NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|186530601|ref|NP_001119399|]
View 

DNA replication protein-like protein [Arabidopsis thaliana]

Protein Classification

DNA replication complex GINS protein SLD5 family protein( domain architecture ID 10184927)

DNA replication complex GINS protein SLD5 family protein simila rto alpha-helical region of DNA replication complex GINS protein SLD5, which is a component of the heterotetrameric GINS complex that is essential for both the initiation and elongation stages of eukaryotic DNA replication

Gene Ontology:  GO:0006261
PubMed:  22918584|20070258

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
GINS_A_Sld5 cd11711
Alpha-helical domain of GINS complex protein Sld5; Sld5 is a component of GINS tetrameric ...
 21-139 3.32e-47

Alpha-helical domain of GINS complex protein Sld5; Sld5 is a component of GINS tetrameric protein complex, and within the complex Sld5 interacts with Psf1 via its N-terminal A-domain, and with Psf2 through a combination of the A and B domains. Sld5 in Drosophila is required for normal cell cycle progression and the maintenance of genomic integrity. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits are homologous and homologs are also found in the archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


:

Pssm-ID: 212549  Cd Length: 119  Bit Score: 150.45  E-value: 3.32e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186530601  21 DVELLKRAWRNEKAAPEILQYEGALVDRAKEQIELVEETIEDYVENGIDPLVVSLYQMDLDRAQFLLRSYLRVRLLKIEK 100
Cdd:cd11711    1 DLELLKRAWVNEKFAPELLPYQEELVERVLEQIEQQEENLEELKASEKDDLRLSLHEMELERIRFLLRSYLRTRLQKIEK 80

                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 186530601 101 FMFHNLNSEEAERRLSEQEKVFATRCADDLAKHFEETVL 139
Cdd:cd11711   81 YALHILSEEELPSRLSPEELEFAKRYAELLENHLKSSVL 119
 
Name Accession Description Interval E-value
GINS_A_Sld5 cd11711
Alpha-helical domain of GINS complex protein Sld5; Sld5 is a component of GINS tetrameric ...
 21-139 3.32e-47

Alpha-helical domain of GINS complex protein Sld5; Sld5 is a component of GINS tetrameric protein complex, and within the complex Sld5 interacts with Psf1 via its N-terminal A-domain, and with Psf2 through a combination of the A and B domains. Sld5 in Drosophila is required for normal cell cycle progression and the maintenance of genomic integrity. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits are homologous and homologs are also found in the archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


Pssm-ID: 212549  Cd Length: 119  Bit Score: 150.45  E-value: 3.32e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186530601  21 DVELLKRAWRNEKAAPEILQYEGALVDRAKEQIELVEETIEDYVENGIDPLVVSLYQMDLDRAQFLLRSYLRVRLLKIEK 100
Cdd:cd11711    1 DLELLKRAWVNEKFAPELLPYQEELVERVLEQIEQQEENLEELKASEKDDLRLSLHEMELERIRFLLRSYLRTRLQKIEK 80

                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 186530601 101 FMFHNLNSEEAERRLSEQEKVFATRCADDLAKHFEETVL 139
Cdd:cd11711   81 YALHILSEEELPSRLSPEELEFAKRYAELLENHLKSSVL 119
COG5086 COG5086
Uncharacterized conserved protein [Function unknown];
17-149 4.79e-14

Uncharacterized conserved protein [Function unknown];


Pssm-ID: 227418 [Multi-domain]  Cd Length: 218  Bit Score: 67.68  E-value: 4.79e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186530601  17 MSTSDVELLKRAWRNEKAAPEILQYEGALVDRAKEQIELVEETIEDYVENGI-DPLVVSLYQMDLDRAQFLLRSYLRVRL 95
Cdd:COG5086   19 MSFPDLNDLILAWQNERSTPRLLPYALHLIDRFLDRISEQTEHVKIALGKSIaKNNESILMETELERVKFVVRSYIRTRL 98

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 186530601  96 LKIEKFMFHNLNSEEAERRLSEQEKVFATRCADDLAKHFEETVLLKLPENYQSV 149
Cdd:COG5086   99 KKIDKNLYYDLSDENSLILLSGGELAYYEKHSLIYIDLLKDSDLYVTPQKFHKV 152
Sld5 pfam05916
GINS complex protein; The eukaryotic GINS complex is essential for the initiation and ...
 57-120 1.08e-04

GINS complex protein; The eukaryotic GINS complex is essential for the initiation and elongation phases of DNA replication. It consists of four paralogous protein subunits (Sld5, Psf1, Psf2 and Psf3), all of which are included in this family. The GINS complex is conserved from yeast to humans, and has been shown in human to bind directly to DNA primase.


Pssm-ID: 399129  Cd Length: 105  Bit Score: 39.79  E-value: 1.08e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186530601   57 EETIEDYVENGIDPLVVSLYQMDLDR---AQFLLRSYLRVRLLKIEKFMFHNLNSEEAE---RRLSEQEK 120
Cdd:pfam05916  36 DETKNDLSLLPPYFYELAVLLLELERddeLKRLLRDYLRIRLAKIRKLAWHLNGSLSPSdllDNLSEEEL 105
 
Name Accession Description Interval E-value
GINS_A_Sld5 cd11711
Alpha-helical domain of GINS complex protein Sld5; Sld5 is a component of GINS tetrameric ...
 21-139 3.32e-47

Alpha-helical domain of GINS complex protein Sld5; Sld5 is a component of GINS tetrameric protein complex, and within the complex Sld5 interacts with Psf1 via its N-terminal A-domain, and with Psf2 through a combination of the A and B domains. Sld5 in Drosophila is required for normal cell cycle progression and the maintenance of genomic integrity. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits are homologous and homologs are also found in the archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


Pssm-ID: 212549  Cd Length: 119  Bit Score: 150.45  E-value: 3.32e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186530601  21 DVELLKRAWRNEKAAPEILQYEGALVDRAKEQIELVEETIEDYVENGIDPLVVSLYQMDLDRAQFLLRSYLRVRLLKIEK 100
Cdd:cd11711    1 DLELLKRAWVNEKFAPELLPYQEELVERVLEQIEQQEENLEELKASEKDDLRLSLHEMELERIRFLLRSYLRTRLQKIEK 80

                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 186530601 101 FMFHNLNSEEAERRLSEQEKVFATRCADDLAKHFEETVL 139
Cdd:cd11711   81 YALHILSEEELPSRLSPEELEFAKRYAELLENHLKSSVL 119
COG5086 COG5086
Uncharacterized conserved protein [Function unknown];
17-149 4.79e-14

Uncharacterized conserved protein [Function unknown];


Pssm-ID: 227418 [Multi-domain]  Cd Length: 218  Bit Score: 67.68  E-value: 4.79e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186530601  17 MSTSDVELLKRAWRNEKAAPEILQYEGALVDRAKEQIELVEETIEDYVENGI-DPLVVSLYQMDLDRAQFLLRSYLRVRL 95
Cdd:COG5086   19 MSFPDLNDLILAWQNERSTPRLLPYALHLIDRFLDRISEQTEHVKIALGKSIaKNNESILMETELERVKFVVRSYIRTRL 98

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 186530601  96 LKIEKFMFHNLNSEEAERRLSEQEKVFATRCADDLAKHFEETVLLKLPENYQSV 149
Cdd:COG5086   99 KKIDKNLYYDLSDENSLILLSGGELAYYEKHSLIYIDLLKDSDLYVTPQKFHKV 152
Sld5 pfam05916
GINS complex protein; The eukaryotic GINS complex is essential for the initiation and ...
 57-120 1.08e-04

GINS complex protein; The eukaryotic GINS complex is essential for the initiation and elongation phases of DNA replication. It consists of four paralogous protein subunits (Sld5, Psf1, Psf2 and Psf3), all of which are included in this family. The GINS complex is conserved from yeast to humans, and has been shown in human to bind directly to DNA primase.


Pssm-ID: 399129  Cd Length: 105  Bit Score: 39.79  E-value: 1.08e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186530601   57 EETIEDYVENGIDPLVVSLYQMDLDR---AQFLLRSYLRVRLLKIEKFMFHNLNSEEAE---RRLSEQEK 120
Cdd:pfam05916  36 DETKNDLSLLPPYFYELAVLLLELERddeLKRLLRDYLRIRLAKIRKLAWHLNGSLSPSdllDNLSEEEL 105
GINS_A_archaea cd11714
Alpha-helical domain of archaeal GINS complex proteins; The GINS complex is involved in ...
 22-122 1.05e-03

Alpha-helical domain of archaeal GINS complex proteins; The GINS complex is involved in replication of archaeal and eukayotic genomes. The archaeal DNA replication system is a simplified version of that of the eukaryotes. Like its eukaryotic counterpart, the archaeal GINS complex is tetrameric, but instead of four different subunits (Sld5, Psf1, Psf2 and Psf3) it consists of two different proteins named Gins51 and Gins23. All GINS subunits are homologs and they can be classified into two groups. One group (the eukayotic Sld5 and Psf1, as well as the archaeal Gins51) has the alpha-helical (A) domain at the N-terminus and the beta-strand domain (B) at the C-terminus (this arrangement is called ABtype). The arrangement of the A and B domains is reversed in the second group (eukaryotic Psf2 and Psf3 and archaeal Gins23, also referred to as BAtype). The overall fold of each archaeal subunit and the overall tetrameric assembly of GINS are similar, but the relative locations of the C-terminal small domains are different with respect to the alpha helical domain characterized by this model, resulting in different subunit contacts in the archaeal GINS complex.Some archaea may have a homotetrameric GINS complex (4 copies of an AB-type module).


Pssm-ID: 212552  Cd Length: 105  Bit Score: 36.93  E-value: 1.05e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186530601  22 VELLKRAWRNEKAAPEILQYEGALVDRAKEQIELVEETIEDYVEngidplvVSLYQMDLDRAQFLLRSYLRVRLLKIEKF 101
Cdd:cd11714    1 IEELRKILFKERKSASLQKLPEDFYERAKEYIKELEEEARKSPE-------AQELLDELEKAKSTLRELFRLRLRKIVKL 73

                         90       100
                 ....*....|....*....|.
gi 186530601 102 MFHNLNSEEAERRLSEQEKVF 122
Cdd:cd11714   74 ALLSAEDREELENLTPEEKEL 94
GINS_A cd11581
Alpha-helical domain of GINS complex proteins; Sld5, Psf1, Psf2 and Psf3; The GINS complex is ...
 22-112 7.05e-03

Alpha-helical domain of GINS complex proteins; Sld5, Psf1, Psf2 and Psf3; The GINS complex is involved in both initiation and elongation stages of eukaryotic chromosome replication, with GINS being the component that most likely serves as the replicative helicase that unwinds duplex DNA ahead of the moving replication fork. In eukaryotes, GINS is a tetrameric arrangement of four subunits Sld5, Psf1, Psf2 and Psf3. The GINS complex has been found in eukaryotes and archaea, but not in bacteria. The four subunits of the complex are homologous and consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


Pssm-ID: 212547  Cd Length: 103  Bit Score: 34.78  E-value: 7.05e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186530601  22 VELLKRAWRNEKAAPEILQYEGALVDRAKEQIELVEETIEDYVENgidPLVVSLYQMDLDRAQFLLRSYLRVRLLKIEKF 101
Cdd:cd11581    1 VEKLAEAIHNERKSENLLPPPPDFMEEVKELLHRLSKRQRSETQE---ILEHRLKILELSYIRDLLKTYLNSRLDKIRDL 77

                         90
                 ....*....|.
gi 186530601 102 MFHNLNSEEAE 112
Cdd:cd11581   78 SPEFLPSNLTE 88
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH