BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ (poxvirus and zinc finger) domain found in Drosophila melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2 (BAB2), modifier of mdg4 (doom), and similar proteins; BAB1 and BAB2 probably act as transcriptional regulators that are required for specification of the tarsal segment and are involved in antenna development. Doom is a product of the Drosophila mod(mdg4) gene. It induces apoptosis and binds to baculovirus inhibitor-of-apoptosis proteins. This subfamily also includes Drosophila melanogaster sex determination protein fruitless (FRU), protein jim lovell (LOV), zinc finger protein chinmo, transcription factor GAGA, transcription factor Ken, and longitudinals lacking proteins (LOLA). FRU probably acts as a transcriptional regulator that plays a role in male courtship behavior and sexual orientation, and enhances male-specific expression of takeout in brain-associated fat body. LOV, also called tyrosine kinase-related (TKR), has a regulatory role during midline cell development. Chinmo is a functional effector of the JAK/STAT pathway that regulates eye development, tumor formation, and stem cell self-renewal in Drosophila. GAGA is a transcriptional activator that functions by regulating chromatin structure. Ken, also termed protein Ken and Barbie, is a transcription factor required for Terminalia development. LOLA proteins are putative transcription factors required for axon growth and guidance in the central and peripheral nervous systems. Proteins in this subfamily contain a BTB domain. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   32 FVDVILTCVGspenhlsdeavaRHIPANRSVLAAASPVFAAMFCENPLvEEDVRISVVGYSYTSIRSIIEYIYTGSLEFS 111
Cdd:cd18315      1 LVDVTLACEG------------GSLKAHKLVLAAASPYFAALLKETPP-DEHPVIILPDVPYSELKALLDFIYTGEVNVS 67

                           90
                   ....*....|....*.
gi 1022773073  112 DCDKEQIKEILRDFLI 127
Cdd:cd18315     68 QEQLESLLKLAELLQI 83

FOG: Zn-finger [General function prediction only];

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073  691 NHLCEQCGRSFRFARYLNRHMSLHSSEKPHVCRY--CDKAFRLKPVLIKHIRTHTGEKPYKCTvcgRCFRQRTTYATHLK 768
Cdd:COG5048     33 PDSCPNCTDSFSRLEHLTRHIRSHTGEKPSQCSYsgCDKSFSRPLELSRHLRTHHNNPSDLNS---KSLPLSNSKASSSS 109

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073  769 MHEK----RQMAGVREGNTGLGQLPRLKCDFCSMTFKLDLFL----------------------MLHRAKHTGETPALPC 822
Cdd:COG5048    110 LSSSssnsNDNNLLSSHSLPPSSRDPQLPDLLSISNLRNNPLpgnnsssvntpqsnslhpplpaNSLSKDPSSNLSLLIS 189

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073  823 PTCKDTFPSIKELTLHRKSQHPESVYACQICPKVFTN--KANFDFHVHKHSNPDAVKKKYPKVDPVDTGPCtcdfcdkGI 900
Cdd:COG5048    190 SNVSTSIPSSSENSPLSSSYSIPSSSSDQNLENSSSSlpLTTNSQLSPKSLLSQSPSSLSSSDSSSSASES-------PR 262

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073  901 HLGDKSLKCQYcdkTFSHKPSLEQHMRTHtgerPFKCDQCPSSFKQQQHLTAHM--VVHTGE--RPFSCDH--CDKTFPH 974
Cdd:COG5048    263 SSLPTASSQSS---SPNESDSSSEKGFSL----PIKSKQCNISFSRSSPLTRHLrsVNHSGEslKPFSCPYslCGKLFSR 335

                          330
                   ....*....|....*
gi 1022773073  975 KTSLYAHLRIHTGKR 989
Cdd:COG5048    336 NDALKRHILLHTSIS 350

BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ (poxvirus and zinc finger) domain found in Drosophila melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2 (BAB2), modifier of mdg4 (doom), and similar proteins; BAB1 and BAB2 probably act as transcriptional regulators that are required for specification of the tarsal segment and are involved in antenna development. Doom is a product of the Drosophila mod(mdg4) gene. It induces apoptosis and binds to baculovirus inhibitor-of-apoptosis proteins. This subfamily also includes Drosophila melanogaster sex determination protein fruitless (FRU), protein jim lovell (LOV), zinc finger protein chinmo, transcription factor GAGA, transcription factor Ken, and longitudinals lacking proteins (LOLA). FRU probably acts as a transcriptional regulator that plays a role in male courtship behavior and sexual orientation, and enhances male-specific expression of takeout in brain-associated fat body. LOV, also called tyrosine kinase-related (TKR), has a regulatory role during midline cell development. Chinmo is a functional effector of the JAK/STAT pathway that regulates eye development, tumor formation, and stem cell self-renewal in Drosophila. GAGA is a transcriptional activator that functions by regulating chromatin structure. Ken, also termed protein Ken and Barbie, is a transcription factor required for Terminalia development. LOLA proteins are putative transcription factors required for axon growth and guidance in the central and peripheral nervous systems. Proteins in this subfamily contain a BTB domain. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   32 FVDVILTCVGspenhlsdeavaRHIPANRSVLAAASPVFAAMFCENPLvEEDVRISVVGYSYTSIRSIIEYIYTGSLEFS 111
Cdd:cd18315      1 LVDVTLACEG------------GSLKAHKLVLAAASPYFAALLKETPP-DEHPVIILPDVPYSELKALLDFIYTGEVNVS 67

                           90
                   ....*....|....*.
gi 1022773073  112 DCDKEQIKEILRDFLI 127
Cdd:cd18315     68 QEQLESLLKLAELLQI 83

Broad-Complex, Tramtrack and Bric a brac; Domain in Broad-Complex, Tramtrack and Bric a brac. Also known as POZ (poxvirus and zinc finger) domain. Known to be a protein-protein interaction motif found at the N-termini of several C2H2-type transcription factors as well as Shaw-type potassium channels. Known structure reveals a tightly intertwined dimer formed via interactions between N-terminal strand and helix structures. However in a subset of BTB/POZ domains, these two secondary structures appear to be missing. Be aware SMART predicts BTB/POZ domains without the beta1- and alpha1-secondary structures.

                            10        20        30        40        50        60        70
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1022773073    54 RHIPANRSVLAAASPVFAAMFCENPLVEEDVRISVVGYSYTSIRSIIEYIYTGSLEFSDCDKEQIKEILRDFLIP 128
Cdd:smart00225    9 KKFHAHKAVLAAHSPYFKALFSSDFKESDKSEIYLDDVSPEDFRALLNFLYTGKLDLPEENVEELLELADYLQIP 83

FOG: Zn-finger [General function prediction only];

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073  691 NHLCEQCGRSFRFARYLNRHMSLHSSEKPHVCRY--CDKAFRLKPVLIKHIRTHTGEKPYKCTvcgRCFRQRTTYATHLK 768
Cdd:COG5048     33 PDSCPNCTDSFSRLEHLTRHIRSHTGEKPSQCSYsgCDKSFSRPLELSRHLRTHHNNPSDLNS---KSLPLSNSKASSSS 109

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073  769 MHEK----RQMAGVREGNTGLGQLPRLKCDFCSMTFKLDLFL----------------------MLHRAKHTGETPALPC 822
Cdd:COG5048    110 LSSSssnsNDNNLLSSHSLPPSSRDPQLPDLLSISNLRNNPLpgnnsssvntpqsnslhpplpaNSLSKDPSSNLSLLIS 189

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073  823 PTCKDTFPSIKELTLHRKSQHPESVYACQICPKVFTN--KANFDFHVHKHSNPDAVKKKYPKVDPVDTGPCtcdfcdkGI 900
Cdd:COG5048    190 SNVSTSIPSSSENSPLSSSYSIPSSSSDQNLENSSSSlpLTTNSQLSPKSLLSQSPSSLSSSDSSSSASES-------PR 262

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073  901 HLGDKSLKCQYcdkTFSHKPSLEQHMRTHtgerPFKCDQCPSSFKQQQHLTAHM--VVHTGE--RPFSCDH--CDKTFPH 974
Cdd:COG5048    263 SSLPTASSQSS---SPNESDSSSEKGFSL----PIKSKQCNISFSRSSPLTRHLrsVNHSGEslKPFSCPYslCGKLFSR 335

                          330
                   ....*....|....*
gi 1022773073  975 KTSLYAHLRIHTGKR 989
Cdd:COG5048    336 NDALKRHILLHTSIS 350

N-terminal domain of Oryza sativa transcription factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana SUF4 (AtSUF4), and similar proteins; Oryza sativa SUPPRESSOR OF FRI 4 (OsSUF4) is a C2H2-type zinc finger transcription factor which interacts with the major H3K36 methyltransferase SDG725 to promote H3K36me3 (tri-methylation at H3K9) establishment. The transcription factor OsSUF4 recognizes a specific 7-bp DNA element (5'-CGGAAAT-3'), which is contained in the promoter regions of many genes throughout the rice genome. Through interaction with OsSUF4, SDG725 is recruited to the promoters of key florigen genes, RICE FLOWERING LOCUS T1 (RFT1) and Heading date 3a (Hd3a), for H3K36 deposition to promote gene activation and rice plant flowering. OsSUF4 target genes include a number of genes involved in many biological processes. Flowering plant Arabidopsis SUF4 binds to a 15bp DNA element (5'-CCAAATTTTAAGTTT-3') within the promoter of the floral repressor gene FLOWERING LOCUS C (FLC) and recruits the FRI-C transcription activator complex to the FLC promoter. Although the DNA-binding element and target genes of AtSUF4 are different from those of OsSUF4, AtSUF4 is known to interact with the Arabidopsis H3K36 methyltransferase SDG8 (also known as ASHH2/EFS/SET8), and the methylation deposition mechanism mediated by the SUF4 transcription factor and H3K36 methyltransferase may be conserved in Arabidopsis and rice. Proteins in this family have two conserved C2H2-type zinc finger motifs at the N-terminus (included in this model), and a large proline-rich domain at the C-terminus; for OsSUF4, it has been shown that the N-terminal zinc-finger domain is responsible for DNA binding, and that the C-terminal domain interacts with SDG725.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|.
gi 1022773073  908 KCQYCDKTFSHKPSLEQHMRTHTgerpFKCDQCPSSFKQQQHLTAHMV-VH 957
Cdd:cd20908      3 WCYYCDREFDDEKILIQHQKAKH----FKCHICHKKLYTAGGLAVHCLqVH 49

BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ (poxvirus and zinc finger) domain found in Drosophila melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2 (BAB2), modifier of mdg4 (doom), and similar proteins; BAB1 and BAB2 probably act as transcriptional regulators that are required for specification of the tarsal segment and are involved in antenna development. Doom is a product of the Drosophila mod(mdg4) gene. It induces apoptosis and binds to baculovirus inhibitor-of-apoptosis proteins. This subfamily also includes Drosophila melanogaster sex determination protein fruitless (FRU), protein jim lovell (LOV), zinc finger protein chinmo, transcription factor GAGA, transcription factor Ken, and longitudinals lacking proteins (LOLA). FRU probably acts as a transcriptional regulator that plays a role in male courtship behavior and sexual orientation, and enhances male-specific expression of takeout in brain-associated fat body. LOV, also called tyrosine kinase-related (TKR), has a regulatory role during midline cell development. Chinmo is a functional effector of the JAK/STAT pathway that regulates eye development, tumor formation, and stem cell self-renewal in Drosophila. GAGA is a transcriptional activator that functions by regulating chromatin structure. Ken, also termed protein Ken and Barbie, is a transcription factor required for Terminalia development. LOLA proteins are putative transcription factors required for axon growth and guidance in the central and peripheral nervous systems. Proteins in this subfamily contain a BTB domain. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   32 FVDVILTCVGspenhlsdeavaRHIPANRSVLAAASPVFAAMFCENPLvEEDVRISVVGYSYTSIRSIIEYIYTGSLEFS 111
Cdd:cd18315      1 LVDVTLACEG------------GSLKAHKLVLAAASPYFAALLKETPP-DEHPVIILPDVPYSELKALLDFIYTGEVNVS 67

                           90
                   ....*....|....*.
gi 1022773073  112 DCDKEQIKEILRDFLI 127
Cdd:cd18315     68 QEQLESLLKLAELLQI 83

BTB/POZ domain; The BTB (for BR-C, ttk and bab) or POZ (for Pox virus and Zinc finger) domain is present near the N-terminus of a fraction of zinc finger (pfam00096) proteins and in proteins that contain the pfam01344 motif such as Kelch and a family of pox virus proteins. The BTB/POZ domain mediates homomeric dimerization and in some instances heteromeric dimerization. The structure of the dimerized PLZF BTB/POZ domain has been solved and consists of a tightly intertwined homodimer. The central scaffolding of the protein is made up of a cluster of alpha-helices flanked by short beta-sheets at both the top and bottom of the molecule. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes including N-CoR and SMRT. The POZ or BTB domain is also known as BR-C/Ttk or ZiN.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   28 RRESFVDVILTCVGspenhlsdeavaRHIPANRSVLAAASPVFAAMFCENPLVEEDVRISVVGYSYTSIRSIIEYIYTGS 107
Cdd:pfam00651    6 EQGELCDVTLVVGD------------KEFRAHKAVLAACSPYFKALFSGQESESSVSEITLDDVSPEDFEALLEFMYTGK 73

                           90
                   ....*....|....*.
gi 1022773073  108 LEfsdcDKEQIKEILR 123
Cdd:pfam00651   74 LI----SEENVDDLLA 85

first BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Rho-related BTB domain-containing proteins (RhoBTB); RhoBTB proteins constitute a subfamily of atypical members within the Rho family of small guanosine triphosphatases (GTPases), which is characterized by containing a GTPase domain (in most cases, non-functional) followed by a proline-rich region, tandem BTB domains, and a conserved C-terminal region. In vertebrates, the RhoBTB subfamily consists of 3 isoforms: RhoBTB1, RhoBTB2, and RhoBTB3. Orthologs are present in several other eukaryotes, such as Drosophila and Dictyostelium, but have been lost in plants and fungi. This model corresponds to the first BTB domain. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   23 LLEWYRRESFVDVILTCVGSpenhlsdeavaRHIPANRSVLAAASPVFAAMFCENPLVEEDVRIsvvgySYTSIRSIIEY 102
Cdd:cd18299      3 LRNLLHSPSCADVVFILQGG-----------VRIFAHRIVLAAASSVFADLFLMMTVVTLDSDI-----TPEAFRRVLEF 66

                           90       100
                   ....*....|....*....|....*.
gi 1022773073  103 IYTGSLEFSDCDKEQIKEILRDFLIP 128
Cdd:cd18299     67 LYTGVLDENEDDLKELKDAAELLELF 92

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in gigaxonin; Gigaxonin, also called Kelch-like protein 16 (KLHL16), may be a cytoskeletal component that directly or indirectly plays an important role in neurofilament architecture. It may also act as a substrate-specific adaptor of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including tubulin folding cofactor B (TBCB), microtubule-associated protein MAP1B, and glial fibrillary acidic protein (GFAP). Gigaxonin is mutated in giant axonal neuropathy. It contains a BTB domain and kelch repeats, characteristics of a kelch family protein. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   44 ENHLSDEAV---ARHIPANRSVLAAASPVFAAMFCENPLVEEDVRISVV--GYSYTSIRSIIEYIYTGSLEFSDcdkEQI 118
Cdd:cd18245     11 EPHLCDATLvvgGEEIPVQKNVLAAASPYFRAKLNYNPPKDDGSTYKIEleGISVEVMEEILDYIYTGQIKLSE---DNI 87

                   ....
gi 1022773073  119 KEIL 122
Cdd:cd18245     88 QDIV 91

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch repeat and BTB domain-containing proteins KBTBD6 and KBTBD7; KBTBD6 and KBTBD7 are substrate adaptors of a cullin-3 RING ubiquitin ligase complex that mediates ubiquitylation and proteasomal degradation of T-lymphoma and metastasis gene 1 (TIAM1), a RAC1-specific guanine exchange factor (GEF), by cooperating with gamma-aminobutyric acid receptor-associated proteins (GABARAP). KBTBD7 may also act as a new transcriptional activator in mitogen-activated protein kinase (MAPK) signaling. They both contain a BTB domain and kelch repeats, characteristics of a kelch family protein. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   15 HASCFERLLLEWYRRESFVDVILTCVGSPEnhlsDEAVARHIPANRSVLAAASPVFAAMFCE--NPLVEEDVRISVVGYS 92
Cdd:cd18273      9 HARALLAQLKSFYDARLLTDVTIEVNGPGS----GPGSGRLFPCNRNVLAAASPYFKSMFTGglYESKQETVTIHDVDSE 84

                           90       100
                   ....*....|....*....|
gi 1022773073   93 ytSIRSIIEYIYTGSLEFSD 112
Cdd:cd18273     85 --SMALIIDYCYTGRVTLSE 102

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Drosophila melanogaster protein roadkill and similar proteins; Drosophila melanogaster protein roadkill, also called Hh-induced MATH and BTB domain-containing protein (HIB), is a hedgehog-induced BTB protein that modulates hedgehog signaling by degrading Ci/Gli transcription factor. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   27 YRRESFVDVILtCVGspenhlsdeavARHIPANRSVLAAASPVFAAMFcENPLVEEDV-RISVVGYSYTSIRSIIEYIYT 105
Cdd:cd18345     13 FERSAFSDVTL-CVG-----------GREFQAHKAILAARSPVFNAMF-EHEMEERKQnRVEITDVDHEVMREMLRFIYT 79

                   .
gi 1022773073  106 G 106
Cdd:cd18345     80 G 80

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Influenza virus NS1A-binding protein (NS1-BP); NS1-BP is also called NS1-binding protein, aryl hydrocarbon receptor-associated protein 3 (ARA3), or IVNS1ABP. It is a novel protein that interacts with the influenza A virus nonstructural NS1 protein, which is relocalized in the nuclei of infected cells. It plays a role in cell division and in the dynamic organization of the actin skeleton as a stabilizer of actin filaments by association with F-actin through its kelch repeats. It also interacts with alpha-enolase/MBP-1 and is involved in c-Myc gene transcriptional control. NS1-BP contains BTB and BACK domains at the N-terminal region and kelch repeats at the C-terminal region. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   32 FVDVILTCVGspenhlsdeavaRHIPANRSVLAAASPVFAAMFceNPLvEEDVRISVV---GYSYTSIRSIIEYIYTGSL 108
Cdd:cd18306     18 FCDVILQVGG------------HEIPAHRAVLACASPYLFELF--SSD-SDGESILTVkldGLDPDAVEVLVNYAYTSRL 82

                           90
                   ....*....|....*
gi 1022773073  109 EfsdCDKEQIKEILR 123
Cdd:cd18306     83 E---VPADLVKSVYS 94

second BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Rho-related BTB domain-containing proteins (RhoBTB); RhoBTB proteins constitute a subfamily of atypical members within the Rho family of small guanosine triphosphatases (GTPases), which is characterized by containing a GTPase domain (in most cases, non-functional) followed by a proline-rich region, a tandem of 2 BTB domains, and a conserved C-terminal region. In humans, the RhoBTB subfamily consists of 3 isoforms: RhoBTB1, RhoBTB2, and RhoBTB3. Orthologs are present in several other eukaryotes, such as Drosophila and Dictyostelium, but have been lost in plants and fungi. This model corresponds to the second BTB domain. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   56 IPANRSVLAAASPVFAAMFCENPLVEEDVRISVVGYSYTSIRSIIEYIYTGSLEFSDCDK 115
Cdd:cd18300     21 IPAHKALLVARCDVMAAMFGGNFRESSAKEVELPGVSKETFLALLEYLYTDQAPILEDGD 80

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch-like protein 4 (KLHL4); KLHL4 shares high identity and similarity with the Drosophila kelch protein, a component of ring canals. It may be associated with X-linked cleft palate (CPX) and is also a candidate gene in the impairment of mullerian duct development. In addition, it has been identified as a target of insulin-like growth factor binding protein 5 (IGFBP5). KLHL4 contains a BTB domain and kelch repeat domains, characteristics of a kelch family protein. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 1022773073   56 IPANRSVLAAASPVFAAMFCENPLVEEDVRISVVGYSYTSIRSIIEYIYTGSLEFSD 112
Cdd:cd18336     31 IPAHRLVLSAVSDYFAAMFTNDVREAKQEEIKMEGVDPDALKALVHYAYTGVLELKE 87

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch-like protein 24 (KLHL24); KLHL24, also called kainate receptor-interacting protein for GluR6 (KRIP6) or protein DRE1, is necessary to maintain the balance between intermediate filament stability and degradation, a process that is essential for skin integrity. KLHL24 is a component of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that mediates ubiquitination of KRT14 and controls its levels during keratinocyte differentiation. It contains a BTB domain and kelch repeats, characteristics of a kelch family protein. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   32 FVDVILtCVGSpenhlsdeavaRHIPANRSVLAAASPVFAAMFCENPLVEEDVRISVVGYSYTSIRSIIEYIYTGSLEFS 111
Cdd:cd18253     18 FTDVII-CVEG-----------REFPCHRAILSACSSYFRAMFCNDHRESREMLVEINGILAEAMDCFLQYVYTGKVKIT 85

                   ....*....
gi 1022773073  112 DCDKEQIKE 120
Cdd:cd18253     86 TENVQYLFE 94

FOG: Zn-finger [General function prediction only];

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073  691 NHLCEQCGRSFRFARYLNRHMSLHSSEKPHVCRY--CDKAFRLKPVLIKHIRTHTGEKPYKCTvcgRCFRQRTTYATHLK 768
Cdd:COG5048     33 PDSCPNCTDSFSRLEHLTRHIRSHTGEKPSQCSYsgCDKSFSRPLELSRHLRTHHNNPSDLNS---KSLPLSNSKASSSS 109

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073  769 MHEK----RQMAGVREGNTGLGQLPRLKCDFCSMTFKLDLFL----------------------MLHRAKHTGETPALPC 822
Cdd:COG5048    110 LSSSssnsNDNNLLSSHSLPPSSRDPQLPDLLSISNLRNNPLpgnnsssvntpqsnslhpplpaNSLSKDPSSNLSLLIS 189

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073  823 PTCKDTFPSIKELTLHRKSQHPESVYACQICPKVFTN--KANFDFHVHKHSNPDAVKKKYPKVDPVDTGPCtcdfcdkGI 900
Cdd:COG5048    190 SNVSTSIPSSSENSPLSSSYSIPSSSSDQNLENSSSSlpLTTNSQLSPKSLLSQSPSSLSSSDSSSSASES-------PR 262

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073  901 HLGDKSLKCQYcdkTFSHKPSLEQHMRTHtgerPFKCDQCPSSFKQQQHLTAHM--VVHTGE--RPFSCDH--CDKTFPH 974
Cdd:COG5048    263 SSLPTASSQSS---SPNESDSSSEKGFSL----PIKSKQCNISFSRSSPLTRHLrsVNHSGEslKPFSCPYslCGKLFSR 335

                          330
                   ....*....|....*
gi 1022773073  975 KTSLYAHLRIHTGKR 989
Cdd:COG5048    336 NDALKRHILLHTSIS 350

Zinc-finger double domain;

                           10        20
                   ....*....|....*....|....*
gi 1022773073  735 LIKHIRTHTGEKPYKCTVCGRCFRQ 759
Cdd:pfam13465    2 LKRHMRTHTGEKPYKCPECGKSFKS 26

second BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in BTB/POZ domain-containing protein 8 (BTBD8); BTBD8 is a BTB-domain-containing Kelch-like protein that may play a role in developmental processes. It may also act as a protein-protein adaptor in a transcription complex and thus be involved in brain development. BTBD8 contains two BTB domains. This model corresponds to the second domain. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1022773073   54 RHIPANRSVLAAASPVFAAMFCENPLVEEDVRISVVGYSYTSIRSIIEYIYTGSLEFSdcDKEQIKEILRD 124
Cdd:cd18286     27 KTFKAHRCILCARSSYFAAMLSGSWAESNSSEITLTGVSHAAVSFVLLFIYGGVLDLP--DDVNLGELLSL 95

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch repeat and BTB domain-containing protein 11 (KBTBD11); KBTBD11 is also called chronic myelogenous leukemia-associated protein (CMLAP) or Kelch domain-containing protein 7B, or KLHDC7C. It is a BTB-Kelch family protein whose function remains unclear. A novel polymorphism rs11777210 in KBTBD11 is significantly associated with colorectal cancer risk. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1022773073   44 ENHLSDEAVARHIPANRSVLAAASPVFAAMfcenplVEEDVrISVVGYSYTSIRSIIEYIYTGSLEFSdcdKEQIKEIL 122
Cdd:cd18275      4 EPDLVIEVSGQRIRAHKSVLAAKSDYFRAR------LSRDI-LKVKGLSYATLRLLVDYVYSGRMAVS---KDNVVEVV 72

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch-like protein 8 (KLHL8); KLHL8 is a substrate-specific adaptor of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for the ubiquitination and degradation of rapsyn, a postsynaptic protein required for clustering of nicotinic acetylcholine receptors (nAChRs) at the neuromuscular junction. It contains a BTB domain and kelch repeats, characteristics of a kelch family protein. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   19 FERLLLEWYRRESFVDVILtCVGSpenhlsdeavaRHIPANRSVLAAASPVFAAMFCeNPLVEEDVR-ISVVGYSYTSIR 97
Cdd:cd18238      2 SFKVLHQFYENGELCDVTL-KVGE-----------KSIHCHRLVLACVSPYFRAMFT-SEMAESKQDsITIKDIDEEAVE 68

                           90
                   ....*....|....
gi 1022773073   98 SIIEYIYTGSLEFS 111
Cdd:cd18238     69 LLVDFAYTGKLTLT 82

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch repeat and BTB domain-containing protein 3 (KBTBD3); KBTBD3, also called BTB and kelch domain-containing protein 3 (BKLHD3), contains a BTB domain and kelch repeats, characteristics of a kelch family protein. Its function remains unclear. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   46 HLSDEAvarhIPANRSVLAAASPVFAAMFCENPLVEEDVRISVVGYSYTSIRSIIEYIYTGSLEFSDCDKE---QIKEIL 122
Cdd:cd18271     30 IVKDET----IPCHRCVLAACSDFFRAMFEVNMKERDDGSVTISNLSPKAVKAFLDYAYTGKAEITDDNVEmffQMSSFL 105

                           90
                   ....*....|....*....
gi 1022773073  123 R---------DFLIPIPNT 132
Cdd:cd18271    106 QvsllskacsDFLIKSIDL 124

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in BTB/POZ domain-containing protein 9 (BTBD9); BTBD9 is a risk factor for Restless Legs Syndrome (RLS) encoding a Cullin-3 substrate adaptor. The BTBD9 gene may be associated with antipsychotic-induced RLS in schizophrenia. Mutations in BTBD9 lead to reduced dopamine, increased locomotion and sleep fragmentation. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   55 HIPANRSVLAAASPVFAAMFcENPLVEEDVR-ISVVGYSYTSIRSIIEYIYTGSLEFSDCDKEQIKEILR 123
Cdd:cd18287     33 RFPAHRVILAARSEYFRALL-YGGMRESQQSeIELKDTNAEAFKALLKYIYTGRLTLTDLKEDVLLDVLG 101

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch-like protein 21 (KLHL21); KLHL21 is a substrate-specific adaptor of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of aurora B. KLHL21 also targets IkappaB kinase-beta to regulate nuclear factor kappa-light chain enhancer of activated B cells (NF-kappaB) signaling negatively. It contains a BTB domain and kelch repeats, characteristics of a kelch family protein. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   14 GHASCFERLLLEWYRRESFVDVILtCVGspenhlsdeavARHIPANRSVLAAASPVFAAMFCENPLVEEDVRISVVGYSY 93
Cdd:cd18250      3 AHALHLLRGLSELRAERKFFDVTL-CAG-----------GREFPCHRTVLAAASSYFRAMFAGELRESRADRVVLHGVSA 70

                           90
                   ....*....|...
gi 1022773073   94 TSIRSIIEYIYTG 106
Cdd:cd18250     71 EILGLLLDFSYTG 83

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch-like protein 22 (KLHL22); KLHL22 is a substrate-specific adaptor of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of polo-like kinase 1 (PLK1) at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates mono-ubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. It contains a BTB domain and kelch repeats, characteristics of a kelch family protein. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1022773073   54 RHIPANRSVLAAASPVFAAMFCENPLVEEDVRISVVGYSYTSIRSIIEYIYTGSLEFSdcdKEQIKEIL 122
Cdd:cd18251     33 KPIEAHRILLAASCDYFRGMFAGGLREMQQTEVLIHGVSYNAMCKILDFIYTSELELS---LDNVQETL 98

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Ankyrin repeat and BTB/POZ domain-containing protein 2 (ABTB2) and similar proteins; This family includes ABTB2, BTBD11, plant ARM repeat protein interacting with ABF2 (ARIA), and similar proteins. ABTB2, also called bood POZ containing gene type 2 (BPOZ-2), is a scaffold protein that controls the degradation of many biological proteins ranging from embryonic development to tumor progression. It may be involved in the initiation of hepatocyte growth. ABTB2 functions as an adaptor protein for the E3 ubiquitin ligase scaffold protein Cullin-3. It directly binds to eukaryotic elongation factor 1A1 (eEF1A1) to promote eEF1A1 ubiquitylation and degradation, and prevent translation. The BTBD11 gene has been recently identified as an all-trans retinoic acid (atRA)-responsive gene that lies downstream of atRA and its receptors in the regulation of neurite outgrowth and cell adhesion in neural as well as non-neural tissues. ARIA is an armadillo (ARM) repeat and BTB domain-containing protein that acts as a positive regulator of ABA response via the modulation of the transcriptional activity of ABF2. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1022773073   54 RHIPANRSVLAAASPVFAAMFcENPLVEEDVRISVVGY-SYTSIRSIIEYIYTGSLEFSDCDKEQIKEILR 123
Cdd:cd18297     22 RPFYAHKIVLVTASDRFKSML-SSGSTEAQTPVIEIPDiRYDIFQLMMQYLYTGGVESLDVAQDDALELLR 91

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.

                           10        20
                   ....*....|....*....|..
gi 1022773073  908 KCQYCDKTFSHKPSLEQHMRTH 929
Cdd:pfam00096    2 KCPDCGKSFSRKSNLKRHLRTH 23

Zinc-finger double domain;

                           10        20
                   ....*....|....*....|....
gi 1022773073  706 YLNRHMSLHSSEKPHVCRYCDKAF 729
Cdd:pfam13465    1 NLKRHMRTHTGEKPYKCPECGKSF 24

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch-like protein 1 (KLHL1); KLHL1 is a neuronal actin-binding protein that modulates voltage-gated CaV2.1 (P/Q-type) and CaV3.2 (alpha1H T-type) calcium channels. It may play a role in organizing the actin cytoskeleton the brain cells. KLHL1 contains a BTB domain and kelch repeat domains, characteristics of a kelch family protein. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   15 HASCFERLLLEWYRRESFVDVILTcvgspenhlsdeAVARHIPANRSVLAAASPVFAAMFCENPLVEEDVRISVVGYSYT 94
Cdd:cd18335      2 HAEQTFRKMESYLKQQQLCDVILI------------AGNRKIPAHRLVLSAVSDYFAAMFTSDVCEAKQEEIKMEGIDPN 69

                           90       100
                   ....*....|....*....|....
gi 1022773073   95 SIRSIIEYIYTGSLEFSDCDKEQI 118
Cdd:cd18335     70 ALWDLVQFAYTGCLELKEDTIENL 93

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch repeat and BTB domain-containing protein 8 (KBTBD8); KBTBD8, also called T-cell activation kelch repeat protein (TA-KRP), is a BTB-kelch family protein that is located in the Golgi apparatus and translocates to the spindle apparatus during mitosis. It acts as a substrate-specific adaptor of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of neural crest specification. The BCR(KBTBD8) complex monoubiquitylates NOLC1 and its paralog TCOF1, the mutation of which underlies the neurocristopathy Treacher Collins syndrome. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   44 ENHLSDEAV----ARHIPANRSVLAAASPVFAAMFCENPLVEEDVRISVVGYSYTSIRSIIEYIYTGSLEFSDCDKEQIK 119
Cdd:cd18274     19 EGQLTDIVVevdhGKTFSCHRNVLAAISPYFRSMFTSGLTESTQKEVRIVGVEAESMHLVLDYAYTSRVTLTEANVQALF 98

                   ....*....
gi 1022773073  120 EILRDFLIP 128
Cdd:cd18274     99 TAASIFQIP 107

FOG: Zn-finger [General function prediction only];

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073  627 SNDADEKVPCTVCNKLIKKTWLKQHMVILHTEERNHLCDECGRTFVIKAQLRAHQ-----LRQHKAVPGNHLCEQCGRSF 701
Cdd:COG5048    220 LENSSSSLPLTTNSQLSPKSLLSQSPSSLSSSDSSSSASESPRSSLPTASSQSSSpnesdSSSEKGFSLPIKSKQCNISF 299

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073  702 RFARYLNRHM--SLHSSE--KPHVCRY--CDKAFRLKPVLIKHIRTHTGEKPYKCtvcgrCFRQRTTYATHLKMHEKRQM 775
Cdd:COG5048    300 SRSSPLTRHLrsVNHSGEslKPFSCPYslCGKLFSRNDALKRHILLHTSISPAKE-----KLLNSSSKFSPLLNNEPPQS 374

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073  776 AGVREGNTGLGQLPRLKcDFCSMTFKLDLFLMLHRAKH-TGETPALPCPTCKDTFPSIKELTLHRKSqHPESVYACQICP 854
Cdd:COG5048    375 LQQYKDLKNDKKSETLS-NSCIRNFKRDSNLSLHIITHlSFRPYNCKNPPCSKSFNRHYNLIPHKKI-HTNHAPLLCSIL 452

                          250
                   ....*....|..
gi 1022773073  855 KVFTNKANFDFH 866
Cdd:COG5048    453 KSFRRDLDLSNH 464

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in zinc finger and BTB domain-containing protein 47 (ZBTB47); ZBTB47, also called zinc finger protein 651 (ZNF651), is a paralog of ZNF652, a novel zinc-finger transcriptional repressor. It interacts with CBFA2T3 via its carboxy-terminal proline-rich region. CBFA2T3-ZNF651 functions as a transcriptional co-repressor complex. ZBTB47 contains a BTB/POZ domain, a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1022773073   57 PANRSVLAAASPVFAAMFCENPLVEEdVRISVVGYSYTSIRSIIEYIYTGSLEFSDCDKEQI 118
Cdd:cd18231     29 PAHKGVLAAYSQFFHSLFTQNKQLQR-VELSLEALTSQGLQQILNFIYTSKLLVNSCNVQDV 89

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.

                           10        20
                   ....*....|....*....|...
gi 1022773073  963 FSCDHCDKTFPHKTSLYAHLRIH 985
Cdd:pfam00096    1 YKCPDCGKSFSRKSNLKRHLRTH 23

second BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Rho-related BTB domain-containing protein 3 (RhoBTB3); RhoBTB3 is an atypical Rho family small guanosine triphosphatase (GTPase) and is a member of the RhoBTB subfamily, which is characterized by containing a GTPase domain (in most cases, non-functional) followed by a proline-rich region, tandem BTB domains, and a conserved C-terminal region. The carboxyl terminal extension that harbors two BTB domains is capable of assembling cullin 3-dependent ubiquitin ligase complexes. RhoBTB3 is a Golgi-associated Rho-related ATPase that regulates the S/G2 transition of the cell cycle by targeting cyclin E for ubiquitylation. It is involved in vesicle trafficking and in targeting proteins for degradation in the proteasome. It binds directly to Rab9 GTPase and functions with Rab9 in protein transport from endosomes to the trans Golgi network. It also promotes proteasomal degradation of Hypoxia-inducible factor alpha (HIFalpha) through facilitating hydroxylation and suppresses the Warburg effect. This model corresponds to the second BTB domain. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|..
gi 1022773073   56 IPANRSVLAAASPVFAAMFCENPLVEEDVRISVVGYSYTSIRSIIEYIYTGS 107
Cdd:cd18360     22 VPAHRAVLVARCEVMAAMFNGNYAEAKSFLVPIYGVSKDTFLSFLEYLYTDS 73

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in speckle-type POZ protein-like (SPOPL); SPOPL, also called HIB homolog 2 or Roadkill homolog 2, is a component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The complexes may contain homodimeric SPOPL or the heterodimers formed by speckle-type POZ protein (SPOP) and SPOPL, which are less efficient than ubiquitin ligase complexes containing only SPOP. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|
gi 1022773073   58 ANRSVLAAASPVFAAMFcENPLVE-EDVRISVVGYSYTSIRSIIEYIYTG 106
Cdd:cd18343     35 AHKSILAARSPVFNAMF-EHEMEEsKKNRVEINDVDPEVFKEMMRFIYTG 83

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch-like proteins, KLHL40 and KLHL41; This family includes Kelch-like proteins, KLHL40 and KLHL41. KLHL40 is a substrate-specific adaptor of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a key regulator of skeletal muscle development. KLHL41 is a novel kelch related protein that is involved in pseudopod elongation in transformed cells. They both contain a BTB domain and kelch repeats, characteristics of a kelch family protein. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1022773073   54 RHIPANRSVLAAASPVFAAMFCEN-------PLVEEDVRISVVGysytsirSIIEYIYTGSLEFSDCDKEQIKEILRDFL 126
Cdd:cd18269     37 KEFPCHRLVLAACSPYFRAMFLSDleeskkkEVVLEDVDPDVMG-------MILKYLYTSEIDLNDQNVQDIFALASRFQ 109

                   ..
gi 1022773073  127 IP 128
Cdd:cd18269    110 IP 111

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in speckle-type POZ protein (SPOP) and similar proteins; This family includes speckle-type POZ protein (SPOP), speckle-type POZ protein-like (SPOPL), TD and POZ domain-containing proteins (TDPOZ), Drosophila melanogaster protein roadkill and similar proteins. Both, SPOP and SPOPL, serve as adaptors of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that mediate the ubiquitination and proteasomal degradation of target proteins. TDPOZ is a family of bipartite animal and plant proteins that contain a tumor necrosis factor receptor-associated factor (TRAF) domain (TD) and a POZ/BTB domains. TDPOZ proteins may be nuclear scaffold proteins probably involved in transcription regulation in early development and other cellular processes. Drosophila melanogaster protein roadkill, also called Hh-induced MATH and BTB domain-containing protein (HIB), is a hedgehog-induced BTB protein that modulates hedgehog signaling by degrading Ci/Gli transcription factor. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1022773073   29 RESFVDVILtCVGspenhlsdeavARHIPANRSVLAAASPVFAAMFcENPLVEEDV-RISVVGYSYTSIRSIIEYIYTG 106
Cdd:cd18279     15 NSRFTDCCL-CVG-----------GQEFQAHKAILAARSPVFSAMF-EHEMEESKKnRVEINDVDPEVFKEMMRFIYTG 80