGPFSGGPMNPGRSFGSSLAVGNF 121
Cdd:PLN00026 184 VSTGQAFALEFIITFNLLFVVTAVATDTR--AVGELAGIAVGATVMLNILVAGPSTGGSMNPVRTLGPAVAAGNY 256
GlpF COG0580
Glycerol uptake facilitator or related aquaporin (Major Intrinsic protein Family) ...
 12-123 1.65e-10

Glycerol uptake facilitator or related aquaporin (Major Intrinsic protein Family) [Carbohydrate transport and metabolism];


Pssm-ID: 440345 [Multi-domain]  Cd Length: 232  Bit Score: 55.90  E-value: 1.65e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218162  12 VASLTVsVWWFINKFTYLEAVPTSNAIPIHSVAVRVGSTQRVVMEIIITFALVYTVYATAidSNNGTLGTIAPLAIRLIV 91
Cdd:COG0580  94 LGAALV-YLIYGGHFDATEDGGAKLGVFATSPAIGYSLGAAFLTEIIGTFVLVFVILGIT--DKRAAPGGLAPLAIGLLV 170

                        90       100       110
                ....*....|....*....|....*....|..
gi 15218162  92 GANILAAGPFSGGPMNPGRSFGSSLAVGNFSG 123
Cdd:COG0580 171 AAIGLSLGPLTGYAINPARDLGPRLFHALGKG 202
PLN00184 PLN00184
aquaporin NIP1; Provisional
 51-123 1.93e-10

aquaporin NIP1; Provisional


Pssm-ID: 177778  Cd Length: 296  Bit Score: 56.57  E-value: 1.93e-10
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 15218162   51 QRVVMEIIITFALVYTVYATAIDsnNGTLGTIAPLAIRLIVGANILAAGPFSGGPMNPGRSFGSSLAVGNFSG 123
Cdd:PLN00184 179 QAFVMEFIVTFYLMFIISGVATD--NRAIGELAGLAIGSTVLLNVLIAAPVSSASMNPGRSLGPAMVYGCYKG 249
PLN00182 PLN00182
putative aquaporin NIP4; Provisional
 49-123 5.50e-09

putative aquaporin NIP4; Provisional


Pssm-ID: 165748  Cd Length: 283  Bit Score: 52.22  E-value: 5.50e-09
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15218162   49 STQRVVMEIIITFALVYTVYATAIDSNngTLGTIAPLAIRLIVGANILAAGPFSGGPMNPGRSFGSSLAVGNFSG 123
Cdd:PLN00182 158 SGQALVAEIIISFLLMFVISGVATDSR--AVGELAGIAVGMTIILNVFVAGPISGASMNPARSLGPAIVMGRYKG 230
PLN00183 PLN00183
putative aquaporin NIP7; Provisional
 55-121 1.26e-04

putative aquaporin NIP7; Provisional


Pssm-ID: 215092  Cd Length: 274  Bit Score: 39.84  E-value: 1.26e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15218162   55 MEIIITFALVYtvYATAIDSNNGTLGTIAPLAIRLIVGANILAAGPFSGGPMNPGRSFGSSLAVGNF 121
Cdd:PLN00183 166 VEFIATFIVMF--LAASLTSQPQSLGHLSGFVIGIAIGLAVLITGPVSGGSMNPARSLGPAIVSWDF 230
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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