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Conserved domains on  [gi|844664171|gb|KMJ32354|]
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phospholipid ABC transporter substrate-binding protein [Salmonella enterica subsp. enterica serovar Typhimurium]

Protein Classification

outer membrane lipid asymmetry maintenance protein MlaD( domain architecture ID 10025210)

outer membrane lipid asymmetry maintenance protein MlaD is part of the MlaFEDB complex, a non-canonical ABC transporter involved in maintaining outer membrane asymmetry

Gene Ontology:  GO:1990531|GO:0015914|GO:0005548
PubMed:  19383799|27529189

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
OM_asym_MlaD TIGR04430
outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are ...
 1-140 2.47e-69

outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are the MlaD (maintenance of Lipid Asymmetry D) protein of an ABC transport system that seems to remove phospholipid from the outer leaflet of the Gram-negative bacterial outer membrane (OM), leaving only lipopolysaccharide in the outer leaflet. The Mla locus has long been associated with toluene tolerance, consistent with the proposed role in retrograde transport of phospholipid and therefore with maintaining the integrity of the OM as a protective barrier.


:

Pssm-ID: 275223 [Multi-domain]  Cd Length: 146  Bit Score: 206.94  E-value: 2.47e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 844664171    1 MLVALLAALFVCLKAANVTSMRTEPTYKVYATFDNIGGLKVRSPVRIGGVVVGRVEDISLDPKTYLPRVTLDIEERYnHI 80
Cdd:TIGR04430  13 VLAGAAALLFLALKVGNLSSFSSGDTYTVTASFDNIGGLKVGAPVKIAGVKVGRVTDISLDPKTYQAVVTLSIDKDY-KL 91

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 844664171   81 PDTSSLSIRTSGLLGEQYLALNVGFEDpelgtSILKDGSTIQDTKSAMVLEDMIGQFLYN 140
Cdd:TIGR04430  92 PDDSSASILTSGLLGEQYIGLSPGGDE-----DYLKDGDEIEDTQSAVVLESLIGKFLFS 146
 
Name Accession Description Interval E-value
OM_asym_MlaD TIGR04430
outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are ...
 1-140 2.47e-69

outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are the MlaD (maintenance of Lipid Asymmetry D) protein of an ABC transport system that seems to remove phospholipid from the outer leaflet of the Gram-negative bacterial outer membrane (OM), leaving only lipopolysaccharide in the outer leaflet. The Mla locus has long been associated with toluene tolerance, consistent with the proposed role in retrograde transport of phospholipid and therefore with maintaining the integrity of the OM as a protective barrier.


Pssm-ID: 275223 [Multi-domain]  Cd Length: 146  Bit Score: 206.94  E-value: 2.47e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 844664171    1 MLVALLAALFVCLKAANVTSMRTEPTYKVYATFDNIGGLKVRSPVRIGGVVVGRVEDISLDPKTYLPRVTLDIEERYnHI 80
Cdd:TIGR04430  13 VLAGAAALLFLALKVGNLSSFSSGDTYTVTASFDNIGGLKVGAPVKIAGVKVGRVTDISLDPKTYQAVVTLSIDKDY-KL 91

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 844664171   81 PDTSSLSIRTSGLLGEQYLALNVGFEDpelgtSILKDGSTIQDTKSAMVLEDMIGQFLYN 140
Cdd:TIGR04430  92 PDDSSASILTSGLLGEQYIGLSPGGDE-----DYLKDGDEIEDTQSAVVLESLIGKFLFS 146
MlaD COG1463
Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall ...
 1-137 2.63e-37

Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441072 [Multi-domain]  Cd Length: 260  Bit Score: 129.06  E-value: 2.63e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 844664171   1 MLVALLAALFVCLKAANVTSmrTEPTYKVYATFDNIGGLKVRSPVRIGGVVVGRVEDISLDPKTYLPRVTLDIEERYnHI 80
Cdd:COG1463   14 VLVGLALLVGLALWLGGLGL--FGDTYTYTAVFDDVGGLKVGSPVRYAGVKVGRVTSIELDPDGYRVRVTLRIDPDV-PI 90

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 844664171  81 PDTSSLSIRTSGLLGEQYLALNVGFEDPELgtsiLKDGSTIQdTKSAMVLEDMIGQF 137
Cdd:COG1463   91 PADSVATIRSSGLLGEKYVELSPGGDSGPP----LADGDVIP-LESPVDLEDLLSQL 142
MlaD pfam02470
MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, ...
 25-104 7.50e-23

MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, an ABC transport system that functions to maintain the asymmetry of the outer membrane. The family also contains the mce (mammalian cell entry) proteins from Mycobacterium tuberculosis. The archetype (Rv0169), was isolated as being necessary for colonization of, and survival within, the macrophage. This family contains proteins of unknown function from other bacteria.


Pssm-ID: 426790 [Multi-domain]  Cd Length: 81  Bit Score: 86.87  E-value: 7.50e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 844664171   25 PTYKVYATFDNIGGLKVRSPVRIGGVVVGRVEDISLDPKTYLPRVTLDIEERYN-HIPDTSSLSIRTSGLLGEQYLALNV 103
Cdd:pfam02470   1 RGYTITAYFDDAGGLEVGAPVRYRGVPVGRVTSIDLDPDGKGVRVTLRIDPDYDvLLPKDSRAGIRSLGLLGGKYIALDP 80


                  .
gi 844664171  104 G 104
Cdd:pfam02470  81 G 81
PLN03094 PLN03094
Substrate binding subunit of ER-derived-lipid transporter; Provisional
 3-96 2.72e-04

Substrate binding subunit of ER-derived-lipid transporter; Provisional


Pssm-ID: 178642 [Multi-domain]  Cd Length: 370  Bit Score: 40.53  E-value: 2.72e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 844664171   3 VALLAALFVCLKAANvtsMRTEPT-YKVYATFDNIGGLKVRSPVRIGGVVVGRVedISLDPKTYLPRVTLDIEERYNHIP 81
Cdd:PLN03094  96 AALLALTLAWLRGFQ---LRSKFRkYQAVFEFPQACGICVGTPVRIRGVTVGNV--VRVRPSLEKIDVVVEVEDDKIVIP 170

                         90
                 ....*....|....*
gi 844664171  82 DTSSLSIRTSGLLGE 96
Cdd:PLN03094 171 RNSLVEVNQSGLIME 185
 
Name Accession Description Interval E-value
OM_asym_MlaD TIGR04430
outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are ...
 1-140 2.47e-69

outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are the MlaD (maintenance of Lipid Asymmetry D) protein of an ABC transport system that seems to remove phospholipid from the outer leaflet of the Gram-negative bacterial outer membrane (OM), leaving only lipopolysaccharide in the outer leaflet. The Mla locus has long been associated with toluene tolerance, consistent with the proposed role in retrograde transport of phospholipid and therefore with maintaining the integrity of the OM as a protective barrier.


Pssm-ID: 275223 [Multi-domain]  Cd Length: 146  Bit Score: 206.94  E-value: 2.47e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 844664171    1 MLVALLAALFVCLKAANVTSMRTEPTYKVYATFDNIGGLKVRSPVRIGGVVVGRVEDISLDPKTYLPRVTLDIEERYnHI 80
Cdd:TIGR04430  13 VLAGAAALLFLALKVGNLSSFSSGDTYTVTASFDNIGGLKVGAPVKIAGVKVGRVTDISLDPKTYQAVVTLSIDKDY-KL 91

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 844664171   81 PDTSSLSIRTSGLLGEQYLALNVGFEDpelgtSILKDGSTIQDTKSAMVLEDMIGQFLYN 140
Cdd:TIGR04430  92 PDDSSASILTSGLLGEQYIGLSPGGDE-----DYLKDGDEIEDTQSAVVLESLIGKFLFS 146
MlaD COG1463
Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall ...
 1-137 2.63e-37

Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441072 [Multi-domain]  Cd Length: 260  Bit Score: 129.06  E-value: 2.63e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 844664171   1 MLVALLAALFVCLKAANVTSmrTEPTYKVYATFDNIGGLKVRSPVRIGGVVVGRVEDISLDPKTYLPRVTLDIEERYnHI 80
Cdd:COG1463   14 VLVGLALLVGLALWLGGLGL--FGDTYTYTAVFDDVGGLKVGSPVRYAGVKVGRVTSIELDPDGYRVRVTLRIDPDV-PI 90

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 844664171  81 PDTSSLSIRTSGLLGEQYLALNVGFEDPELgtsiLKDGSTIQdTKSAMVLEDMIGQF 137
Cdd:COG1463   91 PADSVATIRSSGLLGEKYVELSPGGDSGPP----LADGDVIP-LESPVDLEDLLSQL 142
MlaD pfam02470
MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, ...
 25-104 7.50e-23

MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, an ABC transport system that functions to maintain the asymmetry of the outer membrane. The family also contains the mce (mammalian cell entry) proteins from Mycobacterium tuberculosis. The archetype (Rv0169), was isolated as being necessary for colonization of, and survival within, the macrophage. This family contains proteins of unknown function from other bacteria.


Pssm-ID: 426790 [Multi-domain]  Cd Length: 81  Bit Score: 86.87  E-value: 7.50e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 844664171   25 PTYKVYATFDNIGGLKVRSPVRIGGVVVGRVEDISLDPKTYLPRVTLDIEERYN-HIPDTSSLSIRTSGLLGEQYLALNV 103
Cdd:pfam02470   1 RGYTITAYFDDAGGLEVGAPVRYRGVPVGRVTSIDLDPDGKGVRVTLRIDPDYDvLLPKDSRAGIRSLGLLGGKYIALDP 80


                  .
gi 844664171  104 G 104
Cdd:pfam02470  81 G 81
Mtu_fam_mce TIGR00996
virulence factor Mce family protein; Members of this paralogous family are found as six tandem ...
 2-122 1.62e-14

virulence factor Mce family protein; Members of this paralogous family are found as six tandem homologous proteins in the same orientation per cassette, in four separate cassettes in Mycobacterium tuberculosis. The six members of each cassette represent six subfamilies. One subfamily includes the protein mce (mycobacterial cell entry), a virulence protein required for invasion of non-phagocytic cells. [Cellular processes, Pathogenesis]


Pssm-ID: 273384 [Multi-domain]  Cd Length: 291  Bit Score: 69.62  E-value: 1.62e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 844664171    2 LVALLAALFVCLKAANVTSMR----TEPTYKVYATFDNIGGLKVRSPVRIGGVVVGRVEDISLDPKTYlpRVTLDIEERY 77
Cdd:TIGR00996   4 TVAVVVLALVVVLAVVFLRLPftgfGPGTTTVVAYFTDAGGLYPGDKVRVAGVPVGKVTAIEPRGARA--RVTFSLDRSV 81

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 844664171   78 NhIPDTSSLSIRTSGLLGEQYLALNVGfEDPELGTsiLKDGSTIQ 122
Cdd:TIGR00996  82 T-LPANATAAIRTTTLLGSRYIELTPP-GGPPGGR--LSDGGTIP 122
PLN03094 PLN03094
Substrate binding subunit of ER-derived-lipid transporter; Provisional
 3-96 2.72e-04

Substrate binding subunit of ER-derived-lipid transporter; Provisional


Pssm-ID: 178642 [Multi-domain]  Cd Length: 370  Bit Score: 40.53  E-value: 2.72e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 844664171   3 VALLAALFVCLKAANvtsMRTEPT-YKVYATFDNIGGLKVRSPVRIGGVVVGRVedISLDPKTYLPRVTLDIEERYNHIP 81
Cdd:PLN03094  96 AALLALTLAWLRGFQ---LRSKFRkYQAVFEFPQACGICVGTPVRIRGVTVGNV--VRVRPSLEKIDVVVEVEDDKIVIP 170

                         90
                 ....*....|....*
gi 844664171  82 DTSSLSIRTSGLLGE 96
Cdd:PLN03094 171 RNSLVEVNQSGLIME 185
PqiB COG3008
Intermembrane transporter PqiABC subunit PqiB [Cell wall/membrane/envelope biogenesis];
34-102 5.45e-04

Intermembrane transporter PqiABC subunit PqiB [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442245 [Multi-domain]  Cd Length: 542  Bit Score: 39.82  E-value: 5.45e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 844664171  34 DNIGGLKVRSPVRIGGVVVGRVEDISL--DPKTYLPR--VTLDIE-----ERYNHIPDTS-------------SL--SIR 89
Cdd:COG3008  297 DSVRGLSVGAPVEFRGIRVGTVTSVPLeyDPDTQRFRipVLIRIEperlgLRGSRFWASEedwrqlleplvarGLraQLK 376

                         90
                 ....*....|....
gi 844664171  90 TSGLL-GEQYLALN 102
Cdd:COG3008  377 TGNLLtGQLYVDLD 390
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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