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Conserved domains on  [gi|818897823|gb|KKW32124|]
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Restriction endonuclease [Candidatus Uhrbacteria bacterium GW2011_GWA2_52_8d]

Protein Classification

restriction endonuclease( domain architecture ID 11447960)

restriction endonuclease such as Escherichia coli Mrr restriction system protein (EcoKMrr), a type IV restriction endonuclease that recognizes and cleaves N6-methyladenine- and 5-methylcytosine-containing DNA

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Mrr COG1715
Restriction endonuclease Mrr [Defense mechanisms];
185-318 3.35e-27

Restriction endonuclease Mrr [Defense mechanisms];


:

Pssm-ID: 441321  Cd Length: 157  Bit Score: 104.64  E-value: 3.35e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 818897823 185 KILETTDEIEKLKDVEMLKAkLMTVDPYKFEVVVSNLISHVGFSDVKVTKKSGDEGVDVnaLLKNPVSVDLRYSFQVKRW 264
Cdd:COG1715    3 KALAAYEEIRAELAEELLER-LRSLSPYEFEELVAELLRRMGYGSVEVTGRSGDGGIDG--IAKEDRLGGDRIYVQAKRY 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 818897823 265 K-HSVGRNEVANLRGSMDLNN--QGVIIATSHFTESAIHEARSENKtPINLVGIKEL 318
Cdd:COG1715   80 KgNTVGRPEVREFAGALAGEGadKGIFITTSRFTKDAREEARRLGR-RIELIDGDRL 135
 
Name Accession Description Interval E-value
Mrr COG1715
Restriction endonuclease Mrr [Defense mechanisms];
185-318 3.35e-27

Restriction endonuclease Mrr [Defense mechanisms];


Pssm-ID: 441321  Cd Length: 157  Bit Score: 104.64  E-value: 3.35e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 818897823 185 KILETTDEIEKLKDVEMLKAkLMTVDPYKFEVVVSNLISHVGFSDVKVTKKSGDEGVDVnaLLKNPVSVDLRYSFQVKRW 264
Cdd:COG1715    3 KALAAYEEIRAELAEELLER-LRSLSPYEFEELVAELLRRMGYGSVEVTGRSGDGGIDG--IAKEDRLGGDRIYVQAKRY 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 818897823 265 K-HSVGRNEVANLRGSMDLNN--QGVIIATSHFTESAIHEARSENKtPINLVGIKEL 318
Cdd:COG1715   80 KgNTVGRPEVREFAGALAGEGadKGIFITTSRFTKDAREEARRLGR-RIELIDGDRL 135
Mrr_cat pfam04471
Restriction endonuclease; Prokaryotic family found in type II restriction enzymes containing ...
 206-318 6.36e-22

Restriction endonuclease; Prokaryotic family found in type II restriction enzymes containing the hallmark (D/E)-(D/E)XK active site. Presence of catalytic residues implicates this region in the enzymatic cleavage of DNA.


Pssm-ID: 461322  Cd Length: 114  Bit Score: 89.17  E-value: 6.36e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 818897823  206 LMTVDPYKFEVVVSNLISHVGFSdVKVTKKSGDEGVDVnALLKNPVSVDlRYSFQVKRWKHSVGRNEVANLRGSMDLNN- 284
Cdd:pfam04471   1 LDKMSPKEFEELVAELLRAMGYK-VEVTGRSGDGGVDL-IARKDPLGLE-RILVQAKRYKNTVGVPAVQELVGALAGYGa 77

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 818897823  285 -QGVIIATSHFTESAIHEARSENktPINLVGIKEL 318
Cdd:pfam04471  78 dKGIFVTTSDFTPDAREFAKALN--GIVLIDGDEL 110
 
Name Accession Description Interval E-value
Mrr COG1715
Restriction endonuclease Mrr [Defense mechanisms];
185-318 3.35e-27

Restriction endonuclease Mrr [Defense mechanisms];


Pssm-ID: 441321  Cd Length: 157  Bit Score: 104.64  E-value: 3.35e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 818897823 185 KILETTDEIEKLKDVEMLKAkLMTVDPYKFEVVVSNLISHVGFSDVKVTKKSGDEGVDVnaLLKNPVSVDLRYSFQVKRW 264
Cdd:COG1715    3 KALAAYEEIRAELAEELLER-LRSLSPYEFEELVAELLRRMGYGSVEVTGRSGDGGIDG--IAKEDRLGGDRIYVQAKRY 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 818897823 265 K-HSVGRNEVANLRGSMDLNN--QGVIIATSHFTESAIHEARSENKtPINLVGIKEL 318
Cdd:COG1715   80 KgNTVGRPEVREFAGALAGEGadKGIFITTSRFTKDAREEARRLGR-RIELIDGDRL 135
Mrr_cat pfam04471
Restriction endonuclease; Prokaryotic family found in type II restriction enzymes containing ...
 206-318 6.36e-22

Restriction endonuclease; Prokaryotic family found in type II restriction enzymes containing the hallmark (D/E)-(D/E)XK active site. Presence of catalytic residues implicates this region in the enzymatic cleavage of DNA.


Pssm-ID: 461322  Cd Length: 114  Bit Score: 89.17  E-value: 6.36e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 818897823  206 LMTVDPYKFEVVVSNLISHVGFSdVKVTKKSGDEGVDVnALLKNPVSVDlRYSFQVKRWKHSVGRNEVANLRGSMDLNN- 284
Cdd:pfam04471   1 LDKMSPKEFEELVAELLRAMGYK-VEVTGRSGDGGVDL-IARKDPLGLE-RILVQAKRYKNTVGVPAVQELVGALAGYGa 77

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 818897823  285 -QGVIIATSHFTESAIHEARSENktPINLVGIKEL 318
Cdd:pfam04471  78 dKGIFVTTSDFTPDAREFAKALN--GIVLIDGDEL 110
COG1787 COG1787
Endonuclease, HJR/Mrr/RecB family [Defense mechanisms];
210-318 1.19e-16

Endonuclease, HJR/Mrr/RecB family [Defense mechanisms];


Pssm-ID: 441393  Cd Length: 119  Bit Score: 74.90  E-value: 1.19e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 818897823 210 DPYKFEVVVSNLISHVGFsDVKVTKKSGDEGVDVnaLLKNPvsvDLRYSFQVKRWKHSVGRNEVANLRGSMDL--NNQGV 287
Cdd:COG1787   18 DGREFEKYLAKLLRKLGY-DVEVTPGSGDFGADL--IATKD---GERIAVQCKRYKSPVGNKAVQEVVAAKAYygADKAV 91

                         90       100       110
                 ....*....|....*....|....*....|.
gi 818897823 288 IIATSHFTESAIHEARSENktpINLVGIKEL 318
Cdd:COG1787   92 VVTNSYFTKSAKELAKSNG---VELIDRDDL 119
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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