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Conserved domains on  [gi|616688137|gb|KAE85764|]
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protein MraZ [Staphylococcus aureus VET0402R]

Protein Classification

division/cell wall cluster transcriptional repressor MraZ( domain architecture ID 11449500)

division/cell wall cluster transcriptional repressor MraZ has been implicated in the regulation of the dcw gene cluster, which contains cell division genes such as ftsZ

CATH:  2.40.330.20
Gene Ontology:  GO:0003700|GO:0006355|GO:0000976|GO:0043565
PubMed:  15146477
SCOP:  4000936

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MraZ COG2001
MraZ, DNA-binding transcriptional regulator and inhibitor of RsmH methyltransferase activity ...
 1-140 1.50e-71

MraZ, DNA-binding transcriptional regulator and inhibitor of RsmH methyltransferase activity [Translation, ribosomal structure and biogenesis];


:

Pssm-ID: 441604 [Multi-domain]  Cd Length: 144  Bit Score: 210.84  E-value: 1.50e-71
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 616688137   1 MFMGEYDHQLDTKGRMIIPSKFRY----DLNERFIITRGLDKCLFGYTLDEWQQIEEKMKTLPMTKKDARKFMRMFFSGA 76
Cdd:COG2001    1 MFLGTYEHKLDAKGRLSIPAKFRDvlaeELGGGLVLTRGLDGCLELYPLEEWEKLEEKLEALPLFSPEARDFQRLFLGGA 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 616688137  77 VEVELDKQGRINIPQNLRKYANLTKECTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLID 140
Cdd:COG2001   81 TEVELDKQGRILIPPNLREYAGLEKEVVLVGAGNRFEIWDPEAWEAYLAEAEEDFAELAEELGD 144
 
Name Accession Description Interval E-value
MraZ COG2001
MraZ, DNA-binding transcriptional regulator and inhibitor of RsmH methyltransferase activity ...
 1-140 1.50e-71

MraZ, DNA-binding transcriptional regulator and inhibitor of RsmH methyltransferase activity [Translation, ribosomal structure and biogenesis];


Pssm-ID: 441604 [Multi-domain]  Cd Length: 144  Bit Score: 210.84  E-value: 1.50e-71
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 616688137   1 MFMGEYDHQLDTKGRMIIPSKFRY----DLNERFIITRGLDKCLFGYTLDEWQQIEEKMKTLPMTKKDARKFMRMFFSGA 76
Cdd:COG2001    1 MFLGTYEHKLDAKGRLSIPAKFRDvlaeELGGGLVLTRGLDGCLELYPLEEWEKLEEKLEALPLFSPEARDFQRLFLGGA 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 616688137  77 VEVELDKQGRINIPQNLRKYANLTKECTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLID 140
Cdd:COG2001   81 TEVELDKQGRILIPPNLREYAGLEKEVVLVGAGNRFEIWDPEAWEAYLAEAEEDFAELAEELGD 144
PRK00326 PRK00326
transcriptional regulator MraZ;
1-122 4.25e-69

transcriptional regulator MraZ;


Pssm-ID: 234728 [Multi-domain]  Cd Length: 139  Bit Score: 204.66  E-value: 4.25e-69
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 616688137   1 MFMGEYDHQLDTKGRMIIPSKFRYDLNE----RFIITRGLDKCLFGYTLDEWQQIEEKMKTLPMTKKDARKFMRMFFSGA 76
Cdd:PRK00326   1 MFRGEYAHNLDAKGRLSIPAKFRDELGEeadgRLVITKGLDGCLLLYPLPEWEKIEEKLAALPLTNPEARAFQRLLLGGA 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*.
gi 616688137  77 VEVELDKQGRINIPQNLRKYANLTKECTVIGVSNRIEIWDRETWND 122
Cdd:PRK00326  81 VEVELDKQGRILIPPNLREYAGLEKEVVLVGQGNKFEIWDPEAWEA 126
TIGR00242 TIGR00242
division/cell wall cluster transcriptional repressor MraZ; Members of this family contain two ...
 1-142 7.91e-58

division/cell wall cluster transcriptional repressor MraZ; Members of this family contain two tandem copies of a domain described by pfam02381. This protein often is found with other genes of the dcw (division cell wall) gene cluster, including mraW, ftsI, murE, murF, ftsW, murG, etc. Recent work shows MraW in E. coli binds an upstream region with three tandem GTGGG repeats separated by 5bp spacers. We find similar sites in other species. [Cellular processes, Cell division, Regulatory functions, DNA interactions]


Pssm-ID: 129345 [Multi-domain]  Cd Length: 142  Bit Score: 176.13  E-value: 7.91e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 616688137    1 MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYTLDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVE 80
Cdd:TIGR00242   1 MFRGATNVTLDAKGRISVPAKYRAFLLESVVTNRGFENCLLLYPLQEWEKIEQKLNALPSTQKDTRRLQRLIFGHATECE 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 616688137   81 LDKQGRINIPQNLRKYANLTKECTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFD 142
Cdd:TIGR00242  81 MDTAGRVLIANNLRNHAKLEKEIVLIGQFNKFEIWDKVLWEQYLAEDEDLETEVAERMKDFS 142
MraZ_C cd16321
C-terminal subdomain of transcriptional regulator MraZ; This family contains the C-terminal ...
 66-121 4.10e-30

C-terminal subdomain of transcriptional regulator MraZ; This family contains the C-terminal domain of proteins of unknown function (UPF0040), implicated in a cellular function of bacterial cell division. It includes protein MraZ which is present in almost all bacteria and appears to be essential for survival. It is found in gene clusters associated with the cellular function of cell division and cell wall biosynthesis, including mraW, ftsI, murE, murF, ftsW and murG. Members of this family contain two tandem copies of the domain; the crystal structure of a member of this family (MPN314) reveals that the two subdomains are related by a pseudo two-fold axis, with each subdomain containing a highly conserved DXXXR sequence motif in close proximity to each other, suggested to form the functional site.


Pssm-ID: 293784  Cd Length: 62  Bit Score: 103.33  E-value: 4.10e-30
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 616688137  66 RKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKECTVIGVSNRIEIWDRETWN 121
Cdd:cd16321    1 RALLRLFLGGAEEVELDKQGRILLPQRLREYAGLEKEVVFVGVGDRFEIWDPERWE 56
MraZ pfam02381
MraZ protein, putative antitoxin-like; This small 70 amino acid domain is found duplicated in ...
 72-122 3.37e-22

MraZ protein, putative antitoxin-like; This small 70 amino acid domain is found duplicated in a family of bacterial proteins. These proteins may be DNA-binding transcription factors (Pers. comm. A Andreeva _ A Murzin). It is likely, due to the similarity of fold, that this family acts as a bacterial antitoxin like the MazE antitoxin family.


Pssm-ID: 426747  Cd Length: 72  Bit Score: 83.52  E-value: 3.37e-22
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 616688137   72 FFSGAVEVELDKQGRINIPQNLRKYANLTKECTVIGVSNRIEIWDRETWND 122
Cdd:pfam02381   1 MFGGATEVELDAKGRIILPAKLREHAGLEKECVVIGQSNCLEIWPRAEWEQ 51
 
Name Accession Description Interval E-value
MraZ COG2001
MraZ, DNA-binding transcriptional regulator and inhibitor of RsmH methyltransferase activity ...
 1-140 1.50e-71

MraZ, DNA-binding transcriptional regulator and inhibitor of RsmH methyltransferase activity [Translation, ribosomal structure and biogenesis];


Pssm-ID: 441604 [Multi-domain]  Cd Length: 144  Bit Score: 210.84  E-value: 1.50e-71
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 616688137   1 MFMGEYDHQLDTKGRMIIPSKFRY----DLNERFIITRGLDKCLFGYTLDEWQQIEEKMKTLPMTKKDARKFMRMFFSGA 76
Cdd:COG2001    1 MFLGTYEHKLDAKGRLSIPAKFRDvlaeELGGGLVLTRGLDGCLELYPLEEWEKLEEKLEALPLFSPEARDFQRLFLGGA 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 616688137  77 VEVELDKQGRINIPQNLRKYANLTKECTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLID 140
Cdd:COG2001   81 TEVELDKQGRILIPPNLREYAGLEKEVVLVGAGNRFEIWDPEAWEAYLAEAEEDFAELAEELGD 144
PRK00326 PRK00326
transcriptional regulator MraZ;
1-122 4.25e-69

transcriptional regulator MraZ;


Pssm-ID: 234728 [Multi-domain]  Cd Length: 139  Bit Score: 204.66  E-value: 4.25e-69
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 616688137   1 MFMGEYDHQLDTKGRMIIPSKFRYDLNE----RFIITRGLDKCLFGYTLDEWQQIEEKMKTLPMTKKDARKFMRMFFSGA 76
Cdd:PRK00326   1 MFRGEYAHNLDAKGRLSIPAKFRDELGEeadgRLVITKGLDGCLLLYPLPEWEKIEEKLAALPLTNPEARAFQRLLLGGA 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*.
gi 616688137  77 VEVELDKQGRINIPQNLRKYANLTKECTVIGVSNRIEIWDRETWND 122
Cdd:PRK00326  81 VEVELDKQGRILIPPNLREYAGLEKEVVLVGQGNKFEIWDPEAWEA 126
TIGR00242 TIGR00242
division/cell wall cluster transcriptional repressor MraZ; Members of this family contain two ...
 1-142 7.91e-58

division/cell wall cluster transcriptional repressor MraZ; Members of this family contain two tandem copies of a domain described by pfam02381. This protein often is found with other genes of the dcw (division cell wall) gene cluster, including mraW, ftsI, murE, murF, ftsW, murG, etc. Recent work shows MraW in E. coli binds an upstream region with three tandem GTGGG repeats separated by 5bp spacers. We find similar sites in other species. [Cellular processes, Cell division, Regulatory functions, DNA interactions]


Pssm-ID: 129345 [Multi-domain]  Cd Length: 142  Bit Score: 176.13  E-value: 7.91e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 616688137    1 MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYTLDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVE 80
Cdd:TIGR00242   1 MFRGATNVTLDAKGRISVPAKYRAFLLESVVTNRGFENCLLLYPLQEWEKIEQKLNALPSTQKDTRRLQRLIFGHATECE 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 616688137   81 LDKQGRINIPQNLRKYANLTKECTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFD 142
Cdd:TIGR00242  81 MDTAGRVLIANNLRNHAKLEKEIVLIGQFNKFEIWDKVLWEQYLAEDEDLETEVAERMKDFS 142
MraZ_C cd16321
C-terminal subdomain of transcriptional regulator MraZ; This family contains the C-terminal ...
 66-121 4.10e-30

C-terminal subdomain of transcriptional regulator MraZ; This family contains the C-terminal domain of proteins of unknown function (UPF0040), implicated in a cellular function of bacterial cell division. It includes protein MraZ which is present in almost all bacteria and appears to be essential for survival. It is found in gene clusters associated with the cellular function of cell division and cell wall biosynthesis, including mraW, ftsI, murE, murF, ftsW and murG. Members of this family contain two tandem copies of the domain; the crystal structure of a member of this family (MPN314) reveals that the two subdomains are related by a pseudo two-fold axis, with each subdomain containing a highly conserved DXXXR sequence motif in close proximity to each other, suggested to form the functional site.


Pssm-ID: 293784  Cd Length: 62  Bit Score: 103.33  E-value: 4.10e-30
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 616688137  66 RKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKECTVIGVSNRIEIWDRETWN 121
Cdd:cd16321    1 RALLRLFLGGAEEVELDKQGRILLPQRLREYAGLEKEVVFVGVGDRFEIWDPERWE 56
MraZ_N cd16320
N-terminal subdomain of transcriptional regulator MraZ; This family contains the N-terminal ...
 3-59 1.48e-24

N-terminal subdomain of transcriptional regulator MraZ; This family contains the N-terminal domain of proteins of unknown function (UPF0040), implicated in a cellular function of bacterial cell division. It includes protein MraZ which is present in almost all bacteria and appears to be essential for survival. It is found in gene clusters associated with the cellular function of cell division and cell wall biosynthesis, including mraW, ftsI, murE, murF, ftsW and murG. Members of this family contain two tandem copies of the domain; the crystal structure of a member of this family (MPN314) reveals that the two subdomains are related by a pseudo two-fold axis, with each subdomain containing a highly conserved DXXXR sequence motif in close proximity to each other, suggested to form the functional site.


Pssm-ID: 293783  Cd Length: 60  Bit Score: 89.10  E-value: 1.48e-24
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 616688137   3 MGEYDHQLDTKGRMIIPSKFRYDL---NERFIITRGLDKCLFGYTLDEWQQIEEKMKTLP 59
Cdd:cd16320    1 LGTYEHKLDAKGRLSIPAKFREELgagGEGLVLTRGLDGCLELYPLEEWEKLEEKLEALP 60
MraZ pfam02381
MraZ protein, putative antitoxin-like; This small 70 amino acid domain is found duplicated in ...
 72-122 3.37e-22

MraZ protein, putative antitoxin-like; This small 70 amino acid domain is found duplicated in a family of bacterial proteins. These proteins may be DNA-binding transcription factors (Pers. comm. A Andreeva _ A Murzin). It is likely, due to the similarity of fold, that this family acts as a bacterial antitoxin like the MazE antitoxin family.


Pssm-ID: 426747  Cd Length: 72  Bit Score: 83.52  E-value: 3.37e-22
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 616688137   72 FFSGAVEVELDKQGRINIPQNLRKYANLTKECTVIGVSNRIEIWDRETWND 122
Cdd:pfam02381   1 MFGGATEVELDAKGRIILPAKLREHAGLEKECVVIGQSNCLEIWPRAEWEQ 51
MraZ pfam02381
MraZ protein, putative antitoxin-like; This small 70 amino acid domain is found duplicated in ...
 1-70 1.24e-18

MraZ protein, putative antitoxin-like; This small 70 amino acid domain is found duplicated in a family of bacterial proteins. These proteins may be DNA-binding transcription factors (Pers. comm. A Andreeva _ A Murzin). It is likely, due to the similarity of fold, that this family acts as a bacterial antitoxin like the MazE antitoxin family.


Pssm-ID: 426747  Cd Length: 72  Bit Score: 74.28  E-value: 1.24e-18
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 616688137    1 MFMGEYDHQLDTKGRMIIPSKFRYDLN-ERFIITRGLDKCLFGYTLDEWQQIEEKMKTLPMTKKDARKFMR 70
Cdd:pfam02381   1 MFGGATEVELDAKGRIILPAKLREHAGlEKECVVIGQSNCLEIWPRAEWEQYEAELSEESFSAKAERALLR 71
MraZ cd16319
protein domain of unknown function (UPF0040) includes MraZ; This family contains proteins of ...
 79-122 4.53e-04

protein domain of unknown function (UPF0040) includes MraZ; This family contains proteins of unknown function (UPF0040), implicated in a cellular function of bacterial cell division. It includes protein MraZ which is present in almost all bacteria and appears to be essential for survival. It is found in gene clusters associated with the cellular function of cell division and cell wall biosynthesis. Members of this family contain two tandem copies of the domain; the crystal structure of a member of this family (MPN314) reveals that the two subdomains are related by a pseudo two-fold axis, with each subdomain containing a highly conserved DXXXR sequence motif in close proximity to each other, suggested to form the functional site.


Pssm-ID: 293782  Cd Length: 53  Bit Score: 36.12  E-value: 4.53e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*
gi 616688137  79 VELDKQGRINIPQNLRKYANLTKECTVI-GVSNRIEIWDRETWND 122
Cdd:cd16319    4 IDLDAAGRILIPAKLIADAELDGEIVIIgGFEDCLEIRDKKDFED 48
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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