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Conserved domains on  [gi|1063693864|ref|NP_001323068|]
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triacylglycerol lipase-like 1 [Arabidopsis thaliana]

Protein Classification

alpha/beta hydrolase family protein( domain architecture ID 229394)

alpha/beta hydrolase family protein may catalyze the hydrolysis of substrates with different chemical composition or physicochemical properties using a nucleophile-His-acid catalytic triad

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Abhydrolase super family cl21494
alpha/beta hydrolases; A functionally diverse superfamily containing proteases, lipases, ...
 31-504 0e+00

alpha/beta hydrolases; A functionally diverse superfamily containing proteases, lipases, peroxidases, esterases, epoxide hydrolases and dehalogenases. The catalytic apparatus typically involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine, and often the mechanism involves a nucleophilic attack on a carbonyl carbon atom.


The actual alignment was detected with superfamily member PLN02162:

Pssm-ID: 473884  Cd Length: 475  Bit Score: 924.06  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864  31 MKFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFLQKVLMLLSKPFAFIGQKLT 110
Cdd:PLN02162    1 MKFCNSYFLVDPTKASFLDLLLLLFSFNLTSARFIDSPPDTLKGFRRSFASRWILALAIFLQKVLMLLSKPFAFIGQKLT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 111 YWLNLLTANGGFFNLILNLMSGKLVKPDKSSATYTSFIGCSDRRIELDEKINVGSIEYKSMLSIMASKISYESKPYITSV 190
Cdd:PLN02162   81 YWLNLLTANGGFFNLILNLLSGKLVKPDKSSATYTSFIGCSDRRIELDEKIDVGSIEYKSMLSIMASKISYESKPFINSV 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 191 VKNTWKMDLVGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRAL 270
Cdd:PLN02162  161 VKNTWKMDLVGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYELKNVGKVHAGFSRAL 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 271 GLQKDG-WPKENISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPAILAIHGEDELLDKLEGIYTFGQP 349
Cdd:PLN02162  241 GLQKDGgWPKENISLLHQYAYYTIRQMLRDKLARNKNLKYILTGHSLGGALAALFPAILAIHGEDELLDKLEGIYTFGQP 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 350 RVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFDDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQ 429
Cdd:PLN02162  321 RVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFDDKLLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQ 400

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1063693864 430 LLTGLWEFIRSFILQFWKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRPPPTTTPEDKLALIA 504
Cdd:PLN02162  401 LLTGLWEFIRSFILQFWKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRPPPSTTPEDKLALIA 475
 
Name Accession Description Interval E-value
PLN02162 PLN02162
triacylglycerol lipase
 31-504 0e+00

triacylglycerol lipase


Pssm-ID: 177821  Cd Length: 475  Bit Score: 924.06  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864  31 MKFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFLQKVLMLLSKPFAFIGQKLT 110
Cdd:PLN02162    1 MKFCNSYFLVDPTKASFLDLLLLLFSFNLTSARFIDSPPDTLKGFRRSFASRWILALAIFLQKVLMLLSKPFAFIGQKLT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 111 YWLNLLTANGGFFNLILNLMSGKLVKPDKSSATYTSFIGCSDRRIELDEKINVGSIEYKSMLSIMASKISYESKPYITSV 190
Cdd:PLN02162   81 YWLNLLTANGGFFNLILNLLSGKLVKPDKSSATYTSFIGCSDRRIELDEKIDVGSIEYKSMLSIMASKISYESKPFINSV 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 191 VKNTWKMDLVGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRAL 270
Cdd:PLN02162  161 VKNTWKMDLVGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYELKNVGKVHAGFSRAL 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 271 GLQKDG-WPKENISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPAILAIHGEDELLDKLEGIYTFGQP 349
Cdd:PLN02162  241 GLQKDGgWPKENISLLHQYAYYTIRQMLRDKLARNKNLKYILTGHSLGGALAALFPAILAIHGEDELLDKLEGIYTFGQP 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 350 RVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFDDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQ 429
Cdd:PLN02162  321 RVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFDDKLLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQ 400

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1063693864 430 LLTGLWEFIRSFILQFWKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRPPPTTTPEDKLALIA 504
Cdd:PLN02162  401 LLTGLWEFIRSFILQFWKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRPPPSTTPEDKLALIA 475
Lipase_3 cd00519
Lipase (class 3). Lipases are esterases that can hydrolyze long-chain acyl-triglycerides into ...
 167-404 2.61e-49

Lipase (class 3). Lipases are esterases that can hydrolyze long-chain acyl-triglycerides into di- and monoglycerides, glycerol, and free fatty acids at a water/lipid interface. A typical feature of lipases is "interfacial activation," the process of becoming active at the lipid/water interface, although several examples of lipases have been identified that do not undergo interfacial activation . The active site of a lipase contains a catalytic triad consisting of Ser - His - Asp/Glu, but unlike most serine proteases, the active site is buried inside the structure. A "lid" or "flap" covers the active site, making it inaccessible to solvent and substrates. The lid opens during the process of interfacial activation, allowing the lipid substrate access to the active site.


Pssm-ID: 238287 [Multi-domain]  Cd Length: 229  Bit Score: 169.19  E-value: 2.61e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 167 EYKSMLSIMASKISYESKPYITS--VVKNTWKMDLVGNYDFYNAFQESKLTQAFVFKTSSTNpdLIVVSFRGTEPFEaaD 244
Cdd:cd00519     2 YEKLKYYAKLAAAAYCVDANILAkaVVFADIALLNVFSPDKLLKTDKQYDTQGYVAVDHDRK--TIVIAFRGTVSLA--D 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 245 WCTDLDLSWYEMK----NVGKVHAGFSRALGLQKDGWPKENISLLHQYAYYTIrqmlrdklgrnknlkyILTGHSLGGAL 320
Cdd:cd00519    78 WLTDLDFSPVPLDpplcSGGKVHSGFYSAYKSLYNQVLPELKSALKQYPDYKI----------------IVTGHSLGGAL 141

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 321 AALFPAILAIHGEDELLdkleGIYTFGQPRVGDEDFGEFMKGVVKKHgieyERFVYNNDVVPRVPFDDKYL-FSYKHYGP 399
Cdd:cd00519   142 ASLLALDLRLRGPGSDV----TVYTFGQPRVGNAAFAEYLESTKGRV----YRVVHGNDIVPRLPPGSLTPpEGYTHVGT 213


                  ....*
gi 1063693864 400 CNSFN 404
Cdd:cd00519   214 EVWID 218
Lipase_3 pfam01764
Lipase (class 3);
231-388 9.50e-36

Lipase (class 3);


Pssm-ID: 396362 [Multi-domain]  Cd Length: 139  Bit Score: 129.69  E-value: 9.50e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 231 VVSFRGTEpfEAADWCTDLDLSWYEMKNV----GKVHAGFSRALGLqkdgwpkenisllhqyAYYTIRQMLRDKLGRNKN 306
Cdd:pfam01764   1 VVAFRGTN--SILDWLTDFDFSLTPFKDFflggGKVHSGFLSAYTS----------------VREQVLAELKRLLEKYPD 62

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 307 LKYILTGHSLGGALAALFPAILAIHGedELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHgieYERFVYNNDVVPRVPF 386
Cdd:pfam01764  63 YSIVVTGHSLGGALASLAALDLVENG--LRLSSRVTVVTFGQPRVGNLEFAKLHDSQGPKF---SYRVVHQRDIVPRLPP 137


                  ..
gi 1063693864 387 DD 388
Cdd:pfam01764 138 IV 139
Lip2 COG3675
Predicted lipase [Lipid transport and metabolism];
226-420 2.15e-33

Predicted lipase [Lipid transport and metabolism];


Pssm-ID: 442891 [Multi-domain]  Cd Length: 266  Bit Score: 127.56  E-value: 2.15e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 226 NPDLIVVSFRGTEpfEAADWCTDLDL---SWYEMKNVGKVHAGFSRAlglqkdgwpkenisllhqyaYYTIRQMLRDKLG 302
Cdd:COG3675    25 SDDEVIVAFRGTE--SLTDWLTNLNAaqvPYPFAKTGGKVHRGFYRA--------------------LQSLRELLEDALR 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 303 RNKNLKYI-LTGHSLGGALAALFpailAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHgieYeRFVYNNDVV 381
Cdd:COG3675    83 PLSPGKRLyVTGHSLGGALATLA----AADLERNYIFPVRGLYTFGQPRVGDRSFAKYYNLHVPNS---Y-RIVNNNDIV 154

                         170       180       190
                  ....*....|....*....|....*....|....*....
gi 1063693864 382 PRVPFddkYLFSYKHYGPCNSFNSLyKGKVREDAPNANY 420
Cdd:COG3675   155 PLLPP---VWMGYDHVGKLLWLDSL-RKDMLTDHSMDNY 189
 
Name Accession Description Interval E-value
PLN02162 PLN02162
triacylglycerol lipase
 31-504 0e+00

triacylglycerol lipase


Pssm-ID: 177821  Cd Length: 475  Bit Score: 924.06  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864  31 MKFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFLQKVLMLLSKPFAFIGQKLT 110
Cdd:PLN02162    1 MKFCNSYFLVDPTKASFLDLLLLLFSFNLTSARFIDSPPDTLKGFRRSFASRWILALAIFLQKVLMLLSKPFAFIGQKLT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 111 YWLNLLTANGGFFNLILNLMSGKLVKPDKSSATYTSFIGCSDRRIELDEKINVGSIEYKSMLSIMASKISYESKPYITSV 190
Cdd:PLN02162   81 YWLNLLTANGGFFNLILNLLSGKLVKPDKSSATYTSFIGCSDRRIELDEKIDVGSIEYKSMLSIMASKISYESKPFINSV 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 191 VKNTWKMDLVGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRAL 270
Cdd:PLN02162  161 VKNTWKMDLVGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYELKNVGKVHAGFSRAL 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 271 GLQKDG-WPKENISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPAILAIHGEDELLDKLEGIYTFGQP 349
Cdd:PLN02162  241 GLQKDGgWPKENISLLHQYAYYTIRQMLRDKLARNKNLKYILTGHSLGGALAALFPAILAIHGEDELLDKLEGIYTFGQP 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 350 RVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFDDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQ 429
Cdd:PLN02162  321 RVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFDDKLLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQ 400

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1063693864 430 LLTGLWEFIRSFILQFWKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRPPPTTTPEDKLALIA 504
Cdd:PLN02162  401 LLTGLWEFIRSFILQFWKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRPPPSTTPEDKLALIA 475
PLN00413 PLN00413
triacylglycerol lipase
 36-500 0e+00

triacylglycerol lipase


Pssm-ID: 165792  Cd Length: 479  Bit Score: 563.87  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864  36 SYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFLQKVLMLLSKPFAFIGQKLTYWLNL 115
Cdd:PLN00413    6 NYFVLDPREATVSDLIHLLFSSDLEDRKFIDSSEENIEDDLCEFRGRWIIFVSIVIQKLIIIFKKPLSFLGFALACWLNL 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 116 LTANGGFFNLILNLMSGKLVKPDKSSATYTSFIGCSDRRIE--LDEKINVGSIEYKSMLSIMASKISYESKPYITSVVKN 193
Cdd:PLN00413   86 LSSNGGFFKIFLNLFKGNFIWPEKASATFASINGNLDQKVElgLGPKIEIGDERYKALLSIMASKLAYENEHFIRSVLHD 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 194 TWKMDLVGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRALGLQ 273
Cdd:PLN00413  166 HWKMDLLGFYSCPNDFDKQRSTEVIVIKDTKDDPNLIIVSFRGTDPFDADDWCTDLDLSWHEVKNVGKIHGGFMKALGLP 245

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 274 KDGWPKE-----NISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPAILAIHGEDELLDKLEGIYTFGQ 348
Cdd:PLN00413  246 KEGWPEEinldeTQNATSLLAYYTILRHLKEIFDQNPTSKFILSGHSLGGALAILFTAVLIMHDEEEMLERLEGVYTFGQ 325

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 349 PRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFDDKYLFsYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIP 428
Cdd:PLN00413  326 PRVGDEDFGIFMKDKLKEFDVKYERYVYCNDMVPRLPFDDKTLM-FKHFGACLYCDSFYKGKVEEEEPNKNYFNIFWVIP 404

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1063693864 429 QLLTGLWEFIRSFILQFWKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRPPPTTT--PEDKL 500
Cdd:PLN00413  405 KIINALWELIRSFIIPCWKGGEFREGWFLRCFRLVALLIPGLPAHFPNEYINVALLGNFPPQVPDSQdlPDDDL 478
PLN02934 PLN02934
triacylglycerol lipase
 62-485 6.05e-141

triacylglycerol lipase


Pssm-ID: 215504  Cd Length: 515  Bit Score: 415.34  E-value: 6.05e-141
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864  62 ARFIDSPPDTLKGfRRSFASRWILALAIFLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVKPDKSS 141
Cdd:PLN02934   38 AKFLESSDERVPG-EEAVDHRWVILVSIIIRKIIALFGTPMEYTGFVVDFFLNLFSQNGGFLGLLLNLLQGKVVIPQRGS 116

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 142 ATYTSFIGCSDRRIEL----------DEKINVGSIEYKSM--------LSIMASKISYESKPYITSVVKNTWKMDLVGNY 203
Cdd:PLN02934  117 ETFISTIGHLDGRIDLyktpnlveqlDDSVSNHNSKIKGElgnralmdLCIMASKLAYENAKVVENVVDHHWKMHFVAFY 196

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 204 DFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRALGL----------- 272
Cdd:PLN02934  197 NCWNDFQKQMSTQVFIFCDKPKDANLIVISFRGTEPFDADDWGTDFDYSWYEIPKVGKVHMGFLEAMGLgnrddtttfqt 276

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 273 ----------QKDGWPKENISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPAILAIHGEDELLDKLEG 342
Cdd:PLN02934  277 slqtkatselKEEESKKNLLEMVERSAYYAVRSKLKSLLKEHKNAKFVVTGHSLGGALAILFPTVLVLQEETEVMKRLLG 356

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 343 IYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFDDKYlFSYKHYGPCNSFNSLYKGKVREDAPNANYFN 422
Cdd:PLN02934  357 VYTFGQPRIGNRQLGKFMEAQLNYPVPRYFRVVYCNDLVPRLPYDDKT-FLYKHFGVCLYYDSRYFGQKMDEEPDRNPFG 435

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1063693864 423 LLWLIPQLLTGLWEFIRSFILQFWKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLG 485
Cdd:PLN02934  436 LRNAISAHLNAVWELWRSFIMGYTHGPEYKEGWFSIFFRIMGLVLPGVAAHSPTDYVNSVRLG 498
Lipase_3 cd00519
Lipase (class 3). Lipases are esterases that can hydrolyze long-chain acyl-triglycerides into ...
 167-404 2.61e-49

Lipase (class 3). Lipases are esterases that can hydrolyze long-chain acyl-triglycerides into di- and monoglycerides, glycerol, and free fatty acids at a water/lipid interface. A typical feature of lipases is "interfacial activation," the process of becoming active at the lipid/water interface, although several examples of lipases have been identified that do not undergo interfacial activation . The active site of a lipase contains a catalytic triad consisting of Ser - His - Asp/Glu, but unlike most serine proteases, the active site is buried inside the structure. A "lid" or "flap" covers the active site, making it inaccessible to solvent and substrates. The lid opens during the process of interfacial activation, allowing the lipid substrate access to the active site.


Pssm-ID: 238287 [Multi-domain]  Cd Length: 229  Bit Score: 169.19  E-value: 2.61e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 167 EYKSMLSIMASKISYESKPYITS--VVKNTWKMDLVGNYDFYNAFQESKLTQAFVFKTSSTNpdLIVVSFRGTEPFEaaD 244
Cdd:cd00519     2 YEKLKYYAKLAAAAYCVDANILAkaVVFADIALLNVFSPDKLLKTDKQYDTQGYVAVDHDRK--TIVIAFRGTVSLA--D 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 245 WCTDLDLSWYEMK----NVGKVHAGFSRALGLQKDGWPKENISLLHQYAYYTIrqmlrdklgrnknlkyILTGHSLGGAL 320
Cdd:cd00519    78 WLTDLDFSPVPLDpplcSGGKVHSGFYSAYKSLYNQVLPELKSALKQYPDYKI----------------IVTGHSLGGAL 141

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 321 AALFPAILAIHGEDELLdkleGIYTFGQPRVGDEDFGEFMKGVVKKHgieyERFVYNNDVVPRVPFDDKYL-FSYKHYGP 399
Cdd:cd00519   142 ASLLALDLRLRGPGSDV----TVYTFGQPRVGNAAFAEYLESTKGRV----YRVVHGNDIVPRLPPGSLTPpEGYTHVGT 213


                  ....*
gi 1063693864 400 CNSFN 404
Cdd:cd00519   214 EVWID 218
Lipase_3 pfam01764
Lipase (class 3);
231-388 9.50e-36

Lipase (class 3);


Pssm-ID: 396362 [Multi-domain]  Cd Length: 139  Bit Score: 129.69  E-value: 9.50e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 231 VVSFRGTEpfEAADWCTDLDLSWYEMKNV----GKVHAGFSRALGLqkdgwpkenisllhqyAYYTIRQMLRDKLGRNKN 306
Cdd:pfam01764   1 VVAFRGTN--SILDWLTDFDFSLTPFKDFflggGKVHSGFLSAYTS----------------VREQVLAELKRLLEKYPD 62

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 307 LKYILTGHSLGGALAALFPAILAIHGedELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHgieYERFVYNNDVVPRVPF 386
Cdd:pfam01764  63 YSIVVTGHSLGGALASLAALDLVENG--LRLSSRVTVVTFGQPRVGNLEFAKLHDSQGPKF---SYRVVHQRDIVPRLPP 137


                  ..
gi 1063693864 387 DD 388
Cdd:pfam01764 138 IV 139
Lip2 COG3675
Predicted lipase [Lipid transport and metabolism];
226-420 2.15e-33

Predicted lipase [Lipid transport and metabolism];


Pssm-ID: 442891 [Multi-domain]  Cd Length: 266  Bit Score: 127.56  E-value: 2.15e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 226 NPDLIVVSFRGTEpfEAADWCTDLDL---SWYEMKNVGKVHAGFSRAlglqkdgwpkenisllhqyaYYTIRQMLRDKLG 302
Cdd:COG3675    25 SDDEVIVAFRGTE--SLTDWLTNLNAaqvPYPFAKTGGKVHRGFYRA--------------------LQSLRELLEDALR 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 303 RNKNLKYI-LTGHSLGGALAALFpailAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHgieYeRFVYNNDVV 381
Cdd:COG3675    83 PLSPGKRLyVTGHSLGGALATLA----AADLERNYIFPVRGLYTFGQPRVGDRSFAKYYNLHVPNS---Y-RIVNNNDIV 154

                         170       180       190
                  ....*....|....*....|....*....|....*....
gi 1063693864 382 PRVPFddkYLFSYKHYGPCNSFNSLyKGKVREDAPNANY 420
Cdd:COG3675   155 PLLPP---VWMGYDHVGKLLWLDSL-RKDMLTDHSMDNY 189
Lipase cd00741
Lipase. Lipases are esterases that can hydrolyze long-chain acyl-triglycerides into di- and ...
 288-419 1.78e-24

Lipase. Lipases are esterases that can hydrolyze long-chain acyl-triglycerides into di- and monoglycerides, glycerol, and free fatty acids at a water/lipid interface. A typical feature of lipases is "interfacial activation", the process of becoming active at the lipid/water interface, although several examples of lipases have been identified that do not undergo interfacial activation . The active site of a lipase contains a catalytic triad consisting of Ser - His - Asp/Glu, but unlike most serine proteases, the active site is buried inside the structure. A "lid" or "flap" covers the active site, making it inaccessible to solvent and substrates. The lid opens during the process of interfacial activation, allowing the lipid substrate access to the active site.


Pssm-ID: 238382 [Multi-domain]  Cd Length: 153  Bit Score: 99.11  E-value: 1.78e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 288 YAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPAILAIHGedelLDKLEGIYTFGQPRVGDEDFGEfmKGVVKKH 367
Cdd:cd00741     9 SLANLVLPLLKSALAQYPDYKIHVTGHSLGGALAGLAGLDLRGRG----LGRLVRVYTFGPPRVGNAAFAE--DRLDPSD 82

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1063693864 368 GIEYERFVYNNDVVPRVPFddkYLFSYKHYGPCNSFNSLYKGKVREDAPNAN 419
Cdd:cd00741    83 ALFVDRIVNDNDIVPRLPP---GGEGYPHGGAEFYINGGKSQPGCCKNVLEA 131
PLN02310 PLN02310
triacylglycerol lipase
 230-385 5.53e-08

triacylglycerol lipase


Pssm-ID: 215176  Cd Length: 405  Bit Score: 54.99  E-value: 5.53e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 230 IVVSFRGTepFEAADWCTDLDLSWYEMKNVG-KVHAGFSRALGlQKDGWPKENISLLHQYAYYTIRQMLRDKLGRNKNLK 308
Cdd:PLN02310  134 IMVAWRGT--VAPSEWFLDLETKLEHIDNTNvKVQEGFLKIYK-SKDESTRYNKLSASEQVMQEVKRLVNFYRGKGEEVS 210

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1063693864 309 YILTGHSLGGALAALFpailAIHGEDELLDKLEGIYTFGQPRVGDEDFGEfmkgVVKKHGIEYERFVYNNDVVPRVP 385
Cdd:PLN02310  211 LTVTGHSLGGALALLN----AYEAATTIPDLFVSVISFGAPRVGNIAFKE----KLNELGVKTLRVVVKQDKVPKLP 279
PLN03037 PLN03037
lipase class 3 family protein; Provisional
 230-385 8.87e-08

lipase class 3 family protein; Provisional


Pssm-ID: 215547  Cd Length: 525  Bit Score: 54.57  E-value: 8.87e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 230 IVVSFRGTepFEAADWCTDL--DLSWYEM-----KNVGKVHAGFSRALGLQKDGWPKENISLLHQyAYYTIRQMLRDKLG 302
Cdd:PLN03037  237 IVVAWRGT--VAPTEWFMDLrtSLEPFDCdgdhgKNVVKVQSGFLSIYKSKSELTRYNKLSASEQ-VMEEVKRLVNFFKD 313

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 303 RNKNLKYILTGHSLGGALAAL--FPAILAIHGEDELldkleGIYTFGQPRVGDEDFGEFMKGVvkkhGIEYERFVYNNDV 380
Cdd:PLN03037  314 RGEEVSLTITGHSLGGALALLnaYEAARSVPALSNI-----SVISFGAPRVGNLAFKEKLNEL----GVKVLRVVNKQDI 384


                  ....*
gi 1063693864 381 VPRVP 385
Cdd:PLN03037  385 VPKLP 389
PLN02408 PLN02408
phospholipase A1
 292-385 1.16e-06

phospholipase A1


Pssm-ID: 215228  Cd Length: 365  Bit Score: 50.61  E-value: 1.16e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 292 TIRQMLRDKLGR------NKNLKYILTGHSLGGALaalfpAILAIHGEDELLDK--LEGIYTFGQPRVGDEDFGEFMkgv 363
Cdd:PLN02408  179 SLQEMVREEIARllqsygDEPLSLTITGHSLGAAL-----ATLTAYDIKTTFKRapMVTVISFGGPRVGNRSFRRQL--- 250

                          90       100
                  ....*....|....*....|..
gi 1063693864 364 vKKHGIEYERFVYNNDVVPRVP 385
Cdd:PLN02408  251 -EKQGTKVLRIVNSDDVITKVP 271
PLN02802 PLN02802
triacylglycerol lipase
 312-398 1.70e-06

triacylglycerol lipase


Pssm-ID: 215432  Cd Length: 509  Bit Score: 50.54  E-value: 1.70e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 312 TGHSLGGALAALFPailaihgeDELLDKLE-----GIYTFGQPRVGDEDFGEfmkgVVKKHGIEYERFVYNNDVVPRVP- 385
Cdd:PLN02802  335 TGHSLGAALALLVA--------DELATCVPaappvAVFSFGGPRVGNRAFAD----RLNARGVKVLRVVNAQDVVTRVPg 402

                          90
                  ....*....|....*.
gi 1063693864 386 ---FDDKYLFSYKHYG 398
Cdd:PLN02802  403 iapREELHKWAYAHVG 418
PLN02324 PLN02324
triacylglycerol lipase
 230-423 2.71e-06

triacylglycerol lipase


Pssm-ID: 177958  Cd Length: 415  Bit Score: 49.63  E-value: 2.71e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 230 IVVSFRGT-EPFEaadWCTDLDLSW---------YEMKNVGKVHAGFSRALGLQKDGWPKENISLLHQyAYYTIRQMLrd 299
Cdd:PLN02324  134 IVVAWRGTlQPYE---WANDFDFPLesaisvfpvTDPKDNPRIGSGWLDIYTASDSRSPYDTTSAQEQ-VQGELKRLL-- 207

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 300 KLGRNKNLKYILTGHSLGGALAALFPAILaIHGE-DELLDKLEG------IYTFGQPRVGDEDFGEFMKGVVKKHGIeye 372
Cdd:PLN02324  208 ELYKNEEISITFTGHSLGAVMSVLSAADL-VYGKkNKINISLQKkqvpitVFAFGSPRIGDHNFKNLVDSLQPLNIL--- 283

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1063693864 373 RFVYNNDVVPRVPfddkyLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNL 423
Cdd:PLN02324  284 RIVNVPDVAPHYP-----LLLYTEIGEVLEINTLNSTYLKRSLNFRNYHNL 329
PLN02753 PLN02753
triacylglycerol lipase
 287-385 1.30e-04

triacylglycerol lipase


Pssm-ID: 178354  Cd Length: 531  Bit Score: 44.70  E-value: 1.30e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 287 QYAYYTIRQMLRDKLGR---------NKNLKYILTGHSLGGALAALFPAILAIHGedelLDKLEG-------IYTFGQPR 350
Cdd:PLN02753  283 KFAKFSAREQILTEVKRlveehgdddDSDLSITVTGHSLGGALAILSAYDIAEMG----LNRSKKgkvipvtVLTYGGPR 358

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 1063693864 351 VGDEDFGEFMKGVvkkhGIEYERFVYNNDVVPRVP 385
Cdd:PLN02753  359 VGNVRFKDRMEEL----GVKVLRVVNVHDVVPKSP 389
CVT17 COG5153
Putative lipase ATG15 (essential for vacuolar disintegration of autophagic bodies) ...
 231-499 1.91e-04

Putative lipase ATG15 (essential for vacuolar disintegration of autophagic bodies) [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 444061  Cd Length: 405  Bit Score: 43.85  E-value: 1.91e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 231 VVSFRGTEpfEAADWCTDLD--LSWYEMKN-------VGKVHAGFSRALGLQKDGWPKENISLLHQYayytirqmlrdkL 301
Cdd:COG5153    48 IIAFRGTQ--GKPDWKTDINasLHDYDEKNkeadeklPLQVHEGFEQYAAQVMDLDYDGAEELAAEV------------K 113

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 302 GRNKNLKYILTGHSLGGALA---ALFPAILAIhgedelldklegiyTFGQPRVGDEDFGEfmkgvVKKHGIEYERFVYNN 378
Cdd:COG5153   114 KQYPDAELSLTGHSLGGALAslvAVATGLSKV--------------TFAAPGSGNHALAD-----DLGKRIDAGEFVKSL 174

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 379 DVVPR--VPFDDKYLFSYKHYGPCNSFNSLYKgkvredAPNANYFNLLWLIPqlLTGLWEFIRSFILQFWKGDEYKEN-- 454
Cdd:COG5153   175 DAVAGpgDSFFGGAFKQFGHVGEFNHIGNDSG------TAHPAKFTIPLAIP--LERDSEDGALFLGLKQKPILAQPIgk 246

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1063693864 455 ------WLMRFVRVV-GIVFPGGSNHFPFDYvnstrLGGLVRPPPTTTPEDK 499
Cdd:COG5153   247 kmmqadAFTKFEDSFdGGEKPDGGEFFLDDE-----NGMLPRPLVLDDGHEK 293
PLN02571 PLN02571
triacylglycerol lipase
 230-386 2.04e-03

triacylglycerol lipase


Pssm-ID: 215309  Cd Length: 413  Bit Score: 40.64  E-value: 2.04e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 230 IVVSFRGT-EPFEaadWCTDLDLswyemknvGKVHAgfSRALGlQKDGWPKenislLHQ--YAYYT------------IR 294
Cdd:PLN02571  147 IVIAWRGTvQTLE---WVNDFEF--------NLVSA--SKIFG-ESNDQPK-----VHQgwYSIYTsdderspfnktsAR 207

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 295 QMLRDKLGR------NKNLKYILTGHSLGGALAALFPAILAIHGEDELLDKLE-----GIYTFGQPRVGDEDFGEFMKG- 362
Cdd:PLN02571  208 DQVLNEVGRlvekykDEEISITICGHSLGAALATLNAVDIVANGFNRSKSRPNkscpvTAFVFASPRVGDSDFKKLFSGl 287

                         170       180
                  ....*....|....*....|....*...
gi 1063693864 363 ----VVKKHGIEyerfvynnDVVPRVPF 386
Cdd:PLN02571  288 kdlrVLRVRNLP--------DVIPNYPL 307
PLN02719 PLN02719
triacylglycerol lipase
 305-385 2.34e-03

triacylglycerol lipase


Pssm-ID: 178321  Cd Length: 518  Bit Score: 40.46  E-value: 2.34e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063693864 305 KNLKYILTGHSLGGALAALFPAILAIHGEDELLD-KLEGI--YTFGQPRVGDEDFGEfmkgVVKKHGIEYERFVYNNDVV 381
Cdd:PLN02719  296 EELSITVTGHSLGGALAVLSAYDVAEMGLNRTRKgKVIPVtaFTYGGPRVGNIRFKE----RIEELGVKVLRVVNEHDVV 371


                  ....
gi 1063693864 382 PRVP 385
Cdd:PLN02719  372 AKSP 375
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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