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Conserved domains on  [gi|442614627|ref|NP_001259094|]
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erect wing, isoform N [Drosophila melanogaster]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Nrf1_DNA-bind super family cl11131
NLS-binding and DNA-binding and dimerization domains of Nrf1; In Drosophila, the erect wing ...
 145-316 2.03e-99

NLS-binding and DNA-binding and dimerization domains of Nrf1; In Drosophila, the erect wing (ewg) protein is required for proper development of the central nervous system and the indirect flight muscles. The fly ewg gene encodes a novel DNA-binding domain that is also found in four genes previously identified in sea urchin, chicken, zebrafish, and human. Nuclear respiratory factor-1 is a transcriptional activator that has been implicated in the nuclear control of respiratory chain expression in vertebrates. The first 26 amino acids of nuclear respiratory factor-1 are required for the binding of dynein light chain. The interaction with dynein light chain is observed for both ewg and Nrf-1, transcription factors that are structurally and functionally similar between humans and Drosophila. The highest level of expression of both ewg and Nrf-1 was found in the central nervous system, somites, first branchial arch, optic vesicle, and otic vesicle. In the mouse Nrf-1 protein, Swiss-Prot:Q8C4C0, there is also an NLS domain at 88-116, and a DNA binding and dimerization domain at 127-282. Ewg is a site-specific transcriptional activator, and evolutionarily conserved regions of ewg contribute both positively and negatively to transcriptional activity.


The actual alignment was detected with superfamily member pfam10491:

Pssm-ID: 463112  Cd Length: 213  Bit Score: 292.41  E-value: 2.03e-99
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442614627  145 SSKKRKRPHCFETNPSVRKRQQNRLLRKLRAIIYEFTGRVGKQAVVLVATPGKPNTSYKVFGAKPLEDVLRNLKNIVMDE 224
Cdd:pfam10491  13 TGKKRKRPHVFESNPSIRKRQQTRLLRKLRATLDEYTTRVGQQAVVLCCSPSKPNPVFKVFGAAPLENVIRNYKSVILQD 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442614627  225 LDNALAQQA-PPPPQDDPSLFELPGLVIDGIPTPVEKMTQAQLRAFIPLMLKYSTGRGKPGWGRESTRPPWWPKELPWAN 303
Cdd:pfam10491  93 LESALAEQApPPQEVENPGLFELPPLVIDGIPTPVDKMTQAQLRAFIPEMLKYSTGRGKPGWGKESCRPVWWPKDVPWAN 172

                         170
                  ....*....|...
gi 442614627  304 VRMDARSEDDKQK 316
Cdd:pfam10491 173 VRSDVRTEEQKQR 185
 
Name Accession Description Interval E-value
Nrf1_DNA-bind pfam10491
NLS-binding and DNA-binding and dimerization domains of Nrf1; In Drosophila, the erect wing ...
 145-316 2.03e-99

NLS-binding and DNA-binding and dimerization domains of Nrf1; In Drosophila, the erect wing (ewg) protein is required for proper development of the central nervous system and the indirect flight muscles. The fly ewg gene encodes a novel DNA-binding domain that is also found in four genes previously identified in sea urchin, chicken, zebrafish, and human. Nuclear respiratory factor-1 is a transcriptional activator that has been implicated in the nuclear control of respiratory chain expression in vertebrates. The first 26 amino acids of nuclear respiratory factor-1 are required for the binding of dynein light chain. The interaction with dynein light chain is observed for both ewg and Nrf-1, transcription factors that are structurally and functionally similar between humans and Drosophila. The highest level of expression of both ewg and Nrf-1 was found in the central nervous system, somites, first branchial arch, optic vesicle, and otic vesicle. In the mouse Nrf-1 protein, Swiss-Prot:Q8C4C0, there is also an NLS domain at 88-116, and a DNA binding and dimerization domain at 127-282. Ewg is a site-specific transcriptional activator, and evolutionarily conserved regions of ewg contribute both positively and negatively to transcriptional activity.


Pssm-ID: 463112  Cd Length: 213  Bit Score: 292.41  E-value: 2.03e-99
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442614627  145 SSKKRKRPHCFETNPSVRKRQQNRLLRKLRAIIYEFTGRVGKQAVVLVATPGKPNTSYKVFGAKPLEDVLRNLKNIVMDE 224
Cdd:pfam10491  13 TGKKRKRPHVFESNPSIRKRQQTRLLRKLRATLDEYTTRVGQQAVVLCCSPSKPNPVFKVFGAAPLENVIRNYKSVILQD 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442614627  225 LDNALAQQA-PPPPQDDPSLFELPGLVIDGIPTPVEKMTQAQLRAFIPLMLKYSTGRGKPGWGRESTRPPWWPKELPWAN 303
Cdd:pfam10491  93 LESALAEQApPPQEVENPGLFELPPLVIDGIPTPVDKMTQAQLRAFIPEMLKYSTGRGKPGWGKESCRPVWWPKDVPWAN 172

                         170
                  ....*....|...
gi 442614627  304 VRMDARSEDDKQK 316
Cdd:pfam10491 173 VRSDVRTEEQKQR 185
 
Name Accession Description Interval E-value
Nrf1_DNA-bind pfam10491
NLS-binding and DNA-binding and dimerization domains of Nrf1; In Drosophila, the erect wing ...
 145-316 2.03e-99

NLS-binding and DNA-binding and dimerization domains of Nrf1; In Drosophila, the erect wing (ewg) protein is required for proper development of the central nervous system and the indirect flight muscles. The fly ewg gene encodes a novel DNA-binding domain that is also found in four genes previously identified in sea urchin, chicken, zebrafish, and human. Nuclear respiratory factor-1 is a transcriptional activator that has been implicated in the nuclear control of respiratory chain expression in vertebrates. The first 26 amino acids of nuclear respiratory factor-1 are required for the binding of dynein light chain. The interaction with dynein light chain is observed for both ewg and Nrf-1, transcription factors that are structurally and functionally similar between humans and Drosophila. The highest level of expression of both ewg and Nrf-1 was found in the central nervous system, somites, first branchial arch, optic vesicle, and otic vesicle. In the mouse Nrf-1 protein, Swiss-Prot:Q8C4C0, there is also an NLS domain at 88-116, and a DNA binding and dimerization domain at 127-282. Ewg is a site-specific transcriptional activator, and evolutionarily conserved regions of ewg contribute both positively and negatively to transcriptional activity.


Pssm-ID: 463112  Cd Length: 213  Bit Score: 292.41  E-value: 2.03e-99
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442614627  145 SSKKRKRPHCFETNPSVRKRQQNRLLRKLRAIIYEFTGRVGKQAVVLVATPGKPNTSYKVFGAKPLEDVLRNLKNIVMDE 224
Cdd:pfam10491  13 TGKKRKRPHVFESNPSIRKRQQTRLLRKLRATLDEYTTRVGQQAVVLCCSPSKPNPVFKVFGAAPLENVIRNYKSVILQD 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442614627  225 LDNALAQQA-PPPPQDDPSLFELPGLVIDGIPTPVEKMTQAQLRAFIPLMLKYSTGRGKPGWGRESTRPPWWPKELPWAN 303
Cdd:pfam10491  93 LESALAEQApPPQEVENPGLFELPPLVIDGIPTPVDKMTQAQLRAFIPEMLKYSTGRGKPGWGKESCRPVWWPKDVPWAN 172

                         170
                  ....*....|...
gi 442614627  304 VRMDARSEDDKQK 316
Cdd:pfam10491 173 VRSDVRTEEQKQR 185
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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