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Conserved domains on  [gi|1063723753|ref|NP_001329420|]
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Acyl-CoA N-acyltransferases (NAT) superfamily protein [Arabidopsis thaliana]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10136841)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.1.-
Gene Ontology:  GO:0008080
PubMed:  10940244|9175471|15581578
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 149-192 9.46e-06

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


:

Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 42.26  E-value: 9.46e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 1063723753 149 NGQLVGFGRAYSDYGL--TASIHDLMVLPSLQRMGIGKLIVNRIVR 192
Cdd:cd04301     7 DGEIVGFASLSPDGSGgdTAYIGDLAVLPEYRGKGIGSALLEAAEE 52
ArgA super family cl43364
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 149-268 1.47e-05

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


The actual alignment was detected with superfamily member COG1246:

Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 43.44  E-value: 1.47e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063723753 149 NGQLVGFGRAYSDYGLTASIHDLMVLPSLQRMGIGKLIVNRIVrsdgifhyDFAllktlklllkvRSSFIKNCYnrLLTS 228
Cdd:COG1246    36 DGEIVGCAALHPLDEDLAELRSLAVHPDYRGRGIGRRLLEALL--------AEA-----------RELGLKRLF--LLTT 94

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1063723753 229 RdiydiaalcfeDERPFFKACGF---------GDDRMGSTTMMFTKSLE 268
Cdd:COG1246    95 S-----------AAIHFYEKLGFeeidkedlpYAKVWQRDSVVMEKDLE 132
 
Name Accession Description Interval E-value
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 149-192 9.46e-06

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 42.26  E-value: 9.46e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 1063723753 149 NGQLVGFGRAYSDYGL--TASIHDLMVLPSLQRMGIGKLIVNRIVR 192
Cdd:cd04301     7 DGEIVGFASLSPDGSGgdTAYIGDLAVLPEYRGKGIGSALLEAAEE 52
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 149-192 9.54e-06

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 43.66  E-value: 9.54e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 1063723753 149 NGQLVGFGRAYSDYG--LTASIHDLMVLPSLQRMGIGKLIVNRIVR 192
Cdd:pfam00583  41 DGELVGFASLSIIDDepPVGEIEGLAVAPEYRGKGIGTALLQALLE 86
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 149-268 1.47e-05

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 43.44  E-value: 1.47e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063723753 149 NGQLVGFGRAYSDYGLTASIHDLMVLPSLQRMGIGKLIVNRIVrsdgifhyDFAllktlklllkvRSSFIKNCYnrLLTS 228
Cdd:COG1246    36 DGEIVGCAALHPLDEDLAELRSLAVHPDYRGRGIGRRLLEALL--------AEA-----------RELGLKRLF--LLTT 94

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1063723753 229 RdiydiaalcfeDERPFFKACGF---------GDDRMGSTTMMFTKSLE 268
Cdd:COG1246    95 S-----------AAIHFYEKLGFeeidkedlpYAKVWQRDSVVMEKDLE 132
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
149-192 4.29e-04

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 39.68  E-value: 4.29e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 1063723753 149 NGQLVGFGRAY----SDYGLTASIHDLMVLPSLQRMGIGKLIVNRIVR 192
Cdd:COG3153    47 DGEIVGHVALSpvdiDGEGPALLLGPLAVDPEYRGQGIGRALMRAALE 94
 
Name Accession Description Interval E-value
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 149-192 9.46e-06

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 42.26  E-value: 9.46e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 1063723753 149 NGQLVGFGRAYSDYGL--TASIHDLMVLPSLQRMGIGKLIVNRIVR 192
Cdd:cd04301     7 DGEIVGFASLSPDGSGgdTAYIGDLAVLPEYRGKGIGSALLEAAEE 52
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 149-192 9.54e-06

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 43.66  E-value: 9.54e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 1063723753 149 NGQLVGFGRAYSDYG--LTASIHDLMVLPSLQRMGIGKLIVNRIVR 192
Cdd:pfam00583  41 DGELVGFASLSIIDDepPVGEIEGLAVAPEYRGKGIGTALLQALLE 86
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 149-268 1.47e-05

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 43.44  E-value: 1.47e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063723753 149 NGQLVGFGRAYSDYGLTASIHDLMVLPSLQRMGIGKLIVNRIVrsdgifhyDFAllktlklllkvRSSFIKNCYnrLLTS 228
Cdd:COG1246    36 DGEIVGCAALHPLDEDLAELRSLAVHPDYRGRGIGRRLLEALL--------AEA-----------RELGLKRLF--LLTT 94

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1063723753 229 RdiydiaalcfeDERPFFKACGF---------GDDRMGSTTMMFTKSLE 268
Cdd:COG1246    95 S-----------AAIHFYEKLGFeeidkedlpYAKVWQRDSVVMEKDLE 132
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 149-190 3.70e-04

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 38.59  E-value: 3.70e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|...
gi 1063723753 149 NGQLVGFGRAYSDYGLTASIH-DLMVLPSLQRMGIGKLIVNRI 190
Cdd:pfam13508  11 DGKIVGFAALLPLDDEGALAElRLAVHPEYRGQGIGRALLEAA 53
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
149-192 4.29e-04

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 39.68  E-value: 4.29e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 1063723753 149 NGQLVGFGRAY----SDYGLTASIHDLMVLPSLQRMGIGKLIVNRIVR 192
Cdd:COG3153    47 DGEIVGHVALSpvdiDGEGPALLLGPLAVDPEYRGQGIGRALMRAALE 94
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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