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Conserved domains on  [gi|51092268|ref|NP_112326|]
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NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial precursor [Rattus norvegicus]

Protein Classification

complex I 24 kDa subunit family protein( domain architecture ID 11112961)

complex I 24 kDa subunit family protein is a thioredoxin-like ferredoxin subunit of an NADH dehydrogenase is involved in the reversible oxidation of molecular hydrogen using NADH or NADPH as electron donor or acceptor; such as bacterial NADH-quinone oxidoreductase subunit E (NuoE) and eukaryotic NADH-ubiquinone oxidoreductase 24 kDa subunit

EC:  7.1.1.-
Gene Ontology:  GO:0051537|GO:0016491
PubMed:  15916556|30277212

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
2Fe-2S_thioredx pfam01257
Thioredoxin-like [2Fe-2S] ferredoxin;
62-208 1.85e-94

Thioredoxin-like [2Fe-2S] ferredoxin;


:

Pssm-ID: 460139  Cd Length: 145  Bit Score: 273.58  E-value: 1.85e-94
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268    62 IEAIVRNYPegHRAAAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKYHIQVCTTTPCML 141
Cdd:pfam01257   1 IKEIIAKYP--RKRSALIPILHLAQEEYGYLPPEAIEYVAELLGIPPARVYEVATFYTMFSLKPVGKYHIQVCTGTPCHL 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 51092268   142 RDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEEIIDELR 208
Cdd:pfam01257  79 RGSDEILEALEKKLGIKPGETTPDGKFTLEEVECLGACDNAPVMQINDDYYGDLTPEKVDEILEELK 145
 
Name Accession Description Interval E-value
2Fe-2S_thioredx pfam01257
Thioredoxin-like [2Fe-2S] ferredoxin;
62-208 1.85e-94

Thioredoxin-like [2Fe-2S] ferredoxin;


Pssm-ID: 460139  Cd Length: 145  Bit Score: 273.58  E-value: 1.85e-94
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268    62 IEAIVRNYPegHRAAAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKYHIQVCTTTPCML 141
Cdd:pfam01257   1 IKEIIAKYP--RKRSALIPILHLAQEEYGYLPPEAIEYVAELLGIPPARVYEVATFYTMFSLKPVGKYHIQVCTGTPCHL 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 51092268   142 RDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEEIIDELR 208
Cdd:pfam01257  79 RGSDEILEALEKKLGIKPGETTPDGKFTLEEVECLGACDNAPVMQINDDYYGDLTPEKVDEILEELK 145
NuoE COG1905
NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) [Energy production and conversion]; ...
 50-209 2.47e-89

NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) [Energy production and conversion]; NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) is part of the Pathway/BioSystem: NADH dehydrogenase


Pssm-ID: 441509  Cd Length: 159  Bit Score: 260.85  E-value: 2.47e-89
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268  50 TPFDFTPENYERIEAIVRNYPegHRAAAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKY 129
Cdd:COG1905   2 SERELSEELLAEIDEIIAKYP--RKRSALIPILHLVQEEFGYLPEEAIEEIAEALGLPPAEVYGVATFYSMFRLKPVGKH 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268 130 HIQVCTTTPCMLRDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEEIIDELRA 209
Cdd:COG1905  80 VIRVCTGTACHLRGAEELLEALEEKLGIKPGETTADGKFTLEEVECLGACGLAPVMMVNDDVYGRLTPEKVDEILDELRA 159
PRK07539 PRK07539
NADH-quinone oxidoreductase subunit NuoE;
54-208 2.96e-89

NADH-quinone oxidoreductase subunit NuoE;


Pssm-ID: 181024  Cd Length: 154  Bit Score: 260.47  E-value: 2.96e-89
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268   54 FTPENYERIEAIVRNYPegHRAAAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKYHIQV 133
Cdd:PRK07539   2 LSAEELAAIEREIAKYP--RPRSAVIPALKIVQEQRGWVPDEAIEAVADYLGMPAIDVEEVATFYSMIFRQPVGRHVIQV 79

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 51092268  134 CTTTPCMLRDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEEIIDELR 208
Cdd:PRK07539  80 CTSTPCWLRGGEAILAALKKKLGIKPGETTADGRFTLLEVECLGACDNAPVVMINDDTYEDLTPEKIDELLDELK 154
nuoE_fam TIGR01958
NADH-quinone oxidoreductase, E subunit; This model describes the E chain of complexes that ...
 62-208 2.96e-78

NADH-quinone oxidoreductase, E subunit; This model describes the E chain of complexes that resemble NADH-quinone oxidoreductases. The electron acceptor is a quinone, ubiquinone, in mitochondria and most bacteria, including Escherichia coli, where the recommended gene symbol is nuoB. This model does not identify proteins from chloroplast and cyanobacteria. [Energy metabolism, Electron transport]


Pssm-ID: 131013  Cd Length: 148  Bit Score: 232.65  E-value: 2.96e-78
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268    62 IEAIVRNYPEGHRAAAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKYHIQVCTTTPCML 141
Cdd:TIGR01958   2 IEEIIAKYPDDQKRSAIMPALMIAQEQKGWVTPEAIAAVAEMLGIPPVWVYEVATFYSMFDTEPVGRYHLQVCTNVPCAL 81

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 51092268   142 RDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEEIIDELR 208
Cdd:TIGR01958  82 RGSEALLKYLENKLGIKPGETTPDGRFTLVEVECLGACGNAPVMMINDDYYEFLTPEKLDELLERYE 148
TRX_Fd_NuoE cd03064
TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E ...
 128-207 6.42e-46

TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E subfamily; Nuo, also called respiratory chain Complex 1, is the entry point for electrons into the respiratory chains of bacteria and the mitochondria of eukaryotes. It is a multisubunit complex with at least 14 core subunits. It catalyzes the electron transfer of NADH to quinone coupled with the transfer of protons across the membrane, providing the proton motive force required for energy-consuming processes. Electrons are transferred from NADH to quinone through a chain of iron-sulfur clusters in Nuo, including the [2Fe-2S] cluster present in NuoE core subunit, also called the 24 kD subunit of Complex 1. This subfamily also include formate dehydrogenases, NiFe hydrogenases and NAD-reducing hydrogenases, that contain a NuoE domain. A subset of these proteins contain both NuoE and NuoF in a single chain. NuoF, also called the 51 kD subunit of Complex 1, contains one [4Fe-4S] cluster and also binds the NADH substrate and FMN.


Pssm-ID: 239362 [Multi-domain]  Cd Length: 80  Bit Score: 148.05  E-value: 6.42e-46
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268 128 KYHIQVCTTTPCMLRDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEEIIDEL 207
Cdd:cd03064   1 KHVIRVCTGTACHLRGAEALLEALEKKLGIKPGETTPDGRFTLEEVECLGACDLAPVMMINDDVYGRLTPEKVDAILEAL 80
 
Name Accession Description Interval E-value
2Fe-2S_thioredx pfam01257
Thioredoxin-like [2Fe-2S] ferredoxin;
62-208 1.85e-94

Thioredoxin-like [2Fe-2S] ferredoxin;


Pssm-ID: 460139  Cd Length: 145  Bit Score: 273.58  E-value: 1.85e-94
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268    62 IEAIVRNYPegHRAAAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKYHIQVCTTTPCML 141
Cdd:pfam01257   1 IKEIIAKYP--RKRSALIPILHLAQEEYGYLPPEAIEYVAELLGIPPARVYEVATFYTMFSLKPVGKYHIQVCTGTPCHL 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 51092268   142 RDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEEIIDELR 208
Cdd:pfam01257  79 RGSDEILEALEKKLGIKPGETTPDGKFTLEEVECLGACDNAPVMQINDDYYGDLTPEKVDEILEELK 145
NuoE COG1905
NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) [Energy production and conversion]; ...
 50-209 2.47e-89

NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) [Energy production and conversion]; NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) is part of the Pathway/BioSystem: NADH dehydrogenase


Pssm-ID: 441509  Cd Length: 159  Bit Score: 260.85  E-value: 2.47e-89
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268  50 TPFDFTPENYERIEAIVRNYPegHRAAAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKY 129
Cdd:COG1905   2 SERELSEELLAEIDEIIAKYP--RKRSALIPILHLVQEEFGYLPEEAIEEIAEALGLPPAEVYGVATFYSMFRLKPVGKH 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268 130 HIQVCTTTPCMLRDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEEIIDELRA 209
Cdd:COG1905  80 VIRVCTGTACHLRGAEELLEALEEKLGIKPGETTADGKFTLEEVECLGACGLAPVMMVNDDVYGRLTPEKVDEILDELRA 159
PRK07539 PRK07539
NADH-quinone oxidoreductase subunit NuoE;
54-208 2.96e-89

NADH-quinone oxidoreductase subunit NuoE;


Pssm-ID: 181024  Cd Length: 154  Bit Score: 260.47  E-value: 2.96e-89
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268   54 FTPENYERIEAIVRNYPegHRAAAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKYHIQV 133
Cdd:PRK07539   2 LSAEELAAIEREIAKYP--RPRSAVIPALKIVQEQRGWVPDEAIEAVADYLGMPAIDVEEVATFYSMIFRQPVGRHVIQV 79

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 51092268  134 CTTTPCMLRDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEEIIDELR 208
Cdd:PRK07539  80 CTSTPCWLRGGEAILAALKKKLGIKPGETTADGRFTLLEVECLGACDNAPVVMINDDTYEDLTPEKIDELLDELK 154
nuoE_fam TIGR01958
NADH-quinone oxidoreductase, E subunit; This model describes the E chain of complexes that ...
 62-208 2.96e-78

NADH-quinone oxidoreductase, E subunit; This model describes the E chain of complexes that resemble NADH-quinone oxidoreductases. The electron acceptor is a quinone, ubiquinone, in mitochondria and most bacteria, including Escherichia coli, where the recommended gene symbol is nuoB. This model does not identify proteins from chloroplast and cyanobacteria. [Energy metabolism, Electron transport]


Pssm-ID: 131013  Cd Length: 148  Bit Score: 232.65  E-value: 2.96e-78
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268    62 IEAIVRNYPEGHRAAAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKYHIQVCTTTPCML 141
Cdd:TIGR01958   2 IEEIIAKYPDDQKRSAIMPALMIAQEQKGWVTPEAIAAVAEMLGIPPVWVYEVATFYSMFDTEPVGRYHLQVCTNVPCAL 81

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 51092268   142 RDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEEIIDELR 208
Cdd:TIGR01958  82 RGSEALLKYLENKLGIKPGETTPDGRFTLVEVECLGACGNAPVMMINDDYYEFLTPEKLDELLERYE 148
PRK12373 PRK12373
NADH-quinone oxidoreductase subunit E;
45-235 7.06e-78

NADH-quinone oxidoreductase subunit E;


Pssm-ID: 237082 [Multi-domain]  Cd Length: 400  Bit Score: 240.47  E-value: 7.06e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268   45 ENNPDTpFDFTPENYERIEAIVRNYPEGHRAAAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRK 124
Cdd:PRK12373   7 EDQPDS-FAFTPENAAWAEKQITKYPEGRQASAVIPLLMRAQEQEGWVTRAAIEKVADMLDMAYIRVLEVATFYTQFQLQ 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268  125 PVG-KYHIQVCTTTPCMLRDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEEI 203
Cdd:PRK12373  86 PVGtRAHIQVCGTTPCMLRGSEALMAVCKSKIHAHPHELNADGTLSWEEVECLGACVNAPMVQIGKDYYEDLTPERLEEI 165

                        170       180       190
                 ....*....|....*....|....*....|....
gi 51092268  204 IDELRAGKVP--KPGPRSGRFCCEPAGGLTSLTE 235
Cdd:PRK12373 166 IDAFAAGKGPvvKPGPQIGRYASEPAGGLTSLTE 199
TRX_Fd_NuoE cd03064
TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E ...
 128-207 6.42e-46

TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E subfamily; Nuo, also called respiratory chain Complex 1, is the entry point for electrons into the respiratory chains of bacteria and the mitochondria of eukaryotes. It is a multisubunit complex with at least 14 core subunits. It catalyzes the electron transfer of NADH to quinone coupled with the transfer of protons across the membrane, providing the proton motive force required for energy-consuming processes. Electrons are transferred from NADH to quinone through a chain of iron-sulfur clusters in Nuo, including the [2Fe-2S] cluster present in NuoE core subunit, also called the 24 kD subunit of Complex 1. This subfamily also include formate dehydrogenases, NiFe hydrogenases and NAD-reducing hydrogenases, that contain a NuoE domain. A subset of these proteins contain both NuoE and NuoF in a single chain. NuoF, also called the 51 kD subunit of Complex 1, contains one [4Fe-4S] cluster and also binds the NADH substrate and FMN.


Pssm-ID: 239362 [Multi-domain]  Cd Length: 80  Bit Score: 148.05  E-value: 6.42e-46
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268 128 KYHIQVCTTTPCMLRDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEEIIDEL 207
Cdd:cd03064   1 KHVIRVCTGTACHLRGAEALLEALEKKLGIKPGETTPDGRFTLEEVECLGACDLAPVMMINDDVYGRLTPEKVDAILEAL 80
PRK07571 PRK07571
bidirectional hydrogenase complex protein HoxE; Reviewed
 59-202 1.06e-32

bidirectional hydrogenase complex protein HoxE; Reviewed


Pssm-ID: 236053  Cd Length: 169  Bit Score: 117.16  E-value: 1.06e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268   59 YERIEAIVRNYpeGHRAAAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKYHIQVCTTTP 138
Cdd:PRK07571  21 FKVLEATMKRN--QYRQDALIEVLHKAQELFGYLERDLLLYVARQLKLPLSRVYGVATFYHLFSLKPSGEHTCVVCTGTA 98

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 51092268  139 CMLRDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEE 202
Cdd:PRK07571  99 CYVKGSAAILEDLENELGIKAGETTADGKLSLLTARCLGACGIAPAVVFDGKVAGKQTPESVLE 162
TRX_Fd_family cd02980
Thioredoxin (TRX)-like [2Fe-2S] Ferredoxin (Fd) family; composed of [2Fe-2S] Fds with a TRX ...
 129-207 2.55e-28

Thioredoxin (TRX)-like [2Fe-2S] Ferredoxin (Fd) family; composed of [2Fe-2S] Fds with a TRX fold (TRX-like Fds) and proteins containing domains similar to TRX-like Fd including formate dehydrogenases, NAD-reducing hydrogenases and the subunit E of NADH:ubiquinone oxidoreductase (NuoE). TRX-like Fds are soluble low-potential electron carriers containing a single [2Fe-2S] cluster. The exact role of TRX-like Fd is still unclear. It has been suggested that it may be involved in nitrogen fixation. Its homologous domains in large redox enzymes (such as Nuo and hydrogenases) function as electron carriers.


Pssm-ID: 239278 [Multi-domain]  Cd Length: 77  Bit Score: 102.71  E-value: 2.55e-28
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268 129 YHIQVCTTTPCMLRDSDSILETLQRKLGIKVGettpDKLFTLIEVECLGACVNAPMVQIN--DDYYEDLTPKDIEEIIDE 206
Cdd:cd02980   1 HHILVCTGTACGLRGAEELLEALEKELGIRGG----DGRVTVERVGCLGACGLAPVVVVYpdGVWYGRVTPEDVEEIVEE 76


                .
gi 51092268 207 L 207
Cdd:cd02980  77 L 77
PRK05988 PRK05988
formate dehydrogenase subunit gamma; Validated
 54-209 6.27e-24

formate dehydrogenase subunit gamma; Validated


Pssm-ID: 180339  Cd Length: 156  Bit Score: 93.87  E-value: 6.27e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268   54 FTPENYERIEAIVrnypEGHRA--AAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKYHI 131
Cdd:PRK05988   3 DEPWDAARIAAII----AEHKHleGALLPILHAIQDEFGYVPEDAVPVIAEALNLSRAEVHGVITFYHDFRTHPPGRHVL 78

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 51092268  132 QVCTTTPCMLRDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEEIIDELRA 209
Cdd:PRK05988  79 KLCRAEACQAMGGDALAAHAKARLGIDFHQTTADGAVTLEPVYCLGLCACSPAAMLDGEVHGRLDPQRLDALLAEARR 156
TRX_Fd_NuoE_FDH_gamma cd03081
TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E ...
 128-207 3.48e-14

TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E subfamily, NAD-dependent formate dehydrogenase (FDH) gamma subunit; composed of proteins similar to the gamma subunit of NAD-linked FDH of Ralstonia eutropha, a soluble enzyme that catalyzes the irreversible oxidation of formate to carbon dioxide accompanied by the reduction of NAD+ to NADH. FDH is a heteromeric enzyme composed of four nonidentical subunits (alpha, beta, gamma and delta). The FDH gamma subunit is closely related to NuoE, which is part of a multisubunit complex (Nuo) catalyzing the electron transfer of NADH to quinone coupled with the transfer of protons across the membrane. Electrons are transferred from NADH to quinone through a chain of iron-sulfur clusters in Nuo, including the [2Fe-2S] cluster present in NuoE. Similarly, the FDH gamma subunit is hypothesized to be involved in an electron transport chain involving other FDH subunits, upon the oxidation of formate.


Pssm-ID: 239379  Cd Length: 80  Bit Score: 65.87  E-value: 3.48e-14
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268 128 KYHIQVCTTTPCMLRDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEEIIDEL 207
Cdd:cd03081   1 RHVLKLCRAEACQAMGAEALAAHIKARLGIDFHETTADGSVTLEPVYCLGLCACSPAAMIDGEVHGRVDPEKFDALLAEL 80
COG3411 COG3411
2Fe-2S ferredoxin [Energy production and conversion]; 2Fe-2S ferredoxin is part of the Pathway ...
 128-212 6.16e-10

2Fe-2S ferredoxin [Energy production and conversion]; 2Fe-2S ferredoxin is part of the Pathway/BioSystem: 2Fe2S


Pssm-ID: 442637  Cd Length: 96  Bit Score: 54.90  E-value: 6.16e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268 128 KYHIQVCTTTpCMLRDSDSILETLQ---RKLGIKVGettpdklFTLIEVECLGACVNAPMVQINDD--YYEDLTPKDIEE 202
Cdd:COG3411   5 RHHVLVCTGS-CGAAGAEEVLDALKeelKERGLDGD-------VRVTKTGCLGRCEQGPVVVVYPEgvWYGNVTPEDVPE 76

                        90
                ....*....|.
gi 51092268 203 IIDE-LRAGKV 212
Cdd:COG3411  77 IVEEhLLGGRP 87
TRX_Fd_NuoE_hoxF cd03083
TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E ...
 128-207 2.92e-09

TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E subfamily, hoxF; composed of proteins similar to the NAD-reducing hydrogenase (hoxS) alpha subunit of Alcaligenes eutrophus H16. HoxS is a cytoplasmic hydrogenase catalyzing the oxidation of molecular hydrogen accompanied by the reduction of NAD. It is composed of four structural subunits encoded by the genes hoxF, hoxU, hoxY and hoxH. The hoxF protein (or alpha subunit) is a fusion protein containing an N-terminal NuoE-like domain and a C-terminal NuoF domain. NuoE and NuoF are components of Nuo, a multisubunit complex catalyzing the electron transfer of NADH to quinone coupled with the transfer of protons across the membrane. Electrons are transferred from NADH to quinone through a chain of iron-sulfur clusters in Nuo, including the [2Fe-2S] cluster in NuoE and the [4Fe-4S] cluster in NuoF. In addition, NuoF is also the NADH- and FMN-binding subunit. HoxF may be involved in the electron transport chain during the NAD-dependent oxidation of hydrogen through its NuoF domain. The NuoE-like domain of hoxF contains only one conserved cysteine in its putative active site, compared to four cysteines in NuoE, and may have lost the ability to bind [2Fe-2S] clusters.


Pssm-ID: 239381  Cd Length: 80  Bit Score: 52.41  E-value: 2.92e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 51092268 128 KYHIQVCTTTPCMLRDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDDYYEDLTPKDIEEIIDEL 207
Cdd:cd03083   1 KYRIYLSDSITDRMNGYKAVLDALCRELGIRFGEVDEDGMVGLFFTSCTGLCDQGPALLINNRVFTRLTPGRIDQIAELI 80
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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