transcription termination factor Rho; Provisional

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633    4 RRRHRDSPVRDVKTKIKQERLSPARPPRARRHSSGSSRDSSSPAQRRRDSRSPVRRKDRSPARRDRSSGRQQERSPRPRR 83
Cdd:PRK12678  80 AARAAAAARQAEQPAAEAAAAKAEAAPAARAAAAAAAEAASAPEAAQARERRERGEAARRGAARKAGEGGEQPATEARAD 159

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633   84 SRspHRNTEARIKREQDDPRGSESHRHRERRDDSNDRRLKREAERPRQRDRGREQDRQRGRiRDAHIQEQQQAERERHNE 163
Cdd:PRK12678 160 AA--ERTEEEERDERRRRGDREDRQAEAERGERGRREERGRDGDDRDRRDRREQGDRREER-GRRDGGDRRGRRRRRDRR 236

                        170       180
                 ....*....|....*....|....*...
gi 70887633  164 RRRENRLRQEAQSGGAEEMLEFGGENND 191
Cdd:PRK12678 237 DARGDDNREDRGDRDGDDGEGRGGRRGR 264

FHA domain; The FHA (Forkhead-associated) domain is a phosphopeptide binding motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633   259 YLLGRlRKIADIPIDHPSCSKQHAVFQYRlveftrvdgtAGRRVkpYIIDLGSGNGTYLNNQRIEPQrYYELKEKDVLKF 338
Cdd:pfam00498   1 VTIGR-SPDCDIVLDDPSVSRRHAEIRYD----------GGGRF--YLEDLGSTNGTFVNGQRLGPE-PVRLKDGDVIRL 66

Forkhead associated domain; Found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain.

                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 70887633    259 YLLGRLRKIADIPIDHPSCSKQHAVFQYRlveftrvdgtAGRRVkpYIIDLGSGNGTYLNNQRI 322
Cdd:smart00240   1 VTIGRSSEDCDIQLDGPSISRRHAVIVYD----------GGGRF--YLIDLGSTNGTFVNGKRI 52

U2 snRNP auxilliary factor, large subunit, splicing factor; These splicing factors consist of an N-terminal arginine-rich low complexity domain followed by three tandem RNA recognition motifs (pfam00076). The well-characterized members of this family are auxilliary components of the U2 small nuclear ribonuclearprotein splicing factor (U2AF). These proteins are closely related to the CC1-like subfamily of splicing factors (TIGR01622). Members of this subfamily are found in plants, metazoa and fungi.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633    58 RRKDRSPAR---RDRSSGRQQERSPRPRRSRSPHRNTEARIKREQDDPRGSESHRHRERRDDSND-RRLKREAERPRQRD 133
Cdd:TIGR01642   5 PDREREKSRgrdRDRSSERPRRRSRDRSRFRDRHRRSRERSYREDSRPRDRRRYDSRSPRSLRYSsVRRSRDRPRRRSRS 84

                          90
                  ....*....|....
gi 70887633   134 RGR-EQDRQRGRIR 146
Cdd:TIGR01642  85 VRSiEQHRRRLRDR 98

forkhead associated (FHA) domain found in Smad nuclear-interacting protein 1 (SNIP1), FHA domain-containing protein DDL, and similar proteins; SNIP1 is an FHA domain-containing protein required for pre-mRNA splicing as a component of the spliceosome. It inhibits NF-kappaB signaling by competing for its binding to the C/H1 domain of CBP/p300 transcriptional co-activators. It is involved in microRNA (miRNA) biogenesis. SNIP1 is a regulator of the cell cycle and cyclin D1 expression and may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. This family also includes Arabidopsis thaliana FHA domain-containing protein DDL and similar proteins. DDL, also called protein DAWDLE, is involved in the microRNA (miRNA) and short interfering RNA (siRNA) biogenesis. It may facilitate DCL1 to access or recognize primary miRNAs. DDL binds RNA non-specifically. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633 237 WRLYPFKNDEPLPVMYIHRQSAYLLGRLRKIADIPIDHPSCSKQHAVFQYRLVEfTRVDGTAGRRVKPYIIDLGSGNGTY 316
Cdd:cd22676   1 WRLYVFKGGEQLETIDIHRQSAYLIGRDRRVADIPLDHPSCSKQHAVIQFREVE-KRNEGDVIENIRPYIIDLGSTNGTF 79

                        90       100       110
                ....*....|....*....|....*....|..
gi 70887633 317 LNNQRIEPQRYYELKEKDVLKFGFSSREYVLL 348
Cdd:cd22676  80 LNGEKIEPRRYYELREKDVLKFGLSTREYVLL 111

forkhead associated (FHA) domain found in kanadaptin and similar proteins; Kanadaptin, also called human lung cancer oncogene 3 protein (HLC-3), kidney anion exchanger adapter protein, or solute carrier family 4 anion exchanger member 1 adapter protein (SLC4A1AP), is a nuclear protein widely expressed in mammalian tissues. It was originally isolated as a kidney Cl-/HCO3- anion exchanger 1 (kAE1)-binding protein. It is a highly mobile nucleocytoplasmic shuttling and multilocalizing protein. Its role in mammalian cells remains unclear. It contains an FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633 256 QSAYLLGRLrKIADIPIDHPSCSKQHAVFQYRLVEfTRVDGTAgrrvkpYIIDLGSGNGTYLNNQRIEPQRYYELKEKDV 335
Cdd:cd22677  21 KSFYVFGRL-PGCDVVLEHPSISRYHAVLQYRGDA-DDHDGGF------YLYDLGSTHGTFLNKQRIPPKQYYRLRVGHV 92

                        90
                ....*....|..
gi 70887633 336 LKFGFSSREYVL 347
Cdd:cd22677  93 LKFGGSTRLYIL 104

forkhead associated (FHA) domain found in Saccharomyces cerevisiae pre-mRNA leakage protein 1 (PML1) and similar proteins; PML1 is an FHA domain-containing protein required for efficient splicing and pre-mRNA nuclear retention. It is a component of the pre-mRNA retention and splicing (RES) complex composed of at least BUD13, IST3, and PML1. It contains an FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633 222 IKYNEPPEARIPKRRWRLYPFKNDEPLPVMYI--HRQSAYLLGR------LRKIADIPIDHPSCSKQHAVFQYRLVEFtr 293
Cdd:cd22681   3 DNYFDYTGPERDRTLYKLVIYRNNSKGPIEEYelNSPSCYLIGRekgestEIVVADIGIPEETCSKQHCVIQFRNVKG-- 80

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....
gi 70887633 294 vdgtagrRVKPYIIDLGSGNGTYLNNQRIEPQRYYELKEKDVLKFGFSSREYVL 347
Cdd:cd22681  81 -------ILKPYIMDLDSSNGTCLNDNVIPSSRYVELRSGDVITFSKSNDYELV 127

forkhead associated (FHA) domain superfamily; Forkhead-associated (FHA) domains are small phosphopeptide recognition modules mostly found in eubacteria and eukaryotes. It is about 95-120 residues long that fold into an 11-stranded beta-sandwich. FHA domains can mediate the recognition of phosphorylated and non-phosphorylated substrates, as well as protein oligomerization. They specifically recognize threonine phosphorylation (pThr) accompanying activation of protein serine/threonine kinases. FHA domains show diverse ligand specificity. They may recognize the pTXXD motif, the pTXXI/L motif, and TQ clusters (singly and multiply phosphorylated). In eukaryotes, FHA superfamily members include forkhead-type transcription factors, as well as other signaling proteins, such as many regulatory proteins, kinases, phosphatases, motor proteins called kinesins, and metabolic enzymes. Many of them localize to the nucleus, where they participate in establishing or maintaining cell cycle checkpoints, DNA repair, or transcriptional regulation. FHA domains play important roles in human diseases, particularly in relation to DNA damage responses and cancers. In bacteria, FHA domain-containing proteins may participate in injection of viral proteins into host cells, transmembrane transporters, and cell division. FHA domain-containing proteins rarely include more than one copy of the domain. The only exception in eukaryotes is the checkpoint kinase Rad53 from Saccharomyces cerevisiae, which harbors two FHA domains (FHA1 and FHA2) flanking a central kinase domain. The two FHA domains recognize different phosphorylated targets and function independently from one another. In contrast, Mycobacterium tuberculosis ABC transporter Rv1747 contains two FHA domains but only one of them is essential for protein function.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633 238 RLYPFKNDEPLPVMYIHRQSaYLLGRLRKiADIPIDHPSCSKQHAVFQYRlveftrvdgtaGRRVkpYIIDLGSGNGTYL 317
Cdd:cd00060   1 RLIVLDGDGGGREFPLTKGV-VTIGRSPD-CDIVLDDPSVSRRHARIEVD-----------GGGV--YLEDLGSTNGTFV 65

                        90       100
                ....*....|....*....|..
gi 70887633 318 NNQRIEPQryYELKEKDVLKFG 339
Cdd:cd00060  66 NGKRITPP--VPLQDGDVIRLG 85

forkhead associated (FHA) domain found in Trypanosoma brucei Parvulin 42 (TbPar42) and similar proteins; TbPar42 is a nuclear protein that plays a key role in parasite cell proliferation. It exhibits an N-terminal forkhead associated (FHA)-domain and a peptidyl-prolyl-cis/trans-isomerase (PPIase) domain, both connected by a linker. Its PPIase domain adopts a parvulin fold and reflects structural elements of Pin1-type proteins but is catalytically inactive. Its FHA domain may be involved in the binding of phosphorylated target proteins. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633 247 PLPVMYIHRQSAYLLGRlRKIADIPIDHPSCSKQHAVFQYRlveftrvdgtaGRRVKPYIIDLGSGNGTYLNNQRIEPQR 326
Cdd:cd22675  19 PLPPLGLDRFPFYLFGR-SPVCDYVLEHPSISSVHAVLVFH-----------GEQKCFVLMDLGSTNGVKLNGKRIEKGR 86

                        90       100
                ....*....|....*....|.
gi 70887633 327 YYELKEKDVLKFGFSSREYVL 347
Cdd:cd22675  87 PLPLPVGSVIQFGFSARKYKV 107

forkhead associated (FHA) domain found in mediator of DNA damage checkpoint protein 1 (MDC1) and similar proteins; MDC1, also called nuclear factor with BRCT domains 1 (NFBD1), is a nuclear chromatin-associated protein that is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S and G2/M phases of the cell cycle. It directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks. MDC1 contains a forkhead-associated (FHA) domain and two BRCT domains, as well as an internal 41-amino acid repeat sequence. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633 229 EARIPKRRWRLYPFKNdeplpvmyihrqsayLLGRLRKiADIPIDHPSCSKQHAvfqyrlvEFTRVDGTAgrrvkpYIID 308
Cdd:cd22665   8 QAHGPEKDFPLYEGEN---------------VIGRDPS-CSVVLPDKSVSKQHA-------CIEVDGGTH------LIED 58

                        90       100       110
                ....*....|....*....|....*....|..
gi 70887633 309 LGSGNGTYLNNQ-RIEPQRYYELKEKDVLKFG 339
Cdd:cd22665  59 LGSTNGTRIGNKvRLKPNVRYELIDGDLLLFG 90

forkhead associated (FHA) domain found in centrosomal protein of 170 kDa protein B (Cep170B) and similar proteins; Cep170B, also called centrosomal protein 170B, plays a role in microtubule organization. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 70887633 276 SCSKQHAVFQYRLveftrvdGTAGRRVKpyiiDLGSGNGTYLNNQRIEPQRYYELKEKDVLKFGFSSREYVL 347
Cdd:cd22725  38 SVDKQHAVINYDQ-------DTDEHWVK----DLGSLNGTFVNDVRIPDQKYITLKLNDVIRFGYDSNMYVL 98

transcription termination factor Rho; Provisional

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633    4 RRRHRDSPVRDVKTKIKQERLSPARPPRARRHSSGSSRDSSSPAQRRRDSRSPVRRKDRSPARRDRSSGRQQERSPRPRR 83
Cdd:PRK12678  80 AARAAAAARQAEQPAAEAAAAKAEAAPAARAAAAAAAEAASAPEAAQARERRERGEAARRGAARKAGEGGEQPATEARAD 159

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633   84 SRspHRNTEARIKREQDDPRGSESHRHRERRDDSNDRRLKREAERPRQRDRGREQDRQRGRiRDAHIQEQQQAERERHNE 163
Cdd:PRK12678 160 AA--ERTEEEERDERRRRGDREDRQAEAERGERGRREERGRDGDDRDRRDRREQGDRREER-GRRDGGDRRGRRRRRDRR 236

                        170       180
                 ....*....|....*....|....*...
gi 70887633  164 RRRENRLRQEAQSGGAEEMLEFGGENND 191
Cdd:PRK12678 237 DARGDDNREDRGDRDGDDGEGRGGRRGR 264

forkhead associated (FHA) domain found in nibrin and similar proteins; Nibrin (NBN), also called cell cycle regulatory protein p95, or Nijmegen breakage syndrome protein 1 (NBS1), is a novel DNA double-strand break repair protein that is mutated in Nijmegen breakage syndrome. It is a component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. Nibrin modulates the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-dependent protein kinase catalytic subunit (DNA-PKcs) to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. Nibrin also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. Nibrin is a major player in the control of intra-S-phase checkpoint. This subfamily also includes Schizosaccharomyces pombe DNA repair and telomere maintenance protein Nbs1 and Arabidopsis thaliana AtNbs1. SpNbs1 is an FHA domain-containing protein required for DNA damage repair and S-phase DNA damage checkpoint. It is involved in telomere length maintenance and maintenance of chromatin structure. AtNbs1 is a component of MRN complex. It also functions in the very early stages of meiosis. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633 237 WRLYPFKNDEPLPVmYIHRQSAYLLGRlrKIADIPIDH-PSCSKQHAVFQyrLVEFTRVDGTAGRRVKPYIIDLgSGNGT 315
Cdd:cd22667   1 WWLLSEQDGAGTSY-YLLPGGEYTVGR--KDCDIIIVDdSSISRKHATLT--VLHPEANLSDPDTRPELTLKDL-SKYGT 74

                        90       100
                ....*....|....*....|....
gi 70887633 316 YLNNQRIEPQRYYELKEKDVLKFG 339
Cdd:cd22667  75 FVNGEKLKGGSEVTLKDGDVITFG 98

forkhead associated (FHA) domain found in Arabidopsis thaliana protein phosphatase 2C 70 (AtPP2C70) and similar proteins; AtPP2C70, also called kinase-associated protein phosphatase, or protein ROOT ATTENUATED GROWTH 1, dephosphorylates the serine/threonine receptor-like kinase RLK5. It may function as a signaling component in a pathway involving RLK5. It acts as a negative regulator of the CLAVATA1 signaling in plant development by binding and dephosphorylating CLAVATA1. It is also a component of a signaling pathway which mediates adaptation to NaCl stress. It contains an FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633 261 LGRLRKiADIPIDHPSCSKQHAVFqyrlvEFTRVDGtagrrvKPYIIDLGSGNGTYLNNQRIEPQ-RYYELKEKDVLKFG 339
Cdd:cd22678  27 IGRIQR-GDIALKDDEVSGKHARI-----EWNSTGS------KWELVDLGSLNGTLVNGESISPNgRPVVLSSGDVITLG 94

                ....*..
gi 70887633 340 FSSREYV 346
Cdd:cd22678  95 SETKILV 101

forkhead associated (FHA) domain found in centrosomal protein of 170 kDa (Cep170) and similar proteins; Cep170, also called Cep170A, KARP-1-binding protein, or KARP1-binding protein, is a protein that localizes to centrosomes as well as spindle microtubules and plays a role in microtubule organization and microtubule assembly. It is required for centriole subdistal appendage assembly. Cep170 is phosphorylated during M phase and interacts with Polo-like kinase 1 (Plk1). The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 70887633 276 SCSKQHAVFQYRlveftrvDGTAGRRVKpyiiDLGSGNGTYLNNQRIEPQRYYELKEKDVLKFGFSSREYVLL 348
Cdd:cd22724  38 SVDKQHAVINYD-------ASTDEHKVK----DLGSLNGTFVNDVRIPEQTYITLKLDDKLRFGYDTNLFTVV 99

forkhead associated (FHA) domain found in Saccharomyces cerevisiae meiosis-specific serine/threonine-protein kinase MEK1 and similar proteins; MEK1 (EC 2.7.11.1), also known as MRE4, is a meiosis-specific protein kinase required for chromosome synapsis and meiotic recombination. The recruitment and activation of MEK1 require the phosphorylation of the chromosome axis protein Hop1 at Thr318 (pT318), which is necessary for recognition by the MEK1 FHA domain. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633 241 PFKNDEPLPvmyIHRQSAYLLGRLRKiADIPIDHPSCSKQHAVFqyrlvEFTRVDGTAGrrVKPYIIDLgSGNGTYLNNQ 320
Cdd:cd22670   9 PGSTDIVLP---IYKNQVITIGRSPS-CDIVINDPFVSRTHCRI-----YSVQFDESSA--PLVYVEDL-SSNGTYLNGK 76

                        90
                ....*....|....*...
gi 70887633 321 RIEPQRYYELKEKDVLKF 338
Cdd:cd22670  77 LIGRNNTVLLSDGDVIEI 94

forkhead associated (FHA) domain found in forkhead-associated domain-containing protein 1 (FHAD1) and similar proteins; FHAD1, also called FHA domain-containing protein 1, is an uncharacterized FHA domain-containing protein. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633 265 RKIADIPIDHPSCSKQHAVfqyrlVEFTRVDGTagrrvkpYII-DLGSGNGTYLNNQRIEpQRYYELKEKDVLKFGFSSR 343
Cdd:cd22700  22 REGCDLVLQSPGVEEQHAV-----IEYSEQENC-------FVLqDLNTAQGTYVNDCRIQ-NAAVRLAPGDVLRFGFGGL 88

                ....*..
gi 70887633 344 EYVLLHE 350
Cdd:cd22700  89 PYELVVD 95

forkhead associated (FHA) domain found in Mycobacterium tuberculosis FHA domain-containing protein FhaA and similar proteins; FhaA regulates cell growth and peptidoglycan synthesis by binding to MviN. It may inhibit the late stages of peptidoglycan synthesis. It contains an FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633 255 RQSAYLLGRLRKiADIPIDHPSCSKQHAvfqyrlvEFTRVDGTAgrrvkpYIIDLGSGNGTYLNNQRIEPQryYELKEKD 334
Cdd:cd22668  16 REGSNIIGRGSD-ADFRLPDTGVSRRHA-------EIRWDGQVA------HLTDLGSTNGTTVNNAPVTPE--WRLADGD 79

                ....*...
gi 70887633 335 VLKFGFSS 342
Cdd:cd22668  80 VITLGHSE 87

forkhead associated (FHA) domain found in sarcolemmal membrane-associated protein (SLMAP) and similar proteins; SLMAP, also called sarcolemmal-associated protein (SLAP), is a tail-anchored protein involved in fundamental cellular processes, such as myoblast fusion, cell cycle progression, and chromosomal inheritance. It is a cardiac membrane protein that plays an important role in E-C coupling and the adrenergic response of the heart. Overexpression of the SLMAP gene has been associated with diabetes and endothelial dysfunction of macro- and micro-blood vessels. SLMAP contains an N-terminal FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 70887633 278 SKQHAVFQYRlveftrvDGtagrrvKPYIIDLGSGNGTYLNNQRI----EPQRYYELKEKDVLKFG 339
Cdd:cd22679  50 SRNHALLWYD-------DG------KFYLQDTKSSNGTFVNNQRLskgsEESEPRELHSGDIVQFG 102

forkhead associated (FHA) domain found in Saccharomyces cerevisiae DNA damage response protein kinase DUN1 and similar proteins; DUN1 is a protein kinase that controls the DNA damage response in yeast. It phosphorylates SML1 on serine residues and cooperates with the PAN deadenylation complex in the regulation of RAD5 mRNA levels and cell survival in response to replicational stress. It contains an FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633 249 PVMYIHRQSAYLLGRLRKIaDIPIDHPSCSKQHAVFQyrlVEFTRVdgtagrrvkpYIIDLGSGNGTYLNNQRIEpQRYY 328
Cdd:cd22683  13 QKISITNRNVTTIGRSRSC-DLVLSDPSISRFHAELR---LEQNGI----------NVIDNNSANGTFINGKRIK-GKTY 77

                        90
                ....*....|.
gi 70887633 329 ELKEKDVLKFG 339
Cdd:cd22683  78 ILKNGDIIVFG 88

forkhead associated (FHA) domain found in transcription factor 19 (TCF-19) and similar proteins; TCF-19, also called transcription factor SC1, was identified as a putative trans-activating factor with expression beginning at the late G1-S boundary in dividing cells. It also functions as a novel islet factor necessary for proliferation and survival in the INS-1 beta cell line. It plays an important role in susceptibility to both Type 1 Diabetes Mellitus (T1DM) and Type 2 Diabetes Mellitus (T2DM); it has been suggested that it may positively impact beta cell mass under conditions of beta cell stress and increased insulin demand. TCF-19 contains an N-terminal fork head association domain (FHA), a proline rich region, and a C-terminal plant homeodomain (PHD) finger. The FHA domain may serve as a nuclear signaling domain or as a phosphoprotein binding domain. The proline rich region is a common characteristic of trans-activating factors. The PHD finger may allow TCF-19 to interact with chromatin via methylated histone H3.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633 256 QSAYLLGRLRKIADIPIdhpsCSKQHAVFQYRLVEFTRVDGTAGRRVKPYIIDLgSGNGTYLNNQRIEPQRYYELKEKDV 335
Cdd:cd22685  27 LCEYRIGRNPEVCDVFL----CSSQHPNLISREHAEIHAERDGNGNWKVLIEDR-STNGTYVNDVRLQDGQRRELSDGDT 101

                        90
                ....*....|....*
gi 70887633 336 LKFGFSSREYVLLHE 350
Cdd:cd22685 102 ITFGHKNGRRVKQWP 116

second forkhead associated (FHA) domain found in Mycobacterium tuberculosis ABC transporter ATP-binding/permease protein Rv1747 and similar proteins; Rv1747 is a putative ATP-binding cassette (ABC) transporter involved in the translocation of an unknown substrate across the membrane. It is required for normal virulent infection by M. tuberculosis. Rv1747 has a cytoplasmic regulatory module consisting of two pThr-interacting forkhead-associated (FHA) domains connected by a conformationally disordered linker with two phospho-acceptor threonines (pThr). Recruitment and phosphorylation of Rv1747 depend on the interaction between its two non-redundant FHA domains and the autophosphorylated form of serine/threonine protein kinase PknF. This model corresponds to the second FHA domain, which has a circularly permuted FHA domain fold with a conserved pThr-binding interface.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 70887633 269 DIPIDHPSCSKQHAVFQyrlveftRVDGtagrrvKPYIIDLGSGNGTYLNNQRIEPqryYELKEKDVLKFG 339
Cdd:cd22737  32 DIVIPEGSVSRHHATLV-------PTPG------GTQIRDLRSTNGTFVNGLRVDA---ALLHDGDVVTIG 86

second forkhead associated (FHA) domain found in Saccharomyces cerevisiae Serine/threonine-protein kinase RAD53 and similar proteins; RAD53, also called CHEK2 homolog, or serine-protein kinase 1 (Spk1), is a nuclear protein kinase that phosphorylates proteins on serine, threonine, and tyrosine. It controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints and prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5. It may be involved in the phosphorylation of RPH1. RAD53 contains two FHA domains. This model corresponds to the second one. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633 255 RQSAYLLGRLRKIaDIPIDHPSCSKQHAVFqyrlvefTRVDGTAGRRVKpYIIDLGSgNGTYLNNQRIEPQRYYELKEKD 334
Cdd:cd22690  17 TQNTTFIGRSKDC-DEEITDPRISKHHCII-------TRKRSGKGLDDV-YVTDTST-NGTFINNNRLGKGSQSLLQDGD 86

                ....
gi 70887633 335 VLKF 338
Cdd:cd22690  87 EIVL 90

transcription termination factor Rho; Provisional

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70887633   26 PARPPRARRHSSGSSRDSSSPAQRRRDSRSPVRRKDRSPARRDRSSGRQQERSPRPRRSRSPHRNTEARIKREQDDPRG- 104
Cdd:PRK12678  65 AAAATPAAPAAAARRAARAAAAARQAEQPAAEAAAAKAEAAPAARAAAAAAAEAASAPEAAQARERRERGEAARRGAARk 144

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 70887633  105 -SESHRHRERRDDSNDRRLKREAERPRQRDRGREQDRQRGRIRDAHIQEQQQAERERHNERRRENRLRQEAQSGGAEE 181
Cdd:PRK12678 145 aGEGGEQPATEARADAAERTEEEERDERRRRGDREDRQAEAERGERGRREERGRDGDDRDRRDRREQGDRREERGRRD 222