NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1532527548|ref|NP_001354532|]
View 

rap guanine nucleotide exchange factor 5 isoform 5 [Homo sapiens]

Protein Classification

Ras-GEF domain-containing protein( domain architecture ID 10643694)

Ras guanine nucleotide exchange factor (Ras-GEF) domain-containing protein activates Ras-like small GTPases by mediating the replacement of GDP with GTP

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 68-200 2.55e-28

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


:

Pssm-ID: 214571  Cd Length: 127  Bit Score: 104.34  E-value: 2.55e-28
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1532527548   68 DRYVVVSGTPEKILEHLLndlhleEVQDKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKKYQ-GKEENSDVPRRKRK 146
Cdd:smart00229   1 DGGLIKGGTLEALIEHLT------EAFDKADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPEsWVEEKVNPRRVKNR 74

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....
gi 1532527548  147 VLHLVSQWIALYKDWLPEDEHSKMFLKTIYRNVldDVYEYPILEKELKEFQKIL 200
Cdd:smart00229  75 VLNILRTWVENYWEDFEDDPKLISFLLEFLELV--DDEKYPGLVTSLLNLLRRL 126
 
Name Accession Description Interval E-value
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 68-200 2.55e-28

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 104.34  E-value: 2.55e-28
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1532527548   68 DRYVVVSGTPEKILEHLLndlhleEVQDKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKKYQ-GKEENSDVPRRKRK 146
Cdd:smart00229   1 DGGLIKGGTLEALIEHLT------EAFDKADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPEsWVEEKVNPRRVKNR 74

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....
gi 1532527548  147 VLHLVSQWIALYKDWLPEDEHSKMFLKTIYRNVldDVYEYPILEKELKEFQKIL 200
Cdd:smart00229  75 VLNILRTWVENYWEDFEDDPKLISFLLEFLELV--DDEKYPGLVTSLLNLLRRL 126
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 76-199 1.18e-18

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


Pssm-ID: 100121  Cd Length: 122  Bit Score: 78.99  E-value: 1.18e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1532527548  76 TPEKILEHLLNDLhleevqDKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKKYQGKEENSDVP----RRKRKVLHLV 151
Cdd:cd06224     1 TLEALIEHLTSTF------DMPDPSFVSTFLLTYRSFTTPTELLEKLIERYEIAPPENLEYNDWDKkkskPIRLRVLNVL 74

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1532527548 152 SQWIALYKDWLPEDEHSKMFLKTIYRNVLDDVYEYPILEKELKEFQKI 199
Cdd:cd06224    75 RTWVENYPYDFFDDEELLELLEEFLNRLVQEGALLQELKKLLRKLLKL 122
RasGEF_N pfam00618
RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small ...
 71-173 1.09e-11

RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this motif/domain N-terminal to the RasGef (Cdc25-like) domain.


Pssm-ID: 459873  Cd Length: 104  Bit Score: 59.63  E-value: 1.09e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1532527548  71 VVVSGTPEKILEHLLNdlHLEEVQDKETETllddFLLTYTVFMTTDDLCQALLRHY----SAKKYQGKEENSDVPRR-KR 145
Cdd:pfam00618   1 QVKAGTLEKLVEYLTS--TRIMLDDSFLST----FLLTYRSFTTPAELLELLIERYnippPLDLSSDSYWISKKTLPiRI 74

                          90       100
                  ....*....|....*....|....*....
gi 1532527548 146 KVLHLVSQWIALY-KDWLPEDEHSKMFLK 173
Cdd:pfam00618  75 RVLSVLRHWVENYfSDFNDDPVLLSRLEK 103
 
Name Accession Description Interval E-value
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 68-200 2.55e-28

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 104.34  E-value: 2.55e-28
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1532527548   68 DRYVVVSGTPEKILEHLLndlhleEVQDKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKKYQ-GKEENSDVPRRKRK 146
Cdd:smart00229   1 DGGLIKGGTLEALIEHLT------EAFDKADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPEsWVEEKVNPRRVKNR 74

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....
gi 1532527548  147 VLHLVSQWIALYKDWLPEDEHSKMFLKTIYRNVldDVYEYPILEKELKEFQKIL 200
Cdd:smart00229  75 VLNILRTWVENYWEDFEDDPKLISFLLEFLELV--DDEKYPGLVTSLLNLLRRL 126
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 76-199 1.18e-18

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


Pssm-ID: 100121  Cd Length: 122  Bit Score: 78.99  E-value: 1.18e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1532527548  76 TPEKILEHLLNDLhleevqDKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKKYQGKEENSDVP----RRKRKVLHLV 151
Cdd:cd06224     1 TLEALIEHLTSTF------DMPDPSFVSTFLLTYRSFTTPTELLEKLIERYEIAPPENLEYNDWDKkkskPIRLRVLNVL 74

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1532527548 152 SQWIALYKDWLPEDEHSKMFLKTIYRNVLDDVYEYPILEKELKEFQKI 199
Cdd:cd06224    75 RTWVENYPYDFFDDEELLELLEEFLNRLVQEGALLQELKKLLRKLLKL 122
RasGEF_N pfam00618
RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small ...
 71-173 1.09e-11

RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this motif/domain N-terminal to the RasGef (Cdc25-like) domain.


Pssm-ID: 459873  Cd Length: 104  Bit Score: 59.63  E-value: 1.09e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1532527548  71 VVVSGTPEKILEHLLNdlHLEEVQDKETETllddFLLTYTVFMTTDDLCQALLRHY----SAKKYQGKEENSDVPRR-KR 145
Cdd:pfam00618   1 QVKAGTLEKLVEYLTS--TRIMLDDSFLST----FLLTYRSFTTPAELLELLIERYnippPLDLSSDSYWISKKTLPiRI 74

                          90       100
                  ....*....|....*....|....*....
gi 1532527548 146 KVLHLVSQWIALY-KDWLPEDEHSKMFLK 173
Cdd:pfam00618  75 RVLSVLRHWVENYfSDFNDDPVLLSRLEK 103
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH